Id |
Subject |
Object |
Predicate |
Lexical cue |
T261 |
16-22 |
NN |
denotes |
Switch |
T262 |
0-91 |
sentence |
denotes |
A Developmental Switch in the Response of DRG Neurons to ETS Transcription Factor Signaling |
T263 |
2-15 |
JJ |
denotes |
Developmental |
T264 |
23-25 |
IN |
denotes |
in |
T265 |
26-29 |
DT |
denotes |
the |
T266 |
30-38 |
NN |
denotes |
Response |
T267 |
39-41 |
IN |
denotes |
of |
T268 |
42-45 |
NN |
denotes |
DRG |
T269 |
46-53 |
NNS |
denotes |
Neurons |
T270 |
54-56 |
IN |
denotes |
to |
T271 |
57-60 |
NN |
denotes |
ETS |
T272 |
82-91 |
NN |
denotes |
Signaling |
T273 |
61-74 |
NN |
denotes |
Transcription |
T274 |
75-81 |
NN |
denotes |
Factor |
T275 |
91-92 |
sentence |
denotes |
|
T276 |
152-323 |
sentence |
denotes |
Two ETS transcription factors of the Pea3 subfamily are induced in subpopulations of dorsal root ganglion (DRG) sensory and spinal motor neurons by target-derived factors. |
T277 |
152-155 |
CD |
denotes |
Two |
T278 |
174-181 |
NNS |
denotes |
factors |
T279 |
156-159 |
NN |
denotes |
ETS |
T280 |
160-173 |
NN |
denotes |
transcription |
T281 |
208-215 |
VBN |
denotes |
induced |
T282 |
182-184 |
IN |
denotes |
of |
T283 |
185-188 |
DT |
denotes |
the |
T284 |
194-203 |
NN |
denotes |
subfamily |
T285 |
189-193 |
NN |
denotes |
Pea3 |
T286 |
204-207 |
VBP |
denotes |
are |
T287 |
216-218 |
IN |
denotes |
in |
T288 |
219-233 |
NNS |
denotes |
subpopulations |
T289 |
234-236 |
IN |
denotes |
of |
T290 |
237-243 |
JJ |
denotes |
dorsal |
T291 |
249-257 |
NN |
denotes |
ganglion |
T292 |
244-248 |
NN |
denotes |
root |
T293 |
289-296 |
NNS |
denotes |
neurons |
T294 |
258-259 |
-LRB- |
denotes |
( |
T295 |
259-262 |
NN |
denotes |
DRG |
T296 |
262-263 |
-RRB- |
denotes |
) |
T297 |
264-271 |
JJ |
denotes |
sensory |
T298 |
272-275 |
CC |
denotes |
and |
T299 |
276-282 |
JJ |
denotes |
spinal |
T300 |
283-288 |
NN |
denotes |
motor |
T301 |
297-299 |
IN |
denotes |
by |
T302 |
300-306 |
NN |
denotes |
target |
T303 |
307-314 |
VBN |
denotes |
derived |
T304 |
306-307 |
HYPH |
denotes |
- |
T305 |
315-322 |
NNS |
denotes |
factors |
T306 |
322-323 |
. |
denotes |
. |
T307 |
323-429 |
sentence |
denotes |
Their expression controls late aspects of neuronal differentiation such as target invasion and branching. |
T308 |
324-329 |
PRP$ |
denotes |
Their |
T309 |
330-340 |
NN |
denotes |
expression |
T310 |
341-349 |
VBZ |
denotes |
controls |
T311 |
350-354 |
JJ |
denotes |
late |
T312 |
355-362 |
NNS |
denotes |
aspects |
T313 |
363-365 |
IN |
denotes |
of |
T314 |
366-374 |
JJ |
denotes |
neuronal |
T315 |
375-390 |
NN |
denotes |
differentiation |
T316 |
391-395 |
JJ |
denotes |
such |
T317 |
396-398 |
IN |
denotes |
as |
T318 |
399-405 |
NN |
denotes |
target |
T319 |
406-414 |
NN |
denotes |
invasion |
T320 |
415-418 |
CC |
denotes |
and |
T321 |
419-428 |
NN |
denotes |
branching |
T322 |
428-429 |
. |
denotes |
. |
T323 |
429-557 |
sentence |
denotes |
Here, we show that the late onset of ETS gene expression is an essential requirement for normal sensory neuron differentiation. |
T324 |
430-434 |
RB |
denotes |
Here |
T325 |
439-443 |
VBP |
denotes |
show |
T326 |
434-436 |
, |
denotes |
, |
T327 |
436-438 |
PRP |
denotes |
we |
T328 |
444-448 |
IN |
denotes |
that |
T329 |
487-489 |
VBZ |
denotes |
is |
T330 |
449-452 |
DT |
denotes |
the |
T331 |
458-463 |
NN |
denotes |
onset |
T332 |
453-457 |
JJ |
denotes |
late |
T333 |
464-466 |
IN |
denotes |
of |
T334 |
467-470 |
NN |
denotes |
ETS |
T335 |
471-475 |
NN |
denotes |
gene |
T336 |
476-486 |
NN |
denotes |
expression |
T337 |
490-492 |
DT |
denotes |
an |
T338 |
503-514 |
NN |
denotes |
requirement |
T339 |
493-502 |
JJ |
denotes |
essential |
T340 |
515-518 |
IN |
denotes |
for |
T341 |
519-525 |
JJ |
denotes |
normal |
T342 |
541-556 |
NN |
denotes |
differentiation |
T343 |
526-533 |
JJ |
denotes |
sensory |
T344 |
534-540 |
NN |
denotes |
neuron |
T345 |
556-557 |
. |
denotes |
. |
T346 |
557-780 |
sentence |
denotes |
We provide genetic evidence in the mouse that precocious ETS expression in DRG sensory neurons perturbs axonal projections, the acquisition of terminal differentiation markers, and their dependence on neurotrophic support. |
T347 |
558-560 |
PRP |
denotes |
We |
T348 |
561-568 |
VBP |
denotes |
provide |
T349 |
569-576 |
JJ |
denotes |
genetic |
T350 |
577-585 |
NN |
denotes |
evidence |
T351 |
586-588 |
IN |
denotes |
in |
T352 |
589-592 |
DT |
denotes |
the |
T353 |
593-598 |
NN |
denotes |
mouse |
T354 |
599-603 |
IN |
denotes |
that |
T355 |
653-661 |
VBZ |
denotes |
perturbs |
T356 |
604-614 |
JJ |
denotes |
precocious |
T357 |
619-629 |
NN |
denotes |
expression |
T358 |
615-618 |
NN |
denotes |
ETS |
T359 |
630-632 |
IN |
denotes |
in |
T360 |
633-636 |
NN |
denotes |
DRG |
T361 |
645-652 |
NNS |
denotes |
neurons |
T362 |
637-644 |
JJ |
denotes |
sensory |
T363 |
662-668 |
JJ |
denotes |
axonal |
T364 |
669-680 |
NNS |
denotes |
projections |
T365 |
680-682 |
, |
denotes |
, |
T366 |
682-685 |
DT |
denotes |
the |
T367 |
686-697 |
NN |
denotes |
acquisition |
T368 |
698-700 |
IN |
denotes |
of |
T369 |
701-709 |
JJ |
denotes |
terminal |
T370 |
726-733 |
NNS |
denotes |
markers |
T371 |
710-725 |
NN |
denotes |
differentiation |
T372 |
733-735 |
, |
denotes |
, |
T373 |
735-738 |
CC |
denotes |
and |
T374 |
739-744 |
PRP$ |
denotes |
their |
T375 |
745-755 |
NN |
denotes |
dependence |
T376 |
756-758 |
IN |
denotes |
on |
T377 |
759-771 |
JJ |
denotes |
neurotrophic |
T378 |
772-779 |
NN |
denotes |
support |
T379 |
779-780 |
. |
denotes |
. |
T380 |
780-1002 |
sentence |
denotes |
Together, our findings indicate that DRG sensory neurons exhibit a temporal developmental switch that can be revealed by distinct responses to ETS transcription factor signaling at sequential steps of neuronal maturation. |
T381 |
781-789 |
RB |
denotes |
Together |
T382 |
804-812 |
VBP |
denotes |
indicate |
T383 |
789-791 |
, |
denotes |
, |
T384 |
791-794 |
PRP$ |
denotes |
our |
T385 |
795-803 |
NNS |
denotes |
findings |
T386 |
813-817 |
IN |
denotes |
that |
T387 |
838-845 |
VBP |
denotes |
exhibit |
T388 |
818-821 |
NN |
denotes |
DRG |
T389 |
830-837 |
NNS |
denotes |
neurons |
T390 |
822-829 |
JJ |
denotes |
sensory |
T391 |
846-847 |
DT |
denotes |
a |
T392 |
871-877 |
NN |
denotes |
switch |
T393 |
848-856 |
JJ |
denotes |
temporal |
T394 |
857-870 |
JJ |
denotes |
developmental |
T395 |
878-882 |
WDT |
denotes |
that |
T396 |
890-898 |
VBN |
denotes |
revealed |
T397 |
883-886 |
MD |
denotes |
can |
T398 |
887-889 |
VB |
denotes |
be |
T399 |
899-901 |
IN |
denotes |
by |
T400 |
902-910 |
JJ |
denotes |
distinct |
T401 |
911-920 |
NNS |
denotes |
responses |
T402 |
921-923 |
IN |
denotes |
to |
T403 |
924-927 |
NN |
denotes |
ETS |
T404 |
949-958 |
NN |
denotes |
signaling |
T405 |
928-941 |
NN |
denotes |
transcription |
T406 |
942-948 |
NN |
denotes |
factor |
T407 |
959-961 |
IN |
denotes |
at |
T408 |
962-972 |
JJ |
denotes |
sequential |
T409 |
973-978 |
NNS |
denotes |
steps |
T410 |
979-981 |
IN |
denotes |
of |
T411 |
982-990 |
JJ |
denotes |
neuronal |
T412 |
991-1001 |
NN |
denotes |
maturation |
T413 |
1001-1002 |
. |
denotes |
. |
T1510 |
1235-1243 |
JJ |
denotes |
Neuronal |
T1511 |
1244-1259 |
NN |
denotes |
differentiation |
T1512 |
1260-1262 |
VBZ |
denotes |
is |
T1513 |
1263-1264 |
DT |
denotes |
a |
T1514 |
1276-1283 |
NN |
denotes |
process |
T1515 |
1265-1275 |
JJ |
denotes |
protracted |
T1516 |
1284-1290 |
IN |
denotes |
during |
T1517 |
1321-1328 |
VBP |
denotes |
express |
T1518 |
1291-1296 |
WDT |
denotes |
which |
T1519 |
1297-1302 |
RB |
denotes |
newly |
T1520 |
1303-1312 |
VBN |
denotes |
generated |
T1521 |
1313-1320 |
NNS |
denotes |
neurons |
T1522 |
1329-1337 |
JJ |
denotes |
distinct |
T1523 |
1361-1369 |
NNS |
denotes |
programs |
T1524 |
1338-1346 |
JJ |
denotes |
cellular |
T1525 |
1347-1350 |
CC |
denotes |
and |
T1526 |
1351-1360 |
JJ |
denotes |
molecular |
T1527 |
1370-1372 |
IN |
denotes |
at |
T1528 |
1373-1380 |
JJ |
denotes |
precise |
T1529 |
1381-1386 |
NNS |
denotes |
times |
T1530 |
1387-1393 |
IN |
denotes |
during |
T1531 |
1394-1399 |
PRP$ |
denotes |
their |
T1532 |
1400-1410 |
NN |
denotes |
maturation |
T1533 |
1410-1412 |
: |
denotes |
: |
T1534 |
1412-1416 |
JJ |
denotes |
long |
T1535 |
1417-1425 |
NN |
denotes |
distance |
T1536 |
1416-1417 |
HYPH |
denotes |
- |
T1537 |
1431-1440 |
NN |
denotes |
outgrowth |
T1538 |
1426-1430 |
NN |
denotes |
axon |
T1539 |
1440-1442 |
, |
denotes |
, |
T1540 |
1442-1452 |
JJ |
denotes |
subsequent |
T1541 |
1462-1471 |
NN |
denotes |
branching |
T1542 |
1453-1461 |
JJ |
denotes |
terminal |
T1543 |
1471-1473 |
, |
denotes |
, |
T1544 |
1473-1476 |
CC |
denotes |
and |
T1545 |
1477-1484 |
RB |
denotes |
finally |
T1546 |
1485-1499 |
NN |
denotes |
synaptogenesis |
T1547 |
1499-1500 |
. |
denotes |
. |
T1548 |
1500-1716 |
sentence |
denotes |
Many important aspects of neuronal character appear to be acquired through the expression of transcription factors at progenitor cell stages, whereas others depend on expression immediately upon cell cycle exit [1]. |
T1549 |
1501-1505 |
JJ |
denotes |
Many |
T1550 |
1516-1523 |
NNS |
denotes |
aspects |
T1551 |
1506-1515 |
JJ |
denotes |
important |
T1552 |
1546-1552 |
VBP |
denotes |
appear |
T1553 |
1524-1526 |
IN |
denotes |
of |
T1554 |
1527-1535 |
JJ |
denotes |
neuronal |
T1555 |
1536-1545 |
NN |
denotes |
character |
T1556 |
1553-1555 |
TO |
denotes |
to |
T1557 |
1559-1567 |
VBN |
denotes |
acquired |
T1558 |
1556-1558 |
VB |
denotes |
be |
T1559 |
1568-1575 |
IN |
denotes |
through |
T1560 |
1576-1579 |
DT |
denotes |
the |
T1561 |
1580-1590 |
NN |
denotes |
expression |
T1562 |
1591-1593 |
IN |
denotes |
of |
T1563 |
1594-1607 |
NN |
denotes |
transcription |
T1564 |
1608-1615 |
NNS |
denotes |
factors |
T1565 |
1616-1618 |
IN |
denotes |
at |
T1566 |
1619-1629 |
NN |
denotes |
progenitor |
T1567 |
1635-1641 |
NNS |
denotes |
stages |
T1568 |
1630-1634 |
NN |
denotes |
cell |
T1569 |
1641-1643 |
, |
denotes |
, |
T1570 |
1643-1650 |
IN |
denotes |
whereas |
T1571 |
1658-1664 |
VBP |
denotes |
depend |
T1572 |
1651-1657 |
NNS |
denotes |
others |
T1573 |
1665-1667 |
IN |
denotes |
on |
T1574 |
1668-1678 |
NN |
denotes |
expression |
T1575 |
1679-1690 |
RB |
denotes |
immediately |
T1576 |
1691-1695 |
IN |
denotes |
upon |
T1577 |
1696-1700 |
NN |
denotes |
cell |
T1578 |
1701-1706 |
NN |
denotes |
cycle |
T1579 |
1707-1711 |
NN |
denotes |
exit |
T1580 |
1712-1713 |
-LRB- |
denotes |
[ |
T1581 |
1713-1714 |
CD |
denotes |
1 |
T1582 |
1714-1715 |
-RRB- |
denotes |
] |
T1583 |
1715-1716 |
. |
denotes |
. |
T1584 |
1716-1969 |
sentence |
denotes |
But whether the orderly expression and activity of transcriptional programs at much later developmental stages, well after cell cycle exit, is an essential step in the progression of neuronal differentiation and circuit assembly has yet to be resolved. |
T1585 |
1717-1720 |
CC |
denotes |
But |
T1586 |
1946-1949 |
VBZ |
denotes |
has |
T1587 |
1721-1728 |
IN |
denotes |
whether |
T1588 |
1857-1859 |
VBZ |
denotes |
is |
T1589 |
1729-1732 |
DT |
denotes |
the |
T1590 |
1741-1751 |
NN |
denotes |
expression |
T1591 |
1733-1740 |
JJ |
denotes |
orderly |
T1592 |
1752-1755 |
CC |
denotes |
and |
T1593 |
1756-1764 |
NN |
denotes |
activity |
T1594 |
1765-1767 |
IN |
denotes |
of |
T1595 |
1768-1783 |
JJ |
denotes |
transcriptional |
T1596 |
1784-1792 |
NNS |
denotes |
programs |
T1597 |
1793-1795 |
IN |
denotes |
at |
T1598 |
1796-1800 |
RB |
denotes |
much |
T1599 |
1801-1806 |
JJ |
denotes |
later |
T1600 |
1821-1827 |
NNS |
denotes |
stages |
T1601 |
1807-1820 |
JJ |
denotes |
developmental |
T1602 |
1827-1829 |
, |
denotes |
, |
T1603 |
1829-1833 |
RB |
denotes |
well |
T1604 |
1834-1839 |
IN |
denotes |
after |
T1605 |
1840-1844 |
NN |
denotes |
cell |
T1606 |
1845-1850 |
NN |
denotes |
cycle |
T1607 |
1851-1855 |
NN |
denotes |
exit |
T1608 |
1855-1857 |
, |
denotes |
, |
T1609 |
1860-1862 |
DT |
denotes |
an |
T1610 |
1873-1877 |
NN |
denotes |
step |
T1611 |
1863-1872 |
JJ |
denotes |
essential |
T1612 |
1878-1880 |
IN |
denotes |
in |
T1613 |
1881-1884 |
DT |
denotes |
the |
T1614 |
1885-1896 |
NN |
denotes |
progression |
T1615 |
1897-1899 |
IN |
denotes |
of |
T1616 |
1900-1908 |
JJ |
denotes |
neuronal |
T1617 |
1909-1924 |
NN |
denotes |
differentiation |
T1618 |
1925-1928 |
CC |
denotes |
and |
T1619 |
1929-1936 |
NN |
denotes |
circuit |
T1620 |
1937-1945 |
NN |
denotes |
assembly |
T1621 |
1950-1953 |
RB |
denotes |
yet |
T1622 |
1954-1956 |
TO |
denotes |
to |
T1623 |
1960-1968 |
VBN |
denotes |
resolved |
T1624 |
1957-1959 |
VB |
denotes |
be |
T1625 |
1968-1969 |
. |
denotes |
. |
T1626 |
1969-2245 |
sentence |
denotes |
The differentiation of sensory neurons of dorsal root ganglia (DRG) has been studied extensively with respect to inductive events that specify neuronal fate [2,3], as well as the involvement of late target-derived neurotrophic factors in the control of neuronal survival [4]. |
T1627 |
1970-1973 |
DT |
denotes |
The |
T1628 |
1974-1989 |
NN |
denotes |
differentiation |
T1629 |
2047-2054 |
VBN |
denotes |
studied |
T1630 |
1990-1992 |
IN |
denotes |
of |
T1631 |
1993-2000 |
JJ |
denotes |
sensory |
T1632 |
2001-2008 |
NNS |
denotes |
neurons |
T1633 |
2009-2011 |
IN |
denotes |
of |
T1634 |
2012-2018 |
JJ |
denotes |
dorsal |
T1635 |
2024-2031 |
NNS |
denotes |
ganglia |
T1636 |
2019-2023 |
NN |
denotes |
root |
T1637 |
2032-2033 |
-LRB- |
denotes |
( |
T1638 |
2033-2036 |
NN |
denotes |
DRG |
T1639 |
2036-2037 |
-RRB- |
denotes |
) |
T1640 |
2038-2041 |
VBZ |
denotes |
has |
T1641 |
2042-2046 |
VBN |
denotes |
been |
T1642 |
2055-2066 |
RB |
denotes |
extensively |
T1643 |
2067-2071 |
IN |
denotes |
with |
T1644 |
2072-2079 |
NN |
denotes |
respect |
T1645 |
2080-2082 |
IN |
denotes |
to |
T1646 |
2083-2092 |
JJ |
denotes |
inductive |
T1647 |
2093-2099 |
NNS |
denotes |
events |
T1648 |
2100-2104 |
WDT |
denotes |
that |
T1649 |
2105-2112 |
VBP |
denotes |
specify |
T1650 |
2113-2121 |
JJ |
denotes |
neuronal |
T1651 |
2122-2126 |
NN |
denotes |
fate |
T1652 |
2127-2128 |
-LRB- |
denotes |
[ |
T1653 |
2130-2131 |
CD |
denotes |
3 |
T1654 |
2128-2129 |
CD |
denotes |
2 |
T1655 |
2129-2130 |
, |
denotes |
, |
T1656 |
2131-2132 |
-RRB- |
denotes |
] |
T1657 |
2132-2134 |
, |
denotes |
, |
T1658 |
2134-2136 |
RB |
denotes |
as |
T1659 |
2142-2144 |
IN |
denotes |
as |
T1660 |
2137-2141 |
RB |
denotes |
well |
T1661 |
2145-2148 |
DT |
denotes |
the |
T1662 |
2149-2160 |
NN |
denotes |
involvement |
T1663 |
2161-2163 |
IN |
denotes |
of |
T1664 |
2164-2168 |
JJ |
denotes |
late |
T1665 |
2197-2204 |
NNS |
denotes |
factors |
T1666 |
2169-2175 |
NN |
denotes |
target |
T1667 |
2176-2183 |
VBN |
denotes |
derived |
T1668 |
2175-2176 |
HYPH |
denotes |
- |
T1669 |
2184-2196 |
JJ |
denotes |
neurotrophic |
T1670 |
2205-2207 |
IN |
denotes |
in |
T1671 |
2208-2211 |
DT |
denotes |
the |
T1672 |
2212-2219 |
NN |
denotes |
control |
T1673 |
2220-2222 |
IN |
denotes |
of |
T1674 |
2223-2231 |
JJ |
denotes |
neuronal |
T1675 |
2232-2240 |
NN |
denotes |
survival |
T1676 |
2241-2242 |
-LRB- |
denotes |
[ |
T1677 |
2242-2243 |
CD |
denotes |
4 |
T1678 |
2243-2244 |
-RRB- |
denotes |
] |
T1679 |
2244-2245 |
. |
denotes |
. |
T1680 |
2245-2392 |
sentence |
denotes |
Recent evidence has begun to emerge that target-derived factors are also involved in regulating later aspects of neuronal differentiation [5,6,7]. |
T1681 |
2246-2252 |
JJ |
denotes |
Recent |
T1682 |
2253-2261 |
NN |
denotes |
evidence |
T1683 |
2266-2271 |
VBN |
denotes |
begun |
T1684 |
2262-2265 |
VBZ |
denotes |
has |
T1685 |
2272-2274 |
TO |
denotes |
to |
T1686 |
2275-2281 |
VB |
denotes |
emerge |
T1687 |
2282-2286 |
IN |
denotes |
that |
T1688 |
2319-2327 |
VBN |
denotes |
involved |
T1689 |
2287-2293 |
NN |
denotes |
target |
T1690 |
2294-2301 |
VBN |
denotes |
derived |
T1691 |
2293-2294 |
HYPH |
denotes |
- |
T1692 |
2302-2309 |
NNS |
denotes |
factors |
T1693 |
2310-2313 |
VBP |
denotes |
are |
T1694 |
2314-2318 |
RB |
denotes |
also |
T1695 |
2328-2330 |
IN |
denotes |
in |
T1696 |
2331-2341 |
VBG |
denotes |
regulating |
T1697 |
2342-2347 |
JJ |
denotes |
later |
T1698 |
2348-2355 |
NNS |
denotes |
aspects |
T1699 |
2356-2358 |
IN |
denotes |
of |
T1700 |
2359-2367 |
JJ |
denotes |
neuronal |
T1701 |
2368-2383 |
NN |
denotes |
differentiation |
T1702 |
2384-2385 |
-LRB- |
denotes |
[ |
T1703 |
2389-2390 |
CD |
denotes |
7 |
T1704 |
2385-2386 |
CD |
denotes |
5 |
T1705 |
2386-2387 |
, |
denotes |
, |
T1706 |
2387-2388 |
CD |
denotes |
6 |
T1707 |
2388-2389 |
, |
denotes |
, |
T1708 |
2390-2391 |
-RRB- |
denotes |
] |
T1709 |
2391-2392 |
. |
denotes |
. |
T1710 |
2392-2642 |
sentence |
denotes |
In particular, genetic experiments have addressed the survival-independent role of neurotrophic factors during development by exploiting strains of mice defective both in neurotrophin signaling and in the function of the proapoptotic gene Bax [8,9]. |
T1711 |
2393-2395 |
IN |
denotes |
In |
T1712 |
2433-2442 |
VBN |
denotes |
addressed |
T1713 |
2396-2406 |
JJ |
denotes |
particular |
T1714 |
2406-2408 |
, |
denotes |
, |
T1715 |
2408-2415 |
JJ |
denotes |
genetic |
T1716 |
2416-2427 |
NNS |
denotes |
experiments |
T1717 |
2428-2432 |
VBP |
denotes |
have |
T1718 |
2443-2446 |
DT |
denotes |
the |
T1719 |
2468-2472 |
NN |
denotes |
role |
T1720 |
2447-2455 |
NN |
denotes |
survival |
T1721 |
2456-2467 |
JJ |
denotes |
independent |
T1722 |
2455-2456 |
HYPH |
denotes |
- |
T1723 |
2473-2475 |
IN |
denotes |
of |
T1724 |
2476-2488 |
JJ |
denotes |
neurotrophic |
T1725 |
2489-2496 |
NNS |
denotes |
factors |
T1726 |
2497-2503 |
IN |
denotes |
during |
T1727 |
2504-2515 |
NN |
denotes |
development |
T1728 |
2516-2518 |
IN |
denotes |
by |
T1729 |
2519-2529 |
VBG |
denotes |
exploiting |
T1730 |
2530-2537 |
NNS |
denotes |
strains |
T1731 |
2538-2540 |
IN |
denotes |
of |
T1732 |
2541-2545 |
NNS |
denotes |
mice |
T1733 |
2546-2555 |
JJ |
denotes |
defective |
T1734 |
2556-2560 |
CC |
denotes |
both |
T1735 |
2561-2563 |
IN |
denotes |
in |
T1736 |
2564-2576 |
NN |
denotes |
neurotrophin |
T1737 |
2577-2586 |
NN |
denotes |
signaling |
T1738 |
2587-2590 |
CC |
denotes |
and |
T1739 |
2591-2593 |
IN |
denotes |
in |
T1740 |
2594-2597 |
DT |
denotes |
the |
T1741 |
2598-2606 |
NN |
denotes |
function |
T1742 |
2607-2609 |
IN |
denotes |
of |
T1743 |
2610-2613 |
DT |
denotes |
the |
T1744 |
2632-2635 |
NN |
denotes |
Bax |
T1745 |
2614-2626 |
JJ |
denotes |
proapoptotic |
T1746 |
2627-2631 |
NN |
denotes |
gene |
T1747 |
2636-2637 |
-LRB- |
denotes |
[ |
T1748 |
2639-2640 |
CD |
denotes |
9 |
T1749 |
2637-2638 |
CD |
denotes |
8 |
T1750 |
2638-2639 |
, |
denotes |
, |
T1751 |
2640-2641 |
-RRB- |
denotes |
] |
T1752 |
2641-2642 |
. |
denotes |
. |
T1753 |
2642-2831 |
sentence |
denotes |
These studies, for example, have revealed that neurotrophin signaling controls the acquisition of peptidergic traits in nociceptive DRG neurons and the control of target innervation [8,9]. |
T1754 |
2643-2648 |
DT |
denotes |
These |
T1755 |
2649-2656 |
NNS |
denotes |
studies |
T1756 |
2676-2684 |
VBN |
denotes |
revealed |
T1757 |
2656-2658 |
, |
denotes |
, |
T1758 |
2658-2661 |
IN |
denotes |
for |
T1759 |
2662-2669 |
NN |
denotes |
example |
T1760 |
2669-2671 |
, |
denotes |
, |
T1761 |
2671-2675 |
VBP |
denotes |
have |
T1762 |
2685-2689 |
IN |
denotes |
that |
T1763 |
2713-2721 |
VBZ |
denotes |
controls |
T1764 |
2690-2702 |
NN |
denotes |
neurotrophin |
T1765 |
2703-2712 |
NN |
denotes |
signaling |
T1766 |
2722-2725 |
DT |
denotes |
the |
T1767 |
2726-2737 |
NN |
denotes |
acquisition |
T1768 |
2738-2740 |
IN |
denotes |
of |
T1769 |
2741-2752 |
JJ |
denotes |
peptidergic |
T1770 |
2753-2759 |
NNS |
denotes |
traits |
T1771 |
2760-2762 |
IN |
denotes |
in |
T1772 |
2763-2774 |
JJ |
denotes |
nociceptive |
T1773 |
2779-2786 |
NNS |
denotes |
neurons |
T1774 |
2775-2778 |
NN |
denotes |
DRG |
T1775 |
2787-2790 |
CC |
denotes |
and |
T1776 |
2791-2794 |
DT |
denotes |
the |
T1777 |
2795-2802 |
NN |
denotes |
control |
T1778 |
2803-2805 |
IN |
denotes |
of |
T1779 |
2806-2812 |
NN |
denotes |
target |
T1780 |
2813-2824 |
NN |
denotes |
innervation |
T1781 |
2825-2826 |
-LRB- |
denotes |
[ |
T1782 |
2828-2829 |
CD |
denotes |
9 |
T1783 |
2826-2827 |
CD |
denotes |
8 |
T1784 |
2827-2828 |
, |
denotes |
, |
T1785 |
2829-2830 |
-RRB- |
denotes |
] |
T1786 |
2830-2831 |
. |
denotes |
. |
T1787 |
2831-2964 |
sentence |
denotes |
The onset of some transcriptional programs in neurons, however, has also been shown to occur long after neurons exit the cell cycle. |
T1788 |
2832-2835 |
DT |
denotes |
The |
T1789 |
2836-2841 |
NN |
denotes |
onset |
T1790 |
2910-2915 |
VBN |
denotes |
shown |
T1791 |
2842-2844 |
IN |
denotes |
of |
T1792 |
2845-2849 |
DT |
denotes |
some |
T1793 |
2866-2874 |
NNS |
denotes |
programs |
T1794 |
2850-2865 |
JJ |
denotes |
transcriptional |
T1795 |
2875-2877 |
IN |
denotes |
in |
T1796 |
2878-2885 |
NNS |
denotes |
neurons |
T1797 |
2885-2887 |
, |
denotes |
, |
T1798 |
2887-2894 |
RB |
denotes |
however |
T1799 |
2894-2896 |
, |
denotes |
, |
T1800 |
2896-2899 |
VBZ |
denotes |
has |
T1801 |
2900-2904 |
RB |
denotes |
also |
T1802 |
2905-2909 |
VBN |
denotes |
been |
T1803 |
2916-2918 |
TO |
denotes |
to |
T1804 |
2919-2924 |
VB |
denotes |
occur |
T1805 |
2925-2929 |
RB |
denotes |
long |
T1806 |
2944-2948 |
VBP |
denotes |
exit |
T1807 |
2930-2935 |
IN |
denotes |
after |
T1808 |
2936-2943 |
NNS |
denotes |
neurons |
T1809 |
2949-2952 |
DT |
denotes |
the |
T1810 |
2958-2963 |
NN |
denotes |
cycle |
T1811 |
2953-2957 |
NN |
denotes |
cell |
T1812 |
2963-2964 |
. |
denotes |
. |
T1813 |
2964-3134 |
sentence |
denotes |
An emerging principle from work in Drosophila and vertebrates is that target-derived factors play a crucial role in the induction of these transcriptional programs [10]. |
T1814 |
2965-2967 |
DT |
denotes |
An |
T1815 |
2977-2986 |
NN |
denotes |
principle |
T1816 |
2968-2976 |
VBG |
denotes |
emerging |
T1817 |
3027-3029 |
VBZ |
denotes |
is |
T1818 |
2987-2991 |
IN |
denotes |
from |
T1819 |
2992-2996 |
NN |
denotes |
work |
T1820 |
2997-2999 |
IN |
denotes |
in |
T1821 |
3000-3010 |
NNP |
denotes |
Drosophila |
T1822 |
3011-3014 |
CC |
denotes |
and |
T1823 |
3015-3026 |
NNS |
denotes |
vertebrates |
T1824 |
3030-3034 |
IN |
denotes |
that |
T1825 |
3058-3062 |
VBP |
denotes |
play |
T1826 |
3035-3041 |
NN |
denotes |
target |
T1827 |
3042-3049 |
VBN |
denotes |
derived |
T1828 |
3041-3042 |
HYPH |
denotes |
- |
T1829 |
3050-3057 |
NNS |
denotes |
factors |
T1830 |
3063-3064 |
DT |
denotes |
a |
T1831 |
3073-3077 |
NN |
denotes |
role |
T1832 |
3065-3072 |
JJ |
denotes |
crucial |
T1833 |
3078-3080 |
IN |
denotes |
in |
T1834 |
3081-3084 |
DT |
denotes |
the |
T1835 |
3085-3094 |
NN |
denotes |
induction |
T1836 |
3095-3097 |
IN |
denotes |
of |
T1837 |
3098-3103 |
DT |
denotes |
these |
T1838 |
3120-3128 |
NNS |
denotes |
programs |
T1839 |
3104-3119 |
JJ |
denotes |
transcriptional |
T1840 |
3129-3130 |
-LRB- |
denotes |
[ |
T1841 |
3130-3132 |
CD |
denotes |
10 |
T1842 |
3132-3133 |
-RRB- |
denotes |
] |
T1843 |
3133-3134 |
. |
denotes |
. |
T1844 |
3134-3315 |
sentence |
denotes |
In Drosophila, retrograde BMP signals from the target region control the terminal differentiation of a subpopulation of peptidergic neurons expressing Apterous and Squeeze [11,12]. |
T1845 |
3135-3137 |
IN |
denotes |
In |
T1846 |
3196-3203 |
VBP |
denotes |
control |
T1847 |
3138-3148 |
NNP |
denotes |
Drosophila |
T1848 |
3148-3150 |
, |
denotes |
, |
T1849 |
3150-3160 |
JJ |
denotes |
retrograde |
T1850 |
3165-3172 |
NNS |
denotes |
signals |
T1851 |
3161-3164 |
NN |
denotes |
BMP |
T1852 |
3173-3177 |
IN |
denotes |
from |
T1853 |
3178-3181 |
DT |
denotes |
the |
T1854 |
3189-3195 |
NN |
denotes |
region |
T1855 |
3182-3188 |
NN |
denotes |
target |
T1856 |
3204-3207 |
DT |
denotes |
the |
T1857 |
3217-3232 |
NN |
denotes |
differentiation |
T1858 |
3208-3216 |
JJ |
denotes |
terminal |
T1859 |
3233-3235 |
IN |
denotes |
of |
T1860 |
3236-3237 |
DT |
denotes |
a |
T1861 |
3238-3251 |
NN |
denotes |
subpopulation |
T1862 |
3252-3254 |
IN |
denotes |
of |
T1863 |
3255-3266 |
JJ |
denotes |
peptidergic |
T1864 |
3267-3274 |
NNS |
denotes |
neurons |
T1865 |
3275-3285 |
VBG |
denotes |
expressing |
T1866 |
3286-3294 |
NN |
denotes |
Apterous |
T1867 |
3295-3298 |
CC |
denotes |
and |
T1868 |
3299-3306 |
NN |
denotes |
Squeeze |
T1869 |
3307-3308 |
-LRB- |
denotes |
[ |
T1870 |
3311-3313 |
CD |
denotes |
12 |
T1871 |
3308-3310 |
CD |
denotes |
11 |
T1872 |
3310-3311 |
, |
denotes |
, |
T1873 |
3313-3314 |
-RRB- |
denotes |
] |
T1874 |
3314-3315 |
. |
denotes |
. |
T1875 |
3315-3581 |
sentence |
denotes |
In vertebrates, peripheral neurotrophic signals have been shown to direct the onset of expression of the ETS transcription factors Er81 and Pea3 in DRG sensory neurons and motor neuron pools several days after these neurons have become post-mitotic [9,13,14,15,16]. |
T1876 |
3316-3318 |
IN |
denotes |
In |
T1877 |
3374-3379 |
VBN |
denotes |
shown |
T1878 |
3319-3330 |
NNS |
denotes |
vertebrates |
T1879 |
3330-3332 |
, |
denotes |
, |
T1880 |
3332-3342 |
JJ |
denotes |
peripheral |
T1881 |
3356-3363 |
NNS |
denotes |
signals |
T1882 |
3343-3355 |
JJ |
denotes |
neurotrophic |
T1883 |
3364-3368 |
VBP |
denotes |
have |
T1884 |
3369-3373 |
VBN |
denotes |
been |
T1885 |
3380-3382 |
TO |
denotes |
to |
T1886 |
3383-3389 |
VB |
denotes |
direct |
T1887 |
3390-3393 |
DT |
denotes |
the |
T1888 |
3394-3399 |
NN |
denotes |
onset |
T1889 |
3400-3402 |
IN |
denotes |
of |
T1890 |
3403-3413 |
NN |
denotes |
expression |
T1891 |
3414-3416 |
IN |
denotes |
of |
T1892 |
3417-3420 |
DT |
denotes |
the |
T1893 |
3439-3446 |
NNS |
denotes |
factors |
T1894 |
3421-3424 |
NN |
denotes |
ETS |
T1895 |
3425-3438 |
NN |
denotes |
transcription |
T1896 |
3447-3451 |
NN |
denotes |
Er81 |
T1897 |
3452-3455 |
CC |
denotes |
and |
T1898 |
3456-3460 |
NN |
denotes |
Pea3 |
T1899 |
3461-3463 |
IN |
denotes |
in |
T1900 |
3464-3467 |
NN |
denotes |
DRG |
T1901 |
3476-3483 |
NNS |
denotes |
neurons |
T1902 |
3468-3475 |
JJ |
denotes |
sensory |
T1903 |
3484-3487 |
CC |
denotes |
and |
T1904 |
3488-3493 |
NN |
denotes |
motor |
T1905 |
3501-3506 |
NNS |
denotes |
pools |
T1906 |
3494-3500 |
NN |
denotes |
neuron |
T1907 |
3507-3514 |
JJ |
denotes |
several |
T1908 |
3515-3519 |
NNS |
denotes |
days |
T1909 |
3545-3551 |
VBN |
denotes |
become |
T1910 |
3520-3525 |
IN |
denotes |
after |
T1911 |
3526-3531 |
DT |
denotes |
these |
T1912 |
3532-3539 |
NNS |
denotes |
neurons |
T1913 |
3540-3544 |
VBP |
denotes |
have |
T1914 |
3552-3564 |
JJ |
denotes |
post-mitotic |
T1915 |
3565-3566 |
-LRB- |
denotes |
[ |
T1916 |
3577-3579 |
CD |
denotes |
16 |
T1917 |
3566-3567 |
CD |
denotes |
9 |
T1918 |
3567-3568 |
, |
denotes |
, |
T1919 |
3568-3570 |
CD |
denotes |
13 |
T1920 |
3570-3571 |
, |
denotes |
, |
T1921 |
3571-3573 |
CD |
denotes |
14 |
T1922 |
3573-3574 |
, |
denotes |
, |
T1923 |
3574-3576 |
CD |
denotes |
15 |
T1924 |
3576-3577 |
, |
denotes |
, |
T1925 |
3579-3580 |
-RRB- |
denotes |
] |
T1926 |
3580-3581 |
. |
denotes |
. |
T1927 |
3581-3717 |
sentence |
denotes |
Moreover, the induction of Er81 expression in proprioceptive afferents is known to be mediated by peripheral neurotrophin 3 (NT-3) [9]. |
T1928 |
3582-3590 |
RB |
denotes |
Moreover |
T1929 |
3656-3661 |
VBN |
denotes |
known |
T1930 |
3590-3592 |
, |
denotes |
, |
T1931 |
3592-3595 |
DT |
denotes |
the |
T1932 |
3596-3605 |
NN |
denotes |
induction |
T1933 |
3606-3608 |
IN |
denotes |
of |
T1934 |
3609-3613 |
NN |
denotes |
Er81 |
T1935 |
3614-3624 |
NN |
denotes |
expression |
T1936 |
3625-3627 |
IN |
denotes |
in |
T1937 |
3628-3642 |
JJ |
denotes |
proprioceptive |
T1938 |
3643-3652 |
NNS |
denotes |
afferents |
T1939 |
3653-3655 |
VBZ |
denotes |
is |
T1940 |
3662-3664 |
TO |
denotes |
to |
T1941 |
3668-3676 |
VBN |
denotes |
mediated |
T1942 |
3665-3667 |
VB |
denotes |
be |
T1943 |
3677-3679 |
IN |
denotes |
by |
T1944 |
3680-3690 |
JJ |
denotes |
peripheral |
T1945 |
3691-3703 |
NN |
denotes |
neurotrophin |
T1946 |
3704-3705 |
CD |
denotes |
3 |
T1947 |
3706-3707 |
-LRB- |
denotes |
( |
T1948 |
3707-3709 |
NN |
denotes |
NT |
T1949 |
3709-3710 |
HYPH |
denotes |
- |
T1950 |
3710-3711 |
CD |
denotes |
3 |
T1951 |
3711-3712 |
-RRB- |
denotes |
) |
T1952 |
3713-3714 |
-LRB- |
denotes |
[ |
T1953 |
3714-3715 |
CD |
denotes |
9 |
T1954 |
3715-3716 |
-RRB- |
denotes |
] |
T1955 |
3716-3717 |
. |
denotes |
. |
T1956 |
3717-3944 |
sentence |
denotes |
These two ETS proteins control late aspects of spinal monosynaptic circuit assembly, with Er81 directing proprioceptive sensory neuron differentiation and Pea3 directing motor neuron pool differentiation, respectively [14,15]. |
T1957 |
3718-3723 |
DT |
denotes |
These |
T1958 |
3732-3740 |
NN |
denotes |
proteins |
T1959 |
3724-3727 |
CD |
denotes |
two |
T1960 |
3728-3731 |
NN |
denotes |
ETS |
T1961 |
3741-3748 |
VBP |
denotes |
control |
T1962 |
3749-3753 |
JJ |
denotes |
late |
T1963 |
3754-3761 |
NNS |
denotes |
aspects |
T1964 |
3762-3764 |
IN |
denotes |
of |
T1965 |
3765-3771 |
JJ |
denotes |
spinal |
T1966 |
3785-3792 |
NN |
denotes |
circuit |
T1967 |
3772-3784 |
JJ |
denotes |
monosynaptic |
T1968 |
3793-3801 |
NN |
denotes |
assembly |
T1969 |
3801-3803 |
, |
denotes |
, |
T1970 |
3803-3807 |
IN |
denotes |
with |
T1971 |
3808-3812 |
NN |
denotes |
Er81 |
T1972 |
3813-3822 |
VBG |
denotes |
directing |
T1973 |
3823-3837 |
JJ |
denotes |
proprioceptive |
T1974 |
3846-3852 |
NN |
denotes |
neuron |
T1975 |
3838-3845 |
JJ |
denotes |
sensory |
T1976 |
3853-3868 |
NN |
denotes |
differentiation |
T1977 |
3869-3872 |
CC |
denotes |
and |
T1978 |
3873-3877 |
NN |
denotes |
Pea3 |
T1979 |
3878-3887 |
VBG |
denotes |
directing |
T1980 |
3888-3893 |
NN |
denotes |
motor |
T1981 |
3901-3905 |
NN |
denotes |
pool |
T1982 |
3894-3900 |
NN |
denotes |
neuron |
T1983 |
3906-3921 |
NN |
denotes |
differentiation |
T1984 |
3921-3923 |
, |
denotes |
, |
T1985 |
3923-3935 |
RB |
denotes |
respectively |
T1986 |
3936-3937 |
-LRB- |
denotes |
[ |
T1987 |
3940-3942 |
CD |
denotes |
15 |
T1988 |
3937-3939 |
CD |
denotes |
14 |
T1989 |
3939-3940 |
, |
denotes |
, |
T1990 |
3942-3943 |
-RRB- |
denotes |
] |
T1991 |
3943-3944 |
. |
denotes |
. |
T1992 |
3944-4215 |
sentence |
denotes |
In particular, in the absence of Er81, achieved by mutation in the gene or by deprivation of peripheral neurotrophin signaling, group Ia proprioceptive afferents fail to invade the ventral spinal cord and to make effective synaptic connections with motor neurons [9,14]. |
T1993 |
3945-3947 |
IN |
denotes |
In |
T1994 |
4107-4111 |
VBP |
denotes |
fail |
T1995 |
3948-3958 |
JJ |
denotes |
particular |
T1996 |
3958-3960 |
, |
denotes |
, |
T1997 |
3960-3962 |
IN |
denotes |
in |
T1998 |
3963-3966 |
DT |
denotes |
the |
T1999 |
3967-3974 |
NN |
denotes |
absence |
T2000 |
3975-3977 |
IN |
denotes |
of |
T2001 |
3978-3982 |
NN |
denotes |
Er81 |
T2002 |
3982-3984 |
, |
denotes |
, |
T2003 |
3984-3992 |
VBN |
denotes |
achieved |
T2004 |
3993-3995 |
IN |
denotes |
by |
T2005 |
3996-4004 |
NN |
denotes |
mutation |
T2006 |
4005-4007 |
IN |
denotes |
in |
T2007 |
4008-4011 |
DT |
denotes |
the |
T2008 |
4012-4016 |
NN |
denotes |
gene |
T2009 |
4017-4019 |
CC |
denotes |
or |
T2010 |
4020-4022 |
IN |
denotes |
by |
T2011 |
4023-4034 |
NN |
denotes |
deprivation |
T2012 |
4035-4037 |
IN |
denotes |
of |
T2013 |
4038-4048 |
JJ |
denotes |
peripheral |
T2014 |
4062-4071 |
NN |
denotes |
signaling |
T2015 |
4049-4061 |
NN |
denotes |
neurotrophin |
T2016 |
4071-4073 |
, |
denotes |
, |
T2017 |
4073-4078 |
NN |
denotes |
group |
T2018 |
4079-4081 |
NN |
denotes |
Ia |
T2019 |
4097-4106 |
NNS |
denotes |
afferents |
T2020 |
4082-4096 |
JJ |
denotes |
proprioceptive |
T2021 |
4112-4114 |
TO |
denotes |
to |
T2022 |
4115-4121 |
VB |
denotes |
invade |
T2023 |
4122-4125 |
DT |
denotes |
the |
T2024 |
4141-4145 |
NN |
denotes |
cord |
T2025 |
4126-4133 |
JJ |
denotes |
ventral |
T2026 |
4134-4140 |
JJ |
denotes |
spinal |
T2027 |
4146-4149 |
CC |
denotes |
and |
T2028 |
4150-4152 |
TO |
denotes |
to |
T2029 |
4153-4157 |
VB |
denotes |
make |
T2030 |
4158-4167 |
JJ |
denotes |
effective |
T2031 |
4177-4188 |
NNS |
denotes |
connections |
T2032 |
4168-4176 |
JJ |
denotes |
synaptic |
T2033 |
4189-4193 |
IN |
denotes |
with |
T2034 |
4194-4199 |
NN |
denotes |
motor |
T2035 |
4200-4207 |
NNS |
denotes |
neurons |
T2036 |
4208-4209 |
-LRB- |
denotes |
[ |
T2037 |
4211-4213 |
CD |
denotes |
14 |
T2038 |
4209-4210 |
CD |
denotes |
9 |
T2039 |
4210-4211 |
, |
denotes |
, |
T2040 |
4213-4214 |
-RRB- |
denotes |
] |
T2041 |
4214-4215 |
. |
denotes |
. |
T2042 |
4215-4424 |
sentence |
denotes |
The involvement of target-derived signals in induction of ETS transcription factor expression raises the question of the necessity for the observed delay in the onset of ETS signaling for neuronal maturation. |
T2043 |
4216-4219 |
DT |
denotes |
The |
T2044 |
4220-4231 |
NN |
denotes |
involvement |
T2045 |
4310-4316 |
VBZ |
denotes |
raises |
T2046 |
4232-4234 |
IN |
denotes |
of |
T2047 |
4235-4241 |
NN |
denotes |
target |
T2048 |
4242-4249 |
VBN |
denotes |
derived |
T2049 |
4241-4242 |
HYPH |
denotes |
- |
T2050 |
4250-4257 |
NNS |
denotes |
signals |
T2051 |
4258-4260 |
IN |
denotes |
in |
T2052 |
4261-4270 |
NN |
denotes |
induction |
T2053 |
4271-4273 |
IN |
denotes |
of |
T2054 |
4274-4277 |
NN |
denotes |
ETS |
T2055 |
4299-4309 |
NN |
denotes |
expression |
T2056 |
4278-4291 |
NN |
denotes |
transcription |
T2057 |
4292-4298 |
NN |
denotes |
factor |
T2058 |
4317-4320 |
DT |
denotes |
the |
T2059 |
4321-4329 |
NN |
denotes |
question |
T2060 |
4330-4332 |
IN |
denotes |
of |
T2061 |
4333-4336 |
DT |
denotes |
the |
T2062 |
4337-4346 |
NN |
denotes |
necessity |
T2063 |
4347-4350 |
IN |
denotes |
for |
T2064 |
4351-4354 |
DT |
denotes |
the |
T2065 |
4364-4369 |
NN |
denotes |
delay |
T2066 |
4355-4363 |
VBN |
denotes |
observed |
T2067 |
4370-4372 |
IN |
denotes |
in |
T2068 |
4373-4376 |
DT |
denotes |
the |
T2069 |
4377-4382 |
NN |
denotes |
onset |
T2070 |
4383-4385 |
IN |
denotes |
of |
T2071 |
4386-4389 |
NN |
denotes |
ETS |
T2072 |
4390-4399 |
NN |
denotes |
signaling |
T2073 |
4400-4403 |
IN |
denotes |
for |
T2074 |
4404-4412 |
JJ |
denotes |
neuronal |
T2075 |
4413-4423 |
NN |
denotes |
maturation |
T2076 |
4423-4424 |
. |
denotes |
. |
T2077 |
4424-4559 |
sentence |
denotes |
Would precocious expression of ETS proteins in post-mitotic neurons also direct the appropriate sensory neuron developmental programs? |
T2078 |
4425-4430 |
MD |
denotes |
Would |
T2079 |
4498-4504 |
VB |
denotes |
direct |
T2080 |
4431-4441 |
JJ |
denotes |
precocious |
T2081 |
4442-4452 |
NN |
denotes |
expression |
T2082 |
4453-4455 |
IN |
denotes |
of |
T2083 |
4456-4459 |
NN |
denotes |
ETS |
T2084 |
4460-4468 |
NN |
denotes |
proteins |
T2085 |
4469-4471 |
IN |
denotes |
in |
T2086 |
4472-4484 |
JJ |
denotes |
post-mitotic |
T2087 |
4485-4492 |
NNS |
denotes |
neurons |
T2088 |
4493-4497 |
RB |
denotes |
also |
T2089 |
4505-4508 |
DT |
denotes |
the |
T2090 |
4550-4558 |
NNS |
denotes |
programs |
T2091 |
4509-4520 |
JJ |
denotes |
appropriate |
T2092 |
4521-4528 |
JJ |
denotes |
sensory |
T2093 |
4529-4535 |
NN |
denotes |
neuron |
T2094 |
4536-4549 |
JJ |
denotes |
developmental |
T2095 |
4558-4559 |
. |
denotes |
? |
T2096 |
4559-4772 |
sentence |
denotes |
In this study, we have used mouse genetics to test this general idea, by investigating whether the precise timing of onset of ETS transcription factor signaling is essential for normal sensory neuron development. |
T2097 |
4560-4562 |
IN |
denotes |
In |
T2098 |
4583-4587 |
VBN |
denotes |
used |
T2099 |
4563-4567 |
DT |
denotes |
this |
T2100 |
4568-4573 |
NN |
denotes |
study |
T2101 |
4573-4575 |
, |
denotes |
, |
T2102 |
4575-4577 |
PRP |
denotes |
we |
T2103 |
4578-4582 |
VBP |
denotes |
have |
T2104 |
4588-4593 |
NN |
denotes |
mouse |
T2105 |
4594-4602 |
NN |
denotes |
genetics |
T2106 |
4603-4605 |
TO |
denotes |
to |
T2107 |
4606-4610 |
VB |
denotes |
test |
T2108 |
4611-4615 |
DT |
denotes |
this |
T2109 |
4624-4628 |
NN |
denotes |
idea |
T2110 |
4616-4623 |
JJ |
denotes |
general |
T2111 |
4628-4630 |
, |
denotes |
, |
T2112 |
4630-4632 |
IN |
denotes |
by |
T2113 |
4633-4646 |
VBG |
denotes |
investigating |
T2114 |
4647-4654 |
IN |
denotes |
whether |
T2115 |
4721-4723 |
VBZ |
denotes |
is |
T2116 |
4655-4658 |
DT |
denotes |
the |
T2117 |
4667-4673 |
NN |
denotes |
timing |
T2118 |
4659-4666 |
JJ |
denotes |
precise |
T2119 |
4674-4676 |
IN |
denotes |
of |
T2120 |
4677-4682 |
NN |
denotes |
onset |
T2121 |
4683-4685 |
IN |
denotes |
of |
T2122 |
4686-4689 |
NN |
denotes |
ETS |
T2123 |
4711-4720 |
NN |
denotes |
signaling |
T2124 |
4690-4703 |
NN |
denotes |
transcription |
T2125 |
4704-4710 |
NN |
denotes |
factor |
T2126 |
4724-4733 |
JJ |
denotes |
essential |
T2127 |
4734-4737 |
IN |
denotes |
for |
T2128 |
4738-4744 |
JJ |
denotes |
normal |
T2129 |
4760-4771 |
NN |
denotes |
development |
T2130 |
4745-4752 |
JJ |
denotes |
sensory |
T2131 |
4753-4759 |
NN |
denotes |
neuron |
T2132 |
4771-4772 |
. |
denotes |
. |
T2133 |
4772-4897 |
sentence |
denotes |
We have assessed the biological effects of inducing ETS signaling either at the correct developmental time, or precociously. |
T2134 |
4773-4775 |
PRP |
denotes |
We |
T2135 |
4781-4789 |
VBN |
denotes |
assessed |
T2136 |
4776-4780 |
VBP |
denotes |
have |
T2137 |
4790-4793 |
DT |
denotes |
the |
T2138 |
4805-4812 |
NNS |
denotes |
effects |
T2139 |
4794-4804 |
JJ |
denotes |
biological |
T2140 |
4813-4815 |
IN |
denotes |
of |
T2141 |
4816-4824 |
VBG |
denotes |
inducing |
T2142 |
4825-4828 |
NN |
denotes |
ETS |
T2143 |
4829-4838 |
NN |
denotes |
signaling |
T2144 |
4839-4845 |
CC |
denotes |
either |
T2145 |
4846-4848 |
IN |
denotes |
at |
T2146 |
4849-4852 |
DT |
denotes |
the |
T2147 |
4875-4879 |
NN |
denotes |
time |
T2148 |
4853-4860 |
JJ |
denotes |
correct |
T2149 |
4861-4874 |
JJ |
denotes |
developmental |
T2150 |
4879-4881 |
, |
denotes |
, |
T2151 |
4881-4883 |
CC |
denotes |
or |
T2152 |
4884-4896 |
RB |
denotes |
precociously |
T2153 |
4896-4897 |
. |
denotes |
. |
T2154 |
4897-5075 |
sentence |
denotes |
We find that within proprioceptive sensory neurons, the late onset of ETS signaling is essential for the establishment of normal sensory afferent projections in the spinal cord. |
T2155 |
4898-4900 |
PRP |
denotes |
We |
T2156 |
4901-4905 |
VBP |
denotes |
find |
T2157 |
4906-4910 |
IN |
denotes |
that |
T2158 |
4982-4984 |
VBZ |
denotes |
is |
T2159 |
4911-4917 |
IN |
denotes |
within |
T2160 |
4918-4932 |
JJ |
denotes |
proprioceptive |
T2161 |
4941-4948 |
NNS |
denotes |
neurons |
T2162 |
4933-4940 |
JJ |
denotes |
sensory |
T2163 |
4948-4950 |
, |
denotes |
, |
T2164 |
4950-4953 |
DT |
denotes |
the |
T2165 |
4959-4964 |
NN |
denotes |
onset |
T2166 |
4954-4958 |
JJ |
denotes |
late |
T2167 |
4965-4967 |
IN |
denotes |
of |
T2168 |
4968-4971 |
NN |
denotes |
ETS |
T2169 |
4972-4981 |
NN |
denotes |
signaling |
T2170 |
4985-4994 |
JJ |
denotes |
essential |
T2171 |
4995-4998 |
IN |
denotes |
for |
T2172 |
4999-5002 |
DT |
denotes |
the |
T2173 |
5003-5016 |
NN |
denotes |
establishment |
T2174 |
5017-5019 |
IN |
denotes |
of |
T2175 |
5020-5026 |
JJ |
denotes |
normal |
T2176 |
5044-5055 |
NNS |
denotes |
projections |
T2177 |
5027-5034 |
JJ |
denotes |
sensory |
T2178 |
5035-5043 |
JJ |
denotes |
afferent |
T2179 |
5056-5058 |
IN |
denotes |
in |
T2180 |
5059-5062 |
DT |
denotes |
the |
T2181 |
5070-5074 |
NN |
denotes |
cord |
T2182 |
5063-5069 |
JJ |
denotes |
spinal |
T2183 |
5074-5075 |
. |
denotes |
. |
T2184 |
5075-5294 |
sentence |
denotes |
Precocious initiation of ETS signaling in post-mitotic DRG neurons leads to abnormal DRG neuron differentiation characterized by neurotrophin-independent neurite outgrowth and inappropriate profiles of gene expression. |
T2185 |
5076-5086 |
JJ |
denotes |
Precocious |
T2186 |
5087-5097 |
NN |
denotes |
initiation |
T2187 |
5143-5148 |
VBZ |
denotes |
leads |
T2188 |
5098-5100 |
IN |
denotes |
of |
T2189 |
5101-5104 |
NN |
denotes |
ETS |
T2190 |
5105-5114 |
NN |
denotes |
signaling |
T2191 |
5115-5117 |
IN |
denotes |
in |
T2192 |
5118-5130 |
JJ |
denotes |
post-mitotic |
T2193 |
5135-5142 |
NNS |
denotes |
neurons |
T2194 |
5131-5134 |
NN |
denotes |
DRG |
T2195 |
5149-5151 |
IN |
denotes |
to |
T2196 |
5152-5160 |
JJ |
denotes |
abnormal |
T2197 |
5172-5187 |
NN |
denotes |
differentiation |
T2198 |
5161-5164 |
NN |
denotes |
DRG |
T2199 |
5165-5171 |
NN |
denotes |
neuron |
T2200 |
5188-5201 |
VBN |
denotes |
characterized |
T2201 |
5202-5204 |
IN |
denotes |
by |
T2202 |
5205-5217 |
NN |
denotes |
neurotrophin |
T2203 |
5218-5229 |
JJ |
denotes |
independent |
T2204 |
5217-5218 |
HYPH |
denotes |
- |
T2205 |
5238-5247 |
NN |
denotes |
outgrowth |
T2206 |
5230-5237 |
NN |
denotes |
neurite |
T2207 |
5248-5251 |
CC |
denotes |
and |
T2208 |
5252-5265 |
JJ |
denotes |
inappropriate |
T2209 |
5266-5274 |
NNS |
denotes |
profiles |
T2210 |
5275-5277 |
IN |
denotes |
of |
T2211 |
5278-5282 |
NN |
denotes |
gene |
T2212 |
5283-5293 |
NN |
denotes |
expression |
T2213 |
5293-5294 |
. |
denotes |
. |
T2214 |
5294-5564 |
sentence |
denotes |
Our findings reveal that target-triggered inductive signals provide an effective means of ensuring the late onset of expression of transcription factors, and thus an orderly temporal transcriptional sequence that is crucial for neuronal maturation and circuit assembly. |
T2215 |
5295-5298 |
PRP$ |
denotes |
Our |
T2216 |
5299-5307 |
NNS |
denotes |
findings |
T2217 |
5308-5314 |
VBP |
denotes |
reveal |
T2218 |
5315-5319 |
IN |
denotes |
that |
T2219 |
5355-5362 |
VBP |
denotes |
provide |
T2220 |
5320-5326 |
NN |
denotes |
target |
T2221 |
5327-5336 |
VBN |
denotes |
triggered |
T2222 |
5326-5327 |
HYPH |
denotes |
- |
T2223 |
5347-5354 |
NNS |
denotes |
signals |
T2224 |
5337-5346 |
JJ |
denotes |
inductive |
T2225 |
5363-5365 |
DT |
denotes |
an |
T2226 |
5376-5381 |
NNS |
denotes |
means |
T2227 |
5366-5375 |
JJ |
denotes |
effective |
T2228 |
5382-5384 |
IN |
denotes |
of |
T2229 |
5385-5393 |
VBG |
denotes |
ensuring |
T2230 |
5394-5397 |
DT |
denotes |
the |
T2231 |
5403-5408 |
NN |
denotes |
onset |
T2232 |
5398-5402 |
JJ |
denotes |
late |
T2233 |
5409-5411 |
IN |
denotes |
of |
T2234 |
5412-5422 |
NN |
denotes |
expression |
T2235 |
5423-5425 |
IN |
denotes |
of |
T2236 |
5426-5439 |
NN |
denotes |
transcription |
T2237 |
5440-5447 |
NNS |
denotes |
factors |
T2238 |
5447-5449 |
, |
denotes |
, |
T2239 |
5449-5452 |
CC |
denotes |
and |
T2240 |
5453-5457 |
RB |
denotes |
thus |
T2241 |
5494-5502 |
NN |
denotes |
sequence |
T2242 |
5458-5460 |
DT |
denotes |
an |
T2243 |
5461-5468 |
JJ |
denotes |
orderly |
T2244 |
5469-5477 |
JJ |
denotes |
temporal |
T2245 |
5478-5493 |
JJ |
denotes |
transcriptional |
T2246 |
5503-5507 |
WDT |
denotes |
that |
T2247 |
5508-5510 |
VBZ |
denotes |
is |
T2248 |
5511-5518 |
JJ |
denotes |
crucial |
T2249 |
5519-5522 |
IN |
denotes |
for |
T2250 |
5523-5531 |
JJ |
denotes |
neuronal |
T2251 |
5532-5542 |
NN |
denotes |
maturation |
T2252 |
5543-5546 |
CC |
denotes |
and |
T2253 |
5547-5554 |
NN |
denotes |
circuit |
T2254 |
5555-5563 |
NN |
denotes |
assembly |
T2255 |
5563-5564 |
. |
denotes |
. |
T2468 |
5574-5576 |
TO |
denotes |
To |
T2469 |
5577-5581 |
VB |
denotes |
test |
T2470 |
5726-5733 |
VBD |
denotes |
studied |
T2471 |
5582-5585 |
DT |
denotes |
the |
T2472 |
5586-5596 |
NN |
denotes |
hypothesis |
T2473 |
5597-5601 |
IN |
denotes |
that |
T2474 |
5660-5662 |
VBZ |
denotes |
is |
T2475 |
5602-5603 |
DT |
denotes |
a |
T2476 |
5613-5618 |
NN |
denotes |
delay |
T2477 |
5604-5612 |
JJ |
denotes |
temporal |
T2478 |
5619-5621 |
IN |
denotes |
in |
T2479 |
5622-5625 |
DT |
denotes |
the |
T2480 |
5626-5631 |
NN |
denotes |
onset |
T2481 |
5632-5634 |
IN |
denotes |
of |
T2482 |
5635-5650 |
JJ |
denotes |
transcriptional |
T2483 |
5651-5659 |
NNS |
denotes |
programs |
T2484 |
5663-5670 |
JJ |
denotes |
crucial |
T2485 |
5671-5674 |
IN |
denotes |
for |
T2486 |
5675-5678 |
DT |
denotes |
the |
T2487 |
5679-5686 |
NN |
denotes |
control |
T2488 |
5687-5689 |
IN |
denotes |
of |
T2489 |
5690-5701 |
JJ |
denotes |
appropriate |
T2490 |
5711-5721 |
NN |
denotes |
maturation |
T2491 |
5702-5710 |
JJ |
denotes |
neuronal |
T2492 |
5721-5723 |
, |
denotes |
, |
T2493 |
5723-5725 |
PRP |
denotes |
we |
T2494 |
5734-5737 |
DT |
denotes |
the |
T2495 |
5738-5749 |
NN |
denotes |
development |
T2496 |
5750-5752 |
IN |
denotes |
of |
T2497 |
5753-5767 |
JJ |
denotes |
proprioceptive |
T2498 |
5772-5779 |
NNS |
denotes |
neurons |
T2499 |
5768-5771 |
NN |
denotes |
DRG |
T2500 |
5779-5781 |
, |
denotes |
, |
T2501 |
5781-5786 |
IN |
denotes |
since |
T2502 |
5916-5926 |
VBN |
denotes |
identified |
T2503 |
5787-5802 |
JJ |
denotes |
transcriptional |
T2504 |
5803-5812 |
NNS |
denotes |
effectors |
T2505 |
5813-5822 |
VBN |
denotes |
regulated |
T2506 |
5823-5825 |
IN |
denotes |
by |
T2507 |
5826-5832 |
NN |
denotes |
target |
T2508 |
5833-5840 |
VBN |
denotes |
derived |
T2509 |
5832-5833 |
HYPH |
denotes |
- |
T2510 |
5841-5848 |
NNS |
denotes |
signals |
T2511 |
5848-5850 |
, |
denotes |
, |
T2512 |
5850-5852 |
RB |
denotes |
as |
T2513 |
5858-5860 |
IN |
denotes |
as |
T2514 |
5853-5857 |
RB |
denotes |
well |
T2515 |
5861-5865 |
DT |
denotes |
some |
T2516 |
5866-5868 |
IN |
denotes |
of |
T2517 |
5869-5874 |
PRP$ |
denotes |
their |
T2518 |
5897-5904 |
NNS |
denotes |
actions |
T2519 |
5875-5885 |
JJ |
denotes |
downstream |
T2520 |
5886-5896 |
JJ |
denotes |
biological |
T2521 |
5904-5906 |
, |
denotes |
, |
T2522 |
5906-5910 |
VBP |
denotes |
have |
T2523 |
5911-5915 |
VBN |
denotes |
been |
T2524 |
5927-5930 |
IN |
denotes |
for |
T2525 |
5931-5936 |
DT |
denotes |
these |
T2526 |
5937-5944 |
NNS |
denotes |
neurons |
T2527 |
5944-5945 |
. |
denotes |
. |
T2528 |
5945-6222 |
sentence |
denotes |
Er81 controls proprioceptive afferent connectivity [14], and we therefore sought to identify an ETS transcriptional regulator that, when expressed over the normal time course of Er81 expression, is able to substitute for Er81 function within group Ia afferent sensory neurons. |
T2529 |
5946-5950 |
NN |
denotes |
Er81 |
T2530 |
5951-5959 |
VBZ |
denotes |
controls |
T2531 |
5960-5974 |
JJ |
denotes |
proprioceptive |
T2532 |
5984-5996 |
NN |
denotes |
connectivity |
T2533 |
5975-5983 |
JJ |
denotes |
afferent |
T2534 |
5997-5998 |
-LRB- |
denotes |
[ |
T2535 |
5998-6000 |
CD |
denotes |
14 |
T2536 |
6000-6001 |
-RRB- |
denotes |
] |
T2537 |
6001-6003 |
, |
denotes |
, |
T2538 |
6003-6006 |
CC |
denotes |
and |
T2539 |
6007-6009 |
PRP |
denotes |
we |
T2540 |
6020-6026 |
VBD |
denotes |
sought |
T2541 |
6010-6019 |
RB |
denotes |
therefore |
T2542 |
6027-6029 |
TO |
denotes |
to |
T2543 |
6030-6038 |
VB |
denotes |
identify |
T2544 |
6039-6041 |
DT |
denotes |
an |
T2545 |
6062-6071 |
NN |
denotes |
regulator |
T2546 |
6042-6045 |
NN |
denotes |
ETS |
T2547 |
6046-6061 |
JJ |
denotes |
transcriptional |
T2548 |
6072-6076 |
WDT |
denotes |
that |
T2549 |
6141-6143 |
VBZ |
denotes |
is |
T2550 |
6076-6078 |
, |
denotes |
, |
T2551 |
6078-6082 |
WRB |
denotes |
when |
T2552 |
6083-6092 |
VBN |
denotes |
expressed |
T2553 |
6093-6097 |
IN |
denotes |
over |
T2554 |
6098-6101 |
DT |
denotes |
the |
T2555 |
6114-6120 |
NN |
denotes |
course |
T2556 |
6102-6108 |
JJ |
denotes |
normal |
T2557 |
6109-6113 |
NN |
denotes |
time |
T2558 |
6121-6123 |
IN |
denotes |
of |
T2559 |
6124-6128 |
NN |
denotes |
Er81 |
T2560 |
6129-6139 |
NN |
denotes |
expression |
T2561 |
6139-6141 |
, |
denotes |
, |
T2562 |
6144-6148 |
JJ |
denotes |
able |
T2563 |
6149-6151 |
TO |
denotes |
to |
T2564 |
6152-6162 |
VB |
denotes |
substitute |
T2565 |
6163-6166 |
IN |
denotes |
for |
T2566 |
6167-6171 |
NN |
denotes |
Er81 |
T2567 |
6172-6180 |
NN |
denotes |
function |
T2568 |
6181-6187 |
IN |
denotes |
within |
T2569 |
6188-6193 |
NN |
denotes |
group |
T2570 |
6194-6196 |
NN |
denotes |
Ia |
T2571 |
6214-6221 |
NNS |
denotes |
neurons |
T2572 |
6197-6205 |
JJ |
denotes |
afferent |
T2573 |
6206-6213 |
JJ |
denotes |
sensory |
T2574 |
6221-6222 |
. |
denotes |
. |
T2575 |
6222-6411 |
sentence |
denotes |
With this reference point, we then designed experiments to examine the effects of precocious post-mitotic expression of the same ETS transcription factor on sensory neuron differentiation. |
T2576 |
6223-6227 |
IN |
denotes |
With |
T2577 |
6258-6266 |
VBD |
denotes |
designed |
T2578 |
6228-6232 |
DT |
denotes |
this |
T2579 |
6243-6248 |
NN |
denotes |
point |
T2580 |
6233-6242 |
NN |
denotes |
reference |
T2581 |
6248-6250 |
, |
denotes |
, |
T2582 |
6250-6252 |
PRP |
denotes |
we |
T2583 |
6253-6257 |
RB |
denotes |
then |
T2584 |
6267-6278 |
NNS |
denotes |
experiments |
T2585 |
6279-6281 |
TO |
denotes |
to |
T2586 |
6282-6289 |
VB |
denotes |
examine |
T2587 |
6290-6293 |
DT |
denotes |
the |
T2588 |
6294-6301 |
NNS |
denotes |
effects |
T2589 |
6302-6304 |
IN |
denotes |
of |
T2590 |
6305-6315 |
JJ |
denotes |
precocious |
T2591 |
6329-6339 |
NN |
denotes |
expression |
T2592 |
6316-6328 |
JJ |
denotes |
post-mitotic |
T2593 |
6340-6342 |
IN |
denotes |
of |
T2594 |
6343-6346 |
DT |
denotes |
the |
T2595 |
6370-6376 |
NN |
denotes |
factor |
T2596 |
6347-6351 |
JJ |
denotes |
same |
T2597 |
6352-6355 |
NN |
denotes |
ETS |
T2598 |
6356-6369 |
NN |
denotes |
transcription |
T2599 |
6377-6379 |
IN |
denotes |
on |
T2600 |
6380-6387 |
JJ |
denotes |
sensory |
T2601 |
6388-6394 |
NN |
denotes |
neuron |
T2602 |
6395-6410 |
NN |
denotes |
differentiation |
T2603 |
6410-6411 |
. |
denotes |
. |
T3609 |
6413-6416 |
NN |
denotes |
EWS |
T3610 |
6417-6421 |
NN |
denotes |
Pea3 |
T3611 |
6416-6417 |
HYPH |
denotes |
- |
T3612 |
6426-6433 |
VB |
denotes |
Replace |
T3613 |
6422-6425 |
MD |
denotes |
Can |
T3614 |
6434-6438 |
NN |
denotes |
Er81 |
T3615 |
6439-6447 |
NN |
denotes |
Function |
T3616 |
6448-6450 |
IN |
denotes |
in |
T3617 |
6451-6462 |
VBG |
denotes |
Controlling |
T3618 |
6463-6465 |
NN |
denotes |
Ia |
T3619 |
6475-6486 |
NNS |
denotes |
Projections |
T3620 |
6466-6474 |
JJ |
denotes |
Afferent |
T3621 |
6486-6663 |
sentence |
denotes |
We first defined an ETS transcription regulator that is able to replace the function of Er81 within proprioceptive afferents to direct projections into the ventral spinal cord. |
T3622 |
6487-6489 |
PRP |
denotes |
We |
T3623 |
6496-6503 |
VBD |
denotes |
defined |
T3624 |
6490-6495 |
RB |
denotes |
first |
T3625 |
6504-6506 |
DT |
denotes |
an |
T3626 |
6525-6534 |
NN |
denotes |
regulator |
T3627 |
6507-6510 |
NN |
denotes |
ETS |
T3628 |
6511-6524 |
NN |
denotes |
transcription |
T3629 |
6535-6539 |
WDT |
denotes |
that |
T3630 |
6540-6542 |
VBZ |
denotes |
is |
T3631 |
6543-6547 |
JJ |
denotes |
able |
T3632 |
6548-6550 |
TO |
denotes |
to |
T3633 |
6551-6558 |
VB |
denotes |
replace |
T3634 |
6559-6562 |
DT |
denotes |
the |
T3635 |
6563-6571 |
NN |
denotes |
function |
T3636 |
6572-6574 |
IN |
denotes |
of |
T3637 |
6575-6579 |
NN |
denotes |
Er81 |
T3638 |
6580-6586 |
IN |
denotes |
within |
T3639 |
6587-6601 |
JJ |
denotes |
proprioceptive |
T3640 |
6602-6611 |
NNS |
denotes |
afferents |
T3641 |
6612-6614 |
TO |
denotes |
to |
T3642 |
6615-6621 |
VB |
denotes |
direct |
T3643 |
6622-6633 |
NNS |
denotes |
projections |
T3644 |
6634-6638 |
IN |
denotes |
into |
T3645 |
6639-6642 |
DT |
denotes |
the |
T3646 |
6658-6662 |
NN |
denotes |
cord |
T3647 |
6643-6650 |
JJ |
denotes |
ventral |
T3648 |
6651-6657 |
JJ |
denotes |
spinal |
T3649 |
6662-6663 |
. |
denotes |
. |
T3650 |
6663-6844 |
sentence |
denotes |
Er81, Pea3, and Erm constitute the Pea3 subfamily of ETS transcription factors, show a high degree of amino acid identity, and bind to very similar DNA target sequences [17,18,19]. |
T3651 |
6664-6668 |
NN |
denotes |
Er81 |
T3652 |
6684-6694 |
VBP |
denotes |
constitute |
T3653 |
6668-6670 |
, |
denotes |
, |
T3654 |
6670-6674 |
NN |
denotes |
Pea3 |
T3655 |
6674-6676 |
, |
denotes |
, |
T3656 |
6676-6679 |
CC |
denotes |
and |
T3657 |
6680-6683 |
NN |
denotes |
Erm |
T3658 |
6695-6698 |
DT |
denotes |
the |
T3659 |
6704-6713 |
NN |
denotes |
subfamily |
T3660 |
6699-6703 |
NN |
denotes |
Pea3 |
T3661 |
6714-6716 |
IN |
denotes |
of |
T3662 |
6717-6720 |
NN |
denotes |
ETS |
T3663 |
6735-6742 |
NNS |
denotes |
factors |
T3664 |
6721-6734 |
NN |
denotes |
transcription |
T3665 |
6742-6744 |
, |
denotes |
, |
T3666 |
6744-6748 |
VBP |
denotes |
show |
T3667 |
6749-6750 |
DT |
denotes |
a |
T3668 |
6756-6762 |
NN |
denotes |
degree |
T3669 |
6751-6755 |
JJ |
denotes |
high |
T3670 |
6763-6765 |
IN |
denotes |
of |
T3671 |
6766-6771 |
NN |
denotes |
amino |
T3672 |
6772-6776 |
NN |
denotes |
acid |
T3673 |
6777-6785 |
NN |
denotes |
identity |
T3674 |
6785-6787 |
, |
denotes |
, |
T3675 |
6787-6790 |
CC |
denotes |
and |
T3676 |
6791-6795 |
VBP |
denotes |
bind |
T3677 |
6796-6798 |
IN |
denotes |
to |
T3678 |
6799-6803 |
RB |
denotes |
very |
T3679 |
6804-6811 |
JJ |
denotes |
similar |
T3680 |
6823-6832 |
NNS |
denotes |
sequences |
T3681 |
6812-6815 |
NN |
denotes |
DNA |
T3682 |
6816-6822 |
NN |
denotes |
target |
T3683 |
6833-6834 |
-LRB- |
denotes |
[ |
T3684 |
6840-6842 |
CD |
denotes |
19 |
T3685 |
6834-6836 |
CD |
denotes |
17 |
T3686 |
6836-6837 |
, |
denotes |
, |
T3687 |
6837-6839 |
CD |
denotes |
18 |
T3688 |
6839-6840 |
, |
denotes |
, |
T3689 |
6842-6843 |
-RRB- |
denotes |
] |
T3690 |
6843-6844 |
. |
denotes |
. |
T3691 |
6844-7118 |
sentence |
denotes |
Nevertheless, when introduced into the Er81 locus in analogy to a previously used targeting strategy (data not shown; [14]), neither Pea3 nor Erm could rescue Ia proprioceptive afferent projections to extensively invade the ventral horn of the spinal cord (data not shown). |
T3692 |
6845-6857 |
RB |
denotes |
Nevertheless |
T3693 |
6997-7003 |
VB |
denotes |
rescue |
T3694 |
6857-6859 |
, |
denotes |
, |
T3695 |
6859-6863 |
WRB |
denotes |
when |
T3696 |
6864-6874 |
VBN |
denotes |
introduced |
T3697 |
6875-6879 |
IN |
denotes |
into |
T3698 |
6880-6883 |
DT |
denotes |
the |
T3699 |
6889-6894 |
NN |
denotes |
locus |
T3700 |
6884-6888 |
NN |
denotes |
Er81 |
T3701 |
6895-6897 |
IN |
denotes |
in |
T3702 |
6898-6905 |
NN |
denotes |
analogy |
T3703 |
6906-6908 |
IN |
denotes |
to |
T3704 |
6909-6910 |
DT |
denotes |
a |
T3705 |
6937-6945 |
NN |
denotes |
strategy |
T3706 |
6911-6921 |
RB |
denotes |
previously |
T3707 |
6922-6926 |
VBN |
denotes |
used |
T3708 |
6927-6936 |
NN |
denotes |
targeting |
T3709 |
6946-6947 |
-LRB- |
denotes |
( |
T3710 |
6956-6961 |
VBN |
denotes |
shown |
T3711 |
6947-6951 |
NNS |
denotes |
data |
T3712 |
6952-6955 |
RB |
denotes |
not |
T3713 |
6961-6962 |
: |
denotes |
; |
T3714 |
6963-6964 |
-LRB- |
denotes |
[ |
T3715 |
6964-6966 |
CD |
denotes |
14 |
T3716 |
6966-6967 |
-RRB- |
denotes |
] |
T3717 |
6967-6968 |
-RRB- |
denotes |
) |
T3718 |
6968-6970 |
, |
denotes |
, |
T3719 |
6970-6977 |
CC |
denotes |
neither |
T3720 |
6978-6982 |
NN |
denotes |
Pea3 |
T3721 |
6983-6986 |
CC |
denotes |
nor |
T3722 |
6987-6990 |
NN |
denotes |
Erm |
T3723 |
6991-6996 |
MD |
denotes |
could |
T3724 |
7004-7006 |
NN |
denotes |
Ia |
T3725 |
7007-7021 |
JJ |
denotes |
proprioceptive |
T3726 |
7031-7042 |
NNS |
denotes |
projections |
T3727 |
7022-7030 |
JJ |
denotes |
afferent |
T3728 |
7043-7045 |
TO |
denotes |
to |
T3729 |
7058-7064 |
VB |
denotes |
invade |
T3730 |
7046-7057 |
RB |
denotes |
extensively |
T3731 |
7065-7068 |
DT |
denotes |
the |
T3732 |
7077-7081 |
NN |
denotes |
horn |
T3733 |
7069-7076 |
JJ |
denotes |
ventral |
T3734 |
7082-7084 |
IN |
denotes |
of |
T3735 |
7085-7088 |
DT |
denotes |
the |
T3736 |
7096-7100 |
NN |
denotes |
cord |
T3737 |
7089-7095 |
JJ |
denotes |
spinal |
T3738 |
7101-7102 |
-LRB- |
denotes |
( |
T3739 |
7111-7116 |
VBN |
denotes |
shown |
T3740 |
7102-7106 |
NNS |
denotes |
data |
T3741 |
7107-7110 |
RB |
denotes |
not |
T3742 |
7116-7117 |
-RRB- |
denotes |
) |
T3743 |
7117-7118 |
. |
denotes |
. |
T3744 |
7118-7362 |
sentence |
denotes |
These findings prompted us to analyze mice in which we integrated EWS-Pea3, a break-point fusion product between the amino-terminal domain of the Ewing sarcoma (EWS) gene and the Pea3 DNA binding domain [20,21], into the Er81 locus (Figure 1). |
T3745 |
7119-7124 |
DT |
denotes |
These |
T3746 |
7125-7133 |
NNS |
denotes |
findings |
T3747 |
7134-7142 |
VBD |
denotes |
prompted |
T3748 |
7143-7145 |
PRP |
denotes |
us |
T3749 |
7146-7148 |
TO |
denotes |
to |
T3750 |
7149-7156 |
VB |
denotes |
analyze |
T3751 |
7157-7161 |
NNS |
denotes |
mice |
T3752 |
7162-7164 |
IN |
denotes |
in |
T3753 |
7174-7184 |
VBD |
denotes |
integrated |
T3754 |
7165-7170 |
WDT |
denotes |
which |
T3755 |
7171-7173 |
PRP |
denotes |
we |
T3756 |
7185-7188 |
NN |
denotes |
EWS |
T3757 |
7189-7193 |
NN |
denotes |
Pea3 |
T3758 |
7188-7189 |
HYPH |
denotes |
- |
T3759 |
7193-7195 |
, |
denotes |
, |
T3760 |
7195-7196 |
DT |
denotes |
a |
T3761 |
7216-7223 |
NN |
denotes |
product |
T3762 |
7197-7202 |
NN |
denotes |
break |
T3763 |
7203-7208 |
NN |
denotes |
point |
T3764 |
7202-7203 |
HYPH |
denotes |
- |
T3765 |
7209-7215 |
NN |
denotes |
fusion |
T3766 |
7224-7231 |
IN |
denotes |
between |
T3767 |
7232-7235 |
DT |
denotes |
the |
T3768 |
7251-7257 |
NN |
denotes |
domain |
T3769 |
7236-7241 |
NN |
denotes |
amino |
T3770 |
7242-7250 |
JJ |
denotes |
terminal |
T3771 |
7241-7242 |
HYPH |
denotes |
- |
T3772 |
7258-7260 |
IN |
denotes |
of |
T3773 |
7261-7264 |
DT |
denotes |
the |
T3774 |
7285-7289 |
NN |
denotes |
gene |
T3775 |
7265-7270 |
NN |
denotes |
Ewing |
T3776 |
7271-7278 |
NN |
denotes |
sarcoma |
T3777 |
7279-7280 |
-LRB- |
denotes |
( |
T3778 |
7280-7283 |
NN |
denotes |
EWS |
T3779 |
7283-7284 |
-RRB- |
denotes |
) |
T3780 |
7290-7293 |
CC |
denotes |
and |
T3781 |
7294-7297 |
DT |
denotes |
the |
T3782 |
7315-7321 |
NN |
denotes |
domain |
T3783 |
7298-7302 |
NN |
denotes |
Pea3 |
T3784 |
7303-7306 |
NN |
denotes |
DNA |
T3785 |
7307-7314 |
VBG |
denotes |
binding |
T3786 |
7322-7323 |
-LRB- |
denotes |
[ |
T3787 |
7326-7328 |
CD |
denotes |
21 |
T3788 |
7323-7325 |
CD |
denotes |
20 |
T3789 |
7325-7326 |
, |
denotes |
, |
T3790 |
7328-7329 |
-RRB- |
denotes |
] |
T3791 |
7329-7331 |
, |
denotes |
, |
T3792 |
7331-7335 |
IN |
denotes |
into |
T3793 |
7336-7339 |
DT |
denotes |
the |
T3794 |
7345-7350 |
NN |
denotes |
locus |
T3795 |
7340-7344 |
NN |
denotes |
Er81 |
T3796 |
7351-7352 |
-LRB- |
denotes |
( |
T3797 |
7352-7358 |
NN |
denotes |
Figure |
T3798 |
7359-7360 |
CD |
denotes |
1 |
T3799 |
7360-7361 |
-RRB- |
denotes |
) |
T3800 |
7361-7362 |
. |
denotes |
. |
T3801 |
7362-7582 |
sentence |
denotes |
We found that in a luciferase-enzyme-based cell culture transfection assay, EWS-Pea3 showed stronger transactivation activity than Er81 or Pea3 (Figure 1J; data not shown), in agreement with previous studies [22,23,24]. |
T3802 |
7363-7365 |
PRP |
denotes |
We |
T3803 |
7366-7371 |
VBD |
denotes |
found |
T3804 |
7372-7376 |
IN |
denotes |
that |
T3805 |
7448-7454 |
VBD |
denotes |
showed |
T3806 |
7377-7379 |
IN |
denotes |
in |
T3807 |
7380-7381 |
DT |
denotes |
a |
T3808 |
7432-7437 |
NN |
denotes |
assay |
T3809 |
7382-7392 |
NN |
denotes |
luciferase |
T3810 |
7393-7399 |
NN |
denotes |
enzyme |
T3811 |
7392-7393 |
HYPH |
denotes |
- |
T3812 |
7400-7405 |
VBN |
denotes |
based |
T3813 |
7399-7400 |
HYPH |
denotes |
- |
T3814 |
7406-7410 |
NN |
denotes |
cell |
T3815 |
7411-7418 |
NN |
denotes |
culture |
T3816 |
7419-7431 |
NN |
denotes |
transfection |
T3817 |
7437-7439 |
, |
denotes |
, |
T3818 |
7439-7442 |
NN |
denotes |
EWS |
T3819 |
7443-7447 |
NN |
denotes |
Pea3 |
T3820 |
7442-7443 |
HYPH |
denotes |
- |
T3821 |
7455-7463 |
JJR |
denotes |
stronger |
T3822 |
7480-7488 |
NN |
denotes |
activity |
T3823 |
7464-7479 |
NN |
denotes |
transactivation |
T3824 |
7489-7493 |
IN |
denotes |
than |
T3825 |
7494-7498 |
NN |
denotes |
Er81 |
T3826 |
7499-7501 |
CC |
denotes |
or |
T3827 |
7502-7506 |
NN |
denotes |
Pea3 |
T3828 |
7507-7508 |
-LRB- |
denotes |
( |
T3829 |
7515-7517 |
NN |
denotes |
1J |
T3830 |
7508-7514 |
NN |
denotes |
Figure |
T3831 |
7517-7518 |
: |
denotes |
; |
T3832 |
7519-7523 |
NNS |
denotes |
data |
T3833 |
7528-7533 |
VBN |
denotes |
shown |
T3834 |
7524-7527 |
RB |
denotes |
not |
T3835 |
7533-7534 |
-RRB- |
denotes |
) |
T3836 |
7534-7536 |
, |
denotes |
, |
T3837 |
7536-7538 |
IN |
denotes |
in |
T3838 |
7539-7548 |
NN |
denotes |
agreement |
T3839 |
7549-7553 |
IN |
denotes |
with |
T3840 |
7554-7562 |
JJ |
denotes |
previous |
T3841 |
7563-7570 |
NNS |
denotes |
studies |
T3842 |
7571-7572 |
-LRB- |
denotes |
[ |
T3843 |
7578-7580 |
CD |
denotes |
24 |
T3844 |
7572-7574 |
CD |
denotes |
22 |
T3845 |
7574-7575 |
, |
denotes |
, |
T3846 |
7575-7577 |
CD |
denotes |
23 |
T3847 |
7577-7578 |
, |
denotes |
, |
T3848 |
7580-7581 |
-RRB- |
denotes |
] |
T3849 |
7581-7582 |
. |
denotes |
. |
T3850 |
7582-7752 |
sentence |
denotes |
Moreover, transactivation by EWS-Pea3 was abolished by mutation of ETS-binding sites in the reporter plasmid, demonstrating ETS-binding-site dependence (data not shown). |
T3851 |
7583-7591 |
RB |
denotes |
Moreover |
T3852 |
7625-7634 |
VBN |
denotes |
abolished |
T3853 |
7591-7593 |
, |
denotes |
, |
T3854 |
7593-7608 |
NN |
denotes |
transactivation |
T3855 |
7609-7611 |
IN |
denotes |
by |
T3856 |
7612-7615 |
NN |
denotes |
EWS |
T3857 |
7616-7620 |
NN |
denotes |
Pea3 |
T3858 |
7615-7616 |
HYPH |
denotes |
- |
T3859 |
7621-7624 |
VBD |
denotes |
was |
T3860 |
7635-7637 |
IN |
denotes |
by |
T3861 |
7638-7646 |
NN |
denotes |
mutation |
T3862 |
7647-7649 |
IN |
denotes |
of |
T3863 |
7650-7653 |
NN |
denotes |
ETS |
T3864 |
7654-7661 |
VBG |
denotes |
binding |
T3865 |
7653-7654 |
HYPH |
denotes |
- |
T3866 |
7662-7667 |
NNS |
denotes |
sites |
T3867 |
7668-7670 |
IN |
denotes |
in |
T3868 |
7671-7674 |
DT |
denotes |
the |
T3869 |
7684-7691 |
NN |
denotes |
plasmid |
T3870 |
7675-7683 |
NN |
denotes |
reporter |
T3871 |
7691-7693 |
, |
denotes |
, |
T3872 |
7693-7706 |
VBG |
denotes |
demonstrating |
T3873 |
7707-7710 |
NN |
denotes |
ETS |
T3874 |
7711-7718 |
VBG |
denotes |
binding |
T3875 |
7710-7711 |
HYPH |
denotes |
- |
T3876 |
7719-7723 |
NN |
denotes |
site |
T3877 |
7718-7719 |
HYPH |
denotes |
- |
T3878 |
7724-7734 |
NN |
denotes |
dependence |
T3879 |
7735-7736 |
-LRB- |
denotes |
( |
T3880 |
7745-7750 |
VBN |
denotes |
shown |
T3881 |
7736-7740 |
NNS |
denotes |
data |
T3882 |
7741-7744 |
RB |
denotes |
not |
T3883 |
7750-7751 |
-RRB- |
denotes |
) |
T3884 |
7751-7752 |
. |
denotes |
. |
T3885 |
7752-9744 |
sentence |
denotes |
Figure 1 Replacement of Er81 by EWS-Pea3
(A) Generation of Er81EWS-Pea3 mutant mice. Above is the organization of the Er81 genomic locus in the region targeted by homologous recombination in analogy to [14]. Exons 1–4 are shown as light blue boxes, and the Er81 start codon in exon 2 is indicated as ATG. The probe used to detect homologous recombination is shown as a grey box. Below is replacement of Er81 by EWS-Pea3 through the integration of EWS-Pea3 in frame with the endogenous start codon of the Er81 locus in exon 2 (in analogy to [14]).
