PMC:1084331 / 21870-22763 JSONTXT

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    craft-sa-dev

    To determine whether neuronal survival of DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos in the absence of neurotrophic support is sufficient to explain the observed neuronal outgrowth, we analyzed DRG isolated from mice mutant in the proapoptotic gene Bax [27]. Consistent with previous results, Bax/ DRG neurons survived without neurotrophic support [28]. In contrast, neurite outgrowth of Bax/ DRG neurons was significantly less (see Figure 4G) than that of either DRG from TauEWS-Pea3/+ Isl1Cre/+ embryos cultured in the absence of neurotrophic support (see Figure 4D) or Bax/ DRG neurons cultured in the presence of neurotrophic support (see Figure 4H and 4I). These findings suggest that in addition to mediating neurotrophin-independent neuronal survival, expression of EWS-Pea3 in early post-mitotic neurons also promotes neurite outgrowth in a neurotrophin-independent manner.

    craft-ca-core-ex-dev

    To determine whether neuronal survival of DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos in the absence of neurotrophic support is sufficient to explain the observed neuronal outgrowth, we analyzed DRG isolated from mice mutant in the proapoptotic gene Bax [27]. Consistent with previous results, Bax/ DRG neurons survived without neurotrophic support [28]. In contrast, neurite outgrowth of Bax/ DRG neurons was significantly less (see Figure 4G) than that of either DRG from TauEWS-Pea3/+ Isl1Cre/+ embryos cultured in the absence of neurotrophic support (see Figure 4D) or Bax/ DRG neurons cultured in the presence of neurotrophic support (see Figure 4H and 4I). These findings suggest that in addition to mediating neurotrophin-independent neuronal survival, expression of EWS-Pea3 in early post-mitotic neurons also promotes neurite outgrowth in a neurotrophin-independent manner.

    2_test

    To determine whether neuronal survival of DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos in the absence of neurotrophic support is sufficient to explain the observed neuronal outgrowth, we analyzed DRG isolated from mice mutant in the proapoptotic gene Bax [27]. Consistent with previous results, Bax−/− DRG neurons survived without neurotrophic support [28]. In contrast, neurite outgrowth of Bax−/− DRG neurons was significantly less (see Figure 4G) than that of either DRG from TauEWS-Pea3/+ Isl1Cre/+ embryos cultured in the absence of neurotrophic support (see Figure 4D) or Bax−/− DRG neurons cultured in the presence of neurotrophic support (see Figure 4H and 4I). These findings suggest that in addition to mediating neurotrophin-independent neuronal survival, expression of EWS-Pea3 in early post-mitotic neurons also promotes neurite outgrowth in a neurotrophin-independent manner.

    craft-ca-core-dev

    To determine whether neuronal survival of DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos in the absence of neurotrophic support is sufficient to explain the observed neuronal outgrowth, we analyzed DRG isolated from mice mutant in the proapoptotic gene Bax [27]. Consistent with previous results, Bax−/− DRG neurons survived without neurotrophic support [28]. In contrast, neurite outgrowth of Bax−/− DRG neurons was significantly less (see Figure 4G) than that of either DRG from TauEWS-Pea3/+ Isl1Cre/+ embryos cultured in the absence of neurotrophic support (see Figure 4D) or Bax−/− DRG neurons cultured in the presence of neurotrophic support (see Figure 4H and 4I). These findings suggest that in addition to mediating neurotrophin-independent neuronal survival, expression of EWS-Pea3 in early post-mitotic neurons also promotes neurite outgrowth in a neurotrophin-independent manner.