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begin to assess at which step of the neurotrophin signaling cascade DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos become unresponsive to the addition of neurotrophins, we assayed the expression of neurotrophin receptors in TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure 6). Whereas expression of the neurotrophin receptors TrkA, TrkB, and TrkC marks afferents of distinct sensory modalities in DRG of wild-type embryos (Figure 6A–6C) [4,29], TauEWS-Pea3/+ Isl1Cre/+ embryos showed complete absence of expression of TrkA, TrkB, and TrkC in DRG neurons at E16.5 (Figure 6G–6I). This absence of Trk receptor expression in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos provides a likely explanation for the lack of responsiveness of these neurons to the addition of neurotrophic factors."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T6493","span":{"begin":40,"end":52},"obj":"PR_EXT:000021998"},{"id":"T6494","span":{"begin":40,"end":70},"obj":"GO:0038179"},{"id":"T6495","span":{"begin":71,"end":74},"obj":"UBERON:0000044"},{"id":"T6496","span":{"begin":75,"end":82},"obj":"CL:0000540"},{"id":"T6497","span":{"begin":88,"end":91},"obj":"PR_EXT:000010173"},{"id":"T6498","span":{"begin":91,"end":94},"obj":"PR_EXT:000007243"},{"id":"T6499","span":{"begin":95,"end":99},"obj":"PR_EXT:000007227"},{"id":"T6500","span":{"begin":100,"end":101},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T6501","span":{"begin":102,"end":106},"obj":"PR_EXT:000009116"},{"id":"T6502","span":{"begin":110,"end":111},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T6503","span":{"begin":112,"end":119},"obj":"UBERON:0000922"},{"id":"T6504","span":{"begin":159,"end":172},"obj":"PR_EXT:000021998"},{"id":"T6505","span":{"begin":189,"end":199},"obj":"GO:0010467"},{"id":"T6506","span":{"begin":203,"end":215},"obj":"PR_EXT:000021998"},{"id":"T6507","span":{"begin":203,"end":225},"obj":"GO_EXT:0005030"},{"id":"T6508","span":{"begin":229,"end":232},"obj":"PR_EXT:000010173"},{"id":"T6509","span":{"begin":232,"end":235},"obj":"PR_EXT:000007243"},{"id":"T6510","span":{"begin":236,"end":240},"obj":"PR_EXT:000007227"},{"id":"T6511","span":{"begin":241,"end":242},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T6512","span":{"begin":243,"end":247},"obj":"PR_EXT:000009116"},{"id":"T6513","span":{"begin":251,"end":252},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T6514","span":{"begin":253,"end":260},"obj":"UBERON:0000922"},{"id":"T6515","span":{"begin":281,"end":291},"obj":"GO:0010467"},{"id":"T6516","span":{"begin":299,"end":311},"obj":"PR_EXT:000021998"},{"id":"T6517","span":{"begin":299,"end":321},"obj":"GO_EXT:0005030"},{"id":"T6518","span":{"begin":322,"end":326},"obj":"PR_EXT:000011469"},{"id":"T6519","span":{"begin":328,"end":332},"obj":"PR_EXT:000011470"},{"id":"T6520","span":{"begin":338,"end":342},"obj":"PR_EXT:000011471"},{"id":"T6521","span":{"begin":343,"end":348},"obj":"CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process"},{"id":"T6522","span":{"begin":371,"end":378},"obj":"GO_EXT:detection_or_sensing_of_stimulus"},{"id":"T6523","span":{"begin":393,"end":396},"obj":"UBERON:0000044"},{"id":"T6524","span":{"begin":400,"end":409},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T6525","span":{"begin":410,"end":417},"obj":"UBERON:0000922"},{"id":"T6526","span":{"begin":441,"end":444},"obj":"PR_EXT:000010173"},{"id":"T6527","span":{"begin":444,"end":447},"obj":"PR_EXT:000007243"},{"id":"T6528","span":{"begin":448,"end":452},"obj":"PR_EXT:000007227"},{"id":"T6529","span":{"begin":453,"end":454},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T6530","span