(B) PCR and Southern blot analysis of Er81EWS-Pea3 wild-type (+/+), heterozygous (+/−), and homozygous (−/−) genomic DNA to detect the mutant allele. PCR primer pairs (EWS-Pea3ki) were used to detect specifically the recombined allele, and a primer pair in exon2 was used to detect the presence of the wild-type allele [14].
(C–E) Analysis of Er81 expression in lumbar DRG neurons of E16.5 wild-type (C), Er81−/− (D), and Er81EWS-Pea3/− (E) embryos. Inset in lower right corner of each panel shows Isl1 expression in the respective DRG.
(F–H) PV expression in lumbar DRG of E16.5 wild-type (F), Er81−/− (G), and Er81EWS-Pea3/− (H) embryos. Confocal scans were performed with equal gain intensity.
(J) Transcriptional transactivation of luciferase expression from a minimal reporter construct containing five consensus ETS DNA-binding sites ( GCCGGAAGC; [18,19]) and a minimal TK promoter upon transient transfection of Er81 (n ≥ 7; 3.03 ± 0.66) or EWS-Pea3 (n ≥ 7; 20.3 ± 2.7). Relative luciferase activity normalized to control (Con).
Scale bar: 80 μm. Expression of Er81 in DRG neurons of embryos containing integration of EWS-Pea3 in the Er81 locus (Er81EWS-Pea3/−) was abolished (Figure 1E), and the expression level of the calcium-binding protein Parvalbumin (PV) in proprioceptive afferents, which is decreased approximately 5- to 10-fold in Er81 mutants [14], was comparable to wild-type levels in Er81EWS-Pea3/− embryos (Figure 1F–1H). |
T3886 |
9355-9365 |
NN |
denotes |
Expression |
T3887 |
9474-9483 |
VBN |
denotes |
abolished |
T3888 |
9366-9368 |
IN |
denotes |
of |
T3889 |
9369-9373 |
NN |
denotes |
Er81 |
T3890 |
9374-9376 |
IN |
denotes |
in |
T3891 |
9377-9380 |
NN |
denotes |
DRG |
T3892 |
9381-9388 |
NNS |
denotes |
neurons |
T3893 |
9389-9391 |
IN |
denotes |
of |
T3894 |
9392-9399 |
NNS |
denotes |
embryos |
T3895 |
9400-9410 |
VBG |
denotes |
containing |
T3896 |
9411-9422 |
NN |
denotes |
integration |
T3897 |
9423-9425 |
IN |
denotes |
of |
T3898 |
9426-9429 |
NN |
denotes |
EWS |
T3899 |
9430-9434 |
NN |
denotes |
Pea3 |
T3900 |
9429-9430 |
HYPH |
denotes |
- |
T3901 |
9435-9437 |
IN |
denotes |
in |
T3902 |
9438-9441 |
DT |
denotes |
the |
T3903 |
9447-9452 |
NN |
denotes |
locus |
T3904 |
9442-9446 |
NN |
denotes |
Er81 |
T3905 |
9453-9454 |
-LRB- |
denotes |
( |
T3906 |
9454-9461 |
NN |
denotes |
Er81EWS |
T3907 |
9462-9466 |
NN |
denotes |
Pea3 |
T3908 |
9461-9462 |
HYPH |
denotes |
- |
T3909 |
9466-9467 |
HYPH |
denotes |
/ |
T3910 |
9467-9468 |
SYM |
denotes |
− |
T3911 |
9468-9469 |
-RRB- |
denotes |
) |
T3912 |
9470-9473 |
VBD |
denotes |
was |
T3913 |
9484-9485 |
-LRB- |
denotes |
( |
T3914 |
9492-9494 |
NN |
denotes |
1E |
T3915 |
9485-9491 |
NN |
denotes |
Figure |
T3916 |
9494-9495 |
-RRB- |
denotes |
) |
T3917 |
9495-9497 |
, |
denotes |
, |
T3918 |
9497-9500 |
CC |
denotes |
and |
T3919 |
9501-9504 |
DT |
denotes |
the |
T3920 |
9516-9521 |
NN |
denotes |
level |
T3921 |
9505-9515 |
NN |
denotes |
expression |
T3922 |
9668-9671 |
VBD |
denotes |
was |
T3923 |
9522-9524 |
IN |
denotes |
of |
T3924 |
9525-9528 |
DT |
denotes |
the |
T3925 |
9545-9552 |
NN |
denotes |
protein |
T3926 |
9529-9536 |
NN |
denotes |
calcium |
T3927 |
9537-9544 |
VBG |
denotes |
binding |
T3928 |
9536-9537 |
HYPH |
denotes |
- |
T3929 |
9553-9564 |
NN |
denotes |
Parvalbumin |
T3930 |
9565-9566 |
-LRB- |
denotes |
( |
T3931 |
9566-9568 |
NN |
denotes |
PV |
T3932 |
9568-9569 |
-RRB- |
denotes |
) |
T3933 |
9570-9572 |
IN |
denotes |
in |
T3934 |
9573-9587 |
JJ |
denotes |
proprioceptive |
T3935 |
9588-9597 |
NNS |
denotes |
afferents |
T3936 |
9597-9599 |
, |
denotes |
, |
T3937 |
9599-9604 |
WDT |
denotes |
which |
T3938 |
9608-9617 |
VBN |
denotes |
decreased |
T3939 |
9605-9607 |
VBZ |
denotes |
is |
T3940 |
9618-9631 |
RB |
denotes |
approximately |
T3941 |
9638-9640 |
CD |
denotes |
10 |
T3942 |
9632-9633 |
CD |
denotes |
5 |
T3943 |
9633-9634 |
HYPH |
denotes |
- |
T3944 |
9635-9637 |
IN |
denotes |
to |
T3945 |
9641-9645 |
RB |
denotes |
fold |
T3946 |
9640-9641 |
HYPH |
denotes |
- |
T3947 |
9646-9648 |
IN |
denotes |
in |
T3948 |
9649-9653 |
NN |
denotes |
Er81 |
T3949 |
9654-9661 |
NNS |
denotes |
mutants |
T3950 |
9662-9663 |
-LRB- |
denotes |
[ |
T3951 |
9663-9665 |
CD |
denotes |
14 |
T3952 |
9665-9666 |
-RRB- |
denotes |
] |
T3953 |
9666-9668 |
, |
denotes |
, |
T3954 |
9672-9682 |
JJ |
denotes |
comparable |
T3955 |
9683-9685 |
IN |
denotes |
to |
T3956 |
9686-9690 |
JJ |
denotes |
wild |
T3957 |
9691-9695 |
NN |
denotes |
type |
T3958 |
9690-9691 |
HYPH |
denotes |
- |
T3959 |
9696-9702 |
NNS |
denotes |
levels |
T3960 |
9703-9705 |
IN |
denotes |
in |
T3961 |
9706-9713 |
NN |
denotes |
Er81EWS |
T3962 |
9714-9718 |
NN |
denotes |
Pea3 |
T3963 |
9713-9714 |
HYPH |
denotes |
- |
T3964 |
9721-9728 |
NNS |
denotes |
embryos |
T3965 |
9718-9719 |
HYPH |
denotes |
/ |
T3966 |
9719-9720 |
SYM |
denotes |
− |
T3967 |
9729-9730 |
-LRB- |
denotes |
( |
T3968 |
9737-9739 |
NN |
denotes |
1F |
T3969 |
9730-9736 |
NN |
denotes |
Figure |
T3970 |
9739-9740 |
SYM |
denotes |
– |
T3971 |
9740-9742 |
NN |
denotes |
1H |
T3972 |
9742-9743 |
-RRB- |
denotes |
) |
T3973 |
9743-9744 |
. |
denotes |
. |
T3974 |
9744-10008 |
sentence |
denotes |
To further define DRG neuron differentiation in the presence of EWS-Pea3 in proprioceptive afferents in vivo, we assessed whether replacement of Er81 by EWS-Pea3 had an influence on neuronal survival or on the expression of proprioceptive-afferent-specific genes. |
T3975 |
9745-9747 |
TO |
denotes |
To |
T3976 |
9756-9762 |
VB |
denotes |
define |
T3977 |
9748-9755 |
RB |
denotes |
further |
T3978 |
9858-9866 |
VBD |
denotes |
assessed |
T3979 |
9763-9766 |
NN |
denotes |
DRG |
T3980 |
9767-9773 |
NN |
denotes |
neuron |
T3981 |
9774-9789 |
NN |
denotes |
differentiation |
T3982 |
9790-9792 |
IN |
denotes |
in |
T3983 |
9793-9796 |
DT |
denotes |
the |
T3984 |
9797-9805 |
NN |
denotes |
presence |
T3985 |
9806-9808 |
IN |
denotes |
of |
T3986 |
9809-9812 |
NN |
denotes |
EWS |
T3987 |
9813-9817 |
NN |
denotes |
Pea3 |
T3988 |
9812-9813 |
HYPH |
denotes |
- |
T3989 |
9818-9820 |
IN |
denotes |
in |
T3990 |
9821-9835 |
JJ |
denotes |
proprioceptive |
T3991 |
9836-9845 |
NNS |
denotes |
afferents |
T3992 |
9846-9848 |
FW |
denotes |
in |
T3993 |
9849-9853 |
FW |
denotes |
vivo |
T3994 |
9853-9855 |
, |
denotes |
, |
T3995 |
9855-9857 |
PRP |
denotes |
we |
T3996 |
9867-9874 |
IN |
denotes |
whether |
T3997 |
9907-9910 |
VBD |
denotes |
had |
T3998 |
9875-9886 |
NN |
denotes |
replacement |
T3999 |
9887-9889 |
IN |
denotes |
of |
T4000 |
9890-9894 |
NN |
denotes |
Er81 |
T4001 |
9895-9897 |
IN |
denotes |
by |
T4002 |
9898-9901 |
NN |
denotes |
EWS |
T4003 |
9902-9906 |
NN |
denotes |
Pea3 |
T4004 |
9901-9902 |
HYPH |
denotes |
- |
T4005 |
9911-9913 |
DT |
denotes |
an |
T4006 |
9914-9923 |
NN |
denotes |
influence |
T4007 |
9924-9926 |
IN |
denotes |
on |
T4008 |
9927-9935 |
JJ |
denotes |
neuronal |
T4009 |
9936-9944 |
NN |
denotes |
survival |
T4010 |
9945-9947 |
CC |
denotes |
or |
T4011 |
9948-9950 |
IN |
denotes |
on |
T4012 |
9951-9954 |
DT |
denotes |
the |
T4013 |
9955-9965 |
NN |
denotes |
expression |
T4014 |
9966-9968 |
IN |
denotes |
of |
T4015 |
9969-9983 |
JJ |
denotes |
proprioceptive |
T4016 |
9984-9992 |
JJ |
denotes |
afferent |
T4017 |
9983-9984 |
HYPH |
denotes |
- |
T4018 |
9993-10001 |
JJ |
denotes |
specific |
T4019 |
9992-9993 |
HYPH |
denotes |
- |
T4020 |
10002-10007 |
NNS |
denotes |
genes |
T4021 |
10007-10008 |
. |
denotes |
. |
T4022 |
10008-10335 |
sentence |
denotes |
Er81EWS-Pea3/− mice did not differ from wild-type in the number of proprioceptive afferent cell bodies within the DRG of L1 to L5, the expression of several genes normally expressed by proprioceptive afferents, and the lack of expression of genes not normally expressed in proprioceptive afferents (Figure S1; data not shown). |
T4023 |
10009-10016 |
NN |
denotes |
Er81EWS |
T4024 |
10017-10021 |
NN |
denotes |
Pea3 |
T4025 |
10016-10017 |
HYPH |
denotes |
- |
T4026 |
10024-10028 |
NNS |
denotes |
mice |
T4027 |
10021-10022 |
HYPH |
denotes |
/ |
T4028 |
10022-10023 |
SYM |
denotes |
− |
T4029 |
10037-10043 |
VB |
denotes |
differ |
T4030 |
10029-10032 |
VBD |
denotes |
did |
T4031 |
10033-10036 |
RB |
denotes |
not |
T4032 |
10044-10048 |
IN |
denotes |
from |
T4033 |
10049-10053 |
JJ |
denotes |
wild |
T4034 |
10054-10058 |
NN |
denotes |
type |
T4035 |
10053-10054 |
HYPH |
denotes |
- |
T4036 |
10059-10061 |
IN |
denotes |
in |
T4037 |
10062-10065 |
DT |
denotes |
the |
T4038 |
10066-10072 |
NN |
denotes |
number |
T4039 |
10073-10075 |
IN |
denotes |
of |
T4040 |
10076-10090 |
JJ |
denotes |
proprioceptive |
T4041 |
10105-10111 |
NNS |
denotes |
bodies |
T4042 |
10091-10099 |
JJ |
denotes |
afferent |
T4043 |
10100-10104 |
NN |
denotes |
cell |
T4044 |
10112-10118 |
IN |
denotes |
within |
T4045 |
10119-10122 |
DT |
denotes |
the |
T4046 |
10123-10126 |
NN |
denotes |
DRG |
T4047 |
10127-10129 |
IN |
denotes |
of |
T4048 |
10130-10132 |
NN |
denotes |
L1 |
T4049 |
10133-10135 |
IN |
denotes |
to |
T4050 |
10136-10138 |
NN |
denotes |
L5 |
T4051 |
10138-10140 |
, |
denotes |
, |
T4052 |
10140-10143 |
DT |
denotes |
the |
T4053 |
10144-10154 |
NN |
denotes |
expression |
T4054 |
10155-10157 |
IN |
denotes |
of |
T4055 |
10158-10165 |
JJ |
denotes |
several |
T4056 |
10166-10171 |
NNS |
denotes |
genes |
T4057 |
10172-10180 |
RB |
denotes |
normally |
T4058 |
10181-10190 |
VBN |
denotes |
expressed |
T4059 |
10191-10193 |
IN |
denotes |
by |
T4060 |
10194-10208 |
JJ |
denotes |
proprioceptive |
T4061 |
10209-10218 |
NNS |
denotes |
afferents |
T4062 |
10218-10220 |
, |
denotes |
, |
T4063 |
10220-10223 |
CC |
denotes |
and |
T4064 |
10224-10227 |
DT |
denotes |
the |
T4065 |
10228-10232 |
NN |
denotes |
lack |
T4066 |
10233-10235 |
IN |
denotes |
of |
T4067 |
10236-10246 |
NN |
denotes |
expression |
T4068 |
10247-10249 |
IN |
denotes |
of |
T4069 |
10250-10255 |
NNS |
denotes |
genes |
T4070 |
10256-10259 |
RB |
denotes |
not |
T4071 |
10269-10278 |
VBN |
denotes |
expressed |
T4072 |
10260-10268 |
RB |
denotes |
normally |
T4073 |
10279-10281 |
IN |
denotes |
in |
T4074 |
10282-10296 |
JJ |
denotes |
proprioceptive |
T4075 |
10297-10306 |
NNS |
denotes |
afferents |
T4076 |
10307-10308 |
-LRB- |
denotes |
( |
T4077 |
10315-10317 |
NN |
denotes |
S1 |
T4078 |
10308-10314 |
NN |
denotes |
Figure |
T4079 |
10317-10318 |
: |
denotes |
; |
T4080 |
10319-10323 |
NNS |
denotes |
data |
T4081 |
10328-10333 |
VBN |
denotes |
shown |
T4082 |
10324-10327 |
RB |
denotes |
not |
T4083 |
10333-10334 |
-RRB- |
denotes |
) |
T4084 |
10334-10335 |
. |
denotes |
. |
T4085 |
10335-10551 |
sentence |
denotes |
Together, these findings suggest that the expression of EWS-Pea3 from the normal time of onset mimics the function of Er81 as assessed by induction and maintenance of gene expression within proprioceptive afferents. |
T4086 |
10336-10344 |
RB |
denotes |
Together |
T4087 |
10361-10368 |
VBP |
denotes |
suggest |
T4088 |
10344-10346 |
, |
denotes |
, |
T4089 |
10346-10351 |
DT |
denotes |
these |
T4090 |
10352-10360 |
NNS |
denotes |
findings |
T4091 |
10369-10373 |
IN |
denotes |
that |
T4092 |
10431-10437 |
VBZ |
denotes |
mimics |
T4093 |
10374-10377 |
DT |
denotes |
the |
T4094 |
10378-10388 |
NN |
denotes |
expression |
T4095 |
10389-10391 |
IN |
denotes |
of |
T4096 |
10392-10395 |
NN |
denotes |
EWS |
T4097 |
10396-10400 |
NN |
denotes |
Pea3 |
T4098 |
10395-10396 |
HYPH |
denotes |
- |
T4099 |
10401-10405 |
IN |
denotes |
from |
T4100 |
10406-10409 |
DT |
denotes |
the |
T4101 |
10417-10421 |
NN |
denotes |
time |
T4102 |
10410-10416 |
JJ |
denotes |
normal |
T4103 |
10422-10424 |
IN |
denotes |
of |
T4104 |
10425-10430 |
NN |
denotes |
onset |
T4105 |
10438-10441 |
DT |
denotes |
the |
T4106 |
10442-10450 |
NN |
denotes |
function |
T4107 |
10451-10453 |
IN |
denotes |
of |
T4108 |
10454-10458 |
NN |
denotes |
Er81 |
T4109 |
10459-10461 |
IN |
denotes |
as |
T4110 |
10462-10470 |
VBN |
denotes |
assessed |
T4111 |
10471-10473 |
IN |
denotes |
by |
T4112 |
10474-10483 |
NN |
denotes |
induction |
T4113 |
10484-10487 |
CC |
denotes |
and |
T4114 |
10488-10499 |
NN |
denotes |
maintenance |
T4115 |
10500-10502 |
IN |
denotes |
of |
T4116 |
10503-10507 |
NN |
denotes |
gene |
T4117 |
10508-10518 |
NN |
denotes |
expression |
T4118 |
10519-10525 |
IN |
denotes |
within |
T4119 |
10526-10540 |
JJ |
denotes |
proprioceptive |
T4120 |
10541-10550 |
NNS |
denotes |
afferents |
T4121 |
10550-10551 |
. |
denotes |
. |
T4122 |
10551-10796 |
sentence |
denotes |
To determine the extent of rescue of Ia proprioceptive afferent projections into the ventral spinal cord of Er81 mutant mice achieved by expression of EWS-Pea3, we traced intraspinal afferent projections by axonal labeling of PV (Figure 2A–2C). |
T4123 |
10552-10554 |
TO |
denotes |
To |
T4124 |
10555-10564 |
VB |
denotes |
determine |
T4125 |
10716-10722 |
VBD |
denotes |
traced |
T4126 |
10565-10568 |
DT |
denotes |
the |
T4127 |
10569-10575 |
NN |
denotes |
extent |
T4128 |
10576-10578 |
IN |
denotes |
of |
T4129 |
10579-10585 |
NN |
denotes |
rescue |
T4130 |
10586-10588 |
IN |
denotes |
of |
T4131 |
10589-10591 |
NN |
denotes |
Ia |
T4132 |
10616-10627 |
NNS |
denotes |
projections |
T4133 |
10592-10606 |
JJ |
denotes |
proprioceptive |
T4134 |
10607-10615 |
JJ |
denotes |
afferent |
T4135 |
10628-10632 |
IN |
denotes |
into |
T4136 |
10633-10636 |
DT |
denotes |
the |
T4137 |
10652-10656 |
NN |
denotes |
cord |
T4138 |
10637-10644 |
JJ |
denotes |
ventral |
T4139 |
10645-10651 |
JJ |
denotes |
spinal |
T4140 |
10657-10659 |
IN |
denotes |
of |
T4141 |
10660-10664 |
NN |
denotes |
Er81 |
T4142 |
10672-10676 |
NNS |
denotes |
mice |
T4143 |
10665-10671 |
NN |
denotes |
mutant |
T4144 |
10677-10685 |
VBN |
denotes |
achieved |
T4145 |
10686-10688 |
IN |
denotes |
by |
T4146 |
10689-10699 |
NN |
denotes |
expression |
T4147 |
10700-10702 |
IN |
denotes |
of |
T4148 |
10703-10706 |
NN |
denotes |
EWS |
T4149 |
10707-10711 |
NN |
denotes |
Pea3 |
T4150 |
10706-10707 |
HYPH |
denotes |
- |
T4151 |
10711-10713 |
, |
denotes |
, |
T4152 |
10713-10715 |
PRP |
denotes |
we |
T4153 |
10723-10734 |
JJ |
denotes |
intraspinal |
T4154 |
10744-10755 |
NNS |
denotes |
projections |
T4155 |
10735-10743 |
JJ |
denotes |
afferent |
T4156 |
10756-10758 |
IN |
denotes |
by |
T4157 |
10759-10765 |
JJ |
denotes |
axonal |
T4158 |
10766-10774 |
NN |
denotes |
labeling |
T4159 |
10775-10777 |
IN |
denotes |
of |
T4160 |
10778-10780 |
NN |
denotes |
PV |
T4161 |
10781-10782 |
-LRB- |
denotes |
( |
T4162 |
10789-10791 |
NN |
denotes |
2A |
T4163 |
10782-10788 |
NN |
denotes |
Figure |
T4164 |
10791-10792 |
SYM |
denotes |
– |
T4165 |
10792-10794 |
NN |
denotes |
2C |
T4166 |
10794-10795 |
-RRB- |
denotes |
) |
T4167 |
10795-10796 |
. |
denotes |
. |
T4168 |
10796-11018 |
sentence |
denotes |
In addition, to analyze axon ingrowth independent of the level of PV expression in DRG neurons, we used anterograde labeling of afferent fibers by applying fluorescently labeled dextran to cut dorsal roots (Figure 2D–2F). |
T4169 |
10797-10799 |
IN |
denotes |
In |
T4170 |
10896-10900 |
VBD |
denotes |
used |
T4171 |
10800-10808 |
NN |
denotes |
addition |
T4172 |
10808-10810 |
, |
denotes |
, |
T4173 |
10810-10812 |
TO |
denotes |
to |
T4174 |
10813-10820 |
VB |
denotes |
analyze |
T4175 |
10821-10825 |
NN |
denotes |
axon |
T4176 |
10826-10834 |
NN |
denotes |
ingrowth |
T4177 |
10835-10846 |
RB |
denotes |
independent |
T4178 |
10847-10849 |
IN |
denotes |
of |
T4179 |
10850-10853 |
DT |
denotes |
the |
T4180 |
10854-10859 |
NN |
denotes |
level |
T4181 |
10860-10862 |
IN |
denotes |
of |
T4182 |
10863-10865 |
NN |
denotes |
PV |
T4183 |
10866-10876 |
NN |
denotes |
expression |
T4184 |
10877-10879 |
IN |
denotes |
in |
T4185 |
10880-10883 |
NN |
denotes |
DRG |
T4186 |
10884-10891 |
NNS |
denotes |
neurons |
T4187 |
10891-10893 |
, |
denotes |
, |
T4188 |
10893-10895 |
PRP |
denotes |
we |
T4189 |
10901-10912 |
JJ |
denotes |
anterograde |
T4190 |
10913-10921 |
NN |
denotes |
labeling |
T4191 |
10922-10924 |
IN |
denotes |
of |
T4192 |
10925-10933 |
JJ |
denotes |
afferent |
T4193 |
10934-10940 |
NNS |
denotes |
fibers |
T4194 |
10941-10943 |
IN |
denotes |
by |
T4195 |
10944-10952 |
VBG |
denotes |
applying |
T4196 |
10953-10966 |
RB |
denotes |
fluorescently |
T4197 |
10967-10974 |
VBN |
denotes |
labeled |
T4198 |
10975-10982 |
NN |
denotes |
dextran |
T4199 |
10983-10985 |
IN |
denotes |
to |
T4200 |
10986-10989 |
VBN |
denotes |
cut |
T4201 |
10997-11002 |
NNS |
denotes |
roots |
T4202 |
10990-10996 |
JJ |
denotes |
dorsal |
T4203 |
11003-11004 |
-LRB- |
denotes |
( |
T4204 |
11011-11013 |
NN |
denotes |
2D |
T4205 |
11004-11010 |
NN |
denotes |
Figure |
T4206 |
11013-11014 |
SYM |
denotes |
– |
T4207 |
11014-11016 |
NN |
denotes |
2F |
T4208 |
11016-11017 |
-RRB- |
denotes |
) |
T4209 |
11017-11018 |
. |
denotes |
. |
T4210 |
11018-11166 |
sentence |
denotes |
Using both assays, we found extensive rescue of the projections into the ventral horn of the spinal cord in Er81EWS-Pea3/− mice (Figure 2C and 2F). |
T4211 |
11019-11024 |
VBG |
denotes |
Using |
T4212 |
11041-11046 |
VBD |
denotes |
found |
T4213 |
11025-11029 |
DT |
denotes |
both |
T4214 |
11030-11036 |
NNS |
denotes |
assays |
T4215 |
11036-11038 |
, |
denotes |
, |
T4216 |
11038-11040 |
PRP |
denotes |
we |
T4217 |
11047-11056 |
JJ |
denotes |
extensive |
T4218 |
11057-11063 |
NN |
denotes |
rescue |
T4219 |
11064-11066 |
IN |
denotes |
of |
T4220 |
11067-11070 |
DT |
denotes |
the |
T4221 |
11071-11082 |
NNS |
denotes |
projections |
T4222 |
11083-11087 |
IN |
denotes |
into |
T4223 |
11088-11091 |
DT |
denotes |
the |
T4224 |
11100-11104 |
NN |
denotes |
horn |
T4225 |
11092-11099 |
JJ |
denotes |
ventral |
T4226 |
11105-11107 |
IN |
denotes |
of |
T4227 |
11108-11111 |
DT |
denotes |
the |
T4228 |
11119-11123 |
NN |
denotes |
cord |
T4229 |
11112-11118 |
JJ |
denotes |
spinal |
T4230 |
11124-11126 |
IN |
denotes |
in |
T4231 |
11127-11134 |
NN |
denotes |
Er81EWS |
T4232 |
11135-11139 |
NN |
denotes |
Pea3 |
T4233 |
11134-11135 |
HYPH |
denotes |
- |
T4234 |
11142-11146 |
NNS |
denotes |
mice |
T4235 |
11139-11140 |
HYPH |
denotes |
/ |
T4236 |
11140-11141 |
SYM |
denotes |
− |
T4237 |
11147-11148 |
-LRB- |
denotes |
( |
T4238 |
11155-11157 |
NN |
denotes |
2C |
T4239 |
11148-11154 |
NN |
denotes |
Figure |
T4240 |
11158-11161 |
CC |
denotes |
and |
T4241 |
11162-11164 |
NN |
denotes |
2F |
T4242 |
11164-11165 |
-RRB- |
denotes |
) |
T4243 |
11165-11166 |
. |
denotes |
. |
T4244 |
11166-11324 |
sentence |
denotes |
Within the ventral horn, Ia afferents in both wild-type and Er81EWS-Pea3/− mice formed vGlut1+ terminals that were absent in Er81 mutant mice (Figure 2G–2I). |
T4245 |
11167-11173 |
IN |
denotes |
Within |
T4246 |
11247-11253 |
VBD |
denotes |
formed |
T4247 |
11174-11177 |
DT |
denotes |
the |
T4248 |
11186-11190 |
NN |
denotes |
horn |
T4249 |
11178-11185 |
JJ |
denotes |
ventral |
T4250 |
11190-11192 |
, |
denotes |
, |
T4251 |
11192-11194 |
NN |
denotes |
Ia |
T4252 |
11195-11204 |
NNS |
denotes |
afferents |
T4253 |
11205-11207 |
IN |
denotes |
in |
T4254 |
11208-11212 |
CC |
denotes |
both |
T4255 |
11218-11222 |
NN |
denotes |
type |
T4256 |
11213-11217 |
JJ |
denotes |
wild |
T4257 |
11217-11218 |
HYPH |
denotes |
- |
T4258 |
11242-11246 |
NNS |
denotes |
mice |
T4259 |
11223-11226 |
CC |
denotes |
and |
T4260 |
11227-11234 |
NN |
denotes |
Er81EWS |
T4261 |
11235-11239 |
NN |
denotes |
Pea3 |
T4262 |
11234-11235 |
HYPH |
denotes |
- |
T4263 |
11239-11240 |
HYPH |
denotes |
/ |
T4264 |
11240-11241 |
SYM |
denotes |
− |
T4265 |
11254-11260 |
NN |
denotes |
vGlut1 |
T4266 |
11262-11271 |
NNS |
denotes |
terminals |
T4267 |
11260-11261 |
SYM |
denotes |
+ |
T4268 |
11272-11276 |
WDT |
denotes |
that |
T4269 |
11277-11281 |
VBD |
denotes |
were |
T4270 |
11282-11288 |
JJ |
denotes |
absent |
T4271 |
11289-11291 |
IN |
denotes |
in |
T4272 |
11292-11296 |
NN |
denotes |
Er81 |
T4273 |
11304-11308 |
NNS |
denotes |
mice |
T4274 |
11297-11303 |
NN |
denotes |
mutant |
T4275 |
11309-11310 |
-LRB- |
denotes |
( |
T4276 |
11317-11319 |
NN |
denotes |
2G |
T4277 |
11310-11316 |
NN |
denotes |
Figure |
T4278 |
11319-11320 |
SYM |
denotes |
– |
T4279 |
11320-11322 |
NN |
denotes |
2I |
T4280 |
11322-11323 |
-RRB- |
denotes |
) |
T4281 |
11323-11324 |
. |
denotes |
. |
T4282 |
11324-11577 |
sentence |
denotes |
To assess whether synapses between Ia afferents and motor neurons are functional in Er81EWS-Pea3/− mice, we performed intracellular recordings from identified quadriceps motor neurons after stimulation of nerves innervating the quadriceps muscle group. |
T4283 |
11325-11327 |
TO |
denotes |
To |
T4284 |
11328-11334 |
VB |
denotes |
assess |
T4285 |
11433-11442 |
VBD |
denotes |
performed |
T4286 |
11335-11342 |
IN |
denotes |
whether |
T4287 |
11391-11394 |
VBP |
denotes |
are |
T4288 |
11343-11351 |
NNS |
denotes |
synapses |
T4289 |
11352-11359 |
IN |
denotes |
between |
T4290 |
11360-11362 |
NN |
denotes |
Ia |
T4291 |
11363-11372 |
NNS |
denotes |
afferents |
T4292 |
11373-11376 |
CC |
denotes |
and |
T4293 |
11377-11382 |
NN |
denotes |
motor |
T4294 |
11383-11390 |
NNS |
denotes |
neurons |
T4295 |
11395-11405 |
JJ |
denotes |
functional |
T4296 |
11406-11408 |
IN |
denotes |
in |
T4297 |
11409-11416 |
NN |
denotes |
Er81EWS |
T4298 |
11417-11421 |
NN |
denotes |
Pea3 |
T4299 |
11416-11417 |
HYPH |
denotes |
- |
T4300 |
11424-11428 |
NNS |
denotes |
mice |
T4301 |
11421-11422 |
HYPH |
denotes |
/ |
T4302 |
11422-11423 |
SYM |
denotes |
− |
T4303 |
11428-11430 |
, |
denotes |
, |
T4304 |
11430-11432 |
PRP |
denotes |
we |
T4305 |
11443-11456 |
JJ |
denotes |
intracellular |
T4306 |
11457-11467 |
NNS |
denotes |
recordings |
T4307 |
11468-11472 |
IN |
denotes |
from |
T4308 |
11473-11483 |
VBN |
denotes |
identified |
T4309 |
11501-11508 |
NNS |
denotes |
neurons |
T4310 |
11484-11494 |
NNS |
denotes |
quadriceps |
T4311 |
11495-11500 |
NN |
denotes |
motor |
T4312 |
11509-11514 |
IN |
denotes |
after |
T4313 |
11515-11526 |
NN |
denotes |
stimulation |
T4314 |
11527-11529 |
IN |
denotes |
of |
T4315 |
11530-11536 |
NNS |
denotes |
nerves |
T4316 |
11537-11548 |
VBG |
denotes |
innervating |
T4317 |
11549-11552 |
DT |
denotes |
the |
T4318 |
11571-11576 |
NN |
denotes |
group |
T4319 |
11553-11563 |
NNS |
denotes |
quadriceps |
T4320 |
11564-11570 |
NN |
denotes |
muscle |
T4321 |
11576-11577 |
. |
denotes |
. |
T4322 |
11577-11794 |
sentence |
denotes |
We found no significant difference in the input amplitude to quadriceps motor neurons when comparing wild-type to Er81EWS-Pea3/− mice (Figure S2; wild-type, 10.6 ± 0.9 mV, n = 11; Er81EWS-Pea3/−, 10.9 ± 1 mV, n = 8). |
T4323 |
11578-11580 |
PRP |
denotes |
We |
T4324 |
11581-11586 |
VBD |
denotes |
found |
T4325 |
11587-11589 |
DT |
denotes |
no |
T4326 |
11602-11612 |
NN |
denotes |
difference |
T4327 |
11590-11601 |
JJ |
denotes |
significant |
T4328 |
11613-11615 |
IN |
denotes |
in |
T4329 |
11616-11619 |
DT |
denotes |
the |
T4330 |
11626-11635 |
NN |
denotes |
amplitude |
T4331 |
11620-11625 |
NN |
denotes |
input |
T4332 |
11636-11638 |
IN |
denotes |
to |
T4333 |
11639-11649 |
NNS |
denotes |
quadriceps |
T4334 |
11656-11663 |
NNS |
denotes |
neurons |
T4335 |
11650-11655 |
NN |
denotes |
motor |
T4336 |
11664-11668 |
WRB |
denotes |
when |
T4337 |
11669-11678 |
VBG |
denotes |
comparing |
T4338 |
11679-11683 |
JJ |
denotes |
wild |
T4339 |
11684-11688 |
NN |
denotes |
type |
T4340 |
11683-11684 |
HYPH |
denotes |
- |
T4341 |
11689-11691 |
IN |
denotes |
to |
T4342 |
11692-11699 |
NN |
denotes |
Er81EWS |
T4343 |
11700-11704 |
NN |
denotes |
Pea3 |
T4344 |
11699-11700 |
HYPH |
denotes |
- |
T4345 |
11704-11705 |
HYPH |
denotes |
/ |
T4346 |
11705-11706 |
SYM |
denotes |
− |
T4347 |
11707-11711 |
NNS |
denotes |
mice |
T4348 |
11712-11713 |
-LRB- |
denotes |
( |
T4349 |
11720-11722 |
NN |
denotes |
S2 |
T4350 |
11713-11719 |
NN |
denotes |
Figure |
T4351 |
11722-11723 |
: |
denotes |
; |
T4352 |
11724-11728 |
JJ |
denotes |
wild |
T4353 |
11729-11733 |
NN |
denotes |
type |
T4354 |
11728-11729 |
HYPH |
denotes |
- |
T4355 |
11754-11756 |
CD |
denotes |
11 |
T4356 |
11733-11735 |
, |
denotes |
, |
T4357 |
11735-11739 |
CD |
denotes |
10.6 |
T4358 |
11742-11745 |
CD |
denotes |
0.9 |
T4359 |
11740-11741 |
SYM |
denotes |
± |
T4360 |
11746-11748 |
NN |
denotes |
mV |
T4361 |
11748-11750 |
, |
denotes |
, |
T4362 |
11750-11751 |
NN |
denotes |
n |
T4363 |
11752-11753 |
SYM |
denotes |
= |
T4364 |
11756-11757 |
: |
denotes |
; |
T4365 |
11758-11765 |
NN |
denotes |
Er81EWS |
T4366 |
11766-11770 |
NN |
denotes |
Pea3 |
T4367 |
11765-11766 |
HYPH |
denotes |
- |
T4368 |
11791-11792 |
CD |
denotes |
8 |
T4369 |
11770-11771 |
HYPH |
denotes |
/ |
T4370 |
11771-11772 |
SYM |
denotes |
− |
T4371 |
11772-11774 |
, |
denotes |
, |
T4372 |
11774-11778 |
CD |
denotes |
10.9 |
T4373 |
11781-11782 |
CD |
denotes |
1 |
T4374 |
11779-11780 |
SYM |
denotes |
± |
T4375 |
11783-11785 |
NN |
denotes |
mV |
T4376 |
11785-11787 |
, |
denotes |
, |
T4377 |
11787-11788 |
NN |
denotes |
n |
T4378 |
11789-11790 |
SYM |
denotes |
= |
T4379 |
11792-11793 |
-RRB- |
denotes |
) |
T4380 |
11793-11794 |
. |
denotes |
. |
T4381 |
11794-12145 |
sentence |
denotes |
Together, these findings suggest that in the absence of Er81, EWS-Pea3 can direct the complex biological process of correct laminar termination within the ventral spinal cord and the formation of synapses with motor neurons (Figure 2J–2L), thus identifying an ETS transcription factor suitable for heterochronic expression experiments in DRG neurons. |
T4382 |
11795-11803 |
RB |
denotes |
Together |
T4383 |
11820-11827 |
VBP |
denotes |
suggest |
T4384 |
11803-11805 |
, |
denotes |
, |
T4385 |
11805-11810 |
DT |
denotes |
these |
T4386 |
11811-11819 |
NNS |
denotes |
findings |
T4387 |
11828-11832 |
IN |
denotes |
that |
T4388 |
11870-11876 |
VB |
denotes |
direct |
T4389 |
11833-11835 |
IN |
denotes |
in |
T4390 |
11836-11839 |
DT |
denotes |
the |
T4391 |
11840-11847 |
NN |
denotes |
absence |
T4392 |
11848-11850 |
IN |
denotes |
of |
T4393 |
11851-11855 |
NN |
denotes |
Er81 |
T4394 |
11855-11857 |
, |
denotes |
, |
T4395 |
11857-11860 |
NN |
denotes |
EWS |
T4396 |
11861-11865 |
NN |
denotes |
Pea3 |
T4397 |
11860-11861 |
HYPH |
denotes |
- |
T4398 |
11866-11869 |
MD |
denotes |
can |
T4399 |
11877-11880 |
DT |
denotes |
the |
T4400 |
11900-11907 |
NN |
denotes |
process |
T4401 |
11881-11888 |
JJ |
denotes |
complex |
T4402 |
11889-11899 |
JJ |
denotes |
biological |
T4403 |
11908-11910 |
IN |
denotes |
of |
T4404 |
11911-11918 |
JJ |
denotes |
correct |
T4405 |
11927-11938 |
NN |
denotes |
termination |
T4406 |
11919-11926 |
JJ |
denotes |
laminar |
T4407 |
11939-11945 |
IN |
denotes |
within |
T4408 |
11946-11949 |
DT |
denotes |
the |
T4409 |
11965-11969 |
NN |
denotes |
cord |
T4410 |
11950-11957 |
JJ |
denotes |
ventral |
T4411 |
11958-11964 |
JJ |
denotes |
spinal |
T4412 |
11970-11973 |
CC |
denotes |
and |
T4413 |
11974-11977 |
DT |
denotes |
the |
T4414 |
11978-11987 |
NN |
denotes |
formation |
T4415 |
11988-11990 |
IN |
denotes |
of |
T4416 |
11991-11999 |
NNS |
denotes |
synapses |
T4417 |
12000-12004 |
IN |
denotes |
with |
T4418 |
12005-12010 |
NN |
denotes |
motor |
T4419 |
12011-12018 |
NNS |
denotes |
neurons |
T4420 |
12019-12020 |
-LRB- |
denotes |
( |
T4421 |
12027-12029 |
NN |
denotes |
2J |
T4422 |
12020-12026 |
NN |
denotes |
Figure |
T4423 |
12029-12030 |
SYM |
denotes |
– |
T4424 |
12030-12032 |
NN |
denotes |
2L |
T4425 |
12032-12033 |
-RRB- |
denotes |
) |
T4426 |
12033-12035 |
, |
denotes |
, |
T4427 |
12035-12039 |
RB |
denotes |
thus |
T4428 |
12040-12051 |
VBG |
denotes |
identifying |
T4429 |
12052-12054 |
DT |
denotes |
an |
T4430 |
12073-12079 |
NN |
denotes |
factor |
T4431 |
12055-12058 |
NN |
denotes |
ETS |
T4432 |
12059-12072 |
NN |
denotes |
transcription |
T4433 |
12080-12088 |
JJ |
denotes |
suitable |
T4434 |
12089-12092 |
IN |
denotes |
for |
T4435 |
12093-12106 |
JJ |
denotes |
heterochronic |
T4436 |
12118-12129 |
NNS |
denotes |
experiments |
T4437 |
12107-12117 |
NN |
denotes |
expression |
T4438 |
12130-12132 |
IN |
denotes |
in |
T4439 |
12133-12136 |
NN |
denotes |
DRG |
T4440 |
12137-12144 |
NNS |
denotes |
neurons |
T4441 |
12144-12145 |
. |
denotes |
. |
T5254 |
13158-13168 |
JJ |
denotes |
Precocious |
T5255 |
13169-13179 |
NN |
denotes |
Expression |
T5256 |
13207-13212 |
VBZ |
denotes |
Leads |
T5257 |
13180-13182 |
IN |
denotes |
of |
T5258 |
13183-13186 |
NN |
denotes |
EWS |
T5259 |
13187-13191 |
NN |
denotes |
Pea3 |
T5260 |
13186-13187 |
HYPH |
denotes |
- |
T5261 |
13192-13194 |
IN |
denotes |
in |
T5262 |
13195-13198 |
NN |
denotes |
DRG |
T5263 |
13199-13206 |
NNS |
denotes |
Neurons |
T5264 |
13213-13215 |
IN |
denotes |
to |
T5265 |
13216-13222 |
JJ |
denotes |
Axonal |
T5266 |
13234-13241 |
NNS |
denotes |
Defects |
T5267 |
13223-13233 |
NN |
denotes |
Projection |
T5268 |
13241-13421 |
sentence |
denotes |
To address the consequences of precocious ETS signaling for proprioceptive afferent differentiation, we next expressed EWS-Pea3 in DRG neurons as soon as they became post-mitotic. |
T5269 |
13242-13244 |
TO |
denotes |
To |
T5270 |
13245-13252 |
VB |
denotes |
address |
T5271 |
13351-13360 |
VBD |
denotes |
expressed |
T5272 |
13253-13256 |
DT |
denotes |
the |
T5273 |
13257-13269 |
NNS |
denotes |
consequences |
T5274 |
13270-13272 |
IN |
denotes |
of |
T5275 |
13273-13283 |
JJ |
denotes |
precocious |
T5276 |
13288-13297 |
NN |
denotes |
signaling |
T5277 |
13284-13287 |
NN |
denotes |
ETS |
T5278 |
13298-13301 |
IN |
denotes |
for |
T5279 |
13302-13316 |
JJ |
denotes |
proprioceptive |
T5280 |
13317-13325 |
JJ |
denotes |
afferent |
T5281 |
13326-13341 |
NN |
denotes |
differentiation |
T5282 |
13341-13343 |
, |
denotes |
, |
T5283 |
13343-13345 |
PRP |
denotes |
we |
T5284 |
13346-13350 |
RB |
denotes |
next |
T5285 |
13361-13364 |
NN |
denotes |
EWS |
T5286 |
13365-13369 |
NN |
denotes |
Pea3 |
T5287 |
13364-13365 |
HYPH |
denotes |
- |
T5288 |
13370-13372 |
IN |
denotes |
in |
T5289 |
13373-13376 |
NN |
denotes |
DRG |
T5290 |
13377-13384 |
NNS |
denotes |
neurons |
T5291 |
13385-13387 |
RB |
denotes |
as |
T5292 |
13388-13392 |
RB |
denotes |
soon |
T5293 |
13393-13395 |
IN |
denotes |
as |
T5294 |
13401-13407 |
VBD |
denotes |
became |
T5295 |
13396-13400 |
PRP |
denotes |
they |
T5296 |
13408-13420 |
JJ |
denotes |
post-mitotic |
T5297 |
13420-13421 |
. |
denotes |
. |
T5298 |
13421-13560 |
sentence |
denotes |
We used a binary mouse genetic system based on Cre-recombinase-mediated excision of a transcriptional stop cassette flanked by loxP sites. |
T5299 |
13422-13424 |
PRP |
denotes |
We |
T5300 |
13425-13429 |
VBD |
denotes |
used |
T5301 |
13430-13431 |
DT |
denotes |
a |
T5302 |
13453-13459 |
NN |
denotes |
system |
T5303 |
13432-13438 |
JJ |
denotes |
binary |
T5304 |
13439-13444 |
NN |
denotes |
mouse |
T5305 |
13445-13452 |
JJ |
denotes |
genetic |
T5306 |
13460-13465 |
VBN |
denotes |
based |
T5307 |
13466-13468 |
IN |
denotes |
on |
T5308 |
13469-13472 |
NN |
denotes |
Cre |
T5309 |
13473-13484 |
NN |
denotes |
recombinase |
T5310 |
13472-13473 |
HYPH |
denotes |
- |
T5311 |
13485-13493 |
VBN |
denotes |
mediated |
T5312 |
13484-13485 |
HYPH |
denotes |
- |
T5313 |
13494-13502 |
NN |
denotes |
excision |
T5314 |
13503-13505 |
IN |
denotes |
of |
T5315 |
13506-13507 |
DT |
denotes |
a |
T5316 |
13529-13537 |
NN |
denotes |
cassette |
T5317 |
13508-13523 |
JJ |
denotes |
transcriptional |
T5318 |
13524-13528 |
NN |
denotes |
stop |
T5319 |
13538-13545 |
VBN |
denotes |
flanked |
T5320 |
13546-13548 |
IN |
denotes |
by |
T5321 |
13549-13553 |
NN |
denotes |
loxP |
T5322 |
13554-13559 |
NNS |
denotes |
sites |
T5323 |
13559-13560 |
. |
denotes |
. |
T5324 |
13560-13806 |
sentence |
denotes |
Targeting cassettes were integrated into the Tau locus to generate two strains of mice conditionally expressing either EWS-Pea3 or a membrane-targeted green fluorescent protein (mGFP) to trace axonal projections of DRG neurons (Figure S3; [25]). |
T5325 |
13561-13570 |
NN |
denotes |
Targeting |
T5326 |
13571-13580 |
NNS |
denotes |
cassettes |
T5327 |
13586-13596 |
VBN |
denotes |
integrated |
T5328 |
13581-13585 |
VBD |
denotes |
were |
T5329 |
13597-13601 |
IN |
denotes |
into |
T5330 |
13602-13605 |
DT |
denotes |
the |
T5331 |
13610-13615 |
NN |
denotes |
locus |
T5332 |
13606-13609 |
NN |
denotes |
Tau |
T5333 |
13616-13618 |
TO |
denotes |
to |
T5334 |
13619-13627 |
VB |
denotes |
generate |
T5335 |
13628-13631 |
CD |
denotes |
two |
T5336 |
13632-13639 |
NNS |
denotes |
strains |
T5337 |
13640-13642 |
IN |
denotes |
of |
T5338 |
13643-13647 |
NNS |
denotes |
mice |
T5339 |
13648-13661 |
RB |
denotes |
conditionally |
T5340 |
13662-13672 |
VBG |
denotes |
expressing |
T5341 |
13673-13679 |
CC |
denotes |
either |
T5342 |
13684-13688 |
NN |
denotes |
Pea3 |
T5343 |
13680-13683 |
NN |
denotes |
EWS |
T5344 |
13683-13684 |
HYPH |
denotes |
- |
T5345 |
13689-13691 |
CC |
denotes |
or |
T5346 |
13692-13693 |
DT |
denotes |
a |
T5347 |
13730-13737 |
NN |
denotes |
protein |
T5348 |
13694-13702 |
NN |
denotes |
membrane |
T5349 |
13703-13711 |
JJ |
denotes |
targeted |
T5350 |
13702-13703 |
HYPH |
denotes |
- |
T5351 |
13712-13717 |
JJ |
denotes |
green |
T5352 |
13718-13729 |
JJ |
denotes |
fluorescent |
T5353 |
13738-13739 |
-LRB- |
denotes |
( |
T5354 |
13739-13743 |
NN |
denotes |
mGFP |
T5355 |
13743-13744 |
-RRB- |
denotes |
) |
T5356 |
13745-13747 |
TO |
denotes |
to |
T5357 |
13748-13753 |
VB |
denotes |
trace |
T5358 |
13754-13760 |
JJ |
denotes |
axonal |
T5359 |
13761-13772 |
NNS |
denotes |
projections |
T5360 |
13773-13775 |
IN |
denotes |
of |
T5361 |
13776-13779 |
NN |
denotes |
DRG |
T5362 |
13780-13787 |
NNS |
denotes |
neurons |
T5363 |
13788-13789 |
-LRB- |
denotes |
( |
T5364 |
13801-13803 |
CD |
denotes |
25 |
T5365 |
13789-13795 |
NN |
denotes |
Figure |
T5366 |
13796-13798 |
NN |
denotes |
S3 |
T5367 |
13798-13799 |
: |
denotes |
; |
T5368 |
13800-13801 |
-LRB- |
denotes |
[ |
T5369 |
13803-13804 |
-RRB- |
denotes |
] |
T5370 |
13804-13805 |
-RRB- |
denotes |
) |
T5371 |
13805-13806 |
. |
denotes |
. |
T5372 |
13806-14058 |
sentence |
denotes |
Embryos positive for either Isl1Cre/+ and TauEWS-Pea3/+ or Isl1Cre/+ and TaumGFP/+ alleles showed efficient activation of the silent Tau allele in 95% or more of all DRG neurons, including proprioceptive afferents, at all segmental levels (Figure S3). |
T5373 |
13807-13814 |
NNS |
denotes |
Embryos |
T5374 |
13898-13904 |
VBD |
denotes |
showed |
T5375 |
13815-13823 |
JJ |
denotes |
positive |
T5376 |
13824-13827 |
IN |
denotes |
for |
T5377 |
13828-13834 |
CC |
denotes |
either |
T5378 |
13835-13842 |
NN |
denotes |
Isl1Cre |
T5379 |
13890-13897 |
NNS |
denotes |
alleles |
T5380 |
13842-13843 |
HYPH |
denotes |
/ |
T5381 |
13843-13844 |
SYM |
denotes |
+ |
T5382 |
13845-13848 |
CC |
denotes |
and |
T5383 |
13849-13855 |
NN |
denotes |
TauEWS |
T5384 |
13856-13860 |
NN |
denotes |
Pea3 |
T5385 |
13855-13856 |
HYPH |
denotes |
- |
T5386 |
13860-13861 |
HYPH |
denotes |
/ |
T5387 |
13861-13862 |
SYM |
denotes |
+ |
T5388 |
13863-13865 |
CC |
denotes |
or |
T5389 |
13866-13873 |
NN |
denotes |
Isl1Cre |
T5390 |
13873-13874 |
HYPH |
denotes |
/ |
T5391 |
13874-13875 |
SYM |
denotes |
+ |
T5392 |
13876-13879 |
CC |
denotes |
and |
T5393 |
13880-13887 |
NN |
denotes |
TaumGFP |
T5394 |
13887-13888 |
HYPH |
denotes |
/ |
T5395 |
13888-13889 |
SYM |
denotes |
+ |
T5396 |
13905-13914 |
JJ |
denotes |
efficient |
T5397 |
13915-13925 |
NN |
denotes |
activation |
T5398 |
13926-13928 |
IN |
denotes |
of |
T5399 |
13929-13932 |
DT |
denotes |
the |
T5400 |
13944-13950 |
NN |
denotes |
allele |
T5401 |
13933-13939 |
JJ |
denotes |
silent |
T5402 |
13940-13943 |
NN |
denotes |
Tau |
T5403 |
13951-13953 |
IN |
denotes |
in |
T5404 |
13954-13956 |
CD |
denotes |
95 |
T5405 |
13956-13957 |
NN |
denotes |
% |
T5406 |
13958-13960 |
CC |
denotes |
or |
T5407 |
13961-13965 |
JJR |
denotes |
more |
T5408 |
13966-13968 |
IN |
denotes |
of |
T5409 |
13969-13972 |
DT |
denotes |
all |
T5410 |
13977-13984 |
NNS |
denotes |
neurons |
T5411 |
13973-13976 |
NN |
denotes |
DRG |
T5412 |
13984-13986 |
, |
denotes |
, |
T5413 |
13986-13995 |
VBG |
denotes |
including |
T5414 |
13996-14010 |
JJ |
denotes |
proprioceptive |
T5415 |
14011-14020 |
NNS |
denotes |
afferents |
T5416 |
14020-14022 |
, |
denotes |
, |
T5417 |
14022-14024 |
IN |
denotes |
at |
T5418 |
14025-14028 |
DT |
denotes |
all |
T5419 |
14039-14045 |
NNS |
denotes |
levels |
T5420 |
14029-14038 |
JJ |
denotes |
segmental |
T5421 |
14046-14047 |
-LRB- |
denotes |
( |
T5422 |
14054-14056 |
NN |
denotes |
S3 |
T5423 |
14047-14053 |
NN |
denotes |
Figure |
T5424 |
14056-14057 |
-RRB- |
denotes |
) |
T5425 |
14057-14058 |
. |
denotes |
. |
T5426 |
14058-14431 |
sentence |
denotes |
We first assessed the influence of EWS-Pea3 expression in early post-mitotic DRG neurons on the establishment of afferent projections into the spinal cord using the TaumGFP/+ allele or a Thy1-promoter-driven synaptophysin green fluorescent protein (spGFP) with an expression profile restricted to DRG sensory neurons at embryonic day (E) 13.5 (Thy1spGFP; [25]) (Figure 3). |
T5427 |
14059-14061 |
PRP |
denotes |
We |
T5428 |
14068-14076 |
VBD |
denotes |
assessed |
T5429 |
14062-14067 |
RB |
denotes |
first |
T5430 |
14077-14080 |
DT |
denotes |
the |
T5431 |
14081-14090 |
NN |
denotes |
influence |
T5432 |
14091-14093 |
IN |
denotes |
of |
T5433 |
14094-14097 |
NN |
denotes |
EWS |
T5434 |
14098-14102 |
NN |
denotes |
Pea3 |
T5435 |
14097-14098 |
HYPH |
denotes |
- |
T5436 |
14103-14113 |
NN |
denotes |
expression |
T5437 |
14114-14116 |
IN |
denotes |
in |
T5438 |
14117-14122 |
JJ |
denotes |
early |
T5439 |
14140-14147 |
NNS |
denotes |
neurons |
T5440 |
14123-14135 |
JJ |
denotes |
post-mitotic |
T5441 |
14136-14139 |
NN |
denotes |
DRG |
T5442 |
14148-14150 |
IN |
denotes |
on |
T5443 |
14151-14154 |
DT |
denotes |
the |
T5444 |
14155-14168 |
NN |
denotes |
establishment |
T5445 |
14169-14171 |
IN |
denotes |
of |
T5446 |
14172-14180 |
JJ |
denotes |
afferent |
T5447 |
14181-14192 |
NNS |
denotes |
projections |
T5448 |
14193-14197 |
IN |
denotes |
into |
T5449 |
14198-14201 |
DT |
denotes |
the |
T5450 |
14209-14213 |
NN |
denotes |
cord |
T5451 |
14202-14208 |
JJ |
denotes |
spinal |
T5452 |
14214-14219 |
VBG |
denotes |
using |
T5453 |
14220-14223 |
DT |
denotes |
the |
T5454 |
14234-14240 |
NN |
denotes |
allele |
T5455 |
14224-14231 |
NN |
denotes |
TaumGFP |
T5456 |
14231-14232 |
HYPH |
denotes |
/ |
T5457 |
14232-14233 |
SYM |
denotes |
+ |
T5458 |
14241-14243 |
CC |
denotes |
or |
T5459 |
14244-14245 |
DT |
denotes |
a |
T5460 |
14299-14306 |
NN |
denotes |
protein |
T5461 |
14246-14250 |
NN |
denotes |
Thy1 |
T5462 |
14251-14259 |
NN |
denotes |
promoter |
T5463 |
14250-14251 |
HYPH |
denotes |
- |
T5464 |
14260-14266 |
VBN |
denotes |
driven |
T5465 |
14259-14260 |
HYPH |
denotes |
- |
T5466 |
14267-14280 |
NN |
denotes |
synaptophysin |
T5467 |
14281-14286 |
JJ |
denotes |
green |
T5468 |
14287-14298 |
JJ |
denotes |
fluorescent |
T5469 |
14307-14308 |
-LRB- |
denotes |
( |
T5470 |
14308-14313 |
NN |
denotes |
spGFP |
T5471 |
14313-14314 |
-RRB- |
denotes |
) |
T5472 |
14315-14319 |
IN |
denotes |
with |
T5473 |
14342-14352 |
VBN |
denotes |
restricted |
T5474 |
14320-14322 |
DT |
denotes |
an |
T5475 |
14334-14341 |
NN |
denotes |
profile |
T5476 |
14323-14333 |
NN |
denotes |
expression |
T5477 |
14353-14355 |
IN |
denotes |
to |
T5478 |
14356-14359 |
NN |
denotes |
DRG |
T5479 |
14368-14375 |
NNS |
denotes |
neurons |
T5480 |
14360-14367 |
JJ |
denotes |
sensory |
T5481 |
14376-14378 |
IN |
denotes |
at |
T5482 |
14379-14388 |
JJ |
denotes |
embryonic |
T5483 |
14389-14392 |
NN |
denotes |
day |
T5484 |
14393-14394 |
-LRB- |
denotes |
( |
T5485 |
14394-14395 |
NN |
denotes |
E |
T5486 |
14395-14396 |
-RRB- |
denotes |
) |
T5487 |
14397-14401 |
CD |
denotes |
13.5 |
T5488 |
14402-14403 |
-LRB- |
denotes |
( |
T5489 |
14421-14427 |
NN |
denotes |
Figure |
T5490 |
14403-14412 |
NN |
denotes |
Thy1spGFP |
T5491 |
14412-14413 |
: |
denotes |
; |
T5492 |
14414-14415 |
-LRB- |
denotes |
[ |
T5493 |
14415-14417 |
CD |
denotes |
25 |
T5494 |
14417-14418 |
-RRB- |
denotes |
] |
T5495 |
14418-14419 |
-RRB- |
denotes |
) |
T5496 |
14420-14421 |
-LRB- |
denotes |
( |
T5497 |
14428-14429 |
CD |
denotes |
3 |
T5498 |
14429-14430 |
-RRB- |
denotes |
) |
T5499 |
14430-14431 |
. |
denotes |
. |
T5500 |
14431-14768 |
sentence |
denotes |
In contrast to wild-type proprioceptive afferent projections (Figure 3A–3C), GFP+ sensory afferents in TauEWS-Pea3/+ Isl1Cre/+ embryos failed to invade the spinal cord and instead were found in an extreme lateral position at the dorsal root entry zone, a phenotype observed at least up to E18.5 (Figure 3A–3C and 3G–3I; data not shown). |
T5501 |
14432-14434 |
IN |
denotes |
In |
T5502 |
14567-14573 |
VBD |
denotes |
failed |
T5503 |
14435-14443 |
NN |
denotes |
contrast |
T5504 |
14444-14446 |
IN |
denotes |
to |
T5505 |
14447-14451 |
JJ |
denotes |
wild |
T5506 |
14452-14456 |
NN |
denotes |
type |
T5507 |
14451-14452 |
HYPH |
denotes |
- |
T5508 |
14481-14492 |
NNS |
denotes |
projections |
T5509 |
14457-14471 |
JJ |
denotes |
proprioceptive |
T5510 |
14472-14480 |
JJ |
denotes |
afferent |
T5511 |
14493-14494 |
-LRB- |
denotes |
( |
T5512 |
14501-14503 |
NN |
denotes |
3A |
T5513 |
14494-14500 |
NN |
denotes |
Figure |
T5514 |
14503-14504 |
SYM |
denotes |
– |
T5515 |
14504-14506 |
NN |
denotes |
3C |
T5516 |
14506-14507 |
-RRB- |
denotes |
) |
T5517 |
14507-14509 |
, |
denotes |
, |
T5518 |
14509-14512 |
NN |
denotes |
GFP |
T5519 |
14522-14531 |
NNS |
denotes |
afferents |
T5520 |
14512-14513 |
SYM |
denotes |
+ |
T5521 |
14514-14521 |
JJ |
denotes |
sensory |
T5522 |
14532-14534 |
IN |
denotes |
in |
T5523 |
14535-14541 |
NN |
denotes |
TauEWS |
T5524 |
14542-14546 |
NN |
denotes |
Pea3 |
T5525 |
14541-14542 |
HYPH |
denotes |
- |
T5526 |
14559-14566 |
NNS |
denotes |
embryos |
T5527 |
14546-14547 |
HYPH |
denotes |
/ |
T5528 |
14547-14548 |
SYM |
denotes |
+ |
T5529 |
14549-14556 |
NN |
denotes |
Isl1Cre |
T5530 |
14556-14557 |
HYPH |
denotes |
/ |
T5531 |
14557-14558 |
SYM |
denotes |
+ |
T5532 |
14574-14576 |
TO |
denotes |
to |
T5533 |
14577-14583 |
VB |
denotes |
invade |
T5534 |
14584-14587 |
DT |
denotes |
the |
T5535 |
14595-14599 |
NN |
denotes |
cord |
T5536 |
14588-14594 |
JJ |
denotes |
spinal |
T5537 |
14600-14603 |
CC |
denotes |
and |
T5538 |
14604-14611 |
RB |
denotes |
instead |
T5539 |
14617-14622 |
VBN |
denotes |
found |
T5540 |
14612-14616 |
VBD |
denotes |
were |
T5541 |
14623-14625 |
IN |
denotes |
in |
T5542 |
14626-14628 |
DT |
denotes |
an |
T5543 |
14645-14653 |
NN |
denotes |
position |
T5544 |
14629-14636 |
RB |
denotes |
extreme |
T5545 |
14637-14644 |
JJ |
denotes |
lateral |
T5546 |
14654-14656 |
IN |
denotes |
at |
T5547 |
14657-14660 |
DT |
denotes |
the |
T5548 |
14679-14683 |
NN |
denotes |
zone |
T5549 |
14661-14667 |
JJ |
denotes |
dorsal |
T5550 |
14668-14672 |
NN |
denotes |
root |
T5551 |
14673-14678 |
NN |
denotes |
entry |
T5552 |
14683-14685 |
, |
denotes |
, |
T5553 |
14685-14686 |
DT |
denotes |
a |
T5554 |
14687-14696 |
NN |
denotes |
phenotype |
T5555 |
14697-14705 |
VBN |
denotes |
observed |
T5556 |
14706-14708 |
RB |
denotes |
at |
T5557 |
14709-14714 |
RBS |
denotes |
least |
T5558 |
14715-14717 |
IN |
denotes |
up |
T5559 |
14718-14720 |
IN |
denotes |
to |
T5560 |
14721-14726 |
NN |
denotes |
E18.5 |
T5561 |
14727-14728 |
-LRB- |
denotes |
( |
T5562 |
14735-14737 |
NN |
denotes |
3A |
T5563 |
14728-14734 |
NN |
denotes |
Figure |
T5564 |
14737-14738 |
SYM |
denotes |
– |
T5565 |
14738-14740 |
NN |
denotes |
3C |
T5566 |
14741-14744 |
CC |
denotes |
and |
T5567 |
14745-14747 |
NN |
denotes |
3G |
T5568 |
14747-14748 |
SYM |
denotes |
– |
T5569 |
14748-14750 |
NN |
denotes |
3I |
T5570 |
14750-14751 |
: |
denotes |
; |
T5571 |
14752-14756 |
NNS |
denotes |
data |
T5572 |
14761-14766 |
VBN |
denotes |
shown |
T5573 |
14757-14760 |
RB |
denotes |
not |
T5574 |
14766-14767 |
-RRB- |
denotes |
) |
T5575 |
14767-14768 |
. |
denotes |
. |
T5576 |
14768-14991 |
sentence |
denotes |
We next visualized the path of sensory afferent projections towards the dorsal root entry zone in TauEWS-Pea3/+ Isl1Cre/+ embryos by injecting fluorescently labeled dextran into an individual DRG (L3; n = 3; Figure 3M–3Q). |
T5577 |
14769-14771 |
PRP |
denotes |
We |
T5578 |
14777-14787 |
VBD |
denotes |
visualized |
T5579 |
14772-14776 |
RB |
denotes |
next |
T5580 |
14788-14791 |
DT |
denotes |
the |
T5581 |
14792-14796 |
NN |
denotes |
path |
T5582 |
14797-14799 |
IN |
denotes |
of |
T5583 |
14800-14807 |
JJ |
denotes |
sensory |
T5584 |
14808-14816 |
JJ |
denotes |
afferent |
T5585 |
14817-14828 |
NNS |
denotes |
projections |
T5586 |
14829-14836 |
IN |
denotes |
towards |
T5587 |
14837-14840 |
DT |
denotes |
the |
T5588 |
14859-14863 |
NN |
denotes |
zone |
T5589 |
14841-14847 |
JJ |
denotes |
dorsal |
T5590 |
14848-14852 |
NN |
denotes |
root |
T5591 |
14853-14858 |
NN |
denotes |
entry |
T5592 |
14864-14866 |
IN |
denotes |
in |
T5593 |
14867-14873 |
NN |
denotes |
TauEWS |
T5594 |
14881-14888 |
NN |
denotes |
Isl1Cre |
T5595 |
14873-14874 |
HYPH |
denotes |
- |
T5596 |
14874-14878 |
NN |
denotes |
Pea3 |
T5597 |
14878-14879 |
HYPH |
denotes |
/ |
T5598 |
14879-14880 |
SYM |
denotes |
+ |
T5599 |
14891-14898 |
NNS |
denotes |
embryos |
T5600 |
14888-14889 |
HYPH |
denotes |
/ |
T5601 |
14889-14890 |
SYM |
denotes |
+ |
T5602 |
14899-14901 |
IN |
denotes |
by |
T5603 |
14902-14911 |
VBG |
denotes |
injecting |
T5604 |
14912-14925 |
RB |
denotes |
fluorescently |
T5605 |
14926-14933 |
VBN |
denotes |
labeled |
T5606 |
14934-14941 |
NN |
denotes |
dextran |
T5607 |
14942-14946 |
IN |
denotes |
into |
T5608 |
14947-14949 |
DT |
denotes |
an |
T5609 |
14961-14964 |
NN |
denotes |
DRG |
T5610 |
14950-14960 |
JJ |
denotes |
individual |
T5611 |
14965-14966 |
-LRB- |
denotes |
( |
T5612 |
14984-14986 |
NN |
denotes |
3M |
T5613 |
14966-14968 |
NN |
denotes |
L3 |
T5614 |
14968-14969 |
: |
denotes |
; |
T5615 |
14970-14971 |
NN |
denotes |
n |
T5616 |
14974-14975 |
CD |
denotes |
3 |
T5617 |
14972-14973 |
SYM |
denotes |
= |
T5618 |
14975-14976 |
: |
denotes |
; |
T5619 |
14977-14983 |
NN |
denotes |
Figure |
T5620 |
14986-14987 |
SYM |
denotes |
– |
T5621 |
14987-14989 |
NN |
denotes |
3Q |
T5622 |
14989-14990 |
-RRB- |
denotes |
) |
T5623 |
14990-14991 |
. |
denotes |
. |
T5624 |
14991-15208 |
sentence |
denotes |
Sensory afferents in E13.5 wild-type embryos bifurcated at their lateral spinal entry point, and projected rostrally and caudally over six or more segmental levels while gradually approaching the midline (Figure 3M). |
T5625 |
14992-14999 |
JJ |
denotes |
Sensory |
T5626 |
15000-15009 |
NNS |
denotes |
afferents |
T5627 |
15037-15047 |
VBN |
denotes |
bifurcated |
T5628 |
15010-15012 |
IN |
denotes |
in |
T5629 |
15013-15018 |
NN |
denotes |
E13.5 |
T5630 |
15029-15036 |
NNS |
denotes |
embryos |
T5631 |
15019-15023 |
JJ |
denotes |
wild |
T5632 |
15024-15028 |
NN |
denotes |
type |
T5633 |
15023-15024 |
HYPH |
denotes |
- |
T5634 |
15048-15050 |
IN |
denotes |
at |
T5635 |
15051-15056 |
PRP$ |
denotes |
their |
T5636 |
15078-15083 |
NN |
denotes |
point |
T5637 |
15057-15064 |
JJ |
denotes |
lateral |
T5638 |
15065-15071 |
JJ |
denotes |
spinal |
T5639 |
15072-15077 |
NN |
denotes |
entry |
T5640 |
15083-15085 |
, |
denotes |
, |
T5641 |
15085-15088 |
CC |
denotes |
and |
T5642 |
15089-15098 |
VBN |
denotes |
projected |
T5643 |
15099-15108 |
RB |
denotes |
rostrally |
T5644 |
15109-15112 |
CC |
denotes |
and |
T5645 |
15113-15121 |
RB |
denotes |
caudally |
T5646 |
15122-15126 |
IN |
denotes |
over |
T5647 |
15127-15130 |
CD |
denotes |
six |
T5648 |
15149-15155 |
NNS |
denotes |
levels |
T5649 |
15131-15133 |
CC |
denotes |
or |
T5650 |
15134-15138 |
JJR |
denotes |
more |
T5651 |
15139-15148 |
JJ |
denotes |
segmental |
T5652 |
15156-15161 |
IN |
denotes |
while |
T5653 |
15172-15183 |
VBG |
denotes |
approaching |
T5654 |
15162-15171 |
RB |
denotes |
gradually |
T5655 |
15184-15187 |
DT |
denotes |
the |
T5656 |
15188-15195 |
NN |
denotes |
midline |
T5657 |
15196-15197 |
-LRB- |
denotes |
( |
T5658 |
15204-15206 |
NN |
denotes |
3M |
T5659 |
15197-15203 |
NN |
denotes |
Figure |
T5660 |
15206-15207 |
-RRB- |
denotes |
) |
T5661 |
15207-15208 |
. |
denotes |
. |
T5662 |
15208-15400 |
sentence |
denotes |
Sensory afferents in TauEWS-Pea3/+ Isl1Cre/+ embryos also bifurcated at the entry point, although approximately 5% of afferent fibers continued to grow towards the midline (Figure 3O and 3Q). |
T5663 |
15209-15216 |
JJ |
denotes |
Sensory |
T5664 |
15217-15226 |
NNS |
denotes |
afferents |
T5665 |
15267-15277 |
VBN |
denotes |
bifurcated |
T5666 |
15227-15229 |
IN |
denotes |
in |
T5667 |
15230-15236 |
NN |
denotes |
TauEWS |
T5668 |
15244-15251 |
NN |
denotes |
Isl1Cre |
T5669 |
15236-15237 |
HYPH |
denotes |
- |
T5670 |
15237-15241 |
NN |
denotes |
Pea3 |
T5671 |
15241-15242 |
HYPH |
denotes |
/ |
T5672 |
15242-15243 |
SYM |
denotes |
+ |
T5673 |
15254-15261 |
NNS |
denotes |
embryos |
T5674 |
15251-15252 |
HYPH |
denotes |
/ |
T5675 |
15252-15253 |
SYM |
denotes |
+ |
T5676 |
15262-15266 |
RB |
denotes |
also |
T5677 |
15278-15280 |
IN |
denotes |
at |
T5678 |
15281-15284 |
DT |
denotes |
the |
T5679 |
15291-15296 |
NN |
denotes |
point |
T5680 |
15285-15290 |
NN |
denotes |
entry |
T5681 |
15296-15298 |
, |
denotes |
, |
T5682 |
15298-15306 |
IN |
denotes |
although |
T5683 |
15343-15352 |
VBD |
denotes |
continued |
T5684 |
15307-15320 |
RB |
denotes |
approximately |
T5685 |
15321-15322 |
CD |
denotes |
5 |
T5686 |
15322-15323 |
NN |
denotes |
% |
T5687 |
15324-15326 |
IN |
denotes |
of |
T5688 |
15327-15335 |
JJ |
denotes |
afferent |
T5689 |
15336-15342 |
NNS |
denotes |
fibers |
T5690 |
15353-15355 |
TO |
denotes |
to |
T5691 |
15356-15360 |
VB |
denotes |
grow |
T5692 |
15361-15368 |
IN |
denotes |
towards |
T5693 |
15369-15372 |
DT |
denotes |
the |
T5694 |
15373-15380 |
NN |
denotes |
midline |
T5695 |
15381-15382 |
-LRB- |
denotes |
( |
T5696 |
15389-15391 |
NN |
denotes |
3O |
T5697 |
15382-15388 |
NN |
denotes |
Figure |
T5698 |
15392-15395 |
CC |
denotes |
and |
T5699 |
15396-15398 |
NN |
denotes |
3Q |
T5700 |
15398-15399 |
-RRB- |
denotes |
) |
T5701 |
15399-15400 |
. |
denotes |
. |
T5702 |
15400-15665 |
sentence |
denotes |
While rostro-caudal projections were present in TauEWS-Pea3/+ Isl1Cre/+ embryos, afferent fibers failed to approach the midline at distal segments and continued to occupy an extreme lateral position (Figure 3O), consistent with the analysis of transverse sections. |
T5703 |
15401-15406 |
IN |
denotes |
While |
T5704 |
15433-15437 |
VBD |
denotes |
were |
T5705 |
15407-15413 |
JJ |
denotes |
rostro |
T5706 |
15414-15420 |
JJ |
denotes |
caudal |
T5707 |
15413-15414 |
HYPH |
denotes |
- |
T5708 |
15421-15432 |
NNS |
denotes |
projections |
T5709 |
15498-15504 |
VBD |
denotes |
failed |
T5710 |
15438-15445 |
JJ |
denotes |
present |
T5711 |
15446-15448 |
IN |
denotes |
in |
T5712 |
15449-15455 |
NN |
denotes |
TauEWS |
T5713 |
15463-15470 |
NN |
denotes |
Isl1Cre |
T5714 |
15455-15456 |
HYPH |
denotes |
- |
T5715 |
15456-15460 |
NN |
denotes |
Pea3 |
T5716 |
15460-15461 |
HYPH |
denotes |
/ |
T5717 |
15461-15462 |
SYM |
denotes |
+ |
T5718 |
15473-15480 |
NNS |
denotes |
embryos |
T5719 |
15470-15471 |
HYPH |
denotes |
/ |
T5720 |
15471-15472 |
SYM |
denotes |
+ |
T5721 |
15480-15482 |
, |
denotes |
, |
T5722 |
15482-15490 |
JJ |
denotes |
afferent |
T5723 |
15491-15497 |
NNS |
denotes |
fibers |
T5724 |
15505-15507 |
TO |
denotes |
to |
T5725 |
15508-15516 |
VB |
denotes |
approach |
T5726 |
15517-15520 |
DT |
denotes |
the |
T5727 |
15521-15528 |
NN |
denotes |
midline |
T5728 |
15529-15531 |
IN |
denotes |
at |
T5729 |
15532-15538 |
JJ |
denotes |
distal |
T5730 |
15539-15547 |
NNS |
denotes |
segments |
T5731 |
15548-15551 |
CC |
denotes |
and |
T5732 |
15552-15561 |
VBD |
denotes |
continued |
T5733 |
15562-15564 |
TO |
denotes |
to |
T5734 |
15565-15571 |
VB |
denotes |
occupy |
T5735 |
15572-15574 |
DT |
denotes |
an |
T5736 |
15591-15599 |
NN |
denotes |
position |
T5737 |
15575-15582 |
RB |
denotes |
extreme |
T5738 |
15583-15590 |
JJ |
denotes |
lateral |
T5739 |
15600-15601 |
-LRB- |
denotes |
( |
T5740 |
15608-15610 |
NN |
denotes |
3O |
T5741 |
15601-15607 |
NN |
denotes |
Figure |
T5742 |
15610-15611 |
-RRB- |
denotes |
) |
T5743 |
15611-15613 |
, |
denotes |
, |
T5744 |
15613-15623 |
JJ |
denotes |
consistent |
T5745 |
15624-15628 |
IN |
denotes |
with |
T5746 |
15629-15632 |
DT |
denotes |
the |
T5747 |
15633-15641 |
NN |
denotes |
analysis |
T5748 |
15642-15644 |
IN |
denotes |
of |
T5749 |
15645-15655 |
JJ |
denotes |
transverse |
T5750 |
15656-15664 |
NNS |
denotes |
sections |
T5751 |
15664-15665 |
. |
denotes |
. |
T5752 |
15665-17461 |
sentence |
denotes |
Figure 3 Defects in the Establishment of Sensory Afferent Projections upon Precocious Expression of EWS-Pea3 in DRG Neurons
(A–C and G–I) Visualization of sensory afferent projections (green) into the spinal cord of wild-type (A–C) and TauEWS-Pea3/+ Isl1Cre/+ (G–I) embryos at E13.5 (A, C, G, and I) and E16.5 (B and H) by Cre-recombinase-mediated activation of mGFP expression from the Tau locus (A, B, G, and H) or by a Thy1spGFP transgene (C and I; [25]). Grey arrows indicate normal pattern of afferent projections into the spinal cord, whereas red arrows show aberrant accumulation of sensory afferents at the lateral edge of the spinal cord in TauEWS-Pea3/+ Isl1Cre/+ embryos.
(D–F and J–L) Analysis of sensory afferent projections (green) into the skin (D and J) or muscle (E and K; red, α-Bungarotoxin, BTX) of wild-type (D–F) and TauEWS-Pea3/+ Isl1Cre/+ (J–L) embryos at E16.5 by Cre-recombinase-mediated activation of mGFP (D, E, J, and K) expression from the Tau locus. (F and L) show Egr3 expression in intrafusal muscle fibers using in situ hybridization (consecutive sections to [E and K] are shown).
