PMC:1084331 / 23550-24949 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1084331","sourcedb":"PMC","sourceid":"1084331","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1084331","text":"Loss of Trk Receptor Expression and Increased Survival in DRG Neurons upon Precocious ETS Signaling\n(A–C and G–I) In situ hybridization analysis of TrkA (A and G), TrkB (B and H), and TrkC (C and I) expression in E16.5 lumbar DRG of wild-type (A–C) and TauEWS-Pea3/+ Isl1Cre/+ (G–I) embryos.\n(D–F and J–L) Analysis of lumbar DRG of wild-type (D), TaumGFP/+ IslCre/+ (E and F), and TauEWS-Pea3/+ Isl1Cre/+ (J, K, and L) embryos for (1) neuronal cell death at E13.5 by TUNEL (green; D and J), (2) cell survival and proliferation at E16.5 by LacZ (blue) wholemount staining (E and K; lumbar levels L1 and L2 are shown), and (3) BrdU (green)/LacZ (red) double labeling (F and L).\n(M and N) Quantitative analysis (n ≥ 3 independent experiments) of the mean number of apoptotic events relative to wild-type levels is shown in (M) and neuronal survival in (N) as percent of wild-type of DRG at lumbar levels L1 to L5 as quantified on serial sections.\n(O) Western blot analysis of protein extracts isolated from lumbar DRG of E16.5 wild-type (wt) and TauEWS-Pea3/+ Isl1Cre/+ (mut) embryos using the following antibodies: Akt, p-Akt (Ser473), CREB, p-CREB (Ser133), Bax, Bcl2, and Bcl-xl.\n(P) Quantitative analysis of protein levels relative to wild-type in percent is shown on the right (n = 3 independent experiments).\nScale bar: (A–C and G–I), 35 μm; (D and J), 40 μm; (E and K), 200 μm; (F and L), 50 μm.","divisions":[{"label":"title","span":{"begin":0,"end":99}},{"label":"p","span":{"begin":100,"end":291}},{"label":"p","span":{"begin":292,"end":675}},{"label":"p","span":{"begin":676,"end":943}},{"label":"p","span":{"begin":944,"end":1179}},{"label":"p","span":{"begin":1180,"end":1311}}],"tracks":[]}