":{"begin":455,"end":459},"obj":"PR_EXT:000009116"},{"id":"T6531","span":{"begin":463,"end":464},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T6532","span":{"begin":465,"end":472},"obj":"UBERON:0000922"},{"id":"T6533","span":{"begin":500,"end":510},"obj":"GO:0010467"},{"id":"T6534","span":{"begin":514,"end":518},"obj":"PR_EXT:000011469"},{"id":"T6535","span":{"begin":520,"end":524},"obj":"PR_EXT:000011470"},{"id":"T6536","span":{"begin":530,"end":534},"obj":"PR_EXT:000011471"},{"id":"T6537","span":{"begin":538,"end":541},"obj":"UBERON:0000044"},{"id":"T6538","span":{"begin":542,"end":549},"obj":"CL:0000540"},{"id":"T6539","span":{"begin":591,"end":603},"obj":"PR_EXT:000021997"},{"id":"T6540","span":{"begin":595,"end":603},"obj":"GO_EXT:0004872"},{"id":"T6541","span":{"begin":604,"end":614},"obj":"GO:0010467"},{"id":"T6542","span":{"begin":618,"end":621},"obj":"UBERON:0000044"},{"id":"T6543","span":{"begin":625,"end":628},"obj":"PR_EXT:000010173"},{"id":"T6544","span":{"begin":628,"end":631},"obj":"PR_EXT:000007243"},{"id":"T6545","span":{"begin":632,"end":636},"obj":"PR_EXT:000007227"},{"id":"T6546","span":{"begin":637,"end":638},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T6547","span":{"begin":639,"end":643},"obj":"PR_EXT:000009116"},{"id":"T6548","span":{"begin":647,"end":648},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T6549","span":{"begin":649,"end":656},"obj":"UBERON:0000922"},{"id":"T6550","span":{"begin":727,"end":734},"obj":"CL:0000540"},{"id":"T6551","span":{"begin":754,"end":766},"obj":"GO_EXT:neuron_development_or_generation"}],"text":"To begin to assess at which step of the neurotrophin signaling cascade DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos become unresponsive to the addition of neurotrophins, we assayed the expression of neurotrophin receptors in TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure 6). Whereas expression of the neurotrophin receptors TrkA, TrkB, and TrkC marks afferents of distinct sensory modalities in DRG of wild-type embryos (Figure 6A–6C) [4,29], TauEWS-Pea3/+ Isl1Cre/+ embryos showed complete absence of expression of TrkA, TrkB, and TrkC in DRG neurons at E16.5 (Figure 6G–6I). This absence of Trk receptor expression in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos provides a likely explanation for the lack of responsiveness of these neurons to the addition of neurotrophic factors."}

{"project":"2_test","denotations":[{"id":"15836427-11520916-84719640","span":{"begin":434,"end":435},"obj":"11520916"},{"id":"15836427-12676795-84719641","span":{"begin":436,"end":438},"obj":"12676795"},{"id":"T12912","span":{"begin":434,"end":435},"obj":"11520916"},{"id":"T52266","span":{"begin":436,"end":438},"obj":"12676795"}],"text":"To begin to assess at which step of the neurotrophin signaling cascade DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos become unresponsive to the addition of neurotrophins, we assayed the expression of neurotrophin receptors in TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure 6). Whereas expression of the neurotrophin receptors TrkA, TrkB, and TrkC marks afferents of distinct sensory modalities in DRG of wild-type embryos (Figure 6A–6C) [4,29], TauEWS-Pea3/+ Isl1Cre/+ embryos showed complete absence of expression of TrkA, TrkB, and TrkC in DRG neurons at E16.5 (Figure 6G–6I). This absence of Trk receptor expression in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos provides a likely explanation for the lack of responsiveness of these neurons to the addition of neurotrophic factors."