(M–Q) Analysis of bifurcation of sensory afferent projections towards the spinal cord in E13.5 wild-type (M) and TauEWS-Pea3/+ Isl1Cre/+ (O and Q) embryos after injection of fluorescently labeled dextran (green) into one DRG (lumbar level L3). Confocal scanning plane for (M and O) is schematically illustrated in (N). Inset in (O) is also shown at a deeper confocal scanning plane (P and Q) to visualize aberrant axonal projections.
Scale bar: (A and G), 60 μm; (B and H), 80 μm; (C and I), 100 μm; (D and J), 160 μm; (E, F, K, and L), 70 μm; (M, O, and Q), 240 μm. We next examined the establishment of peripheral projections upon precocious EWS-Pea3 expression in DRG neurons. |
T5753 |
17349-17351 |
PRP |
denotes |
We |
T5754 |
17357-17365 |
VBD |
denotes |
examined |
T5755 |
17352-17356 |
RB |
denotes |
next |
T5756 |
17366-17369 |
DT |
denotes |
the |
T5757 |
17370-17383 |
NN |
denotes |
establishment |
T5758 |
17384-17386 |
IN |
denotes |
of |
T5759 |
17387-17397 |
JJ |
denotes |
peripheral |
T5760 |
17398-17409 |
NNS |
denotes |
projections |
T5761 |
17410-17414 |
IN |
denotes |
upon |
T5762 |
17415-17425 |
JJ |
denotes |
precocious |
T5763 |
17435-17445 |
NN |
denotes |
expression |
T5764 |
17426-17429 |
NN |
denotes |
EWS |
T5765 |
17430-17434 |
NN |
denotes |
Pea3 |
T5766 |
17429-17430 |
HYPH |
denotes |
- |
T5767 |
17446-17448 |
IN |
denotes |
in |
T5768 |
17449-17452 |
NN |
denotes |
DRG |
T5769 |
17453-17460 |
NNS |
denotes |
neurons |
T5770 |
17460-17461 |
. |
denotes |
. |
T5771 |
17461-17670 |
sentence |
denotes |
While sensory axons in TauEWS-Pea3/+ Isl1Cre/+ embryos reached the skin and established major nerve trunks by E16.5, only rudimentary sensory axon branching was established within the skin (Figure 3D and 3J). |
T5772 |
17462-17467 |
IN |
denotes |
While |
T5773 |
17517-17524 |
VBD |
denotes |
reached |
T5774 |
17468-17475 |
JJ |
denotes |
sensory |
T5775 |
17476-17481 |
NNS |
denotes |
axons |
T5776 |
17482-17484 |
IN |
denotes |
in |
T5777 |
17485-17491 |
NN |
denotes |
TauEWS |
T5778 |
17499-17506 |
NN |
denotes |
Isl1Cre |
T5779 |
17491-17492 |
HYPH |
denotes |
- |
T5780 |
17492-17496 |
NN |
denotes |
Pea3 |
T5781 |
17496-17497 |
HYPH |
denotes |
/ |
T5782 |
17497-17498 |
SYM |
denotes |
+ |
T5783 |
17509-17516 |
NNS |
denotes |
embryos |
T5784 |
17506-17507 |
HYPH |
denotes |
/ |
T5785 |
17507-17508 |
SYM |
denotes |
+ |
T5786 |
17623-17634 |
VBN |
denotes |
established |
T5787 |
17525-17528 |
DT |
denotes |
the |
T5788 |
17529-17533 |
NN |
denotes |
skin |
T5789 |
17534-17537 |
CC |
denotes |
and |
T5790 |
17538-17549 |
VBN |
denotes |
established |
T5791 |
17550-17555 |
JJ |
denotes |
major |
T5792 |
17562-17568 |
NNS |
denotes |
trunks |
T5793 |
17556-17561 |
NN |
denotes |
nerve |
T5794 |
17569-17571 |
IN |
denotes |
by |
T5795 |
17572-17577 |
NN |
denotes |
E16.5 |
T5796 |
17577-17579 |
, |
denotes |
, |
T5797 |
17579-17583 |
RB |
denotes |
only |
T5798 |
17609-17618 |
NN |
denotes |
branching |
T5799 |
17584-17595 |
JJ |
denotes |
rudimentary |
T5800 |
17596-17603 |
JJ |
denotes |
sensory |
T5801 |
17604-17608 |
NN |
denotes |
axon |
T5802 |
17619-17622 |
VBD |
denotes |
was |
T5803 |
17635-17641 |
IN |
denotes |
within |
T5804 |
17642-17645 |
DT |
denotes |
the |
T5805 |
17646-17650 |
NN |
denotes |
skin |
T5806 |
17651-17652 |
-LRB- |
denotes |
( |
T5807 |
17659-17661 |
NN |
denotes |
3D |
T5808 |
17652-17658 |
NN |
denotes |
Figure |
T5809 |
17662-17665 |
CC |
denotes |
and |
T5810 |
17666-17668 |
NN |
denotes |
3J |
T5811 |
17668-17669 |
-RRB- |
denotes |
) |
T5812 |
17669-17670 |
. |
denotes |
. |
T5813 |
17670-17973 |
sentence |
denotes |
In addition, there was a significant reduction in the number of muscle spindles in TauEWS-Pea3/+ Isl1Cre/+ embryos (approximately 25% of wild-type complement; n = 3) as assessed by innervation and expression of genes specific for intrafusal muscle fibers such as Egr3 (Figure 3E, 3F, 3K, and 3L; [26]). |
T5814 |
17671-17673 |
IN |
denotes |
In |
T5815 |
17690-17693 |
VBD |
denotes |
was |
T5816 |
17674-17682 |
NN |
denotes |
addition |
T5817 |
17682-17684 |
, |
denotes |
, |
T5818 |
17684-17689 |
EX |
denotes |
there |
T5819 |
17694-17695 |
DT |
denotes |
a |
T5820 |
17708-17717 |
NN |
denotes |
reduction |
T5821 |
17696-17707 |
JJ |
denotes |
significant |
T5822 |
17718-17720 |
IN |
denotes |
in |
T5823 |
17721-17724 |
DT |
denotes |
the |
T5824 |
17725-17731 |
NN |
denotes |
number |
T5825 |
17732-17734 |
IN |
denotes |
of |
T5826 |
17735-17741 |
NN |
denotes |
muscle |
T5827 |
17742-17750 |
NNS |
denotes |
spindles |
T5828 |
17751-17753 |
IN |
denotes |
in |
T5829 |
17754-17760 |
NN |
denotes |
TauEWS |
T5830 |
17768-17775 |
NN |
denotes |
Isl1Cre |
T5831 |
17760-17761 |
HYPH |
denotes |
- |
T5832 |
17761-17765 |
NN |
denotes |
Pea3 |
T5833 |
17765-17766 |
HYPH |
denotes |
/ |
T5834 |
17766-17767 |
SYM |
denotes |
+ |
T5835 |
17778-17785 |
NNS |
denotes |
embryos |
T5836 |
17775-17776 |
HYPH |
denotes |
/ |
T5837 |
17776-17777 |
SYM |
denotes |
+ |
T5838 |
17786-17787 |
-LRB- |
denotes |
( |
T5839 |
17803-17804 |
NN |
denotes |
% |
T5840 |
17787-17800 |
RB |
denotes |
approximately |
T5841 |
17801-17803 |
CD |
denotes |
25 |
T5842 |
17805-17807 |
IN |
denotes |
of |
T5843 |
17808-17812 |
JJ |
denotes |
wild |
T5844 |
17813-17817 |
NN |
denotes |
type |
T5845 |
17812-17813 |
HYPH |
denotes |
- |
T5846 |
17818-17828 |
NN |
denotes |
complement |
T5847 |
17828-17829 |
: |
denotes |
; |
T5848 |
17830-17831 |
NN |
denotes |
n |
T5849 |
17834-17835 |
CD |
denotes |
3 |
T5850 |
17832-17833 |
SYM |
denotes |
= |
T5851 |
17835-17836 |
-RRB- |
denotes |
) |
T5852 |
17837-17839 |
IN |
denotes |
as |
T5853 |
17840-17848 |
VBN |
denotes |
assessed |
T5854 |
17849-17851 |
IN |
denotes |
by |
T5855 |
17852-17863 |
NN |
denotes |
innervation |
T5856 |
17864-17867 |
CC |
denotes |
and |
T5857 |
17868-17878 |
NN |
denotes |
expression |
T5858 |
17879-17881 |
IN |
denotes |
of |
T5859 |
17882-17887 |
NNS |
denotes |
genes |
T5860 |
17888-17896 |
JJ |
denotes |
specific |
T5861 |
17897-17900 |
IN |
denotes |
for |
T5862 |
17901-17911 |
JJ |
denotes |
intrafusal |
T5863 |
17919-17925 |
NNS |
denotes |
fibers |
T5864 |
17912-17918 |
NN |
denotes |
muscle |
T5865 |
17926-17930 |
JJ |
denotes |
such |
T5866 |
17931-17933 |
IN |
denotes |
as |
T5867 |
17934-17938 |
NN |
denotes |
Egr3 |
T5868 |
17939-17940 |
-LRB- |
denotes |
( |
T5869 |
17968-17970 |
CD |
denotes |
26 |
T5870 |
17940-17946 |
NN |
denotes |
Figure |
T5871 |
17947-17949 |
NN |
denotes |
3E |
T5872 |
17949-17951 |
, |
denotes |
, |
T5873 |
17951-17953 |
NN |
denotes |
3F |
T5874 |
17953-17955 |
, |
denotes |
, |
T5875 |
17955-17957 |
NN |
denotes |
3K |
T5876 |
17957-17959 |
, |
denotes |
, |
T5877 |
17959-17962 |
CC |
denotes |
and |
T5878 |
17963-17965 |
NN |
denotes |
3L |
T5879 |
17965-17966 |
: |
denotes |
; |
T5880 |
17967-17968 |
-LRB- |
denotes |
[ |
T5881 |
17970-17971 |
-RRB- |
denotes |
] |
T5882 |
17971-17972 |
-RRB- |
denotes |
) |
T5883 |
17972-17973 |
. |
denotes |
. |
T5884 |
17973-18293 |
sentence |
denotes |
In summary, whereas isochronic expression of EWS-Pea3 promoted the establishment of proprioceptive afferent projections into the ventral spinal cord, precocious expression of the same ETS signaling factor in DRG neurons interfered with establishment of projections into the spinal cord as well as to peripheral targets. |
T5885 |
17974-17976 |
IN |
denotes |
In |
T5886 |
18194-18204 |
VBD |
denotes |
interfered |
T5887 |
17977-17984 |
NN |
denotes |
summary |
T5888 |
17984-17986 |
, |
denotes |
, |
T5889 |
17986-17993 |
IN |
denotes |
whereas |
T5890 |
18028-18036 |
VBD |
denotes |
promoted |
T5891 |
17994-18004 |
JJ |
denotes |
isochronic |
T5892 |
18005-18015 |
NN |
denotes |
expression |
T5893 |
18016-18018 |
IN |
denotes |
of |
T5894 |
18019-18022 |
NN |
denotes |
EWS |
T5895 |
18023-18027 |
NN |
denotes |
Pea3 |
T5896 |
18022-18023 |
HYPH |
denotes |
- |
T5897 |
18037-18040 |
DT |
denotes |
the |
T5898 |
18041-18054 |
NN |
denotes |
establishment |
T5899 |
18055-18057 |
IN |
denotes |
of |
T5900 |
18058-18072 |
JJ |
denotes |
proprioceptive |
T5901 |
18082-18093 |
NNS |
denotes |
projections |
T5902 |
18073-18081 |
JJ |
denotes |
afferent |
T5903 |
18094-18098 |
IN |
denotes |
into |
T5904 |
18099-18102 |
DT |
denotes |
the |
T5905 |
18118-18122 |
NN |
denotes |
cord |
T5906 |
18103-18110 |
JJ |
denotes |
ventral |
T5907 |
18111-18117 |
JJ |
denotes |
spinal |
T5908 |
18122-18124 |
, |
denotes |
, |
T5909 |
18124-18134 |
JJ |
denotes |
precocious |
T5910 |
18135-18145 |
NN |
denotes |
expression |
T5911 |
18146-18148 |
IN |
denotes |
of |
T5912 |
18149-18152 |
DT |
denotes |
the |
T5913 |
18172-18178 |
NN |
denotes |
factor |
T5914 |
18153-18157 |
JJ |
denotes |
same |
T5915 |
18158-18161 |
NN |
denotes |
ETS |
T5916 |
18162-18171 |
NN |
denotes |
signaling |
T5917 |
18179-18181 |
IN |
denotes |
in |
T5918 |
18182-18185 |
NN |
denotes |
DRG |
T5919 |
18186-18193 |
NNS |
denotes |
neurons |
T5920 |
18205-18209 |
IN |
denotes |
with |
T5921 |
18210-18223 |
NN |
denotes |
establishment |
T5922 |
18224-18226 |
IN |
denotes |
of |
T5923 |
18227-18238 |
NNS |
denotes |
projections |
T5924 |
18239-18243 |
IN |
denotes |
into |
T5925 |
18244-18247 |
DT |
denotes |
the |
T5926 |
18255-18259 |
NN |
denotes |
cord |
T5927 |
18248-18254 |
JJ |
denotes |
spinal |
T5928 |
18260-18262 |
RB |
denotes |
as |
T5929 |
18268-18270 |
IN |
denotes |
as |
T5930 |
18263-18267 |
RB |
denotes |
well |
T5931 |
18271-18273 |
IN |
denotes |
to |
T5932 |
18274-18284 |
JJ |
denotes |
peripheral |
T5933 |
18285-18292 |
NNS |
denotes |
targets |
T5934 |
18292-18293 |
. |
denotes |
. |
T6718 |
18636-18646 |
JJ |
denotes |
precocious |
T6663 |
18295-18305 |
JJ |
denotes |
Precocious |
T6664 |
18315-18325 |
NN |
denotes |
Expression |
T6665 |
18306-18309 |
NN |
denotes |
EWS |
T6666 |
18310-18314 |
NN |
denotes |
Pea3 |
T6667 |
18309-18310 |
HYPH |
denotes |
- |
T6668 |
18326-18334 |
VBZ |
denotes |
Promotes |
T6669 |
18335-18347 |
NN |
denotes |
Neurotrophin |
T6670 |
18348-18359 |
JJ |
denotes |
Independent |
T6671 |
18347-18348 |
HYPH |
denotes |
- |
T6672 |
18360-18368 |
NN |
denotes |
Survival |
T6673 |
18369-18372 |
CC |
denotes |
and |
T6674 |
18373-18380 |
NN |
denotes |
Neurite |
T6675 |
18381-18390 |
NN |
denotes |
Outgrowth |
T6676 |
18390-18588 |
sentence |
denotes |
To begin to address the cellular and molecular mechanisms involved in the distinct biological actions of EWS-Pea3 at different developmental stages, we first turned to in vitro culture experiments. |
T6677 |
18391-18393 |
TO |
denotes |
To |
T6678 |
18394-18399 |
VB |
denotes |
begin |
T6679 |
18549-18555 |
VBD |
denotes |
turned |
T6680 |
18400-18402 |
TO |
denotes |
to |
T6681 |
18403-18410 |
VB |
denotes |
address |
T6682 |
18411-18414 |
DT |
denotes |
the |
T6683 |
18438-18448 |
NNS |
denotes |
mechanisms |
T6684 |
18415-18423 |
JJ |
denotes |
cellular |
T6685 |
18424-18427 |
CC |
denotes |
and |
T6686 |
18428-18437 |
JJ |
denotes |
molecular |
T6687 |
18449-18457 |
VBN |
denotes |
involved |
T6688 |
18458-18460 |
IN |
denotes |
in |
T6689 |
18461-18464 |
DT |
denotes |
the |
T6690 |
18485-18492 |
NNS |
denotes |
actions |
T6691 |
18465-18473 |
JJ |
denotes |
distinct |
T6692 |
18474-18484 |
JJ |
denotes |
biological |
T6693 |
18493-18495 |
IN |
denotes |
of |
T6694 |
18496-18499 |
NN |
denotes |
EWS |
T6695 |
18500-18504 |
NN |
denotes |
Pea3 |
T6696 |
18499-18500 |
HYPH |
denotes |
- |
T6697 |
18505-18507 |
IN |
denotes |
at |
T6698 |
18508-18517 |
JJ |
denotes |
different |
T6699 |
18532-18538 |
NNS |
denotes |
stages |
T6700 |
18518-18531 |
JJ |
denotes |
developmental |
T6701 |
18538-18540 |
, |
denotes |
, |
T6702 |
18540-18542 |
PRP |
denotes |
we |
T6703 |
18543-18548 |
RB |
denotes |
first |
T6704 |
18556-18558 |
IN |
denotes |
to |
T6705 |
18559-18561 |
FW |
denotes |
in |
T6706 |
18562-18567 |
FW |
denotes |
vitro |
T6707 |
18576-18587 |
NNS |
denotes |
experiments |
T6708 |
18568-18575 |
NN |
denotes |
culture |
T6709 |
18587-18588 |
. |
denotes |
. |
T6710 |
18588-18855 |
sentence |
denotes |
These experiments permit assessment of whether precocious ETS transcription factor signaling influences neuronal survival and in vitro neurite outgrowth of DRG neurons, two parameters prominently influenced by target-derived neurotrophic factors and their receptors. |
T6711 |
18589-18594 |
DT |
denotes |
These |
T6712 |
18595-18606 |
NNS |
denotes |
experiments |
T6713 |
18607-18613 |
VBP |
denotes |
permit |
T6714 |
18614-18624 |
NN |
denotes |
assessment |
T6715 |
18625-18627 |
IN |
denotes |
of |
T6716 |
18628-18635 |
IN |
denotes |
whether |
T6717 |
18682-18692 |
VBZ |
denotes |
influences |
T6719 |
18672-18681 |
NN |
denotes |
signaling |
T6720 |
18647-18650 |
NN |
denotes |
ETS |
T6721 |
18651-18664 |
NN |
denotes |
transcription |
T6722 |
18665-18671 |
NN |
denotes |
factor |
T6723 |
18693-18701 |
JJ |
denotes |
neuronal |
T6724 |
18702-18710 |
NN |
denotes |
survival |
T6725 |
18711-18714 |
CC |
denotes |
and |
T6726 |
18715-18717 |
FW |
denotes |
in |
T6727 |
18718-18723 |
FW |
denotes |
vitro |
T6728 |
18732-18741 |
NN |
denotes |
outgrowth |
T6729 |
18724-18731 |
NN |
denotes |
neurite |
T6730 |
18742-18744 |
IN |
denotes |
of |
T6731 |
18745-18748 |
NN |
denotes |
DRG |
T6732 |
18749-18756 |
NNS |
denotes |
neurons |
T6733 |
18756-18758 |
, |
denotes |
, |
T6734 |
18758-18761 |
CD |
denotes |
two |
T6735 |
18762-18772 |
NNS |
denotes |
parameters |
T6736 |
18773-18784 |
RB |
denotes |
prominently |
T6737 |
18785-18795 |
VBN |
denotes |
influenced |
T6738 |
18796-18798 |
IN |
denotes |
by |
T6739 |
18799-18805 |
NN |
denotes |
target |
T6740 |
18806-18813 |
VBN |
denotes |
derived |
T6741 |
18805-18806 |
HYPH |
denotes |
- |
T6742 |
18827-18834 |
NNS |
denotes |
factors |
T6743 |
18814-18826 |
JJ |
denotes |
neurotrophic |
T6744 |
18835-18838 |
CC |
denotes |
and |
T6745 |
18839-18844 |
PRP$ |
denotes |
their |
T6746 |
18845-18854 |
NNS |
denotes |
receptors |
T6747 |
18854-18855 |
. |
denotes |
. |
T6748 |
18855-19105 |
sentence |
denotes |
We cultured E13.5 whole DRG explants from wild-type and TauEWS-Pea3/+ Isl1Cre/+ embryos in the presence of NGF or NT-3 or in the absence of neurotrophins and analyzed neuronal survival and neurite outgrowth on matrigel substrate after 48 h in vitro. |
T6749 |
18856-18858 |
PRP |
denotes |
We |
T6750 |
18859-18867 |
VBD |
denotes |
cultured |
T6751 |
18868-18873 |
NN |
denotes |
E13.5 |
T6752 |
18884-18892 |
NNS |
denotes |
explants |
T6753 |
18874-18879 |
JJ |
denotes |
whole |
T6754 |
18880-18883 |
NN |
denotes |
DRG |
T6755 |
18893-18897 |
IN |
denotes |
from |
T6756 |
18898-18902 |
JJ |
denotes |
wild |
T6757 |
18903-18907 |
NN |
denotes |
type |
T6758 |
18902-18903 |
HYPH |
denotes |
- |
T6759 |
18936-18943 |
NNS |
denotes |
embryos |
T6760 |
18908-18911 |
CC |
denotes |
and |
T6761 |
18912-18918 |
NN |
denotes |
TauEWS |
T6762 |
18926-18933 |
NN |
denotes |
Isl1Cre |
T6763 |
18918-18919 |
HYPH |
denotes |
- |
T6764 |
18919-18923 |
NN |
denotes |
Pea3 |
T6765 |
18923-18924 |
HYPH |
denotes |
/ |
T6766 |
18924-18925 |
SYM |
denotes |
+ |
T6767 |
18933-18934 |
HYPH |
denotes |
/ |
T6768 |
18934-18935 |
SYM |
denotes |
+ |
T6769 |
18944-18946 |
IN |
denotes |
in |
T6770 |
18947-18950 |
DT |
denotes |
the |
T6771 |
18951-18959 |
NN |
denotes |
presence |
T6772 |
18960-18962 |
IN |
denotes |
of |
T6773 |
18963-18966 |
NN |
denotes |
NGF |
T6774 |
18967-18969 |
CC |
denotes |
or |
T6775 |
18970-18972 |
NN |
denotes |
NT |
T6776 |
18972-18973 |
HYPH |
denotes |
- |
T6777 |
18973-18974 |
CD |
denotes |
3 |
T6778 |
18975-18977 |
CC |
denotes |
or |
T6779 |
18978-18980 |
IN |
denotes |
in |
T6780 |
18981-18984 |
DT |
denotes |
the |
T6781 |
18985-18992 |
NN |
denotes |
absence |
T6782 |
18993-18995 |
IN |
denotes |
of |
T6783 |
18996-19009 |
NNS |
denotes |
neurotrophins |
T6784 |
19010-19013 |
CC |
denotes |
and |
T6785 |
19014-19022 |
VBN |
denotes |
analyzed |
T6786 |
19023-19031 |
JJ |
denotes |
neuronal |
T6787 |
19032-19040 |
NN |
denotes |
survival |
T6788 |
19041-19044 |
CC |
denotes |
and |
T6789 |
19045-19052 |
NN |
denotes |
neurite |
T6790 |
19053-19062 |
NN |
denotes |
outgrowth |
T6791 |
19063-19065 |
IN |
denotes |
on |
T6792 |
19066-19074 |
NN |
denotes |
matrigel |
T6793 |
19075-19084 |
NN |
denotes |
substrate |
T6794 |
19085-19090 |
IN |
denotes |
after |
T6795 |
19091-19093 |
CD |
denotes |
48 |
T6796 |
19094-19095 |
NN |
denotes |
h |
T6797 |
19096-19098 |
FW |
denotes |
in |
T6798 |
19099-19104 |
FW |
denotes |
vitro |
T6799 |
19104-19105 |
. |
denotes |
. |
T6800 |
19105-19188 |
sentence |
denotes |
Without neurotrophic support, very few wild-type DRG neurons survived (Figure 4A). |
T6801 |
19106-19113 |
IN |
denotes |
Without |
T6802 |
19167-19175 |
VBD |
denotes |
survived |
T6803 |
19114-19126 |
JJ |
denotes |
neurotrophic |
T6804 |
19127-19134 |
NN |
denotes |
support |
T6805 |
19134-19136 |
, |
denotes |
, |
T6806 |
19136-19140 |
RB |
denotes |
very |
T6807 |
19141-19144 |
JJ |
denotes |
few |
T6808 |
19159-19166 |
NNS |
denotes |
neurons |
T6809 |
19145-19149 |
JJ |
denotes |
wild |
T6810 |
19150-19154 |
NN |
denotes |
type |
T6811 |
19149-19150 |
HYPH |
denotes |
- |
T6812 |
19155-19158 |
NN |
denotes |
DRG |
T6813 |
19176-19177 |
-LRB- |
denotes |
( |
T6814 |
19184-19186 |
NN |
denotes |
4A |
T6815 |
19177-19183 |
NN |
denotes |
Figure |
T6816 |
19186-19187 |
-RRB- |
denotes |
) |
T6817 |
19187-19188 |
. |
denotes |
. |
T6818 |
19188-19299 |
sentence |
denotes |
In contrast, culturing wild-type DRG with neurotrophic factors led to neuronal survival and neurite outgrowth. |
T6819 |
19189-19191 |
IN |
denotes |
In |
T6820 |
19252-19255 |
VBD |
denotes |
led |
T6821 |
19192-19200 |
NN |
denotes |
contrast |
T6822 |
19200-19202 |
, |
denotes |
, |
T6823 |
19202-19211 |
VBG |
denotes |
culturing |
T6824 |
19212-19216 |
JJ |
denotes |
wild |
T6825 |
19217-19221 |
NN |
denotes |
type |
T6826 |
19216-19217 |
HYPH |
denotes |
- |
T6827 |
19222-19225 |
NN |
denotes |
DRG |
T6828 |
19226-19230 |
IN |
denotes |
with |
T6829 |
19231-19243 |
JJ |
denotes |
neurotrophic |
T6830 |
19244-19251 |
NNS |
denotes |
factors |
T6831 |
19256-19258 |
IN |
denotes |
to |
T6832 |
19259-19267 |
JJ |
denotes |
neuronal |
T6833 |
19268-19276 |
NN |
denotes |
survival |
T6834 |
19277-19280 |
CC |
denotes |
and |
T6835 |
19281-19288 |
NN |
denotes |
neurite |
T6836 |
19289-19298 |
NN |
denotes |
outgrowth |
T6837 |
19298-19299 |
. |
denotes |
. |
T6838 |
19299-19619 |
sentence |
denotes |
Addition of NGF, which supports survival of cutaneous afferents, resulted in straight and unbranched neurite outgrowth (Figure 4B), while cultures grown in the presence of NT-3, which supports survival of proprioceptive afferents, resulted in a highly branched neurite outgrowth pattern after 48 h in vitro (Figure 4C). |
T6839 |
19300-19308 |
NN |
denotes |
Addition |
T6840 |
19365-19373 |
VBD |
denotes |
resulted |
T6841 |
19309-19311 |
IN |
denotes |
of |
T6842 |
19312-19315 |
NN |
denotes |
NGF |
T6843 |
19315-19317 |
, |
denotes |
, |
T6844 |
19317-19322 |
WDT |
denotes |
which |
T6845 |
19323-19331 |
VBZ |
denotes |
supports |
T6846 |
19332-19340 |
NN |
denotes |
survival |
T6847 |
19341-19343 |
IN |
denotes |
of |
T6848 |
19344-19353 |
JJ |
denotes |
cutaneous |
T6849 |
19354-19363 |
NNS |
denotes |
afferents |
T6850 |
19363-19365 |
, |
denotes |
, |
T6851 |
19374-19376 |
IN |
denotes |
in |
T6852 |
19377-19385 |
JJ |
denotes |
straight |
T6853 |
19409-19418 |
NN |
denotes |
outgrowth |
T6854 |
19386-19389 |
CC |
denotes |
and |
T6855 |
19390-19400 |
JJ |
denotes |
unbranched |
T6856 |
19401-19408 |
NN |
denotes |
neurite |
T6857 |
19419-19420 |
-LRB- |
denotes |
( |
T6858 |
19427-19429 |
NN |
denotes |
4B |
T6859 |
19420-19426 |
NN |
denotes |
Figure |
T6860 |
19429-19430 |
-RRB- |
denotes |
) |
T6861 |
19430-19432 |
, |
denotes |
, |
T6862 |
19432-19437 |
IN |
denotes |
while |
T6863 |
19531-19539 |
VBD |
denotes |
resulted |
T6864 |
19438-19446 |
NNS |
denotes |
cultures |
T6865 |
19447-19452 |
VBN |
denotes |
grown |
T6866 |
19453-19455 |
IN |
denotes |
in |
T6867 |
19456-19459 |
DT |
denotes |
the |
T6868 |
19460-19468 |
NN |
denotes |
presence |
T6869 |
19469-19471 |
IN |
denotes |
of |
T6870 |
19472-19474 |
NN |
denotes |
NT |
T6871 |
19474-19475 |
HYPH |
denotes |
- |
T6872 |
19475-19476 |
CD |
denotes |
3 |
T6873 |
19476-19478 |
, |
denotes |
, |
T6874 |
19478-19483 |
WDT |
denotes |
which |
T6875 |
19484-19492 |
VBZ |
denotes |
supports |
T6876 |
19493-19501 |
NN |
denotes |
survival |
T6877 |
19502-19504 |
IN |
denotes |
of |
T6878 |
19505-19519 |
JJ |
denotes |
proprioceptive |
T6879 |
19520-19529 |
NNS |
denotes |
afferents |
T6880 |
19529-19531 |
, |
denotes |
, |
T6881 |
19540-19542 |
IN |
denotes |
in |
T6882 |
19543-19544 |
DT |
denotes |
a |
T6883 |
19579-19586 |
NN |
denotes |
pattern |
T6884 |
19545-19551 |
RB |
denotes |
highly |
T6885 |
19552-19560 |
VBN |
denotes |
branched |
T6886 |
19561-19568 |
NN |
denotes |
neurite |
T6887 |
19569-19578 |
NN |
denotes |
outgrowth |
T6888 |
19587-19592 |
IN |
denotes |
after |
T6889 |
19593-19595 |
CD |
denotes |
48 |
T6890 |
19596-19597 |
NN |
denotes |
h |
T6891 |
19598-19600 |
FW |
denotes |
in |
T6892 |
19601-19606 |
FW |
denotes |
vitro |
T6893 |
19607-19608 |
-LRB- |
denotes |
( |
T6894 |
19615-19617 |
NN |
denotes |
4C |
T6895 |
19608-19614 |
NN |
denotes |
Figure |
T6896 |
19617-19618 |
-RRB- |
denotes |
) |
T6897 |
19618-19619 |
. |
denotes |
. |
T6898 |
19619-19829 |
sentence |
denotes |
Surprisingly, DRG neurons isolated from TauEWS-Pea3/+ Isl1Cre/+ embryos and cultured without neurotrophic support survived after 48 h in vitro and had established long and highly branched neurites (Figure 4D). |
T6899 |
19620-19632 |
RB |
denotes |
Surprisingly |
T6900 |
19734-19742 |
VBD |
denotes |
survived |
T6901 |
19632-19634 |
, |
denotes |
, |
T6902 |
19634-19637 |
NN |
denotes |
DRG |
T6903 |
19638-19645 |
NNS |
denotes |
neurons |
T6904 |
19646-19654 |
VBN |
denotes |
isolated |
T6905 |
19655-19659 |
IN |
denotes |
from |
T6906 |
19660-19666 |
NN |
denotes |
TauEWS |
T6907 |
19674-19681 |
NN |
denotes |
Isl1Cre |
T6908 |
19666-19667 |
HYPH |
denotes |
- |
T6909 |
19667-19671 |
NN |
denotes |
Pea3 |
T6910 |
19671-19672 |
HYPH |
denotes |
/ |
T6911 |
19672-19673 |
SYM |
denotes |
+ |
T6912 |
19684-19691 |
NNS |
denotes |
embryos |
T6913 |
19681-19682 |
HYPH |
denotes |
/ |
T6914 |
19682-19683 |
SYM |
denotes |
+ |
T6915 |
19692-19695 |
CC |
denotes |
and |
T6916 |
19696-19704 |
VBN |
denotes |
cultured |
T6917 |
19705-19712 |
IN |
denotes |
without |
T6918 |
19713-19725 |
JJ |
denotes |
neurotrophic |
T6919 |
19726-19733 |
NN |
denotes |
support |
T6920 |
19743-19748 |
IN |
denotes |
after |
T6921 |
19749-19751 |
CD |
denotes |
48 |
T6922 |
19752-19753 |
NN |
denotes |
h |
T6923 |
19754-19756 |
FW |
denotes |
in |
T6924 |
19757-19762 |
FW |
denotes |
vitro |
T6925 |
19763-19766 |
CC |
denotes |
and |
T6926 |
19767-19770 |
VBD |
denotes |
had |
T6927 |
19771-19782 |
VBN |
denotes |
established |
T6928 |
19783-19787 |
JJ |
denotes |
long |
T6929 |
19808-19816 |
NNS |
denotes |
neurites |
T6930 |
19788-19791 |
CC |
denotes |
and |
T6931 |
19792-19798 |
RB |
denotes |
highly |
T6932 |
19799-19807 |
VBN |
denotes |
branched |
T6933 |
19817-19818 |
-LRB- |
denotes |
( |
T6934 |
19825-19827 |
NN |
denotes |
4D |
T6935 |
19818-19824 |
NN |
denotes |
Figure |
T6936 |
19827-19828 |
-RRB- |
denotes |
) |
T6937 |
19828-19829 |
. |
denotes |
. |
T6938 |
19829-19974 |
sentence |
denotes |
Neither the pattern of neurite outgrowth nor neuronal survival changed significantly after application of either NGF or NT-3 (Figure 4E and 4F). |
T6939 |
19830-19837 |
CC |
denotes |
Neither |
T6940 |
19842-19849 |
NN |
denotes |
pattern |
T6941 |
19838-19841 |
DT |
denotes |
the |
T6942 |
19893-19900 |
VBD |
denotes |
changed |
T6943 |
19850-19852 |
IN |
denotes |
of |
T6944 |
19853-19860 |
NN |
denotes |
neurite |
T6945 |
19861-19870 |
NN |
denotes |
outgrowth |
T6946 |
19871-19874 |
CC |
denotes |
nor |
T6947 |
19875-19883 |
JJ |
denotes |
neuronal |
T6948 |
19884-19892 |
NN |
denotes |
survival |
T6949 |
19901-19914 |
RB |
denotes |
significantly |
T6950 |
19915-19920 |
IN |
denotes |
after |
T6951 |
19921-19932 |
NN |
denotes |
application |
T6952 |
19933-19935 |
IN |
denotes |
of |
T6953 |
19936-19942 |
CC |
denotes |
either |
T6954 |
19943-19946 |
NN |
denotes |
NGF |
T6955 |
19947-19949 |
CC |
denotes |
or |
T6956 |
19950-19952 |
NN |
denotes |
NT |
T6957 |
19952-19953 |
HYPH |
denotes |
- |
T6958 |
19953-19954 |
CD |
denotes |
3 |
T6959 |
19955-19956 |
-LRB- |
denotes |
( |
T6960 |
19963-19965 |
NN |
denotes |
4E |
T6961 |
19956-19962 |
NN |
denotes |
Figure |
T6962 |
19966-19969 |
CC |
denotes |
and |
T6963 |
19970-19972 |
NN |
denotes |
4F |
T6964 |
19972-19973 |
-RRB- |
denotes |
) |
T6965 |
19973-19974 |
. |
denotes |
. |
T6966 |
19974-20667 |
sentence |
denotes |
Figure 4 Neurotrophin-Independent Neurite Outgrowth In Vitro of DRG Neurons Expressing EWS-Pea3 Precociously
E13.5 lumbar DRG from wild-type (A, B, and C), TauEWS-Pea3/+ Isl1Cre/+ (D, E, and F), or Bax−/− (G, H, and I) embryos cultured for 48 h without neurotrophic support (A, D, and G) or in the presence of NGF (B, E, and H) or NT-3 (C, F, and I) were stained for expression of neurofilament to visualize axonal extensions.
Scale bar: 130 μm. To directly compare neurotrophin dependence of DRG neurons expressing EWS-Pea3 from the Tau locus at a precocious versus isochronic time of onset, we generated a strain of mice in which Cre recombinase is expressed from the PV locus (Figure S4). |
T6967 |
20422-20424 |
TO |
denotes |
To |
T6968 |
20434-20441 |
VB |
denotes |
compare |
T6969 |
20425-20433 |
RB |
denotes |
directly |
T6970 |
20572-20581 |
VBD |
denotes |
generated |
T6971 |
20442-20454 |
NN |
denotes |
neurotrophin |
T6972 |
20455-20465 |
NN |
denotes |
dependence |
T6973 |
20466-20468 |
IN |
denotes |
of |
T6974 |
20469-20472 |
NN |
denotes |
DRG |
T6975 |
20473-20480 |
NNS |
denotes |
neurons |
T6976 |
20481-20491 |
VBG |
denotes |
expressing |
T6977 |
20492-20495 |
NN |
denotes |
EWS |
T6978 |
20496-20500 |
NN |
denotes |
Pea3 |
T6979 |
20495-20496 |
HYPH |
denotes |
- |
T6980 |
20501-20505 |
IN |
denotes |
from |
T6981 |
20506-20509 |
DT |
denotes |
the |
T6982 |
20514-20519 |
NN |
denotes |
locus |
T6983 |
20510-20513 |
NN |
denotes |
Tau |
T6984 |
20520-20522 |
IN |
denotes |
at |
T6985 |
20523-20524 |
DT |
denotes |
a |
T6986 |
20554-20558 |
NN |
denotes |
time |
T6987 |
20525-20535 |
JJ |
denotes |
precocious |
T6988 |
20536-20542 |
CC |
denotes |
versus |
T6989 |
20543-20553 |
JJ |
denotes |
isochronic |
T6990 |
20559-20561 |
IN |
denotes |
of |
T6991 |
20562-20567 |
NN |
denotes |
onset |
T6992 |
20567-20569 |
, |
denotes |
, |
T6993 |
20569-20571 |
PRP |
denotes |
we |
T6994 |
20582-20583 |
DT |
denotes |
a |
T6995 |
20584-20590 |
NN |
denotes |
strain |
T6996 |
20591-20593 |
IN |
denotes |
of |
T6997 |
20594-20598 |
NNS |
denotes |
mice |
T6998 |
20599-20601 |
IN |
denotes |
in |
T6999 |
20627-20636 |
VBN |
denotes |
expressed |
T7000 |
20602-20607 |
WDT |
denotes |
which |
T7001 |
20608-20611 |
NN |
denotes |
Cre |
T7002 |
20612-20623 |
NN |
denotes |
recombinase |
T7003 |
20624-20626 |
VBZ |
denotes |
is |
T7004 |
20637-20641 |
IN |
denotes |
from |
T7005 |
20642-20645 |
DT |
denotes |
the |
T7006 |
20649-20654 |
NN |
denotes |
locus |
T7007 |
20646-20648 |
NN |
denotes |
PV |
T7008 |
20655-20656 |
-LRB- |
denotes |
( |
T7009 |
20663-20665 |
NN |
denotes |
S4 |
T7010 |
20656-20662 |
NN |
denotes |
Figure |
T7011 |
20665-20666 |
-RRB- |
denotes |
) |
T7012 |
20666-20667 |
. |
denotes |
. |
T7013 |
20667-20852 |
sentence |
denotes |
The expression of GFP in TaumGFP/+ PVCre/+ was restricted to PV+ proprioceptive DRG neurons and mirrored the onset of expression of PV at approximately E14 (Figure S4; data not shown). |
T7014 |
20668-20671 |
DT |
denotes |
The |
T7015 |
20672-20682 |
NN |
denotes |
expression |
T7016 |
20715-20725 |
VBN |
denotes |
restricted |
T7017 |
20683-20685 |
IN |
denotes |
of |
T7018 |
20686-20689 |
NN |
denotes |
GFP |
T7019 |
20690-20692 |
IN |
denotes |
in |
T7020 |
20693-20700 |
NN |
denotes |
TaumGFP |
T7021 |
20703-20708 |
NN |
denotes |
PVCre |
T7022 |
20700-20701 |
HYPH |
denotes |
/ |
T7023 |
20701-20702 |
SYM |
denotes |
+ |
T7024 |
20708-20709 |
HYPH |
denotes |
/ |
T7025 |
20709-20710 |
SYM |
denotes |
+ |
T7026 |
20711-20714 |
VBD |
denotes |
was |
T7027 |
20726-20728 |
IN |
denotes |
to |
T7028 |
20729-20731 |
NN |
denotes |
PV |
T7029 |
20752-20759 |
NNS |
denotes |
neurons |
T7030 |
20731-20732 |
SYM |
denotes |
+ |
T7031 |
20733-20747 |
JJ |
denotes |
proprioceptive |
T7032 |
20748-20751 |
NN |
denotes |
DRG |
T7033 |
20760-20763 |
CC |
denotes |
and |
T7034 |
20764-20772 |
VBD |
denotes |
mirrored |
T7035 |
20773-20776 |
DT |
denotes |
the |
T7036 |
20777-20782 |
NN |
denotes |
onset |
T7037 |
20783-20785 |
IN |
denotes |
of |
T7038 |
20786-20796 |
NN |
denotes |
expression |
T7039 |
20797-20799 |
IN |
denotes |
of |
T7040 |
20800-20802 |
NN |
denotes |
PV |
T7041 |
20803-20805 |
IN |
denotes |
at |
T7042 |
20806-20819 |
RB |
denotes |
approximately |
T7043 |
20820-20823 |
NN |
denotes |
E14 |
T7044 |
20824-20825 |
-LRB- |
denotes |
( |
T7045 |
20832-20834 |
NN |
denotes |
S4 |
T7046 |
20825-20831 |
NN |
denotes |
Figure |
T7047 |
20834-20835 |
: |
denotes |
; |
T7048 |
20836-20840 |
NNS |
denotes |
data |
T7049 |
20845-20850 |
VBN |
denotes |
shown |
T7050 |
20841-20844 |
RB |
denotes |
not |
T7051 |
20850-20851 |
-RRB- |
denotes |
) |
T7052 |
20851-20852 |
. |
denotes |
. |
T7053 |
20852-21013 |
sentence |
denotes |
We next cultured E14.5 whole DRG explants from TauEWS-Pea3/+ PVCre/+ and TaumGFP/+ PVCre/+ mice for 48 h in vitro in the presence or absence of NT-3 (Figure 5). |
T7054 |
20853-20855 |
PRP |
denotes |
We |
T7055 |
20861-20869 |
VBN |
denotes |
cultured |
T7056 |
20856-20860 |
RB |
denotes |
next |
T7057 |
20870-20875 |
NN |
denotes |
E14.5 |
T7058 |
20886-20894 |
NNS |
denotes |
explants |
T7059 |
20876-20881 |
JJ |
denotes |
whole |
T7060 |
20882-20885 |
NN |
denotes |
DRG |
T7061 |
20895-20899 |
IN |
denotes |
from |
T7062 |
20900-20906 |
NN |
denotes |
TauEWS |
T7063 |
20914-20919 |
NN |
denotes |
PVCre |
T7064 |
20906-20907 |
HYPH |
denotes |
- |
T7065 |
20907-20911 |
NN |
denotes |
Pea3 |
T7066 |
20911-20912 |
HYPH |
denotes |
/ |
T7067 |
20912-20913 |
SYM |
denotes |
+ |
T7068 |
20944-20948 |
NNS |
denotes |
mice |
T7069 |
20919-20920 |
HYPH |
denotes |
/ |
T7070 |
20920-20921 |
SYM |
denotes |
+ |
T7071 |
20922-20925 |
CC |
denotes |
and |
T7072 |
20926-20933 |
NN |
denotes |
TaumGFP |
T7073 |
20936-20941 |
NN |
denotes |
PVCre |
T7074 |
20933-20934 |
HYPH |
denotes |
/ |
T7075 |
20934-20935 |
SYM |
denotes |
+ |
T7076 |
20941-20942 |
HYPH |
denotes |
/ |
T7077 |
20942-20943 |
SYM |
denotes |
+ |
T7078 |
20949-20952 |
IN |
denotes |
for |
T7079 |
20953-20955 |
CD |
denotes |
48 |
T7080 |
20956-20957 |
NN |
denotes |
h |
T7081 |
20958-20960 |
FW |
denotes |
in |
T7082 |
20961-20966 |
FW |
denotes |
vitro |
T7083 |
20967-20969 |
IN |
denotes |
in |
T7084 |
20970-20973 |
DT |
denotes |
the |
T7085 |
20974-20982 |
NN |
denotes |
presence |
T7086 |
20983-20985 |
CC |
denotes |
or |
T7087 |
20986-20993 |
NN |
denotes |
absence |
T7088 |
20994-20996 |
IN |
denotes |
of |
T7089 |
20997-20999 |
NN |
denotes |
NT |
T7090 |
20999-21000 |
HYPH |
denotes |
- |
T7091 |
21000-21001 |
CD |
denotes |
3 |
T7092 |
21002-21003 |
-LRB- |
denotes |
( |
T7093 |
21003-21009 |
NN |
denotes |
Figure |
T7094 |
21010-21011 |
CD |
denotes |
5 |
T7095 |
21011-21012 |
-RRB- |
denotes |
) |
T7096 |
21012-21013 |
. |
denotes |
. |
T7097 |
21013-21173 |
sentence |
denotes |
We found that DRG neurons from both genotypes survived and extended neurites only in the presence of NT-3, whereas they died in the absence of NT-3 (Figure 5). |
T7098 |
21014-21016 |
PRP |
denotes |
We |
T7099 |
21017-21022 |
VBD |
denotes |
found |
T7100 |
21023-21027 |
IN |
denotes |
that |
T7101 |
21060-21068 |
VBD |
denotes |
survived |
T7102 |
21028-21031 |
NN |
denotes |
DRG |
T7103 |
21032-21039 |
NNS |
denotes |
neurons |
T7104 |
21040-21044 |
IN |
denotes |
from |
T7105 |
21045-21049 |
DT |
denotes |
both |
T7106 |
21050-21059 |
NNS |
denotes |
genotypes |
T7107 |
21069-21072 |
CC |
denotes |
and |
T7108 |
21073-21081 |
VBD |
denotes |
extended |
T7109 |
21082-21090 |
NNS |
denotes |
neurites |
T7110 |
21091-21095 |
RB |
denotes |
only |
T7111 |
21096-21098 |
IN |
denotes |
in |
T7112 |
21099-21102 |
DT |
denotes |
the |
T7113 |
21103-21111 |
NN |
denotes |
presence |
T7114 |
21112-21114 |
IN |
denotes |
of |
T7115 |
21115-21117 |
NN |
denotes |
NT |
T7116 |
21117-21118 |
HYPH |
denotes |
- |
T7117 |
21118-21119 |
CD |
denotes |
3 |
T7118 |
21119-21121 |
, |
denotes |
, |
T7119 |
21121-21128 |
IN |
denotes |
whereas |
T7120 |
21134-21138 |
VBD |
denotes |
died |
T7121 |
21129-21133 |
PRP |
denotes |
they |
T7122 |
21139-21141 |
IN |
denotes |
in |
T7123 |
21142-21145 |
DT |
denotes |
the |
T7124 |
21146-21153 |
NN |
denotes |
absence |
T7125 |
21154-21156 |
IN |
denotes |
of |
T7126 |
21157-21159 |
NN |
denotes |
NT |
T7127 |
21159-21160 |
HYPH |
denotes |
- |
T7128 |
21160-21161 |
CD |
denotes |
3 |
T7129 |
21162-21163 |
-LRB- |
denotes |
( |
T7130 |
21163-21169 |
NN |
denotes |
Figure |
T7131 |
21170-21171 |
CD |
denotes |
5 |
T7132 |
21171-21172 |
-RRB- |
denotes |
) |
T7133 |
21172-21173 |
. |
denotes |
. |
T7134 |
21173-21413 |
sentence |
denotes |
Together, these findings suggest that only precocious but not isochronic ETS signaling in DRG neurons is capable of uncoupling survival and neurite outgrowth from a requirement for neurotrophin signaling normally observed in wild-type DRG. |
T7135 |
21174-21182 |
RB |
denotes |
Together |
T7136 |
21199-21206 |
VBP |
denotes |
suggest |
T7137 |
21182-21184 |
, |
denotes |
, |
T7138 |
21184-21189 |
DT |
denotes |
these |
T7139 |
21190-21198 |
NNS |
denotes |
findings |
T7140 |
21207-21211 |
IN |
denotes |
that |
T7141 |
21276-21278 |
VBZ |
denotes |
is |
T7142 |
21212-21216 |
RB |
denotes |
only |
T7143 |
21251-21260 |
NN |
denotes |
signaling |
T7144 |
21217-21227 |
JJ |
denotes |
precocious |
T7145 |
21228-21231 |
CC |
denotes |
but |
T7146 |
21232-21235 |
RB |
denotes |
not |
T7147 |
21236-21246 |
JJ |
denotes |
isochronic |
T7148 |
21247-21250 |
NN |
denotes |
ETS |
T7149 |
21261-21263 |
IN |
denotes |
in |
T7150 |
21264-21267 |
NN |
denotes |
DRG |
T7151 |
21268-21275 |
NNS |
denotes |
neurons |
T7152 |
21279-21286 |
JJ |
denotes |
capable |
T7153 |
21287-21289 |
IN |
denotes |
of |
T7154 |
21290-21300 |
VBG |
denotes |
uncoupling |
T7155 |
21301-21309 |
NN |
denotes |
survival |
T7156 |
21310-21313 |
CC |
denotes |
and |
T7157 |
21314-21321 |
NN |
denotes |
neurite |
T7158 |
21322-21331 |
NN |
denotes |
outgrowth |
T7159 |
21332-21336 |
IN |
denotes |
from |
T7160 |
21337-21338 |
DT |
denotes |
a |
T7161 |
21339-21350 |
NN |
denotes |
requirement |
T7162 |
21351-21354 |
IN |
denotes |
for |
T7163 |
21355-21367 |
NN |
denotes |
neurotrophin |
T7164 |
21368-21377 |
NN |
denotes |
signaling |
T7165 |
21378-21386 |
RB |
denotes |
normally |
T7166 |
21387-21395 |
VBN |
denotes |
observed |
T7167 |
21396-21398 |
IN |
denotes |
in |
T7168 |
21399-21403 |
JJ |
denotes |
wild |
T7169 |
21404-21408 |
NN |
denotes |
type |
T7170 |
21403-21404 |
HYPH |
denotes |
- |
T7171 |
21409-21412 |
NN |
denotes |
DRG |
T7172 |
21412-21413 |
. |
denotes |
. |
T7173 |
21413-22134 |
sentence |
denotes |
Figure 5 DRG Neurons Expressing EWS-Pea3 Isochronically Depend on Neurotrophins for Survival
E14.5 lumbar DRG from TaumGFP/+ PVCre/+ (A and B) and TauEWS-Pea3/+ PVCre/+ (C and D) embryos cultured for 48 h without neurotrophic support (A and C) or in the presence of NT-3 (B and D) were stained for expression of neurofilament (red) and LacZ (green) to visualize axonal extensions and survival of PV-expressing proprioceptive afferents.
Scale bar: 150 μm. To determine whether neuronal survival of DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos in the absence of neurotrophic support is sufficient to explain the observed neuronal outgrowth, we analyzed DRG isolated from mice mutant in the proapoptotic gene Bax [27]. |
T7174 |
21870-21872 |
TO |
denotes |
To |
T7175 |
21873-21882 |
VB |
denotes |
determine |
T7176 |
22061-22069 |
VBD |
denotes |
analyzed |
T7177 |
21883-21890 |
IN |
denotes |
whether |
T7178 |
22000-22002 |
VBZ |
denotes |
is |
T7179 |
21891-21899 |
JJ |
denotes |
neuronal |
T7180 |
21900-21908 |
NN |
denotes |
survival |
T7181 |
21909-21911 |
IN |
denotes |
of |
T7182 |
21912-21915 |
NN |
denotes |
DRG |
T7183 |
21916-21923 |
NNS |
denotes |
neurons |
T7184 |
21924-21928 |
IN |
denotes |
from |
T7185 |
21929-21935 |
NN |
denotes |
TauEWS |
T7186 |
21943-21950 |
NN |
denotes |
Isl1Cre |
T7187 |
21935-21936 |
HYPH |
denotes |
- |
T7188 |
21936-21940 |
NN |
denotes |
Pea3 |
T7189 |
21940-21941 |
HYPH |
denotes |
/ |
T7190 |
21941-21942 |
SYM |
denotes |
+ |
T7191 |
21953-21960 |
NNS |
denotes |
embryos |
T7192 |
21950-21951 |
HYPH |
denotes |
/ |
T7193 |
21951-21952 |
SYM |
denotes |
+ |
T7194 |
21961-21963 |
IN |
denotes |
in |
T7195 |
21964-21967 |
DT |
denotes |
the |
T7196 |
21968-21975 |
NN |
denotes |
absence |
T7197 |
21976-21978 |
IN |
denotes |
of |
T7198 |
21979-21991 |
JJ |
denotes |
neurotrophic |
T7199 |
21992-21999 |
NN |
denotes |
support |
T7200 |
22003-22013 |
JJ |
denotes |
sufficient |
T7201 |
22014-22016 |
TO |
denotes |
to |
T7202 |
22017-22024 |
VB |
denotes |
explain |
T7203 |
22025-22028 |
DT |
denotes |
the |
T7204 |
22047-22056 |
NN |
denotes |
outgrowth |
T7205 |
22029-22037 |
VBN |
denotes |
observed |
T7206 |
22038-22046 |
JJ |
denotes |
neuronal |
T7207 |
22056-22058 |
, |
denotes |
, |
T7208 |
22058-22060 |
PRP |
denotes |
we |
T7209 |
22070-22073 |
NN |
denotes |
DRG |
T7210 |
22074-22082 |
VBN |
denotes |
isolated |
T7211 |
22083-22087 |
IN |
denotes |
from |
T7212 |
22088-22092 |
NNS |
denotes |
mice |
T7213 |
22093-22099 |
NN |
denotes |
mutant |
T7214 |
22100-22102 |
IN |
denotes |
in |
T7215 |
22103-22106 |
DT |
denotes |
the |
T7216 |
22125-22128 |
NN |
denotes |
Bax |
T7217 |
22107-22119 |
JJ |
denotes |
proapoptotic |
T7218 |
22120-22124 |
NN |
denotes |
gene |
T7219 |
22129-22130 |
-LRB- |
denotes |
[ |
T7220 |
22130-22132 |
CD |
denotes |
27 |
T7221 |
22132-22133 |
-RRB- |
denotes |
] |
T7222 |
22133-22134 |
. |
denotes |
. |
T7223 |
22134-22231 |
sentence |
denotes |
Consistent with previous results, Bax−/− DRG neurons survived without neurotrophic support [28]. |
T7224 |
22135-22145 |
JJ |
denotes |
Consistent |
T7225 |
22188-22196 |
VBD |
denotes |
survived |
T7226 |
22146-22150 |
IN |
denotes |
with |
T7227 |
22151-22159 |
JJ |
denotes |
previous |
T7228 |
22160-22167 |
NNS |
denotes |
results |
T7229 |
22167-22169 |
, |
denotes |
, |
T7230 |
22169-22172 |
NN |
denotes |
Bax |
T7231 |
22180-22187 |
NNS |
denotes |
neurons |
T7232 |
22172-22173 |
SYM |
denotes |
− |
T7233 |
22173-22174 |
HYPH |
denotes |
/ |
T7234 |
22174-22175 |
SYM |
denotes |
− |
T7235 |
22176-22179 |
NN |
denotes |
DRG |
T7236 |
22197-22204 |
IN |
denotes |
without |
T7237 |
22205-22217 |
JJ |
denotes |
neurotrophic |
T7238 |
22218-22225 |
NN |
denotes |
support |
T7239 |
22226-22227 |
-LRB- |
denotes |
[ |
T7240 |
22227-22229 |
CD |
denotes |
28 |
T7241 |
22229-22230 |
-RRB- |
denotes |
] |
T7242 |
22230-22231 |
. |
denotes |
. |
T7243 |
22231-22543 |
sentence |
denotes |
In contrast, neurite outgrowth of Bax−/− DRG neurons was significantly less (see Figure 4G) than that of either DRG from TauEWS-Pea3/+ Isl1Cre/+ embryos cultured in the absence of neurotrophic support (see Figure 4D) or Bax−/− DRG neurons cultured in the presence of neurotrophic support (see Figure 4H and 4I). |
T7244 |
22232-22234 |
IN |
denotes |
In |
T7245 |
22285-22288 |
VBD |
denotes |
was |
T7246 |
22235-22243 |
NN |
denotes |
contrast |
T7247 |
22243-22245 |
, |
denotes |
, |
T7248 |
22245-22252 |
NN |
denotes |
neurite |
T7249 |
22253-22262 |
NN |
denotes |
outgrowth |
T7250 |
22263-22265 |
IN |
denotes |
of |
T7251 |
22266-22269 |
NN |
denotes |
Bax |
T7252 |
22277-22284 |
NNS |
denotes |
neurons |
T7253 |
22269-22270 |
SYM |
denotes |
− |
T7254 |
22270-22271 |
HYPH |
denotes |
/ |
T7255 |
22271-22272 |
SYM |
denotes |
− |
T7256 |
22273-22276 |
NN |
denotes |
DRG |
T7257 |
22289-22302 |
RB |
denotes |
significantly |
T7258 |
22303-22307 |
JJR |
denotes |
less |
T7259 |
22308-22309 |
-LRB- |
denotes |
( |
T7260 |
22309-22312 |
VB |
denotes |
see |
T7261 |
22313-22319 |
NN |
denotes |
Figure |
T7262 |
22320-22322 |
NN |
denotes |
4G |
T7263 |
22322-22323 |
-RRB- |
denotes |
) |
T7264 |
22324-22328 |
IN |
denotes |
than |
T7265 |
22329-22333 |
DT |
denotes |
that |
T7266 |
22334-22336 |
IN |
denotes |
of |
T7267 |
22337-22343 |
CC |
denotes |
either |
T7268 |
22344-22347 |
NN |
denotes |
DRG |
T7269 |
22348-22352 |
IN |
denotes |
from |
T7270 |
22353-22359 |
NN |
denotes |
TauEWS |
T7271 |
22367-22374 |
NN |
denotes |
Isl1Cre |
T7272 |
22359-22360 |
HYPH |
denotes |
- |
T7273 |
22360-22364 |
NN |
denotes |
Pea3 |
T7274 |
22364-22365 |
HYPH |
denotes |
/ |
T7275 |
22365-22366 |
SYM |
denotes |
+ |
T7276 |
22377-22384 |
NNS |
denotes |
embryos |
T7277 |
22374-22375 |
HYPH |
denotes |
/ |
T7278 |
22375-22376 |
SYM |
denotes |
+ |
T7279 |
22385-22393 |
VBN |
denotes |
cultured |
T7280 |
22394-22396 |
IN |
denotes |
in |
T7281 |
22397-22400 |
DT |
denotes |
the |
T7282 |
22401-22408 |
NN |
denotes |
absence |
T7283 |
22409-22411 |
IN |
denotes |
of |
T7284 |
22412-22424 |
JJ |
denotes |
neurotrophic |
T7285 |
22425-22432 |
NN |
denotes |
support |
T7286 |
22433-22434 |
-LRB- |
denotes |
( |
T7287 |
22434-22437 |
VB |
denotes |
see |
T7288 |
22438-22444 |
NN |
denotes |
Figure |
T7289 |
22445-22447 |
NN |
denotes |
4D |
T7290 |
22447-22448 |
-RRB- |
denotes |
) |
T7291 |
22449-22451 |
CC |
denotes |
or |
T7292 |
22452-22455 |
NN |
denotes |
Bax |
T7293 |
22463-22470 |
NNS |
denotes |
neurons |
T7294 |
22455-22456 |
SYM |
denotes |
− |
T7295 |
22456-22457 |
HYPH |
denotes |
/ |
T7296 |
22457-22458 |
SYM |
denotes |
− |
T7297 |
22459-22462 |
NN |
denotes |
DRG |
T7298 |
22471-22479 |
VBN |
denotes |
cultured |
T7299 |
22480-22482 |
IN |
denotes |
in |
T7300 |
22483-22486 |
DT |
denotes |
the |
T7301 |
22487-22495 |
NN |
denotes |
presence |
T7302 |
22496-22498 |
IN |
denotes |
of |
T7303 |
22499-22511 |
JJ |
denotes |
neurotrophic |
T7304 |
22512-22519 |
NN |
denotes |
support |
T7305 |
22520-22521 |
-LRB- |
denotes |
( |
T7306 |
22521-22524 |
VB |
denotes |
see |
T7307 |
22525-22531 |
NN |
denotes |
Figure |
T7308 |
22532-22534 |
NN |
denotes |
4H |
T7309 |
22535-22538 |
CC |
denotes |
and |
T7310 |
22539-22541 |
NN |
denotes |
4I |
T7311 |
22541-22542 |
-RRB- |
denotes |
) |
T7312 |
22542-22543 |
. |
denotes |
. |
T7313 |
22543-22763 |
sentence |
denotes |
These findings suggest that in addition to mediating neurotrophin-independent neuronal survival, expression of EWS-Pea3 in early post-mitotic neurons also promotes neurite outgrowth in a neurotrophin-independent manner. |
T7314 |
22544-22549 |
DT |
denotes |
These |
T7315 |
22550-22558 |
NNS |
denotes |
findings |
T7316 |
22559-22566 |
VBP |
denotes |
suggest |
T7317 |
22567-22571 |
IN |
denotes |
that |
T7318 |
22699-22707 |
VBZ |
denotes |
promotes |
T7319 |
22572-22574 |
IN |
denotes |
in |
T7320 |
22575-22583 |
NN |
denotes |
addition |
T7321 |
22584-22586 |
IN |
denotes |
to |
T7322 |
22587-22596 |
VBG |
denotes |
mediating |
T7323 |
22597-22609 |
NN |
denotes |
neurotrophin |
T7324 |
22610-22621 |
JJ |
denotes |
independent |
T7325 |
22609-22610 |
HYPH |
denotes |
- |
T7326 |
22631-22639 |
NN |
denotes |
survival |
T7327 |
22622-22630 |
JJ |
denotes |
neuronal |
T7328 |
22639-22641 |
, |
denotes |
, |
T7329 |
22641-22651 |
NN |
denotes |
expression |
T7330 |
22652-22654 |
IN |
denotes |
of |
T7331 |
22655-22658 |
NN |
denotes |
EWS |
T7332 |
22659-22663 |
NN |
denotes |
Pea3 |
T7333 |
22658-22659 |
HYPH |
denotes |
- |
T7334 |
22664-22666 |
IN |
denotes |
in |
T7335 |
22667-22672 |
JJ |
denotes |
early |
T7336 |
22686-22693 |
NNS |
denotes |
neurons |
T7337 |
22673-22685 |
JJ |
denotes |
post-mitotic |
T7338 |
22694-22698 |
RB |
denotes |
also |
T7339 |
22708-22715 |
NN |
denotes |
neurite |
T7340 |
22716-22725 |
NN |
denotes |
outgrowth |
T7341 |
22726-22728 |
IN |
denotes |
in |
T7342 |
22729-22730 |
DT |
denotes |
a |
T7343 |
22756-22762 |
NN |
denotes |
manner |
T7344 |
22731-22743 |
NN |
denotes |
neurotrophin |
T7345 |
22744-22755 |
JJ |
denotes |
independent |
T7346 |
22743-22744 |
HYPH |
denotes |
- |
T7347 |
22762-22763 |
. |
denotes |
. |
T7348 |
22763-23036 |
sentence |
denotes |
To begin to assess at which step of the neurotrophin signaling cascade DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos become unresponsive to the addition of neurotrophins, we assayed the expression of neurotrophin receptors in TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure 6). |
T7349 |
22764-22766 |
TO |
denotes |
To |
T7350 |
22767-22772 |
VB |
denotes |
begin |
T7351 |
22941-22948 |
VBD |
denotes |
assayed |
T7352 |
22773-22775 |
TO |
denotes |
to |
T7353 |
22776-22782 |
VB |
denotes |
assess |
T7354 |
22783-22785 |
IN |
denotes |
at |
T7355 |
22884-22890 |
VBP |
denotes |
become |
T7356 |
22786-22791 |
WDT |
denotes |
which |
T7357 |
22792-22796 |
NN |
denotes |
step |
T7358 |
22797-22799 |
IN |
denotes |
of |
T7359 |
22800-22803 |
DT |
denotes |
the |
T7360 |
22827-22834 |
NN |
denotes |
cascade |
T7361 |
22804-22816 |
NN |
denotes |
neurotrophin |
T7362 |
22817-22826 |
NN |
denotes |
signaling |
T7363 |
22835-22838 |
NN |
denotes |
DRG |
T7364 |
22839-22846 |
NNS |
denotes |
neurons |
T7365 |
22847-22851 |
IN |
denotes |
from |
T7366 |
22852-22858 |
NN |
denotes |
TauEWS |
T7367 |
22866-22873 |
NN |
denotes |
Isl1Cre |
T7368 |
22858-22859 |
HYPH |
denotes |
- |
T7369 |
22859-22863 |
NN |
denotes |
Pea3 |
T7370 |
22863-22864 |
HYPH |
denotes |
/ |
T7371 |
22864-22865 |
SYM |
denotes |
+ |
T7372 |
22876-22883 |
NNS |
denotes |
embryos |
T7373 |
22873-22874 |
HYPH |
denotes |
/ |
T7374 |
22874-22875 |
SYM |
denotes |
+ |
T7375 |
22891-22903 |
JJ |
denotes |
unresponsive |
T7376 |
22904-22906 |
IN |
denotes |
to |
T7377 |
22907-22910 |
DT |
denotes |
the |
T7378 |
22911-22919 |
NN |
denotes |
addition |
T7379 |
22920-22922 |
IN |
denotes |
of |
T7380 |
22923-22936 |
NNS |
denotes |
neurotrophins |
T7381 |
22936-22938 |
, |
denotes |
, |
T7382 |
22938-22940 |
PRP |
denotes |
we |
T7383 |
22949-22952 |
DT |
denotes |
the |
T7384 |
22953-22963 |
NN |
denotes |
expression |
T7385 |
22964-22966 |
IN |
denotes |
of |
T7386 |
22967-22979 |
NN |
denotes |
neurotrophin |
T7387 |
22980-22989 |
NNS |
denotes |
receptors |
T7388 |
22990-22992 |
IN |
denotes |
in |
T7389 |
22993-22999 |
NN |
denotes |
TauEWS |
T7390 |
23007-23014 |
NN |
denotes |
Isl1Cre |
T7391 |
22999-23000 |
HYPH |
denotes |
- |
T7392 |
23000-23004 |
NN |
denotes |
Pea3 |
T7393 |
23004-23005 |
HYPH |
denotes |
/ |
T7394 |
23005-23006 |
SYM |
denotes |
+ |
T7395 |
23017-23024 |
NNS |
denotes |
embryos |
T7396 |
23014-23015 |
HYPH |
denotes |
/ |
T7397 |
23015-23016 |
SYM |
denotes |
+ |
T7398 |
23025-23026 |
-LRB- |
denotes |
( |
T7399 |
23026-23032 |
NN |
denotes |
Figure |
T7400 |
23033-23034 |
CD |
denotes |
6 |
T7401 |
23034-23035 |
-RRB- |
denotes |
) |
T7402 |
23035-23036 |
. |
denotes |
. |
T7403 |
23036-23338 |
sentence |
denotes |
Whereas expression of the neurotrophin receptors TrkA, TrkB, and TrkC marks afferents of distinct sensory modalities in DRG of wild-type embryos (Figure 6A–6C) [4,29], TauEWS-Pea3/+ Isl1Cre/+ embryos showed complete absence of expression of TrkA, TrkB, and TrkC in DRG neurons at E16.5 (Figure 6G–6I). |
T7404 |
23037-23044 |
IN |
denotes |
Whereas |
T7405 |
23107-23112 |
VBZ |
denotes |
marks |
T7406 |
23045-23055 |
NN |
denotes |
expression |
T7407 |
23056-23058 |
IN |
denotes |
of |
T7408 |
23059-23062 |
DT |
denotes |
the |
T7409 |
23076-23085 |
NNS |
denotes |
receptors |
T7410 |
23063-23075 |
NN |
denotes |
neurotrophin |
T7411 |
23086-23090 |
NN |
denotes |
TrkA |
T7412 |
23090-23092 |
, |
denotes |
, |
T7413 |
23092-23096 |
NN |
denotes |
TrkB |
T7414 |
23096-23098 |
, |
denotes |
, |
T7415 |
23098-23101 |
CC |
denotes |
and |
T7416 |
23102-23106 |
NN |
denotes |
TrkC |
T7417 |
23237-23243 |
VBD |
denotes |
showed |
T7418 |
23113-23122 |
NNS |
denotes |
afferents |
T7419 |
23123-23125 |
IN |
denotes |
of |
T7420 |
23126-23134 |
JJ |
denotes |
distinct |
T7421 |
23143-23153 |
NNS |
denotes |
modalities |
T7422 |
23135-23142 |
JJ |
denotes |
sensory |
T7423 |
23154-23156 |
IN |
denotes |
in |
T7424 |
23157-23160 |
NN |
denotes |
DRG |
T7425 |
23161-23163 |
IN |
denotes |
of |
T7426 |
23164-23168 |
JJ |
denotes |
wild |
T7427 |
23169-23173 |
NN |
denotes |
type |
T7428 |
23168-23169 |
HYPH |
denotes |
- |
T7429 |
23174-23181 |
NNS |
denotes |
embryos |
T7430 |
23182-23183 |
-LRB- |
denotes |
( |
T7431 |
23190-23192 |
NN |
denotes |
6A |
T7432 |
23183-23189 |
NN |
denotes |
Figure |
T7433 |
23192-23193 |
SYM |
denotes |
– |
T7434 |
23193-23195 |
NN |
denotes |
6C |
T7435 |
23195-23196 |
-RRB- |
denotes |
) |
T7436 |
23197-23198 |
-LRB- |
denotes |
[ |
T7437 |
23200-23202 |
CD |
denotes |
29 |
T7438 |
23198-23199 |
CD |
denotes |
4 |
T7439 |
23199-23200 |
, |
denotes |
, |
T7440 |
23202-23203 |
-RRB- |
denotes |
] |
T7441 |
23203-23205 |
, |
denotes |
, |
T7442 |
23205-23211 |
NN |
denotes |
TauEWS |
T7443 |
23219-23226 |
NN |
denotes |
Isl1Cre |
T7444 |
23211-23212 |
HYPH |
denotes |
- |
T7445 |
23212-23216 |
NN |
denotes |
Pea3 |
T7446 |
23216-23217 |
HYPH |
denotes |
/ |
T7447 |
23217-23218 |
SYM |
denotes |
+ |
T7448 |
23229-23236 |
NNS |
denotes |
embryos |
T7449 |
23226-23227 |
HYPH |
denotes |
/ |
T7450 |
23227-23228 |
SYM |
denotes |
+ |
T7451 |
23244-23252 |
JJ |
denotes |
complete |
T7452 |
23253-23260 |
NN |
denotes |
absence |
T7453 |
23261-23263 |
IN |
denotes |
of |
T7454 |
23264-23274 |
NN |
denotes |
expression |
T7455 |
23275-23277 |
IN |
denotes |
of |
T7456 |
23278-23282 |
NN |
denotes |
TrkA |
T7457 |
23282-23284 |
, |
denotes |
, |
T7458 |
23284-23288 |
NN |
denotes |
TrkB |
T7459 |
23288-23290 |
, |
denotes |
, |
T7460 |
23290-23293 |
CC |
denotes |
and |
T7461 |
23294-23298 |
NN |
denotes |
TrkC |
T7462 |
23299-23301 |
IN |
denotes |
in |
T7463 |
23302-23305 |
NN |
denotes |
DRG |
T7464 |
23306-23313 |
NNS |
denotes |
neurons |
T7465 |
23314-23316 |
IN |
denotes |
at |
T7466 |
23317-23322 |
NN |
denotes |
E16.5 |
T7467 |
23323-23324 |
-LRB- |
denotes |
( |
T7468 |
23331-23333 |
NN |
denotes |
6G |
T7469 |
23324-23330 |
NN |
denotes |
Figure |
T7470 |
23333-23334 |
SYM |
denotes |
– |
T7471 |
23334-23336 |
NN |
denotes |
6I |
T7472 |
23336-23337 |
-RRB- |
denotes |
) |
T7473 |
23337-23338 |
. |
denotes |
. |
T7474 |
23338-23539 |
sentence |
denotes |
This absence of Trk receptor expression in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos provides a likely explanation for the lack of responsiveness of these neurons to the addition of neurotrophic factors. |
T7475 |
23339-23343 |
DT |
denotes |
This |
T7476 |
23344-23351 |
NN |
denotes |
absence |
T7477 |
23421-23429 |
VBZ |
denotes |
provides |
T7478 |
23352-23354 |
IN |
denotes |
of |
T7479 |
23355-23358 |
NN |
denotes |
Trk |
T7480 |
23368-23378 |
NN |
denotes |
expression |
T7481 |
23359-23367 |
NN |
denotes |
receptor |
T7482 |
23379-23381 |
IN |
denotes |
in |
T7483 |
23382-23385 |
NN |
denotes |
DRG |
T7484 |
23386-23388 |
IN |
denotes |
of |
T7485 |
23389-23395 |
NN |
denotes |
TauEWS |
T7486 |
23403-23410 |
NN |
denotes |
Isl1Cre |
T7487 |
23395-23396 |
HYPH |
denotes |
- |
T7488 |
23396-23400 |
NN |
denotes |
Pea3 |
T7489 |
23400-23401 |
HYPH |
denotes |
/ |
T7490 |
23401-23402 |
SYM |
denotes |
+ |
T7491 |
23413-23420 |
NNS |
denotes |
embryos |
T7492 |
23410-23411 |
HYPH |
denotes |
/ |
T7493 |
23411-23412 |
SYM |
denotes |
+ |
T7494 |
23430-23431 |
DT |
denotes |
a |
T7495 |
23439-23450 |
NN |
denotes |
explanation |
T7496 |
23432-23438 |
JJ |
denotes |
likely |
T7497 |
23451-23454 |
IN |
denotes |
for |
T7498 |
23455-23458 |
DT |
denotes |
the |
T7499 |
23459-23463 |
NN |
denotes |
lack |
T7500 |
23464-23466 |
IN |
denotes |
of |
T7501 |
23467-23481 |
NN |
denotes |
responsiveness |
T7502 |
23482-23484 |
IN |
denotes |
of |
T7503 |
23485-23490 |
DT |
denotes |
these |
T7504 |
23491-23498 |
NNS |
denotes |
neurons |
T7505 |
23499-23501 |
IN |
denotes |
to |
T7506 |
23502-23505 |
DT |
denotes |
the |
T7507 |
23506-23514 |
NN |
denotes |
addition |
T7508 |
23515-23517 |
IN |
denotes |
of |
T7509 |
23518-23530 |
JJ |
denotes |
neurotrophic |
T7510 |
23531-23538 |
NNS |
denotes |
factors |
T7511 |
23538-23539 |
. |
denotes |
. |
T7512 |
23539-25144 |
sentence |
denotes |
Figure 6 Loss of Trk Receptor Expression and Increased Survival in DRG Neurons upon Precocious ETS Signaling
(A–C and G–I) In situ hybridization analysis of TrkA (A and G), TrkB (B and H), and TrkC (C and I) expression in E16.5 lumbar DRG of wild-type (A–C) and TauEWS-Pea3/+ Isl1Cre/+ (G–I) embryos.
(D–F and J–L) Analysis of lumbar DRG of wild-type (D), TaumGFP/+ IslCre/+ (E and F), and TauEWS-Pea3/+ Isl1Cre/+ (J, K, and L) embryos for (1) neuronal cell death at E13.5 by TUNEL (green; D and J), (2) cell survival and proliferation at E16.5 by LacZ (blue) wholemount staining (E and K; lumbar levels L1 and L2 are shown), and (3) BrdU (green)/LacZ (red) double labeling (F and L).
(M and N) Quantitative analysis (n ≥ 3 independent experiments) of the mean number of apoptotic events relative to wild-type levels is shown in (M) and neuronal survival in (N) as percent of wild-type of DRG at lumbar levels L1 to L5 as quantified on serial sections.
(O) Western blot analysis of protein extracts isolated from lumbar DRG of E16.5 wild-type (wt) and TauEWS-Pea3/+ Isl1Cre/+ (mut) embryos using the following antibodies: Akt, p-Akt (Ser473), CREB, p-CREB (Ser133), Bax, Bcl2, and Bcl-xl.
(P) Quantitative analysis of protein levels relative to wild-type in percent is shown on the right (n = 3 independent experiments).
Scale bar: (A–C and G–I), 35 μm; (D and J), 40 μm; (E and K), 200 μm; (F and L), 50 μm. We next assayed whether the complete absence of Trk receptor expression in TauEWS-Pea3/+ Isl1Cre/+ embryos had an influence on naturally occurring cell death in vivo using TUNEL on DRG sections. |
T7513 |
24950-24952 |
PRP |
denotes |
We |
T7514 |
24958-24965 |
VBD |
denotes |
assayed |
T7515 |
24953-24957 |
RB |
denotes |
next |
T7516 |
24966-24973 |
IN |
denotes |
whether |
T7517 |
25057-25060 |
VBD |
denotes |
had |
T7518 |
24974-24977 |
DT |
denotes |
the |
T7519 |
24987-24994 |
NN |
denotes |
absence |
T7520 |
24978-24986 |
JJ |
denotes |
complete |
T7521 |
24995-24997 |
IN |
denotes |
of |
T7522 |
24998-25001 |
NN |
denotes |
Trk |
T7523 |
25011-25021 |
NN |
denotes |
expression |
T7524 |
25002-25010 |
NN |
denotes |
receptor |
T7525 |
25022-25024 |
IN |
denotes |
in |
T7526 |
25025-25031 |
NN |
denotes |
TauEWS |
T7527 |
25039-25046 |
NN |
denotes |
Isl1Cre |
T7528 |
25031-25032 |
HYPH |
denotes |
- |
T7529 |
25032-25036 |
NN |
denotes |
Pea3 |
T7530 |
25036-25037 |
HYPH |
denotes |
/ |
T7531 |
25037-25038 |
SYM |
denotes |
+ |
T7532 |
25049-25056 |
NNS |
denotes |
embryos |
T7533 |
25046-25047 |
HYPH |
denotes |
/ |
T7534 |
25047-25048 |
SYM |
denotes |
+ |
T7535 |
25061-25063 |
DT |
denotes |
an |
T7536 |
25064-25073 |
NN |
denotes |
influence |
T7537 |
25074-25076 |
IN |
denotes |
on |
T7538 |
25077-25086 |
RB |
denotes |
naturally |
T7539 |
25087-25096 |
VBG |
denotes |
occurring |
T7540 |
25102-25107 |
NN |
denotes |
death |
T7541 |
25097-25101 |
NN |
denotes |
cell |
T7542 |
25108-25110 |
FW |
denotes |
in |
T7543 |
25111-25115 |
FW |
denotes |
vivo |
T7544 |
25116-25121 |
VBG |
denotes |
using |
T7545 |
25122-25127 |
NN |
denotes |
TUNEL |
T7546 |
25128-25130 |
IN |
denotes |
on |
T7547 |
25131-25134 |
NN |
denotes |
DRG |
T7548 |
25135-25143 |
NNS |
denotes |
sections |
T7549 |
25143-25144 |
. |
denotes |
. |
T7550 |
25144-25352 |
sentence |
denotes |
Surprisingly, we found that apoptosis was decreased by approximately 50% (n = 3 embryos, average of >50 sections) in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos in comparison to wild-type (Figure 6D, 6J, and 6M). |
T7551 |
25145-25157 |
RB |
denotes |
Surprisingly |
T7552 |
25162-25167 |
VBD |
denotes |
found |
T7553 |
25157-25159 |
, |
denotes |
, |
T7554 |
25159-25161 |
PRP |
denotes |
we |
T7555 |
25168-25172 |
IN |
denotes |
that |
T7556 |
25187-25196 |
VBN |
denotes |
decreased |
T7557 |
25173-25182 |
NN |
denotes |
apoptosis |
T7558 |
25183-25186 |
VBD |
denotes |
was |
T7559 |
25197-25199 |
IN |
denotes |
by |
T7560 |
25200-25213 |
RB |
denotes |
approximately |
T7561 |
25214-25216 |
CD |
denotes |
50 |
T7562 |
25216-25217 |
NN |
denotes |
% |
T7563 |
25218-25219 |
-LRB- |
denotes |
( |
T7564 |
25234-25241 |
NN |
denotes |
average |
T7565 |
25219-25220 |
NN |
denotes |
n |
T7566 |
25225-25232 |
NNS |
denotes |
embryos |
T7567 |
25221-25222 |
SYM |
denotes |
= |
T7568 |
25223-25224 |
CD |
denotes |
3 |
T7569 |
25232-25234 |
, |
denotes |
, |
T7570 |
25242-25244 |
IN |
denotes |
of |
T7571 |
25245-25246 |
SYM |
denotes |
> |
T7572 |
25246-25248 |
CD |
denotes |
50 |
T7573 |
25249-25257 |
NNS |
denotes |
sections |
T7574 |
25257-25258 |
-RRB- |
denotes |
) |
T7575 |
25259-25261 |
IN |
denotes |
in |
T7576 |
25262-25265 |
NN |
denotes |
DRG |
T7577 |
25266-25268 |
IN |
denotes |
of |
T7578 |
25269-25275 |
NN |
denotes |
TauEWS |
T7579 |
25283-25290 |
NN |
denotes |
Isl1Cre |
T7580 |
25275-25276 |
HYPH |
denotes |
- |
T7581 |
25276-25280 |
NN |
denotes |
Pea3 |
T7582 |
25280-25281 |
HYPH |
denotes |
/ |
T7583 |
25281-25282 |
SYM |
denotes |
+ |
T7584 |
25293-25300 |
NNS |
denotes |
embryos |
T7585 |
25290-25291 |
HYPH |
denotes |
/ |
T7586 |
25291-25292 |
SYM |
denotes |
+ |
T7587 |
25301-25303 |
IN |
denotes |
in |
T7588 |
25304-25314 |
NN |
denotes |
comparison |
T7589 |
25315-25317 |
IN |
denotes |
to |
T7590 |
25318-25322 |
JJ |
denotes |
wild |
T7591 |
25323-25327 |
NN |
denotes |
type |
T7592 |
25322-25323 |
HYPH |
denotes |
- |
T7593 |
25328-25329 |
-LRB- |
denotes |
( |
T7594 |
25336-25338 |
NN |
denotes |
6D |
T7595 |
25329-25335 |
NN |
denotes |
Figure |
T7596 |
25338-25340 |
, |
denotes |
, |
T7597 |
25340-25342 |
NN |
denotes |
6J |
T7598 |
25342-25344 |
, |
denotes |
, |
T7599 |
25344-25347 |
CC |
denotes |
and |
T7600 |
25348-25350 |
NN |
denotes |
6M |
T7601 |
25350-25351 |
-RRB- |
denotes |
) |
T7602 |
25351-25352 |
. |
denotes |
. |
T7603 |
25352-25547 |
sentence |
denotes |
Quantitative analysis of the number of neurons in lumbar DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos revealed a significant increase to approximately 170% of wild-type levels (Figure 6E, 6K, and 6N). |
T7604 |
25353-25365 |
JJ |
denotes |
Quantitative |
T7605 |
25366-25374 |
NN |
denotes |
analysis |
T7606 |
25449-25457 |
VBD |
denotes |
revealed |
T7607 |
25375-25377 |
IN |
denotes |
of |
T7608 |
25378-25381 |
DT |
denotes |
the |
T7609 |
25382-25388 |
NN |
denotes |
number |
T7610 |
25389-25391 |
IN |
denotes |
of |
T7611 |
25392-25399 |
NNS |
denotes |
neurons |
T7612 |
25400-25402 |
IN |
denotes |
in |
T7613 |
25403-25409 |
NN |
denotes |
lumbar |
T7614 |
25410-25413 |
NN |
denotes |
DRG |
T7615 |
25414-25416 |
IN |
denotes |
of |
T7616 |
25417-25423 |
NN |
denotes |
TauEWS |
T7617 |
25431-25438 |
NN |
denotes |
Isl1Cre |
T7618 |
25423-25424 |
HYPH |
denotes |
- |
T7619 |
25424-25428 |
NN |
denotes |
Pea3 |
T7620 |
25428-25429 |
HYPH |
denotes |
/ |
T7621 |
25429-25430 |
SYM |
denotes |
+ |
T7622 |
25441-25448 |
NNS |
denotes |
embryos |
T7623 |
25438-25439 |
HYPH |
denotes |
/ |
T7624 |
25439-25440 |
SYM |
denotes |
+ |
T7625 |
25458-25459 |
DT |
denotes |
a |
T7626 |
25472-25480 |
NN |
denotes |
increase |
T7627 |
25460-25471 |
JJ |
denotes |
significant |
T7628 |
25481-25483 |
IN |
denotes |
to |
T7629 |
25484-25497 |
RB |
denotes |
approximately |
T7630 |
25498-25501 |
CD |
denotes |
170 |
T7631 |
25501-25502 |
NN |
denotes |
% |
T7632 |
25503-25505 |
IN |
denotes |
of |
T7633 |
25506-25510 |
JJ |
denotes |
wild |
T7634 |
25511-25515 |
NN |
denotes |
type |
T7635 |
25510-25511 |
HYPH |
denotes |
- |
T7636 |
25516-25522 |
NNS |
denotes |
levels |
T7637 |
25523-25524 |
-LRB- |
denotes |
( |
T7638 |
25531-25533 |
NN |
denotes |
6E |
T7639 |
25524-25530 |
NN |
denotes |
Figure |
T7640 |
25533-25535 |
, |
denotes |
, |
T7641 |
25535-25537 |
NN |
denotes |
6K |
T7642 |
25537-25539 |
, |
denotes |
, |
T7643 |
25539-25542 |
CC |
denotes |
and |
T7644 |
25543-25545 |
NN |
denotes |
6N |
T7645 |
25545-25546 |
-RRB- |
denotes |
) |
T7646 |
25546-25547 |
. |
denotes |
. |
T7647 |
25547-25775 |
sentence |
denotes |
Moreover, BrdU pulse-chase experiments ruled out the possibility that DRG neurons in TauEWS-Pea3/+ Isl1Cre/+ embryos reenter the cell cycle (no BrdU+/LacZ+ cells, n = 3 embryos, analysis of >50 sections each; Figure 6F and 6L). |
T7648 |
25548-25556 |
RB |
denotes |
Moreover |
T7649 |
25587-25592 |
VBD |
denotes |
ruled |
T7650 |
25556-25558 |
, |
denotes |
, |
T7651 |
25558-25562 |
NN |
denotes |
BrdU |
T7652 |
25575-25586 |
NNS |
denotes |
experiments |
T7653 |
25563-25568 |
NN |
denotes |
pulse |
T7654 |
25569-25574 |
NN |
denotes |
chase |
T7655 |
25568-25569 |
HYPH |
denotes |
- |
T7656 |
25593-25596 |
RP |
denotes |
out |
T7657 |
25597-25600 |
DT |
denotes |
the |
T7658 |
25601-25612 |
NN |
denotes |
possibility |
T7659 |
25613-25617 |
IN |
denotes |
that |
T7660 |
25665-25672 |
VBP |
denotes |
reenter |
T7661 |
25618-25621 |
NN |
denotes |
DRG |
T7662 |
25622-25629 |
NNS |
denotes |
neurons |
T7663 |
25630-25632 |
IN |
denotes |
in |
T7664 |
25633-25639 |
NN |
denotes |
TauEWS |
T7665 |
25647-25654 |
NN |
denotes |
Isl1Cre |
T7666 |
25639-25640 |
HYPH |
denotes |
- |
T7667 |
25640-25644 |
NN |
denotes |
Pea3 |
T7668 |
25644-25645 |
HYPH |
denotes |
/ |
T7669 |
25645-25646 |
SYM |
denotes |
+ |
T7670 |
25657-25664 |
NNS |
denotes |
embryos |
T7671 |
25654-25655 |
HYPH |
denotes |
/ |
T7672 |
25655-25656 |
SYM |
denotes |
+ |
T7673 |
25673-25676 |
DT |
denotes |
the |
T7674 |
25682-25687 |
NN |
denotes |
cycle |
T7675 |
25677-25681 |
NN |
denotes |
cell |
T7676 |
25688-25689 |
-LRB- |
denotes |
( |
T7677 |
25764-25766 |
NN |
denotes |
6F |
T7678 |
25689-25691 |
DT |
denotes |
no |
T7679 |
25704-25709 |
NNS |
denotes |
cells |
T7680 |
25692-25696 |
NN |
denotes |
BrdU |
T7681 |
25698-25702 |
NN |
denotes |
LacZ |
T7682 |
25696-25697 |
SYM |
denotes |
+ |
T7683 |
25697-25698 |
HYPH |
denotes |
/ |
T7684 |
25702-25703 |
SYM |
denotes |
+ |
T7685 |
25709-25711 |
, |
denotes |
, |
T7686 |
25711-25712 |
NN |
denotes |
n |
T7687 |
25717-25724 |
NNS |
denotes |
embryos |
T7688 |
25713-25714 |
SYM |
denotes |
= |
T7689 |
25715-25716 |
CD |
denotes |
3 |
T7690 |
25724-25726 |
, |
denotes |
, |
T7691 |
25726-25734 |
NN |
denotes |
analysis |
T7692 |
25735-25737 |
IN |
denotes |
of |
T7693 |
25738-25739 |
SYM |
denotes |
> |
T7694 |
25739-25741 |
CD |
denotes |
50 |
T7695 |
25742-25750 |
NNS |
denotes |
sections |
T7696 |
25751-25755 |
RB |
denotes |
each |
T7697 |
25755-25756 |
: |
denotes |
; |
T7698 |
25757-25763 |
NN |
denotes |
Figure |
T7699 |
25767-25770 |
CC |
denotes |
and |
T7700 |
25771-25773 |
NN |
denotes |
6L |
T7701 |
25773-25774 |
-RRB- |
denotes |
) |
T7702 |
25774-25775 |
. |
denotes |
. |
T7703 |
25775-26054 |
sentence |
denotes |
Together with the in vitro culture experiments, these findings suggest that DRG neurons from TauEWS-Pea3/+ Isl1Cre/+ embryos remain post-mitotic but fail to become sensitive to naturally occurring cell death, and survive in the absence of Trk receptors and neurotrophic support. |
T7704 |
25776-25784 |
RB |
denotes |
Together |
T7705 |
25839-25846 |
VBP |
denotes |
suggest |
T7706 |
25785-25789 |
IN |
denotes |
with |
T7707 |
25790-25793 |
DT |
denotes |
the |
T7708 |
25811-25822 |
NNS |
denotes |
experiments |
T7709 |
25794-25796 |
FW |
denotes |
in |
T7710 |
25797-25802 |
FW |
denotes |
vitro |
T7711 |
25803-25810 |
NN |
denotes |
culture |
T7712 |
25822-25824 |
, |
denotes |
, |
T7713 |
25824-25829 |
DT |
denotes |
these |
T7714 |
25830-25838 |
NNS |
denotes |
findings |
T7715 |
25847-25851 |
IN |
denotes |
that |
T7716 |
25901-25907 |
VBP |
denotes |
remain |
T7717 |
25852-25855 |
NN |
denotes |
DRG |
T7718 |
25856-25863 |
NNS |
denotes |
neurons |
T7719 |
25864-25868 |
IN |
denotes |
from |
T7720 |
25869-25875 |
NN |
denotes |
TauEWS |
T7721 |
25883-25890 |
NN |
denotes |
Isl1Cre |
T7722 |
25875-25876 |
HYPH |
denotes |
- |
T7723 |
25876-25880 |
NN |
denotes |
Pea3 |
T7724 |
25880-25881 |
HYPH |
denotes |
/ |
T7725 |
25881-25882 |
SYM |
denotes |
+ |
T7726 |
25893-25900 |
NNS |
denotes |
embryos |
T7727 |
25890-25891 |
HYPH |
denotes |
/ |
T7728 |
25891-25892 |
SYM |
denotes |
+ |
T7729 |
25908-25920 |
JJ |
denotes |
post-mitotic |
T7730 |
25921-25924 |
CC |
denotes |
but |
T7731 |
25925-25929 |
VBP |
denotes |
fail |
T7732 |
25930-25932 |
TO |
denotes |
to |
T7733 |
25933-25939 |
VB |
denotes |
become |
T7734 |
25940-25949 |
JJ |
denotes |
sensitive |
T7735 |
25950-25952 |
IN |
denotes |
to |
T7736 |
25953-25962 |
RB |
denotes |
naturally |
T7737 |
25963-25972 |
VBG |
denotes |
occurring |
T7738 |
25978-25983 |
NN |
denotes |
death |
T7739 |
25973-25977 |
NN |
denotes |
cell |
T7740 |
25983-25985 |
, |
denotes |
, |
T7741 |
25985-25988 |
CC |
denotes |
and |
T7742 |
25989-25996 |
VBP |
denotes |
survive |
T7743 |
25997-25999 |
IN |
denotes |
in |
T7744 |
26000-26003 |
DT |
denotes |
the |
T7745 |
26004-26011 |
NN |
denotes |
absence |
T7746 |
26012-26014 |
IN |
denotes |
of |
T7747 |
26015-26018 |
NN |
denotes |
Trk |
T7748 |
26019-26028 |
NNS |
denotes |
receptors |
T7749 |
26029-26032 |
CC |
denotes |
and |
T7750 |
26033-26045 |
JJ |
denotes |
neurotrophic |
T7752 |
26053-26054 |
. |
denotes |
. |
T7753 |
26054-26252 |
sentence |
denotes |
We next analyzed whether changes in the expression of proteins known to be involved in the regulation of neuronal survival or cell death could be detected in DRG of TauEWS-Pea3/+ Isl1Cre/+ embryos. |
T7754 |
26055-26057 |
PRP |
denotes |
We |
T7755 |
26063-26071 |
VBD |
denotes |
analyzed |
T7756 |
26058-26062 |
RB |
denotes |
next |
T7757 |
26072-26079 |
IN |
denotes |
whether |
T7758 |
26201-26209 |
VBN |
denotes |
detected |
T7759 |
26080-26087 |
NNS |
denotes |
changes |
T7760 |
26088-26090 |
IN |
denotes |
in |
T7761 |
26091-26094 |
DT |
denotes |
the |
T7762 |
26095-26105 |
NN |
denotes |
expression |
T7763 |
26106-26108 |
IN |
denotes |
of |
T7764 |
26109-26117 |
NN |
denotes |
proteins |
T7765 |
26118-26123 |
VBN |
denotes |
known |
T7766 |
26124-26126 |
TO |
denotes |
to |
T7767 |
26130-26138 |
VBN |
denotes |
involved |
T7768 |
26127-26129 |
VB |
denotes |
be |
T7769 |
26139-26141 |
IN |
denotes |
in |
T7770 |
26142-26145 |
DT |
denotes |
the |
T7771 |
26146-26156 |
NN |
denotes |
regulation |
T7772 |
26157-26159 |
IN |
denotes |
of |
T7773 |
26160-26168 |
JJ |
denotes |
neuronal |
T7774 |
26169-26177 |
NN |
denotes |
survival |
T7775 |
26178-26180 |
CC |
denotes |
or |
T7776 |
26181-26185 |
NN |
denotes |
cell |
T7777 |
26186-26191 |
NN |
denotes |
death |
T7778 |
26192-26197 |
MD |
denotes |
could |
T7779 |
26198-26200 |
VB |
denotes |
be |
T7780 |
26210-26212 |
IN |
denotes |
in |
T7781 |
26213-26216 |
NN |
denotes |
DRG |
T7782 |
26217-26219 |
IN |
denotes |
of |
T7783 |
26220-26226 |
NN |
denotes |
TauEWS |
T7784 |
26234-26241 |
NN |
denotes |
Isl1Cre |
T7785 |
26226-26227 |
HYPH |
denotes |
- |
T7786 |
26227-26231 |
NN |
denotes |
Pea3 |
T7787 |
26231-26232 |
HYPH |
denotes |
/ |
T7788 |
26232-26233 |
SYM |
denotes |
+ |
T7789 |
26244-26251 |
NNS |
denotes |
embryos |
T7790 |
26241-26242 |
HYPH |
denotes |
/ |
T7791 |
26242-26243 |
SYM |
denotes |
+ |
T7792 |
26251-26252 |
. |
denotes |
. |
T7793 |
26252-26442 |
sentence |
denotes |
We found no significant quantitative changes in the level of Akt/p-Akt or CREB/p-CREB in DRG (Figure 6O and 6P) both of which have been shown to be key regulators of neuronal survival [29]. |
T7794 |
26253-26255 |
PRP |
denotes |
We |
T7795 |
26256-26261 |
VBD |
denotes |
found |
T7796 |
26262-26264 |
DT |
denotes |
no |
T7797 |
26290-26297 |
NNS |
denotes |
changes |
T7798 |
26265-26276 |
JJ |
denotes |
significant |
T7799 |
26277-26289 |
JJ |
denotes |
quantitative |
T7800 |
26298-26300 |
IN |
denotes |
in |
T7801 |
26301-26304 |
DT |
denotes |
the |
T7802 |
26305-26310 |
NN |
denotes |
level |
T7803 |
26311-26313 |
IN |
denotes |
of |
T7804 |
26314-26317 |
NN |
denotes |
Akt |
T7805 |
26320-26323 |
NN |
denotes |
Akt |
T7806 |
26317-26318 |
HYPH |
denotes |
/ |
T7807 |
26318-26319 |
NN |
denotes |
p |
T7808 |
26319-26320 |
HYPH |
denotes |
- |
T7809 |
26324-26326 |
CC |
denotes |
or |
T7810 |
26327-26331 |
NN |
denotes |
CREB |
T7811 |
26334-26338 |
NN |
denotes |
CREB |
T7812 |
26331-26332 |
HYPH |
denotes |
/ |
T7813 |
26332-26333 |
NN |
denotes |
p |
T7814 |
26333-26334 |
HYPH |
denotes |
- |
T7815 |
26339-26341 |
IN |
denotes |
in |
T7816 |
26342-26345 |
NN |
denotes |
DRG |
T7817 |
26346-26347 |
-LRB- |
denotes |
( |
T7818 |
26354-26356 |
NN |
denotes |
6O |
T7819 |
26347-26353 |
NN |
denotes |
Figure |
T7820 |
26357-26360 |
CC |
denotes |
and |
T7821 |
26361-26363 |
NN |
denotes |
6P |
T7822 |
26363-26364 |
-RRB- |
denotes |
) |
T7823 |
26365-26369 |
DT |
denotes |
both |
T7824 |
26389-26394 |
VBN |
denotes |
shown |
T7825 |
26370-26372 |
IN |
denotes |
of |
T7826 |
26373-26378 |
WDT |
denotes |
which |
T7827 |
26379-26383 |
VBP |
denotes |
have |
T7828 |
26384-26388 |
VBN |
denotes |
been |
T7829 |
26395-26397 |
TO |
denotes |
to |
T7830 |
26398-26400 |
VB |
denotes |
be |
T7831 |
26401-26404 |
JJ |
denotes |
key |
T7832 |
26405-26415 |
NNS |
denotes |
regulators |
T7833 |
26416-26418 |
IN |
denotes |
of |
T7834 |
26419-26427 |
JJ |
denotes |
neuronal |
T7835 |
26428-26436 |
NN |
denotes |
survival |
T7836 |
26437-26438 |
-LRB- |
denotes |
[ |
T7837 |
26438-26440 |
CD |
denotes |
29 |
T7838 |
26440-26441 |
-RRB- |
denotes |
] |
T7839 |
26441-26442 |
. |
denotes |
. |
T7840 |
26442-26555 |
sentence |
denotes |
Moreover, the level of the proapoptotic Bcl2 family member Bax was not significantly reduced (Figure 6O and 6P). |
T7841 |
26443-26451 |
RB |
denotes |
Moreover |
T7842 |
26528-26535 |
VBN |
denotes |
reduced |
T7843 |
26451-26453 |
, |
denotes |
, |
T7844 |
26453-26456 |
DT |
denotes |
the |
T7845 |
26457-26462 |
NN |
denotes |
level |
T7846 |
26463-26465 |
IN |
denotes |
of |
T7847 |
26466-26469 |
DT |
denotes |
the |
T7848 |
26502-26505 |
NN |
denotes |
Bax |
T7849 |
26470-26482 |
JJ |
denotes |
proapoptotic |
T7850 |
26483-26487 |
NN |
denotes |
Bcl2 |
T7851 |
26488-26494 |
NN |
denotes |
family |
T7852 |
26495-26501 |
NN |
denotes |
member |
T7853 |
26506-26509 |
VBD |
denotes |
was |
T7854 |
26510-26513 |
RB |
denotes |
not |
T7855 |
26514-26527 |
RB |
denotes |
significantly |
T7856 |
26536-26537 |
-LRB- |
denotes |
( |
T7857 |
26544-26546 |
NN |
denotes |
6O |
T7858 |
26537-26543 |
NN |
denotes |
Figure |
T7859 |
26547-26550 |
CC |
denotes |
and |
T7860 |
26551-26553 |
NN |
denotes |
6P |
T7861 |
26553-26554 |
-RRB- |
denotes |
) |
T7862 |
26554-26555 |
. |
denotes |
. |
T7863 |
26555-26974 |
sentence |
denotes |
In contrast, the expression level of the anti-apoptotic Bcl2 family members Bcl-xl and Bcl2 was significantly increased when compared to wild-type levels (Bcl2, 157%; Bcl-xl, 259%; average of n = 3 independent experiments; Figure 6O and 6P), providing a potential molecular explanation for the enhanced neuronal survival of DRG neurons of TauEWS-Pea3/+ Isl1Cre/+ embryos in the absence of Trk receptor expression [30]. |
T7864 |
26556-26558 |
IN |
denotes |
In |
T7865 |
26666-26675 |
VBN |
denotes |
increased |
T7866 |
26559-26567 |
NN |
denotes |
contrast |
T7867 |
26567-26569 |
, |
denotes |
, |
T7868 |
26569-26572 |
DT |
denotes |
the |
T7869 |
26584-26589 |
NN |
denotes |
level |
T7870 |
26573-26583 |
NN |
denotes |
expression |
T7871 |
26590-26592 |
IN |
denotes |
of |
T7872 |
26593-26596 |
DT |
denotes |
the |
T7873 |
26624-26631 |
NNS |
denotes |
members |
T7874 |
26597-26611 |
JJ |
denotes |
anti-apoptotic |
T7875 |
26612-26616 |
NN |
denotes |
Bcl2 |
T7876 |
26617-26623 |
NN |
denotes |
family |
T7877 |
26632-26635 |
NN |
denotes |
Bcl |
T7878 |
26636-26638 |
NN |
denotes |
xl |
T7879 |
26635-26636 |
HYPH |
denotes |
- |
T7880 |
26639-26642 |
CC |
denotes |
and |
T7881 |
26643-26647 |
NN |
denotes |
Bcl2 |
T7882 |
26648-26651 |
VBD |
denotes |
was |
T7883 |
26652-26665 |
RB |
denotes |
significantly |
T7884 |
26676-26680 |
WRB |
denotes |
when |
T7885 |
26681-26689 |
VBN |
denotes |
compared |
T7886 |
26690-26692 |
IN |
denotes |
to |
T7887 |
26693-26697 |
JJ |
denotes |
wild |
T7888 |
26698-26702 |
NN |
denotes |
type |
T7889 |
26697-26698 |
HYPH |
denotes |
- |
T7890 |
26703-26709 |
NNS |
denotes |
levels |
T7891 |
26710-26711 |
-LRB- |
denotes |
( |
T7892 |
26786-26788 |
NN |
denotes |
6O |
T7893 |
26711-26715 |
NN |
denotes |
Bcl2 |
T7894 |
26720-26721 |
NN |
denotes |
% |
T7895 |
26715-26717 |
, |
denotes |
, |
T7896 |
26717-26720 |
CD |
denotes |
157 |
T7897 |
26721-26722 |
: |
denotes |
; |
T7898 |
26723-26726 |
NN |
denotes |
Bcl |
T7899 |
26727-26729 |
NN |
denotes |
xl |
T7900 |
26726-26727 |
HYPH |
denotes |
- |
T7901 |
26734-26735 |
NN |
denotes |
% |
T7902 |
26729-26731 |
, |
denotes |
, |
T7903 |
26731-26734 |
CD |
denotes |
259 |
T7904 |
26735-26736 |
: |
denotes |
; |
T7905 |
26737-26744 |
NN |
denotes |
average |
T7906 |
26745-26747 |
IN |
denotes |
of |
T7907 |
26748-26749 |
NN |
denotes |
n |
T7908 |
26766-26777 |
NNS |
denotes |
experiments |
T7909 |
26750-26751 |
SYM |
denotes |
= |
T7910 |
26752-26753 |
CD |
denotes |
3 |
T7911 |
26754-26765 |
JJ |
denotes |
independent |
T7912 |
26777-26778 |
: |
denotes |
; |
T7913 |
26779-26785 |
NN |
denotes |
Figure |
T7914 |
26789-26792 |
CC |
denotes |
and |
T7915 |
26793-26795 |
NN |
denotes |
6P |
T7916 |
26795-26796 |
-RRB- |
denotes |
) |
T7917 |
26796-26798 |
, |
denotes |
, |
T7918 |
26798-26807 |
VBG |
denotes |
providing |
T7919 |
26808-26809 |
DT |
denotes |
a |
T7920 |
26830-26841 |
NN |
denotes |
explanation |
T7921 |
26810-26819 |
JJ |
denotes |
potential |
T7922 |
26820-26829 |
JJ |
denotes |
molecular |
T7923 |
26842-26845 |
IN |
denotes |
for |
T7924 |
26846-26849 |
DT |
denotes |
the |
T7925 |
26868-26876 |
NN |
denotes |
survival |
T7926 |
26850-26858 |
VBN |
denotes |
enhanced |
T7927 |
26859-26867 |
JJ |
denotes |
neuronal |
T7928 |
26877-26879 |
IN |
denotes |
of |
T7929 |
26880-26883 |
NN |
denotes |
DRG |
T7930 |
26884-26891 |
NNS |
denotes |
neurons |
T7931 |
26892-26894 |
IN |
denotes |
of |
T7932 |
26895-26901 |
NN |
denotes |
TauEWS |
T7933 |
26909-26916 |
NN |
denotes |
Isl1Cre |
T7934 |
26901-26902 |
HYPH |
denotes |
- |
T7935 |
26902-26906 |
NN |
denotes |
Pea3 |
T7936 |
26906-26907 |
HYPH |
denotes |
/ |
T7937 |
26907-26908 |
SYM |
denotes |
+ |
T7938 |
26919-26926 |
NNS |
denotes |
embryos |
T7939 |
26916-26917 |
HYPH |
denotes |
/ |
T7940 |
26917-26918 |
SYM |
denotes |
+ |
T7941 |
26927-26929 |
IN |
denotes |
in |
T7942 |
26930-26933 |
DT |
denotes |
the |
T7943 |
26934-26941 |
NN |
denotes |
absence |
T7944 |
26942-26944 |
IN |
denotes |
of |
T7945 |
26945-26948 |
NN |
denotes |
Trk |
T7946 |
26949-26957 |
NN |
denotes |
receptor |
T7947 |
26958-26968 |
NN |
denotes |
expression |
T7948 |
26969-26970 |
-LRB- |
denotes |
[ |
T7949 |
26970-26972 |
CD |
denotes |
30 |
T7950 |
26972-26973 |
-RRB- |
denotes |
] |
T7951 |
26973-26974 |
. |
denotes |
. |
T8705 |
26976-26980 |
RB |
denotes |
Only |
T8706 |
27015-27024 |
NN |
denotes |
Signaling |
T8707 |
26981-26991 |
JJ |
denotes |
Precocious |
T8708 |
26992-26995 |
CC |
denotes |
but |
T8709 |
26996-26999 |
RB |
denotes |
Not |
T8710 |
27000-27010 |
JJ |
denotes |
Isochronic |
T8711 |
27011-27014 |
NN |
denotes |
ETS |
T8712 |
27040-27050 |
VBZ |
denotes |
Interferes |
T8713 |
27025-27027 |
IN |
denotes |
in |
T8714 |
27028-27031 |
NN |
denotes |
DRG |
T8715 |
27032-27039 |
NNS |
denotes |
Neurons |
T8716 |
27051-27055 |
IN |
denotes |
with |
T8717 |
27056-27064 |
JJ |
denotes |
Neuronal |
T8718 |
27065-27069 |
NN |
denotes |
Fate |
T8719 |
27070-27081 |
NN |
denotes |
Acquisition |
T8720 |
27081-27458 |
sentence |
denotes |
The observed differences in neuronal survival and neurite outgrowth between precocious and isochronic expression of EWS-Pea3 prompted us to perform a direct comparative analysis of gene expression between mice with precocious EWS-Pea3 expression and mice in which the expression of EWS-Pea3 is initiated in DRG sensory neurons from the time of normal onset of Er81 expression. |
T8721 |
27082-27085 |
DT |
denotes |
The |
T8722 |
27095-27106 |
NNS |
denotes |
differences |
T8723 |
27086-27094 |
VBN |
denotes |
observed |
T8724 |
27207-27215 |
VBD |
denotes |
prompted |
T8725 |
27107-27109 |
IN |
denotes |
in |
T8726 |
27110-27118 |
JJ |
denotes |
neuronal |
T8727 |
27119-27127 |
NN |
denotes |
survival |
T8728 |
27128-27131 |
CC |
denotes |
and |
T8729 |
27132-27139 |
NN |
denotes |
neurite |
T8730 |
27140-27149 |
NN |
denotes |
outgrowth |
T8731 |
27150-27157 |
IN |
denotes |
between |
T8732 |
27158-27168 |
JJ |
denotes |
precocious |
T8733 |
27184-27194 |
NN |
denotes |
expression |
T8734 |
27169-27172 |
CC |
denotes |
and |
T8735 |
27173-27183 |
JJ |
denotes |
isochronic |
T8736 |
27195-27197 |
IN |
denotes |
of |
T8737 |
27198-27201 |
NN |
denotes |
EWS |
T8738 |
27202-27206 |
NN |
denotes |
Pea3 |
T8739 |
27201-27202 |
HYPH |
denotes |
- |
T8740 |
27216-27218 |
PRP |
denotes |
us |
T8741 |
27219-27221 |
TO |
denotes |
to |
T8742 |
27222-27229 |
VB |
denotes |
perform |
T8743 |
27230-27231 |
DT |
denotes |
a |
T8744 |
27251-27259 |
NN |
denotes |
analysis |
T8745 |
27232-27238 |
JJ |
denotes |
direct |
T8746 |
27239-27250 |
JJ |
denotes |
comparative |
T8747 |
27260-27262 |
IN |
denotes |
of |
T8748 |
27263-27267 |
NN |
denotes |
gene |
T8749 |
27268-27278 |
NN |
denotes |
expression |
T8750 |
27279-27286 |
IN |
denotes |
between |
T8751 |
27287-27291 |
NNS |
denotes |
mice |
T8752 |
27292-27296 |
IN |
denotes |
with |
T8753 |
27297-27307 |
JJ |
denotes |
precocious |
T8754 |
27317-27327 |
NN |
denotes |
expression |
T8755 |
27308-27311 |
NN |
denotes |
EWS |
T8756 |
27312-27316 |
NN |
denotes |
Pea3 |
T8757 |
27311-27312 |
HYPH |
denotes |
- |
T8758 |
27328-27331 |
CC |
denotes |
and |
T8759 |
27332-27336 |
NNS |
denotes |
mice |
T8760 |
27337-27339 |
IN |
denotes |
in |
T8761 |
27376-27385 |
VBN |
denotes |
initiated |
T8762 |
27340-27345 |
WDT |
denotes |
which |
T8763 |
27346-27349 |
DT |
denotes |
the |
T8764 |
27350-27360 |
NN |
denotes |
expression |
T8765 |
27361-27363 |
IN |
denotes |
of |
T8766 |
27364-27367 |
NN |
denotes |
EWS |
T8767 |
27368-27372 |
NN |
denotes |
Pea3 |
T8768 |
27367-27368 |
HYPH |
denotes |
- |
T8769 |
27373-27375 |
VBZ |
denotes |
is |
T8770 |
27386-27388 |
IN |
denotes |
in |
T8771 |
27389-27392 |
NN |
denotes |
DRG |
T8772 |
27401-27408 |
NNS |
denotes |
neurons |
T8773 |
27393-27400 |
JJ |
denotes |
sensory |
T8774 |
27409-27413 |
IN |
denotes |
from |
T8775 |
27414-27417 |
DT |
denotes |
the |
T8776 |
27418-27422 |
NN |
denotes |
time |
T8777 |
27423-27425 |
IN |
denotes |
of |
T8778 |
27426-27432 |
JJ |
denotes |
normal |
T8779 |
27433-27438 |
NN |
denotes |
onset |
T8780 |
27439-27441 |
IN |
denotes |
of |
T8781 |
27442-27446 |
NN |
denotes |
Er81 |
T8782 |
27447-27457 |
NN |
denotes |
expression |
T8783 |
27457-27458 |
. |
denotes |
. |
T8784 |
27458-27728 |
sentence |
denotes |
Moreover, to rule out the possibility that a differential effect may be due to the different genetic strategies by which expression of EWS-Pea3 in proprioceptive afferents is achieved, we performed this analysis both in Er81EWS-Pea3/− and TauEWS-Pea3/+ PVCre/+ embryos. |
T8785 |
27459-27467 |
RB |
denotes |
Moreover |
T8786 |
27647-27656 |
VBD |
denotes |
performed |
T8787 |
27467-27469 |
, |
denotes |
, |
T8788 |
27469-27471 |
TO |
denotes |
to |
T8789 |
27472-27476 |
VB |
denotes |
rule |
T8790 |
27477-27480 |
RP |
denotes |
out |
T8791 |
27481-27484 |
DT |
denotes |
the |
T8792 |
27485-27496 |
NN |
denotes |
possibility |
T8793 |
27497-27501 |
IN |
denotes |
that |
T8794 |
27528-27530 |
VB |
denotes |
be |
T8795 |
27502-27503 |
DT |
denotes |
a |
T8796 |
27517-27523 |
NN |
denotes |
effect |
T8797 |
27504-27516 |
JJ |
denotes |
differential |
T8798 |
27524-27527 |
MD |
denotes |
may |
T8799 |
27531-27534 |
IN |
denotes |
due |
T8800 |
27535-27537 |
IN |
denotes |
to |
T8801 |
27538-27541 |
DT |
denotes |
the |
T8802 |
27560-27570 |
NNS |
denotes |
strategies |
T8803 |
27542-27551 |
JJ |
denotes |
different |
T8804 |
27552-27559 |
JJ |
denotes |
genetic |
T8805 |
27571-27573 |
IN |
denotes |
by |
T8806 |
27634-27642 |
VBN |
denotes |
achieved |
T8807 |
27574-27579 |
WDT |
denotes |
which |
T8808 |
27580-27590 |
NN |
denotes |
expression |
T8809 |
27591-27593 |
IN |
denotes |
of |
T8810 |
27594-27597 |
NN |
denotes |
EWS |
T8811 |
27598-27602 |
NN |
denotes |
Pea3 |
T8812 |
27597-27598 |
HYPH |
denotes |
- |
T8813 |
27603-27605 |
IN |
denotes |
in |
T8814 |
27606-27620 |
JJ |
denotes |
proprioceptive |
T8815 |
27621-27630 |
NNS |
denotes |
afferents |
T8816 |
27631-27633 |
VBZ |
denotes |
is |
T8817 |
27642-27644 |
, |
denotes |
, |
T8818 |
27644-27646 |
PRP |
denotes |
we |
T8819 |
27657-27661 |
DT |
denotes |
this |
T8820 |
27662-27670 |
NN |
denotes |
analysis |
T8821 |
27671-27675 |
CC |
denotes |
both |
T8822 |
27676-27678 |
IN |
denotes |
in |
T8823 |
27679-27686 |
NN |
denotes |
Er81EWS |
T8824 |
27687-27691 |
NN |
denotes |
Pea3 |
T8825 |
27686-27687 |
HYPH |
denotes |
- |
T8826 |
27720-27727 |
NNS |
denotes |
embryos |
T8827 |
27691-27692 |
HYPH |
denotes |
/ |
T8828 |
27692-27693 |
SYM |
denotes |
− |
T8829 |
27694-27697 |
CC |
denotes |
and |
T8830 |
27698-27704 |
NN |
denotes |
TauEWS |
T8831 |
27712-27717 |
NN |
denotes |
PVCre |
T8832 |
27704-27705 |
HYPH |
denotes |
- |
T8833 |
27705-27709 |
NN |
denotes |
Pea3 |
T8834 |
27709-27710 |
HYPH |
denotes |
/ |
T8835 |
27710-27711 |
SYM |
denotes |
+ |
T8836 |
27717-27718 |
HYPH |
denotes |
/ |
T8837 |
27718-27719 |
SYM |
denotes |
+ |
T8838 |
27727-27728 |
. |
denotes |
. |
T8839 |
27728-27857 |
sentence |
denotes |
We first analyzed expression of TrkC, a gene downregulated in DRG neurons of TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure 7A and 7B). |
T8840 |
27729-27731 |
PRP |
denotes |
We |
T8841 |
27738-27746 |
VBD |
denotes |
analyzed |
T8842 |
27732-27737 |
RB |
denotes |
first |
T8843 |
27747-27757 |
NN |
denotes |
expression |
T8844 |
27758-27760 |
IN |
denotes |
of |
T8845 |
27761-27765 |
NN |
denotes |
TrkC |
T8846 |
27765-27767 |
, |
denotes |
, |
T8847 |
27767-27768 |
DT |
denotes |
a |
T8848 |
27769-27773 |
NN |
denotes |
gene |
T8849 |
27774-27787 |
VBN |
denotes |
downregulated |
T8850 |
27788-27790 |
IN |
denotes |
in |
T8851 |
27791-27794 |
NN |
denotes |
DRG |
T8852 |
27795-27802 |
NNS |
denotes |
neurons |
T8853 |
27803-27805 |
IN |
denotes |
of |
T8854 |
27806-27812 |
NN |
denotes |
TauEWS |
T8855 |
27820-27827 |
NN |
denotes |
Isl1Cre |
T8856 |
27812-27813 |
HYPH |
denotes |
- |
T8857 |
27813-27817 |
NN |
denotes |
Pea3 |
T8858 |
27817-27818 |
HYPH |
denotes |
/ |
T8859 |
27818-27819 |
SYM |
denotes |
+ |
T8860 |
27830-27837 |
NNS |
denotes |
embryos |
T8861 |
27827-27828 |
HYPH |
denotes |
/ |
T8862 |
27828-27829 |
SYM |
denotes |
+ |
T8863 |
27838-27839 |
-LRB- |
denotes |
( |
T8864 |
27846-27848 |
NN |
denotes |
7A |
T8865 |
27839-27845 |
NN |
denotes |
Figure |
T8866 |
27849-27852 |
CC |
denotes |
and |
T8867 |
27853-27855 |
NN |
denotes |
7B |
T8868 |
27855-27856 |
-RRB- |
denotes |
) |
T8869 |
27856-27857 |
. |
denotes |
. |
T8870 |
27857-28018 |
sentence |
denotes |
The level of expression of TrkC was indistinguishable from wild-type in DRG neurons of Er81EWS-Pea3/− and TauEWS-Pea3/+ PVCre/+ embryos (Figure 7A, 7C, and 7D). |
T8871 |
27858-27861 |
DT |
denotes |
The |
T8872 |
27862-27867 |
NN |
denotes |
level |
T8873 |
27890-27893 |
VBD |
denotes |
was |
T8874 |
27868-27870 |
IN |
denotes |
of |
T8875 |
27871-27881 |
NN |
denotes |
expression |
T8876 |
27882-27884 |
IN |
denotes |
of |
T8877 |
27885-27889 |
NN |
denotes |
TrkC |
T8878 |
27894-27911 |
JJ |
denotes |
indistinguishable |
T8879 |
27912-27916 |
IN |
denotes |
from |
T8880 |
27917-27921 |
JJ |
denotes |
wild |
T8881 |
27922-27926 |
NN |
denotes |
type |
T8882 |
27921-27922 |
HYPH |
denotes |
- |
T8883 |
27927-27929 |
IN |
denotes |
in |
T8884 |
27930-27933 |
NN |
denotes |
DRG |
T8885 |
27934-27941 |
NNS |
denotes |
neurons |
T8886 |
27942-27944 |
IN |
denotes |
of |
T8887 |
27945-27952 |
NN |
denotes |
Er81EWS |
T8888 |
27953-27957 |
NN |
denotes |
Pea3 |
T8889 |
27952-27953 |
HYPH |
denotes |
- |
T8890 |
27986-27993 |
NNS |
denotes |
embryos |
T8891 |
27957-27958 |
HYPH |
denotes |
/ |
T8892 |
27958-27959 |
SYM |
denotes |
− |
T8893 |
27960-27963 |
CC |
denotes |
and |
T8894 |
27964-27970 |
NN |
denotes |
TauEWS |
T8895 |
27978-27983 |
NN |
denotes |
PVCre |
T8896 |
27970-27971 |
HYPH |
denotes |
- |
T8897 |
27971-27975 |
NN |
denotes |
Pea3 |
T8898 |
27975-27976 |
HYPH |
denotes |
/ |
T8899 |
27976-27977 |
SYM |
denotes |
+ |
T8900 |
27983-27984 |
HYPH |
denotes |
/ |
T8901 |
27984-27985 |
SYM |
denotes |
+ |
T8902 |
27994-27995 |
-LRB- |
denotes |
( |
T8903 |
28002-28004 |
NN |
denotes |
7A |
T8904 |
27995-28001 |
NN |
denotes |
Figure |
T8905 |
28004-28006 |
, |
denotes |
, |
T8906 |
28006-28008 |
NN |
denotes |
7C |
T8907 |
28008-28010 |
, |
denotes |
, |
T8908 |
28010-28013 |
CC |
denotes |
and |
T8909 |
28014-28016 |
NN |
denotes |
7D |
T8910 |
28016-28017 |
-RRB- |
denotes |
) |
T8911 |
28017-28018 |
. |
denotes |
. |
T8912 |
28018-28231 |
sentence |
denotes |
Moreover, PV was not expressed in DRG neurons of TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure S5) but was expressed by proprioceptive afferents in both wild-type and Er81EWS-Pea3/− embryos (see Figures 1 and S5) [14]. |
T8913 |
28019-28027 |
RB |
denotes |
Moreover |
T8914 |
28040-28049 |
VBN |
denotes |
expressed |
T8915 |
28027-28029 |
, |
denotes |
, |
T8916 |
28029-28031 |
NN |
denotes |
PV |
T8917 |
28032-28035 |
VBD |
denotes |
was |
T8918 |
28036-28039 |
RB |
denotes |
not |
T8919 |
28050-28052 |
IN |
denotes |
in |
T8920 |
28053-28056 |
NN |
denotes |
DRG |
T8921 |
28057-28064 |
NNS |
denotes |
neurons |
T8922 |
28065-28067 |
IN |
denotes |
of |
T8923 |
28068-28074 |
NN |
denotes |
TauEWS |
T8924 |
28082-28089 |
NN |
denotes |
Isl1Cre |
T8925 |
28074-28075 |
HYPH |
denotes |
- |
T8926 |
28075-28079 |
NN |
denotes |
Pea3 |
T8927 |
28079-28080 |
HYPH |
denotes |
/ |
T8928 |
28080-28081 |
SYM |
denotes |
+ |
T8929 |
28092-28099 |
NNS |
denotes |
embryos |
T8930 |
28089-28090 |
HYPH |
denotes |
/ |
T8931 |
28090-28091 |
SYM |
denotes |
+ |
T8932 |
28100-28101 |
-LRB- |
denotes |
( |
T8933 |
28108-28110 |
NN |
denotes |
S5 |
T8934 |
28101-28107 |
NN |
denotes |
Figure |
T8935 |
28110-28111 |
-RRB- |
denotes |
) |
T8936 |
28112-28115 |
CC |
denotes |
but |
T8937 |
28116-28119 |
VBD |
denotes |
was |
T8938 |
28120-28129 |
VBN |
denotes |
expressed |
T8939 |
28130-28132 |
IN |
denotes |
by |
T8940 |
28133-28147 |
JJ |
denotes |
proprioceptive |
T8941 |
28148-28157 |
NNS |
denotes |
afferents |
T8942 |
28158-28160 |
IN |
denotes |
in |
T8943 |
28161-28165 |
CC |
denotes |
both |
T8944 |
28171-28175 |
NN |
denotes |
type |
T8945 |
28166-28170 |
JJ |
denotes |
wild |
T8946 |
28170-28171 |
HYPH |
denotes |
- |
T8947 |
28195-28202 |
NNS |
denotes |
embryos |
T8948 |
28176-28179 |
CC |
denotes |
and |
T8949 |
28180-28187 |
NN |
denotes |
Er81EWS |
T8950 |
28188-28192 |
NN |
denotes |
Pea3 |
T8951 |
28187-28188 |
HYPH |
denotes |
- |
T8952 |
28192-28193 |
HYPH |
denotes |
/ |
T8953 |
28193-28194 |
SYM |
denotes |
− |
T8954 |
28203-28204 |
-LRB- |
denotes |
( |
T8955 |
28204-28207 |
VB |
denotes |
see |
T8956 |
28208-28215 |
NNS |
denotes |
Figures |
T8957 |
28222-28224 |
NN |
denotes |
S5 |
T8958 |
28216-28217 |
CD |
denotes |
1 |
T8959 |
28218-28221 |
CC |
denotes |
and |
T8960 |
28224-28225 |
-RRB- |
denotes |
) |
T8961 |
28226-28227 |
-LRB- |
denotes |
[ |
T8962 |
28227-28229 |
CD |
denotes |
14 |
T8963 |
28229-28230 |
-RRB- |
denotes |
] |
T8964 |
28230-28231 |
. |
denotes |
. |
T8965 |
28231-28355 |
sentence |
denotes |
We also found several genes that were ectopically upregulated in DRG neurons of TauEWS-Pea3/+ Isl1Cre/+ embryos (Figure 7). |
T8966 |
28232-28234 |
PRP |
denotes |
We |
T8967 |
28240-28245 |
VBD |
denotes |
found |
T8968 |
28235-28239 |
RB |
denotes |
also |
T8969 |
28246-28253 |
JJ |
denotes |
several |
T8970 |
28254-28259 |
NNS |
denotes |
genes |
T8971 |
28260-28264 |
WDT |
denotes |
that |
T8972 |
28282-28293 |
VBN |
denotes |
upregulated |
T8973 |
28265-28269 |
VBD |
denotes |
were |
T8974 |
28270-28281 |
RB |
denotes |
ectopically |
T8975 |
28294-28296 |
IN |
denotes |
in |
T8976 |
28297-28300 |
NN |
denotes |
DRG |
T8977 |
28301-28308 |
NNS |
denotes |
neurons |
T8978 |
28309-28311 |
IN |
denotes |
of |
T8979 |
28312-28318 |
NN |
denotes |
TauEWS |
T8980 |
28326-28333 |
NN |
denotes |
Isl1Cre |
T8981 |
28318-28319 |
HYPH |
denotes |
- |
T8982 |
28319-28323 |
NN |
denotes |
Pea3 |
T8983 |
28323-28324 |
HYPH |
denotes |
/ |
T8984 |
28324-28325 |
SYM |
denotes |
+ |
T8985 |
28336-28343 |
NNS |
denotes |
embryos |
T8986 |
28333-28334 |
HYPH |
denotes |
/ |
T8987 |
28334-28335 |
SYM |
denotes |
+ |
T8988 |
28344-28345 |
-LRB- |
denotes |
( |
T8989 |
28345-28351 |
NN |
denotes |
Figure |
T8990 |
28352-28353 |
CD |
denotes |
7 |
T8991 |
28353-28354 |
-RRB- |
denotes |
) |
T8992 |
28354-28355 |
. |
denotes |
. |
T8993 |
28355-28681 |
sentence |
denotes |
Calretinin and Calbindin, two different calcium-binding proteins expressed by subpopulations of DRG neurons in wild-type, Er81EWS-Pea3/−, and TauEWS-Pea3/+ PVCre/+ embryos (Figure 7E, 7G, and 7H; data not shown) [31,32], were induced in more than 95% of all DRG neurons of TauEWS-Pea3/+ Isl1Cre/+ embryos (Figures 7F and S5). |
T8994 |
28356-28366 |
NN |
denotes |
Calretinin |
T8995 |
28582-28589 |
VBN |
denotes |
induced |
T8996 |
28367-28370 |
CC |
denotes |
and |
T8997 |
28371-28380 |
NN |
denotes |
Calbindin |
T8998 |
28380-28382 |
, |
denotes |
, |
T8999 |
28382-28385 |
CD |
denotes |
two |
T9000 |
28412-28420 |
NN |
denotes |
proteins |
T9001 |
28386-28395 |
JJ |
denotes |
different |
T9002 |
28396-28403 |
NN |
denotes |
calcium |
T9003 |
28404-28411 |
VBG |
denotes |
binding |
T9004 |
28403-28404 |
HYPH |
denotes |
- |
T9005 |
28421-28430 |
VBN |
denotes |
expressed |
T9006 |
28431-28433 |
IN |
denotes |
by |
T9007 |
28434-28448 |
NNS |
denotes |
subpopulations |
T9008 |
28449-28451 |
IN |
denotes |
of |
T9009 |
28452-28455 |
NN |
denotes |
DRG |
T9010 |
28456-28463 |
NNS |
denotes |
neurons |
T9011 |
28464-28466 |
IN |
denotes |
in |
T9012 |
28467-28471 |
JJ |
denotes |
wild |
T9013 |
28472-28476 |
NN |
denotes |
type |
T9014 |
28471-28472 |
HYPH |
denotes |
- |
T9015 |
28520-28527 |
NNS |
denotes |
embryos |
T9016 |
28476-28478 |
, |
denotes |
, |
T9017 |
28478-28485 |
NN |
denotes |
Er81EWS |
T9018 |
28486-28490 |
NN |
denotes |
Pea3 |
T9019 |
28485-28486 |
HYPH |
denotes |
- |
T9020 |
28490-28491 |
HYPH |
denotes |
/ |
T9021 |
28491-28492 |
SYM |
denotes |
− |
T9022 |
28492-28494 |
, |
denotes |
, |
T9023 |
28494-28497 |
CC |
denotes |
and |
T9024 |
28498-28504 |
NN |
denotes |
TauEWS |
T9025 |
28512-28517 |
NN |
denotes |
PVCre |
T9026 |
28504-28505 |
HYPH |
denotes |
- |
T9027 |
28505-28509 |
NN |
denotes |
Pea3 |
T9028 |
28509-28510 |
HYPH |
denotes |
/ |
T9029 |
28510-28511 |
SYM |
denotes |
+ |
T9030 |
28517-28518 |
HYPH |
denotes |
/ |
T9031 |
28518-28519 |
SYM |
denotes |
+ |
T9032 |
28528-28529 |
-LRB- |
denotes |
( |
T9033 |
28536-28538 |
NN |
denotes |
7E |
T9034 |
28529-28535 |
NN |
denotes |
Figure |
T9035 |
28538-28540 |
, |
denotes |
, |
T9036 |
28540-28542 |
NN |
denotes |
7G |
T9037 |
28542-28544 |
, |
denotes |
, |
T9038 |
28544-28547 |
CC |
denotes |
and |
T9039 |
28548-28550 |
NN |
denotes |
7H |
T9040 |
28550-28551 |
: |
denotes |
; |
T9041 |
28552-28556 |
NNS |
denotes |
data |
T9042 |
28561-28566 |
VBN |
denotes |
shown |
T9043 |
28557-28560 |
RB |
denotes |
not |
T9044 |
28566-28567 |
-RRB- |
denotes |
) |
T9045 |
28568-28569 |
-LRB- |
denotes |
[ |
T9046 |
28572-28574 |
CD |
denotes |
32 |
T9047 |
28569-28571 |
CD |
denotes |
31 |
T9048 |
28571-28572 |
, |
denotes |
, |
T9049 |
28574-28575 |
-RRB- |
denotes |
] |
T9050 |
28575-28577 |
, |
denotes |
, |
T9051 |
28577-28581 |
VBD |
denotes |
were |
T9052 |
28590-28592 |
IN |
denotes |
in |
T9053 |
28593-28597 |
JJR |
denotes |
more |
T9054 |
28603-28605 |
CD |
denotes |
95 |
T9055 |
28598-28602 |
IN |
denotes |
than |
T9056 |
28605-28606 |
NN |
denotes |
% |
T9057 |
28607-28609 |
IN |
denotes |
of |
T9058 |
28610-28613 |
DT |
denotes |
all |
T9059 |
28618-28625 |
NNS |
denotes |
neurons |
T9060 |
28614-28617 |
NN |
denotes |
DRG |
T9061 |
28626-28628 |
IN |
denotes |
of |
T9062 |
28629-28635 |
NN |
denotes |
TauEWS |
T9063 |
28643-28650 |
NN |
denotes |
Isl1Cre |
T9064 |
28635-28636 |
HYPH |
denotes |
- |
T9065 |
28636-28640 |
NN |
denotes |
Pea3 |
T9066 |
28640-28641 |
HYPH |
denotes |
/ |
T9067 |
28641-28642 |
SYM |
denotes |
+ |
T9068 |
28653-28660 |
NNS |
denotes |
embryos |
T9069 |
28650-28651 |
HYPH |
denotes |
/ |
T9070 |
28651-28652 |
SYM |
denotes |
+ |
T9071 |
28661-28662 |
-LRB- |
denotes |
( |
T9072 |
28670-28672 |
NN |
denotes |
7F |
T9073 |
28662-28669 |
NNS |
denotes |
Figures |
T9074 |
28673-28676 |
CC |
denotes |
and |
T9075 |
28677-28679 |
NN |
denotes |
S5 |
T9076 |
28679-28680 |
-RRB- |
denotes |
) |
T9077 |
28680-28681 |
. |
denotes |
. |
T9078 |
28681-28894 |
sentence |
denotes |
These findings suggest that DRG neurons in TauEWS-Pea3/+ Isl1Cre/+ embryos fail to differentiate to a normal fate and instead acquire an aberrant identity distinct from any subpopulation of wild-type DRG neurons. |
T9079 |
28682-28687 |
DT |
denotes |
These |
T9080 |
28688-28696 |
NNS |
denotes |
findings |
T9081 |
28697-28704 |
VBP |
denotes |
suggest |
T9082 |
28705-28709 |
IN |
denotes |
that |
T9083 |
28757-28761 |
VBP |
denotes |
fail |
T9084 |
28710-28713 |
NN |
denotes |
DRG |
T9085 |
28714-28721 |
NNS |
denotes |
neurons |
T9086 |
28722-28724 |
IN |
denotes |
in |
T9087 |
28725-28731 |
NN |
denotes |
TauEWS |
T9088 |
28739-28746 |
NN |
denotes |
Isl1Cre |
T9089 |
28731-28732 |
HYPH |
denotes |
- |
T9090 |
28732-28736 |
NN |
denotes |
Pea3 |
T9091 |
28736-28737 |
HYPH |
denotes |
/ |
T9092 |
28737-28738 |
SYM |
denotes |
+ |
T9093 |
28749-28756 |
NNS |
denotes |
embryos |
T9094 |
28746-28747 |
HYPH |
denotes |
/ |
T9095 |
28747-28748 |
SYM |
denotes |
+ |
T9096 |
28762-28764 |
TO |
denotes |
to |
T9097 |
28765-28778 |
VB |
denotes |
differentiate |
T9098 |
28779-28781 |
IN |
denotes |
to |
T9099 |
28782-28783 |
DT |
denotes |
a |
T9100 |
28791-28795 |
NN |
denotes |
fate |
T9101 |
28784-28790 |
JJ |
denotes |
normal |
T9102 |
28796-28799 |
CC |
denotes |
and |
T9103 |
28800-28807 |
RB |
denotes |
instead |
T9104 |
28808-28815 |
VB |
denotes |
acquire |
T9105 |
28816-28818 |
DT |
denotes |
an |
T9106 |
28828-28836 |
NN |
denotes |
identity |
T9107 |
28819-28827 |
JJ |
denotes |
aberrant |
T9108 |
28837-28845 |
JJ |
denotes |
distinct |
T9109 |
28846-28850 |
IN |
denotes |
from |
T9110 |
28851-28854 |
DT |
denotes |
any |
T9111 |
28855-28868 |
NN |
denotes |
subpopulation |
T9112 |
28869-28871 |
IN |
denotes |
of |
T9113 |
28872-28876 |
JJ |
denotes |
wild |
T9114 |
28877-28881 |
NN |
denotes |
type |
T9115 |
28876-28877 |
HYPH |
denotes |
- |
T9116 |
28886-28893 |
NNS |
denotes |
neurons |
T9117 |
28882-28885 |
NN |
denotes |
DRG |
T9118 |
28893-28894 |
. |
denotes |
. |
T9119 |
28894-29106 |
sentence |
denotes |
Finally, to assess whether EWS-Pea3 expressed precociously acts exclusively cell-autonomously or whether it may also influence neighboring DRG neurons, we activated expression of EWS-Pea3 using Hb9Cre mice [33]. |
T9120 |
28895-28902 |
RB |
denotes |
Finally |
T9121 |
29050-29059 |
VBD |
denotes |
activated |
T9122 |
28902-28904 |
, |
denotes |
, |
T9123 |
28904-28906 |
TO |
denotes |
to |
T9124 |
28907-28913 |
VB |
denotes |
assess |
T9125 |
28914-28921 |
IN |
denotes |
whether |
T9126 |
28954-28958 |
VBZ |
denotes |
acts |
T9127 |
28922-28925 |
NN |
denotes |
EWS |
T9128 |
28926-28930 |
NN |
denotes |
Pea3 |
T9129 |
28925-28926 |
HYPH |
denotes |
- |
T9130 |
28931-28940 |
VBN |
denotes |
expressed |
T9131 |
28941-28953 |
RB |
denotes |
precociously |
T9132 |
28959-28970 |
RB |
denotes |
exclusively |
T9133 |
28976-28988 |
RB |
denotes |
autonomously |
T9134 |
28971-28975 |
NN |
denotes |
cell |
T9135 |
28975-28976 |
HYPH |
denotes |
- |
T9136 |
28989-28991 |
CC |
denotes |
or |
T9137 |
28992-28999 |
IN |
denotes |
whether |
T9138 |
29012-29021 |
VB |
denotes |
influence |
T9139 |
29000-29002 |
PRP |
denotes |
it |
T9140 |
29003-29006 |
MD |
denotes |
may |
T9141 |
29007-29011 |
RB |
denotes |
also |
T9142 |
29022-29033 |
VBG |
denotes |
neighboring |
T9143 |
29038-29045 |
NNS |
denotes |
neurons |
T9144 |
29034-29037 |
NN |
denotes |
DRG |
T9145 |
29045-29047 |
, |
denotes |
, |
T9146 |
29047-29049 |
PRP |
denotes |
we |
T9147 |
29060-29070 |
NN |
denotes |
expression |
T9148 |
29071-29073 |
IN |
denotes |
of |
T9149 |
29074-29077 |
NN |
denotes |
EWS |
T9150 |
29078-29082 |
NN |
denotes |
Pea3 |
T9151 |
29077-29078 |
HYPH |
denotes |
- |
T9152 |
29083-29088 |
VBG |
denotes |
using |
T9153 |
29089-29095 |
NN |
denotes |
Hb9Cre |
T9154 |
29096-29100 |
NNS |
denotes |
mice |
T9155 |
29101-29102 |
-LRB- |
denotes |
[ |
T9156 |
29102-29104 |
CD |
denotes |
33 |
T9157 |
29104-29105 |
-RRB- |
denotes |
] |
T9158 |
29105-29106 |
. |
denotes |
. |
T9159 |
29106-29376 |
sentence |
denotes |
Due to a transient and rostro-caudally graded expression of Hb9 at neural plate stages, very few DRG neurons at brachial levels and increasingly more neurons progressing caudally undergo recombination in TauEWS-Pea3/+ Hb9Cre/+ and TaumGFP/+ Hb9Cre/+ embryos (Figure 8). |
T9160 |
29107-29110 |
IN |
denotes |
Due |
T9161 |
29286-29293 |
VBP |
denotes |
undergo |
T9162 |
29111-29113 |
IN |
denotes |
to |
T9163 |
29114-29115 |
DT |
denotes |
a |
T9164 |
29153-29163 |
NN |
denotes |
expression |
T9165 |
29116-29125 |
JJ |
denotes |
transient |
T9166 |
29126-29129 |
CC |
denotes |
and |
T9167 |
29130-29136 |
RB |
denotes |
rostro |
T9168 |
29137-29145 |
RB |
denotes |
caudally |
T9169 |
29136-29137 |
HYPH |
denotes |
- |
T9170 |
29146-29152 |
VBN |
denotes |
graded |
T9171 |
29164-29166 |
IN |
denotes |
of |
T9172 |
29167-29170 |
NN |
denotes |
Hb9 |
T9173 |
29171-29173 |
IN |
denotes |
at |
T9174 |
29174-29180 |
JJ |
denotes |
neural |
T9175 |
29181-29186 |
NN |
denotes |
plate |
T9176 |
29187-29193 |
NNS |
denotes |
stages |
T9177 |
29193-29195 |
, |
denotes |
, |
T9178 |
29195-29199 |
RB |
denotes |
very |
T9179 |
29200-29203 |
JJ |
denotes |
few |
T9180 |
29208-29215 |
NNS |
denotes |
neurons |
T9181 |
29204-29207 |
NN |
denotes |
DRG |
T9182 |
29216-29218 |
IN |
denotes |
at |
T9183 |
29219-29227 |
JJ |
denotes |
brachial |
T9184 |
29228-29234 |
NNS |
denotes |
levels |
T9185 |
29235-29238 |
CC |
denotes |
and |
T9186 |
29239-29251 |
RB |
denotes |
increasingly |
T9187 |
29252-29256 |
JJR |
denotes |
more |
T9188 |
29257-29264 |
NNS |
denotes |
neurons |
T9189 |
29265-29276 |
VBG |
denotes |
progressing |
T9190 |
29277-29285 |
RB |
denotes |
caudally |
T9191 |
29294-29307 |
NN |
denotes |
recombination |
T9192 |
29308-29310 |
IN |
denotes |
in |
T9193 |
29311-29317 |
NN |
denotes |
TauEWS |
T9194 |
29325-29331 |
NN |
denotes |
Hb9Cre |
T9195 |
29317-29318 |
HYPH |
denotes |
- |
T9196 |
29318-29322 |
NN |
denotes |
Pea3 |
T9197 |
29322-29323 |
HYPH |
denotes |
/ |
T9198 |
29323-29324 |
SYM |
denotes |
+ |
T9199 |
29357-29364 |
NNS |
denotes |
embryos |
T9200 |
29331-29332 |
HYPH |
denotes |
/ |
T9201 |
29332-29333 |
SYM |
denotes |
+ |
T9202 |
29334-29337 |
CC |
denotes |
and |
T9203 |
29338-29345 |
NN |
denotes |
TaumGFP |
T9204 |
29348-29354 |
NN |
denotes |
Hb9Cre |
T9205 |
29345-29346 |
HYPH |
denotes |
/ |
T9206 |
29346-29347 |
SYM |
denotes |
+ |
T9207 |
29354-29355 |
HYPH |
denotes |
/ |
T9208 |
29355-29356 |
SYM |
denotes |
+ |
T9209 |
29365-29366 |
-LRB- |
denotes |
( |
T9210 |
29366-29372 |
NN |
denotes |
Figure |
T9211 |
29373-29374 |
CD |
denotes |
8 |
T9212 |
29374-29375 |
-RRB- |
denotes |
) |
T9213 |
29375-29376 |
. |
denotes |
. |
T9214 |
29376-29598 |
sentence |
denotes |
Nevertheless, downregulation of Trk receptor expression or upregulation of Calretinin is restricted exclusively to neurons that have undergone recombination and cannot be observed in TaumGFP/+ Hb9Cre/+ embryos (Figure 8). |
T9215 |
29377-29389 |
RB |
denotes |
Nevertheless |
T9216 |
29466-29476 |
VBN |
denotes |
restricted |
T9217 |
29389-29391 |
, |
denotes |
, |
T9218 |
29391-29405 |
NN |
denotes |
downregulation |
T9219 |
29406-29408 |
IN |
denotes |
of |
T9220 |
29409-29412 |
NN |
denotes |
Trk |
T9221 |
29422-29432 |
NN |
denotes |
expression |
T9222 |
29413-29421 |
NN |
denotes |
receptor |
T9223 |
29433-29435 |
CC |
denotes |
or |
T9224 |
29436-29448 |
NN |
denotes |
upregulation |
T9225 |
29449-29451 |
IN |
denotes |
of |
T9226 |
29452-29462 |
NN |
denotes |
Calretinin |
T9227 |
29463-29465 |
VBZ |
denotes |
is |
T9228 |
29477-29488 |
RB |
denotes |
exclusively |
T9229 |
29489-29491 |
IN |
denotes |
to |
T9230 |
29492-29499 |
NNS |
denotes |
neurons |
T9231 |
29500-29504 |
WDT |
denotes |
that |
T9232 |
29510-29519 |
VBN |
denotes |
undergone |
T9233 |
29505-29509 |
VBP |
denotes |
have |
T9234 |
29520-29533 |
NN |
denotes |
recombination |
T9235 |
29534-29537 |
CC |
denotes |
and |
T9236 |
29538-29541 |
MD |
denotes |
can |
T9237 |
29548-29556 |
VBN |
denotes |
observed |
T9238 |
29541-29544 |
RB |
denotes |
not |
T9239 |
29545-29547 |
VB |
denotes |
be |
T9240 |
29557-29559 |
IN |
denotes |
in |
T9241 |
29560-29567 |
NN |
denotes |
TaumGFP |
T9242 |
29570-29576 |
NN |
denotes |
Hb9Cre |
T9243 |
29567-29568 |
HYPH |
denotes |
/ |
T9244 |
29568-29569 |
SYM |
denotes |
+ |
T9245 |
29579-29586 |
NNS |
denotes |
embryos |
T9246 |
29576-29577 |
HYPH |
denotes |
/ |
T9247 |
29577-29578 |
SYM |
denotes |
+ |
T9248 |
29587-29588 |
-LRB- |
denotes |
( |
T9249 |
29588-29594 |
NN |
denotes |
Figure |
T9250 |
29595-29596 |
CD |
denotes |
8 |
T9251 |
29596-29597 |
-RRB- |
denotes |
) |
T9252 |
29597-29598 |
. |
denotes |
. |
T9253 |
29598-29943 |
sentence |
denotes |
Together, these results and the findings obtained from in vitro culture experiments (see Figures 4 and 5) demonstrate that precocious or isochronic expression of EWS-Pea3 in the same neurons leads to significantly different cell-autonomous cellular responses with respect to gene expression, neuronal survival, and neurite outgrowth (Figure 9). |
T9254 |
29599-29607 |
RB |
denotes |
Together |
T9255 |
29705-29716 |
VBP |
denotes |
demonstrate |
T9256 |
29607-29609 |
, |
denotes |
, |
T9257 |
29609-29614 |
DT |
denotes |
these |
T9258 |
29615-29622 |
NNS |
denotes |
results |
T9259 |
29623-29626 |
CC |
denotes |
and |
T9260 |
29627-29630 |
DT |
denotes |
the |
T9261 |
29631-29639 |
NNS |
denotes |
findings |
T9262 |
29640-29648 |
VBN |
denotes |
obtained |
T9263 |
29649-29653 |
IN |
denotes |
from |
T9264 |
29654-29656 |
FW |
denotes |
in |
T9265 |
29657-29662 |
FW |
denotes |
vitro |
T9266 |
29671-29682 |
NNS |
denotes |
experiments |
T9267 |
29663-29670 |
NN |
denotes |
culture |
T9268 |
29683-29684 |
-LRB- |
denotes |
( |
T9269 |
29684-29687 |
VB |
denotes |
see |
T9270 |
29688-29695 |
NNS |
denotes |
Figures |
T9271 |
29696-29697 |
CD |
denotes |
4 |
T9272 |
29698-29701 |
CC |
denotes |
and |
T9273 |
29702-29703 |
CD |
denotes |
5 |
T9274 |
29703-29704 |
-RRB- |
denotes |
) |
T9275 |
29717-29721 |
IN |
denotes |
that |
T9276 |
29790-29795 |
VBZ |
denotes |
leads |
T9277 |
29722-29732 |
JJ |
denotes |
precocious |
T9278 |
29747-29757 |
NN |
denotes |
expression |
T9279 |
29733-29735 |
CC |
denotes |
or |
T9280 |
29736-29746 |
JJ |
denotes |
isochronic |
T9281 |
29758-29760 |
IN |
denotes |
of |
T9282 |
29761-29764 |
NN |
denotes |
EWS |
T9283 |
29765-29769 |
NN |
denotes |
Pea3 |
T9284 |
29764-29765 |
HYPH |
denotes |
- |
T9285 |
29770-29772 |
IN |
denotes |
in |
T9286 |
29773-29776 |
DT |
denotes |
the |
T9287 |
29782-29789 |
NNS |
denotes |
neurons |
T9288 |
29777-29781 |
JJ |
denotes |
same |
T9289 |
29796-29798 |
IN |
denotes |
to |
T9290 |
29799-29812 |
RB |
denotes |
significantly |
T9291 |
29813-29822 |
JJ |
denotes |
different |
T9292 |
29848-29857 |
NNS |
denotes |
responses |
T9293 |
29823-29827 |
NN |
denotes |
cell |
T9294 |
29828-29838 |
JJ |
denotes |
autonomous |
T9295 |
29827-29828 |
HYPH |
denotes |
- |
T9296 |
29839-29847 |
JJ |
denotes |
cellular |
T9297 |
29858-29862 |
IN |
denotes |
with |
T9298 |
29863-29870 |
NN |
denotes |
respect |
T9299 |
29871-29873 |
IN |
denotes |
to |
T9300 |
29874-29878 |
NN |
denotes |
gene |
T9301 |
29879-29889 |
NN |
denotes |
expression |
T9302 |
29889-29891 |
, |
denotes |
, |
T9303 |
29891-29899 |
JJ |
denotes |
neuronal |
T9304 |
29900-29908 |
NN |
denotes |
survival |
T9305 |
29908-29910 |
, |
denotes |
, |
T9306 |
29910-29913 |
CC |
denotes |
and |
T9307 |
29914-29921 |
NN |
denotes |
neurite |
T9308 |
29922-29931 |
NN |
denotes |
outgrowth |
T9309 |
29932-29933 |
-LRB- |
denotes |
( |
T9310 |
29933-29939 |
NN |
denotes |
Figure |
T9311 |
29940-29941 |
CD |
denotes |
9 |
T9312 |
29941-29942 |
-RRB- |
denotes |
) |
T9313 |
29942-29943 |
. |
denotes |
. |
T10766 |
32660-32666 |
NN |
denotes |
Target |
T10767 |
32667-32674 |
VBN |
denotes |
derived |
T10768 |
32666-32667 |
HYPH |
denotes |
- |
T10769 |
32675-32682 |
NNS |
denotes |
signals |
T10770 |
32683-32690 |
VBP |
denotes |
exhibit |
T10771 |
32691-32692 |
DT |
denotes |
a |
T10772 |
32703-32707 |
NN |
denotes |
role |
T10773 |
32693-32702 |
VBN |
denotes |
conserved |
T10774 |
32708-32710 |
IN |
denotes |
in |
T10775 |
32711-32714 |
DT |
denotes |
the |
T10776 |
32715-32724 |
NN |
denotes |
induction |
T10777 |
32725-32727 |
IN |
denotes |
of |
T10778 |
32728-32735 |
VBN |
denotes |
defined |
T10779 |
32736-32744 |
NNS |
denotes |
programs |
T10780 |
32745-32747 |
IN |
denotes |
of |
T10781 |
32748-32761 |
NN |
denotes |
transcription |
T10782 |
32762-32768 |
NN |
denotes |
factor |
T10783 |
32769-32779 |
NN |
denotes |
expression |
T10784 |
32780-32784 |
JJ |
denotes |
late |
T10785 |
32785-32787 |
IN |
denotes |
in |
T10786 |
32788-32800 |
JJ |
denotes |
post-mitotic |
T10787 |
32810-32825 |
NN |
denotes |
differentiation |
T10788 |
32801-32809 |
JJ |
denotes |
neuronal |
T10789 |
32826-32827 |
-LRB- |
denotes |
[ |
T10790 |
32827-32829 |
CD |
denotes |
10 |
T10791 |
32829-32830 |
-RRB- |
denotes |
] |
T10792 |
32830-32831 |
. |
denotes |
. |
T10793 |
32831-33002 |
sentence |
denotes |
This study provides evidence that the late onset of transcription factor expression is essential for many later aspects of neuronal differentiation and circuit formation. |
T10794 |
32832-32836 |
DT |
denotes |
This |
T10795 |
32837-32842 |
NN |
denotes |
study |
T10796 |
32843-32851 |
VBZ |
denotes |
provides |
T10797 |
32852-32860 |
NN |
denotes |
evidence |
T10798 |
32861-32865 |
IN |
denotes |
that |
T10799 |
32916-32918 |
VBZ |
denotes |
is |
T10800 |
32866-32869 |
DT |
denotes |
the |
T10801 |
32875-32880 |
NN |
denotes |
onset |
T10802 |
32870-32874 |
JJ |
denotes |
late |
T10803 |
32881-32883 |
IN |
denotes |
of |
T10804 |
32884-32897 |
NN |
denotes |
transcription |
T10805 |
32898-32904 |
NN |
denotes |
factor |
T10806 |
32905-32915 |
NN |
denotes |
expression |
T10807 |
32919-32928 |
JJ |
denotes |
essential |
T10808 |
32929-32932 |
IN |
denotes |
for |
T10809 |
32933-32937 |
JJ |
denotes |
many |
T10810 |
32944-32951 |
NNS |
denotes |
aspects |
T10811 |
32938-32943 |
JJ |
denotes |
later |
T10812 |
32952-32954 |
IN |
denotes |
of |
T10813 |
32955-32963 |
JJ |
denotes |
neuronal |
T10814 |
32964-32979 |
NN |
denotes |
differentiation |
T10815 |
32980-32983 |
CC |
denotes |
and |
T10816 |
32984-32991 |
NN |
denotes |
circuit |
T10817 |
32992-33001 |
NN |
denotes |
formation |
T10818 |
33001-33002 |
. |
denotes |
. |
T10819 |
33002-33214 |
sentence |
denotes |
Our data indicate that DRG neurons undergo a temporal change in their competence to respond to ETS transcription factor signaling, as assessed by changes in gene expression and axonal target invasion (Figure 9). |
T10820 |
33003-33006 |
PRP$ |
denotes |
Our |
T10821 |
33007-33011 |
NNS |
denotes |
data |
T10822 |
33012-33020 |
VBP |
denotes |
indicate |
T10823 |
33021-33025 |
IN |
denotes |
that |
T10824 |
33038-33045 |
VBP |
denotes |
undergo |
T10825 |
33026-33029 |
NN |
denotes |
DRG |
T10826 |
33030-33037 |
NNS |
denotes |
neurons |
T10827 |
33046-33047 |
DT |
denotes |
a |
T10828 |
33057-33063 |
NN |
denotes |
change |
T10829 |
33048-33056 |
JJ |
denotes |
temporal |
T10830 |
33064-33066 |
IN |
denotes |
in |
T10831 |
33067-33072 |
PRP$ |
denotes |
their |
T10832 |
33073-33083 |
NN |
denotes |
competence |
T10833 |
33084-33086 |
TO |
denotes |
to |
T10834 |
33087-33094 |
VB |
denotes |
respond |
T10835 |
33095-33097 |
IN |
denotes |
to |
T10836 |
33098-33101 |
NN |
denotes |
ETS |
T10837 |
33123-33132 |
NN |
denotes |
signaling |
T10838 |
33102-33115 |
NN |
denotes |
transcription |
T10839 |
33116-33122 |
NN |
denotes |
factor |
T10840 |
33132-33134 |
, |
denotes |
, |
T10841 |
33134-33136 |
IN |
denotes |
as |
T10842 |
33137-33145 |
VBN |
denotes |
assessed |
T10843 |
33146-33148 |
IN |
denotes |
by |
T10844 |
33149-33156 |
NNS |
denotes |
changes |
T10845 |
33157-33159 |
IN |
denotes |
in |
T10846 |
33160-33164 |
NN |
denotes |
gene |
T10847 |
33165-33175 |
NN |
denotes |
expression |
T10848 |
33176-33179 |
CC |
denotes |
and |
T10849 |
33180-33186 |
JJ |
denotes |
axonal |
T10850 |
33194-33202 |
NN |
denotes |
invasion |
T10851 |
33187-33193 |
NN |
denotes |
target |
T10852 |
33203-33204 |
-LRB- |
denotes |
( |
T10853 |
33204-33210 |
NN |
denotes |
Figure |
T10854 |
33211-33212 |
CD |
denotes |
9 |
T10855 |
33212-33213 |
-RRB- |
denotes |
) |
T10856 |
33213-33214 |
. |
denotes |
. |
T10857 |
33214-33359 |
sentence |
denotes |
Our findings argue for the necessity of target-induced, and therefore temporally controlled, upregulation of ETS transcription factor signaling. |
T10858 |
33215-33218 |
PRP$ |
denotes |
Our |
T10859 |
33219-33227 |
NNS |
denotes |
findings |
T10860 |
33228-33233 |
VBP |
denotes |
argue |
T10861 |
33234-33237 |
IN |
denotes |
for |
T10862 |
33238-33241 |
DT |
denotes |
the |
T10863 |
33242-33251 |
NN |
denotes |
necessity |
T10864 |
33252-33254 |
IN |
denotes |
of |
T10865 |
33255-33261 |
NN |
denotes |
target |
T10866 |
33262-33269 |
VBN |
denotes |
induced |
T10867 |
33261-33262 |
HYPH |
denotes |
- |
T10868 |
33308-33320 |
NN |
denotes |
upregulation |
T10869 |
33269-33271 |
, |
denotes |
, |
T10870 |
33271-33274 |
CC |
denotes |
and |
T10871 |
33275-33284 |
RB |
denotes |
therefore |
T10872 |
33285-33295 |
RB |
denotes |
temporally |
T10873 |
33296-33306 |
VBN |
denotes |
controlled |
T10874 |
33306-33308 |
, |
denotes |
, |
T10875 |
33321-33323 |
IN |
denotes |
of |
T10876 |
33324-33327 |
NN |
denotes |
ETS |
T10877 |
33349-33358 |
NN |
denotes |
signaling |
T10878 |
33328-33341 |
NN |
denotes |
transcription |
T10879 |
33342-33348 |
NN |
denotes |
factor |
T10880 |
33358-33359 |
. |
denotes |
. |
T10881 |
33359-33589 |
sentence |
denotes |
More generally, they suggest that temporally regulated activation of transcriptional programs coupled to a particular fate induced in neurons at early developmental stages represents an important mechanism of neuronal maturation. |
T10882 |
33360-33364 |
RBR |
denotes |
More |
T10883 |
33365-33374 |
RB |
denotes |
generally |
T10884 |
33381-33388 |
VBP |
denotes |
suggest |
T10885 |
33374-33376 |
, |
denotes |
, |
T10886 |
33376-33380 |
PRP |
denotes |
they |
T10887 |
33389-33393 |
IN |
denotes |
that |
T10888 |
33532-33542 |
VBZ |
denotes |
represents |
T10889 |
33394-33404 |
RB |
denotes |
temporally |
T10890 |
33405-33414 |
VBN |
denotes |
regulated |
T10891 |
33415-33425 |
NN |
denotes |
activation |
T10892 |
33426-33428 |
IN |
denotes |
of |
T10893 |
33429-33444 |
JJ |
denotes |
transcriptional |
T10894 |
33445-33453 |
NNS |
denotes |
programs |
T10895 |
33454-33461 |
VBN |
denotes |
coupled |
T10896 |
33462-33464 |
IN |
denotes |
to |
T10897 |
33465-33466 |
DT |
denotes |
a |
T10898 |
33478-33482 |
NN |
denotes |
fate |
T10899 |
33467-33477 |
JJ |
denotes |
particular |
T10900 |
33483-33490 |
VBN |
denotes |
induced |
T10901 |
33491-33493 |
IN |
denotes |
in |
T10902 |
33494-33501 |
NNS |
denotes |
neurons |
T10903 |
33502-33504 |
IN |
denotes |
at |
T10904 |
33505-33510 |
JJ |
denotes |
early |
T10905 |
33525-33531 |
NNS |
denotes |
stages |
T10906 |
33511-33524 |
JJ |
denotes |
developmental |
T10907 |
33543-33545 |
DT |
denotes |
an |
T10908 |
33556-33565 |
NN |
denotes |
mechanism |
T10909 |
33546-33555 |
JJ |
denotes |
important |
T10910 |
33566-33568 |
IN |
denotes |
of |
T10911 |
33569-33577 |
JJ |
denotes |
neuronal |
T10912 |
33578-33588 |
NN |
denotes |
maturation |
T10913 |
33588-33589 |
. |
denotes |
. |
T10914 |
33589-33800 |
sentence |
denotes |