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T6203","span":{"begin":40,"end":52},"obj":"PR:000021998"},{"id":"T6204","span":{"begin":40,"end":70},"obj":"GO:0038179"},{"id":"T6205","span":{"begin":71,"end":74},"obj":"UBERON:0000044"},{"id":"T6206","span":{"begin":75,"end":82},"obj":"CL:0000540"},{"id":"T6207","span":{"begin":88,"end":91},"obj":"PR:000010173"},{"id":"T6208","span":{"begin":91,"end":94},"obj":"PR:000007243"},{"id":"T6209","span":{"begin":95,"end":99},"obj":"PR:000007227"},{"id":"T6210","span":{"begin":102,"end":106},"obj":"PR:000009116"},{"id":"T6211","span":{"begin":112,"end":119},"obj":"UBERON:0000922"},{"id":"T6212","span":{"begin":159,"end":172},"obj":"PR:000021998"},{"id":"T6213","span":{"begin":189,"end":199},"obj":"GO:0010467"},{"id":"T6214","span":{"begin":203,"end":215},"obj":"PR:000021998"},{"id":"T6215","span":{"begin":229,"end":232},"obj":"PR:000010173"},{"id":"T6216","span":{"begin":232,"end":235},"obj":"PR:000007243"},{"id":"T6217","span":{"begin":236,"end":240},"obj":"PR:000007227"},{"id":"T6218","span":{"begin":243,"end":247},"obj":"PR:000009116"},{"id":"T6219","span":{"begin":253,"end":260},"obj":"UBERON:0000922"},{"id":"T6220","span":{"begin":281,"end":291},"obj":"GO:0010467"},{"id":"T6221","span":{"begin":299,"end":311},"obj":"PR:000021998"},{"id":"T6222","span":{"begin":322,"end":326},"obj":"PR:000011469"},{"id":"T6223","span":{"begin":328,"end":332},"obj":"PR:000011470"},{"id":"T6224","span":{"begin":338,"end":342},"obj":"PR:000011471"},{"id":"T6225","span":{"begin":393,"end":396},"obj":"UBERON:0000044"},{"id":"T6226","span":{"begin":410,"end":417},"obj":"UBERON:0000922"},{"id":"T6227","span":{"begin":441,"end":444},"obj":"PR:000010173"},{"id":"T6228","span":{"begin":444,"end":447},"obj":"PR:000007243"},{"id":"T6229","span":{"begin":448,"end":452},"obj":"PR:000007227"},{"id":"T6230","span":{"begin":455,"end":459},"obj":"PR:000009116"},{"id":"T6231","span":{"begin":465,"end":472},"obj":"UBERON:0000922"},{"id":"T6232","span":{"begin":500,"end":510},"obj":"GO:0010467"},{"id":"T6233","span":{"begin":514,"end":518},"obj":"PR:000011469"},{"id":"T6234","span":{"begin":520,"end":524},"obj":"PR:000011470"},{"id":"T6235","span":{"begin":530,"end":534},"obj":"PR:000011471"},{"id":"T6236","span":{"begin":538,"end":541},"obj":"UBERON:0000044"},{"id":"T6237","span":{"begin":542,"end":549},"obj":"CL:0000540"},{"id":"T6238","span":{"begin":591,"end":603},"obj":"PR:000021997"},{"id":"T6239","span":{"begin":604,"end":614},"obj":"GO:0010467"},{"id":"T6240","span":{"begin":618,"end":621},"obj":"UBERON:0000044"},{"id":"T6241","span":{"begin":625,"end":628},"obj":"PR:000010173"},{"id":"T6242","span":{"begin":628,"end":631},"obj":"PR:000007243"},{"id":"T6243","span":{"begin":632,"end":636},"obj":"PR:000007227"},{"id":"T6244","span":{"begin":639,"end":643},"obj":"PR:000009116"},{"id":"T6245","span":{"begin":649,"end":656},"obj":"UBERON:0000922"},{"id":"T6246","span":{"begin":727,"end":734},"obj":"CL:0000540"}],"text":"To begin to assess at which step of the neurotrophin signaling cascade DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos become unresponsive to the addition of neurotrophins, we assayed the expression of neurotrophin receptors in TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure 6). Whereas expression of the neurotrophin receptors TrkA, TrkB, and TrkC marks afferents of distinct sensory modalities in DRG of wild-type embryos (Figure 6A–6C) [4,29], TauEWS-Pea3/+ Isl1Cre/+ embryos showed complete absence of expression of TrkA, TrkB, and TrkC in DRG neurons at E16.5 (Figure 6G–6I). This absence of Trk receptor expression in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos provides a likely explanation for the lack of responsiveness of these neurons to the addition of neurotrophic factors."}