One striking observation of this study is that precocious induction of ETS signaling promotes neuronal survival without a requirement for neurotrophic support and in complete absence of Trk receptor expression. |
T10915 |
33590-33593 |
CD |
denotes |
One |
T10916 |
33603-33614 |
NN |
denotes |
observation |
T10917 |
33594-33602 |
JJ |
denotes |
striking |
T10918 |
33629-33631 |
VBZ |
denotes |
is |
T10919 |
33615-33617 |
IN |
denotes |
of |
T10920 |
33618-33622 |
DT |
denotes |
this |
T10921 |
33623-33628 |
NN |
denotes |
study |
T10922 |
33632-33636 |
IN |
denotes |
that |
T10923 |
33675-33683 |
VBZ |
denotes |
promotes |
T10924 |
33637-33647 |
JJ |
denotes |
precocious |
T10925 |
33648-33657 |
NN |
denotes |
induction |
T10926 |
33658-33660 |
IN |
denotes |
of |
T10927 |
33661-33664 |
NN |
denotes |
ETS |
T10928 |
33665-33674 |
NN |
denotes |
signaling |
T10929 |
33684-33692 |
JJ |
denotes |
neuronal |
T10930 |
33693-33701 |
NN |
denotes |
survival |
T10931 |
33702-33709 |
IN |
denotes |
without |
T10932 |
33710-33711 |
DT |
denotes |
a |
T10933 |
33712-33723 |
NN |
denotes |
requirement |
T10934 |
33724-33727 |
IN |
denotes |
for |
T10935 |
33728-33740 |
JJ |
denotes |
neurotrophic |
T10936 |
33741-33748 |
NN |
denotes |
support |
T10937 |
33749-33752 |
CC |
denotes |
and |
T10938 |
33753-33755 |
IN |
denotes |
in |
T10939 |
33756-33764 |
JJ |
denotes |
complete |
T10940 |
33765-33772 |
NN |
denotes |
absence |
T10941 |
33773-33775 |
IN |
denotes |
of |
T10942 |
33776-33779 |
NN |
denotes |
Trk |
T10943 |
33780-33788 |
NN |
denotes |
receptor |
T10944 |
33789-33799 |
NN |
denotes |
expression |
T10945 |
33799-33800 |
. |
denotes |
. |
T10946 |
33800-34041 |
sentence |
denotes |
In contrast, ETS signaling at the normal time of onset of Er81 expression does not result in enhanced neuronal survival in the absence of neurotrophins and also does not lead to downregulation of TrkC expression in proprioceptive afferents. |
T10947 |
33801-33803 |
IN |
denotes |
In |
T10948 |
33884-33890 |
VB |
denotes |
result |
T10949 |
33804-33812 |
NN |
denotes |
contrast |
T10950 |
33812-33814 |
, |
denotes |
, |
T10951 |
33814-33817 |
NN |
denotes |
ETS |
T10952 |
33818-33827 |
NN |
denotes |
signaling |
T10953 |
33828-33830 |
IN |
denotes |
at |
T10954 |
33831-33834 |
DT |
denotes |
the |
T10955 |
33842-33846 |
NN |
denotes |
time |
T10956 |
33835-33841 |
JJ |
denotes |
normal |
T10957 |
33847-33849 |
IN |
denotes |
of |
T10958 |
33850-33855 |
NN |
denotes |
onset |
T10959 |
33856-33858 |
IN |
denotes |
of |
T10960 |
33859-33863 |
NN |
denotes |
Er81 |
T10961 |
33864-33874 |
NN |
denotes |
expression |
T10962 |
33875-33879 |
VBZ |
denotes |
does |
T10963 |
33880-33883 |
RB |
denotes |
not |
T10964 |
33891-33893 |
IN |
denotes |
in |
T10965 |
33894-33902 |
VBN |
denotes |
enhanced |
T10966 |
33912-33920 |
NN |
denotes |
survival |
T10967 |
33903-33911 |
JJ |
denotes |
neuronal |
T10968 |
33921-33923 |
IN |
denotes |
in |
T10969 |
33924-33927 |
DT |
denotes |
the |
T10970 |
33928-33935 |
NN |
denotes |
absence |
T10971 |
33936-33938 |
IN |
denotes |
of |
T10972 |
33939-33952 |
NNS |
denotes |
neurotrophins |
T10973 |
33953-33956 |
CC |
denotes |
and |
T10974 |
33957-33961 |
RB |
denotes |
also |
T10975 |
33971-33975 |
VB |
denotes |
lead |
T10976 |
33962-33966 |
VBZ |
denotes |
does |
T10977 |
33967-33970 |
RB |
denotes |
not |
T10978 |
33976-33978 |
IN |
denotes |
to |
T10979 |
33979-33993 |
NN |
denotes |
downregulation |
T10980 |
33994-33996 |
IN |
denotes |
of |
T10981 |
33997-34001 |
NN |
denotes |
TrkC |
T10982 |
34002-34012 |
NN |
denotes |
expression |
T10983 |
34013-34015 |
IN |
denotes |
in |
T10984 |
34016-34030 |
JJ |
denotes |
proprioceptive |
T10985 |
34031-34040 |
NNS |
denotes |
afferents |
T10986 |
34040-34041 |
. |
denotes |
. |
T10987 |
34041-34209 |
sentence |
denotes |
These findings demonstrate very distinct activities of one transcriptional regulator at different developmental steps within a committed post-mitotic neuronal lineage. |
T10988 |
34042-34047 |
DT |
denotes |
These |
T10989 |
34048-34056 |
NNS |
denotes |
findings |
T10990 |
34057-34068 |
VBP |
denotes |
demonstrate |
T10991 |
34069-34073 |
RB |
denotes |
very |
T10992 |
34074-34082 |
JJ |
denotes |
distinct |
T10993 |
34083-34093 |
NNS |
denotes |
activities |
T10994 |
34094-34096 |
IN |
denotes |
of |
T10995 |
34097-34100 |
CD |
denotes |
one |
T10996 |
34117-34126 |
NN |
denotes |
regulator |
T10997 |
34101-34116 |
JJ |
denotes |
transcriptional |
T10998 |
34127-34129 |
IN |
denotes |
at |
T10999 |
34130-34139 |
JJ |
denotes |
different |
T11000 |
34154-34159 |
NNS |
denotes |
steps |
T11001 |
34140-34153 |
JJ |
denotes |
developmental |
T11002 |
34160-34166 |
IN |
denotes |
within |
T11003 |
34167-34168 |
DT |
denotes |
a |
T11004 |
34201-34208 |
NN |
denotes |
lineage |
T11005 |
34169-34178 |
VBN |
denotes |
committed |
T11006 |
34179-34191 |
JJ |
denotes |
post-mitotic |
T11007 |
34192-34200 |
JJ |
denotes |
neuronal |
T11008 |
34208-34209 |
. |
denotes |
. |
T11009 |
34209-34364 |
sentence |
denotes |
The absence of Trk receptor expression upon precocious induction of ETS signaling can only partially explain the observed phenotype in axonal projections. |
T11010 |
34210-34213 |
DT |
denotes |
The |
T11011 |
34214-34221 |
NN |
denotes |
absence |
T11012 |
34311-34318 |
VB |
denotes |
explain |
T11013 |
34222-34224 |
IN |
denotes |
of |
T11014 |
34225-34228 |
NN |
denotes |
Trk |
T11015 |
34238-34248 |
NN |
denotes |
expression |
T11016 |
34229-34237 |
NN |
denotes |
receptor |
T11017 |
34249-34253 |
IN |
denotes |
upon |
T11018 |
34254-34264 |
JJ |
denotes |
precocious |
T11019 |
34265-34274 |
NN |
denotes |
induction |
T11020 |
34275-34277 |
IN |
denotes |
of |
T11021 |
34278-34281 |
NN |
denotes |
ETS |
T11022 |
34282-34291 |
NN |
denotes |
signaling |
T11023 |
34292-34295 |
MD |
denotes |
can |
T11024 |
34296-34300 |
RB |
denotes |
only |
T11025 |
34301-34310 |
RB |
denotes |
partially |
T11026 |
34319-34322 |
DT |
denotes |
the |
T11027 |
34332-34341 |
NN |
denotes |
phenotype |
T11028 |
34323-34331 |
VBN |
denotes |
observed |
T11029 |
34342-34344 |
IN |
denotes |
in |
T11030 |
34345-34351 |
JJ |
denotes |
axonal |
T11031 |
34352-34363 |
NNS |
denotes |
projections |
T11032 |
34363-34364 |
. |
denotes |
. |
T11033 |
34364-34576 |
sentence |
denotes |
Elimination of TrkA receptor signaling in Bax mutant mice perturbs establishment of peripheral projections of cutaneous afferents, whereas establishment of central projections does not appear to be affected [8]. |
T11034 |
34365-34376 |
NN |
denotes |
Elimination |
T11035 |
34423-34431 |
VBZ |
denotes |
perturbs |
T11036 |
34377-34379 |
IN |
denotes |
of |
T11037 |
34380-34384 |
NN |
denotes |
TrkA |
T11038 |
34385-34393 |
NN |
denotes |
receptor |
T11039 |
34394-34403 |
NN |
denotes |
signaling |
T11040 |
34404-34406 |
IN |
denotes |
in |
T11041 |
34407-34410 |
NN |
denotes |
Bax |
T11042 |
34418-34422 |
NNS |
denotes |
mice |
T11043 |
34411-34417 |
NN |
denotes |
mutant |
T11044 |
34432-34445 |
NN |
denotes |
establishment |
T11045 |
34446-34448 |
IN |
denotes |
of |
T11046 |
34449-34459 |
JJ |
denotes |
peripheral |
T11047 |
34460-34471 |
NNS |
denotes |
projections |
T11048 |
34472-34474 |
IN |
denotes |
of |
T11049 |
34475-34484 |
JJ |
denotes |
cutaneous |
T11050 |
34485-34494 |
NNS |
denotes |
afferents |
T11051 |
34494-34496 |
, |
denotes |
, |
T11052 |
34496-34503 |
IN |
denotes |
whereas |
T11053 |
34550-34556 |
VB |
denotes |
appear |
T11054 |
34504-34517 |
NN |
denotes |
establishment |
T11055 |
34518-34520 |
IN |
denotes |
of |
T11056 |
34521-34528 |
JJ |
denotes |
central |
T11057 |
34529-34540 |
NNS |
denotes |
projections |
T11058 |
34541-34545 |
VBZ |
denotes |
does |
T11059 |
34546-34549 |
RB |
denotes |
not |
T11060 |
34557-34559 |
TO |
denotes |
to |
T11061 |
34563-34571 |
VBN |
denotes |
affected |
T11062 |
34560-34562 |
VB |
denotes |
be |
T11063 |
34572-34573 |
-LRB- |
denotes |
[ |
T11064 |
34573-34574 |
CD |
denotes |
8 |
T11065 |
34574-34575 |
-RRB- |
denotes |
] |
T11066 |
34575-34576 |
. |
denotes |
. |
T11067 |
34576-34709 |
sentence |
denotes |
In the absence of NT-3 signaling, development of central as well as peripheral proprioceptive afferent projections is perturbed [9]. |
T11068 |
34577-34579 |
IN |
denotes |
In |
T11069 |
34695-34704 |
VBN |
denotes |
perturbed |
T11070 |
34580-34583 |
DT |
denotes |
the |
T11071 |
34584-34591 |
NN |
denotes |
absence |
T11072 |
34592-34594 |
IN |
denotes |
of |
T11073 |
34595-34597 |
NN |
denotes |
NT |
T11074 |
34600-34609 |
NN |
denotes |
signaling |
T11075 |
34597-34598 |
HYPH |
denotes |
- |
T11076 |
34598-34599 |
CD |
denotes |
3 |
T11077 |
34609-34611 |
, |
denotes |
, |
T11078 |
34611-34622 |
NN |
denotes |
development |
T11079 |
34623-34625 |
IN |
denotes |
of |
T11080 |
34626-34633 |
JJ |
denotes |
central |
T11081 |
34680-34691 |
NNS |
denotes |
projections |
T11082 |
34634-34636 |
RB |
denotes |
as |
T11083 |
34642-34644 |
IN |
denotes |
as |
T11084 |
34637-34641 |
RB |
denotes |
well |
T11085 |
34645-34655 |
JJ |
denotes |
peripheral |
T11086 |
34656-34670 |
JJ |
denotes |
proprioceptive |
T11087 |
34671-34679 |
JJ |
denotes |
afferent |
T11088 |
34692-34694 |
VBZ |
denotes |
is |
T11089 |
34705-34706 |
-LRB- |
denotes |
[ |
T11090 |
34706-34707 |
CD |
denotes |
9 |
T11091 |
34707-34708 |
-RRB- |
denotes |
] |
T11092 |
34708-34709 |
. |
denotes |
. |
T11093 |
34709-34887 |
sentence |
denotes |
In contrast, upon precocious induction of ETS signaling, we found more pronounced defects in the establishment of central rather than peripheral projections for all DRG neurons. |
T11094 |
34710-34712 |
IN |
denotes |
In |
T11095 |
34770-34775 |
VBD |
denotes |
found |
T11096 |
34713-34721 |
NN |
denotes |
contrast |
T11097 |
34721-34723 |
, |
denotes |
, |
T11098 |
34723-34727 |
IN |
denotes |
upon |
T11099 |
34728-34738 |
JJ |
denotes |
precocious |
T11100 |
34739-34748 |
NN |
denotes |
induction |
T11101 |
34749-34751 |
IN |
denotes |
of |
T11102 |
34752-34755 |
NN |
denotes |
ETS |
T11103 |
34756-34765 |
NN |
denotes |
signaling |
T11104 |
34765-34767 |
, |
denotes |
, |
T11105 |
34767-34769 |
PRP |
denotes |
we |
T11106 |
34776-34780 |
RBR |
denotes |
more |
T11107 |
34781-34791 |
JJ |
denotes |
pronounced |
T11108 |
34792-34799 |
NNS |
denotes |
defects |
T11109 |
34800-34802 |
IN |
denotes |
in |
T11110 |
34803-34806 |
DT |
denotes |
the |
T11111 |
34807-34820 |
NN |
denotes |
establishment |
T11112 |
34821-34823 |
IN |
denotes |
of |
T11113 |
34824-34831 |
JJ |
denotes |
central |
T11114 |
34855-34866 |
NNS |
denotes |
projections |
T11115 |
34832-34838 |
RB |
denotes |
rather |
T11116 |
34839-34843 |
IN |
denotes |
than |
T11117 |
34844-34854 |
JJ |
denotes |
peripheral |
T11118 |
34867-34870 |
IN |
denotes |
for |
T11119 |
34871-34874 |
DT |
denotes |
all |
T11120 |
34879-34886 |
NNS |
denotes |
neurons |
T11121 |
34875-34878 |
NN |
denotes |
DRG |
T11122 |
34886-34887 |
. |
denotes |
. |
T11123 |
34887-35120 |
sentence |
denotes |
Induction of Er81 expression in proprioceptive afferents is controlled by peripheral NT-3 as axons reach the vicinity of target muscles, and thus occurs only approximately 3 d after proprioceptive neurons become post-mitotic [9,14]. |
T11124 |
34888-34897 |
NN |
denotes |
Induction |
T11125 |
34948-34958 |
VBN |
denotes |
controlled |
T11126 |
34898-34900 |
IN |
denotes |
of |
T11127 |
34901-34905 |
NN |
denotes |
Er81 |
T11128 |
34906-34916 |
NN |
denotes |
expression |
T11129 |
34917-34919 |
IN |
denotes |
in |
T11130 |
34920-34934 |
JJ |
denotes |
proprioceptive |
T11131 |
34935-34944 |
NNS |
denotes |
afferents |
T11132 |
34945-34947 |
VBZ |
denotes |
is |
T11133 |
34959-34961 |
IN |
denotes |
by |
T11134 |
34962-34972 |
JJ |
denotes |
peripheral |
T11135 |
34973-34975 |
NN |
denotes |
NT |
T11136 |
34975-34976 |
HYPH |
denotes |
- |
T11137 |
34976-34977 |
CD |
denotes |
3 |
T11138 |
34978-34980 |
IN |
denotes |
as |
T11139 |
34987-34992 |
VBP |
denotes |
reach |
T11140 |
34981-34986 |
NNS |
denotes |
axons |
T11141 |
34993-34996 |
DT |
denotes |
the |
T11142 |
34997-35005 |
NN |
denotes |
vicinity |
T11143 |
35006-35008 |
IN |
denotes |
of |
T11144 |
35009-35015 |
NN |
denotes |
target |
T11145 |
35016-35023 |
NNS |
denotes |
muscles |
T11146 |
35023-35025 |
, |
denotes |
, |
T11147 |
35025-35028 |
CC |
denotes |
and |
T11148 |
35029-35033 |
RB |
denotes |
thus |
T11149 |
35034-35040 |
VBZ |
denotes |
occurs |
T11150 |
35041-35045 |
RB |
denotes |
only |
T11151 |
35062-35063 |
NN |
denotes |
d |
T11152 |
35046-35059 |
RB |
denotes |
approximately |
T11153 |
35060-35061 |
CD |
denotes |
3 |
T11154 |
35093-35099 |
VBP |
denotes |
become |
T11155 |
35064-35069 |
IN |
denotes |
after |
T11156 |
35070-35084 |
JJ |
denotes |
proprioceptive |
T11157 |
35085-35092 |
NNS |
denotes |
neurons |
T11158 |
35100-35112 |
JJ |
denotes |
post-mitotic |
T11159 |
35113-35114 |
-LRB- |
denotes |
[ |
T11160 |
35116-35118 |
CD |
denotes |
14 |
T11161 |
35114-35115 |
CD |
denotes |
9 |
T11162 |
35115-35116 |
, |
denotes |
, |
T11163 |
35118-35119 |
-RRB- |
denotes |
] |
T11164 |
35119-35120 |
. |
denotes |
. |
T11165 |
35120-35378 |
sentence |
denotes |
This temporally delayed and target-induced upregulation of ETS transcription factor expression several days after a neuronal lineage of a specific identity first emerges is not restricted to DRG sensory neurons, but is also found in motor neuron pools [13]. |
T11166 |
35121-35125 |
DT |
denotes |
This |
T11167 |
35164-35176 |
NN |
denotes |
upregulation |
T11168 |
35126-35136 |
RB |
denotes |
temporally |
T11169 |
35137-35144 |
VBN |
denotes |
delayed |
T11170 |
35145-35148 |
CC |
denotes |
and |
T11171 |
35149-35155 |
NN |
denotes |
target |
T11172 |
35156-35163 |
VBN |
denotes |
induced |
T11173 |
35155-35156 |
HYPH |
denotes |
- |
T11174 |
35298-35308 |
VBN |
denotes |
restricted |
T11175 |
35177-35179 |
IN |
denotes |
of |
T11176 |
35180-35183 |
NN |
denotes |
ETS |
T11177 |
35205-35215 |
NN |
denotes |
expression |
T11178 |
35184-35197 |
NN |
denotes |
transcription |
T11179 |
35198-35204 |
NN |
denotes |
factor |
T11180 |
35216-35223 |
JJ |
denotes |
several |
T11181 |
35224-35228 |
NNS |
denotes |
days |
T11182 |
35283-35290 |
VBZ |
denotes |
emerges |
T11183 |
35229-35234 |
IN |
denotes |
after |
T11184 |
35235-35236 |
DT |
denotes |
a |
T11185 |
35246-35253 |
NN |
denotes |
lineage |
T11186 |
35237-35245 |
JJ |
denotes |
neuronal |
T11187 |
35254-35256 |
IN |
denotes |
of |
T11188 |
35257-35258 |
DT |
denotes |
a |
T11189 |
35268-35276 |
NN |
denotes |
identity |
T11190 |
35259-35267 |
JJ |
denotes |
specific |
T11191 |
35277-35282 |
RB |
denotes |
first |
T11192 |
35291-35293 |
VBZ |
denotes |
is |
T11193 |
35294-35297 |
RB |
denotes |
not |
T11194 |
35309-35311 |
IN |
denotes |
to |
T11195 |
35312-35315 |
NN |
denotes |
DRG |
T11196 |
35324-35331 |
NNS |
denotes |
neurons |
T11197 |
35316-35323 |
JJ |
denotes |
sensory |
T11198 |
35331-35333 |
, |
denotes |
, |
T11199 |
35333-35336 |
CC |
denotes |
but |
T11200 |
35337-35339 |
VBZ |
denotes |
is |
T11201 |
35345-35350 |
VBN |
denotes |
found |
T11202 |
35340-35344 |
RB |
denotes |
also |
T11203 |
35351-35353 |
IN |
denotes |
in |
T11204 |
35354-35359 |
NN |
denotes |
motor |
T11205 |
35360-35366 |
NN |
denotes |
neuron |
T11206 |
35367-35372 |
NNS |
denotes |
pools |
T11207 |
35373-35374 |
-LRB- |
denotes |
[ |
T11208 |
35374-35376 |
CD |
denotes |
13 |
T11209 |
35376-35377 |
-RRB- |
denotes |
] |
T11210 |
35377-35378 |
. |
denotes |
. |
T11211 |
35378-35765 |
sentence |
denotes |
Target-derived factors have also been implicated in controlling neuronal maturation of predetermined neurons in Drosophila, in which expression of members of the BMP family in the target region is essential for the induction of mature peptidergic properties in a subpopulation of neurons marked by the coordinate expression of the two transcription factors Apterous and Squeeze [11,12]. |
T11212 |
35379-35385 |
NN |
denotes |
Target |
T11213 |
35386-35393 |
VBN |
denotes |
derived |
T11214 |
35385-35386 |
HYPH |
denotes |
- |
T11215 |
35394-35401 |
NNS |
denotes |
factors |
T11216 |
35417-35427 |
VBN |
denotes |
implicated |
T11217 |
35402-35406 |
VBP |
denotes |
have |
T11218 |
35407-35411 |
RB |
denotes |
also |
T11219 |
35412-35416 |
VBN |
denotes |
been |
T11220 |
35428-35430 |
IN |
denotes |
in |
T11221 |
35431-35442 |
VBG |
denotes |
controlling |
T11222 |
35443-35451 |
JJ |
denotes |
neuronal |
T11223 |
35452-35462 |
NN |
denotes |
maturation |
T11224 |
35463-35465 |
IN |
denotes |
of |
T11225 |
35466-35479 |
VBN |
denotes |
predetermined |
T11226 |
35480-35487 |
NNS |
denotes |
neurons |
T11227 |
35488-35490 |
IN |
denotes |
in |
T11228 |
35491-35501 |
NNP |
denotes |
Drosophila |
T11229 |
35501-35503 |
, |
denotes |
, |
T11230 |
35503-35505 |
IN |
denotes |
in |
T11231 |
35573-35575 |
VBZ |
denotes |
is |
T11232 |
35506-35511 |
WDT |
denotes |
which |
T11233 |
35512-35522 |
NN |
denotes |
expression |
T11234 |
35523-35525 |
IN |
denotes |
of |
T11235 |
35526-35533 |
NNS |
denotes |
members |
T11236 |
35534-35536 |
IN |
denotes |
of |
T11237 |
35537-35540 |
DT |
denotes |
the |
T11238 |
35545-35551 |
NN |
denotes |
family |
T11239 |
35541-35544 |
NN |
denotes |
BMP |
T11240 |
35552-35554 |
IN |
denotes |
in |
T11241 |
35555-35558 |
DT |
denotes |
the |
T11242 |
35566-35572 |
NN |
denotes |
region |
T11243 |
35559-35565 |
NN |
denotes |
target |
T11244 |
35576-35585 |
JJ |
denotes |
essential |
T11245 |
35586-35589 |
IN |
denotes |
for |
T11246 |
35590-35593 |
DT |
denotes |
the |
T11247 |
35594-35603 |
NN |
denotes |
induction |
T11248 |
35604-35606 |
IN |
denotes |
of |
T11249 |
35607-35613 |
JJ |
denotes |
mature |
T11250 |
35626-35636 |
NNS |
denotes |
properties |
T11251 |
35614-35625 |
JJ |
denotes |
peptidergic |
T11252 |
35637-35639 |
IN |
denotes |
in |
T11253 |
35640-35641 |
DT |
denotes |
a |
T11254 |
35642-35655 |
NN |
denotes |
subpopulation |
T11255 |
35656-35658 |
IN |
denotes |
of |
T11256 |
35659-35666 |
NNS |
denotes |
neurons |
T11257 |
35667-35673 |
VBN |
denotes |
marked |
T11258 |
35674-35676 |
IN |
denotes |
by |
T11259 |
35677-35680 |
DT |
denotes |
the |
T11260 |
35692-35702 |
NN |
denotes |
expression |
T11261 |
35681-35691 |
NN |
denotes |
coordinate |
T11262 |
35703-35705 |
IN |
denotes |
of |
T11263 |
35706-35709 |
DT |
denotes |
the |
T11264 |
35728-35735 |
NNS |
denotes |
factors |
T11265 |
35710-35713 |
CD |
denotes |
two |
T11266 |
35714-35727 |
NN |
denotes |
transcription |
T11267 |
35736-35744 |
NN |
denotes |
Apterous |
T11268 |
35745-35748 |
CC |
denotes |
and |
T11269 |
35749-35756 |
NN |
denotes |
Squeeze |
T11270 |
35757-35758 |
-LRB- |
denotes |
[ |
T11271 |
35761-35763 |
CD |
denotes |
12 |
T11272 |
35758-35760 |
CD |
denotes |
11 |
T11273 |
35760-35761 |
, |
denotes |
, |
T11274 |
35763-35764 |
-RRB- |
denotes |
] |
T11275 |
35764-35765 |
. |
denotes |
. |
T11276 |
35765-35952 |
sentence |
denotes |
Thus, both in Drosophila and vertebrates, target-derived factors appear to act permissively to induce the expression of transcriptional programs involved in terminal neuronal maturation. |
T11277 |
35766-35770 |
RB |
denotes |
Thus |
T11278 |
35831-35837 |
VBP |
denotes |
appear |
T11279 |
35770-35772 |
, |
denotes |
, |
T11280 |
35772-35776 |
CC |
denotes |
both |
T11281 |
35777-35779 |
IN |
denotes |
in |
T11282 |
35780-35790 |
NNP |
denotes |
Drosophila |
T11283 |
35791-35794 |
CC |
denotes |
and |
T11284 |
35795-35806 |
NNS |
denotes |
vertebrates |
T11285 |
35806-35808 |
, |
denotes |
, |
T11286 |
35808-35814 |
NN |
denotes |
target |
T11287 |
35815-35822 |
VBN |
denotes |
derived |
T11288 |
35814-35815 |
HYPH |
denotes |
- |
T11289 |
35823-35830 |
NNS |
denotes |
factors |
T11290 |
35838-35840 |
TO |
denotes |
to |
T11291 |
35841-35844 |
VB |
denotes |
act |
T11292 |
35845-35857 |
RB |
denotes |
permissively |
T11293 |
35858-35860 |
TO |
denotes |
to |
T11294 |
35861-35867 |
VB |
denotes |
induce |
T11295 |
35868-35871 |
DT |
denotes |
the |
T11296 |
35872-35882 |
NN |
denotes |
expression |
T11297 |
35883-35885 |
IN |
denotes |
of |
T11298 |
35886-35901 |
JJ |
denotes |
transcriptional |
T11299 |
35902-35910 |
NNS |
denotes |
programs |
T11300 |
35911-35919 |
VBN |
denotes |
involved |
T11301 |
35920-35922 |
IN |
denotes |
in |
T11302 |
35923-35931 |
JJ |
denotes |
terminal |
T11303 |
35941-35951 |
NN |
denotes |
maturation |
T11304 |
35932-35940 |
JJ |
denotes |
neuronal |
T11305 |
35951-35952 |
. |
denotes |
. |
T11306 |
35952-36130 |
sentence |
denotes |
Our findings are compatible with a model in which DRG neurons acquire their mature fate by sequential and temporally controlled addition of lineage-specific features (Figure 9). |
T11307 |
35953-35956 |
PRP$ |
denotes |
Our |
T11308 |
35957-35965 |
NNS |
denotes |
findings |
T11309 |
35966-35969 |
VBP |
denotes |
are |
T11310 |
35970-35980 |
JJ |
denotes |
compatible |
T11311 |
35981-35985 |
IN |
denotes |
with |
T11312 |
35986-35987 |
DT |
denotes |
a |
T11313 |
35988-35993 |
NN |
denotes |
model |
T11314 |
35994-35996 |
IN |
denotes |
in |
T11315 |
36015-36022 |
VBP |
denotes |
acquire |
T11316 |
35997-36002 |
WDT |
denotes |
which |
T11317 |
36003-36006 |
NN |
denotes |
DRG |
T11318 |
36007-36014 |
NNS |
denotes |
neurons |
T11319 |
36023-36028 |
PRP$ |
denotes |
their |
T11320 |
36036-36040 |
NN |
denotes |
fate |
T11321 |
36029-36035 |
JJ |
denotes |
mature |
T11322 |
36041-36043 |
IN |
denotes |
by |
T11323 |
36044-36054 |
JJ |
denotes |
sequential |
T11324 |
36081-36089 |
NN |
denotes |
addition |
T11325 |
36055-36058 |
CC |
denotes |
and |
T11326 |
36059-36069 |
RB |
denotes |
temporally |
T11327 |
36070-36080 |
VBN |
denotes |
controlled |
T11328 |
36090-36092 |
IN |
denotes |
of |
T11329 |
36093-36100 |
NN |
denotes |
lineage |
T11330 |
36101-36109 |
JJ |
denotes |
specific |
T11331 |
36100-36101 |
HYPH |
denotes |
- |
T11332 |
36110-36118 |
NNS |
denotes |
features |
T11333 |
36119-36120 |
-LRB- |
denotes |
( |
T11334 |
36120-36126 |
NN |
denotes |
Figure |
T11335 |
36127-36128 |
CD |
denotes |
9 |
T11336 |
36128-36129 |
-RRB- |
denotes |
) |
T11337 |
36129-36130 |
. |
denotes |
. |
T11338 |
36130-36306 |
sentence |
denotes |
Target-derived factors act on predetermined neuronal lineages to switch their developmental programs to become compatible with processes such as target invasion and branching. |
T11339 |
36131-36137 |
NN |
denotes |
Target |
T11340 |
36138-36145 |
VBN |
denotes |
derived |
T11341 |
36137-36138 |
HYPH |
denotes |
- |
T11342 |
36146-36153 |
NNS |
denotes |
factors |
T11343 |
36154-36157 |
VBP |
denotes |
act |
T11344 |
36158-36160 |
IN |
denotes |
on |
T11345 |
36161-36174 |
VBN |
denotes |
predetermined |
T11346 |
36184-36192 |
NNS |
denotes |
lineages |
T11347 |
36175-36183 |
JJ |
denotes |
neuronal |
T11348 |
36193-36195 |
TO |
denotes |
to |
T11349 |
36196-36202 |
VB |
denotes |
switch |
T11350 |
36203-36208 |
PRP$ |
denotes |
their |
T11351 |
36223-36231 |
NNS |
denotes |
programs |
T11352 |
36209-36222 |
JJ |
denotes |
developmental |
T11353 |
36232-36234 |
TO |
denotes |
to |
T11354 |
36235-36241 |
VB |
denotes |
become |
T11355 |
36242-36252 |
JJ |
denotes |
compatible |
T11356 |
36253-36257 |
IN |
denotes |
with |
T11357 |
36258-36267 |
NNS |
denotes |
processes |
T11358 |
36268-36272 |
JJ |
denotes |
such |
T11359 |
36273-36275 |
IN |
denotes |
as |
T11360 |
36276-36282 |
NN |
denotes |
target |
T11361 |
36283-36291 |
NN |
denotes |
invasion |
T11362 |
36292-36295 |
CC |
denotes |
and |
T11363 |
36296-36305 |
NN |
denotes |
branching |
T11364 |
36305-36306 |
. |
denotes |
. |
T11365 |
36306-36512 |
sentence |
denotes |
Such a transition state in the acquisition of a defined neuronal fate would be accompanied by the induction of appropriate transcriptional programs through the expression of specific transcription factors. |
T11366 |
36307-36311 |
PDT |
denotes |
Such |
T11367 |
36325-36330 |
NN |
denotes |
state |
T11368 |
36312-36313 |
DT |
denotes |
a |
T11369 |
36314-36324 |
NN |
denotes |
transition |
T11370 |
36386-36397 |
VBN |
denotes |
accompanied |
T11371 |
36331-36333 |
IN |
denotes |
in |
T11372 |
36334-36337 |
DT |
denotes |
the |
T11373 |
36338-36349 |
NN |
denotes |
acquisition |
T11374 |
36350-36352 |
IN |
denotes |
of |
T11375 |
36353-36354 |
DT |
denotes |
a |
T11376 |
36372-36376 |
NN |
denotes |
fate |
T11377 |
36355-36362 |
VBN |
denotes |
defined |
T11378 |
36363-36371 |
JJ |
denotes |
neuronal |
T11379 |
36377-36382 |
MD |
denotes |
would |
T11380 |
36383-36385 |
VB |
denotes |
be |
T11381 |
36398-36400 |
IN |
denotes |
by |
T11382 |
36401-36404 |
DT |
denotes |
the |
T11383 |
36405-36414 |
NN |
denotes |
induction |
T11384 |
36415-36417 |
IN |
denotes |
of |
T11385 |
36418-36429 |
JJ |
denotes |
appropriate |
T11386 |
36446-36454 |
NNS |
denotes |
programs |
T11387 |
36430-36445 |
JJ |
denotes |
transcriptional |
T11388 |
36455-36462 |
IN |
denotes |
through |
T11389 |
36463-36466 |
DT |
denotes |
the |
T11390 |
36467-36477 |
NN |
denotes |
expression |
T11391 |
36478-36480 |
IN |
denotes |
of |
T11392 |
36481-36489 |
JJ |
denotes |
specific |
T11393 |
36504-36511 |
NNS |
denotes |
factors |
T11394 |
36490-36503 |
NN |
denotes |
transcription |
T11395 |
36511-36512 |
. |
denotes |
. |
T11396 |
36512-36916 |
sentence |
denotes |
Mechanisms such as chromosomal remodeling that restrict or expand access to certain target genes [34] or activation by cofactors responsible for changing the action of particular transcription factors [35] could represent possible mechanisms by which the downstream transcriptional profile of a transcription factor could be temporally shifted towards the selection and control of distinct target genes. |
T11397 |
36513-36523 |
NNS |
denotes |
Mechanisms |
T11398 |
36725-36734 |
VB |
denotes |
represent |
T11399 |
36524-36528 |
JJ |
denotes |
such |
T11400 |
36529-36531 |
IN |
denotes |
as |
T11401 |
36532-36543 |
JJ |
denotes |
chromosomal |
T11402 |
36544-36554 |
NN |
denotes |
remodeling |
T11403 |
36555-36559 |
WDT |
denotes |
that |
T11404 |
36560-36568 |
VB |
denotes |
restrict |
T11405 |
36569-36571 |
CC |
denotes |
or |
T11406 |
36572-36578 |
VB |
denotes |
expand |
T11407 |
36579-36585 |
NN |
denotes |
access |
T11408 |
36586-36588 |
IN |
denotes |
to |
T11409 |
36589-36596 |
JJ |
denotes |
certain |
T11410 |
36604-36609 |
NNS |
denotes |
genes |
T11411 |
36597-36603 |
NN |
denotes |
target |
T11412 |
36610-36611 |
-LRB- |
denotes |
[ |
T11413 |
36611-36613 |
CD |
denotes |
34 |
T11414 |
36613-36614 |
-RRB- |
denotes |
] |
T11415 |
36615-36617 |
CC |
denotes |
or |
T11416 |
36618-36628 |
NN |
denotes |
activation |
T11417 |
36629-36631 |
IN |
denotes |
by |
T11418 |
36632-36641 |
NNS |
denotes |
cofactors |
T11419 |
36642-36653 |
JJ |
denotes |
responsible |
T11420 |
36654-36657 |
IN |
denotes |
for |
T11421 |
36658-36666 |
VBG |
denotes |
changing |
T11422 |
36667-36670 |
DT |
denotes |
the |
T11423 |
36671-36677 |
NN |
denotes |
action |
T11424 |
36678-36680 |
IN |
denotes |
of |
T11425 |
36681-36691 |
JJ |
denotes |
particular |
T11426 |
36706-36713 |
NNS |
denotes |
factors |
T11427 |
36692-36705 |
NN |
denotes |
transcription |
T11428 |
36714-36715 |
-LRB- |
denotes |
[ |
T11429 |
36715-36717 |
CD |
denotes |
35 |
T11430 |
36717-36718 |
-RRB- |
denotes |
] |
T11431 |
36719-36724 |
MD |
denotes |
could |
T11432 |
36735-36743 |
JJ |
denotes |
possible |
T11433 |
36744-36754 |
NNS |
denotes |
mechanisms |
T11434 |
36755-36757 |
IN |
denotes |
by |
T11435 |
36849-36856 |
VBN |
denotes |
shifted |
T11436 |
36758-36763 |
WDT |
denotes |
which |
T11437 |
36764-36767 |
DT |
denotes |
the |
T11438 |
36795-36802 |
NN |
denotes |
profile |
T11439 |
36768-36778 |
JJ |
denotes |
downstream |
T11440 |
36779-36794 |
JJ |
denotes |
transcriptional |
T11441 |
36803-36805 |
IN |
denotes |
of |
T11442 |
36806-36807 |
DT |
denotes |
a |
T11443 |
36822-36828 |
NN |
denotes |
factor |
T11444 |
36808-36821 |
NN |
denotes |
transcription |
T11445 |
36829-36834 |
MD |
denotes |
could |
T11446 |
36835-36837 |
VB |
denotes |
be |
T11447 |
36838-36848 |
RB |
denotes |
temporally |
T11448 |
36857-36864 |
IN |
denotes |
towards |
T11449 |
36865-36868 |
DT |
denotes |
the |
T11450 |
36869-36878 |
NN |
denotes |
selection |
T11451 |
36879-36882 |
CC |
denotes |
and |
T11452 |
36883-36890 |
NN |
denotes |
control |
T11453 |
36891-36893 |
IN |
denotes |
of |
T11454 |
36894-36902 |
JJ |
denotes |
distinct |
T11455 |
36910-36915 |
NNS |
denotes |
genes |
T11456 |
36903-36909 |
NN |
denotes |
target |
T11457 |
36915-36916 |
. |
denotes |
. |
T11458 |
36916-37100 |
sentence |
denotes |
Interestingly, several ETS transcription factors are activated through release of autoinhibition via interaction with cofactors and/or via post-translational modifications [35,36,37]. |
T11459 |
36917-36930 |
RB |
denotes |
Interestingly |
T11460 |
36970-36979 |
VBN |
denotes |
activated |
T11461 |
36930-36932 |
, |
denotes |
, |
T11462 |
36932-36939 |
JJ |
denotes |
several |
T11463 |
36958-36965 |
NNS |
denotes |
factors |
T11464 |
36940-36943 |
NN |
denotes |
ETS |
T11465 |
36944-36957 |
NN |
denotes |
transcription |
T11466 |
36966-36969 |
VBP |
denotes |
are |
T11467 |
36980-36987 |
IN |
denotes |
through |
T11468 |
36988-36995 |
NN |
denotes |
release |
T11469 |
36996-36998 |
IN |
denotes |
of |
T11470 |
36999-37013 |
NN |
denotes |
autoinhibition |
T11471 |
37014-37017 |
IN |
denotes |
via |
T11472 |
37018-37029 |
NN |
denotes |
interaction |
T11473 |
37030-37034 |
IN |
denotes |
with |
T11474 |
37035-37044 |
NNS |
denotes |
cofactors |
T11475 |
37045-37048 |
CC |
denotes |
and |
T11476 |
37048-37049 |
HYPH |
denotes |
/ |
T11477 |
37049-37051 |
CC |
denotes |
or |
T11478 |
37052-37055 |
IN |
denotes |
via |
T11479 |
37056-37074 |
JJ |
denotes |
post-translational |
T11480 |
37075-37088 |
NNS |
denotes |
modifications |
T11481 |
37089-37090 |
-LRB- |
denotes |
[ |
T11482 |
37096-37098 |
CD |
denotes |
37 |
T11483 |
37090-37092 |
CD |
denotes |
35 |
T11484 |
37092-37093 |
, |
denotes |
, |
T11485 |
37093-37095 |
CD |
denotes |
36 |
T11486 |
37095-37096 |
, |
denotes |
, |
T11487 |
37098-37099 |
-RRB- |
denotes |
] |
T11488 |
37099-37100 |
. |
denotes |
. |
T11489 |
37100-37345 |
sentence |
denotes |
The fusion of EWS with Pea3 could circumvent a need for activation through specific cofactors while still maintaining ETS site dependence, thus rendering EWS-Pea3 less sensitive to the cellular context than endogenous ETS transcription factors. |
T11490 |
37101-37104 |
DT |
denotes |
The |
T11491 |
37105-37111 |
NN |
denotes |
fusion |
T11492 |
37135-37145 |
VB |
denotes |
circumvent |
T11493 |
37112-37114 |
IN |
denotes |
of |
T11494 |
37115-37118 |
NN |
denotes |
EWS |
T11495 |
37119-37123 |
IN |
denotes |
with |
T11496 |
37124-37128 |
NN |
denotes |
Pea3 |
T11497 |
37129-37134 |
MD |
denotes |
could |
T11498 |
37146-37147 |
DT |
denotes |
a |
T11499 |
37148-37152 |
NN |
denotes |
need |
T11500 |
37153-37156 |
IN |
denotes |
for |
T11501 |
37157-37167 |
NN |
denotes |
activation |
T11502 |
37168-37175 |
IN |
denotes |
through |
T11503 |
37176-37184 |
JJ |
denotes |
specific |
T11504 |
37185-37194 |
NNS |
denotes |
cofactors |
T11505 |
37195-37200 |
IN |
denotes |
while |
T11506 |
37207-37218 |
VBG |
denotes |
maintaining |
T11507 |
37201-37206 |
RB |
denotes |
still |
T11508 |
37219-37222 |
NN |
denotes |
ETS |
T11509 |
37228-37238 |
NN |
denotes |
dependence |
T11510 |
37223-37227 |
NN |
denotes |
site |
T11511 |
37238-37240 |
, |
denotes |
, |
T11512 |
37240-37244 |
RB |
denotes |
thus |
T11513 |
37245-37254 |
VBG |
denotes |
rendering |
T11514 |
37255-37258 |
NN |
denotes |
EWS |
T11515 |
37259-37263 |
NN |
denotes |
Pea3 |
T11516 |
37258-37259 |
HYPH |
denotes |
- |
T11517 |
37264-37268 |
RBR |
denotes |
less |
T11518 |
37269-37278 |
JJ |
denotes |
sensitive |
T11519 |
37279-37281 |
IN |
denotes |
to |
T11520 |
37282-37285 |
DT |
denotes |
the |
T11521 |
37295-37302 |
NN |
denotes |
context |
T11522 |
37286-37294 |
JJ |
denotes |
cellular |
T11523 |
37303-37307 |
IN |
denotes |
than |
T11524 |
37308-37318 |
JJ |
denotes |
endogenous |
T11525 |
37337-37344 |
NNS |
denotes |
factors |
T11526 |
37319-37322 |
NN |
denotes |
ETS |
T11527 |
37323-37336 |
NN |
denotes |
transcription |
T11528 |
37344-37345 |
. |
denotes |
. |
T11529 |
37345-37535 |
sentence |
denotes |
Using this fusion protein, our experiments demonstrate a profound change in the action of ETS signaling at the level of transcriptional regulation within post-mitotic DRG neurons over time. |
T11530 |
37346-37351 |
VBG |
denotes |
Using |
T11531 |
37389-37400 |
VBP |
denotes |
demonstrate |
T11532 |
37352-37356 |
DT |
denotes |
this |
T11533 |
37364-37371 |
NN |
denotes |
protein |
T11534 |
37357-37363 |
NN |
denotes |
fusion |
T11535 |
37371-37373 |
, |
denotes |
, |
T11536 |
37373-37376 |
PRP$ |
denotes |
our |
T11537 |
37377-37388 |
NNS |
denotes |
experiments |
T11538 |
37401-37402 |
DT |
denotes |
a |
T11539 |
37412-37418 |
NN |
denotes |
change |
T11540 |
37403-37411 |
JJ |
denotes |
profound |
T11541 |
37419-37421 |
IN |
denotes |
in |
T11542 |
37422-37425 |
DT |
denotes |
the |
T11543 |
37426-37432 |
NN |
denotes |
action |
T11544 |
37433-37435 |
IN |
denotes |
of |
T11545 |
37436-37439 |
NN |
denotes |
ETS |
T11546 |
37440-37449 |
NN |
denotes |
signaling |
T11547 |
37450-37452 |
IN |
denotes |
at |
T11548 |
37453-37456 |
DT |
denotes |
the |
T11549 |
37457-37462 |
NN |
denotes |
level |
T11550 |
37463-37465 |
IN |
denotes |
of |
T11551 |
37466-37481 |
JJ |
denotes |
transcriptional |
T11552 |
37482-37492 |
NN |
denotes |
regulation |
T11553 |
37493-37499 |
IN |
denotes |
within |
T11554 |
37500-37512 |
JJ |
denotes |
post-mitotic |
T11555 |
37517-37524 |
NNS |
denotes |
neurons |
T11556 |
37513-37516 |
NN |
denotes |
DRG |
T11557 |
37525-37529 |
IN |
denotes |
over |
T11558 |
37530-37534 |
NN |
denotes |
time |
T11559 |
37534-37535 |
. |
denotes |
. |
T11560 |
37535-37744 |
sentence |
denotes |
Moreover, the observed transcriptional shift in ETS signaling is paired with the onset of appropriate regulation of neuronal subtype specification and establishment of axonal projections into the target area. |
T11561 |
37536-37544 |
RB |
denotes |
Moreover |
T11562 |
37601-37607 |
VBN |
denotes |
paired |
T11563 |
37544-37546 |
, |
denotes |
, |
T11564 |
37546-37549 |
DT |
denotes |
the |
T11565 |
37575-37580 |
NN |
denotes |
shift |
T11566 |
37550-37558 |
VBN |
denotes |
observed |
T11567 |
37559-37574 |
JJ |
denotes |
transcriptional |
T11568 |
37581-37583 |
IN |
denotes |
in |
T11569 |
37584-37587 |
NN |
denotes |
ETS |
T11570 |
37588-37597 |
NN |
denotes |
signaling |
T11571 |
37598-37600 |
VBZ |
denotes |
is |
T11572 |
37608-37612 |
IN |
denotes |
with |
T11573 |
37613-37616 |
DT |
denotes |
the |
T11574 |
37617-37622 |
NN |
denotes |
onset |
T11575 |
37623-37625 |
IN |
denotes |
of |
T11576 |
37626-37637 |
JJ |
denotes |
appropriate |
T11577 |
37638-37648 |
NN |
denotes |
regulation |
T11578 |
37649-37651 |
IN |
denotes |
of |
T11579 |
37652-37660 |
JJ |
denotes |
neuronal |
T11580 |
37669-37682 |
NN |
denotes |
specification |
T11581 |
37661-37668 |
NN |
denotes |
subtype |
T11582 |
37683-37686 |
CC |
denotes |
and |
T11583 |
37687-37700 |
NN |
denotes |
establishment |
T11584 |
37701-37703 |
IN |
denotes |
of |
T11585 |
37704-37710 |
JJ |
denotes |
axonal |
T11586 |
37711-37722 |
NNS |
denotes |
projections |
T11587 |
37723-37727 |
IN |
denotes |
into |
T11588 |
37728-37731 |
DT |
denotes |
the |
T11589 |
37739-37743 |
NN |
denotes |
area |
T11590 |
37732-37738 |
NN |
denotes |
target |
T11591 |
37743-37744 |
. |
denotes |
. |
T11592 |
37744-38055 |
sentence |
denotes |
Recent experiments addressing the temporal constraints of transcription factor action in proliferating neural progenitor cells adds to the idea that defined temporal windows, during which transcription factors act to control distinct downstream target genes, are of key importance to neuronal fate acquisition. |
T11593 |
37745-37751 |
JJ |
denotes |
Recent |
T11594 |
37752-37763 |
NNS |
denotes |
experiments |
T11595 |
37872-37876 |
VBZ |
denotes |
adds |
T11596 |
37764-37774 |
VBG |
denotes |
addressing |
T11597 |
37775-37778 |
DT |
denotes |
the |
T11598 |
37788-37799 |
NNS |
denotes |
constraints |
T11599 |
37779-37787 |
JJ |
denotes |
temporal |
T11600 |
37800-37802 |
IN |
denotes |
of |
T11601 |
37803-37816 |
NN |
denotes |
transcription |
T11602 |
37817-37823 |
NN |
denotes |
factor |
T11603 |
37824-37830 |
NN |
denotes |
action |
T11604 |
37831-37833 |
IN |
denotes |
in |
T11605 |
37834-37847 |
VBG |
denotes |
proliferating |
T11606 |
37866-37871 |
NNS |
denotes |
cells |
T11607 |
37848-37854 |
JJ |
denotes |
neural |
T11608 |
37855-37865 |
NN |
denotes |
progenitor |
T11609 |
37877-37879 |
IN |
denotes |
to |
T11610 |
37880-37883 |
DT |
denotes |
the |
T11611 |
37884-37888 |
NN |
denotes |
idea |
T11612 |
37889-37893 |
IN |
denotes |
that |
T11613 |
38004-38007 |
VBP |
denotes |
are |
T11614 |
37894-37901 |
VBN |
denotes |
defined |
T11615 |
37911-37918 |
NNS |
denotes |
windows |
T11616 |
37902-37910 |
JJ |
denotes |
temporal |
T11617 |
37918-37920 |
, |
denotes |
, |
T11618 |
37920-37926 |
IN |
denotes |
during |
T11619 |
37955-37958 |
VBP |
denotes |
act |
T11620 |
37927-37932 |
WDT |
denotes |
which |
T11621 |
37933-37946 |
NN |
denotes |
transcription |
T11622 |
37947-37954 |
NNS |
denotes |
factors |
T11623 |
37959-37961 |
TO |
denotes |
to |
T11624 |
37962-37969 |
VB |
denotes |
control |
T11625 |
37970-37978 |
JJ |
denotes |
distinct |
T11626 |
37997-38002 |
NNS |
denotes |
genes |
T11627 |
37979-37989 |
JJ |
denotes |
downstream |
T11628 |
37990-37996 |
NN |
denotes |
target |
T11629 |
38002-38004 |
, |
denotes |
, |
T11630 |
38008-38010 |
IN |
denotes |
of |
T11631 |
38011-38014 |
JJ |
denotes |
key |
T11632 |
38015-38025 |
NN |
denotes |
importance |
T11633 |
38026-38028 |
IN |
denotes |
to |
T11634 |
38029-38037 |
JJ |
denotes |
neuronal |
T11635 |
38043-38054 |
NN |
denotes |
acquisition |
T11636 |
38038-38042 |
NN |
denotes |
fate |
T11637 |
38054-38055 |
. |
denotes |
. |
T11638 |
38055-38206 |
sentence |
denotes |
During Drosophila neuroblast generation, the transcription factor Hunchback controls specification and differentiation of early-born neuroblasts [38]. |
T11639 |
38056-38062 |
IN |
denotes |
During |
T11640 |
38132-38140 |
VBZ |
denotes |
controls |
T11641 |
38063-38073 |
NNP |
denotes |
Drosophila |
T11642 |
38085-38095 |
NN |
denotes |
generation |
T11643 |
38074-38084 |
NN |
denotes |
neuroblast |
T11644 |
38095-38097 |
, |
denotes |
, |
T11645 |
38097-38100 |
DT |
denotes |
the |
T11646 |
38122-38131 |
NN |
denotes |
Hunchback |
T11647 |
38101-38114 |
NN |
denotes |
transcription |
T11648 |
38115-38121 |
NN |
denotes |
factor |
T11649 |
38141-38154 |
NN |
denotes |
specification |
T11650 |
38155-38158 |
CC |
denotes |
and |
T11651 |
38159-38174 |
NN |
denotes |
differentiation |
T11652 |
38175-38177 |
IN |
denotes |
of |
T11653 |
38178-38183 |
RB |
denotes |
early |
T11654 |
38184-38188 |
VBN |
denotes |
born |
T11655 |
38183-38184 |
HYPH |
denotes |
- |
T11656 |
38189-38200 |
NNS |
denotes |
neuroblasts |
T11657 |
38201-38202 |
-LRB- |
denotes |
[ |
T11658 |
38202-38204 |
CD |
denotes |
38 |
T11659 |
38204-38205 |
-RRB- |
denotes |
] |
T11660 |
38205-38206 |
. |
denotes |
. |
T11661 |
38206-38351 |
sentence |
denotes |
Over time, however, neuroblasts progressively lose their competence to generate cells of an early fate in response to Hunchback expression [39]. |
T11662 |
38207-38211 |
IN |
denotes |
Over |
T11663 |
38253-38257 |
VBP |
denotes |
lose |
T11664 |
38212-38216 |
NN |
denotes |
time |
T11665 |
38216-38218 |
, |
denotes |
, |
T11666 |
38218-38225 |
RB |
denotes |
however |
T11667 |
38225-38227 |
, |
denotes |
, |
T11668 |
38227-38238 |
NNS |
denotes |
neuroblasts |
T11669 |
38239-38252 |
RB |
denotes |
progressively |
T11670 |
38258-38263 |
PRP$ |
denotes |
their |
T11671 |
38264-38274 |
NN |
denotes |
competence |
T11672 |
38275-38277 |
TO |
denotes |
to |
T11673 |
38278-38286 |
VB |
denotes |
generate |
T11674 |
38287-38292 |
NNS |
denotes |
cells |
T11675 |
38293-38295 |
IN |
denotes |
of |
T11676 |
38296-38298 |
DT |
denotes |
an |
T11677 |
38305-38309 |
NN |
denotes |
fate |
T11678 |
38299-38304 |
JJ |
denotes |
early |
T11679 |
38310-38312 |
IN |
denotes |
in |
T11680 |
38313-38321 |
NN |
denotes |
response |
T11681 |
38322-38324 |
IN |
denotes |
to |
T11682 |
38325-38334 |
NN |
denotes |
Hunchback |
T11683 |
38335-38345 |
NN |
denotes |
expression |
T11684 |
38346-38347 |
-LRB- |
denotes |
[ |
T11685 |
38347-38349 |
CD |
denotes |
39 |
T11686 |
38349-38350 |
-RRB- |
denotes |
] |
T11687 |
38350-38351 |
. |
denotes |
. |
T11688 |
38351-38512 |
sentence |
denotes |
These findings thus also argue for a change in cellular competence to respond to a specific transcription factor over time albeit in an early precursor context. |
T11689 |
38352-38357 |
DT |
denotes |
These |
T11690 |
38358-38366 |
NNS |
denotes |
findings |
T11691 |
38377-38382 |
VBP |
denotes |
argue |
T11692 |
38367-38371 |
RB |
denotes |
thus |
T11693 |
38372-38376 |
RB |
denotes |
also |
T11694 |
38383-38386 |
IN |
denotes |
for |
T11695 |
38387-38388 |
DT |
denotes |
a |
T11696 |
38389-38395 |
NN |
denotes |
change |
T11697 |
38396-38398 |
IN |
denotes |
in |
T11698 |
38399-38407 |
JJ |
denotes |
cellular |
T11699 |
38408-38418 |
NN |
denotes |
competence |
T11700 |
38419-38421 |
TO |
denotes |
to |
T11701 |
38422-38429 |
VB |
denotes |
respond |
T11702 |
38430-38432 |
IN |
denotes |
to |
T11703 |
38433-38434 |
DT |
denotes |
a |
T11704 |
38458-38464 |
NN |
denotes |
factor |
T11705 |
38435-38443 |
JJ |
denotes |
specific |
T11706 |
38444-38457 |
NN |
denotes |
transcription |
T11707 |
38465-38469 |
IN |
denotes |
over |
T11708 |
38470-38474 |
NN |
denotes |
time |
T11709 |
38475-38481 |
IN |
denotes |
albeit |
T11710 |
38482-38484 |
IN |
denotes |
in |
T11711 |
38485-38487 |
DT |
denotes |
an |
T11712 |
38504-38511 |
NN |
denotes |
context |
T11713 |
38488-38493 |
JJ |
denotes |
early |
T11714 |
38494-38503 |
NN |
denotes |
precursor |
T11715 |
38511-38512 |
. |
denotes |
. |
T11716 |
38512-38715 |
sentence |
denotes |
More generally, during the differentiation of hematopoietic lineages, several transcription factors have also been shown to exhibit distinct functions at progressive steps of lineage specification [40]. |
T11717 |
38513-38517 |
RBR |
denotes |
More |
T11718 |
38518-38527 |
RB |
denotes |
generally |
T11719 |
38628-38633 |
VBN |
denotes |
shown |
T11720 |
38527-38529 |
, |
denotes |
, |
T11721 |
38529-38535 |
IN |
denotes |
during |
T11722 |
38536-38539 |
DT |
denotes |
the |
T11723 |
38540-38555 |
NN |
denotes |
differentiation |
T11724 |
38556-38558 |
IN |
denotes |
of |
T11725 |
38559-38572 |
JJ |
denotes |
hematopoietic |
T11726 |
38573-38581 |
NNS |
denotes |
lineages |
T11727 |
38581-38583 |
, |
denotes |
, |
T11728 |
38583-38590 |
JJ |
denotes |
several |
T11729 |
38605-38612 |
NNS |
denotes |
factors |
T11730 |
38591-38604 |
NN |
denotes |
transcription |
T11731 |
38613-38617 |
VBP |
denotes |
have |
T11764 |
38807-38809 |
TO |
denotes |
to |
T11765 |
38810-38817 |
VB |
denotes |
control |
T11766 |
38818-38827 |
JJ |
denotes |
different |
T11767 |
38828-38839 |
NNS |
denotes |
complements |
T11768 |
38840-38842 |
IN |
denotes |
of |
T11769 |
38843-38853 |
JJ |
denotes |
downstream |
T11770 |
38854-38859 |
NNS |
denotes |
genes |
T11771 |
38860-38863 |
CC |
denotes |
and |
T11772 |
38864-38868 |
RB |
denotes |
thus |
T11773 |
38869-38878 |
JJ |
denotes |
different |
T11774 |
38879-38886 |
NNS |
denotes |
aspects |
T11775 |
38887-38889 |
IN |
denotes |
of |
T11776 |
38890-38898 |
JJ |
denotes |
neuronal |
T11777 |
38912-38917 |
NNS |
denotes |
fates |
T11778 |
38899-38902 |
CC |
denotes |
and |
T11779 |
38903-38911 |
JJ |
denotes |
cellular |
T11780 |
38918-38920 |
IN |
denotes |
in |
T11781 |
38921-38931 |
NN |
denotes |
progenitor |
T11782 |
38932-38937 |
NNS |
denotes |
cells |
T11783 |
38938-38940 |
CC |
denotes |
or |
T11784 |
38941-38953 |
JJ |
denotes |
post-mitotic |
T11785 |
38954-38961 |
NNS |
denotes |
neurons |
T11786 |
38962-38965 |
MD |
denotes |
may |
T11787 |
38974-38981 |
JJ |
denotes |
further |
T11788 |
38982-38989 |
NN |
denotes |
insight |
T11789 |
38990-38994 |
IN |
denotes |
into |
T11790 |
38995-38998 |
DT |
denotes |
the |
T11791 |
38999-39002 |
NN |
denotes |
way |
T11792 |
39003-39005 |
IN |
denotes |
in |
T11793 |
39034-39037 |
VBP |
denotes |
act |
T11794 |
39006-39011 |
WDT |
denotes |
which |
T11795 |
39012-39025 |
NN |
denotes |
transcription |
T11796 |
39026-39033 |
NNS |
denotes |
factors |
T11797 |
39038-39040 |
TO |
denotes |
to |
T11798 |
39041-39048 |
VB |
denotes |
control |
T11799 |
39049-39052 |
DT |
denotes |
the |
T11800 |
39053-39061 |
NN |
denotes |
assembly |
T11801 |
39062-39064 |
IN |
denotes |
of |
T11802 |
39065-39073 |
JJ |
denotes |
neuronal |
T11803 |
39074-39082 |
NNS |
denotes |
circuits |
T11804 |
39082-39083 |
. |
denotes |
. |
T12345 |
39108-39118 |
NN |
denotes |
Generation |
T12346 |
39119-39121 |
IN |
denotes |
of |
T12347 |
39122-39132 |
JJ |
denotes |
transgenic |
T12348 |
39133-39137 |
NNS |
denotes |
mice |
T12349 |
39138-39141 |
CC |
denotes |
and |
T12350 |
39142-39147 |
NN |
denotes |
mouse |
T12351 |
39148-39156 |
NN |
denotes |
genetics |
T12352 |
39156-39277 |
sentence |
denotes |
Er81EWS-Pea3 mice were generated following a strategy similar to that described for the generation of Er81NLZ mice [14]. |
T12353 |
39157-39164 |
NN |
denotes |
Er81EWS |
T12354 |
39165-39169 |
NN |
denotes |
Pea3 |
T12355 |
39164-39165 |
HYPH |
denotes |
- |
T12356 |
39170-39174 |
NNS |
denotes |
mice |
T12357 |
39180-39189 |
VBN |
denotes |
generated |
T12358 |
39175-39179 |
VBD |
denotes |
were |
T12359 |
39190-39199 |
VBG |
denotes |
following |
T12360 |
39200-39201 |
DT |
denotes |
a |
T12361 |
39202-39210 |
NN |
denotes |
strategy |
T12362 |
39211-39218 |
JJ |
denotes |
similar |
T12363 |
39219-39221 |
IN |
denotes |
to |
T12364 |
39222-39226 |
DT |
denotes |
that |
T12365 |
39227-39236 |
VBN |
denotes |
described |
T12366 |
39237-39240 |
IN |
denotes |
for |
T12367 |
39241-39244 |
DT |
denotes |
the |
T12368 |
39245-39255 |
NN |
denotes |
generation |
T12369 |
39256-39258 |
IN |
denotes |
of |
T12370 |
39259-39266 |
NN |
denotes |
Er81NLZ |
T12371 |
39267-39271 |
NNS |
denotes |
mice |
T12372 |
39272-39273 |
-LRB- |
denotes |
[ |
T12373 |
39273-39275 |
CD |
denotes |
14 |
T12374 |
39275-39276 |
-RRB- |
denotes |
] |
T12375 |
39276-39277 |
. |
denotes |
. |
T12376 |
39277-39483 |
sentence |
denotes |
In brief, a targeting vector with a cDNA coding for EWS-Pea3 was inserted in frame with the endogenous start ATG into exon 2 of the Er81 genomic locus and used for homologous recombination in W95 ES cells. |
T12377 |
39278-39280 |
IN |
denotes |
In |
T12378 |
39343-39351 |
VBN |
denotes |
inserted |
T12379 |
39281-39286 |
NN |
denotes |
brief |
T12380 |
39286-39288 |
, |
denotes |
, |
T12381 |
39288-39289 |
DT |
denotes |
a |
T12382 |
39300-39306 |
NN |
denotes |
vector |
T12383 |
39290-39299 |
NN |
denotes |
targeting |
T12384 |
39307-39311 |
IN |
denotes |
with |
T12385 |
39312-39313 |
DT |
denotes |
a |
T12386 |
39319-39325 |
NN |
denotes |
coding |
T12387 |
39314-39318 |
NN |
denotes |
cDNA |
T12388 |
39326-39329 |
IN |
denotes |
for |
T12389 |
39330-39333 |
NN |
denotes |
EWS |
T12390 |
39334-39338 |
NN |
denotes |
Pea3 |
T12391 |
39333-39334 |
HYPH |
denotes |
- |
T12392 |
39339-39342 |
VBD |
denotes |
was |
T12393 |
39352-39354 |
IN |
denotes |
in |
T12394 |
39355-39360 |
NN |
denotes |
frame |
T12395 |
39361-39365 |
IN |
denotes |
with |
T12396 |
39366-39369 |
DT |
denotes |
the |
T12397 |
39387-39390 |
NN |
denotes |
ATG |
T12398 |
39370-39380 |
JJ |
denotes |
endogenous |
T12399 |
39381-39386 |
NN |
denotes |
start |
T12400 |
39391-39395 |
IN |
denotes |
into |
T12401 |
39396-39400 |
NN |
denotes |
exon |
T12402 |
39401-39402 |
CD |
denotes |
2 |
T12403 |
39403-39405 |
IN |
denotes |
of |
T12404 |
39406-39409 |
DT |
denotes |
the |
T12405 |
39423-39428 |
NN |
denotes |
locus |
T12406 |
39410-39414 |
NN |
denotes |
Er81 |
T12407 |
39415-39422 |
JJ |
denotes |
genomic |
T12408 |
39429-39432 |
CC |
denotes |
and |
T12409 |
39433-39437 |
VBN |
denotes |
used |
T12410 |
39438-39441 |
IN |
denotes |
for |
T12411 |
39442-39452 |
JJ |
denotes |
homologous |
T12412 |
39453-39466 |
NN |
denotes |
recombination |
T12413 |
39467-39469 |
IN |
denotes |
in |
T12414 |
39470-39473 |
NN |
denotes |
W95 |
T12415 |
39477-39482 |
NNS |
denotes |
cells |
T12416 |
39474-39476 |
NN |
denotes |
ES |
T12417 |
39482-39483 |
. |
denotes |
. |
T12418 |
39483-39584 |
sentence |
denotes |
EWS-Pea3 represents a fusion gene between the amino terminal of EWS and the ETS domain of Pea3 [20]. |
T12419 |
39484-39487 |
NN |
denotes |
EWS |
T12420 |
39488-39492 |
NN |
denotes |
Pea3 |
T12421 |
39487-39488 |
HYPH |
denotes |
- |
T12422 |
39493-39503 |
VBZ |
denotes |
represents |
T12423 |
39504-39505 |
DT |
denotes |
a |
T12424 |
39513-39517 |
NN |
denotes |
gene |
T12425 |
39506-39512 |
NN |
denotes |
fusion |
T12426 |
39518-39525 |
IN |
denotes |
between |
T12427 |
39526-39529 |
DT |
denotes |
the |
T12428 |
39536-39544 |
NN |
denotes |
terminal |
T12429 |
39530-39535 |
NN |
denotes |
amino |
T12430 |
39545-39547 |
IN |
denotes |
of |
T12431 |
39548-39551 |
NN |
denotes |
EWS |
T12432 |
39552-39555 |
CC |
denotes |
and |
T12433 |
39556-39559 |
DT |
denotes |
the |
T12434 |
39564-39570 |
NN |
denotes |
domain |
T12435 |
39560-39563 |
NN |
denotes |
ETS |
T12436 |
39571-39573 |
IN |
denotes |
of |
T12437 |
39574-39578 |
NN |
denotes |
Pea3 |
T12438 |
39579-39580 |
-LRB- |
denotes |
[ |
T12439 |
39580-39582 |
CD |
denotes |
20 |
T12440 |
39582-39583 |
-RRB- |
denotes |
] |
T12441 |
39583-39584 |
. |
denotes |
. |
T12442 |
39584-39720 |
sentence |
denotes |
The primer pair used to specifically detect the Er81EWS-Pea3 allele was 5′- CAGCCACTGCACCTACAAGAC-3′ and 5′- CTTCCTGCTTGATGTCTCCTTC-3′. |
T12443 |
39585-39588 |
DT |
denotes |
The |
T12444 |
39596-39600 |
NN |
denotes |
pair |
T12445 |
39589-39595 |
NN |
denotes |
primer |
T12446 |
39653-39656 |
VBD |
denotes |
was |
T12447 |
39601-39605 |
VBN |
denotes |
used |
T12448 |
39606-39608 |
TO |
denotes |
to |
T12449 |
39622-39628 |
VB |
denotes |
detect |
T12450 |
39609-39621 |
RB |
denotes |
specifically |
T12451 |
39629-39632 |
DT |
denotes |
the |
T12452 |
39646-39652 |
NN |
denotes |
allele |
T12453 |
39633-39640 |
NN |
denotes |
Er81EWS |
T12454 |
39641-39645 |
NN |
denotes |
Pea3 |
T12455 |
39640-39641 |
HYPH |
denotes |
- |
T12456 |
39657-39658 |
CD |
denotes |
5 |
T12457 |
39661-39682 |
NN |
denotes |
CAGCCACTGCACCTACAAGAC |
T12458 |
39658-39659 |
SYM |
denotes |
′ |
T12459 |
39659-39660 |
HYPH |
denotes |
- |
T12460 |
39682-39683 |
HYPH |
denotes |
- |
T12461 |
39683-39684 |
CD |
denotes |
3 |
T12462 |
39684-39685 |
SYM |
denotes |
′ |
T12463 |
39686-39689 |
CC |
denotes |
and |
T12464 |
39690-39691 |
CD |
denotes |
5 |
T12465 |
39694-39716 |
NN |
denotes |
CTTCCTGCTTGATGTCTCCTTC |
T12466 |
39691-39692 |
SYM |
denotes |
′ |
T12467 |
39692-39693 |
HYPH |
denotes |
- |
T12468 |
39716-39717 |
HYPH |
denotes |
- |
T12469 |
39717-39718 |
CD |
denotes |
3 |
T12470 |
39718-39719 |
SYM |
denotes |
′ |
T12471 |
39719-39720 |
. |
denotes |
. |
T12472 |
39720-40020 |
sentence |
denotes |
For the generation of TaumGFP and TauEWS-Pea3 mice, lox-STOP-lox-mGFP-IRES-NLS-LacZ-pA and lox-STOP-lox-EWS-Pea3-IRES-NLS-LacZ-pA targeting cassettes were integrated into exon 2 of the Tau genomic locus (the endogenous start ATG was removed in the targeting vectors; details available upon request). |
T12473 |
39721-39724 |
IN |
denotes |
For |
T12474 |
39876-39886 |
VBN |
denotes |
integrated |
T12475 |
39725-39728 |
DT |
denotes |
the |
T12476 |
39729-39739 |
NN |
denotes |
generation |
T12477 |
39740-39742 |
IN |
denotes |
of |
T12478 |
39743-39750 |
NN |
denotes |
TaumGFP |
T12479 |
39767-39771 |
NNS |
denotes |
mice |
T12480 |
39751-39754 |
CC |
denotes |
and |
T12481 |
39755-39761 |
NN |
denotes |
TauEWS |
T12482 |
39762-39766 |
NN |
denotes |
Pea3 |
T12483 |
39761-39762 |
HYPH |
denotes |
- |
T12484 |
39771-39773 |
, |
denotes |
, |
T12485 |
39773-39776 |
NN |
denotes |
lox |
T12486 |
39805-39807 |
NN |
denotes |
pA |
T12487 |
39776-39777 |
HYPH |
denotes |
- |
T12488 |
39777-39781 |
NN |
denotes |
STOP |
T12489 |
39781-39782 |
HYPH |
denotes |
- |
T12490 |
39782-39785 |
NN |
denotes |
lox |
T12491 |
39785-39786 |
HYPH |
denotes |
- |
T12492 |
39786-39790 |
NN |
denotes |
mGFP |
T12493 |
39790-39791 |
HYPH |
denotes |
- |
T12494 |
39791-39795 |
NN |
denotes |
IRES |
T12495 |
39795-39796 |
HYPH |
denotes |
- |
T12496 |
39796-39799 |
NN |
denotes |
NLS |
T12497 |
39799-39800 |
HYPH |
denotes |
- |
T12498 |
39800-39804 |
NN |
denotes |
LacZ |
T12499 |
39804-39805 |
HYPH |
denotes |
- |
T12500 |
39861-39870 |
NNS |
denotes |
cassettes |
T12501 |
39808-39811 |
CC |
denotes |
and |
T12502 |
39812-39815 |
NN |
denotes |
lox |
T12503 |
39848-39850 |
NN |
denotes |
pA |
T12504 |
39815-39816 |
HYPH |
denotes |
- |
T12505 |
39816-39820 |
NN |
denotes |
STOP |
T12506 |
39820-39821 |
HYPH |
denotes |
- |
T12507 |
39821-39824 |
NN |
denotes |
lox |
T12508 |
39824-39825 |
HYPH |
denotes |
- |
T12509 |
39825-39828 |
NN |
denotes |
EWS |
T12510 |
39828-39829 |
HYPH |
denotes |
- |
T12511 |
39829-39833 |
NN |
denotes |
Pea3 |
T12512 |
39833-39834 |
HYPH |
denotes |
- |
T12513 |
39834-39838 |
NN |
denotes |
IRES |
T12514 |
39838-39839 |
HYPH |
denotes |
- |
T12515 |
39839-39842 |
NN |
denotes |
NLS |
T12516 |
39842-39843 |
HYPH |
denotes |
- |
T12517 |
39843-39847 |
NN |
denotes |
LacZ |
T12518 |
39847-39848 |
HYPH |
denotes |
- |
T12519 |
39851-39860 |
NN |
denotes |
targeting |
T12520 |
39871-39875 |
VBD |
denotes |
were |
T12521 |
39887-39891 |
IN |
denotes |
into |
T12522 |
39892-39896 |
NN |
denotes |
exon |
T12523 |
39897-39898 |
CD |
denotes |
2 |
T12524 |
39899-39901 |
IN |
denotes |
of |
T12525 |
39902-39905 |
DT |
denotes |
the |
T12526 |
39918-39923 |
NN |
denotes |
locus |
T12527 |
39906-39909 |
NN |
denotes |
Tau |
T12528 |
39910-39917 |
JJ |
denotes |
genomic |
T12529 |
39924-39925 |
-LRB- |
denotes |
( |
T12530 |
39954-39961 |
VBN |
denotes |
removed |
T12531 |
39925-39928 |
DT |
denotes |
the |
T12532 |
39946-39949 |
NN |
denotes |
ATG |
T12533 |
39929-39939 |
JJ |
denotes |
endogenous |
T12534 |
39940-39945 |
NN |
denotes |
start |
T12535 |
39950-39953 |
VBD |
denotes |
was |
T12536 |
39962-39964 |
IN |
denotes |
in |
T12537 |
39965-39968 |
DT |
denotes |
the |
T12538 |
39979-39986 |
NNS |
denotes |
vectors |
T12539 |
39969-39978 |
NN |
denotes |
targeting |
T12540 |
39986-39987 |
: |
denotes |
; |
T12541 |
39988-39995 |
NNS |
denotes |
details |
T12542 |
39996-40005 |
JJ |
denotes |
available |
T12543 |
40006-40010 |
IN |
denotes |
upon |
T12544 |
40011-40018 |
NN |
denotes |
request |
T12545 |
40018-40019 |
-RRB- |
denotes |
) |
T12546 |
40019-40020 |
. |
denotes |
. |
T12547 |
40020-40057 |
sentence |
denotes |
mGFP was provided by P. Caroni [25]. |
T12548 |
40021-40025 |
NN |
denotes |
mGFP |
T12549 |
40030-40038 |
VBN |
denotes |
provided |
T12550 |
40026-40029 |
VBD |
denotes |
was |
T12551 |
40039-40041 |
IN |
denotes |
by |
T12552 |
40042-40044 |
NNP |
denotes |
P. |
T12553 |
40045-40051 |
NNP |
denotes |
Caroni |
T12554 |
40052-40053 |
-LRB- |
denotes |
[ |
T12555 |
40053-40055 |
CD |
denotes |
25 |
T12556 |
40055-40056 |
-RRB- |
denotes |
] |
T12557 |
40056-40057 |
. |
denotes |
. |
T12558 |
40057-40181 |
sentence |
denotes |
ES cell recombinants were screened by Southern blot analysis using the probe in the 5′ region as described previously [41]. |
T12559 |
40058-40060 |
NN |
denotes |
ES |
T12560 |
40066-40078 |
NNS |
denotes |
recombinants |
T12561 |
40061-40065 |
NN |
denotes |
cell |
T12562 |
40084-40092 |
VBN |
denotes |
screened |
T12563 |
40079-40083 |
VBD |
denotes |
were |
T12564 |
40093-40095 |
IN |
denotes |
by |
T12565 |
40096-40104 |
NNP |
denotes |
Southern |
T12566 |
40105-40109 |
NN |
denotes |
blot |
T12567 |
40110-40118 |
NN |
denotes |
analysis |
T12568 |
40119-40124 |
VBG |
denotes |
using |
T12569 |
40125-40128 |
DT |
denotes |
the |
T12570 |
40129-40134 |
NN |
denotes |
probe |
T12571 |
40135-40137 |
IN |
denotes |
in |
T12572 |
40138-40141 |
DT |
denotes |
the |
T12573 |
40145-40151 |
NN |
denotes |
region |
T12574 |
40142-40143 |
CD |
denotes |
5 |
T12575 |
40143-40144 |
SYM |
denotes |
′ |
T12576 |
40152-40154 |
IN |
denotes |
as |
T12577 |
40155-40164 |
VBN |
denotes |
described |
T12578 |
40165-40175 |
RB |
denotes |
previously |
T12579 |
40176-40177 |
-LRB- |
denotes |
[ |
T12580 |
40177-40179 |
CD |
denotes |
41 |
T12581 |
40179-40180 |
-RRB- |
denotes |
] |
T12582 |
40180-40181 |
. |
denotes |
. |
T12583 |
40181-40275 |
sentence |
denotes |
Frequency of recombination in 129/Ola ES cells was approximately 1/3 for both Tau constructs. |
T12584 |
40182-40191 |
NN |
denotes |
Frequency |
T12585 |
40229-40232 |
VBD |
denotes |
was |
T12586 |
40192-40194 |
IN |
denotes |
of |
T12587 |
40195-40208 |
NN |
denotes |
recombination |
T12588 |
40209-40211 |
IN |
denotes |
in |
T12589 |
40212-40215 |
CD |
denotes |
129 |
T12590 |
40216-40219 |
NN |
denotes |
Ola |
T12591 |
40215-40216 |
HYPH |
denotes |
/ |
T12592 |
40223-40228 |
NNS |
denotes |
cells |
T12593 |
40220-40222 |
NN |
denotes |
ES |
T12594 |
40233-40246 |
RB |
denotes |
approximately |
T12595 |
40249-40250 |
CD |
denotes |
3 |
T12596 |
40247-40248 |
CD |
denotes |
1 |
T12597 |
40248-40249 |
SYM |
denotes |
/ |
T12598 |
40251-40254 |
IN |
denotes |
for |
T12599 |
40255-40259 |
DT |
denotes |
both |
T12600 |
40264-40274 |
NNS |
denotes |
constructs |
T12601 |
40260-40263 |
NN |
denotes |
Tau |
T12602 |
40274-40275 |
. |
denotes |
. |
T12603 |
40275-40430 |
sentence |
denotes |
For the generation of PVCre mice, mouse genomic clones were obtained by screening a 129SV/J genomic library (Incyte, Wilmington, Delaware, United States). |
T12604 |
40276-40279 |
IN |
denotes |
For |
T12605 |
40336-40344 |
VBN |
denotes |
obtained |
T12606 |
40280-40283 |
DT |
denotes |
the |
T12607 |
40284-40294 |
NN |
denotes |
generation |
T12608 |
40295-40297 |
IN |
denotes |
of |
T12609 |
40298-40303 |
NN |
denotes |
PVCre |
T12610 |
40304-40308 |
NNS |
denotes |
mice |
T12611 |
40308-40310 |
, |
denotes |
, |
T12612 |
40310-40315 |
NN |
denotes |
mouse |
T12613 |
40324-40330 |
NNS |
denotes |
clones |
T12614 |
40316-40323 |
JJ |
denotes |
genomic |
T12615 |
40331-40335 |
VBD |
denotes |
were |
T12616 |
40345-40347 |
IN |
denotes |
by |
T12617 |
40348-40357 |
VBG |
denotes |
screening |
T12618 |
40358-40359 |
DT |
denotes |
a |
T12619 |
40376-40383 |
NN |
denotes |
library |
T12620 |
40360-40365 |
NN |
denotes |
129SV |
T12621 |
40366-40367 |
NN |
denotes |
J |
T12622 |
40365-40366 |
HYPH |
denotes |
/ |
T12623 |
40368-40375 |
JJ |
denotes |
genomic |
T12624 |
40384-40385 |
-LRB- |
denotes |
( |
T12625 |
40385-40391 |
NNP |
denotes |
Incyte |
T12626 |
40391-40393 |
, |
denotes |
, |
T12627 |
40393-40403 |
NNP |
denotes |
Wilmington |
T12628 |
40403-40405 |
, |
denotes |
, |
T12629 |
40405-40413 |
NNP |
denotes |
Delaware |
T12630 |
40413-40415 |
, |
denotes |
, |
T12631 |
40415-40421 |
NNP |
denotes |
United |
T12632 |
40422-40428 |
NNP |
denotes |
States |
T12633 |
40428-40429 |
-RRB- |
denotes |
) |
T12634 |
40429-40430 |
. |
denotes |
. |
T12635 |
40430-40499 |
sentence |
denotes |
For details on the genomic structure of the mouse PV locus see [42]. |
T12636 |
40431-40434 |
IN |
denotes |
For |
T12637 |
40490-40493 |
VBP |
denotes |
see |
T12638 |
40435-40442 |
NNS |
denotes |
details |
T12639 |
40443-40445 |
IN |
denotes |
on |
T12640 |
40446-40449 |
DT |
denotes |
the |
T12641 |
40458-40467 |
NN |
denotes |
structure |
T12642 |
40450-40457 |
JJ |
denotes |
genomic |
T12643 |
40468-40470 |
IN |
denotes |
of |
T12644 |
40471-40474 |
DT |
denotes |
the |
T12645 |
40484-40489 |
NN |
denotes |
locus |
T12646 |
40475-40480 |
NN |
denotes |
mouse |
T12647 |
40481-40483 |
NN |
denotes |
PV |
T12648 |
40494-40495 |
-LRB- |
denotes |
[ |
T12649 |
40495-40497 |
CD |
denotes |
42 |
T12650 |
40497-40498 |
-RRB- |
denotes |
] |
T12651 |
40498-40499 |
. |
denotes |
. |
T12652 |
40499-40743 |
sentence |
denotes |
An IRES-Cre-pA targeting cassette [33] was integrated into the 3′ UTR of exon 5, and ES cell recombinants were screened with a 5′ probe (oligos, 5′- GAGATGACCCAGCCAGGATGCCTC-3′ and 5′- CTGACCACTCTCGCTCCGGTGTCC-3′; genomic DNA, HindIII digest). |
T12653 |
40500-40502 |
DT |
denotes |
An |
T12654 |
40525-40533 |
NN |
denotes |
cassette |
T12655 |
40503-40507 |
NN |
denotes |
IRES |
T12656 |
40512-40514 |
NN |
denotes |
pA |
T12657 |
40507-40508 |
HYPH |
denotes |
- |
T12658 |
40508-40511 |
NN |
denotes |
Cre |
T12659 |
40511-40512 |
HYPH |
denotes |
- |
T12660 |
40515-40524 |
NN |
denotes |
targeting |
T12661 |
40543-40553 |
VBN |
denotes |
integrated |
T12662 |
40534-40535 |
-LRB- |
denotes |
[ |
T12663 |
40535-40537 |
CD |
denotes |
33 |
T12664 |
40537-40538 |
-RRB- |
denotes |
] |
T12665 |
40539-40542 |
VBD |
denotes |
was |
T12666 |
40554-40558 |
IN |
denotes |
into |
T12667 |
40559-40562 |
DT |
denotes |
the |
T12668 |
40566-40569 |
NN |
denotes |
UTR |
T12669 |
40563-40564 |
CD |
denotes |
3 |
T12670 |
40564-40565 |
SYM |
denotes |
′ |
T12671 |
40570-40572 |
IN |
denotes |
of |
T12672 |
40573-40577 |
NN |
denotes |
exon |
T12673 |
40578-40579 |
CD |
denotes |
5 |
T12674 |
40579-40581 |
, |
denotes |
, |
T12675 |
40581-40584 |
CC |
denotes |
and |
T12676 |
40585-40587 |
NN |
denotes |
ES |
T12677 |
40593-40605 |
NNS |
denotes |
recombinants |
T12678 |
40588-40592 |
NN |
denotes |
cell |
T12679 |
40611-40619 |
VBN |
denotes |
screened |
T12680 |
40606-40610 |
VBD |
denotes |
were |
T12681 |
40620-40624 |
IN |
denotes |
with |
T12682 |
40625-40626 |
DT |
denotes |
a |
T12683 |
40630-40635 |
NN |
denotes |
probe |
T12684 |
40627-40628 |
CD |
denotes |
5 |
T12685 |
40628-40629 |
SYM |
denotes |
′ |
T12686 |
40636-40637 |
-LRB- |
denotes |
( |
T12687 |
40735-40741 |
NN |
denotes |
digest |
T12688 |
40637-40643 |
NNS |
denotes |
oligos |
T12689 |
40643-40645 |
, |
denotes |
, |
T12690 |
40645-40646 |
CD |
denotes |
5 |
T12691 |
40649-40673 |
NN |
denotes |
GAGATGACCCAGCCAGGATGCCTC |
T12692 |
40646-40647 |
SYM |
denotes |
′ |
T12693 |
40647-40648 |
HYPH |
denotes |
- |
T12694 |
40673-40674 |
HYPH |
denotes |
- |
T12695 |
40674-40675 |
CD |
denotes |
3 |
T12696 |
40675-40676 |
SYM |
denotes |
′ |
T12697 |
40677-40680 |
CC |
denotes |
and |
T12698 |
40681-40682 |
CD |
denotes |
5 |
T12699 |
40685-40709 |
NN |
denotes |
CTGACCACTCTCGCTCCGGTGTCC |
T12700 |
40682-40683 |
SYM |
denotes |
′ |
T12701 |
40683-40684 |
HYPH |
denotes |
- |
T12702 |
40709-40710 |
HYPH |
denotes |
- |
T12703 |
40710-40711 |
CD |
denotes |
3 |
T12704 |
40711-40712 |
SYM |
denotes |
′ |
T12705 |
40712-40713 |
: |
denotes |
; |
T12706 |
40714-40721 |
JJ |
denotes |
genomic |
T12707 |
40722-40725 |
NN |
denotes |
DNA |
T12708 |
40725-40727 |
, |
denotes |
, |
T12709 |
40727-40734 |
NN |
denotes |
HindIII |
T12710 |
40741-40742 |
-RRB- |
denotes |
) |
T12711 |
40742-40743 |
. |
denotes |
. |
T12712 |
40743-40818 |
sentence |
denotes |
The frequency of recombination in 129/Ola ES cells was approximately 1/20. |
T12713 |
40744-40747 |
DT |
denotes |
The |
T12714 |
40748-40757 |
NN |
denotes |
frequency |
T12715 |
40795-40798 |
VBD |
denotes |
was |
T12716 |
40758-40760 |
IN |
denotes |
of |
T12717 |
40761-40774 |
NN |
denotes |
recombination |
T12718 |
40775-40777 |
IN |
denotes |
in |
T12719 |
40778-40781 |
CD |
denotes |
129 |
T12720 |
40782-40785 |
NN |
denotes |
Ola |
T12721 |
40781-40782 |
HYPH |
denotes |
/ |
T12722 |
40789-40794 |
NNS |
denotes |
cells |
T12723 |
40786-40788 |
NN |
denotes |
ES |
T12724 |
40799-40812 |
RB |
denotes |
approximately |
T12725 |
40815-40817 |
CD |
denotes |
20 |
T12726 |
40813-40814 |
CD |
denotes |
1 |
T12727 |
40814-40815 |
SYM |
denotes |
/ |
T12728 |
40817-40818 |
. |
denotes |
. |
T12729 |
40818-40950 |
sentence |
denotes |
Recombinant clones were aggregated with morula stage embryos to generate chimeric founder mice that transmitted the mutant alleles. |
T12730 |
40819-40830 |
JJ |
denotes |
Recombinant |
T12731 |
40831-40837 |
NNS |
denotes |
clones |
T12732 |
40843-40853 |
VBN |
denotes |
aggregated |
T12733 |
40838-40842 |
VBD |
denotes |
were |
T12734 |
40854-40858 |
IN |
denotes |
with |
T12735 |
40859-40865 |
NN |
denotes |
morula |
T12736 |
40866-40871 |
NN |
denotes |
stage |
T12737 |
40872-40879 |
NNS |
denotes |
embryos |
T12738 |
40880-40882 |
TO |
denotes |
to |
T12739 |
40883-40891 |
VB |
denotes |
generate |
T12740 |
40892-40900 |
JJ |
denotes |
chimeric |
T12741 |
40909-40913 |
NNS |
denotes |
mice |
T12742 |
40901-40908 |
NN |
denotes |
founder |
T12743 |
40914-40918 |
WDT |
denotes |
that |
T12744 |
40919-40930 |
VBD |
denotes |
transmitted |
T12745 |
40931-40934 |
DT |
denotes |
the |
T12746 |
40942-40949 |
NNS |
denotes |
alleles |
T12747 |
40935-40941 |
NN |
denotes |
mutant |
T12748 |
40949-40950 |
. |
denotes |
. |
T12749 |
40950-41057 |
sentence |
denotes |
In all experiments performed in this study, animals were of mixed genetic background (129/Ola and C57Bl6). |
T12750 |
40951-40953 |
IN |
denotes |
In |
T12751 |
41003-41007 |
VBD |
denotes |
were |
T12752 |
40954-40957 |
DT |
denotes |
all |
T12753 |
40958-40969 |
NNS |
denotes |
experiments |
T12754 |
40970-40979 |
VBN |
denotes |
performed |
T12755 |
40980-40982 |
IN |
denotes |
in |
T12756 |
40983-40987 |
DT |
denotes |
this |
T12757 |
40988-40993 |
NN |
denotes |
study |
T12758 |
40993-40995 |
, |
denotes |
, |
T12759 |
40995-41002 |
NNS |
denotes |
animals |
T12760 |
41008-41010 |
IN |
denotes |
of |
T12761 |
41011-41016 |
JJ |
denotes |
mixed |
T12762 |
41025-41035 |
NN |
denotes |
background |
T12763 |
41017-41024 |
JJ |
denotes |
genetic |
T12764 |
41036-41037 |
-LRB- |
denotes |
( |
T12765 |
41041-41044 |
NN |
denotes |
Ola |
T12766 |
41037-41040 |
CD |
denotes |
129 |
T12767 |
41040-41041 |
HYPH |
denotes |
/ |
T12768 |
41045-41048 |
CC |
denotes |
and |
T12769 |
41049-41055 |
NN |
denotes |
C57Bl6 |
T12770 |
41055-41056 |
-RRB- |
denotes |
) |
T12771 |
41056-41057 |
. |
denotes |
. |
T12772 |
41057-41223 |
sentence |
denotes |
Thy1spGFP transgenic mice were generated in analogy to De Paola et al. [25], and for these experiments a strain of mice with early embryonic expression was selected. |
T12773 |
41058-41067 |
NN |
denotes |
Thy1spGFP |
T12774 |
41079-41083 |
NNS |
denotes |
mice |
T12775 |
41068-41078 |
JJ |
denotes |
transgenic |
T12776 |
41089-41098 |
VBN |
denotes |
generated |
T12777 |
41084-41088 |
VBD |
denotes |
were |
T12778 |
41099-41101 |
IN |
denotes |
in |
T12779 |
41102-41109 |
NN |
denotes |
analogy |
T12780 |
41110-41112 |
IN |
denotes |
to |
T12781 |
41113-41115 |
NNP |
denotes |
De |
T12782 |
41116-41121 |
NNP |
denotes |
Paola |
T12783 |
41122-41124 |
FW |
denotes |
et |
T12784 |
41125-41128 |
FW |
denotes |
al. |
T12785 |
41129-41130 |
-LRB- |
denotes |
[ |
T12786 |
41130-41132 |
CD |
denotes |
25 |
T12787 |
41132-41133 |
-RRB- |
denotes |
] |
T12788 |
41133-41135 |
, |
denotes |
, |
T12789 |
41135-41138 |
CC |
denotes |
and |
T12790 |
41139-41142 |
IN |
denotes |
for |
T12791 |
41214-41222 |
VBN |
denotes |
selected |
T12792 |
41143-41148 |
DT |
denotes |
these |
T12793 |
41149-41160 |
NNS |
denotes |
experiments |
T12794 |
41161-41162 |
DT |
denotes |
a |
T12795 |
41163-41169 |
NN |
denotes |
strain |
T12796 |
41170-41172 |
IN |
denotes |
of |
T12797 |
41173-41177 |
NNS |
denotes |
mice |
T12798 |
41178-41182 |
IN |
denotes |
with |
T12799 |
41183-41188 |
JJ |
denotes |
early |
T12800 |
41199-41209 |
NN |
denotes |
expression |
T12801 |
41189-41198 |
JJ |
denotes |
embryonic |
T12802 |
41210-41213 |
VBD |
denotes |
was |
T12803 |
41222-41223 |
. |
denotes |
. |
T12804 |
41223-41373 |
sentence |
denotes |
Isl1Cre and Hb9Cre mouse strains have been described [33,43] and Bax+/− animals were from Jackson Laboratory (Bar Harbor, Maine, United States) [27]. |
T12805 |
41224-41231 |
NN |
denotes |
Isl1Cre |
T12806 |
41249-41256 |
NNS |
denotes |
strains |
T12807 |
41232-41235 |
CC |
denotes |
and |
T12808 |
41236-41242 |
NN |
denotes |
Hb9Cre |
T12809 |
41243-41248 |
NN |
denotes |
mouse |
T12810 |
41267-41276 |
VBN |
denotes |
described |
T12811 |
41257-41261 |
VBP |
denotes |
have |
T12812 |
41262-41266 |
VBN |
denotes |
been |
T12813 |
41277-41278 |
-LRB- |
denotes |
[ |
T12814 |
41281-41283 |
CD |
denotes |
43 |
T12815 |
41278-41280 |
CD |
denotes |
33 |
T12816 |
41280-41281 |
, |
denotes |
, |
T12817 |
41283-41284 |
-RRB- |
denotes |
] |
T12818 |
41285-41288 |
CC |
denotes |
and |
T12819 |
41289-41292 |
NN |
denotes |
Bax |
T12820 |
41296-41303 |
NNS |
denotes |
animals |
T12821 |
41292-41293 |
SYM |
denotes |
+ |
T12822 |
41293-41294 |
HYPH |
denotes |
/ |
T12823 |
41294-41295 |
SYM |
denotes |
− |
T12824 |
41304-41308 |
VBD |
denotes |
were |
T12825 |
41309-41313 |
IN |
denotes |
from |
T12826 |
41314-41321 |
NNP |
denotes |
Jackson |
T12827 |
41322-41332 |
NNP |
denotes |
Laboratory |
T12828 |
41333-41334 |
-LRB- |
denotes |
( |
T12829 |
41338-41344 |
NNP |
denotes |
Harbor |
T12830 |
41334-41337 |
NNP |
denotes |
Bar |
T12831 |
41344-41346 |
, |
denotes |
, |
T12832 |
41346-41351 |
NNP |
denotes |
Maine |
T12833 |
41351-41353 |
, |
denotes |
, |
T12834 |
41353-41359 |
NNP |
denotes |
United |
T12835 |
41360-41366 |
NNP |
denotes |
States |
T12836 |
41366-41367 |
-RRB- |
denotes |
) |
T12837 |
41368-41369 |
-LRB- |
denotes |
[ |
T12838 |
41369-41371 |
CD |
denotes |
27 |
T12839 |
41371-41372 |
-RRB- |
denotes |
] |
T12840 |
41372-41373 |
. |
denotes |
. |
T12841 |
41373-41508 |
sentence |
denotes |
Timed pregnancies were set up to generate embryos of different developmental stages with all genotypes described throughout the study. |
T12842 |
41374-41379 |
JJ |
denotes |
Timed |
T12843 |
41380-41391 |
NNS |
denotes |
pregnancies |
T12844 |
41397-41400 |
VBN |
denotes |
set |
T12845 |
41392-41396 |
VBD |
denotes |
were |
T12846 |
41401-41403 |
RP |
denotes |
up |
T12847 |
41404-41406 |
TO |
denotes |
to |
T12848 |
41407-41415 |
VB |
denotes |
generate |
T12849 |
41416-41423 |
NNS |
denotes |
embryos |
T12850 |
41424-41426 |
IN |
denotes |
of |
T12851 |
41427-41436 |
JJ |
denotes |
different |
T12852 |
41451-41457 |
NNS |
denotes |
stages |
T12853 |
41437-41450 |
JJ |
denotes |
developmental |
T12854 |
41458-41462 |
IN |
denotes |
with |
T12855 |
41477-41486 |
VBN |
denotes |
described |
T12856 |
41463-41466 |
DT |
denotes |
all |
T12857 |
41467-41476 |
NNS |
denotes |
genotypes |
T12858 |
41487-41497 |
IN |
denotes |
throughout |
T12859 |
41498-41501 |
DT |
denotes |
the |
T12860 |
41502-41507 |
NN |
denotes |
study |
T12861 |
41507-41508 |
. |
denotes |
. |
T13099 |
41510-41525 |
JJ |
denotes |
Transcriptional |
T13100 |
41526-41541 |
NN |
denotes |
transactivation |
T13101 |
41542-41548 |
NNS |
denotes |
assays |
T13102 |
41548-41745 |
sentence |
denotes |
The following plasmids were used for transcriptional transactivation assays: pRc/RSV (Invitrogen, Carlsbad, California, United States), pRc/RSV-Er81, pRc/RSV-EWS-Pea3, pTP-5xETS, and pTP-5xETSmut. |
T13103 |
41549-41552 |
DT |
denotes |
The |
T13104 |
41563-41571 |
NNS |
denotes |
plasmids |
T13105 |
41553-41562 |
VBG |
denotes |
following |
T13106 |
41577-41581 |
VBN |
denotes |
used |
T13107 |
41572-41576 |
VBD |
denotes |
were |
T13108 |
41582-41585 |
IN |
denotes |
for |
T13109 |
41586-41601 |
JJ |
denotes |
transcriptional |
T13110 |
41618-41624 |
NNS |
denotes |
assays |
T13111 |
41602-41617 |
NN |
denotes |
transactivation |
T13112 |
41624-41626 |
: |
denotes |
: |
T13113 |
41626-41629 |
NN |
denotes |
pRc |
T13114 |
41630-41633 |
NN |
denotes |
RSV |
T13115 |
41629-41630 |
HYPH |
denotes |
/ |
T13116 |
41634-41635 |
-LRB- |
denotes |
( |
T13117 |
41635-41645 |
NNP |
denotes |
Invitrogen |
T13118 |
41645-41647 |
, |
denotes |
, |
T13119 |
41647-41655 |
NNP |
denotes |
Carlsbad |
T13120 |
41655-41657 |
, |
denotes |
, |
T13121 |
41657-41667 |
NNP |
denotes |
California |
T13122 |
41667-41669 |
, |
denotes |
, |
T13123 |
41669-41675 |
NNP |
denotes |
United |
T13124 |
41676-41682 |
NNP |
denotes |
States |
T13125 |
41682-41683 |
-RRB- |
denotes |
) |
T13126 |
41683-41685 |
, |
denotes |
, |
T13127 |
41685-41688 |
NN |
denotes |
pRc |
T13128 |
41689-41692 |
NN |
denotes |
RSV |
T13129 |
41688-41689 |
HYPH |
denotes |
/ |
T13130 |
41693-41697 |
NN |
denotes |
Er81 |
T13131 |
41692-41693 |
HYPH |
denotes |
- |
T13132 |
41697-41699 |
, |
denotes |
, |
T13133 |
41699-41702 |
NN |
denotes |
pRc |
T13134 |
41703-41706 |
NN |
denotes |
RSV |
T13135 |
41702-41703 |
HYPH |
denotes |
/ |
T13136 |
41711-41715 |
NN |
denotes |
Pea3 |
T13137 |
41706-41707 |
HYPH |
denotes |
- |
T13138 |
41707-41710 |
NN |
denotes |
EWS |
T13139 |
41710-41711 |
HYPH |
denotes |
- |
T13140 |
41715-41717 |
, |
denotes |
, |
T13141 |
41717-41720 |
NN |
denotes |
pTP |
T13142 |
41721-41726 |
NN |
denotes |
5xETS |
T13143 |
41720-41721 |
HYPH |
denotes |
- |
T13144 |
41726-41728 |
, |
denotes |
, |
T13145 |
41728-41731 |
CC |
denotes |
and |
T13146 |
41732-41735 |
NN |
denotes |
pTP |
T13147 |
41736-41744 |
NN |
denotes |
5xETSmut |
T13148 |
41735-41736 |
HYPH |
denotes |
- |
T13149 |
41744-41745 |
. |
denotes |
. |
T13150 |
41745-41880 |
sentence |
denotes |
pRc/RSV-Er81 and pRc/RSV-EWS-Pea3 were obtained by insertion of the cDNAs for Er81 or EWS-Pea3 (gift from J. A. Hassell) into pRc/RSV. |
T13151 |
41746-41749 |
NN |
denotes |
pRc |
T13152 |
41750-41753 |
NN |
denotes |
RSV |
T13153 |
41749-41750 |
HYPH |
denotes |
/ |
T13154 |
41754-41758 |
NN |
denotes |
Er81 |
T13155 |
41753-41754 |
HYPH |
denotes |
- |
T13156 |
41785-41793 |
VBN |
denotes |
obtained |
T13157 |
41759-41762 |
CC |
denotes |
and |
T13158 |
41763-41766 |
NN |
denotes |
pRc |
T13159 |
41767-41770 |
NN |
denotes |
RSV |
T13160 |
41766-41767 |
HYPH |
denotes |
/ |
T13161 |
41775-41779 |
NN |
denotes |
Pea3 |
T13162 |
41770-41771 |
HYPH |
denotes |
- |
T13163 |
41771-41774 |
NN |
denotes |
EWS |
T13164 |
41774-41775 |
HYPH |
denotes |
- |
T13165 |
41780-41784 |
VBD |
denotes |
were |
T13166 |
41794-41796 |
IN |
denotes |
by |
T13167 |
41797-41806 |
NN |
denotes |
insertion |
T13168 |
41807-41809 |
IN |
denotes |
of |
T13169 |
41810-41813 |
DT |
denotes |
the |
T13170 |
41814-41819 |
NNS |
denotes |
cDNAs |
T13171 |
41820-41823 |
IN |
denotes |
for |
T13172 |
41824-41828 |
NN |
denotes |
Er81 |
T13173 |
41829-41831 |
CC |
denotes |
or |
T13174 |
41832-41835 |
NN |
denotes |
EWS |
T13175 |
41836-41840 |
NN |
denotes |
Pea3 |
T13176 |
41835-41836 |
HYPH |
denotes |
- |
T13177 |
41841-41842 |
-LRB- |
denotes |
( |
T13178 |
41842-41846 |
NN |
denotes |
gift |
T13179 |
41847-41851 |
IN |
denotes |
from |
T13180 |
41852-41854 |
NNP |
denotes |
J. |
T13181 |
41858-41865 |
NNP |
denotes |
Hassell |
T13182 |
41855-41857 |
NNP |
denotes |
A. |
T13183 |
41865-41866 |
-RRB- |
denotes |
) |
T13184 |
41867-41871 |
IN |
denotes |
into |
T13185 |
41872-41875 |
NN |
denotes |
pRc |
T13186 |
41876-41879 |
NN |
denotes |
RSV |
T13187 |
41875-41876 |
HYPH |
denotes |
/ |
T13188 |
41879-41880 |
. |
denotes |
. |
T13189 |
41880-42055 |
sentence |
denotes |
pTP-5xETS was constructed by inserting a cassette of five repetitive copies of high-affinity Pea3 binding sites (5′- GCCGGAAGC-3′) [18,19] into a modified version of pTK-Luc. |
T13190 |
41881-41884 |
NN |
denotes |
pTP |
T13191 |
41885-41890 |
NN |
denotes |
5xETS |
T13192 |
41884-41885 |
HYPH |
denotes |
- |
T13193 |
41895-41906 |
VBN |
denotes |
constructed |
T13194 |
41891-41894 |
VBD |
denotes |
was |
T13195 |
41907-41909 |
IN |
denotes |
by |
T13196 |
41910-41919 |
VBG |
denotes |
inserting |
T13197 |
41920-41921 |
DT |
denotes |
a |
T13198 |
41922-41930 |
NN |
denotes |
cassette |
T13199 |
41931-41933 |
IN |
denotes |
of |
T13200 |
41934-41938 |
CD |
denotes |
five |
T13201 |
41950-41956 |
NNS |
denotes |
copies |
T13202 |
41939-41949 |
JJ |
denotes |
repetitive |
T13203 |
41957-41959 |
IN |
denotes |
of |
T13204 |
41960-41964 |
JJ |
denotes |
high |
T13205 |
41965-41973 |
NN |
denotes |
affinity |
T13206 |
41964-41965 |
HYPH |
denotes |
- |
T13207 |
41987-41992 |
NNS |
denotes |
sites |
T13208 |
41974-41978 |
NN |
denotes |
Pea3 |
T13209 |
41979-41986 |
NN |
denotes |
binding |
T13210 |
41993-41994 |
-LRB- |
denotes |
( |
T13211 |
42008-42009 |
CD |
denotes |
3 |
T13212 |
41994-41995 |
CD |
denotes |
5 |
T13213 |
41995-41996 |
SYM |
denotes |
′ |
T13214 |
41996-41997 |
HYPH |
denotes |
- |
T13215 |
41998-42007 |
NN |
denotes |
GCCGGAAGC |
T13216 |
42007-42008 |
HYPH |
denotes |
- |
T13217 |
42009-42010 |
SYM |
denotes |
′ |
T13218 |
42010-42011 |
-RRB- |
denotes |
) |
T13219 |
42012-42013 |
-LRB- |
denotes |
[ |
T13220 |
42016-42018 |
CD |
denotes |
19 |
T13221 |
42013-42015 |
CD |
denotes |
18 |
T13222 |
42015-42016 |
, |
denotes |
, |
T13223 |
42018-42019 |
-RRB- |
denotes |
] |
T13224 |
42020-42024 |
IN |
denotes |
into |
T13225 |
42025-42026 |
DT |
denotes |
a |
T13226 |
42036-42043 |
NN |
denotes |
version |
T13227 |
42027-42035 |
VBN |
denotes |
modified |
T13228 |
42044-42046 |
IN |
denotes |
of |
T13229 |
42047-42050 |
NN |
denotes |
pTK |
T13230 |
42051-42054 |
NN |
denotes |
Luc |
T13231 |
42050-42051 |
HYPH |
denotes |
- |
T13232 |
42054-42055 |
. |
denotes |
. |
T13233 |
42055-42172 |
sentence |
denotes |
pTP-5xETSmut was generated as pTP-5xETS but using a mutated complement of the Pea3 binding sites (5′- GCCTATGGC-3′). |
T13234 |
42056-42059 |
NN |
denotes |
pTP |
T13235 |
42060-42068 |
NN |
denotes |
5xETSmut |
T13236 |
42059-42060 |
HYPH |
denotes |
- |
T13237 |
42073-42082 |
VBN |
denotes |
generated |
T13238 |
42069-42072 |
VBD |
denotes |
was |
T13239 |
42083-42085 |
IN |
denotes |
as |
T13240 |
42086-42089 |
NN |
denotes |
pTP |
T13241 |
42090-42095 |
NN |
denotes |
5xETS |
T13242 |
42089-42090 |
HYPH |
denotes |
- |
T13243 |
42096-42099 |
CC |
denotes |
but |
T13244 |
42100-42105 |
VBG |
denotes |
using |
T13245 |
42106-42107 |
DT |
denotes |
a |
T13246 |
42116-42126 |
NN |
denotes |
complement |
T13247 |
42108-42115 |
VBN |
denotes |
mutated |
T13248 |
42127-42129 |
IN |
denotes |
of |
T13249 |
42130-42133 |
DT |
denotes |
the |
T13250 |
42147-42152 |
NNS |
denotes |
sites |
T13251 |
42134-42138 |
NN |
denotes |
Pea3 |
T13252 |
42139-42146 |
NN |
denotes |
binding |
T13253 |
42153-42154 |
-LRB- |
denotes |
( |
T13254 |
42168-42169 |
CD |
denotes |
3 |
T13255 |
42154-42155 |
CD |
denotes |
5 |
T13256 |
42155-42156 |
SYM |
denotes |
′ |
T13257 |
42156-42157 |
HYPH |
denotes |
- |
T13258 |
42158-42167 |
NN |
denotes |
GCCTATGGC |
T13259 |
42167-42168 |
HYPH |
denotes |
- |
T13260 |
42169-42170 |
SYM |
denotes |
′ |
T13261 |
42170-42171 |
-RRB- |
denotes |
) |
T13262 |
42171-42172 |
. |
denotes |
. |
T13263 |
42172-42281 |
sentence |
denotes |
A control plasmid to normalize for transfection efficiency (placZ) and pTK-Luc were a gift from D. Kressler. |
T13264 |
42173-42174 |
DT |
denotes |
A |
T13265 |
42183-42190 |
NN |
denotes |
plasmid |
T13266 |
42175-42182 |
NN |
denotes |
control |
T13267 |
42252-42256 |
VBD |
denotes |
were |
T13268 |
42191-42193 |
TO |
denotes |
to |
T13269 |
42194-42203 |
VB |
denotes |
normalize |
T13270 |
42204-42207 |
IN |
denotes |
for |
T13271 |
42208-42220 |
NN |
denotes |
transfection |
T13272 |
42221-42231 |
NN |
denotes |
efficiency |
T13273 |
42232-42233 |
-LRB- |
denotes |
( |
T13274 |
42233-42238 |
NN |
denotes |
placZ |
T13275 |
42238-42239 |
-RRB- |
denotes |
) |
T13276 |
42240-42243 |
CC |
denotes |
and |
T13277 |
42244-42247 |
NN |
denotes |
pTK |
T13278 |
42248-42251 |
NN |
denotes |
Luc |
T13279 |
42247-42248 |
HYPH |
denotes |
- |
T13280 |
42257-42258 |
DT |
denotes |
a |
T13281 |
42259-42263 |
NN |
denotes |
gift |
T13282 |
42264-42268 |
IN |
denotes |
from |
T13283 |
42269-42271 |
NNP |
denotes |
D. |
T13284 |
42272-42280 |
NNP |
denotes |
Kressler |
T13285 |
42280-42281 |
. |
denotes |
. |
T13286 |
42281-42496 |
sentence |
denotes |
COS-7 cells were co-transfected with 1–1.2 μg of total DNA including one of the effector plasmids pRc/RSV-empty, pRc/RSV-Er81, or pRc/RSV-EWS-Pea3; one of the reporter plasmids pTP-5xETS or pTP-5xETSmut; and placZ. |
T13287 |
42282-42285 |
NN |
denotes |
COS |
T13288 |
42288-42293 |
NNS |
denotes |
cells |
T13289 |
42285-42286 |
HYPH |
denotes |
- |
T13290 |
42286-42287 |
CD |
denotes |
7 |
T13291 |
42299-42313 |
VBN |
denotes |
co-transfected |
T13292 |
42294-42298 |
VBD |
denotes |
were |
T13293 |
42314-42318 |
IN |
denotes |
with |
T13294 |
42319-42320 |
CD |
denotes |
1 |
T13295 |
42321-42324 |
CD |
denotes |
1.2 |
T13296 |
42320-42321 |
SYM |
denotes |
– |
T13297 |
42325-42327 |
NN |
denotes |
μg |
T13298 |
42328-42330 |
IN |
denotes |
of |
T13299 |
42331-42336 |
JJ |
denotes |
total |
T13300 |
42337-42340 |
NN |
denotes |
DNA |
T13301 |
42341-42350 |
VBG |
denotes |
including |
T13302 |
42351-42354 |
CD |
denotes |
one |
T13303 |
42355-42357 |
IN |
denotes |
of |
T13304 |
42358-42361 |
DT |
denotes |
the |
T13305 |
42371-42379 |
NNS |
denotes |
plasmids |
T13306 |
42362-42370 |
NN |
denotes |
effector |
T13307 |
42380-42383 |
NN |
denotes |
pRc |
T13308 |
42384-42387 |
NN |
denotes |
RSV |
T13309 |
42383-42384 |
HYPH |
denotes |
/ |
T13310 |
42387-42388 |
HYPH |
denotes |
- |
T13311 |
42388-42393 |
JJ |
denotes |
empty |
T13312 |
42393-42395 |
, |
denotes |
, |
T13313 |
42395-42398 |
NN |
denotes |
pRc |
T13314 |
42399-42402 |
NN |
denotes |
RSV |
T13315 |
42398-42399 |
HYPH |
denotes |
/ |
T13316 |
42403-42407 |
NN |
denotes |
Er81 |
T13317 |
42402-42403 |
HYPH |
denotes |
- |
T13318 |
42407-42409 |
, |
denotes |
, |
T13319 |
42409-42411 |
CC |
denotes |
or |
T13320 |
42412-42415 |
NN |
denotes |
pRc |
T13321 |
42416-42419 |
NN |
denotes |
RSV |
T13322 |
42415-42416 |
HYPH |
denotes |
/ |
T13323 |
42424-42428 |
NN |
denotes |
Pea3 |
T13324 |
42419-42420 |
HYPH |
denotes |
- |
T13325 |
42420-42423 |
NN |
denotes |
EWS |
T13326 |
42423-42424 |
HYPH |
denotes |
- |
T13327 |
42428-42429 |
: |
denotes |
; |
T13328 |
42430-42433 |
CD |
denotes |
one |
T13329 |
42434-42436 |
IN |
denotes |
of |
T13330 |
42437-42440 |
DT |
denotes |
the |
T13331 |
42450-42458 |
NNS |
denotes |
plasmids |
T13332 |
42441-42449 |
NN |
denotes |
reporter |
T13333 |
42459-42462 |
NN |
denotes |
pTP |
T13334 |
42463-42468 |
NN |
denotes |
5xETS |
T13335 |
42462-42463 |
HYPH |
denotes |
- |
T13336 |
42469-42471 |
CC |
denotes |
or |
T13337 |
42472-42475 |
NN |
denotes |
pTP |
T13338 |
42476-42484 |
NN |
denotes |
5xETSmut |
T13339 |
42475-42476 |
HYPH |
denotes |
- |
T13340 |
42484-42485 |
: |
denotes |
; |
T13341 |
42486-42489 |
CC |
denotes |
and |
T13342 |
42490-42495 |
NN |
denotes |
placZ |
T13343 |
42495-42496 |
. |
denotes |
. |
T13344 |
42496-42625 |
sentence |
denotes |
Cells were harvested after 25 h and processed for assays to determine luciferase and LacZ activity as described previously [44]. |
T13345 |
42497-42502 |
NNS |
denotes |
Cells |
T13346 |
42508-42517 |
VBN |
denotes |
harvested |
T13347 |
42503-42507 |
VBD |
denotes |
were |
T13348 |
42518-42523 |
IN |
denotes |
after |
T13349 |
42524-42526 |
CD |
denotes |
25 |
T13350 |
42527-42528 |
NN |
denotes |
h |
T13351 |
42529-42532 |
CC |
denotes |
and |
T13352 |
42533-42542 |
VBN |
denotes |
processed |
T13353 |
42543-42546 |
IN |
denotes |
for |
T13354 |
42547-42553 |
NNS |
denotes |
assays |
T13355 |
42554-42556 |
TO |
denotes |
to |
T13356 |
42557-42566 |
VB |
denotes |
determine |
T13357 |
42567-42577 |
NN |
denotes |
luciferase |
T13358 |
42587-42595 |
NN |
denotes |
activity |
T13359 |
42578-42581 |
CC |
denotes |
and |
T13360 |
42582-42586 |
NN |
denotes |
LacZ |
T13361 |
42596-42598 |
IN |
denotes |
as |
T13362 |
42599-42608 |
VBN |
denotes |
described |
T13363 |
42609-42619 |
RB |
denotes |
previously |
T13364 |
42620-42621 |
-LRB- |
denotes |
[ |
T13365 |
42621-42623 |
CD |
denotes |
44 |
T13366 |
42623-42624 |
-RRB- |
denotes |
] |
T13367 |
42624-42625 |
. |
denotes |
. |
T13368 |
42625-42708 |
sentence |
denotes |
Luciferase values normalized to LacZ activity are referred to as luciferase units. |
T13369 |
42626-42636 |
NN |
denotes |
Luciferase |
T13370 |
42637-42643 |
NNS |
denotes |
values |
T13371 |
42676-42684 |
VBN |
denotes |
referred |
T13372 |
42644-42654 |
VBN |
denotes |
normalized |
T13373 |
42655-42657 |
IN |
denotes |
to |
T13374 |
42658-42662 |
NN |
denotes |
LacZ |
T13375 |
42663-42671 |
NN |
denotes |
activity |
T13376 |
42672-42675 |
VBP |
denotes |
are |
T13377 |
42685-42687 |
IN |
denotes |
to |
T13378 |
42688-42690 |
IN |
denotes |
as |
T13379 |
42691-42701 |
NN |
denotes |
luciferase |
T13380 |
42702-42707 |
NNS |
denotes |
units |
T13381 |
42707-42708 |
. |
denotes |
. |
T13917 |
42710-42712 |
FW |
denotes |
In |
T13918 |
42713-42717 |
FW |
denotes |
situ |
T13919 |
42718-42731 |
NN |
denotes |
hybridization |
T13920 |
42732-42735 |
CC |
denotes |
and |
T13921 |
42736-42756 |
NN |
denotes |
immunohistochemistry |
T13922 |
42756-42939 |
sentence |
denotes |
For in situ hybridization analysis, cryostat sections were hybridized using digoxigenin-labeled probes [45] directed against mouse TrkA or TrkB, or rat TrkC (gift from L. F. Parada). |
T13923 |
42757-42760 |
IN |
denotes |
For |
T13924 |
42816-42826 |
VBN |
denotes |
hybridized |
T13925 |
42761-42763 |
FW |
denotes |
in |
T13926 |
42764-42768 |
FW |
denotes |
situ |
T13927 |
42783-42791 |
NN |
denotes |
analysis |
T13928 |
42769-42782 |
NN |
denotes |
hybridization |
T13929 |
42791-42793 |
, |
denotes |
, |
T13930 |
42793-42801 |
NN |
denotes |
cryostat |
T13931 |
42802-42810 |
NNS |
denotes |
sections |
T13932 |
42811-42815 |
VBD |
denotes |
were |
T13933 |
42827-42832 |
VBG |
denotes |
using |
T13934 |
42833-42844 |
NN |
denotes |
digoxigenin |
T13935 |
42845-42852 |
VBN |
denotes |
labeled |
T13936 |
42844-42845 |
HYPH |
denotes |
- |
T13937 |
42853-42859 |
NNS |
denotes |
probes |
T13938 |
42860-42861 |
-LRB- |
denotes |
[ |
T13939 |
42861-42863 |
CD |
denotes |
45 |
T13940 |
42863-42864 |
-RRB- |
denotes |
] |
T13941 |
42865-42873 |
VBN |
denotes |
directed |
T13942 |
42874-42881 |
IN |
denotes |
against |
T13943 |
42882-42887 |
NN |
denotes |
mouse |
T13944 |
42888-42892 |
NN |
denotes |
TrkA |
T13945 |
42893-42895 |
CC |
denotes |
or |
T13946 |
42896-42900 |
NN |
denotes |
TrkB |
T13947 |
42900-42902 |
, |
denotes |
, |
T13948 |
42902-42904 |
CC |
denotes |
or |
T13949 |
42905-42908 |
NN |
denotes |
rat |
T13950 |
42909-42913 |
NN |
denotes |
TrkC |
T13951 |
42914-42915 |
-LRB- |
denotes |
( |
T13952 |
42915-42919 |
NN |
denotes |
gift |
T13953 |
42920-42924 |
IN |
denotes |
from |
T13954 |
42925-42927 |
NNP |
denotes |
L. |
T13955 |
42931-42937 |
NNP |
denotes |
Parada |
T13956 |
42928-42930 |
NNP |
denotes |
F. |
T13957 |
42937-42938 |
-RRB- |
denotes |
) |
T13958 |
42938-42939 |
. |
denotes |
. |
T13959 |
42939-43769 |
sentence |
denotes |
Antibodies used in this study were as follows: rabbit anti-Er81 [14], rabbit anti-Pea3 [14], rabbit anti-PV [14], rabbit anti-eGFP (Molecular Probes, Eugene, Oregon, United States), rabbit anti-Calbindin, rabbit anti-Calretinin (Swant, Bellinzona, Switzerland), rabbit anti-CGRP (Chemicon, Temecula, California, United States), rabbit anti-vGlut1 (Synaptic Systems, Goettingen, Germany), rabbit anti-Brn3a (gift from E. Turner), rabbit anti-TrkA and -p75 (gift from L. F. Reichardt), rabbit anti-Runx3 (Kramer and Arber, unpublished reagent), rabbit anti-Rhodamine (Molecular Probes), mouse anti-neurofilament (American Type Culture Collection, Manassas, Virginia, United States), sheep anti-eGFP (Biogenesis, Poole, United Kingdom), goat anti-LacZ [14], goat anti-TrkC (gift from L. F. Reichardt), and guinea pig anti-Isl1 [14]. |
T13960 |
42940-42950 |
NNS |
denotes |
Antibodies |
T13961 |
42970-42974 |
VBD |
denotes |
were |
T13962 |
42951-42955 |
VBN |
denotes |
used |
T13963 |
42956-42958 |
IN |
denotes |
in |
T13964 |
42959-42963 |
DT |
denotes |
this |
T13965 |
42964-42969 |
NN |
denotes |
study |
T13966 |
42975-42977 |
IN |
denotes |
as |
T13967 |
42978-42985 |
VBZ |
denotes |
follows |
T13968 |
42985-42987 |
: |
denotes |
: |
T13969 |
42987-42993 |
NN |
denotes |
rabbit |
T13970 |
42994-43003 |
NN |
denotes |
anti-Er81 |
T13971 |
43004-43005 |
-LRB- |
denotes |
[ |
T13972 |
43005-43007 |
CD |
denotes |
14 |
T13973 |
43007-43008 |
-RRB- |
denotes |
] |
T13974 |
43008-43010 |
, |
denotes |
, |
T13975 |
43010-43016 |
NN |
denotes |
rabbit |
T13976 |
43017-43026 |
JJ |
denotes |
anti-Pea3 |
T13977 |
43027-43028 |
-LRB- |
denotes |
[ |
T13978 |
43028-43030 |
CD |
denotes |
14 |
T13979 |
43030-43031 |
-RRB- |
denotes |
] |
T13980 |
43031-43033 |
, |
denotes |
, |
T13981 |
43033-43039 |
NN |
denotes |
rabbit |
T13982 |
43040-43047 |
JJ |
denotes |
anti-PV |
T13983 |
43048-43049 |
-LRB- |
denotes |
[ |
T13984 |
43049-43051 |
CD |
denotes |
14 |
T13985 |
43051-43052 |
-RRB- |
denotes |
] |
T13986 |
43052-43054 |
, |
denotes |
, |
T13987 |
43054-43060 |
NN |
denotes |
rabbit |
T13988 |
43061-43070 |
JJ |
denotes |
anti-eGFP |
T13989 |
43071-43072 |
-LRB- |
denotes |
( |
T13990 |
43082-43088 |
NNP |
denotes |
Probes |
T13991 |
43072-43081 |
NNP |
denotes |
Molecular |
T13992 |
43088-43090 |
, |
denotes |
, |
T13993 |
43090-43096 |
NNP |
denotes |
Eugene |
T13994 |
43096-43098 |
, |
denotes |
, |
T13995 |
43098-43104 |
NNP |
denotes |
Oregon |
T13996 |
43104-43106 |
, |
denotes |
, |
T13997 |
43106-43112 |
NNP |
denotes |
United |
T13998 |
43113-43119 |
NNP |
denotes |
States |
T13999 |
43119-43120 |
-RRB- |
denotes |
) |
T14000 |
43120-43122 |
, |
denotes |
, |
T14001 |
43122-43128 |
NN |
denotes |
rabbit |
T14002 |
43129-43143 |
JJ |
denotes |
anti-Calbindin |
T14003 |
43143-43145 |
, |
denotes |
, |
T14004 |
43145-43151 |
NN |
denotes |
rabbit |
T14005 |
43152-43167 |
JJ |
denotes |
anti-Calretinin |
T14006 |
43168-43169 |
-LRB- |
denotes |
( |
T14007 |
43169-43174 |
NNP |
denotes |
Swant |
T14008 |
43174-43176 |
, |
denotes |
, |
T14009 |
43176-43186 |
NNP |
denotes |
Bellinzona |
T14010 |
43186-43188 |
, |
denotes |
, |
T14011 |
43188-43199 |
NNP |
denotes |
Switzerland |
T14012 |
43199-43200 |
-RRB- |
denotes |
) |
T14013 |
43200-43202 |
, |
denotes |
, |
T14014 |
43202-43208 |
NN |
denotes |
rabbit |
T14015 |
43209-43218 |
JJ |
denotes |
anti-CGRP |
T14016 |
43219-43220 |
-LRB- |
denotes |
( |
T14017 |
43220-43228 |
NNP |
denotes |
Chemicon |
T14018 |
43228-43230 |
, |
denotes |
, |
T14019 |
43230-43238 |
NNP |
denotes |
Temecula |
T14020 |
43238-43240 |
, |
denotes |
, |
T14021 |
43240-43250 |
NNP |
denotes |
California |
T14022 |
43250-43252 |
, |
denotes |
, |
T14023 |
43252-43258 |
NNP |
denotes |
United |
T14024 |
43259-43265 |
NNP |
denotes |
States |
T14025 |
43265-43266 |
-RRB- |
denotes |
) |
T14026 |
43266-43268 |
, |
denotes |
, |
T14027 |
43268-43274 |
NN |
denotes |
rabbit |
T14028 |
43275-43286 |
JJ |
denotes |
anti-vGlut1 |
T14029 |
43287-43288 |
-LRB- |
denotes |
( |
T14030 |
43297-43304 |
NNP |
denotes |
Systems |
T14031 |
43288-43296 |
NN |
denotes |
Synaptic |
T14032 |
43304-43306 |
, |
denotes |
, |
T14033 |
43306-43316 |
NNP |
denotes |
Goettingen |
T14034 |
43316-43318 |
, |
denotes |
, |
T14035 |
43318-43325 |
NNP |
denotes |
Germany |
T14036 |
43325-43326 |
-RRB- |
denotes |
) |
T14037 |
43326-43328 |
, |
denotes |
, |
T14038 |
43328-43334 |
NN |
denotes |
rabbit |
T14039 |
43335-43345 |
JJ |
denotes |
anti-Brn3a |
T14040 |
43346-43347 |
-LRB- |
denotes |
( |
T14041 |
43347-43351 |
NN |
denotes |
gift |
T14042 |
43352-43356 |
IN |
denotes |
from |
T14043 |
43357-43359 |
NNP |
denotes |
E. |
T14044 |
43360-43366 |
NNP |
denotes |
Turner |
T14045 |
43366-43367 |
-RRB- |
denotes |
) |
T14046 |
43367-43369 |
, |
denotes |
, |
T14047 |
43369-43375 |
NN |
denotes |
rabbit |
T14048 |
43391-43394 |
NN |
denotes |
p75 |
T14049 |
43376-43385 |
JJ |
denotes |
anti-TrkA |
T14050 |
43386-43389 |
CC |
denotes |
and |
T14051 |
43390-43391 |
HYPH |
denotes |
- |
T14052 |
43395-43396 |
-LRB- |
denotes |
( |
T14053 |
43396-43400 |
NN |
denotes |
gift |
T14054 |
43401-43405 |
IN |
denotes |
from |
T14055 |
43406-43408 |
NNP |
denotes |
L. |
T14056 |
43412-43421 |
NNP |
denotes |
Reichardt |
T14057 |
43409-43411 |
NNP |
denotes |
F. |
T14058 |
43421-43422 |
-RRB- |
denotes |
) |
T14059 |
43422-43424 |
, |
denotes |
, |
T14060 |
43424-43430 |
NN |
denotes |
rabbit |
T14061 |
43431-43441 |
JJ |
denotes |
anti-Runx3 |
T14062 |
43442-43443 |
-LRB- |
denotes |
( |
T14063 |
43443-43449 |
NNP |
denotes |
Kramer |
T14064 |
43450-43453 |
CC |
denotes |
and |
T14065 |
43454-43459 |
NNP |
denotes |
Arber |
T14066 |
43459-43461 |
, |
denotes |
, |
T14067 |
43461-43472 |
JJ |
denotes |
unpublished |
T14068 |
43473-43480 |
NN |
denotes |
reagent |
T14069 |
43480-43481 |
-RRB- |
denotes |
) |
T14070 |
43481-43483 |
, |
denotes |
, |
T14071 |
43483-43489 |
NN |
denotes |
rabbit |
T14072 |
43490-43504 |
JJ |
denotes |
anti-Rhodamine |
T14073 |
43505-43506 |
-LRB- |
denotes |
( |
T14074 |
43516-43522 |
NNP |
denotes |
Probes |
T14075 |
43506-43515 |
NNP |
denotes |
Molecular |
T14076 |
43522-43523 |
-RRB- |
denotes |
) |
T14077 |
43523-43525 |
, |
denotes |
, |
T14078 |
43525-43530 |
NN |
denotes |
mouse |
T14079 |
43531-43549 |
JJ |
denotes |
anti-neurofilament |
T14080 |
43550-43551 |
-LRB- |
denotes |
( |
T14081 |
43573-43583 |
NNP |
denotes |
Collection |
T14082 |
43551-43559 |
NNP |
denotes |
American |
T14083 |
43560-43564 |
NNP |
denotes |
Type |
T14084 |
43565-43572 |
NNP |
denotes |
Culture |
T14085 |
43583-43585 |
, |
denotes |
, |
T14086 |
43585-43593 |
NNP |
denotes |
Manassas |
T14087 |
43593-43595 |
, |
denotes |
, |
T14088 |
43595-43603 |
NNP |
denotes |
Virginia |
T14089 |
43603-43605 |
, |
denotes |
, |
T14090 |
43605-43611 |
NNP |
denotes |
United |
T14091 |
43612-43618 |
NNP |
denotes |
States |
T14092 |
43618-43619 |
-RRB- |
denotes |
) |
T14093 |
43619-43621 |
, |
denotes |
, |
T14094 |
43621-43626 |
NN |
denotes |
sheep |
T14095 |
43627-43636 |
JJ |
denotes |
anti-eGFP |
T14096 |
43637-43638 |
-LRB- |
denotes |
( |
T14097 |
43638-43648 |
NNP |
denotes |
Biogenesis |
T14098 |
43648-43650 |
, |
denotes |
, |
T14099 |
43650-43655 |
NNP |
denotes |
Poole |
T14100 |
43655-43657 |
, |
denotes |
, |
T14101 |
43657-43663 |
NNP |
denotes |
United |
T14102 |
43664-43671 |
NNP |
denotes |
Kingdom |
T14103 |
43671-43672 |
-RRB- |
denotes |
) |
T14104 |
43672-43674 |
, |
denotes |
, |
T14105 |
43674-43678 |
NN |
denotes |
goat |
T14106 |
43679-43688 |
JJ |
denotes |
anti-LacZ |
T14107 |
43689-43690 |
-LRB- |
denotes |
[ |
T14108 |
43690-43692 |
CD |
denotes |
14 |
T14109 |
43692-43693 |
-RRB- |
denotes |
] |
T14110 |
43693-43695 |
, |
denotes |
, |
T14111 |
43695-43699 |
NN |
denotes |
goat |
T14112 |
43700-43709 |
JJ |
denotes |
anti-TrkC |
T14113 |
43710-43711 |
-LRB- |
denotes |
( |
T14114 |
43711-43715 |
NN |
denotes |
gift |
T14115 |
43716-43720 |
IN |
denotes |
from |
T14116 |
43721-43723 |
NNP |
denotes |
L. |
T14117 |
43727-43736 |
NNP |
denotes |
Reichardt |
T14118 |
43724-43726 |
NNP |
denotes |
F. |
T14119 |
43736-43737 |
-RRB- |
denotes |
) |
T14120 |
43737-43739 |
, |
denotes |
, |
T14121 |
43739-43742 |
CC |
denotes |
and |
T14122 |
43743-43749 |
NN |
denotes |
guinea |
T14123 |
43750-43753 |
NN |
denotes |
pig |
T14124 |
43754-43763 |
JJ |
denotes |
anti-Isl1 |
T14125 |
43764-43765 |
-LRB- |
denotes |
[ |
T14126 |
43765-43767 |
CD |
denotes |
14 |
T14127 |
43767-43768 |
-RRB- |
denotes |
] |
T14128 |
43768-43769 |
. |
denotes |
. |
T14129 |
43769-43995 |
sentence |
denotes |
Terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) to detect apoptotic cells in E13.5 DRG on cryostat sections was performed as described by the manufacturer (Roche, Basel, Switzerland). |
T14130 |
43770-43778 |
JJ |
denotes |
Terminal |
T14131 |
43796-43807 |
NN |
denotes |
transferase |
T14132 |
43779-43795 |
NN |
denotes |
deoxynucleotidyl |
T14133 |
43808-43816 |
VBN |
denotes |
mediated |
T14134 |
43807-43808 |
HYPH |
denotes |
- |
T14135 |
43843-43851 |
NN |
denotes |
labeling |
T14136 |
43817-43829 |
VBN |
denotes |
biotinylated |
T14137 |
43830-43833 |
NN |
denotes |
UTP |
T14138 |
43834-43838 |
NN |
denotes |
nick |
T14139 |
43839-43842 |
NN |
denotes |
end |
T14140 |
43924-43933 |
VBN |
denotes |
performed |
T14141 |
43852-43853 |
-LRB- |
denotes |
( |
T14142 |
43853-43858 |
NN |
denotes |
TUNEL |
T14143 |
43858-43859 |
-RRB- |
denotes |
) |
T14144 |
43860-43862 |
TO |
denotes |
to |
T14145 |
43863-43869 |
VB |
denotes |
detect |
T14146 |
43870-43879 |
JJ |
denotes |
apoptotic |
T14147 |
43880-43885 |
NNS |
denotes |
cells |
T14148 |
43886-43888 |
IN |
denotes |
in |
T14149 |
43889-43894 |
NN |
denotes |
E13.5 |
T14150 |
43895-43898 |
NN |
denotes |
DRG |
T14151 |
43899-43901 |
IN |
denotes |
on |
T14152 |
43902-43910 |
NN |
denotes |
cryostat |
T14153 |
43911-43919 |
NNS |
denotes |
sections |
T14154 |
43920-43923 |
VBD |
denotes |
was |
T14155 |
43934-43936 |
IN |
denotes |
as |
T14156 |
43937-43946 |
VBN |
denotes |
described |
T14157 |
43947-43949 |
IN |
denotes |
by |
T14158 |
43950-43953 |
DT |
denotes |
the |
T14159 |
43954-43966 |
NN |
denotes |
manufacturer |
T14160 |
43967-43968 |
-LRB- |
denotes |
( |
T14161 |
43968-43973 |
NNP |
denotes |
Roche |
T14162 |
43973-43975 |
, |
denotes |
, |
T14163 |
43975-43980 |
NNP |
denotes |
Basel |
T14164 |
43980-43982 |
, |
denotes |
, |
T14165 |
43982-43993 |
NNP |
denotes |
Switzerland |
T14166 |
43993-43994 |
-RRB- |
denotes |
) |
T14167 |
43994-43995 |
. |
denotes |
. |
T14168 |
43995-44082 |
sentence |
denotes |
Quantitative analysis of TUNEL+ DRG cells was performed essentially as described [27]. |
T14169 |
43996-44008 |
JJ |
denotes |
Quantitative |
T14170 |
44009-44017 |
NN |
denotes |
analysis |
T14171 |
44042-44051 |
VBN |
denotes |
performed |
T14172 |
44018-44020 |
IN |
denotes |
of |
T14173 |
44021-44026 |
NN |
denotes |
TUNEL |
T14174 |
44032-44037 |
NNS |
denotes |
cells |
T14175 |
44026-44027 |
SYM |
denotes |
+ |
T14176 |
44028-44031 |
NN |
denotes |
DRG |
T14177 |
44038-44041 |
VBD |
denotes |
was |
T14178 |
44052-44063 |
RB |
denotes |
essentially |
T14179 |
44067-44076 |
VBN |
denotes |
described |
T14180 |
44064-44066 |
IN |
denotes |
as |
T14181 |
44077-44078 |
-LRB- |
denotes |
[ |
T14182 |
44078-44080 |
CD |
denotes |
27 |
T14183 |
44080-44081 |
-RRB- |
denotes |
] |
T14184 |
44081-44082 |
. |
denotes |
. |
T14185 |
44082-44186 |
sentence |
denotes |
BrdU pulse-chase experiments and LacZ wholemount stainings were performed as previously described [46]. |
T14186 |
44083-44087 |
NN |
denotes |
BrdU |
T14187 |
44100-44111 |
NNS |
denotes |
experiments |
T14188 |
44088-44093 |
NN |
denotes |
pulse |
T14189 |
44094-44099 |
NN |
denotes |
chase |
T14190 |
44093-44094 |
HYPH |
denotes |
- |
T14191 |
44147-44156 |
VBN |
denotes |
performed |
T14192 |
44112-44115 |
CC |
denotes |
and |
T14193 |
44116-44120 |
NN |
denotes |
LacZ |
T14194 |
44132-44141 |
NNS |
denotes |
stainings |
T14195 |
44121-44131 |
NN |
denotes |
wholemount |
T14196 |
44142-44146 |
VBD |
denotes |
were |
T14197 |
44157-44159 |
IN |
denotes |
as |
T14198 |
44171-44180 |
VBN |
denotes |
described |
T14199 |
44160-44170 |
RB |
denotes |
previously |
T14200 |
44181-44182 |
-LRB- |
denotes |
[ |
T14202 |
44184-44185 |
-RRB- |
denotes |
] |
T14203 |
44185-44186 |
. |
denotes |
. |
T14204 |
44186-44458 |
sentence |
denotes |
For anterograde tracing experiments to visualize projections of sensory neurons, rhodamine-conjugated dextran (Molecular Probes) was injected into single lumbar (L3) DRG at E13.5 or applied to whole lumbar dorsal roots (L3) at postnatal day (P) 5 using glass capillaries. |
T14205 |
44187-44190 |
IN |
denotes |
For |
T14206 |
44226-44235 |
VB |
denotes |
visualize |
T14207 |
44191-44202 |
JJ |
denotes |
anterograde |
T14208 |
44211-44222 |
NNS |
denotes |
experiments |
T14209 |
44203-44210 |
NN |
denotes |
tracing |
T14210 |
44223-44225 |
TO |
denotes |
to |
T14211 |
44320-44328 |
VBN |
denotes |
injected |
T14212 |
44236-44247 |
NNS |
denotes |
projections |
T14213 |
44248-44250 |
IN |
denotes |
of |
T14214 |
44251-44258 |
JJ |
denotes |
sensory |
T14215 |
44259-44266 |
NNS |
denotes |
neurons |
T14216 |
44266-44268 |
, |
denotes |
, |
T14217 |
44268-44277 |
NN |
denotes |
rhodamine |
T14218 |
44278-44288 |
VBN |
denotes |
conjugated |
T14219 |
44277-44278 |
HYPH |
denotes |
- |
T14220 |
44289-44296 |
NN |
denotes |
dextran |
T14221 |
44297-44298 |
-LRB- |
denotes |
( |
T14222 |
44308-44314 |
NNP |
denotes |
Probes |
T14223 |
44298-44307 |
NNP |
denotes |
Molecular |
T14224 |
44314-44315 |
-RRB- |
denotes |
) |
T14225 |
44316-44319 |
VBD |
denotes |
was |
T14226 |
44329-44333 |
IN |
denotes |
into |
T14227 |
44334-44340 |
JJ |
denotes |
single |
T14228 |
44353-44356 |
NN |
denotes |
DRG |
T14229 |
44341-44347 |
NN |
denotes |
lumbar |
T14230 |
44348-44349 |
-LRB- |
denotes |
( |
T14231 |
44349-44351 |
NN |
denotes |
L3 |
T14232 |
44351-44352 |
-RRB- |
denotes |
) |
T14233 |
44357-44359 |
IN |
denotes |
at |
T14234 |
44360-44365 |
NN |
denotes |
E13.5 |
T14235 |
44366-44368 |
CC |
denotes |
or |
T14236 |
44369-44376 |
VBN |
denotes |
applied |
T14237 |
44377-44379 |
IN |
denotes |
to |
T14238 |
44380-44385 |
JJ |
denotes |
whole |
T14239 |
44400-44405 |
NNS |
denotes |
roots |
T14240 |
44386-44392 |
JJ |
denotes |
lumbar |
T14241 |
44393-44399 |
JJ |
denotes |
dorsal |
T14242 |
44406-44407 |
-LRB- |
denotes |
( |
T14243 |
44407-44409 |
NN |
denotes |
L3 |
T14244 |
44409-44410 |
-RRB- |
denotes |
) |
T14245 |
44411-44413 |
IN |
denotes |
at |
T14246 |
44414-44423 |
JJ |
denotes |
postnatal |
T14247 |
44424-44427 |
NN |
denotes |
day |
T14248 |
44428-44429 |
-LRB- |
denotes |
( |
T14249 |
44429-44430 |
NN |
denotes |
P |
T14250 |
44430-44431 |
-RRB- |
denotes |
) |
T14251 |
44432-44433 |
CD |
denotes |
5 |
T14252 |
44434-44439 |
VBG |
denotes |
using |
T14253 |
44440-44445 |
NN |
denotes |
glass |
T14254 |
44446-44457 |
NNS |
denotes |
capillaries |
T14255 |
44457-44458 |
. |
denotes |
. |
T14256 |
44458-44535 |
sentence |
denotes |
After injection, animals were incubated for 2–3 h (E13.5) or overnight (P5). |
T14257 |
44459-44464 |
IN |
denotes |
After |
T14258 |
44489-44498 |
VBN |
denotes |
incubated |
T14259 |
44465-44474 |
NN |
denotes |
injection |
T14260 |
44474-44476 |
, |
denotes |
, |
T14261 |
44476-44483 |
NNS |
denotes |
animals |
T14262 |
44484-44488 |
VBD |
denotes |
were |
T14263 |
44499-44502 |
IN |
denotes |
for |
T14264 |
44503-44504 |
CD |
denotes |
2 |
T14265 |
44505-44506 |
CD |
denotes |
3 |
T14266 |
44504-44505 |
SYM |
denotes |
– |
T14267 |
44507-44508 |
NN |
denotes |
h |
T14268 |
44509-44510 |
-LRB- |
denotes |
( |
T14269 |
44510-44515 |
NN |
denotes |
E13.5 |
T14270 |
44515-44516 |
-RRB- |
denotes |
) |
T14271 |
44517-44519 |
CC |
denotes |
or |
T14272 |
44520-44529 |
RB |
denotes |
overnight |
T14273 |
44530-44531 |
-LRB- |
denotes |
( |
T14274 |
44531-44533 |
NN |
denotes |
P5 |
T14275 |
44533-44534 |
-RRB- |
denotes |
) |
T14276 |
44534-44535 |
. |
denotes |
. |
T14277 |
44535-44690 |
sentence |
denotes |
Cryostat sections were processed for immunohistochemistry as described [14] using fluorophore-conjugated secondary antibodies (1:1,000, Molecular Probes). |
T14278 |
44536-44544 |
NN |
denotes |
Cryostat |
T14279 |
44545-44553 |
NNS |
denotes |
sections |
T14280 |
44559-44568 |
VBN |
denotes |
processed |
T14281 |
44554-44558 |
VBD |
denotes |
were |
T14282 |
44569-44572 |
IN |
denotes |
for |
T14283 |
44573-44593 |
NN |
denotes |
immunohistochemistry |
T14284 |
44594-44596 |
IN |
denotes |
as |
T14285 |
44597-44606 |
VBN |
denotes |
described |
T14286 |
44607-44608 |
-LRB- |
denotes |
[ |
T14287 |
44608-44610 |
CD |
denotes |
14 |
T14288 |
44610-44611 |
-RRB- |
denotes |
] |
T14289 |
44612-44617 |
VBG |
denotes |
using |
T14290 |
44618-44629 |
NN |
denotes |
fluorophore |
T14291 |
44630-44640 |
VBN |
denotes |
conjugated |
T14292 |
44629-44630 |
HYPH |
denotes |
- |
T14293 |
44651-44661 |
NNS |
denotes |
antibodies |
T14294 |
44641-44650 |
JJ |
denotes |
secondary |
T14295 |
44662-44663 |
-LRB- |
denotes |
( |
T14296 |
44682-44688 |
NNP |
denotes |
Probes |
T14297 |
44663-44664 |
CD |
denotes |
1 |
T14298 |
44664-44665 |
SYM |
denotes |
: |
T14299 |
44665-44670 |
CD |
denotes |
1,000 |
T14300 |
44670-44672 |
, |
denotes |
, |
T14301 |
44672-44681 |
NNP |
denotes |
Molecular |
T14302 |
44688-44689 |
-RRB- |
denotes |
) |
T14303 |
44689-44690 |
. |
denotes |
. |
T14304 |
44690-44762 |
sentence |
denotes |
Images were collected on an Olympus (Tokyo, Japan) confocal microscope. |
T14305 |
44691-44697 |
NNS |
denotes |
Images |
T14306 |
44703-44712 |
VBN |
denotes |
collected |
T14307 |
44698-44702 |
VBD |
denotes |
were |
T14308 |
44713-44715 |
IN |
denotes |
on |
T14309 |
44716-44718 |
DT |
denotes |
an |
T14310 |
44751-44761 |
NN |
denotes |
microscope |
T14311 |
44719-44726 |
NNP |
denotes |
Olympus |
T14312 |
44727-44728 |
-LRB- |
denotes |
( |
T14313 |
44728-44733 |
NNP |
denotes |
Tokyo |
T14314 |
44733-44735 |
, |
denotes |
, |
T14315 |
44735-44740 |
NNP |
denotes |
Japan |
T14316 |
44740-44741 |
-RRB- |
denotes |
) |
T14317 |
44742-44750 |
JJ |
denotes |
confocal |
T14318 |
44761-44762 |
. |
denotes |
. |
T14319 |
44762-45026 |
sentence |
denotes |
Images from in situ hybridization experiments were collected with an RT-SPOT camera (Diagnostic Instruments, Sterling Heights, Michigan, United States), and Corel (Eden Prairie, Minnesota, United States) Photo Paint 10.0 was used for digital processing of images. |
T14320 |
44763-44769 |
NNS |
denotes |
Images |
T14321 |
44814-44823 |
VBN |
denotes |
collected |
T14322 |
44770-44774 |
IN |
denotes |
from |
T14323 |
44775-44777 |
FW |
denotes |
in |
T14324 |
44778-44782 |
FW |
denotes |
situ |
T14325 |
44797-44808 |
NNS |
denotes |
experiments |
T14326 |
44783-44796 |
NN |
denotes |
hybridization |
T14327 |
44809-44813 |
VBD |
denotes |
were |
T14328 |
44824-44828 |
IN |
denotes |
with |
T14329 |
44829-44831 |
DT |
denotes |
an |
T14330 |
44840-44846 |
NN |
denotes |
camera |
T14331 |
44832-44834 |
NN |
denotes |
RT |
T14332 |
44835-44839 |
NN |
denotes |
SPOT |
T14333 |
44834-44835 |
HYPH |
denotes |
- |
T14334 |
44847-44848 |
-LRB- |
denotes |
( |
T14335 |
44859-44870 |
NNPS |
denotes |
Instruments |
T14336 |
44848-44858 |
JJ |
denotes |
Diagnostic |
T14337 |
44870-44872 |
, |
denotes |
, |
T14338 |
44872-44880 |
NNP |
denotes |
Sterling |
T14339 |
44881-44888 |
NNP |
denotes |
Heights |
T14340 |
44888-44890 |
, |
denotes |
, |
T14341 |
44890-44898 |
NNP |
denotes |
Michigan |
T14342 |
44898-44900 |
, |
denotes |
, |
T14343 |
44900-44906 |
NNP |
denotes |
United |
T14344 |
44907-44913 |
NNP |
denotes |
States |
T14345 |
44913-44914 |
-RRB- |
denotes |
) |
T14346 |
44914-44916 |
, |
denotes |
, |
T14347 |
44916-44919 |
CC |
denotes |
and |
T14348 |
44920-44925 |
NNP |
denotes |
Corel |
T14349 |
44973-44978 |
NNP |
denotes |
Paint |
T14350 |
44926-44927 |
-LRB- |
denotes |
( |
T14351 |
44932-44939 |
NNP |
denotes |
Prairie |
T14352 |
44927-44931 |
NNP |
denotes |
Eden |
T14353 |
44939-44941 |
, |
denotes |
, |
T14354 |
44941-44950 |
NNP |
denotes |
Minnesota |
T14355 |
44950-44952 |
, |
denotes |
, |
T14356 |
44952-44958 |
NNP |
denotes |
United |
T14357 |
44959-44965 |
NNP |
denotes |
States |
T14358 |
44965-44966 |
-RRB- |
denotes |
) |
T14359 |
44967-44972 |
NNP |
denotes |
Photo |
T14360 |
44988-44992 |
VBN |
denotes |
used |
T14361 |
44979-44983 |
CD |
denotes |
10.0 |
T14362 |
44984-44987 |
VBD |
denotes |
was |
T14363 |
44993-44996 |
IN |
denotes |
for |
T14364 |
44997-45004 |
JJ |
denotes |
digital |
T14365 |
45005-45015 |
NN |
denotes |
processing |
T14366 |
45016-45018 |
IN |
denotes |
of |
T14367 |
45019-45025 |
NNS |
denotes |
images |
T14368 |
45025-45026 |
. |
denotes |
. |
T14469 |
45028-45030 |
FW |
denotes |
In |
T14470 |
45031-45036 |
FW |
denotes |
vitro |
T14471 |
45037-45045 |
NNS |
denotes |
cultures |
T14472 |
45046-45048 |
IN |
denotes |
of |
T14473 |
45049-45052 |
NN |
denotes |
DRG |
T14474 |
45052-45464 |
sentence |
denotes |
Individual lumbar DRG were dissected from E13.5 or E14.5 embryos and placed on Matrigel (BD Biosciences, San Jose, California, United States) coated coverslips in DMEM/F12 (Gibco, San Diego, California, United States), 2 mM L-Gln (Gibco), N2 (Gibco), and 1 mg/ml BSA (Sigma, St. Louis, Missouri, United States) without neurotrophins, or supplemented with either NGF (100 ng/ml, Gibco) or NT-3 (20 ng/ml, Sigma). |
T14475 |
45053-45063 |
JJ |
denotes |
Individual |
T14476 |
45071-45074 |
NN |
denotes |
DRG |
T14477 |
45064-45070 |
NN |
denotes |
lumbar |
T14478 |
45080-45089 |
VBN |
denotes |
dissected |
T14479 |
45075-45079 |
VBD |
denotes |
were |
T14480 |
45090-45094 |
IN |
denotes |
from |
T14481 |
45095-45100 |
NN |
denotes |
E13.5 |
T14482 |
45110-45117 |
NNS |
denotes |
embryos |
T14483 |
45101-45103 |
CC |
denotes |
or |
T14484 |
45104-45109 |
NN |
denotes |
E14.5 |
T14485 |
45118-45121 |
CC |
denotes |
and |
T14486 |
45122-45128 |
VBN |
denotes |
placed |
T14487 |
45129-45131 |
IN |
denotes |
on |
T14488 |
45132-45140 |
NNP |
denotes |
Matrigel |
T14489 |
45195-45201 |
VBN |
denotes |
coated |
T14490 |
45141-45142 |
-LRB- |
denotes |
( |
T14491 |
45145-45156 |
NNP |
denotes |
Biosciences |
T14492 |
45142-45144 |
NNP |
denotes |
BD |
T14493 |
45156-45158 |
, |
denotes |
, |
T14494 |
45158-45161 |
NNP |
denotes |
San |
T14495 |
45162-45166 |
NNP |
denotes |
Jose |
T14496 |
45166-45168 |
, |
denotes |
, |
T14497 |
45168-45178 |
NNP |
denotes |
California |
T14498 |
45178-45180 |
, |
denotes |
, |
T14499 |
45180-45186 |
NNP |
denotes |
United |
T14500 |
45187-45193 |
NNP |
denotes |
States |
T14501 |
45193-45194 |
-RRB- |
denotes |
) |
T14502 |
45202-45212 |
NNS |
denotes |
coverslips |
T14503 |
45213-45215 |
IN |
denotes |
in |
T14504 |
45216-45220 |
NN |
denotes |
DMEM |
T14505 |
45221-45224 |
NN |
denotes |
F12 |
T14506 |
45220-45221 |
HYPH |
denotes |
/ |
T14507 |
45225-45226 |
-LRB- |
denotes |
( |
T14508 |
45226-45231 |
NNP |
denotes |
Gibco |
T14509 |
45231-45233 |
, |
denotes |
, |
T14510 |
45233-45236 |
NNP |
denotes |
San |
T14511 |
45237-45242 |
NNP |
denotes |
Diego |
T14512 |
45242-45244 |
, |
denotes |
, |
T14513 |
45244-45254 |
NNP |
denotes |
California |
T14514 |
45254-45256 |
, |
denotes |
, |
T14515 |
45256-45262 |
NNP |
denotes |
United |
T14516 |
45263-45269 |
NNP |
denotes |
States |
T14517 |
45269-45270 |
-RRB- |
denotes |
) |
T14518 |
45270-45272 |
, |
denotes |
, |
T14519 |
45272-45273 |
CD |
denotes |
2 |
T14520 |
45274-45276 |
NN |
denotes |
mM |
T14521 |
45279-45282 |
NN |
denotes |
Gln |
T14522 |
45277-45278 |
NN |
denotes |
L |
T14523 |
45278-45279 |
HYPH |
denotes |
- |
T14524 |
45283-45284 |
-LRB- |
denotes |
( |
T14525 |
45284-45289 |
NNP |
denotes |
Gibco |
T14526 |
45289-45290 |
-RRB- |
denotes |
) |
T14527 |
45290-45292 |
, |
denotes |
, |
T14528 |
45292-45294 |
NN |
denotes |
N2 |
T14529 |
45295-45296 |
-LRB- |
denotes |
( |
T14530 |
45296-45301 |
NNP |
denotes |
Gibco |
T14531 |
45301-45302 |
-RRB- |
denotes |
) |
T14532 |
45302-45304 |
, |
denotes |
, |
T14533 |
45304-45307 |
CC |
denotes |
and |
T14534 |
45308-45309 |
CD |
denotes |
1 |
T14535 |
45310-45312 |
NN |
denotes |
mg |
T14536 |
45316-45319 |
NN |
denotes |
BSA |
T14537 |
45312-45313 |
SYM |
denotes |
/ |
T14538 |
45313-45315 |
NN |
denotes |
ml |
T14539 |
45320-45321 |
-LRB- |
denotes |
( |
T14540 |
45321-45326 |
NNP |
denotes |
Sigma |
T14541 |
45326-45328 |
, |
denotes |
, |
T14542 |
45328-45331 |
NNP |
denotes |
St. |
T14543 |
45332-45337 |
NNP |
denotes |
Louis |
T14544 |
45337-45339 |
, |
denotes |
, |
T14545 |
45339-45347 |
NNP |
denotes |
Missouri |
T14546 |
45347-45349 |
, |
denotes |
, |
T14547 |
45349-45355 |
NNP |
denotes |
United |
T14548 |
45356-45362 |
NNP |
denotes |
States |
T14549 |
45362-45363 |
-RRB- |
denotes |
) |
T14550 |
45364-45371 |
IN |
denotes |
without |
T14551 |
45372-45385 |
NNS |
denotes |
neurotrophins |
T14552 |
45385-45387 |
, |
denotes |
, |
T14553 |
45387-45389 |
CC |
denotes |
or |
T14554 |
45390-45402 |
VBN |
denotes |
supplemented |
T14555 |
45403-45407 |
IN |
denotes |
with |
T14556 |
45408-45414 |
CC |
denotes |
either |
T14557 |
45415-45418 |
NN |
denotes |
NGF |
T14558 |
45419-45420 |
-LRB- |
denotes |
( |
T14559 |
45431-45436 |
NNP |
denotes |
Gibco |
T14560 |
45420-45423 |
CD |
denotes |
100 |
T14561 |
45424-45426 |
NN |
denotes |
ng |
T14562 |
45426-45427 |
SYM |
denotes |
/ |
T14563 |
45427-45429 |
NN |
denotes |
ml |
T14564 |
45429-45431 |
, |
denotes |
, |
T14565 |
45436-45437 |
-RRB- |
denotes |
) |
T14566 |
45438-45440 |
CC |
denotes |
or |
T14567 |
45441-45443 |
NN |
denotes |
NT |
T14568 |
45443-45444 |
HYPH |
denotes |
- |
T14569 |
45444-45445 |
CD |
denotes |
3 |
T14570 |
45446-45447 |
-LRB- |
denotes |
( |
T14571 |
45457-45462 |
NNP |
denotes |
Sigma |
T14572 |
45447-45449 |
CD |
denotes |
20 |
T14573 |
45450-45452 |
NN |
denotes |
ng |
T14574 |
45452-45453 |
SYM |
denotes |
/ |
T14575 |
45453-45455 |
NN |
denotes |
ml |
T14576 |
45455-45457 |
, |
denotes |
, |
T14577 |
45462-45463 |
-RRB- |
denotes |
) |
T14578 |
45463-45464 |
. |
denotes |
. |
T14579 |
45464-45604 |
sentence |
denotes |
DRG explants (n ≥ 20 for each condition) were cultured for 48 h, processed for immunocytochemistry, and analyzed using confocal microscopy. |
T14580 |
45465-45468 |
NN |
denotes |
DRG |
T14581 |
45469-45477 |
NNS |
denotes |
explants |
T14582 |
45511-45519 |
VBN |
denotes |
cultured |
T14583 |
45478-45479 |
-LRB- |
denotes |
( |
T14584 |
45483-45485 |
CD |
denotes |
20 |
T14585 |
45479-45480 |
NN |
denotes |
n |
T14586 |
45481-45482 |
SYM |
denotes |
≥ |
T14587 |
45486-45489 |
IN |
denotes |
for |
T14588 |
45490-45494 |
DT |
denotes |
each |
T14589 |
45495-45504 |
NN |
denotes |
condition |
T14590 |
45504-45505 |
-RRB- |
denotes |
) |
T14591 |
45506-45510 |
VBD |
denotes |
were |
T14592 |
45520-45523 |
IN |
denotes |
for |
T14593 |
45524-45526 |
CD |
denotes |
48 |
T14594 |
45527-45528 |
NN |
denotes |
h |
T14595 |
45528-45530 |
, |
denotes |
, |
T14596 |
45530-45539 |
VBN |
denotes |
processed |
T14597 |
45540-45543 |
IN |
denotes |
for |
T14598 |
45544-45563 |
NN |
denotes |
immunocytochemistry |
T14599 |
45563-45565 |
, |
denotes |
, |
T14600 |
45565-45568 |
CC |
denotes |
and |
T14601 |
45569-45577 |
VBN |
denotes |
analyzed |
T14602 |
45578-45583 |
VBG |
denotes |
using |
T14603 |
45584-45592 |
JJ |
denotes |
confocal |
T14604 |
45593-45603 |
NN |
denotes |
microscopy |
T14605 |
45603-45604 |
. |
denotes |
. |
T14754 |
45606-45613 |
NNP |
denotes |
Western |
T14755 |
45614-45618 |
NN |
denotes |
blot |
T14756 |
45619-45627 |
NN |
denotes |
analysis |
T14757 |
45627-45843 |
sentence |
denotes |
Lumbar DRG from E16.5 embryos were isolated, mechanically disrupted, homogenized using glass beads (Sigma), and lysed in standard lysis buffer supplemented with protease and phosphatase inhibitors as described [47]. |
T14758 |
45628-45634 |
NN |
denotes |
Lumbar |
T14759 |
45635-45638 |
NN |
denotes |
DRG |
T14760 |
45663-45671 |
VBN |
denotes |
isolated |
T14761 |
45639-45643 |
IN |
denotes |
from |
T14762 |
45644-45649 |
NN |
denotes |
E16.5 |
T14763 |
45650-45657 |
NNS |
denotes |
embryos |
T14764 |
45658-45662 |
VBD |
denotes |
were |
T14765 |
45671-45673 |
, |
denotes |
, |
T14766 |
45673-45685 |
RB |
denotes |
mechanically |
T14767 |
45686-45695 |
VBN |
denotes |
disrupted |
T14768 |
45695-45697 |
, |
denotes |
, |
T14769 |
45697-45708 |
VBN |
denotes |
homogenized |
T14770 |
45709-45714 |
VBG |
denotes |
using |
T14771 |
45715-45720 |
NN |
denotes |
glass |
T14772 |
45721-45726 |
NNS |
denotes |
beads |
T14773 |
45727-45728 |
-LRB- |
denotes |
( |
T14774 |
45728-45733 |
NNP |
denotes |
Sigma |
T14775 |
45733-45734 |
-RRB- |
denotes |
) |
T14776 |
45734-45736 |
, |
denotes |
, |
T14777 |
45736-45739 |
CC |
denotes |
and |
T14778 |
45740-45745 |
VBN |
denotes |
lysed |
T14779 |
45746-45748 |
IN |
denotes |
in |
T14780 |
45749-45757 |
JJ |
denotes |
standard |
T14781 |
45764-45770 |
NN |
denotes |
buffer |
T14782 |
45758-45763 |
NN |
denotes |
lysis |
T14783 |
45771-45783 |
VBN |
denotes |
supplemented |
T14784 |
45784-45788 |
IN |
denotes |
with |
T14785 |
45789-45797 |
NN |
denotes |
protease |
T14786 |
45814-45824 |
NNS |
denotes |
inhibitors |
T14787 |
45798-45801 |
CC |
denotes |
and |
T14788 |
45802-45813 |
NN |
denotes |
phosphatase |
T14789 |
45825-45827 |
IN |
denotes |
as |
T14790 |
45828-45837 |
VBN |
denotes |
described |
T14791 |
45838-45839 |
-LRB- |
denotes |
[ |
T14792 |
45839-45841 |
CD |
denotes |
47 |
T14793 |
45841-45842 |
-RRB- |
denotes |
] |
T14794 |
45842-45843 |
. |
denotes |
. |
T14795 |
45843-46133 |
sentence |
denotes |
Protein extracts were resolved by SDS-PAGE, and immunoblotting was performed using antibodies against Akt, p-Akt (Ser473), CREB, p-CREB (Ser133), Bax, Bcl-xl (Cell Signaling Technology, Beverly, Massachusetts, United States), and Bcl2 (BD Pharmingen, San Diego, California, United States). |
T14796 |
45844-45851 |
NN |
denotes |
Protein |
T14797 |
45852-45860 |
NNS |
denotes |
extracts |
T14798 |
45866-45874 |
VBN |
denotes |
resolved |
T14799 |
45861-45865 |
VBD |
denotes |
were |
T14800 |
45875-45877 |
IN |
denotes |
by |
T14801 |
45878-45881 |
NN |
denotes |
SDS |
T14802 |
45882-45886 |
NN |
denotes |
PAGE |
T14803 |
45881-45882 |
HYPH |
denotes |
- |
T14804 |
45886-45888 |
, |
denotes |
, |
T14805 |
45888-45891 |
CC |
denotes |
and |
T14806 |
45892-45906 |
NN |
denotes |
immunoblotting |
T14807 |
45911-45920 |
VBN |
denotes |
performed |
T14808 |
45907-45910 |
VBD |
denotes |
was |
T14809 |
45921-45926 |
VBG |
denotes |
using |
T14810 |
45927-45937 |
NNS |
denotes |
antibodies |
T14811 |
45938-45945 |
IN |
denotes |
against |
T14812 |
45946-45949 |
NN |
denotes |
Akt |
T14813 |
45949-45951 |
, |
denotes |
, |
T14814 |
45951-45952 |
NN |
denotes |
p |
T14815 |
45953-45956 |
NN |
denotes |
Akt |
T14816 |
45952-45953 |
HYPH |
denotes |
- |
T14817 |
45957-45958 |
-LRB- |
denotes |
( |
T14818 |
45958-45964 |
NN |
denotes |
Ser473 |
T14819 |
45964-45965 |
-RRB- |
denotes |
) |
T14820 |
45965-45967 |
, |
denotes |
, |
T14821 |
45967-45971 |
NN |
denotes |
CREB |
T14822 |
45971-45973 |
, |
denotes |
, |
T14823 |
45973-45974 |
NN |
denotes |
p |
T14824 |
45975-45979 |
NN |
denotes |
CREB |
T14825 |
45974-45975 |
HYPH |
denotes |
- |
T14826 |
45980-45981 |
-LRB- |
denotes |
( |
T14827 |
45981-45987 |
NN |
denotes |
Ser133 |
T14828 |
45987-45988 |
-RRB- |
denotes |
) |
T14829 |
45988-45990 |
, |
denotes |
, |
T14830 |
45990-45993 |
NN |
denotes |
Bax |
T14831 |
45993-45995 |
, |
denotes |
, |
T14832 |
45995-45998 |
NN |
denotes |
Bcl |
T14833 |
45999-46001 |
NN |
denotes |
xl |
T14834 |
45998-45999 |
HYPH |
denotes |
- |
T14835 |
46002-46003 |
-LRB- |
denotes |
( |
T14836 |
46018-46028 |
NNP |
denotes |
Technology |
T14837 |
46003-46007 |
NNP |
denotes |
Cell |
T14838 |
46008-46017 |
NNP |
denotes |
Signaling |
T14839 |
46028-46030 |
, |
denotes |
, |
T14840 |
46030-46037 |
NNP |
denotes |
Beverly |
T14841 |
46037-46039 |
, |
denotes |
, |
T14842 |
46039-46052 |
NNP |
denotes |
Massachusetts |
T14843 |
46052-46054 |
, |
denotes |
, |
T14844 |
46054-46060 |
NNP |
denotes |
United |
T14845 |
46061-46067 |
NNP |
denotes |
States |
T14846 |
46067-46068 |
-RRB- |
denotes |
) |
T14847 |
46068-46070 |
, |
denotes |
, |
T14848 |
46070-46073 |
CC |
denotes |
and |
T14849 |
46074-46078 |
NN |
denotes |
Bcl2 |
T14850 |
46079-46080 |
-LRB- |
denotes |
( |
T14851 |
46083-46093 |
NNP |
denotes |
Pharmingen |
T14852 |
46080-46082 |
NN |
denotes |
BD |
T14853 |
46093-46095 |
, |
denotes |
, |
T14854 |
46095-46098 |
NNP |
denotes |
San |
T14855 |
46099-46104 |
NNP |
denotes |
Diego |
T14856 |
46104-46106 |
, |
denotes |
, |
T14857 |
46106-46116 |
NNP |
denotes |
California |
T14858 |
46116-46118 |
, |
denotes |
, |
T14859 |
46118-46124 |
NNP |
denotes |
United |
T14860 |
46125-46131 |
NNP |
denotes |
States |
T14861 |
46131-46132 |
-RRB- |
denotes |
) |
T14862 |
46132-46133 |
. |
denotes |
. |
T14863 |
46133-46336 |
sentence |
denotes |
For quantification, films (X-OMAT AR, Eastman Kodak, Rochester, New York, United States) were scanned and densitometry was performed using IMAGEQUANT 5.2 (Molecular Dynamics, Amersham, Uppsala, Sweden). |
T14864 |
46134-46137 |
IN |
denotes |
For |
T14865 |
46228-46235 |
VBN |
denotes |
scanned |
T14866 |
46138-46152 |
NN |
denotes |
quantification |
T14867 |
46152-46154 |
, |
denotes |
, |
T14868 |
46154-46159 |
NNS |
denotes |
films |
T14869 |
46160-46161 |
-LRB- |
denotes |
( |
T14870 |
46168-46170 |
NNP |
denotes |
AR |
T14871 |
46161-46162 |
NN |
denotes |
X |
T14872 |
46163-46167 |
NN |
denotes |
OMAT |
T14873 |
46162-46163 |
HYPH |
denotes |
- |
T14874 |
46170-46172 |
, |
denotes |
, |
T14875 |
46172-46179 |
NNP |
denotes |
Eastman |
T14876 |
46180-46185 |
NNP |
denotes |
Kodak |
T14877 |
46185-46187 |
, |
denotes |
, |
T14878 |
46187-46196 |
NNP |
denotes |
Rochester |
T14879 |
46196-46198 |
, |
denotes |
, |
T14880 |
46198-46201 |
NNP |
denotes |
New |
T14881 |
46202-46206 |
NNP |
denotes |
York |
T14882 |
46206-46208 |
, |
denotes |
, |
T14883 |
46208-46214 |
NNP |
denotes |
United |
T14884 |
46215-46221 |
NNP |
denotes |
States |
T14885 |
46221-46222 |
-RRB- |
denotes |
) |
T14886 |
46223-46227 |
VBD |
denotes |
were |
T14887 |
46236-46239 |
CC |
denotes |
and |
T14888 |
46240-46252 |
NN |
denotes |
densitometry |
T14889 |
46257-46266 |
VBN |
denotes |
performed |
T14890 |
46253-46256 |
VBD |
denotes |
was |
T14891 |
46267-46272 |
VBG |
denotes |
using |
T14892 |
46273-46283 |
NNP |
denotes |
IMAGEQUANT |
T14893 |
46284-46287 |
CD |
denotes |
5.2 |
T14894 |
46288-46289 |
-LRB- |
denotes |
( |
T14895 |
46299-46307 |
NNP |
denotes |
Dynamics |
T14896 |
46289-46298 |
NNP |
denotes |
Molecular |
T14897 |
46307-46309 |
, |
denotes |
, |
T14898 |
46309-46317 |
NNP |
denotes |
Amersham |
T14899 |
46317-46319 |
, |
denotes |
, |
T14900 |
46319-46326 |
NNP |
denotes |
Uppsala |
T14901 |
46326-46328 |
, |
denotes |
, |
T14902 |
46328-46334 |
NNP |
denotes |
Sweden |
T14903 |
46334-46335 |
-RRB- |
denotes |
) |
T14904 |
46335-46336 |
. |
denotes |
. |
T15020 |
46338-46355 |
NN |
denotes |
Electrophysiology |
T15021 |
46355-46429 |
sentence |
denotes |
Electrophysiological analysis was performed as previously described [48]. |
T15022 |
46356-46376 |
JJ |
denotes |
Electrophysiological |
T15023 |
46377-46385 |
NN |
denotes |
analysis |
T15024 |
46390-46399 |
VBN |
denotes |
performed |
T15025 |
46386-46389 |
VBD |
denotes |
was |
T15026 |
46400-46402 |
IN |
denotes |
as |
T15027 |
46414-46423 |
VBN |
denotes |
described |
T15028 |
46403-46413 |
RB |
denotes |
previously |
T15029 |
46424-46425 |
-LRB- |
denotes |
[ |
T15030 |
46425-46427 |
CD |
denotes |
48 |
T15031 |
46427-46428 |
-RRB- |
denotes |
] |
T15032 |
46428-46429 |
. |
denotes |
. |
T15033 |
46429-46558 |
sentence |
denotes |
Briefly, intracellular recordings from identified quadriceps motor neurons were made using sharp electrodes (75–120 MΩ, 3M KCl). |
T15034 |
46430-46437 |
RB |
denotes |
Briefly |
T15035 |
46510-46514 |
VBN |
denotes |
made |
T15036 |
46437-46439 |
, |
denotes |
, |
T15037 |
46439-46452 |
JJ |
denotes |
intracellular |
T15038 |
46453-46463 |
NNS |
denotes |
recordings |
T15039 |
46464-46468 |
IN |
denotes |
from |
T15040 |
46469-46479 |
VBN |
denotes |
identified |
T15041 |
46497-46504 |
NNS |
denotes |
neurons |
T15042 |
46480-46490 |
NNS |
denotes |
quadriceps |
T15043 |
46491-46496 |
NN |
denotes |
motor |
T15044 |
46505-46509 |
VBD |
denotes |
were |
T15045 |
46515-46520 |
VBG |
denotes |
using |
T15046 |
46521-46526 |
JJ |
denotes |
sharp |
T15047 |
46527-46537 |
NNS |
denotes |
electrodes |
T15048 |
46538-46539 |
-LRB- |
denotes |
( |
T15049 |
46546-46548 |
NN |
denotes |
MΩ |
T15050 |
46539-46541 |
CD |
denotes |
75 |
T15051 |
46542-46545 |
CD |
denotes |
120 |
T15052 |
46541-46542 |
SYM |
denotes |
– |
T15053 |
46548-46550 |
, |
denotes |
, |
T15054 |
46550-46552 |
NN |
denotes |
3M |
T15055 |
46553-46556 |
NN |
denotes |
KCl |
T15056 |
46556-46557 |
-RRB- |
denotes |
) |
T15057 |
46557-46558 |
. |
denotes |
. |
T15058 |
46558-46758 |
sentence |
denotes |
Average responses (10–20 trials) from suprathreshold nerve stimulation (1.5 times the strength that evokes maximal monosynaptic response) of the quadriceps nerve were acquired with LTP software [49]. |
T15059 |
46559-46566 |
JJ |
denotes |
Average |
T15060 |
46567-46576 |
NNS |
denotes |
responses |
T15061 |
46726-46734 |
VBN |
denotes |
acquired |
T15062 |
46577-46578 |
-LRB- |
denotes |
( |
T15063 |
46584-46590 |
NNS |
denotes |
trials |
T15064 |
46578-46580 |
CD |
denotes |
10 |
T15065 |
46581-46583 |
CD |
denotes |
20 |
T15066 |
46580-46581 |
SYM |
denotes |
– |
T15067 |
46590-46591 |
-RRB- |
denotes |
) |
T15068 |
46592-46596 |
IN |
denotes |
from |
T15069 |
46597-46611 |
NN |
denotes |
suprathreshold |
T15070 |
46618-46629 |
NN |
denotes |
stimulation |
T15071 |
46612-46617 |
NN |
denotes |
nerve |
T15072 |
46630-46631 |
-LRB- |
denotes |
( |
T15073 |
46645-46653 |
NN |
denotes |
strength |
T15074 |
46631-46634 |
CD |
denotes |
1.5 |
T15075 |
46635-46640 |
NNS |
denotes |
times |
T15076 |
46641-46644 |
DT |
denotes |
the |
T15077 |
46654-46658 |
WDT |
denotes |
that |
T15078 |
46659-46665 |
VBZ |
denotes |
evokes |
T15079 |
46666-46673 |
JJ |
denotes |
maximal |
T15080 |
46687-46695 |
NN |
denotes |
response |
T15081 |
46674-46686 |
JJ |
denotes |
monosynaptic |
T15082 |
46695-46696 |
-RRB- |
denotes |
) |
T15083 |
46697-46699 |
IN |
denotes |
of |
T15084 |
46700-46703 |
DT |
denotes |
the |
T15085 |
46715-46720 |
NN |
denotes |
nerve |
T15086 |
46704-46714 |
NNS |
denotes |
quadriceps |
T15087 |
46721-46725 |
VBD |
denotes |
were |
T15088 |
46735-46739 |
IN |
denotes |
with |
T15089 |
46740-46743 |
NN |
denotes |
LTP |
T15090 |
46744-46752 |
NN |
denotes |
software |
T15091 |
46753-46754 |
-LRB- |
denotes |
[ |
T15092 |
46754-46756 |
CD |
denotes |
49 |
T15093 |
46756-46757 |
-RRB- |
denotes |
] |
T15094 |
46757-46758 |
. |
denotes |
. |
T15095 |
46758-46856 |
sentence |
denotes |
Only cells with stable resting potentials more negative than −50 mV were considered for analysis. |
T15096 |
46759-46763 |
RB |
denotes |
Only |
T15097 |
46764-46769 |
NNS |
denotes |
cells |
T15098 |
46832-46842 |
VBN |
denotes |
considered |
T15099 |
46770-46774 |
IN |
denotes |
with |
T15100 |
46775-46781 |
JJ |
denotes |
stable |
T15101 |
46790-46800 |
NNS |
denotes |
potentials |
T15102 |
46782-46789 |
NN |
denotes |
resting |
T15103 |
46801-46805 |
RBR |
denotes |
more |
T15104 |
46806-46814 |
JJ |
denotes |
negative |
T15105 |
46815-46819 |
IN |
denotes |
than |
T15106 |
46820-46821 |
SYM |
denotes |
− |
T15107 |
46821-46823 |
CD |
denotes |
50 |
T15108 |
46824-46826 |
NN |
denotes |
mV |
T15109 |
46827-46831 |
VBD |
denotes |
were |
T15110 |
46843-46846 |
IN |
denotes |
for |
T15111 |
46847-46855 |
NN |
denotes |
analysis |
T15112 |
46855-46856 |
. |
denotes |
. |
T15113 |
46856-47036 |
sentence |
denotes |
Monosynaptic amplitudes were determined offline using custom routines in the Matlab environment (The Mathworks, Natick, Massachusetts, United States) as previously described [48]. |
T15114 |
46857-46869 |
JJ |
denotes |
Monosynaptic |
T15115 |
46870-46880 |
NNS |
denotes |
amplitudes |
T15116 |
46886-46896 |
VBN |
denotes |
determined |
T15117 |
46881-46885 |
VBD |
denotes |
were |
T15118 |
46897-46904 |
RB |
denotes |
offline |
T15119 |
46905-46910 |
VBG |
denotes |
using |
T15120 |
46911-46917 |
NN |
denotes |
custom |
T15121 |
46918-46926 |
NNS |
denotes |
routines |
T15122 |
46927-46929 |
IN |
denotes |
in |
T15123 |
46930-46933 |
DT |
denotes |
the |
T15124 |
46941-46952 |
NN |
denotes |
environment |
T15125 |
46934-46940 |
NNP |
denotes |
Matlab |
T15126 |
46953-46954 |
-LRB- |
denotes |
( |
T15127 |
46958-46967 |
NNP |
denotes |
Mathworks |
T15128 |
46954-46957 |
DT |
denotes |
The |
T15129 |
46967-46969 |
, |
denotes |
, |
T15130 |
46969-46975 |
NNP |
denotes |
Natick |
T15131 |
46975-46977 |
, |
denotes |
, |
T15132 |
46977-46990 |
NNP |
denotes |
Massachusetts |
T15133 |
46990-46992 |
, |
denotes |
, |
T15134 |
46992-46998 |
NNP |
denotes |
United |
T15135 |
46999-47005 |
NNP |
denotes |
States |
T15136 |
47005-47006 |
-RRB- |
denotes |
) |
T15137 |
47007-47009 |
IN |
denotes |
as |
T15138 |
47021-47030 |
VBN |
denotes |
described |
T15139 |
47010-47020 |
RB |
denotes |
previously |
T15140 |
47031-47032 |
-LRB- |
denotes |
[ |
T15141 |
47032-47034 |
CD |
denotes |
48 |
T15142 |
47034-47035 |
-RRB- |
denotes |
] |
T15143 |
47035-47036 |
. |
denotes |
. |
T260 |
0-1 |
DT |
denotes |
A |
T14201 |
44182-44184 |
CD |
denotes |
46 |
T7751 |
26046-26053 |
NN |
denotes |
support |
T11732 |
38618-38622 |
RB |
denotes |
also |
T11733 |
38623-38627 |
VBN |
denotes |
been |
T11734 |
38634-38636 |
TO |
denotes |
to |
T11735 |
38637-38644 |
VB |
denotes |
exhibit |
T11736 |
38645-38653 |
JJ |
denotes |
distinct |
T11737 |
38654-38663 |
NNS |
denotes |
functions |
T11738 |
38664-38666 |
IN |
denotes |
at |
T11739 |
38667-38678 |
JJ |
denotes |
progressive |
T11740 |
38679-38684 |
NNS |
denotes |
steps |
T11741 |
38685-38687 |
IN |
denotes |
of |
T11742 |
38688-38695 |
NN |
denotes |
lineage |
T11743 |
38696-38709 |
NN |
denotes |
specification |
T11744 |
38710-38711 |
-LRB- |
denotes |
[ |
T11745 |
38711-38713 |
CD |
denotes |
40 |
T11746 |
38713-38714 |
-RRB- |
denotes |
] |
T11747 |
38714-38715 |
. |
denotes |
. |
T11748 |
38715-39083 |
sentence |
denotes |
Analysis of the mechanisms by which transcription factor programs can be shifted over time to control different complements of downstream genes and thus different aspects of neuronal and cellular fates in progenitor cells or post-mitotic neurons may provide further insight into the way in which transcription factors act to control the assembly of neuronal circuits. |
T11749 |
38716-38724 |
NN |
denotes |
Analysis |
T11750 |
38966-38973 |
VB |
denotes |
provide |
T11751 |
38725-38727 |
IN |
denotes |
of |
T11752 |
38728-38731 |
DT |
denotes |
the |
T11753 |
38732-38742 |
NNS |
denotes |
mechanisms |
T11754 |
38743-38745 |
IN |
denotes |
by |
T11755 |
38789-38796 |
VBN |
denotes |
shifted |
T11756 |
38746-38751 |
WDT |
denotes |
which |
T11757 |
38752-38765 |
NN |
denotes |
transcription |
T11758 |
38766-38772 |
NN |
denotes |
factor |
T11759 |
38773-38781 |
NNS |
denotes |
programs |
T11760 |
38782-38785 |
MD |
denotes |
can |
T11761 |
38786-38788 |
VB |
denotes |
be |
T11762 |
38797-38801 |
IN |
denotes |
over |
T11763 |
38802-38806 |
NN |
denotes |
time |
R91 |
T356 |
T357 |
amod |
precocious,expression |
R7 |
T263 |
T261 |
amod |
Developmental,Switch |
R8 |
T264 |
T261 |
prep |
in,Switch |
R9 |
T265 |
T266 |
det |
the,Response |
R10 |
T266 |
T264 |
pobj |
Response,in |
R11 |
T267 |
T266 |
prep |
of,Response |
R12 |
T268 |
T269 |
compound |
DRG,Neurons |
R13 |
T269 |
T267 |
pobj |
Neurons,of |
R14 |
T270 |
T266 |
prep |
to,Response |
R15 |
T271 |
T272 |
compound |
ETS,Signaling |
R16 |
T272 |
T270 |
pobj |
Signaling,to |
R17 |
T273 |
T274 |
compound |
Transcription,Factor |
R18 |
T274 |
T272 |
compound |
Factor,Signaling |
R19 |
T277 |
T278 |
nummod |
Two,factors |
R20 |
T278 |
T281 |
nsubjpass |
factors,induced |
R21 |
T279 |
T278 |
compound |
ETS,factors |
R22 |
T280 |
T278 |
compound |
transcription,factors |
R23 |
T282 |
T278 |
prep |
of,factors |
R24 |
T283 |
T284 |
det |
the,subfamily |
R25 |
T284 |
T282 |
pobj |
subfamily,of |
R26 |
T285 |
T284 |
compound |
Pea3,subfamily |
R27 |
T286 |
T281 |
auxpass |
are,induced |
R28 |
T287 |
T281 |
prep |
in,induced |
R29 |
T288 |
T287 |
pobj |
subpopulations,in |
R30 |
T289 |
T288 |
prep |
of,subpopulations |
R31 |
T290 |
T291 |
amod |
dorsal,ganglion |
R32 |
T291 |
T293 |
nmod |
ganglion,neurons |
R33 |
T292 |
T291 |
nmod |
root,ganglion |
R34 |
T293 |
T289 |
pobj |
neurons,of |
R35 |
T294 |
T291 |
punct |
(,ganglion |
R36 |
T295 |
T291 |
appos |
DRG,ganglion |
R37 |
T296 |
T291 |
punct |
),ganglion |
R38 |
T297 |
T293 |
amod |
sensory,neurons |
R39 |
T298 |
T297 |
cc |
and,sensory |
R40 |
T299 |
T300 |
amod |
spinal,motor |
R41 |
T300 |
T297 |
conj |
motor,sensory |
R42 |
T301 |
T281 |
agent |
by,induced |
R43 |
T302 |
T303 |
npadvmod |
target,derived |
R44 |
T303 |
T305 |
amod |
derived,factors |
R45 |
T304 |
T303 |
punct |
-,derived |
R46 |
T305 |
T301 |
pobj |
factors,by |
R47 |
T306 |
T281 |
punct |
.,induced |
R48 |
T308 |
T309 |
poss |
Their,expression |
R49 |
T309 |
T310 |
nsubj |
expression,controls |
R50 |
T311 |
T312 |
amod |
late,aspects |
R51 |
T312 |
T310 |
dobj |
aspects,controls |
R52 |
T313 |
T312 |
prep |
of,aspects |
R53 |
T314 |
T315 |
amod |
neuronal,differentiation |
R54 |
T315 |
T313 |
pobj |
differentiation,of |
R55 |
T316 |
T317 |
amod |
such,as |
R56 |
T317 |
T312 |
prep |
as,aspects |
R57 |
T318 |
T319 |
compound |
target,invasion |
R58 |
T319 |
T317 |
pobj |
invasion,as |
R59 |
T320 |
T319 |
cc |
and,invasion |
R60 |
T321 |
T319 |
conj |
branching,invasion |
R61 |
T322 |
T310 |
punct |
.,controls |
R62 |
T324 |
T325 |
advmod |
Here,show |
R63 |
T326 |
T325 |
punct |
", ",show |
R64 |
T327 |
T325 |
nsubj |
we,show |
R65 |
T328 |
T329 |
mark |
that,is |
R66 |
T329 |
T325 |
ccomp |
is,show |
R67 |
T330 |
T331 |
det |
the,onset |
R68 |
T331 |
T329 |
nsubj |
onset,is |
R69 |
T332 |
T331 |
amod |
late,onset |
R70 |
T333 |
T331 |
prep |
of,onset |
R71 |
T334 |
T335 |
compound |
ETS,gene |
R72 |
T335 |
T336 |
compound |
gene,expression |
R73 |
T336 |
T333 |
pobj |
expression,of |
R74 |
T337 |
T338 |
det |
an,requirement |
R75 |
T338 |
T329 |
attr |
requirement,is |
R76 |
T339 |
T338 |
amod |
essential,requirement |
R77 |
T340 |
T338 |
prep |
for,requirement |
R78 |
T341 |
T342 |
amod |
normal,differentiation |
R79 |
T342 |
T340 |
pobj |
differentiation,for |
R80 |
T343 |
T344 |
amod |
sensory,neuron |
R81 |
T344 |
T342 |
compound |
neuron,differentiation |
R82 |
T345 |
T325 |
punct |
.,show |
R83 |
T347 |
T348 |
nsubj |
We,provide |
R84 |
T349 |
T350 |
amod |
genetic,evidence |
R85 |
T350 |
T348 |
dobj |
evidence,provide |
R86 |
T351 |
T348 |
prep |
in,provide |
R87 |
T352 |
T353 |
det |
the,mouse |
R88 |
T353 |
T351 |
pobj |
mouse,in |
R89 |
T354 |
T355 |
mark |
that,perturbs |
R90 |
T355 |
T348 |
ccomp |
perturbs,provide |
R92 |
T357 |
T355 |
nsubj |
expression,perturbs |
R93 |
T358 |
T357 |
compound |
ETS,expression |
R94 |
T359 |
T357 |
prep |
in,expression |
R95 |
T360 |
T361 |
nmod |
DRG,neurons |
R96 |
T361 |
T359 |
pobj |
neurons,in |
R97 |
T362 |
T361 |
amod |
sensory,neurons |
R98 |
T363 |
T364 |
amod |
axonal,projections |
R99 |
T364 |
T355 |
dobj |
projections,perturbs |
R100 |
T365 |
T364 |
punct |
", ",projections |
R101 |
T366 |
T367 |
det |
the,acquisition |
R102 |
T367 |
T364 |
conj |
acquisition,projections |
R103 |
T368 |
T367 |
prep |
of,acquisition |
R104 |
T369 |
T370 |
amod |
terminal,markers |
R105 |
T370 |
T368 |
pobj |
markers,of |
R106 |
T371 |
T370 |
compound |
differentiation,markers |
R107 |
T372 |
T367 |
punct |
", ",acquisition |
R108 |
T373 |
T367 |
cc |
and,acquisition |
R109 |
T374 |
T375 |
poss |
their,dependence |
R110 |
T375 |
T367 |
conj |
dependence,acquisition |
R111 |
T376 |
T375 |
prep |
on,dependence |
R112 |
T377 |
T378 |
amod |
neurotrophic,support |
R113 |
T378 |
T376 |
pobj |
support,on |
R114 |
T379 |
T348 |
punct |
.,provide |
R115 |
T381 |
T382 |
advmod |
Together,indicate |
R116 |
T383 |
T382 |
punct |
", ",indicate |
R117 |
T384 |
T385 |
poss |
our,findings |
R118 |
T385 |
T382 |
nsubj |
findings,indicate |
R119 |
T386 |
T387 |
mark |
that,exhibit |
R120 |
T387 |
T382 |
ccomp |
exhibit,indicate |
R121 |
T388 |
T389 |
nmod |
DRG,neurons |
R122 |
T389 |
T387 |
nsubj |
neurons,exhibit |
R123 |
T390 |
T389 |
amod |
sensory,neurons |
R124 |
T391 |
T392 |
det |
a,switch |
R125 |
T392 |
T387 |
dobj |
switch,exhibit |
R126 |
T393 |
T392 |
amod |
temporal,switch |
R127 |
T394 |
T392 |
amod |
developmental,switch |
R128 |
T395 |
T396 |
dep |
that,revealed |
R129 |
T396 |
T392 |
relcl |
revealed,switch |
R130 |
T397 |
T396 |
aux |
can,revealed |
R131 |
T398 |
T396 |
auxpass |
be,revealed |
R132 |
T399 |
T396 |
agent |
by,revealed |
R133 |
T400 |
T401 |
amod |
distinct,responses |
R134 |
T401 |
T399 |
pobj |
responses,by |
R135 |
T402 |
T401 |
prep |
to,responses |
R136 |
T403 |
T404 |
compound |
ETS,signaling |
R137 |
T404 |
T402 |
pobj |
signaling,to |
R138 |
T405 |
T406 |
compound |
transcription,factor |
R139 |
T406 |
T404 |
compound |
factor,signaling |
R140 |
T407 |
T401 |
prep |
at,responses |
R141 |
T408 |
T409 |
amod |
sequential,steps |
R142 |
T409 |
T407 |
pobj |
steps,at |
R143 |
T410 |
T409 |
prep |
of,steps |
R144 |
T411 |
T412 |
amod |
neuronal,maturation |
R145 |
T412 |
T410 |
pobj |
maturation,of |
R146 |
T413 |
T382 |
punct |
.,indicate |
R147 |
T260 |
T261 |
det |
A,Switch |
R156 |
T1510 |
T1511 |
amod |
Neuronal,differentiation |
R157 |
T1511 |
T1512 |
nsubj |
differentiation,is |
R158 |
T1513 |
T1514 |
det |
a,process |
R159 |
T1514 |
T1512 |
attr |
process,is |
R160 |
T1515 |
T1514 |
amod |
protracted,process |
R161 |
T1516 |
T1517 |
prep |
during,express |
R162 |
T1517 |
T1514 |
relcl |
express,process |
R163 |
T1518 |
T1516 |
pobj |
which,during |
R164 |
T1519 |
T1520 |
advmod |
newly,generated |
R165 |
T1520 |
T1521 |
amod |
generated,neurons |
R166 |
T1521 |
T1517 |
nsubj |
neurons,express |
R167 |
T1522 |
T1523 |
amod |
distinct,programs |
R168 |
T1523 |
T1517 |
dative |
programs,express |
R169 |
T1524 |
T1523 |
amod |
cellular,programs |
R170 |
T1525 |
T1524 |
cc |
and,cellular |
R171 |
T1526 |
T1524 |
conj |
molecular,cellular |
R172 |
T1527 |
T1517 |
prep |
at,express |
R173 |
T1528 |
T1529 |
amod |
precise,times |
R174 |
T1529 |
T1527 |
pobj |
times,at |
R175 |
T1530 |
T1517 |
prep |
during,express |
R176 |
T1531 |
T1532 |
poss |
their,maturation |
R177 |
T1532 |
T1530 |
pobj |
maturation,during |
R178 |
T1533 |
T1517 |
punct |
: ,express |
R179 |
T1534 |
T1535 |
amod |
long,distance |
R180 |
T1535 |
T1537 |
compound |
distance,outgrowth |
R181 |
T1536 |
T1535 |
punct |
-,distance |
R182 |
T1537 |
T1517 |
dobj |
outgrowth,express |
R183 |
T1538 |
T1537 |
compound |
axon,outgrowth |
R184 |
T1539 |
T1537 |
punct |
", ",outgrowth |
R185 |
T1540 |
T1541 |
amod |
subsequent,branching |
R186 |
T1541 |
T1537 |
conj |
branching,outgrowth |
R187 |
T1542 |
T1541 |
amod |
terminal,branching |
R188 |
T1543 |
T1541 |
punct |
", ",branching |
R189 |
T1544 |
T1541 |
cc |
and,branching |
R190 |
T1545 |
T1546 |
advmod |
finally,synaptogenesis |
R191 |
T1546 |
T1541 |
conj |
synaptogenesis,branching |
R192 |
T1547 |
T1512 |
punct |
.,is |
R193 |
T1549 |
T1550 |
amod |
Many,aspects |
R194 |
T1550 |
T1552 |
nsubj |
aspects,appear |
R195 |
T1551 |
T1550 |
amod |
important,aspects |
R196 |
T1553 |
T1550 |
prep |
of,aspects |
R197 |
T1554 |
T1555 |
amod |
neuronal,character |
R198 |
T1555 |
T1553 |
pobj |
character,of |
R199 |
T1556 |
T1557 |
aux |
to,acquired |
R200 |
T1557 |
T1552 |
xcomp |
acquired,appear |
R201 |
T1558 |
T1557 |
auxpass |
be,acquired |
R202 |
T1559 |
T1557 |
prep |
through,acquired |
R203 |
T1560 |
T1561 |
det |
the,expression |
R204 |
T1561 |
T1559 |
pobj |
expression,through |
R205 |
T1562 |
T1561 |
prep |
of,expression |
R206 |
T1563 |
T1564 |
compound |
transcription,factors |
R207 |
T1564 |
T1562 |
pobj |
factors,of |
R208 |
T1565 |
T1561 |
prep |
at,expression |
R209 |
T1566 |
T1567 |
compound |
progenitor,stages |
R210 |
T1567 |
T1565 |
pobj |
stages,at |
R211 |
T1568 |
T1567 |
compound |
cell,stages |
R212 |
T1569 |
T1552 |
punct |
", ",appear |
R213 |
T1570 |
T1571 |
mark |
whereas,depend |
R214 |
T1571 |
T1552 |
advcl |
depend,appear |
R215 |
T1572 |
T1571 |
nsubj |
others,depend |
R216 |
T1573 |
T1571 |
prep |
on,depend |
R217 |
T1574 |
T1573 |
pobj |
expression,on |
R218 |
T1575 |
T1576 |
advmod |
immediately,upon |
R219 |
T1576 |
T1571 |
prep |
upon,depend |
R220 |
T1577 |
T1578 |
compound |
cell,cycle |
R221 |
T1578 |
T1579 |
compound |
cycle,exit |
R222 |
T1579 |
T1576 |
pobj |
exit,upon |
R223 |
T1580 |
T1581 |
punct |
[,1 |
R224 |
T1581 |
T1571 |
parataxis |
1,depend |
R225 |
T1582 |
T1581 |
punct |
],1 |
R226 |
T1583 |
T1552 |
punct |
.,appear |
R227 |
T1585 |
T1586 |
cc |
But,has |
R228 |
T1587 |
T1588 |
mark |
whether,is |
R229 |
T1588 |
T1586 |
csubj |
is,has |
R230 |
T1589 |
T1590 |
det |
the,expression |
R231 |
T1590 |
T1588 |
nsubj |
expression,is |
R232 |
T1591 |
T1590 |
amod |
orderly,expression |
R233 |
T1592 |
T1590 |
cc |
and,expression |
R234 |
T1593 |
T1590 |
conj |
activity,expression |
R235 |
T1594 |
T1590 |
prep |
of,expression |
R236 |
T1595 |
T1596 |
amod |
transcriptional,programs |
R237 |
T1596 |
T1594 |
pobj |
programs,of |
R238 |
T1597 |
T1590 |
prep |
at,expression |
R239 |
T1598 |
T1599 |
advmod |
much,later |
R240 |
T1599 |
T1600 |
amod |
later,stages |
R241 |
T1600 |
T1597 |
pobj |
stages,at |
R242 |
T1601 |
T1600 |
amod |
developmental,stages |
R243 |
T1602 |
T1590 |
punct |
", ",expression |
R244 |
T1603 |
T1604 |
advmod |
well,after |
R245 |
T1604 |
T1590 |
prep |
after,expression |
R246 |
T1605 |
T1606 |
compound |
cell,cycle |
R247 |
T1606 |
T1607 |
compound |
cycle,exit |
R248 |
T1607 |
T1604 |
pobj |
exit,after |
R249 |
T1608 |
T1588 |
punct |
", ",is |
R250 |
T1609 |
T1610 |
det |
an,step |
R251 |
T1610 |
T1588 |
attr |
step,is |
R252 |
T1611 |
T1610 |
amod |
essential,step |
R253 |
T1612 |
T1610 |
prep |
in,step |
R254 |
T1613 |
T1614 |
det |
the,progression |
R255 |
T1614 |
T1612 |
pobj |
progression,in |
R256 |
T1615 |
T1614 |
prep |
of,progression |
R257 |
T1616 |
T1617 |
amod |
neuronal,differentiation |
R258 |
T1617 |
T1615 |
pobj |
differentiation,of |
R259 |
T1618 |
T1617 |
cc |
and,differentiation |
R260 |
T1619 |
T1620 |
compound |
circuit,assembly |
R261 |
T1620 |
T1617 |
conj |
assembly,differentiation |
R262 |
T1621 |
T1586 |
advmod |
yet,has |
R263 |
T1622 |
T1623 |
aux |
to,resolved |
R264 |
T1623 |
T1586 |
xcomp |
resolved,has |
R265 |
T1624 |
T1623 |
auxpass |
be,resolved |
R266 |
T1625 |
T1586 |
punct |
.,has |
R267 |
T1627 |
T1628 |
det |
The,differentiation |
R268 |
T1628 |
T1629 |
nsubjpass |
differentiation,studied |
R269 |
T1630 |
T1628 |
prep |
of,differentiation |
R270 |
T1631 |
T1632 |
amod |
sensory,neurons |
R271 |
T1632 |
T1630 |
pobj |
neurons,of |
R272 |
T1633 |
T1628 |
prep |
of,differentiation |
R273 |
T1634 |
T1635 |
amod |
dorsal,ganglia |
R274 |
T1635 |
T1633 |
pobj |
ganglia,of |
R275 |
T1636 |
T1635 |
compound |
root,ganglia |
R276 |
T1637 |
T1635 |
punct |
(,ganglia |
R277 |
T1638 |
T1635 |
appos |
DRG,ganglia |
R278 |
T1639 |
T1629 |
punct |
),studied |
R279 |
T1640 |
T1629 |
aux |
has,studied |
R280 |
T1641 |
T1629 |
auxpass |
been,studied |
R281 |
T1642 |
T1629 |
advmod |
extensively,studied |
R282 |
T1643 |
T1629 |
prep |
with,studied |
R283 |
T1644 |
T1643 |
pobj |
respect,with |
R284 |
T1645 |
T1644 |
prep |
to,respect |
R285 |
T1646 |
T1647 |
amod |
inductive,events |
R286 |
T1647 |
T1645 |
pobj |
events,to |
R287 |
T1648 |
T1649 |
dep |
that,specify |
R288 |
T1649 |
T1647 |
relcl |
specify,events |
R289 |
T1650 |
T1651 |
amod |
neuronal,fate |
R290 |
T1651 |
T1649 |
dobj |
fate,specify |
R291 |
T1652 |
T1653 |
punct |
[,3 |
R292 |
T1653 |
T1651 |
parataxis |
3,fate |
R293 |
T1654 |
T1653 |
nummod |
2,3 |
R294 |
T1655 |
T1653 |
punct |
",",3 |
R295 |
T1656 |
T1653 |
punct |
],3 |
R296 |
T1657 |
T1651 |
punct |
", ",fate |
R297 |
T1658 |
T1659 |
advmod |
as,as |
R298 |
T1659 |
T1651 |
cc |
as,fate |
R299 |
T1660 |
T1659 |
advmod |
well,as |
R300 |
T1661 |
T1662 |
det |
the,involvement |
R301 |
T1662 |
T1651 |
conj |
involvement,fate |
R302 |
T1663 |
T1662 |
prep |
of,involvement |
R303 |
T1664 |
T1665 |
amod |
late,factors |
R304 |
T1665 |
T1663 |
pobj |
factors,of |
R305 |
T1666 |
T1667 |
npadvmod |
target,derived |
R306 |
T1667 |
T1665 |
amod |
derived,factors |
R307 |
T1668 |
T1667 |
punct |
-,derived |
R308 |
T1669 |
T1665 |
amod |
neurotrophic,factors |
R309 |
T1670 |
T1662 |
prep |
in,involvement |
R310 |
T1671 |
T1672 |
det |
the,control |
R311 |
T1672 |
T1670 |
pobj |
control,in |
R312 |
T1673 |
T1672 |
prep |
of,control |
R313 |
T1674 |
T1675 |
amod |
neuronal,survival |
R314 |
T1675 |
T1673 |
pobj |
survival,of |
R315 |
T1676 |
T1677 |
punct |
[,4 |
R316 |
T1677 |
T1662 |
parataxis |
4,involvement |
R317 |
T1678 |
T1677 |
punct |
],4 |
R318 |
T1679 |
T1629 |
punct |
.,studied |
R319 |
T1681 |
T1682 |
amod |
Recent,evidence |
R320 |
T1682 |
T1683 |
nsubj |
evidence,begun |
R321 |
T1684 |
T1683 |
aux |
has,begun |
R322 |
T1685 |
T1686 |
aux |
to,emerge |
R323 |
T1686 |
T1683 |
xcomp |
emerge,begun |
R324 |
T1687 |
T1688 |
mark |
that,involved |
R325 |
T1688 |
T1683 |
ccomp |
involved,begun |
R326 |
T1689 |
T1690 |
npadvmod |
target,derived |
R327 |
T1690 |
T1692 |
amod |
derived,factors |
R328 |
T1691 |
T1690 |
punct |
-,derived |
R329 |
T1692 |
T1688 |
nsubjpass |
factors,involved |
R330 |
T1693 |
T1688 |
auxpass |
are,involved |
R331 |
T1694 |
T1688 |
advmod |
also,involved |
R332 |
T1695 |
T1688 |
prep |
in,involved |
R333 |
T1696 |
T1695 |
pcomp |
regulating,in |
R334 |
T1697 |
T1698 |
amod |
later,aspects |
R335 |
T1698 |
T1696 |
dobj |
aspects,regulating |
R336 |
T1699 |
T1698 |
prep |
of,aspects |
R337 |
T1700 |
T1701 |
amod |
neuronal,differentiation |
R338 |
T1701 |
T1699 |
pobj |
differentiation,of |
R339 |
T1702 |
T1703 |
punct |
[,7 |
R340 |
T1703 |
T1683 |
parataxis |
7,begun |
R341 |
T1704 |
T1703 |
nummod |
5,7 |
R342 |
T1705 |
T1703 |
punct |
",",7 |
R343 |
T1706 |
T1703 |
nummod |
6,7 |
R344 |
T1707 |
T1703 |
punct |
",",7 |
R345 |
T1708 |
T1703 |
punct |
],7 |
R346 |
T1709 |
T1683 |
punct |
.,begun |
R347 |
T1711 |
T1712 |
prep |
In,addressed |
R348 |
T1713 |
T1711 |
amod |
particular,In |
R349 |
T1714 |
T1712 |
punct |
", ",addressed |
R350 |
T1715 |
T1716 |
amod |
genetic,experiments |
R351 |
T1716 |
T1712 |
nsubj |
experiments,addressed |
R352 |
T1717 |
T1712 |
aux |
have,addressed |
R353 |
T1718 |
T1719 |
det |
the,role |
R354 |
T1719 |
T1712 |
dobj |
role,addressed |
R355 |
T1720 |
T1721 |
npadvmod |
survival,independent |
R356 |
T1721 |
T1719 |
amod |
independent,role |
R357 |
T1722 |
T1721 |
punct |
-,independent |
R358 |
T1723 |
T1719 |
prep |
of,role |
R359 |
T1724 |
T1725 |
amod |
neurotrophic,factors |
R360 |
T1725 |
T1723 |
pobj |
factors,of |
R361 |
T1726 |
T1719 |
prep |
during,role |
R362 |
T1727 |
T1726 |
pobj |
development,during |
R363 |
T1728 |
T1712 |
prep |
by,addressed |
R364 |
T1729 |
T1728 |
pcomp |
exploiting,by |
R365 |
T1730 |
T1729 |
dobj |
strains,exploiting |
R366 |
T1731 |
T1730 |
prep |
of,strains |
R367 |
T1732 |
T1731 |
pobj |
mice,of |
R368 |
T1733 |
T1730 |
amod |
defective,strains |
R369 |
T1734 |
T1735 |
preconj |
both,in |
R370 |
T1735 |
T1733 |
prep |
in,defective |
R371 |
T1736 |
T1737 |
compound |
neurotrophin,signaling |
R372 |
T1737 |
T1735 |
pobj |
signaling,in |
R373 |
T1738 |
T1735 |
cc |
and,in |
R374 |
T1739 |
T1735 |
conj |
in,in |
R375 |
T1740 |
T1741 |
det |
the,function |
R376 |
T1741 |
T1739 |
pobj |
function,in |
R377 |
T1742 |
T1741 |
prep |
of,function |
R378 |
T1743 |
T1744 |
det |
the,Bax |
R379 |
T1744 |
T1742 |
pobj |
Bax,of |
R380 |
T1745 |
T1744 |
amod |
proapoptotic,Bax |
R381 |
T1746 |
T1744 |
compound |
gene,Bax |
R382 |
T1747 |
T1748 |
punct |
[,9 |
R383 |
T1748 |
T1729 |
parataxis |
9,exploiting |
R384 |
T1749 |
T1748 |
nummod |
8,9 |
R385 |
T1750 |
T1748 |
punct |
",",9 |
R386 |
T1751 |
T1748 |
punct |
],9 |
R387 |
T1752 |
T1712 |
punct |
.,addressed |
R388 |
T1754 |
T1755 |
det |
These,studies |
R389 |
T1755 |
T1756 |
nsubj |
studies,revealed |
R390 |
T1863 |
T1864 |
amod |
peptidergic,neurons |
R391 |
T1864 |
T1862 |
pobj |
neurons,of |
R392 |
T1865 |
T1861 |
acl |
expressing,subpopulation |
R393 |
T1866 |
T1865 |
dobj |
Apterous,expressing |
R394 |
T1867 |
T1866 |
cc |
and,Apterous |
R395 |
T1757 |
T1756 |
punct |
", ",revealed |
R396 |
T1868 |
T1866 |
conj |
Squeeze,Apterous |
R397 |
T1758 |
T1756 |
prep |
for,revealed |
R398 |
T1869 |
T1870 |
punct |
[,12 |
R399 |
T1759 |
T1758 |
pobj |
example,for |
R400 |
T1760 |
T1756 |
punct |
", ",revealed |
R401 |
T1870 |
T1846 |
parataxis |
12,control |
R402 |
T1761 |
T1756 |
aux |
have,revealed |
R403 |
T1762 |
T1763 |
mark |
that,controls |
R404 |
T1871 |
T1870 |
nummod |
11,12 |
R405 |
T1763 |
T1756 |
ccomp |
controls,revealed |
R406 |
T1764 |
T1765 |
compound |
neurotrophin,signaling |
R407 |
T1872 |
T1870 |
punct |
",",12 |
R408 |
T1765 |
T1763 |
nsubj |
signaling,controls |
R409 |
T1766 |
T1767 |
det |
the,acquisition |
R410 |
T1767 |
T1763 |
dobj |
acquisition,controls |
R411 |
T1768 |
T1767 |
prep |
of,acquisition |
R412 |
T1769 |
T1770 |
amod |
peptidergic,traits |
R413 |
T1770 |
T1768 |
pobj |
traits,of |
R414 |
T1873 |
T1870 |
punct |
],12 |
R415 |
T1771 |
T1767 |
prep |
in,acquisition |
R416 |
T1772 |
T1773 |
amod |
nociceptive,neurons |
R417 |
T1773 |
T1771 |
pobj |
neurons,in |
R418 |
T1774 |
T1773 |
compound |
DRG,neurons |
R419 |
T1874 |
T1846 |
punct |
.,control |
R420 |
T1775 |
T1767 |
cc |
and,acquisition |
R421 |
T1776 |
T1777 |
det |
the,control |
R422 |
T1876 |
T1877 |
prep |
In,shown |
R423 |
T1777 |
T1767 |
conj |
control,acquisition |
R424 |
T1778 |
T1777 |
prep |
of,control |
R425 |
T1779 |
T1780 |
compound |
target,innervation |
R426 |
T1780 |
T1778 |
pobj |
innervation,of |
R427 |
T1878 |
T1876 |
pobj |
vertebrates,In |
R428 |
T1781 |
T1782 |
punct |
[,9 |
R429 |
T1782 |
T1763 |
parataxis |
9,controls |
R430 |
T1783 |
T1782 |
nummod |
8,9 |
R431 |
T1879 |
T1877 |
punct |
", ",shown |
R432 |
T1784 |
T1782 |
punct |
",",9 |
R433 |
T1785 |
T1782 |
punct |
],9 |
R434 |
T1786 |
T1756 |
punct |
.,revealed |
R435 |
T1880 |
T1881 |
amod |
peripheral,signals |
R436 |
T1881 |
T1877 |
nsubjpass |
signals,shown |
R437 |
T1882 |
T1881 |
amod |
neurotrophic,signals |
R438 |
T1883 |
T1877 |
aux |
have,shown |
R439 |
T1788 |
T1789 |
det |
The,onset |
R440 |
T1789 |
T1790 |
nsubjpass |
onset,shown |
R441 |
T1884 |
T1877 |
auxpass |
been,shown |
R442 |
T1791 |
T1789 |
prep |
of,onset |
R443 |
T1792 |
T1793 |
det |
some,programs |
R444 |
T1885 |
T1886 |
aux |
to,direct |
R445 |
T1793 |
T1791 |
pobj |
programs,of |
R446 |
T1794 |
T1793 |
amod |
transcriptional,programs |
R447 |
T1795 |
T1789 |
prep |
in,onset |
R448 |
T1886 |
T1877 |
xcomp |
direct,shown |
R449 |
T1796 |
T1795 |
pobj |
neurons,in |
R450 |
T1797 |
T1790 |
punct |
", ",shown |
R451 |
T1887 |
T1888 |
det |
the,onset |
R452 |
T1798 |
T1790 |
advmod |
however,shown |
R453 |
T1799 |
T1790 |
punct |
", ",shown |
R454 |
T1888 |
T1886 |
dobj |
onset,direct |
R455 |
T1800 |
T1790 |
aux |
has,shown |
R456 |
T1801 |
T1790 |
advmod |
also,shown |
R457 |
T1889 |
T1888 |
prep |
of,onset |
R458 |
T1802 |
T1790 |
auxpass |
been,shown |
R459 |
T1803 |
T1804 |
aux |
to,occur |
R460 |
T1804 |
T1790 |
xcomp |
occur,shown |
R461 |
T1805 |
T1806 |
advmod |
long,exit |
R462 |
T1806 |
T1804 |
advcl |
exit,occur |
R463 |
T1890 |
T1889 |
pobj |
expression,of |
R464 |
T1807 |
T1806 |
mark |
after,exit |
R465 |
T1808 |
T1806 |
nsubj |
neurons,exit |
R466 |
T1809 |
T1810 |
det |
the,cycle |
R467 |
T1810 |
T1806 |
dobj |
cycle,exit |
R468 |
T1891 |
T1890 |
prep |
of,expression |
R469 |
T1811 |
T1810 |
compound |
cell,cycle |
R470 |
T1812 |
T1790 |
punct |
.,shown |
R471 |
T1892 |
T1893 |
det |
the,factors |
R472 |
T1814 |
T1815 |
det |
An,principle |
R473 |
T1893 |
T1891 |
pobj |
factors,of |
R474 |
T1815 |
T1817 |
nsubj |
principle,is |
R475 |
T1816 |
T1815 |
amod |
emerging,principle |
R476 |
T1894 |
T1893 |
compound |
ETS,factors |
R477 |
T1818 |
T1815 |
prep |
from,principle |
R478 |
T1819 |
T1818 |
pobj |
work,from |
R479 |
T1820 |
T1819 |
prep |
in,work |
R480 |
T1895 |
T1893 |
compound |
transcription,factors |
R481 |
T1821 |
T1820 |
pobj |
Drosophila,in |
R482 |
T1822 |
T1821 |
cc |
and,Drosophila |
R483 |
T1823 |
T1821 |
conj |
vertebrates,Drosophila |
R484 |
T1896 |
T1893 |
appos |
Er81,factors |
R485 |
T1824 |
T1825 |
mark |
that,play |
R486 |
T1825 |
T1817 |
ccomp |
play,is |
R487 |
T1826 |
T1827 |
npadvmod |
target,derived |
R488 |
T1897 |
T1896 |
cc |
and,Er81 |
R489 |
T1827 |
T1829 |
amod |
derived,factors |
R490 |
T1828 |
T1827 |
punct |
-,derived |
R491 |
T1829 |
T1825 |
nsubj |
factors,play |
R492 |
T1898 |
T1896 |
conj |
Pea3,Er81 |
R493 |
T1830 |
T1831 |
det |
a,role |
R494 |
T1831 |
T1825 |
dobj |
role,play |
R495 |
T1899 |
T1886 |
prep |
in,direct |
R496 |
T1832 |
T1831 |
amod |
crucial,role |
R497 |
T1833 |
T1825 |
prep |
in,play |
R498 |
T1900 |
T1901 |
nmod |
DRG,neurons |
R499 |
T1834 |
T1835 |
det |
the,induction |
R500 |
T1835 |
T1833 |
pobj |
induction,in |
R501 |
T1836 |
T1835 |
prep |
of,induction |
R502 |
T1901 |
T1899 |
pobj |
neurons,in |
R503 |
T1837 |
T1838 |
det |
these,programs |
R504 |
T1838 |
T1836 |
pobj |
programs,of |
R505 |
T1902 |
T1901 |
amod |
sensory,neurons |
R506 |
T1839 |
T1838 |
amod |
transcriptional,programs |
R507 |
T1840 |
T1841 |
punct |
[,10 |
R508 |
T1841 |
T1825 |
parataxis |
10,play |
R509 |
T1903 |
T1901 |
cc |
and,neurons |
R510 |
T1842 |
T1841 |
punct |
],10 |
R511 |
T1843 |
T1817 |
punct |
.,is |
R512 |
T1845 |
T1846 |
prep |
In,control |
R513 |
T1904 |
T1905 |
compound |
motor,pools |
R514 |
T1847 |
T1845 |
pobj |
Drosophila,In |
R515 |
T1848 |
T1846 |
punct |
", ",control |
R516 |
T1849 |
T1850 |
amod |
retrograde,signals |
R518 |
T1850 |
T1846 |
nsubj |
signals,control |
R519 |
T1851 |
T1850 |
compound |
BMP,signals |
R520 |
T1852 |
T1850 |
prep |
from,signals |
R521 |
T1906 |
T1905 |
compound |
neuron,pools |
R522 |
T1853 |
T1854 |
det |
the,region |
R523 |
T1907 |
T1908 |
amod |
several,days |
R524 |
T1854 |
T1852 |
pobj |
region,from |
R525 |
T1855 |
T1854 |
compound |
target,region |
R526 |
T1908 |
T1909 |
npadvmod |
days,become |
R527 |
T1856 |
T1857 |
det |
the,differentiation |
R528 |
T1857 |
T1846 |
dobj |
differentiation,control |
R529 |
T1858 |
T1857 |
amod |
terminal,differentiation |
R530 |
T1909 |
T1886 |
advcl |
become,direct |
R531 |
T1859 |
T1857 |
prep |
of,differentiation |
R532 |
T1860 |
T1861 |
det |
a,subpopulation |
R533 |
T1861 |
T1859 |
pobj |
subpopulation,of |
R534 |
T1910 |
T1909 |
mark |
after,become |
R535 |
T1862 |
T1861 |
prep |
of,subpopulation |
R536 |
T1911 |
T1912 |
det |
these,neurons |
R537 |
T1912 |
T1909 |
nsubj |
neurons,become |
R538 |
T1913 |
T1909 |
aux |
have,become |
R539 |
T1914 |
T1909 |
acomp |
post-mitotic,become |
R540 |
T1915 |
T1916 |
punct |
[,16 |
R541 |
T1969 |
T1961 |
punct |
", ",control |
R542 |
T1970 |
T1961 |
prep |
with,control |
R543 |
T1916 |
T1877 |
parataxis |
16,shown |
R544 |
T1971 |
T1972 |
nsubj |
Er81,directing |
R545 |
T1972 |
T1970 |
pobj |
directing,with |
R546 |
T1917 |
T1916 |
nummod |
9,16 |
R547 |
T1973 |
T1974 |
amod |
proprioceptive,neuron |
R548 |
T1974 |
T1976 |
compound |
neuron,differentiation |
R549 |
T1975 |
T1974 |
amod |
sensory,neuron |
R550 |
T1918 |
T1916 |
punct |
",",16 |
R551 |
T1976 |
T1972 |
dobj |
differentiation,directing |
R552 |
T1977 |
T1972 |
cc |
and,directing |
R553 |
T1919 |
T1916 |
nummod |
13,16 |
R554 |
T1978 |
T1979 |
nsubj |
Pea3,directing |
R555 |
T1979 |
T1972 |
conj |
directing,directing |
R556 |
T1920 |
T1916 |
punct |
",",16 |
R557 |
T1980 |
T1981 |
compound |
motor,pool |
R558 |
T1981 |
T1983 |
compound |
pool,differentiation |
R559 |
T1982 |
T1981 |
compound |
neuron,pool |
R560 |
T1921 |
T1916 |
nummod |
14,16 |
R561 |
T1983 |
T1979 |
dobj |
differentiation,directing |
R562 |
T1984 |
T1972 |
punct |
", ",directing |
R563 |
T1922 |
T1916 |
punct |
",",16 |
R564 |
T1985 |
T1972 |
advmod |
respectively,directing |
R565 |
T1986 |
T1987 |
punct |
[,15 |
R566 |
T1987 |
T1961 |
parataxis |
15,control |
R567 |
T1988 |
T1987 |
nummod |
14,15 |
R568 |
T1989 |
T1987 |
punct |
",",15 |
R569 |
T1990 |
T1987 |
punct |
],15 |
R570 |
T1923 |
T1916 |
nummod |
15,16 |
R571 |
T1991 |
T1961 |
punct |
.,control |
R572 |
T1993 |
T1994 |
prep |
In,fail |
R573 |
T1924 |
T1916 |
punct |
",",16 |
R574 |
T1995 |
T1993 |
amod |
particular,In |
R575 |
T1996 |
T1994 |
punct |
", ",fail |
R576 |
T1925 |
T1916 |
punct |
],16 |
R577 |
T1997 |
T1994 |
prep |
in,fail |
R578 |
T1998 |
T1999 |
det |
the,absence |
R579 |
T1926 |
T1877 |
punct |
.,shown |
R580 |
T1999 |
T1997 |
pobj |
absence,in |
R581 |
T2000 |
T1999 |
prep |
of,absence |
R582 |
T2001 |
T2000 |
pobj |
Er81,of |
R583 |
T1928 |
T1929 |
advmod |
Moreover,known |
R584 |
T2002 |
T1999 |
punct |
", ",absence |
R585 |
T2003 |
T1999 |
acl |
achieved,absence |
R586 |
T2004 |
T2003 |
prep |
by,achieved |
R587 |
T2005 |
T2004 |
pobj |
mutation,by |
R588 |
T1930 |
T1929 |
punct |
", ",known |
R589 |
T2006 |
T2005 |
prep |
in,mutation |
R590 |
T2007 |
T2008 |
det |
the,gene |
R591 |
T2008 |
T2006 |
pobj |
gene,in |
R592 |
T2009 |
T2004 |
cc |
or,by |
R593 |
T1931 |
T1932 |
det |
the,induction |
R594 |
T2010 |
T2004 |
conj |
by,by |
R595 |
T2011 |
T2010 |
pobj |
deprivation,by |
R596 |
T1932 |
T1929 |
nsubjpass |
induction,known |
R597 |
T2012 |
T2011 |
prep |
of,deprivation |
R598 |
T2013 |
T2014 |
amod |
peripheral,signaling |
R599 |
T1933 |
T1932 |
prep |
of,induction |
R600 |
T2014 |
T2012 |
pobj |
signaling,of |
R601 |
T2015 |
T2014 |
compound |
neurotrophin,signaling |
R602 |
T1934 |
T1935 |
compound |
Er81,expression |
R603 |
T2016 |
T1994 |
punct |
", ",fail |
R604 |
T2017 |
T2018 |
nmod |
group,Ia |
R605 |
T2018 |
T2019 |
nmod |
Ia,afferents |
R606 |
T1935 |
T1933 |
pobj |
expression,of |
R607 |
T2019 |
T1994 |
nsubj |
afferents,fail |
R608 |
T2020 |
T2019 |
amod |
proprioceptive,afferents |
R609 |
T1936 |
T1932 |
prep |
in,induction |
R610 |
T2021 |
T2022 |
aux |
to,invade |
R611 |
T2022 |
T1994 |
xcomp |
invade,fail |
R612 |
T2023 |
T2024 |
det |
the,cord |
R613 |
T1937 |
T1938 |
amod |
proprioceptive,afferents |
R614 |
T2024 |
T2022 |
dobj |
cord,invade |
R615 |
T2025 |
T2024 |
amod |
ventral,cord |
R616 |
T1938 |
T1936 |
pobj |
afferents,in |
R617 |
T2026 |
T2024 |
amod |
spinal,cord |
R618 |
T2027 |
T2022 |
cc |
and,invade |
R619 |
T2028 |
T2029 |
aux |
to,make |
R620 |
T2029 |
T2022 |
conj |
make,invade |
R621 |
T1939 |
T1929 |
auxpass |
is,known |
R622 |
T1940 |
T1941 |
aux |
to,mediated |
R623 |
T2030 |
T2031 |
amod |
effective,connections |
R624 |
T1941 |
T1929 |
xcomp |
mediated,known |
R625 |
T2031 |
T2029 |
dobj |
connections,make |
R626 |
T2032 |
T2031 |
amod |
synaptic,connections |
R627 |
T2033 |
T2031 |
prep |
with,connections |
R628 |
T1942 |
T1941 |
auxpass |
be,mediated |
R629 |
T2034 |
T2035 |
compound |
motor,neurons |
R630 |
T2035 |
T2033 |
pobj |
neurons,with |
R631 |
T2036 |
T2037 |
punct |
[,14 |
R632 |
T1943 |
T1941 |
agent |
by,mediated |
R633 |
T2037 |
T1994 |
parataxis |
14,fail |
R634 |
T2038 |
T2037 |
nummod |
9,14 |
R635 |
T1944 |
T1945 |
amod |
peripheral,neurotrophin |
R636 |
T2039 |
T2037 |
punct |
",",14 |
R637 |
T2040 |
T2037 |
punct |
],14 |
R638 |
T1945 |
T1943 |
pobj |
neurotrophin,by |
R639 |
T2041 |
T1994 |
punct |
.,fail |
R640 |
T1946 |
T1945 |
nummod |
3,neurotrophin |
R641 |
T2043 |
T2044 |
det |
The,involvement |
R642 |
T1947 |
T1945 |
punct |
(,neurotrophin |
R643 |
T2044 |
T2045 |
nsubj |
involvement,raises |
R644 |
T1948 |
T1945 |
appos |
NT,neurotrophin |
R645 |
T2046 |
T2044 |
prep |
of,involvement |
R646 |
T2047 |
T2048 |
npadvmod |
target,derived |
R647 |
T2048 |
T2050 |
amod |
derived,signals |
R648 |
T1949 |
T1948 |
punct |
-,NT |
R649 |
T2049 |
T2048 |
punct |
-,derived |
R650 |
T2050 |
T2046 |
pobj |
signals,of |
R651 |
T1950 |
T1948 |
nummod |
3,NT |
R652 |
T2051 |
T2044 |
prep |
in,involvement |
R653 |
T2052 |
T2051 |
pobj |
induction,in |
R654 |
T2053 |
T2052 |
prep |
of,induction |
R655 |
T1951 |
T1941 |
punct |
),mediated |
R656 |
T2054 |
T2055 |
compound |
ETS,expression |
R657 |
T2055 |
T2053 |
pobj |
expression,of |
R658 |
T1952 |
T1953 |
punct |
[,9 |
R659 |
T2056 |
T2055 |
compound |
transcription,expression |
R660 |
T1953 |
T1941 |
parataxis |
9,mediated |
R661 |
T2057 |
T2055 |
compound |
factor,expression |
R662 |
T2058 |
T2059 |
det |
the,question |
R663 |
T1954 |
T1953 |
punct |
],9 |
R664 |
T2059 |
T2045 |
dobj |
question,raises |
R665 |
T1955 |
T1929 |
punct |
.,known |
R666 |
T2060 |
T2059 |
prep |
of,question |
R667 |
T2061 |
T2062 |
det |
the,necessity |
R668 |
T2062 |
T2060 |
pobj |
necessity,of |
R669 |
T2063 |
T2062 |
prep |
for,necessity |
R670 |
T2064 |
T2065 |
det |
the,delay |
R671 |
T2065 |
T2063 |
pobj |
delay,for |
R672 |
T1957 |
T1958 |
det |
These,proteins |
R673 |
T2066 |
T2065 |
amod |
observed,delay |
R674 |
T2067 |
T2065 |
prep |
in,delay |
R675 |
T2068 |
T2069 |
det |
the,onset |
R676 |
T2069 |
T2067 |
pobj |
onset,in |
R677 |
T1958 |
T1961 |
nsubj |
proteins,control |
R678 |
T2070 |
T2069 |
prep |
of,onset |
R679 |
T2071 |
T2072 |
compound |
ETS,signaling |
R680 |
T2072 |
T2070 |
pobj |
signaling,of |
R681 |
T1959 |
T1958 |
nummod |
two,proteins |
R682 |
T2073 |
T2072 |
prep |
for,signaling |
R683 |
T2074 |
T2075 |
amod |
neuronal,maturation |
R684 |
T1960 |
T1958 |
compound |
ETS,proteins |
R685 |
T1962 |
T1963 |
amod |
late,aspects |
R686 |
T1963 |
T1961 |
dobj |
aspects,control |
R687 |
T1964 |
T1963 |
prep |
of,aspects |
R688 |
T1965 |
T1966 |
amod |
spinal,circuit |
R689 |
T2075 |
T2073 |
pobj |
maturation,for |
R690 |
T1966 |
T1968 |
compound |
circuit,assembly |
R691 |
T2076 |
T2045 |
punct |
.,raises |
R692 |
T2078 |
T2079 |
aux |
Would,direct |
R693 |
T1967 |
T1966 |
amod |
monosynaptic,circuit |
R694 |
T2080 |
T2081 |
amod |
precocious,expression |
R695 |
T1968 |
T1964 |
pobj |
assembly,of |
R696 |
T2081 |
T2079 |
nsubj |
expression,direct |
R697 |
T2082 |
T2081 |
prep |
of,expression |
R698 |
T2182 |
T2181 |
amod |
spinal,cord |
R699 |
T2083 |
T2084 |
compound |
ETS,proteins |
R700 |
T2084 |
T2082 |
pobj |
proteins,of |
R701 |
T2085 |
T2081 |
prep |
in,expression |
R702 |
T2086 |
T2087 |
amod |
post-mitotic,neurons |
R703 |
T2087 |
T2085 |
pobj |
neurons,in |
R704 |
T2088 |
T2079 |
advmod |
also,direct |
R705 |
T2089 |
T2090 |
det |
the,programs |
R706 |
T2090 |
T2079 |
dobj |
programs,direct |
R707 |
T2183 |
T2156 |
punct |
.,find |
R708 |
T2091 |
T2090 |
amod |
appropriate,programs |
R709 |
T2092 |
T2093 |
amod |
sensory,neuron |
R710 |
T2185 |
T2186 |
amod |
Precocious,initiation |
R711 |
T2093 |
T2090 |
nmod |
neuron,programs |
R712 |
T2094 |
T2090 |
amod |
developmental,programs |
R713 |
T2095 |
T2079 |
punct |
?,direct |
R714 |
T2186 |
T2187 |
nsubj |
initiation,leads |
R715 |
T2097 |
T2098 |
prep |
In,used |
R716 |
T2188 |
T2186 |
prep |
of,initiation |
R717 |
T2099 |
T2100 |
det |
this,study |
R718 |
T2100 |
T2097 |
pobj |
study,In |
R719 |
T2101 |
T2098 |
punct |
", ",used |
R720 |
T2102 |
T2098 |
nsubj |
we,used |
R721 |
T2103 |
T2098 |
aux |
have,used |
R722 |
T2189 |
T2190 |
compound |
ETS,signaling |
R723 |
T2104 |
T2105 |
compound |
mouse,genetics |
R724 |
T2105 |
T2098 |
dobj |
genetics,used |
R725 |
T2106 |
T2107 |
aux |
to,test |
R726 |
T2190 |
T2188 |
pobj |
signaling,of |
R727 |
T2107 |
T2098 |
advcl |
test,used |
R728 |
T2108 |
T2109 |
det |
this,idea |
R729 |
T2109 |
T2107 |
dobj |
idea,test |
R730 |
T2191 |
T2190 |
prep |
in,signaling |
R731 |
T2110 |
T2109 |
amod |
general,idea |
R732 |
T2111 |
T2107 |
punct |
", ",test |
R733 |
T2192 |
T2193 |
amod |
post-mitotic,neurons |
R734 |
T2112 |
T2107 |
prep |
by,test |
R735 |
T2113 |
T2112 |
pcomp |
investigating,by |
R736 |
T2114 |
T2115 |
mark |
whether,is |
R737 |
T2193 |
T2191 |
pobj |
neurons,in |
R738 |
T2115 |
T2113 |
ccomp |
is,investigating |
R739 |
T2116 |
T2117 |
det |
the,timing |
R740 |
T2117 |
T2115 |
nsubj |
timing,is |
R741 |
T2194 |
T2193 |
compound |
DRG,neurons |
R742 |
T2118 |
T2117 |
amod |
precise,timing |
R743 |
T2119 |
T2117 |
prep |
of,timing |
R744 |
T2120 |
T2119 |
pobj |
onset,of |
R745 |
T2195 |
T2187 |
prep |
to,leads |
R746 |
T2121 |
T2120 |
prep |
of,onset |
R747 |
T2122 |
T2123 |
compound |
ETS,signaling |
R748 |
T2196 |
T2197 |
amod |
abnormal,differentiation |
R749 |
T2123 |
T2121 |
pobj |
signaling,of |
R750 |
T2124 |
T2125 |
compound |
transcription,factor |
R751 |
T2125 |
T2123 |
compound |
factor,signaling |
R752 |
T2197 |
T2195 |
pobj |
differentiation,to |
R753 |
T2126 |
T2115 |
acomp |
essential,is |
R754 |
T2127 |
T2126 |
prep |
for,essential |
R755 |
T2198 |
T2197 |
compound |
DRG,differentiation |
R756 |
T2199 |
T2197 |
compound |
neuron,differentiation |
R757 |
T2128 |
T2129 |
amod |
normal,development |
R758 |
T2129 |
T2127 |
pobj |
development,for |
R759 |
T2200 |
T2197 |
acl |
characterized,differentiation |
R760 |
T2130 |
T2131 |
amod |
sensory,neuron |
R761 |
T2131 |
T2129 |
compound |
neuron,development |
R762 |
T2201 |
T2200 |
agent |
by,characterized |
R763 |
T2132 |
T2098 |
punct |
.,used |
R764 |
T2134 |
T2135 |
nsubj |
We,assessed |
R765 |
T2202 |
T2203 |
npadvmod |
neurotrophin,independent |
R766 |
T2136 |
T2135 |
aux |
have,assessed |
R767 |
T2203 |
T2205 |
amod |
independent,outgrowth |
R768 |
T2137 |
T2138 |
det |
the,effects |
R769 |
T2138 |
T2135 |
dobj |
effects,assessed |
R770 |
T2204 |
T2203 |
punct |
-,independent |
R771 |
T2139 |
T2138 |
amod |
biological,effects |
R772 |
T2140 |
T2138 |
prep |
of,effects |
R773 |
T2141 |
T2140 |
pcomp |
inducing,of |
R774 |
T2142 |
T2143 |
compound |
ETS,signaling |
R775 |
T2143 |
T2141 |
dobj |
signaling,inducing |
R776 |
T2205 |
T2201 |
pobj |
outgrowth,by |
R777 |
T2144 |
T2145 |
preconj |
either,at |
R778 |
T2206 |
T2205 |
compound |
neurite,outgrowth |
R779 |
T2145 |
T2141 |
prep |
at,inducing |
R780 |
T2146 |
T2147 |
det |
the,time |
R781 |
T2147 |
T2145 |
pobj |
time,at |
R782 |
T2207 |
T2205 |
cc |
and,outgrowth |
R783 |
T2148 |
T2147 |
amod |
correct,time |
R784 |
T2149 |
T2147 |
amod |
developmental,time |
R785 |
T2150 |
T2145 |
punct |
", ",at |
R786 |
T2208 |
T2209 |
amod |
inappropriate,profiles |
R787 |
T2151 |
T2145 |
cc |
or,at |
R788 |
T2152 |
T2145 |
conj |
precociously,at |
R789 |
T2153 |
T2135 |
punct |
.,assessed |
R790 |
T2209 |
T2205 |
conj |
profiles,outgrowth |
R791 |
T2155 |
T2156 |
nsubj |
We,find |
R792 |
T2210 |
T2209 |
prep |
of,profiles |
R793 |
T2157 |
T2158 |
mark |
that,is |
R794 |
T2158 |
T2156 |
ccomp |
is,find |
R795 |
T2211 |
T2212 |
compound |
gene,expression |
R796 |
T2159 |
T2158 |
prep |
within,is |
R797 |
T2160 |
T2161 |
amod |
proprioceptive,neurons |
R798 |
T2161 |
T2159 |
pobj |
neurons,within |
R799 |
T2212 |
T2210 |
pobj |
expression,of |
R800 |
T2162 |
T2161 |
amod |
sensory,neurons |
R801 |
T2163 |
T2158 |
punct |
", ",is |
R802 |
T2164 |
T2165 |
det |
the,onset |
R803 |
T2213 |
T2187 |
punct |
.,leads |
R804 |
T2165 |
T2158 |
nsubj |
onset,is |
R805 |
T2166 |
T2165 |
amod |
late,onset |
R806 |
T2215 |
T2216 |
poss |
Our,findings |
R807 |
T2167 |
T2165 |
prep |
of,onset |
R808 |
T2168 |
T2169 |
compound |
ETS,signaling |
R809 |
T2169 |
T2167 |
pobj |
signaling,of |
R810 |
T2170 |
T2158 |
acomp |
essential,is |
R811 |
T2171 |
T2170 |
prep |
for,essential |
R812 |
T2172 |
T2173 |
det |
the,establishment |
R813 |
T2173 |
T2171 |
pobj |
establishment,for |
R814 |
T2216 |
T2217 |
nsubj |
findings,reveal |
R815 |
T2174 |
T2173 |
prep |
of,establishment |
R816 |
T2175 |
T2176 |
amod |
normal,projections |
R817 |
T2176 |
T2174 |
pobj |
projections,of |
R818 |
T2218 |
T2219 |
mark |
that,provide |
R819 |
T2177 |
T2178 |
amod |
sensory,afferent |
R820 |
T2178 |
T2176 |
nmod |
afferent,projections |
R821 |
T2179 |
T2173 |
prep |
in,establishment |
R822 |
T2180 |
T2181 |
det |
the,cord |
R823 |
T2219 |
T2217 |
ccomp |
provide,reveal |
R824 |
T2181 |
T2179 |
pobj |
cord,in |
R825 |
T2220 |
T2221 |
npadvmod |
target,triggered |
R826 |
T2221 |
T2223 |
amod |
triggered,signals |
R827 |
T2222 |
T2221 |
punct |
-,triggered |
R828 |
T2223 |
T2219 |
nsubj |
signals,provide |
R829 |
T2224 |
T2223 |
amod |
inductive,signals |
R830 |
T2225 |
T2226 |
det |
an,means |
R831 |
T2226 |
T2219 |
dobj |
means,provide |
R832 |
T2227 |
T2226 |
amod |
effective,means |
R833 |
T2228 |
T2226 |
prep |
of,means |
R834 |
T2229 |
T2228 |
pcomp |
ensuring,of |
R835 |
T2230 |
T2231 |
det |
the,onset |
R836 |
T2231 |
T2229 |
dobj |
onset,ensuring |
R837 |
T2232 |
T2231 |
amod |
late,onset |
R838 |
T2233 |
T2231 |
prep |
of,onset |
R839 |
T2234 |
T2233 |
pobj |
expression,of |
R840 |
T2235 |
T2234 |
prep |
of,expression |
R841 |
T2236 |
T2237 |
compound |
transcription,factors |
R842 |
T2237 |
T2235 |
pobj |
factors,of |
R843 |
T2238 |
T2219 |
punct |
", ",provide |
R844 |
T2239 |
T2219 |
cc |
and,provide |
R845 |
T2240 |
T2241 |
advmod |
thus,sequence |
R846 |
T2241 |
T2219 |
conj |
sequence,provide |
R847 |
T2242 |
T2241 |
det |
an,sequence |
R848 |
T2243 |
T2241 |
amod |
orderly,sequence |
R849 |
T2244 |
T2241 |
amod |
temporal,sequence |
R850 |
T2245 |
T2241 |
amod |
transcriptional,sequence |
R851 |
T2246 |
T2247 |
dep |
that,is |
R852 |
T2247 |
T2241 |
relcl |
is,sequence |
R853 |
T2248 |
T2247 |
acomp |
crucial,is |
R854 |
T2249 |
T2248 |
prep |
for,crucial |
R855 |
T2250 |
T2251 |
amod |
neuronal,maturation |
R856 |
T2251 |
T2249 |
pobj |
maturation,for |
R857 |
T2252 |
T2251 |
cc |
and,maturation |
R858 |
T2253 |
T2254 |
compound |
circuit,assembly |
R859 |
T2254 |
T2251 |
conj |
assembly,maturation |
R860 |
T2255 |
T2217 |
punct |
.,reveal |
R864 |
T2468 |
T2469 |
aux |
To,test |
R865 |
T2469 |
T2470 |
advcl |
test,studied |
R866 |
T2471 |
T2472 |
det |
the,hypothesis |
R867 |
T2472 |
T2469 |
dobj |
hypothesis,test |
R868 |
T2473 |
T2474 |
mark |
that,is |
R869 |
T2474 |
T2472 |
acl |
is,hypothesis |
R870 |
T2475 |
T2476 |
det |
a,delay |
R871 |
T2476 |
T2474 |
nsubj |
delay,is |
R872 |
T2477 |
T2476 |
amod |
temporal,delay |
R873 |
T2478 |
T2476 |
prep |
in,delay |
R874 |
T2479 |
T2480 |
det |
the,onset |
R875 |
T2480 |
T2478 |
pobj |
onset,in |
R876 |
T2481 |
T2480 |
prep |
of,onset |
R877 |
T2482 |
T2483 |
amod |
transcriptional,programs |
R878 |
T2483 |
T2481 |
pobj |
programs,of |
R879 |
T2484 |
T2474 |
acomp |
crucial,is |
R880 |
T2485 |
T2484 |
prep |
for,crucial |
R881 |
T2486 |
T2487 |
det |
the,control |
R882 |
T2487 |
T2485 |
pobj |
control,for |
R883 |
T2488 |
T2487 |
prep |
of,control |
R884 |
T2489 |
T2490 |
amod |
appropriate,maturation |
R885 |
T2490 |
T2488 |
pobj |
maturation,of |
R886 |
T2491 |
T2490 |
amod |
neuronal,maturation |
R887 |
T2492 |
T2470 |
punct |
", ",studied |
R888 |
T2493 |
T2470 |
nsubj |
we,studied |
R889 |
T2494 |
T2495 |
det |
the,development |
R890 |
T2495 |
T2470 |
dobj |
development,studied |
R891 |
T2496 |
T2495 |
prep |
of,development |
R892 |
T2497 |
T2498 |
amod |
proprioceptive,neurons |
R893 |
T2498 |
T2496 |
pobj |
neurons,of |
R894 |
T2499 |
T2498 |
compound |
DRG,neurons |
R895 |
T2500 |
T2470 |
punct |
", ",studied |
R896 |
T2501 |
T2502 |
mark |
since,identified |
R897 |
T2502 |
T2470 |
advcl |
identified,studied |
R898 |
T2503 |
T2504 |
amod |
transcriptional,effectors |
R899 |
T2504 |
T2502 |
nsubjpass |
effectors,identified |
R900 |
T2505 |
T2504 |
acl |
regulated,effectors |
R901 |
T2506 |
T2505 |
agent |
by,regulated |
R902 |
T2507 |
T2508 |
npadvmod |
target,derived |
R903 |
T2508 |
T2510 |
amod |
derived,signals |
R904 |
T2509 |
T2508 |
punct |
-,derived |
R905 |
T2510 |
T2506 |
pobj |
signals,by |
R906 |
T2511 |
T2510 |
punct |
", ",signals |
R907 |
T2512 |
T2513 |
advmod |
as,as |
R908 |
T2513 |
T2510 |
cc |
as,signals |
R909 |
T2514 |
T2513 |
advmod |
well,as |
R910 |
T2515 |
T2510 |
conj |
some,signals |
R911 |
T2516 |
T2515 |
prep |
of,some |
R912 |
T2517 |
T2518 |
poss |
their,actions |
R913 |
T2518 |
T2516 |
pobj |
actions,of |
R914 |
T2519 |
T2518 |
amod |
downstream,actions |
R915 |
T2520 |
T2518 |
amod |
biological,actions |
R916 |
T2521 |
T2502 |
punct |
", ",identified |
R917 |
T2522 |
T2502 |
aux |
have,identified |
R918 |
T2523 |
T2502 |
auxpass |
been,identified |
R919 |
T2524 |
T2502 |
prep |
for,identified |
R920 |
T2525 |
T2526 |
det |
these,neurons |
R921 |
T2526 |
T2524 |
pobj |
neurons,for |
R922 |
T2527 |
T2470 |
punct |
.,studied |
R923 |
T2529 |
T2530 |
nsubj |
Er81,controls |
R924 |
T2531 |
T2532 |
amod |
proprioceptive,connectivity |
R925 |
T2532 |
T2530 |
dobj |
connectivity,controls |
R926 |
T2533 |
T2532 |
amod |
afferent,connectivity |
R927 |
T2534 |
T2535 |
punct |
[,14 |
R928 |
T2535 |
T2530 |
parataxis |
14,controls |
R929 |
T2536 |
T2535 |
punct |
],14 |
R930 |
T2537 |
T2530 |
punct |
", ",controls |
R931 |
T2538 |
T2530 |
cc |
and,controls |
R932 |
T2539 |
T2540 |
nsubj |
we,sought |
R933 |
T2540 |
T2530 |
conj |
sought,controls |
R934 |
T2541 |
T2540 |
advmod |
therefore,sought |
R935 |
T2542 |
T2543 |
aux |
to,identify |
R936 |
T2543 |
T2540 |
xcomp |
identify,sought |
R937 |
T2544 |
T2545 |
det |
an,regulator |
R938 |
T2545 |
T2543 |
dobj |
regulator,identify |
R939 |
T2546 |
T2545 |
nmod |
ETS,regulator |
R940 |
T2547 |
T2545 |
amod |
transcriptional,regulator |
R941 |
T2548 |
T2549 |
dep |
that,is |
R942 |
T2549 |
T2545 |
relcl |
is,regulator |
R943 |
T2550 |
T2549 |
punct |
", ",is |
R944 |
T2551 |
T2552 |
advmod |
when,expressed |
R945 |
T2552 |
T2549 |
advcl |
expressed,is |
R946 |
T2553 |
T2552 |
prep |
over,expressed |
R947 |
T2554 |
T2555 |
det |
the,course |
R948 |
T2555 |
T2553 |
pobj |
course,over |
R949 |
T2556 |
T2555 |
amod |
normal,course |
R950 |
T2557 |
T2555 |
compound |
time,course |
R951 |
T2558 |
T2555 |
prep |
of,course |
R952 |
T2559 |
T2560 |
compound |
Er81,expression |
R953 |
T2560 |
T2558 |
pobj |
expression,of |
R954 |
T2561 |
T2549 |
punct |
", ",is |
R955 |
T2562 |
T2549 |
acomp |
able,is |
R956 |
T2563 |
T2564 |
aux |
to,substitute |
R957 |
T2564 |
T2562 |
xcomp |
substitute,able |
R958 |
T2565 |
T2564 |
prep |
for,substitute |
R959 |
T2566 |
T2567 |
compound |
Er81,function |
R960 |
T2567 |
T2565 |
pobj |
function,for |
R961 |
T2568 |
T2564 |
prep |
within,substitute |
R962 |
T2569 |
T2570 |
nmod |
group,Ia |
R963 |
T2570 |
T2571 |
nmod |
Ia,neurons |
R964 |
T2571 |
T2568 |
pobj |
neurons,within |
R965 |
T2572 |
T2571 |
amod |
afferent,neurons |
R966 |
T2573 |
T2571 |
amod |
sensory,neurons |
R967 |
T2574 |
T2540 |
punct |
.,sought |
R968 |
T2576 |
T2577 |
prep |
With,designed |
R969 |
T2578 |
T2579 |
det |
this,point |
R970 |
T2579 |
T2576 |
pobj |
point,With |
R971 |
T2580 |
T2579 |
compound |
reference,point |
R972 |
T2581 |
T2577 |
punct |
", ",designed |
R973 |
T2582 |
T2577 |
nsubj |
we,designed |
R974 |
T2583 |
T2577 |
advmod |
then,designed |
R975 |
T2584 |
T2577 |
dobj |
experiments,designed |
R976 |
T2585 |
T2586 |
aux |
to,examine |
R977 |
T2586 |
T2584 |
advcl |
examine,experiments |
R978 |
T2587 |
T2588 |
det |
the,effects |
R979 |
T2588 |
T2586 |
dobj |
effects,examine |
R980 |
T2589 |
T2588 |
prep |
of,effects |
R981 |
T2590 |
T2591 |
amod |
precocious,expression |
R982 |
T2591 |
T2589 |
pobj |
expression,of |
R983 |
T2592 |
T2591 |
amod |
post-mitotic,expression |
R984 |
T2593 |
T2591 |
prep |
of,expression |
R985 |
T2594 |
T2595 |
det |
the,factor |
R986 |
T2595 |
T2593 |
pobj |
factor,of |
R987 |
T2596 |
T2595 |
amod |
same,factor |
R988 |
T2597 |
T2595 |
compound |
ETS,factor |
R989 |
T2598 |
T2595 |
compound |
transcription,factor |
R990 |
T2599 |
T2588 |
prep |
on,effects |
R991 |
T2600 |
T2601 |
amod |
sensory,neuron |
R992 |
T2601 |
T2602 |
compound |
neuron,differentiation |
R993 |
T2602 |
T2599 |
pobj |
differentiation,on |
R994 |
T2603 |
T2577 |
punct |
.,designed |
R999 |
T3609 |
T3610 |
compound |
EWS,Pea3 |
R1000 |
T3610 |
T3612 |
nsubj |
Pea3,Replace |
R1001 |
T3611 |
T3610 |
punct |
-,Pea3 |
R1002 |
T3613 |
T3612 |
aux |
Can,Replace |
R1003 |
T3614 |
T3615 |
compound |
Er81,Function |
R1004 |
T3615 |
T3612 |
dobj |
Function,Replace |
R1005 |
T3616 |
T3612 |
prep |
in,Replace |
R1006 |
T3617 |
T3616 |
pcomp |
Controlling,in |
R1007 |
T3618 |
T3619 |
nmod |
Ia,Projections |
R1008 |
T3619 |
T3617 |
dobj |
Projections,Controlling |
R1009 |
T3620 |
T3619 |
amod |
Afferent,Projections |
R1010 |
T3622 |
T3623 |
nsubj |
We,defined |
R1011 |
T3624 |
T3623 |
advmod |
first,defined |
R1012 |
T3625 |
T3626 |
det |
an,regulator |
R1013 |
T3626 |
T3623 |
dobj |
regulator,defined |
R1014 |
T3627 |
T3626 |
compound |
ETS,regulator |
R1015 |
T3628 |
T3626 |
compound |
transcription,regulator |
R1016 |
T3629 |
T3630 |
dep |
that,is |
R1017 |
T3630 |
T3626 |
relcl |
is,regulator |
R1018 |
T3631 |
T3630 |
acomp |
able,is |
R1019 |
T3632 |
T3633 |
aux |
to,replace |
R1020 |
T3633 |
T3631 |
xcomp |
replace,able |
R1021 |
T3634 |
T3635 |
det |
the,function |
R1022 |
T3635 |
T3633 |
dobj |
function,replace |
R1023 |
T3636 |
T3635 |
prep |
of,function |
R1024 |
T3637 |
T3636 |
pobj |
Er81,of |
R1025 |
T3638 |
T3635 |
prep |
within,function |
R1026 |
T3639 |
T3640 |
amod |
proprioceptive,afferents |
R1027 |
T3640 |
T3638 |
pobj |
afferents,within |
R1028 |
T3641 |
T3642 |
aux |
to,direct |
R1029 |
T3642 |
T3633 |
advcl |
direct,replace |
R1030 |
T3643 |
T3642 |
dobj |
projections,direct |
R1031 |
T3644 |
T3642 |
prep |
into,direct |
R1032 |
T3645 |
T3646 |
det |
the,cord |
R1033 |
T3646 |
T3644 |
pobj |
cord,into |
R1034 |
T3647 |
T3646 |
amod |
ventral,cord |
R1035 |
T3648 |
T3646 |
amod |
spinal,cord |
R1036 |
T3649 |
T3623 |
punct |
.,defined |
R1037 |
T3651 |
T3652 |
nsubj |
Er81,constitute |
R1038 |
T3653 |
T3651 |
punct |
", ",Er81 |
R1039 |
T3654 |
T3651 |
conj |
Pea3,Er81 |
R1040 |
T3655 |
T3654 |
punct |
", ",Pea3 |
R1041 |
T3656 |
T3654 |
cc |
and,Pea3 |
R1042 |
T3657 |
T3654 |
conj |
Erm,Pea3 |
R1043 |
T3658 |
T3659 |
det |
the,subfamily |
R1044 |
T3659 |
T3652 |
dobj |
subfamily,constitute |
R1045 |
T3660 |
T3659 |
compound |
Pea3,subfamily |
R1046 |
T3661 |
T3659 |
prep |
of,subfamily |
R1047 |
T3662 |
T3663 |
compound |
ETS,factors |
R1048 |
T3663 |
T3661 |
pobj |
factors,of |
R1049 |
T3664 |
T3663 |
compound |
transcription,factors |
R1050 |
T3665 |
T3652 |
punct |
", ",constitute |
R1051 |
T3666 |
T3652 |
conj |
show,constitute |
R1052 |
T3667 |
T3668 |
det |
a,degree |
R1053 |
T3668 |
T3666 |
dobj |
degree,show |
R1054 |
T3669 |
T3668 |
amod |
high,degree |
R1055 |
T3670 |
T3668 |
prep |
of,degree |
R1056 |
T3671 |
T3672 |
compound |
amino,acid |
R1057 |
T3672 |
T3673 |
compound |
acid,identity |
R1058 |
T3673 |
T3670 |
pobj |
identity,of |
R1059 |
T3674 |
T3666 |
punct |
", ",show |
R1060 |
T3675 |
T3666 |
cc |
and,show |
R1061 |
T3676 |
T3666 |
conj |
bind,show |
R1062 |
T3677 |
T3676 |
prep |
to,bind |
R1063 |
T3678 |
T3679 |
advmod |
very,similar |
R1064 |
T3679 |
T3680 |
amod |
similar,sequences |
R1065 |
T3680 |
T3677 |
pobj |
sequences,to |
R1066 |
T3681 |
T3680 |
compound |
DNA,sequences |
R1067 |
T3682 |
T3680 |
compound |
target,sequences |
R1068 |
T3683 |
T3684 |
punct |
[,19 |
R1069 |
T3684 |
T3676 |
parataxis |
19,bind |
R1070 |
T3685 |
T3684 |
nummod |
17,19 |
R1071 |
T3686 |
T3684 |
punct |
",",19 |
R1072 |
T3687 |
T3684 |
nummod |
18,19 |
R1073 |
T3688 |
T3684 |
punct |
",",19 |
R1074 |
T3689 |
T3684 |
punct |
],19 |
R1075 |
T3690 |
T3652 |
punct |
.,constitute |
R1076 |
T3692 |
T3693 |
advmod |
Nevertheless,rescue |
R1077 |
T3694 |
T3693 |
punct |
", ",rescue |
R1078 |
T3695 |
T3696 |
advmod |
when,introduced |
R1079 |
T3696 |
T3693 |
advcl |
introduced,rescue |
R1080 |
T3697 |
T3696 |
prep |
into,introduced |
R1081 |
T3698 |
T3699 |
det |
the,locus |
R1082 |
T3699 |
T3697 |
pobj |
locus,into |
R1083 |
T3700 |
T3699 |
compound |
Er81,locus |
R1084 |
T3701 |
T3696 |
prep |
in,introduced |
R1085 |
T3702 |
T3701 |
pobj |
analogy,in |
R1086 |
T3703 |
T3702 |
prep |
to,analogy |
R1087 |
T3704 |
T3705 |
det |
a,strategy |
R1088 |
T3705 |
T3703 |
pobj |
strategy,to |
R1089 |
T3706 |
T3707 |
advmod |
previously,used |
R1090 |
T3707 |
T3705 |
amod |
used,strategy |
R1091 |
T3708 |
T3705 |
compound |
targeting,strategy |
R1092 |
T3709 |
T3710 |
punct |
(,shown |
R1093 |
T3710 |
T3696 |
parataxis |
shown,introduced |
R1094 |
T3711 |
T3710 |
nsubj |
data,shown |
R1095 |
T3712 |
T3710 |
neg |
not,shown |
R1096 |
T3713 |
T3710 |
punct |
;,shown |
R1097 |
T3714 |
T3715 |
punct |
[,14 |
R1098 |
T3715 |
T3710 |
parataxis |
14,shown |
R1099 |
T3716 |
T3715 |
punct |
],14 |
R1100 |
T3717 |
T3710 |
punct |
),shown |
R1101 |
T3718 |
T3693 |
punct |
", ",rescue |
R1102 |
T3719 |
T3720 |
preconj |
neither,Pea3 |
R1103 |
T3720 |
T3693 |
nsubj |
Pea3,rescue |
R1104 |
T3721 |
T3720 |
cc |
nor,Pea3 |
R1105 |
T3722 |
T3720 |
conj |
Erm,Pea3 |
R1106 |
T3723 |
T3693 |
aux |
could,rescue |
R1107 |
T3724 |
T3725 |
npadvmod |
Ia,proprioceptive |
R1108 |
T3725 |
T3726 |
amod |
proprioceptive,projections |
R1109 |
T3726 |
T3693 |
dobj |
projections,rescue |
R1110 |
T3727 |
T3726 |
amod |
afferent,projections |
R1111 |
T3728 |
T3729 |
aux |
to,invade |
R1112 |
T3729 |
T3693 |
advcl |
invade,rescue |
R1113 |
T3730 |
T3729 |
advmod |
extensively,invade |
R1114 |
T3731 |
T3732 |
det |
the,horn |
R1115 |
T3732 |
T3729 |
dobj |
horn,invade |
R1116 |
T3733 |
T3732 |
amod |
ventral,horn |
R1117 |
T3734 |
T3732 |
prep |
of,horn |
R1118 |
T3735 |
T3736 |
det |
the,cord |
R1119 |
T3736 |
T3734 |
pobj |
cord,of |
R1120 |
T3737 |
T3736 |
amod |
spinal,cord |
R1121 |
T3738 |
T3739 |
punct |
(,shown |
R1122 |
T3739 |
T3729 |
parataxis |
shown,invade |
R1123 |
T3740 |
T3739 |
nsubj |
data,shown |
R1124 |
T3741 |
T3739 |
neg |
not,shown |
R1125 |
T3742 |
T3739 |
punct |
),shown |
R1126 |
T3743 |
T3693 |
punct |
.,rescue |
R1127 |
T3745 |
T3746 |
det |
These,findings |
R1128 |
T3746 |
T3747 |
nsubj |
findings,prompted |
R1129 |
T3748 |
T3747 |
dobj |
us,prompted |
R1130 |
T3749 |
T3750 |
aux |
to,analyze |
R1131 |
T3750 |
T3747 |
xcomp |
analyze,prompted |
R1132 |
T3751 |
T3750 |
dobj |
mice,analyze |
R1133 |
T3752 |
T3753 |
prep |
in,integrated |
R1134 |
T3753 |
T3751 |
relcl |
integrated,mice |
R1135 |
T3754 |
T3752 |
pobj |
which,in |
R1136 |
T3755 |
T3753 |
nsubj |
we,integrated |
R1137 |
T3756 |
T3757 |
compound |
EWS,Pea3 |
R1138 |
T3757 |
T3753 |
dobj |
Pea3,integrated |
R1139 |
T3758 |
T3757 |
punct |
-,Pea3 |
R1140 |
T3759 |
T3757 |
punct |
", ",Pea3 |
R1141 |
T3760 |
T3761 |
det |
a,product |
R1142 |
T3761 |
T3757 |
appos |
product,Pea3 |
R1143 |
T3762 |
T3763 |
compound |
break,point |
R1144 |
T3763 |
T3761 |
compound |
point,product |
R1145 |
T3764 |
T3763 |
punct |
-,point |
R1146 |
T3765 |
T3761 |
compound |
fusion,product |
R1147 |
T3766 |
T3761 |
prep |
between,product |
R1148 |
T3767 |
T3768 |
det |
the,domain |
R1149 |
T3768 |
T3766 |
pobj |
domain,between |
R1150 |
T3769 |
T3770 |
npadvmod |
amino,terminal |
R1151 |
T3770 |
T3768 |
amod |
terminal,domain |
R1152 |
T3771 |
T3770 |
punct |
-,terminal |
R1153 |
T3772 |
T3768 |
prep |
of,domain |
R1154 |
T3773 |
T3774 |
det |
the,gene |
R1155 |
T3774 |
T3772 |
pobj |
gene,of |
R1156 |
T3775 |
T3776 |
nmod |
Ewing,sarcoma |
R1157 |
T3776 |
T3774 |
nmod |
sarcoma,gene |
R1158 |
T3777 |
T3776 |
punct |
(,sarcoma |
R1159 |
T3778 |
T3776 |
appos |
EWS,sarcoma |
R1160 |
T3779 |
T3774 |
punct |
),gene |
R1161 |
T3780 |
T3768 |
cc |
and,domain |
R1162 |
T3781 |
T3782 |
det |
the,domain |
R1163 |
T3782 |
T3768 |
conj |
domain,domain |
R1164 |
T3783 |
T3784 |
compound |
Pea3,DNA |
R1165 |
T3784 |
T3785 |
npadvmod |
DNA,binding |
R1166 |
T3785 |
T3782 |
amod |
binding,domain |
R1167 |
T3786 |
T3787 |
punct |
[,21 |
R1168 |
T3787 |
T3782 |
parataxis |
21,domain |
R1169 |
T3788 |
T3787 |
nummod |
20,21 |
R1170 |
T3789 |
T3787 |
punct |
",",21 |
R1171 |
T3790 |
T3787 |
punct |
],21 |
R1172 |
T3791 |
T3753 |
punct |
", ",integrated |
R1173 |
T3792 |
T3753 |
prep |
into,integrated |
R1174 |
T3793 |
T3794 |
det |
the,locus |
R1175 |
T3794 |
T3792 |
pobj |
locus,into |
R1176 |
T3795 |
T3794 |
compound |
Er81,locus |
R1177 |
T3796 |
T3797 |
punct |
(,Figure |
R1178 |
T3797 |
T3753 |
parataxis |
Figure,integrated |
R1179 |
T3798 |
T3797 |
nummod |
1,Figure |
R1180 |
T3799 |
T3797 |
punct |
),Figure |
R1181 |
T3800 |
T3747 |
punct |
.,prompted |
R1182 |
T3802 |
T3803 |
nsubj |
We,found |
R1183 |
T3804 |
T3805 |
mark |
that,showed |
R1184 |
T3805 |
T3803 |
ccomp |
showed,found |
R1185 |
T3806 |
T3805 |
prep |
in,showed |
R1186 |
T3807 |
T3808 |
det |
a,assay |
R1187 |
T3808 |
T3806 |
pobj |
assay,in |
R1188 |
T3809 |
T3810 |
compound |
luciferase,enzyme |
R1189 |
T3810 |
T3812 |
npadvmod |
enzyme,based |
R1190 |
T3811 |
T3810 |
punct |
-,enzyme |
R1191 |
T3812 |
T3808 |
amod |
based,assay |
R1192 |
T3813 |
T3812 |
punct |
-,based |
R1193 |
T3814 |
T3815 |
compound |
cell,culture |
R1194 |
T3815 |
T3808 |
compound |
culture,assay |
R1195 |
T3816 |
T3808 |
compound |
transfection,assay |
R1196 |
T3817 |
T3805 |
punct |
", ",showed |
R1197 |
T3818 |
T3819 |
compound |
EWS,Pea3 |
R1198 |
T3819 |
T3805 |
nsubj |
Pea3,showed |
R1199 |
T3820 |
T3819 |
punct |
-,Pea3 |
R1200 |
T3821 |
T3822 |
amod |
stronger,activity |
R1201 |
T3822 |
T3805 |
dobj |
activity,showed |
R1202 |
T3823 |
T3822 |
compound |
transactivation,activity |
R1203 |
T3824 |
T3822 |
prep |
than,activity |
R1204 |
T3825 |
T3824 |
pobj |
Er81,than |
R1205 |
T3826 |
T3825 |
cc |
or,Er81 |
R1206 |
T3827 |
T3825 |
conj |
Pea3,Er81 |
R1207 |
T3828 |
T3829 |
punct |
(,1J |
R1208 |
T3829 |
T3822 |
parataxis |
1J,activity |
R1209 |
T3830 |
T3829 |
compound |
Figure,1J |
R1210 |
T3831 |
T3829 |
punct |
;,1J |
R1211 |
T3832 |
T3833 |
nsubj |
data,shown |
R1212 |
T3833 |
T3829 |
ccomp |
shown,1J |
R1213 |
T3834 |
T3833 |
neg |
not,shown |
R1214 |
T3835 |
T3829 |
punct |
),1J |
R1215 |
T3836 |
T3805 |
punct |
", ",showed |
R1216 |
T3837 |
T3805 |
prep |
in,showed |
R1217 |
T3838 |
T3837 |
pobj |
agreement,in |
R1218 |
T3839 |
T3838 |
prep |
with,agreement |
R1219 |
T3840 |
T3841 |
amod |
previous,studies |
R1220 |
T3841 |
T3839 |
pobj |
studies,with |
R1221 |
T3842 |
T3843 |
punct |
[,24 |
R1222 |
T3843 |
T3841 |
parataxis |
24,studies |
R1223 |
T3844 |
T3843 |
nummod |
22,24 |
R1224 |
T3845 |
T3843 |
punct |
",",24 |
R1225 |
T3846 |
T3843 |
nummod |
23,24 |
R1226 |
T3847 |
T3843 |
punct |
",",24 |
R1227 |
T3848 |
T3843 |
punct |
],24 |
R1228 |
T3849 |
T3803 |
punct |
.,found |
R1229 |
T3851 |
T3852 |
advmod |
Moreover,abolished |
R1230 |
T3853 |
T3852 |
punct |
", ",abolished |
R1231 |
T3854 |
T3852 |
nsubjpass |
transactivation,abolished |
R1232 |
T3855 |
T3854 |
prep |
by,transactivation |
R1233 |
T3856 |
T3857 |
compound |
EWS,Pea3 |
R1234 |
T3857 |
T3855 |
pobj |
Pea3,by |
R1235 |
T3858 |
T3857 |
punct |
-,Pea3 |
R1236 |
T3859 |
T3852 |
auxpass |
was,abolished |
R1237 |
T3860 |
T3852 |
agent |
by,abolished |
R1238 |
T3861 |
T3860 |
pobj |
mutation,by |
R1239 |
T3862 |
T3861 |
prep |
of,mutation |
R1240 |
T3863 |
T3864 |
npadvmod |
ETS,binding |
R1241 |
T3864 |
T3866 |
amod |
binding,sites |
R1242 |
T3865 |
T3864 |
punct |
-,binding |
R1243 |
T3866 |
T3862 |
pobj |
sites,of |
R1244 |
T3867 |
T3861 |
prep |
in,mutation |
R1245 |
T3868 |
T3869 |
det |
the,plasmid |
R1246 |
T3869 |
T3867 |
pobj |
plasmid,in |
R1247 |
T3870 |
T3869 |
compound |
reporter,plasmid |
R1248 |
T3871 |
T3852 |
punct |
", ",abolished |
R1249 |
T3872 |
T3852 |
advcl |
demonstrating,abolished |
R1250 |
T3873 |
T3874 |
npadvmod |
ETS,binding |
R1251 |
T3874 |
T3876 |
amod |
binding,site |
R1252 |
T3875 |
T3874 |
punct |
-,binding |
R1253 |
T3876 |
T3878 |
compound |
site,dependence |
R1254 |
T3877 |
T3876 |
punct |
-,site |
R1255 |
T3878 |
T3872 |
dobj |
dependence,demonstrating |
R1256 |
T3879 |
T3880 |
punct |
(,shown |
R1257 |
T3880 |
T3872 |
parataxis |
shown,demonstrating |
R1258 |
T3881 |
T3880 |
nsubj |
data,shown |
R1259 |
T3882 |
T3880 |
neg |
not,shown |
R1260 |
T3883 |
T3880 |
punct |
),shown |
R1261 |
T3884 |
T3852 |
punct |
.,abolished |
R1262 |
T3886 |
T3887 |
nsubjpass |
Expression,abolished |
R1263 |
T3888 |
T3886 |
prep |
of,Expression |
R1264 |
T3889 |
T3888 |
pobj |
Er81,of |
R1265 |
T3890 |
T3886 |
prep |
in,Expression |
R1266 |
T3891 |
T3892 |
compound |
DRG,neurons |
R1267 |
T3892 |
T3890 |
pobj |
neurons,in |
R1268 |
T3893 |
T3892 |
prep |
of,neurons |
R1269 |
T3894 |
T3893 |
pobj |
embryos,of |
R1270 |
T3895 |
T3894 |
acl |
containing,embryos |
R1271 |
T3896 |
T3895 |
dobj |
integration,containing |
R1272 |
T3897 |
T3896 |
prep |
of,integration |
R1273 |
T3898 |
T3899 |
compound |
EWS,Pea3 |
R1274 |
T3899 |
T3897 |
pobj |
Pea3,of |
R1275 |
T3900 |
T3899 |
punct |
-,Pea3 |
R1276 |
T3901 |
T3886 |
prep |
in,Expression |
R1277 |
T3902 |
T3903 |
det |
the,locus |
R1278 |
T3903 |
T3901 |
pobj |
locus,in |
R1279 |
T3904 |
T3903 |
compound |
Er81,locus |
R1280 |
T3905 |
T3903 |
punct |
(,locus |
R1281 |
T3906 |
T3907 |
compound |
Er81EWS,Pea3 |
R1282 |
T3907 |
T3903 |
appos |
Pea3,locus |
R1283 |
T3908 |
T3907 |
punct |
-,Pea3 |
R1284 |
T3909 |
T3907 |
punct |
/,Pea3 |
R1285 |
T3910 |
T3907 |
punct |
−,Pea3 |
R1286 |
T3911 |
T3887 |
punct |
),abolished |
R1287 |
T3912 |
T3887 |
auxpass |
was,abolished |
R1288 |
T3913 |
T3914 |
punct |
(,1E |
R1289 |
T3914 |
T3887 |
parataxis |
1E,abolished |
R1290 |
T3915 |
T3914 |
compound |
Figure,1E |
R1291 |
T3916 |
T3914 |
punct |
),1E |
R1292 |
T3917 |
T3887 |
punct |
", ",abolished |
R1293 |
T3918 |
T3887 |
cc |
and,abolished |
R1294 |
T3919 |
T3920 |
det |
the,level |
R1295 |
T3920 |
T3922 |
nsubj |
level,was |
R1296 |
T3921 |
T3920 |
compound |
expression,level |
R1297 |
T3922 |
T3887 |
conj |
was,abolished |
R1298 |
T3923 |
T3920 |
prep |
of,level |
R1299 |
T3924 |
T3925 |
det |
the,protein |
R1300 |
T3925 |
T3923 |
pobj |
protein,of |
R1301 |
T3926 |
T3927 |
npadvmod |
calcium,binding |
R1302 |
T3927 |
T3925 |
amod |
binding,protein |
R1303 |
T3928 |
T3927 |
punct |
-,binding |
R1304 |
T3929 |
T3925 |
appos |
Parvalbumin,protein |
R1305 |
T3930 |
T3929 |
punct |
(,Parvalbumin |
R1306 |
T3931 |
T3929 |
appos |
PV,Parvalbumin |
R1307 |
T3932 |
T3920 |
punct |
),level |
R1308 |
T3933 |
T3920 |
prep |
in,level |
R1309 |
T3934 |
T3935 |
amod |
proprioceptive,afferents |
R1310 |
T3935 |
T3933 |
pobj |
afferents,in |
R1311 |
T3936 |
T3920 |
punct |
", ",level |
R1312 |
T3937 |
T3938 |
dep |
which,decreased |
R1313 |
T3938 |
T3920 |
relcl |
decreased,level |
R1314 |
T3939 |
T3938 |
auxpass |
is,decreased |
R1315 |
T3940 |
T3941 |
advmod |
approximately,10 |
R1316 |
T3941 |
T3945 |
quantmod |
10,fold |
R1317 |
T3942 |
T3941 |
quantmod |
5,10 |
R1318 |
T3943 |
T3941 |
punct |
-,10 |
R1319 |
T3944 |
T3941 |
quantmod |
to,10 |
R1320 |
T3945 |
T3938 |
advmod |
fold,decreased |
R1321 |
T3946 |
T3945 |
punct |
-,fold |
R1322 |
T3947 |
T3938 |
prep |
in,decreased |
R1323 |
T3948 |
T3949 |
compound |
Er81,mutants |
R1324 |
T3949 |
T3947 |
pobj |
mutants,in |
R1325 |
T3950 |
T3951 |
punct |
[,14 |
R1326 |
T3951 |
T3938 |
parataxis |
14,decreased |
R1327 |
T3952 |
T3951 |
punct |
],14 |
R1328 |
T3953 |
T3922 |
punct |
", ",was |
R1329 |
T3954 |
T3922 |
acomp |
comparable,was |
R1330 |
T3955 |
T3954 |
prep |
to,comparable |
R1331 |
T3956 |
T3957 |
amod |
wild,type |
R1332 |
T3957 |
T3959 |
compound |
type,levels |
R1333 |
T3958 |
T3957 |
punct |
-,type |
R1334 |
T3959 |
T3955 |
pobj |
levels,to |
R1335 |
T3960 |
T3922 |
prep |
in,was |
R1336 |
T3961 |
T3962 |
nmod |
Er81EWS,Pea3 |
R1337 |
T3962 |
T3964 |
nmod |
Pea3,embryos |
R1338 |
T3963 |
T3962 |
punct |
-,Pea3 |
R1339 |
T3964 |
T3960 |
pobj |
embryos,in |
R1340 |
T3965 |
T3962 |
punct |
/,Pea3 |
R1341 |
T3966 |
T3962 |
punct |
−,Pea3 |
R1342 |
T3967 |
T3968 |
punct |
(,1F |
R1343 |
T3968 |
T3922 |
parataxis |
1F,was |
R1344 |
T3969 |
T3968 |
compound |
Figure,1F |
R1345 |
T3970 |
T3971 |
punct |
–,1H |
R1346 |
T3971 |
T3968 |
prep |
1H,1F |
R1347 |
T3972 |
T3968 |
punct |
),1F |
R1348 |
T3973 |
T3922 |
punct |
.,was |
R1349 |
T3975 |
T3976 |
aux |
To,define |
R1350 |
T3976 |
T3978 |
advcl |
define,assessed |
R1351 |
T3977 |
T3976 |
advmod |
further,define |
R1352 |
T3979 |
T3980 |
compound |
DRG,neuron |
R1353 |
T3980 |
T3981 |
compound |
neuron,differentiation |
R1354 |
T3981 |
T3976 |
dobj |
differentiation,define |
R1355 |
T3982 |
T3976 |
prep |
in,define |
R1356 |
T3983 |
T3984 |
det |
the,presence |
R1357 |
T3984 |
T3982 |
pobj |
presence,in |
R1358 |
T3985 |
T3984 |
prep |
of,presence |
R1359 |
T3986 |
T3987 |
compound |
EWS,Pea3 |
R1360 |
T3987 |
T3985 |
pobj |
Pea3,of |
R1361 |
T3988 |
T3987 |
punct |
-,Pea3 |
R1362 |
T3989 |
T3976 |
prep |
in,define |
R1363 |
T3990 |
T3991 |
amod |
proprioceptive,afferents |
R1364 |
T3991 |
T3989 |
pobj |
afferents,in |
R1365 |
T3992 |
T3993 |
advmod |
in,vivo |
R1366 |
T3993 |
T3976 |
advmod |
vivo,define |
R1367 |
T3994 |
T3978 |
punct |
", ",assessed |
R1368 |
T3995 |
T3978 |
nsubj |
we,assessed |
R1369 |
T3996 |
T3997 |
mark |
whether,had |
R1370 |
T3997 |
T3978 |
ccomp |
had,assessed |
R1371 |
T3998 |
T3997 |
nsubj |
replacement,had |
R1372 |
T3999 |
T3998 |
prep |
of,replacement |
R1373 |
T4000 |
T3999 |
pobj |
Er81,of |
R1374 |
T4001 |
T3998 |
prep |
by,replacement |
R1375 |
T4002 |
T4003 |
compound |
EWS,Pea3 |
R1376 |
T4003 |
T4001 |
pobj |
Pea3,by |
R1377 |
T4004 |
T4003 |
punct |
-,Pea3 |
R1378 |
T4005 |
T4006 |
det |
an,influence |
R1379 |
T4006 |
T3997 |
dobj |
influence,had |
R1380 |
T4007 |
T4006 |
prep |
on,influence |
R1381 |
T4008 |
T4009 |
amod |
neuronal,survival |
R1382 |
T4009 |
T4007 |
pobj |
survival,on |
R1383 |
T4010 |
T4007 |
cc |
or,on |
R1384 |
T4011 |
T4007 |
conj |
on,on |
R1385 |
T4012 |
T4013 |
det |
the,expression |
R1386 |
T4013 |
T4011 |
pobj |
expression,on |
R1387 |
T4014 |
T4013 |
prep |
of,expression |
R1388 |
T4015 |
T4016 |
amod |
proprioceptive,afferent |
R1389 |
T4016 |
T4018 |
amod |
afferent,specific |
R1390 |
T4017 |
T4016 |
punct |
-,afferent |
R1391 |
T4018 |
T4020 |
amod |
specific,genes |
R1392 |
T4019 |
T4018 |
punct |
-,specific |
R1393 |
T4020 |
T4014 |
pobj |
genes,of |
R1394 |
T4021 |
T3978 |
punct |
.,assessed |
R1395 |
T4023 |
T4024 |
nmod |
Er81EWS,Pea3 |
R1396 |
T4024 |
T4026 |
nmod |
Pea3,mice |
R1397 |
T4025 |
T4024 |
punct |
-,Pea3 |
R1398 |
T4026 |
T4029 |
nsubj |
mice,differ |
R1399 |
T4027 |
T4024 |
punct |
/,Pea3 |
R1400 |
T4028 |
T4024 |
punct |
−,Pea3 |
R1401 |
T4030 |
T4029 |
aux |
did,differ |
R1402 |
T4031 |
T4029 |
neg |
not,differ |
R1403 |
T4032 |
T4029 |
prep |
from,differ |
R1404 |
T4033 |
T4034 |
amod |
wild,type |
R1405 |
T4034 |
T4032 |
pobj |
type,from |
R1406 |
T4035 |
T4034 |
punct |
-,type |
R1407 |
T4036 |
T4029 |
prep |
in,differ |
R1408 |
T4037 |
T4038 |
det |
the,number |
R1409 |
T4038 |
T4036 |
pobj |
number,in |
R1410 |
T4039 |
T4038 |
prep |
of,number |
R1411 |
T4040 |
T4041 |
amod |
proprioceptive,bodies |
R1412 |
T4041 |
T4039 |
pobj |
bodies,of |
R1413 |
T4042 |
T4041 |
amod |
afferent,bodies |
R1414 |
T4043 |
T4041 |
compound |
cell,bodies |
R1415 |
T4044 |
T4041 |
prep |
within,bodies |
R1416 |
T4045 |
T4046 |
det |
the,DRG |
R1417 |
T4046 |
T4044 |
pobj |
DRG,within |
R1418 |
T4047 |
T4046 |
prep |
of,DRG |
R1419 |
T4048 |
T4047 |
pobj |
L1,of |
R1420 |
T4049 |
T4048 |
prep |
to,L1 |
R1421 |
T4050 |
T4049 |
pobj |
L5,to |
R1422 |
T4051 |
T4038 |
punct |
", ",number |
R1423 |
T4052 |
T4053 |
det |
the,expression |
R1424 |
T4053 |
T4038 |
conj |
expression,number |
R1425 |
T4054 |
T4053 |
prep |
of,expression |
R1426 |
T4055 |
T4056 |
amod |
several,genes |
R1427 |
T4056 |
T4054 |
pobj |
genes,of |
R1428 |
T4057 |
T4058 |
advmod |
normally,expressed |
R1429 |
T4058 |
T4053 |
acl |
expressed,expression |
R1430 |
T4059 |
T4058 |
agent |
by,expressed |
R1431 |
T4060 |
T4061 |
amod |
proprioceptive,afferents |
R1432 |
T4061 |
T4059 |
pobj |
afferents,by |
R1433 |
T4062 |
T4053 |
punct |
", ",expression |
R1434 |
T4063 |
T4053 |
cc |
and,expression |
R1435 |
T4064 |
T4065 |
det |
the,lack |
R1436 |
T4065 |
T4053 |
conj |
lack,expression |
R1437 |
T4066 |
T4065 |
prep |
of,lack |
R1438 |
T4067 |
T4066 |
pobj |
expression,of |
R1439 |
T4068 |
T4067 |
prep |
of,expression |
R1440 |
T4069 |
T4068 |
pobj |
genes,of |
R1441 |
T4070 |
T4071 |
neg |
not,expressed |
R1442 |
T4071 |
T4069 |
acl |
expressed,genes |
R1443 |
T4072 |
T4071 |
advmod |
normally,expressed |
R1444 |
T4073 |
T4071 |
prep |
in,expressed |
R1445 |
T4074 |
T4075 |
amod |
proprioceptive,afferents |
R1446 |
T4075 |
T4073 |
pobj |
afferents,in |
R1447 |
T4076 |
T4077 |
punct |
(,S1 |
R1448 |
T4077 |
T4029 |
parataxis |
S1,differ |
R1449 |
T4078 |
T4077 |
compound |
Figure,S1 |
R1450 |
T4079 |
T4077 |
punct |
;,S1 |
R1451 |
T4080 |
T4081 |
nsubj |
data,shown |
R1452 |
T4081 |
T4077 |
ccomp |
shown,S1 |
R1453 |
T4082 |
T4081 |
neg |
not,shown |
R1454 |
T4083 |
T4077 |
punct |
),S1 |
R1455 |
T4084 |
T4029 |
punct |
.,differ |
R1456 |
T4086 |
T4087 |
advmod |
Together,suggest |
R1457 |
T4088 |
T4087 |
punct |
", ",suggest |
R1458 |
T4089 |
T4090 |
det |
these,findings |
R1459 |
T4090 |
T4087 |
nsubj |
findings,suggest |
R1460 |
T4091 |
T4092 |
mark |
that,mimics |
R1461 |
T4092 |
T4087 |
ccomp |
mimics,suggest |
R1462 |
T4093 |
T4094 |
det |
the,expression |
R1463 |
T4094 |
T4092 |
nsubj |
expression,mimics |
R1464 |
T4095 |
T4094 |
prep |
of,expression |
R1465 |
T4096 |
T4097 |
compound |
EWS,Pea3 |
R1466 |
T4097 |
T4095 |
pobj |
Pea3,of |
R1467 |
T4098 |
T4097 |
punct |
-,Pea3 |
R1468 |
T4099 |
T4094 |
prep |
from,expression |
R1469 |
T4100 |
T4101 |
det |
the,time |
R1470 |
T4101 |
T4099 |
pobj |
time,from |
R1471 |
T4102 |
T4101 |
amod |
normal,time |
R1472 |
T4103 |
T4101 |
prep |
of,time |
R1473 |
T4104 |
T4103 |
pobj |
onset,of |
R1474 |
T4105 |
T4106 |
det |
the,function |
R1475 |
T4106 |
T4092 |
dobj |
function,mimics |
R1476 |
T4107 |
T4106 |
prep |
of,function |
R1477 |
T4108 |
T4107 |
pobj |
Er81,of |
R1478 |
T4109 |
T4110 |
mark |
as,assessed |
R1479 |
T4110 |
T4092 |
advcl |
assessed,mimics |
R1480 |
T4111 |
T4110 |
prep |
by,assessed |
R1481 |
T4112 |
T4111 |
pobj |
induction,by |
R1482 |
T4113 |
T4112 |
cc |
and,induction |
R1483 |
T4114 |
T4112 |
conj |
maintenance,induction |
R1484 |
T4115 |
T4112 |
prep |
of,induction |
R1485 |
T4116 |
T4117 |
compound |
gene,expression |
R1486 |
T4117 |
T4115 |
pobj |
expression,of |
R1487 |
T4118 |
T4112 |
prep |
within,induction |
R1488 |
T4119 |
T4120 |
amod |
proprioceptive,afferents |
R1489 |
T4120 |
T4118 |
pobj |
afferents,within |
R1490 |
T4121 |
T4087 |
punct |
.,suggest |
R1491 |
T4123 |
T4124 |
aux |
To,determine |
R1492 |
T4124 |
T4125 |
advcl |
determine,traced |
R1493 |
T4126 |
T4127 |
det |
the,extent |
R1494 |
T4127 |
T4124 |
dobj |
extent,determine |
R1495 |
T4128 |
T4127 |
prep |
of,extent |
R1496 |
T4129 |
T4128 |
pobj |
rescue,of |
R1497 |
T4130 |
T4129 |
prep |
of,rescue |
R1498 |
T4131 |
T4132 |
nmod |
Ia,projections |
R1499 |
T4132 |
T4130 |
pobj |
projections,of |
R1500 |
T4133 |
T4132 |
amod |
proprioceptive,projections |
R1501 |
T4134 |
T4132 |
amod |
afferent,projections |
R1502 |
T4135 |
T4132 |
prep |
into,projections |
R1503 |
T4136 |
T4137 |
det |
the,cord |
R1504 |
T4137 |
T4135 |
pobj |
cord,into |
R1505 |
T4138 |
T4137 |
amod |
ventral,cord |
R1506 |
T4139 |
T4137 |
amod |
spinal,cord |
R1507 |
T4140 |
T4137 |
prep |
of,cord |
R1508 |
T4141 |
T4142 |
compound |
Er81,mice |
R1509 |
T4142 |
T4140 |
pobj |
mice,of |
R1510 |
T4143 |
T4142 |
compound |
mutant,mice |
R1511 |
T4144 |
T4132 |
acl |
achieved,projections |
R1512 |
T4145 |
T4144 |
agent |
by,achieved |
R1513 |
T4146 |
T4145 |
pobj |
expression,by |
R1514 |
T4147 |
T4146 |
prep |
of,expression |
R1515 |
T4148 |
T4149 |
compound |
EWS,Pea3 |
R1516 |
T4149 |
T4147 |
pobj |
Pea3,of |
R1517 |
T4150 |
T4149 |
punct |
-,Pea3 |
R1518 |
T4151 |
T4125 |
punct |
", ",traced |
R1519 |
T4152 |
T4125 |
nsubj |
we,traced |
R1520 |
T4153 |
T4154 |
amod |
intraspinal,projections |
R1521 |
T4154 |
T4125 |
dobj |
projections,traced |
R1522 |
T4155 |
T4154 |
amod |
afferent,projections |
R1523 |
T4156 |
T4125 |
prep |
by,traced |
R1524 |
T4157 |
T4158 |
amod |
axonal,labeling |
R1525 |
T4158 |
T4156 |
pobj |
labeling,by |
R1526 |
T4265 |
T4266 |
nmod |
vGlut1,terminals |
R1527 |
T4266 |
T4246 |
dobj |
terminals,formed |
R1528 |
T4267 |
T4265 |
punct |
+,vGlut1 |
R1529 |
T4268 |
T4269 |
dep |
that,were |
R1530 |
T4159 |
T4158 |
prep |
of,labeling |
R1531 |
T4269 |
T4266 |
relcl |
were,terminals |
R1532 |
T4160 |
T4159 |
pobj |
PV,of |
R1533 |
T4270 |
T4269 |
acomp |
absent,were |
R1534 |
T4161 |
T4162 |
punct |
(,2A |
R1535 |
T4162 |
T4125 |
parataxis |
2A,traced |
R1536 |
T4271 |
T4269 |
prep |
in,were |
R1537 |
T4163 |
T4162 |
compound |
Figure,2A |
R1538 |
T4164 |
T4165 |
punct |
–,2C |
R1539 |
T4165 |
T4162 |
prep |
2C,2A |
R1540 |
T4272 |
T4273 |
compound |
Er81,mice |
R1541 |
T4166 |
T4162 |
punct |
),2A |
R1542 |
T4167 |
T4125 |
punct |
.,traced |
R1543 |
T4273 |
T4271 |
pobj |
mice,in |
R1544 |
T4169 |
T4170 |
prep |
In,used |
R1545 |
T4274 |
T4273 |
compound |
mutant,mice |
R1546 |
T4171 |
T4169 |
pobj |
addition,In |
R1547 |
T4172 |
T4170 |
punct |
", ",used |
R1548 |
T4275 |
T4276 |
punct |
(,2G |
R1549 |
T4173 |
T4174 |
aux |
to,analyze |
R1550 |
T4174 |
T4170 |
advcl |
analyze,used |
R1551 |
T4276 |
T4269 |
parataxis |
2G,were |
R1552 |
T4175 |
T4176 |
compound |
axon,ingrowth |
R1553 |
T4176 |
T4174 |
dobj |
ingrowth,analyze |
R1554 |
T4177 |
T4176 |
amod |
independent,ingrowth |
R1555 |
T4277 |
T4276 |
compound |
Figure,2G |
R1556 |
T4178 |
T4177 |
prep |
of,independent |
R1557 |
T4179 |
T4180 |
det |
the,level |
R1558 |
T4180 |
T4178 |
pobj |
level,of |
R1559 |
T4278 |
T4279 |
punct |
–,2I |
R1560 |
T4181 |
T4180 |
prep |
of,level |
R1561 |
T4182 |
T4183 |
compound |
PV,expression |
R1562 |
T4279 |
T4276 |
prep |
2I,2G |
R1563 |
T4183 |
T4181 |
pobj |
expression,of |
R1564 |
T4184 |
T4180 |
prep |
in,level |
R1565 |
T4185 |
T4186 |
compound |
DRG,neurons |
R1566 |
T4186 |
T4184 |
pobj |
neurons,in |
R1567 |
T4187 |
T4170 |
punct |
", ",used |
R1568 |
T4280 |
T4276 |
punct |
),2G |
R1569 |
T4188 |
T4170 |
nsubj |
we,used |
R1570 |
T4189 |
T4190 |
amod |
anterograde,labeling |
R1571 |
T4190 |
T4170 |
dobj |
labeling,used |
R1572 |
T4281 |
T4246 |
punct |
.,formed |
R1573 |
T4191 |
T4190 |
prep |
of,labeling |
R1574 |
T4192 |
T4193 |
amod |
afferent,fibers |
R1575 |
T4283 |
T4284 |
aux |
To,assess |
R1576 |
T4193 |
T4191 |
pobj |
fibers,of |
R1577 |
T4284 |
T4285 |
advcl |
assess,performed |
R1578 |
T4194 |
T4170 |
prep |
by,used |
R1579 |
T4286 |
T4287 |
mark |
whether,are |
R1580 |
T4195 |
T4194 |
pcomp |
applying,by |
R1581 |
T4196 |
T4197 |
advmod |
fluorescently,labeled |
R1582 |
T4197 |
T4198 |
amod |
labeled,dextran |
R1583 |
T4198 |
T4195 |
dobj |
dextran,applying |
R1584 |
T4199 |
T4195 |
prep |
to,applying |
R1585 |
T4287 |
T4284 |
ccomp |
are,assess |
R1586 |
T4200 |
T4201 |
amod |
cut,roots |
R1587 |
T4201 |
T4199 |
pobj |
roots,to |
R1588 |
T4202 |
T4201 |
amod |
dorsal,roots |
R1589 |
T4288 |
T4287 |
nsubj |
synapses,are |
R1590 |
T4203 |
T4204 |
punct |
(,2D |
R1591 |
T4204 |
T4195 |
parataxis |
2D,applying |
R1592 |
T4205 |
T4204 |
compound |
Figure,2D |
R1593 |
T4289 |
T4288 |
prep |
between,synapses |
R1594 |
T4206 |
T4207 |
punct |
–,2F |
R1595 |
T4207 |
T4204 |
prep |
2F,2D |
R1596 |
T4208 |
T4204 |
punct |
),2D |
R1597 |
T4290 |
T4291 |
compound |
Ia,afferents |
R1598 |
T4209 |
T4170 |
punct |
.,used |
R1599 |
T4291 |
T4289 |
pobj |
afferents,between |
R1600 |
T4211 |
T4212 |
advcl |
Using,found |
R1601 |
T4213 |
T4214 |
det |
both,assays |
R1602 |
T4292 |
T4291 |
cc |
and,afferents |
R1603 |
T4214 |
T4211 |
dobj |
assays,Using |
R1604 |
T4293 |
T4294 |
compound |
motor,neurons |
R1605 |
T4215 |
T4212 |
punct |
", ",found |
R1606 |
T4294 |
T4291 |
conj |
neurons,afferents |
R1607 |
T4216 |
T4212 |
nsubj |
we,found |
R1608 |
T4217 |
T4218 |
amod |
extensive,rescue |
R1609 |
T4218 |
T4212 |
dobj |
rescue,found |
R1610 |
T4295 |
T4287 |
acomp |
functional,are |
R1611 |
T4219 |
T4218 |
prep |
of,rescue |
R1612 |
T4220 |
T4221 |
det |
the,projections |
R1613 |
T4296 |
T4287 |
prep |
in,are |
R1614 |
T4221 |
T4219 |
pobj |
projections,of |
R1615 |
T4222 |
T4221 |
prep |
into,projections |
R1616 |
T4223 |
T4224 |
det |
the,horn |
R1617 |
T4224 |
T4222 |
pobj |
horn,into |
R1618 |
T4225 |
T4224 |
amod |
ventral,horn |
R1619 |
T4297 |
T4298 |
nmod |
Er81EWS,Pea3 |
R1620 |
T4226 |
T4224 |
prep |
of,horn |
R1621 |
T4227 |
T4228 |
det |
the,cord |
R1622 |
T4228 |
T4226 |
pobj |
cord,of |
R1623 |
T4298 |
T4300 |
nmod |
Pea3,mice |
R1624 |
T4229 |
T4228 |
amod |
spinal,cord |
R1625 |
T4230 |
T4212 |
prep |
in,found |
R1626 |
T4299 |
T4298 |
punct |
-,Pea3 |
R1627 |
T4231 |
T4232 |
nmod |
Er81EWS,Pea3 |
R1628 |
T4232 |
T4234 |
nmod |
Pea3,mice |
R1629 |
T4233 |
T4232 |
punct |
-,Pea3 |
R1630 |
T4300 |
T4296 |
pobj |
mice,in |
R1631 |
T4234 |
T4230 |
pobj |
mice,in |
R1632 |
T4235 |
T4232 |
punct |
/,Pea3 |
R1633 |
T4236 |
T4232 |
punct |
−,Pea3 |
R1634 |
T4301 |
T4298 |
punct |
/,Pea3 |
R1635 |
T4237 |
T4238 |
punct |
(,2C |
R1636 |
T4238 |
T4212 |
parataxis |
2C,found |
R1637 |
T4239 |
T4238 |
compound |
Figure,2C |
R1638 |
T4302 |
T4298 |
punct |
−,Pea3 |
R1639 |
T4240 |
T4238 |
cc |
and,2C |
R1640 |
T4241 |
T4238 |
conj |
2F,2C |
R1641 |
T4242 |
T4238 |
punct |
),2C |
R1642 |
T4303 |
T4285 |
punct |
", ",performed |
R1643 |
T4243 |
T4212 |
punct |
.,found |
R1644 |
T4304 |
T4285 |
nsubj |
we,performed |
R1645 |
T4245 |
T4246 |
prep |
Within,formed |
R1646 |
T4247 |
T4248 |
det |
the,horn |
R1647 |
T4305 |
T4306 |
amod |
intracellular,recordings |
R1648 |
T4248 |
T4245 |
pobj |
horn,Within |
R1649 |
T4249 |
T4248 |
amod |
ventral,horn |
R1650 |
T4250 |
T4246 |
punct |
", ",formed |
R1651 |
T4306 |
T4285 |
dobj |
recordings,performed |
R1652 |
T4251 |
T4252 |
compound |
Ia,afferents |
R1653 |
T4252 |
T4246 |
nsubj |
afferents,formed |
R1654 |
T4253 |
T4252 |
prep |
in,afferents |
R1655 |
T4307 |
T4306 |
prep |
from,recordings |
R1656 |
T4254 |
T4255 |
preconj |
both,type |
R1657 |
T4255 |
T4258 |
nmod |
type,mice |
R1658 |
T4256 |
T4255 |
amod |
wild,type |
R1659 |
T4257 |
T4255 |
punct |
-,type |
R1660 |
T4308 |
T4309 |
amod |
identified,neurons |
R1661 |
T4258 |
T4253 |
pobj |
mice,in |
R1662 |
T4259 |
T4255 |
cc |
and,type |
R1663 |
T4260 |
T4261 |
compound |
Er81EWS,Pea3 |
R1664 |
T4309 |
T4307 |
pobj |
neurons,from |
R1665 |
T4261 |
T4255 |
conj |
Pea3,type |
R1666 |
T4262 |
T4261 |
punct |
-,Pea3 |
R1667 |
T4263 |
T4261 |
punct |
/,Pea3 |
R1668 |
T4264 |
T4261 |
punct |
−,Pea3 |
R1669 |
T4310 |
T4309 |
compound |
quadriceps,neurons |
R1670 |
T4311 |
T4309 |
compound |
motor,neurons |
R1671 |
T4312 |
T4285 |
prep |
after,performed |
R1672 |
T4313 |
T4312 |
pobj |
stimulation,after |
R1673 |
T4314 |
T4313 |
prep |
of,stimulation |
R1674 |
T4371 |
T4368 |
punct |
", ",8 |
R1675 |
T4315 |
T4314 |
pobj |
nerves,of |
R1676 |
T4372 |
T4373 |
quantmod |
10.9,1 |
R1677 |
T4373 |
T4375 |
nummod |
1,mV |
R1678 |
T4316 |
T4315 |
acl |
innervating,nerves |
R1679 |
T4374 |
T4373 |
punct |
±,1 |
R1680 |
T4375 |
T4368 |
dep |
mV,8 |
R1681 |
T4376 |
T4368 |
punct |
", ",8 |
R1682 |
T4317 |
T4318 |
det |
the,group |
R1683 |
T4377 |
T4368 |
nsubj |
n,8 |
R1684 |
T4378 |
T4368 |
punct |
=,8 |
R1685 |
T4379 |
T4349 |
punct |
),S2 |
R1686 |
T4318 |
T4316 |
dobj |
group,innervating |
R1687 |
T4380 |
T4324 |
punct |
.,found |
R1688 |
T4382 |
T4383 |
advmod |
Together,suggest |
R1689 |
T4319 |
T4318 |
compound |
quadriceps,group |
R1690 |
T4384 |
T4383 |
punct |
", ",suggest |
R1691 |
T4320 |
T4318 |
compound |
muscle,group |
R1692 |
T4321 |
T4285 |
punct |
.,performed |
R1693 |
T4385 |
T4386 |
det |
these,findings |
R1694 |
T4386 |
T4383 |
nsubj |
findings,suggest |
R1695 |
T4323 |
T4324 |
nsubj |
We,found |
R1696 |
T4387 |
T4388 |
mark |
that,direct |
R1697 |
T4388 |
T4383 |
ccomp |
direct,suggest |
R1698 |
T4389 |
T4388 |
prep |
in,direct |
R1699 |
T4325 |
T4326 |
det |
no,difference |
R1700 |
T4390 |
T4391 |
det |
the,absence |
R1701 |
T4391 |
T4389 |
pobj |
absence,in |
R1702 |
T4392 |
T4391 |
prep |
of,absence |
R1703 |
T4393 |
T4392 |
pobj |
Er81,of |
R1704 |
T4326 |
T4324 |
dobj |
difference,found |
R1705 |
T4327 |
T4326 |
amod |
significant,difference |
R1706 |
T4328 |
T4326 |
prep |
in,difference |
R1707 |
T4329 |
T4330 |
det |
the,amplitude |
R1708 |
T4394 |
T4388 |
punct |
", ",direct |
R1709 |
T4330 |
T4328 |
pobj |
amplitude,in |
R1710 |
T4395 |
T4396 |
compound |
EWS,Pea3 |
R1711 |
T4396 |
T4388 |
nsubj |
Pea3,direct |
R1712 |
T4331 |
T4330 |
compound |
input,amplitude |
R1713 |
T4397 |
T4396 |
punct |
-,Pea3 |
R1714 |
T4398 |
T4388 |
aux |
can,direct |
R1715 |
T4332 |
T4330 |
prep |
to,amplitude |
R1716 |
T4399 |
T4400 |
det |
the,process |
R1717 |
T4400 |
T4388 |
dobj |
process,direct |
R1718 |
T4401 |
T4400 |
amod |
complex,process |
R1719 |
T4333 |
T4334 |
compound |
quadriceps,neurons |
R1720 |
T4402 |
T4400 |
amod |
biological,process |
R1721 |
T4403 |
T4400 |
prep |
of,process |
R1722 |
T4404 |
T4405 |
amod |
correct,termination |
R1723 |
T4405 |
T4403 |
pobj |
termination,of |
R1724 |
T4406 |
T4405 |
amod |
laminar,termination |
R1725 |
T4407 |
T4405 |
prep |
within,termination |
R1726 |
T4334 |
T4332 |
pobj |
neurons,to |
R1727 |
T4408 |
T4409 |
det |
the,cord |
R1728 |
T4409 |
T4407 |
pobj |
cord,within |
R1729 |
T4410 |
T4409 |
amod |
ventral,cord |
R1730 |
T4335 |
T4334 |
compound |
motor,neurons |
R1731 |
T4411 |
T4409 |
amod |
spinal,cord |
R1732 |
T4412 |
T4400 |
cc |
and,process |
R1733 |
T4413 |
T4414 |
det |
the,formation |
R1734 |
T4336 |
T4337 |
advmod |
when,comparing |
R1735 |
T4414 |
T4400 |
conj |
formation,process |
R1736 |
T4415 |
T4414 |
prep |
of,formation |
R1737 |
T4416 |
T4415 |
pobj |
synapses,of |
R1738 |
T4337 |
T4324 |
advcl |
comparing,found |
R1739 |
T4417 |
T4416 |
prep |
with,synapses |
R1740 |
T4418 |
T4419 |
compound |
motor,neurons |
R1741 |
T4338 |
T4339 |
amod |
wild,type |
R1742 |
T4419 |
T4417 |
pobj |
neurons,with |
R1743 |
T4420 |
T4421 |
punct |
(,2J |
R1744 |
T4339 |
T4337 |
dobj |
type,comparing |
R1745 |
T4421 |
T4388 |
parataxis |
2J,direct |
R1746 |
T4422 |
T4421 |
compound |
Figure,2J |
R1747 |
T4423 |
T4424 |
punct |
–,2L |
R1748 |
T4340 |
T4339 |
punct |
-,type |
R1749 |
T4424 |
T4421 |
prep |
2L,2J |
R1750 |
T4425 |
T4421 |
punct |
),2J |
R1751 |
T4426 |
T4388 |
punct |
", ",direct |
R1752 |
T4341 |
T4337 |
prep |
to,comparing |
R1753 |
T4427 |
T4428 |
advmod |
thus,identifying |
R1754 |
T4428 |
T4388 |
advcl |
identifying,direct |
R1755 |
T4429 |
T4430 |
det |
an,factor |
R1756 |
T4342 |
T4343 |
compound |
Er81EWS,Pea3 |
R1757 |
T4430 |
T4428 |
dobj |
factor,identifying |
R1758 |
T4431 |
T4430 |
compound |
ETS,factor |
R1759 |
T4343 |
T4341 |
pobj |
Pea3,to |
R1760 |
T4432 |
T4430 |
compound |
transcription,factor |
R1761 |
T4433 |
T4430 |
amod |
suitable,factor |
R1762 |
T4434 |
T4433 |
prep |
for,suitable |
R1763 |
T4344 |
T4343 |
punct |
-,Pea3 |
R1764 |
T4435 |
T4436 |
amod |
heterochronic,experiments |
R1765 |
T4436 |
T4434 |
pobj |
experiments,for |
R1766 |
T4437 |
T4436 |
compound |
expression,experiments |
R1767 |
T4345 |
T4343 |
punct |
/,Pea3 |
R1768 |
T4438 |
T4436 |
prep |
in,experiments |
R1769 |
T4439 |
T4440 |
compound |
DRG,neurons |
R1770 |
T4440 |
T4438 |
pobj |
neurons,in |
R1771 |
T4346 |
T4343 |
punct |
−,Pea3 |
R1772 |
T4441 |
T4383 |
punct |
.,suggest |
R1773 |
T4347 |
T4341 |
pobj |
mice,to |
R1774 |
T4348 |
T4349 |
punct |
(,S2 |
R1775 |
T4349 |
T4337 |
parataxis |
S2,comparing |
R1776 |
T4350 |
T4349 |
compound |
Figure,S2 |
R1777 |
T4351 |
T4349 |
punct |
;,S2 |
R1778 |
T4352 |
T4353 |
amod |
wild,type |
R1779 |
T4353 |
T4355 |
dep |
type,11 |
R1780 |
T4354 |
T4353 |
punct |
-,type |
R1781 |
T4355 |
T4349 |
dep |
11,S2 |
R1782 |
T4356 |
T4355 |
punct |
", ",11 |
R1783 |
T4357 |
T4358 |
quantmod |
10.6,0.9 |
R1784 |
T4358 |
T4360 |
nummod |
0.9,mV |
R1785 |
T4359 |
T4358 |
punct |
±,0.9 |
R1786 |
T4360 |
T4355 |
dep |
mV,11 |
R1787 |
T4361 |
T4355 |
punct |
", ",11 |
R1788 |
T4362 |
T4355 |
nsubj |
n,11 |
R1789 |
T4363 |
T4355 |
punct |
=,11 |
R1790 |
T4364 |
T4349 |
punct |
;,S2 |
R1791 |
T4365 |
T4366 |
compound |
Er81EWS,Pea3 |
R1792 |
T4366 |
T4368 |
dep |
Pea3,8 |
R1793 |
T4367 |
T4366 |
punct |
-,Pea3 |
R1794 |
T4368 |
T4349 |
dep |
8,S2 |
R1795 |
T4369 |
T4366 |
punct |
/,Pea3 |
R1796 |
T4370 |
T4366 |
punct |
−,Pea3 |
R1797 |
T5254 |
T5255 |
amod |
Precocious,Expression |
R1798 |
T5255 |
T5256 |
nsubj |
Expression,Leads |
R1799 |
T5257 |
T5255 |
prep |
of,Expression |
R1800 |
T5258 |
T5259 |
compound |
EWS,Pea3 |
R1801 |
T5259 |
T5257 |
pobj |
Pea3,of |
R1802 |
T5260 |
T5259 |
punct |
-,Pea3 |
R1803 |
T5261 |
T5255 |
prep |
in,Expression |
R1804 |
T5262 |
T5263 |
compound |
DRG,Neurons |
R1805 |
T5263 |
T5261 |
pobj |
Neurons,in |
R1806 |
T5264 |
T5256 |
prep |
to,Leads |
R1807 |
T5265 |
T5266 |
amod |
Axonal,Defects |
R1808 |
T5266 |
T5264 |
pobj |
Defects,to |
R1809 |
T5267 |
T5266 |
compound |
Projection,Defects |
R1810 |
T5269 |
T5270 |
aux |
To,address |
R1811 |
T5270 |
T5271 |
advcl |
address,expressed |
R1812 |
T5272 |
T5273 |
det |
the,consequences |
R1813 |
T5273 |
T5270 |
dobj |
consequences,address |
R1814 |
T5274 |
T5273 |
prep |
of,consequences |
R1815 |
T5275 |
T5276 |
amod |
precocious,signaling |
R1816 |
T5276 |
T5274 |
pobj |
signaling,of |
R1817 |
T5277 |
T5276 |
compound |
ETS,signaling |
R1818 |
T5278 |
T5273 |
prep |
for,consequences |
R1819 |
T5279 |
T5280 |
amod |
proprioceptive,afferent |
R1820 |
T5280 |
T5281 |
nmod |
afferent,differentiation |
R1821 |
T5281 |
T5278 |
pobj |
differentiation,for |
R1822 |
T5282 |
T5271 |
punct |
", ",expressed |
R1823 |
T5283 |
T5271 |
nsubj |
we,expressed |
R1824 |
T5284 |
T5271 |
advmod |
next,expressed |
R1825 |
T5285 |
T5286 |
compound |
EWS,Pea3 |
R1826 |
T5286 |
T5271 |
dobj |
Pea3,expressed |
R1827 |
T5287 |
T5286 |
punct |
-,Pea3 |
R1828 |
T5288 |
T5271 |
prep |
in,expressed |
R1829 |
T5289 |
T5290 |
compound |
DRG,neurons |
R1830 |
T5290 |
T5288 |
pobj |
neurons,in |
R1831 |
T5291 |
T5292 |
advmod |
as,soon |
R1832 |
T5292 |
T5271 |
advmod |
soon,expressed |
R1833 |
T5293 |
T5294 |
mark |
as,became |
R1834 |
T5294 |
T5292 |
advcl |
became,soon |
R1835 |
T5295 |
T5294 |
nsubj |
they,became |
R1836 |
T5296 |
T5294 |
acomp |
post-mitotic,became |
R1837 |
T5297 |
T5271 |
punct |
.,expressed |
R1838 |
T5299 |
T5300 |
nsubj |
We,used |
R1839 |
T5301 |
T5302 |
det |
a,system |
R1840 |
T5302 |
T5300 |
dobj |
system,used |
R1841 |
T5303 |
T5302 |
amod |
binary,system |
R1842 |
T5304 |
T5302 |
nmod |
mouse,system |
R1843 |
T5305 |
T5302 |
amod |
genetic,system |
R1844 |
T5306 |
T5302 |
prep |
based,system |
R1845 |
T5307 |
T5306 |
prep |
on,based |
R1846 |
T5308 |
T5309 |
compound |
Cre,recombinase |
R1847 |
T5309 |
T5311 |
npadvmod |
recombinase,mediated |
R1848 |
T5310 |
T5309 |
punct |
-,recombinase |
R1849 |
T5311 |
T5313 |
amod |
mediated,excision |
R1850 |
T5312 |
T5311 |
punct |
-,mediated |
R1851 |
T5313 |
T5307 |
pobj |
excision,on |
R1852 |
T5314 |
T5313 |
prep |
of,excision |
R1853 |
T5315 |
T5316 |
det |
a,cassette |
R1854 |
T5316 |
T5314 |
pobj |
cassette,of |
R1855 |
T5317 |
T5316 |
amod |
transcriptional,cassette |
R1856 |
T5318 |
T5316 |
compound |
stop,cassette |
R1857 |
T5319 |
T5316 |
acl |
flanked,cassette |
R1858 |
T5320 |
T5319 |
agent |
by,flanked |
R1859 |
T5321 |
T5322 |
compound |
loxP,sites |
R1860 |
T5322 |
T5320 |
pobj |
sites,by |
R1861 |
T5323 |
T5300 |
punct |
.,used |
R1862 |
T5325 |
T5326 |
compound |
Targeting,cassettes |
R1863 |
T5326 |
T5327 |
nsubjpass |
cassettes,integrated |
R1864 |
T5328 |
T5327 |
auxpass |
were,integrated |
R1865 |
T5329 |
T5327 |
prep |
into,integrated |
R1866 |
T5330 |
T5331 |
det |
the,locus |
R1867 |
T5331 |
T5329 |
pobj |
locus,into |
R1868 |
T5332 |
T5331 |
compound |
Tau,locus |
R1869 |
T5333 |
T5334 |
aux |
to,generate |
R1870 |
T5334 |
T5327 |
advcl |
generate,integrated |
R1871 |
T5335 |
T5336 |
nummod |
two,strains |
R1872 |
T5336 |
T5334 |
dobj |
strains,generate |
R1873 |
T5337 |
T5336 |
prep |
of,strains |
R1874 |
T5338 |
T5337 |
pobj |
mice,of |
R1875 |
T5339 |
T5340 |
advmod |
conditionally,expressing |
R1876 |
T5340 |
T5336 |
acl |
expressing,strains |
R1877 |
T5341 |
T5342 |
preconj |
either,Pea3 |
R1878 |
T5342 |
T5340 |
dobj |
Pea3,expressing |
R1879 |
T5343 |
T5342 |
compound |
EWS,Pea3 |
R1880 |
T5344 |
T5342 |
punct |
-,Pea3 |
R1881 |
T5345 |
T5342 |
cc |
or,Pea3 |
R1882 |
T5346 |
T5347 |
det |
a,protein |
R1883 |
T5347 |
T5342 |
conj |
protein,Pea3 |
R1884 |
T5348 |
T5349 |
npadvmod |
membrane,targeted |
R1885 |
T5349 |
T5347 |
amod |
targeted,protein |
R1886 |
T5350 |
T5349 |
punct |
-,targeted |
R1887 |
T5351 |
T5347 |
amod |
green,protein |
R1888 |
T5352 |
T5347 |
amod |
fluorescent,protein |
R1889 |
T5353 |
T5347 |
punct |
(,protein |
R1890 |
T5354 |
T5347 |
appos |
mGFP,protein |
R1891 |
T5355 |
T5347 |
punct |
),protein |
R1892 |
T5356 |
T5357 |
aux |
to,trace |
R1893 |
T5357 |
T5340 |
advcl |
trace,expressing |
R1894 |
T5358 |
T5359 |
amod |
axonal,projections |
R1895 |
T5359 |
T5357 |
dobj |
projections,trace |
R1896 |
T5360 |
T5359 |
prep |
of,projections |
R1897 |
T5361 |
T5362 |
compound |
DRG,neurons |
R1898 |
T5362 |
T5360 |
pobj |
neurons,of |
R1899 |
T5363 |
T5364 |
punct |
(,25 |
R1900 |
T5364 |
T5334 |
parataxis |
25,generate |
R1901 |
T5365 |
T5366 |
compound |
Figure,S3 |
R1902 |
T5366 |
T5364 |
dep |
S3,25 |
R1903 |
T5367 |
T5364 |
punct |
;,25 |
R1904 |
T5368 |
T5364 |
punct |
[,25 |
R1905 |
T5369 |
T5364 |
punct |
],25 |
R1906 |
T5370 |
T5364 |
punct |
),25 |
R1907 |
T5371 |
T5327 |
punct |
.,integrated |
R1908 |
T5373 |
T5374 |
nsubj |
Embryos,showed |
R1909 |
T5375 |
T5373 |
amod |
positive,Embryos |
R1910 |
T5376 |
T5375 |
prep |
for,positive |
R1911 |
T5377 |
T5378 |
preconj |
either,Isl1Cre |
R1912 |
T5378 |
T5379 |
nmod |
Isl1Cre,alleles |
R1913 |
T5379 |
T5376 |
pobj |
alleles,for |
R1914 |
T5380 |
T5378 |
punct |
/,Isl1Cre |
R1915 |
T5381 |
T5378 |
punct |
+,Isl1Cre |
R1916 |
T5382 |
T5378 |
cc |
and,Isl1Cre |
R1917 |
T5383 |
T5384 |
compound |
TauEWS,Pea3 |
R1918 |
T5384 |
T5378 |
conj |
Pea3,Isl1Cre |
R1919 |
T5385 |
T5384 |
punct |
-,Pea3 |
R1920 |
T5386 |
T5384 |
punct |
/,Pea3 |
R1921 |
T5387 |
T5384 |
punct |
+,Pea3 |
R1922 |
T5388 |
T5378 |
cc |
or,Isl1Cre |
R1923 |
T5389 |
T5378 |
conj |
Isl1Cre,Isl1Cre |
R1924 |
T5390 |
T5389 |
punct |
/,Isl1Cre |
R1925 |
T5391 |
T5389 |
punct |
+,Isl1Cre |
R1926 |
T5392 |
T5389 |
cc |
and,Isl1Cre |
R1927 |
T5393 |
T5389 |
conj |
TaumGFP,Isl1Cre |
R1928 |
T5394 |
T5393 |
punct |
/,TaumGFP |
R1929 |
T5395 |
T5393 |
punct |
+,TaumGFP |
R1930 |
T5396 |
T5397 |
amod |
efficient,activation |
R1931 |
T5397 |
T5374 |
dobj |
activation,showed |
R1932 |
T5398 |
T5397 |
prep |
of,activation |
R1933 |
T5399 |
T5400 |
det |
the,allele |
R1934 |
T5400 |
T5398 |
pobj |
allele,of |
R1935 |
T5401 |
T5400 |
amod |
silent,allele |
R1936 |
T5402 |
T5400 |
compound |
Tau,allele |
R1937 |
T5403 |
T5374 |
prep |
in,showed |
R1938 |
T5404 |
T5405 |
nummod |
95,% |
R1939 |
T5405 |
T5403 |
pobj |
%,in |
R1940 |
T5406 |
T5407 |
cc |
or,more |
R1941 |
T5407 |
T5405 |
nummod |
more,% |
R1942 |
T5408 |
T5405 |
prep |
of,% |
R1943 |
T5409 |
T5410 |
det |
all,neurons |
R1944 |
T5410 |
T5408 |
pobj |
neurons,of |
R1945 |
T5411 |
T5410 |
compound |
DRG,neurons |
R1946 |
T5412 |
T5405 |
punct |
", ",% |
R1947 |
T5413 |
T5405 |
prep |
including,% |
R1948 |
T5414 |
T5415 |
amod |
proprioceptive,afferents |
R1949 |
T5415 |
T5413 |
pobj |
afferents,including |
R1950 |
T5416 |
T5374 |
punct |
", ",showed |
R1951 |
T5417 |
T5374 |
prep |
at,showed |
R1952 |
T5418 |
T5419 |
det |
all,levels |
R1953 |
T5419 |
T5417 |
pobj |
levels,at |
R1954 |
T5420 |
T5419 |
amod |
segmental,levels |
R1955 |
T5421 |
T5422 |
punct |
(,S3 |
R1956 |
T5422 |
T5374 |
parataxis |
S3,showed |
R1957 |
T5423 |
T5422 |
compound |
Figure,S3 |
R1958 |
T5424 |
T5422 |
punct |
),S3 |
R1959 |
T5425 |
T5374 |
punct |
.,showed |
R1960 |
T5427 |
T5428 |
nsubj |
We,assessed |
R1961 |
T5429 |
T5428 |
advmod |
first,assessed |
R1962 |
T5430 |
T5431 |
det |
the,influence |
R1963 |
T5431 |
T5428 |
dobj |
influence,assessed |
R1964 |
T5432 |
T5431 |
prep |
of,influence |
R1965 |
T5433 |
T5434 |
compound |
EWS,Pea3 |
R1966 |
T5434 |
T5436 |
compound |
Pea3,expression |
R1967 |
T5435 |
T5434 |
punct |
-,Pea3 |
R1968 |
T5436 |
T5432 |
pobj |
expression,of |
R1969 |
T5437 |
T5436 |
prep |
in,expression |
R1970 |
T5438 |
T5439 |
amod |
early,neurons |
R1971 |
T5439 |
T5437 |
pobj |
neurons,in |
R1972 |
T5440 |
T5439 |
amod |
post-mitotic,neurons |
R1973 |
T5441 |
T5439 |
compound |
DRG,neurons |
R1974 |
T5442 |
T5431 |
prep |
on,influence |
R1975 |
T5443 |
T5444 |
det |
the,establishment |
R1976 |
T5444 |
T5442 |
pobj |
establishment,on |
R1977 |
T5445 |
T5444 |
prep |
of,establishment |
R1978 |
T5446 |
T5447 |
amod |
afferent,projections |
R1979 |
T5447 |
T5445 |
pobj |
projections,of |
R1980 |
T5448 |
T5447 |
prep |
into,projections |
R1981 |
T5449 |
T5450 |
det |
the,cord |
R1982 |
T5450 |
T5448 |
pobj |
cord,into |
R1983 |
T5451 |
T5450 |
amod |
spinal,cord |
R1984 |
T5452 |
T5444 |
acl |
using,establishment |
R1985 |
T5453 |
T5454 |
det |
the,allele |
R1986 |
T5454 |
T5452 |
dobj |
allele,using |
R1987 |
T5455 |
T5454 |
nmod |
TaumGFP,allele |
R1988 |
T5456 |
T5454 |
punct |
/,allele |
R1989 |
T5457 |
T5454 |
punct |
+,allele |
R1990 |
T5458 |
T5454 |
cc |
or,allele |
R1991 |
T5459 |
T5460 |
det |
a,protein |
R1992 |
T5460 |
T5454 |
conj |
protein,allele |
R1993 |
T5461 |
T5462 |
compound |
Thy1,promoter |
R1994 |
T5462 |
T5464 |
npadvmod |
promoter,driven |
R1995 |
T5463 |
T5462 |
punct |
-,promoter |
R1996 |
T5464 |
T5460 |
amod |
driven,protein |
R1997 |
T5465 |
T5464 |
punct |
-,driven |
R1998 |
T5466 |
T5460 |
nmod |
synaptophysin,protein |
R1999 |
T5467 |
T5460 |
amod |
green,protein |
R2000 |
T5468 |
T5460 |
amod |
fluorescent,protein |
R2001 |
T5469 |
T5460 |
punct |
(,protein |
R2002 |
T5470 |
T5460 |
appos |
spGFP,protein |
R2003 |
T5471 |
T5428 |
punct |
),assessed |
R2004 |
T5472 |
T5473 |
mark |
with,restricted |
R2005 |
T5473 |
T5428 |
advcl |
restricted,assessed |
R2006 |
T5474 |
T5475 |
det |
an,profile |
R2007 |
T5475 |
T5473 |
nsubj |
profile,restricted |
R2008 |
T5476 |
T5475 |
compound |
expression,profile |
R2009 |
T5477 |
T5473 |
prep |
to,restricted |
R2010 |
T5478 |
T5479 |
nmod |
DRG,neurons |
R2011 |
T5479 |
T5477 |
pobj |
neurons,to |
R2012 |
T5480 |
T5479 |
amod |
sensory,neurons |
R2013 |
T5481 |
T5473 |
prep |
at,restricted |
R2014 |
T5482 |
T5483 |
amod |
embryonic,day |
R2015 |
T5483 |
T5481 |
pobj |
day,at |
R2016 |
T5484 |
T5483 |
punct |
(,day |
R2017 |
T5485 |
T5483 |
appos |
E,day |
R2018 |
T5486 |
T5483 |
punct |
),day |
R2019 |
T5487 |
T5483 |
nummod |
13.5,day |
R2020 |
T5488 |
T5489 |
punct |
(,Figure |
R2021 |
T5489 |
T5473 |
parataxis |
Figure,restricted |
R2022 |
T5490 |
T5489 |
dep |
Thy1spGFP,Figure |
R2023 |
T5491 |
T5489 |
punct |
;,Figure |
R2024 |
T5492 |
T5489 |
punct |
[,Figure |
R2025 |
T5493 |
T5489 |
dep |
25,Figure |
R2026 |
T5494 |
T5489 |
punct |
],Figure |
R2027 |
T5495 |
T5489 |
punct |
),Figure |
R2028 |
T5496 |
T5489 |
punct |
(,Figure |
R2029 |
T5497 |
T5489 |
nummod |
3,Figure |
R2030 |
T5498 |
T5489 |
punct |
),Figure |
R2031 |
T5499 |
T5428 |
punct |
.,assessed |
R2032 |
T5501 |
T5502 |
prep |
In,failed |
R2033 |
T5503 |
T5501 |
pobj |
contrast,In |
R2034 |
T5504 |
T5503 |
prep |
to,contrast |
R2035 |
T5505 |
T5506 |
amod |
wild,type |
R2036 |
T5506 |
T5508 |
nmod |
type,projections |
R2037 |
T5507 |
T5506 |
punct |
-,type |
R2038 |
T5508 |
T5504 |
pobj |
projections,to |
R2039 |
T5509 |
T5508 |
amod |
proprioceptive,projections |
R2040 |
T5510 |
T5508 |
amod |
afferent,projections |
R2041 |
T5511 |
T5512 |
punct |
(,3A |
R2042 |
T5512 |
T5508 |
parataxis |
3A,projections |
R2043 |
T5513 |
T5512 |
compound |
Figure,3A |
R2044 |
T5514 |
T5515 |
punct |
–,3C |
R2045 |
T5515 |
T5512 |
prep |
3C,3A |
R2046 |
T5516 |
T5512 |
punct |
),3A |
R2047 |
T5517 |
T5502 |
punct |
", ",failed |
R2048 |
T5518 |
T5519 |
nmod |
GFP,afferents |
R2049 |
T5519 |
T5502 |
nsubj |
afferents,failed |
R2050 |
T5520 |
T5518 |
punct |
+,GFP |
R2051 |
T5521 |
T5519 |
amod |
sensory,afferents |
R2052 |
T5522 |
T5519 |
prep |
in,afferents |
R2053 |
T5523 |
T5524 |
nmod |
TauEWS,Pea3 |
R2054 |
T5524 |
T5526 |
nmod |
Pea3,embryos |
R2055 |
T5525 |
T5524 |
punct |
-,Pea3 |
R2056 |
T5526 |
T5522 |
pobj |
embryos,in |
R2057 |
T5527 |
T5524 |
punct |
/,Pea3 |
R2058 |
T5528 |
T5524 |
punct |
+,Pea3 |
R2059 |
T5529 |
T5524 |
appos |
Isl1Cre,Pea3 |
R2060 |
T5530 |
T5529 |
punct |
/,Isl1Cre |
R2061 |
T5531 |
T5529 |
punct |
+,Isl1Cre |
R2062 |
T5532 |
T5533 |
aux |
to,invade |
R2063 |
T5533 |
T5502 |
xcomp |
invade,failed |
R2064 |
T5534 |
T5535 |
det |
the,cord |
R2065 |
T5535 |
T5533 |
dobj |
cord,invade |
R2066 |
T5536 |
T5535 |
amod |
spinal,cord |
R2067 |
T5537 |
T5502 |
cc |
and,failed |
R2068 |
T5538 |
T5539 |
advmod |
instead,found |
R2069 |
T5539 |
T5502 |
conj |
found,failed |
R2070 |
T5540 |
T5539 |
auxpass |
were,found |
R2071 |
T5541 |
T5539 |
oprd |
in,found |
R2072 |
T5542 |
T5543 |
det |
an,position |
R2073 |
T5543 |
T5541 |
pobj |
position,in |
R2074 |
T5544 |
T5543 |
advmod |
extreme,position |
R2075 |
T5545 |
T5543 |
amod |
lateral,position |
R2076 |
T5546 |
T5543 |
prep |
at,position |
R2077 |
T5547 |
T5548 |
det |
the,zone |
R2078 |
T5548 |
T5546 |
pobj |
zone,at |
R2079 |
T5549 |
T5550 |
amod |
dorsal,root |
R2080 |
T5550 |
T5548 |
compound |
root,zone |
R2081 |
T5551 |
T5548 |
compound |
entry,zone |
R2082 |
T5552 |
T5539 |
punct |
", ",found |
R2083 |
T5553 |
T5554 |
det |
a,phenotype |
R2084 |
T5554 |
T5539 |
npadvmod |
phenotype,found |
R2085 |
T5555 |
T5554 |
acl |
observed,phenotype |
R2086 |
T5556 |
T5557 |
advmod |
at,least |
R2087 |
T5557 |
T5558 |
advmod |
least,up |
R2088 |
T5558 |
T5555 |
prep |
up,observed |
R2089 |
T5559 |
T5558 |
prep |
to,up |
R2090 |
T5560 |
T5559 |
pobj |
E18.5,to |
R2091 |
T5561 |
T5562 |
punct |
(,3A |
R2092 |
T5562 |
T5539 |
parataxis |
3A,found |
R2093 |
T5563 |
T5562 |
compound |
Figure,3A |
R2094 |
T5564 |
T5565 |
punct |
–,3C |
R2095 |
T5565 |
T5562 |
prep |
3C,3A |
R2096 |
T5566 |
T5562 |
cc |
and,3A |
R2097 |
T5567 |
T5562 |
conj |
3G,3A |
R2098 |
T5568 |
T5569 |
punct |
–,3I |
R2099 |
T5569 |
T5567 |
prep |
3I,3G |
R2100 |
T5570 |
T5562 |
punct |
;,3A |
R2101 |
T5571 |
T5572 |
nsubj |
data,shown |
R2102 |
T5572 |
T5562 |
ccomp |
shown,3A |
R2103 |
T5573 |
T5572 |
neg |
not,shown |
R2104 |
T5574 |
T5562 |
punct |
),3A |
R2105 |
T5575 |
T5502 |
punct |
.,failed |
R2106 |
T5577 |
T5578 |
nsubj |
We,visualized |
R2107 |
T5579 |
T5578 |
advmod |
next,visualized |
R2108 |
T5580 |
T5581 |
det |
the,path |
R2109 |
T5581 |
T5578 |
dobj |
path,visualized |
R2110 |
T5582 |
T5581 |
prep |
of,path |
R2111 |
T5583 |
T5584 |
amod |
sensory,afferent |
R2112 |
T5584 |
T5585 |
nmod |
afferent,projections |
R2113 |
T5585 |
T5582 |
pobj |
projections,of |
R2114 |
T5586 |
T5585 |
prep |
towards,projections |
R2115 |
T5587 |
T5588 |
det |
the,zone |
R2116 |
T5588 |
T5586 |
pobj |
zone,towards |
R2117 |
T5589 |
T5590 |
amod |
dorsal,root |
R2118 |
T5590 |
T5588 |
compound |
root,zone |
R2119 |
T5591 |
T5588 |
compound |
entry,zone |
R2120 |
T5592 |
T5578 |
prep |
in,visualized |
R2121 |
T5593 |
T5594 |
nmod |
TauEWS,Isl1Cre |
R2122 |
T5594 |
T5599 |
nmod |
Isl1Cre,embryos |
R2123 |
T5595 |
T5594 |
punct |
-,Isl1Cre |
R2124 |
T5596 |
T5594 |
nmod |
Pea3,Isl1Cre |
R2125 |
T5597 |
T5594 |
punct |
/,Isl1Cre |
R2126 |
T5598 |
T5594 |
punct |
+,Isl1Cre |
R2127 |
T5599 |
T5592 |
pobj |
embryos,in |
R2128 |
T5600 |
T5594 |
punct |
/,Isl1Cre |
R2129 |
T5601 |
T5594 |
punct |
+,Isl1Cre |
R2130 |
T5602 |
T5578 |
prep |
by,visualized |
R2131 |
T5603 |
T5602 |
pcomp |
injecting,by |
R2132 |
T5604 |
T5605 |
advmod |
fluorescently,labeled |
R2133 |
T5605 |
T5606 |
amod |
labeled,dextran |
R2134 |
T5606 |
T5603 |
dobj |
dextran,injecting |
R2135 |
T5607 |
T5603 |
prep |
into,injecting |
R2136 |
T5608 |
T5609 |
det |
an,DRG |
R2137 |
T5609 |
T5607 |
pobj |
DRG,into |
R2138 |
T5610 |
T5609 |
amod |
individual,DRG |
R2139 |
T5611 |
T5612 |
punct |
(,3M |
R2140 |
T5612 |
T5578 |
parataxis |
3M,visualized |
R2141 |
T5613 |
T5612 |
dep |
L3,3M |
R2142 |
T5614 |
T5612 |
punct |
;,3M |
R2143 |
T5615 |
T5616 |
nsubj |
n,3 |
R2144 |
T5616 |
T5612 |
ccomp |
3,3M |
R2145 |
T5617 |
T5616 |
punct |
=,3 |
R2146 |
T5618 |
T5612 |
punct |
;,3M |
R2147 |
T5619 |
T5612 |
compound |
Figure,3M |
R2148 |
T5620 |
T5621 |
punct |
–,3Q |
R2149 |
T5621 |
T5612 |
prep |
3Q,3M |
R2150 |
T5622 |
T5612 |
punct |
),3M |
R2151 |
T5623 |
T5578 |
punct |
.,visualized |
R2152 |
T5625 |
T5626 |
amod |
Sensory,afferents |
R2153 |
T5626 |
T5627 |
nsubj |
afferents,bifurcated |
R2154 |
T5628 |
T5626 |
prep |
in,afferents |
R2155 |
T5629 |
T5630 |
nmod |
E13.5,embryos |
R2156 |
T5630 |
T5628 |
pobj |
embryos,in |
R2157 |
T5631 |
T5632 |
amod |
wild,type |
R2158 |
T5632 |
T5630 |
compound |
type,embryos |
R2159 |
T5633 |
T5632 |
punct |
-,type |
R2160 |
T5634 |
T5627 |
prep |
at,bifurcated |
R2161 |
T5635 |
T5636 |
poss |
their,point |
R2162 |
T5636 |
T5634 |
pobj |
point,at |
R2163 |
T5637 |
T5636 |
amod |
lateral,point |
R2164 |
T5638 |
T5636 |
amod |
spinal,point |
R2165 |
T5639 |
T5636 |
compound |
entry,point |
R2166 |
T5640 |
T5627 |
punct |
", ",bifurcated |
R2167 |
T5641 |
T5627 |
cc |
and,bifurcated |
R2168 |
T5642 |
T5627 |
conj |
projected,bifurcated |
R2169 |
T5643 |
T5642 |
advmod |
rostrally,projected |
R2170 |
T5644 |
T5643 |
cc |
and,rostrally |
R2171 |
T5645 |
T5643 |
conj |
caudally,rostrally |
R2172 |
T5646 |
T5642 |
prep |
over,projected |
R2173 |
T5647 |
T5648 |
nummod |
six,levels |
R2174 |
T5648 |
T5646 |
pobj |
levels,over |
R2175 |
T5649 |
T5647 |
cc |
or,six |
R2176 |
T5650 |
T5647 |
conj |
more,six |
R2177 |
T5651 |
T5648 |
amod |
segmental,levels |
R2178 |
T5652 |
T5653 |
mark |
while,approaching |
R2179 |
T5653 |
T5627 |
advcl |
approaching,bifurcated |
R2180 |
T5654 |
T5653 |
advmod |
gradually,approaching |
R2181 |
T5655 |
T5656 |
det |
the,midline |
R2182 |
T5656 |
T5653 |
dobj |
midline,approaching |
R2183 |
T5657 |
T5658 |
punct |
(,3M |
R2184 |
T5658 |
T5653 |
parataxis |
3M,approaching |
R2185 |
T5659 |
T5658 |
compound |
Figure,3M |
R2186 |
T5660 |
T5658 |
punct |
),3M |
R2187 |
T5661 |
T5627 |
punct |
.,bifurcated |
R2188 |
T5663 |
T5664 |
amod |
Sensory,afferents |
R2189 |
T5664 |
T5665 |
nsubj |
afferents,bifurcated |
R2190 |
T5666 |
T5664 |
prep |
in,afferents |
R2191 |
T5667 |
T5668 |
nmod |
TauEWS,Isl1Cre |
R2192 |
T5668 |
T5673 |
nmod |
Isl1Cre,embryos |
R2193 |
T5669 |
T5668 |
punct |
-,Isl1Cre |
R2194 |
T5670 |
T5668 |
nmod |
Pea3,Isl1Cre |
R2195 |
T5671 |
T5668 |
punct |
/,Isl1Cre |
R2196 |
T5672 |
T5668 |
punct |
+,Isl1Cre |
R2197 |
T5673 |
T5666 |
pobj |
embryos,in |
R2198 |
T5674 |
T5668 |
punct |
/,Isl1Cre |
R2199 |
T5675 |
T5668 |
punct |
+,Isl1Cre |
R2200 |
T5676 |
T5665 |
advmod |
also,bifurcated |
R2201 |
T5677 |
T5665 |
prep |
at,bifurcated |
R2202 |
T5678 |
T5679 |
det |
the,point |
R2203 |
T5679 |
T5677 |
pobj |
point,at |
R2204 |
T5680 |
T5679 |
compound |
entry,point |
R2205 |
T5681 |
T5665 |
punct |
", ",bifurcated |
R2206 |
T5682 |
T5683 |
mark |
although,continued |
R2207 |
T5683 |
T5665 |
advcl |
continued,bifurcated |
R2208 |
T5684 |
T5685 |
advmod |
approximately,5 |
R2209 |
T5685 |
T5686 |
nummod |
5,% |
R2210 |
T5686 |
T5683 |
nsubj |
%,continued |
R2211 |
T5687 |
T5686 |
prep |
of,% |
R2212 |
T5688 |
T5689 |
amod |
afferent,fibers |
R2213 |
T5689 |
T5687 |
pobj |
fibers,of |
R2214 |
T5690 |
T5691 |
aux |
to,grow |
R2215 |
T5691 |
T5683 |
xcomp |
grow,continued |
R2216 |
T5692 |
T5691 |
prep |
towards,grow |
R2217 |
T5693 |
T5694 |
det |
the,midline |
R2218 |
T5694 |
T5692 |
pobj |
midline,towards |
R2219 |
T5695 |
T5696 |
punct |
(,3O |
R2220 |
T5696 |
T5665 |
parataxis |
3O,bifurcated |
R2221 |
T5697 |
T5696 |
compound |
Figure,3O |
R2222 |
T5698 |
T5696 |
cc |
and,3O |
R2223 |
T5699 |
T5696 |
conj |
3Q,3O |
R2224 |
T5700 |
T5696 |
punct |
),3O |
R2225 |
T5701 |
T5665 |
punct |
.,bifurcated |
R2226 |
T5703 |
T5704 |
mark |
While,were |
R2227 |
T5704 |
T5709 |
advcl |
were,failed |
R2228 |
T5705 |
T5706 |
amod |
rostro,caudal |
R2229 |
T5706 |
T5708 |
amod |
caudal,projections |
R2230 |
T5707 |
T5706 |
punct |
-,caudal |
R2231 |
T5708 |
T5704 |
nsubj |
projections,were |
R2232 |
T5710 |
T5704 |
acomp |
present,were |
R2233 |
T5711 |
T5704 |
prep |
in,were |
R2234 |
T5712 |
T5713 |
nmod |
TauEWS,Isl1Cre |
R2235 |
T5713 |
T5718 |
nmod |
Isl1Cre,embryos |
R2236 |
T5714 |
T5713 |
punct |
-,Isl1Cre |
R2237 |
T5715 |
T5713 |
nmod |
Pea3,Isl1Cre |
R2238 |
T5716 |
T5713 |
punct |
/,Isl1Cre |
R2239 |
T5717 |
T5713 |
punct |
+,Isl1Cre |
R2240 |
T5718 |
T5711 |
pobj |
embryos,in |
R2241 |
T5719 |
T5713 |
punct |
/,Isl1Cre |
R2242 |
T5720 |
T5713 |
punct |
+,Isl1Cre |
R2243 |
T5721 |
T5709 |
punct |
", ",failed |
R2244 |
T5722 |
T5723 |
amod |
afferent,fibers |
R2245 |
T5723 |
T5709 |
nsubj |
fibers,failed |
R2246 |
T5724 |
T5725 |
aux |
to,approach |
R2247 |
T5725 |
T5709 |
xcomp |
approach,failed |
R2248 |
T5726 |
T5727 |
det |
the,midline |
R2249 |
T5727 |
T5725 |
dobj |
midline,approach |
R2250 |
T5728 |
T5725 |
prep |
at,approach |
R2251 |
T5729 |
T5730 |
amod |
distal,segments |
R2252 |
T5730 |
T5728 |
pobj |
segments,at |
R2253 |
T5731 |
T5709 |
cc |
and,failed |
R2254 |
T5732 |
T5709 |
conj |
continued,failed |
R2255 |
T5733 |
T5734 |
aux |
to,occupy |
R2256 |
T5734 |
T5732 |
xcomp |
occupy,continued |
R2257 |
T5735 |
T5736 |
det |
an,position |
R2258 |
T5736 |
T5734 |
dobj |
position,occupy |
R2259 |
T5737 |
T5736 |
advmod |
extreme,position |
R2260 |
T5738 |
T5736 |
amod |
lateral,position |
R2261 |
T5739 |
T5740 |
punct |
(,3O |
R2262 |
T5740 |
T5736 |
parataxis |
3O,position |
R2263 |
T5741 |
T5740 |
compound |
Figure,3O |
R2264 |
T5742 |
T5740 |
punct |
),3O |
R2265 |
T5743 |
T5734 |
punct |
", ",occupy |
R2266 |
T5744 |
T5734 |
advcl |
consistent,occupy |
R2267 |
T5745 |
T5744 |
prep |
with,consistent |
R2268 |
T5746 |
T5747 |
det |
the,analysis |
R2269 |
T5747 |
T5745 |
pobj |
analysis,with |
R2270 |
T5748 |
T5747 |
prep |
of,analysis |
R2271 |
T5749 |
T5750 |
amod |
transverse,sections |
R2272 |
T5750 |
T5748 |
pobj |
sections,of |
R2273 |
T5751 |
T5709 |
punct |
.,failed |
R2274 |
T5753 |
T5754 |
nsubj |
We,examined |
R2275 |
T5755 |
T5754 |
advmod |
next,examined |
R2276 |
T5756 |
T5757 |
det |
the,establishment |
R2277 |
T5757 |
T5754 |
dobj |
establishment,examined |
R2278 |
T5758 |
T5757 |
prep |
of,establishment |
R2279 |
T5759 |
T5760 |
amod |
peripheral,projections |
R2280 |
T5760 |
T5758 |
pobj |
projections,of |
R2281 |
T5761 |
T5760 |
prep |
upon,projections |
R2282 |
T5762 |
T5763 |
amod |
precocious,expression |
R2283 |
T5763 |
T5761 |
pobj |
expression,upon |
R2284 |
T5764 |
T5765 |
compound |
EWS,Pea3 |
R2285 |
T5765 |
T5763 |
compound |
Pea3,expression |
R2286 |
T5766 |
T5765 |
punct |
-,Pea3 |
R2287 |
T5767 |
T5754 |
prep |
in,examined |
R2288 |
T5768 |
T5769 |
compound |
DRG,neurons |
R2289 |
T5769 |
T5767 |
pobj |
neurons,in |
R2290 |
T5770 |
T5754 |
punct |
.,examined |
R2291 |
T5772 |
T5773 |
mark |
While,reached |
R2292 |
T5773 |
T5786 |
advcl |
reached,established |
R2293 |
T5774 |
T5775 |
amod |
sensory,axons |
R2294 |
T5775 |
T5773 |
nsubj |
axons,reached |
R2295 |
T5776 |
T5775 |
prep |
in,axons |
R2296 |
T5777 |
T5778 |
nmod |
TauEWS,Isl1Cre |
R2297 |
T5778 |
T5783 |
nmod |
Isl1Cre,embryos |
R2298 |
T5779 |
T5778 |
punct |
-,Isl1Cre |
R2299 |
T5780 |
T5778 |
nmod |
Pea3,Isl1Cre |
R2300 |
T5781 |
T5778 |
punct |
/,Isl1Cre |
R2301 |
T5782 |
T5778 |
punct |
+,Isl1Cre |
R2302 |
T5783 |
T5776 |
pobj |
embryos,in |
R2303 |
T5784 |
T5778 |
punct |
/,Isl1Cre |
R2304 |
T5785 |
T5778 |
punct |
+,Isl1Cre |
R2305 |
T5787 |
T5788 |
det |
the,skin |
R2306 |
T5788 |
T5773 |
dobj |
skin,reached |
R2307 |
T5789 |
T5773 |
cc |
and,reached |
R2308 |
T5790 |
T5773 |
conj |
established,reached |
R2309 |
T5791 |
T5792 |
amod |
major,trunks |
R2310 |
T5792 |
T5790 |
dobj |
trunks,established |
R2311 |
T5793 |
T5792 |
compound |
nerve,trunks |
R2312 |
T5794 |
T5790 |
prep |
by,established |
R2313 |
T5795 |
T5794 |
pobj |
E16.5,by |
R2314 |
T5796 |
T5786 |
punct |
", ",established |
R2315 |
T5797 |
T5798 |
advmod |
only,branching |
R2316 |
T5798 |
T5786 |
nsubjpass |
branching,established |
R2317 |
T5799 |
T5798 |
amod |
rudimentary,branching |
R2318 |
T5800 |
T5801 |
amod |
sensory,axon |
R2319 |
T5801 |
T5798 |
compound |
axon,branching |
R2320 |
T5802 |
T5786 |
auxpass |
was,established |
R2321 |
T5803 |
T5786 |
prep |
within,established |
R2322 |
T5804 |
T5805 |
det |
the,skin |
R2323 |
T5805 |
T5803 |
pobj |
skin,within |
R2324 |
T5806 |
T5807 |
punct |
(,3D |
R2325 |
T5807 |
T5786 |
parataxis |
3D,established |
R2326 |
T5808 |
T5807 |
compound |
Figure,3D |
R2327 |
T5809 |
T5807 |
cc |
and,3D |
R2328 |
T5810 |
T5807 |
conj |
3J,3D |
R2329 |
T5811 |
T5807 |
punct |
),3D |
R2330 |
T5812 |
T5786 |
punct |
.,established |
R2331 |
T5814 |
T5815 |
prep |
In,was |
R2332 |
T5816 |
T5814 |
pobj |
addition,In |
R2333 |
T5817 |
T5815 |
punct |
", ",was |
R2334 |
T5818 |
T5815 |
expl |
there,was |
R2335 |
T5819 |
T5820 |
det |
a,reduction |
R2336 |
T5820 |
T5815 |
attr |
reduction,was |
R2337 |
T5821 |
T5820 |
amod |
significant,reduction |
R2338 |
T5822 |
T5820 |
prep |
in,reduction |
R2339 |
T5823 |
T5824 |
det |
the,number |
R2340 |
T5824 |
T5822 |
pobj |
number,in |
R2341 |
T5825 |
T5824 |
prep |
of,number |
R2342 |
T5826 |
T5827 |
compound |
muscle,spindles |
R2343 |
T5827 |
T5825 |
pobj |
spindles,of |
R2344 |
T5828 |
T5827 |
prep |
in,spindles |
R2345 |
T5829 |
T5830 |
nmod |
TauEWS,Isl1Cre |
R2346 |
T5830 |
T5835 |
nmod |
Isl1Cre,embryos |
R2347 |
T5831 |
T5830 |
punct |
-,Isl1Cre |
R2348 |
T5832 |
T5830 |
nmod |
Pea3,Isl1Cre |
R2349 |
T5833 |
T5830 |
punct |
/,Isl1Cre |
R2350 |
T5834 |
T5830 |
punct |
+,Isl1Cre |
R2351 |
T5835 |
T5828 |
pobj |
embryos,in |
R2352 |
T5836 |
T5830 |
punct |
/,Isl1Cre |
R2353 |
T5837 |
T5830 |
punct |
+,Isl1Cre |
R2354 |
T5838 |
T5839 |
punct |
(,% |
R2355 |
T5839 |
T5824 |
parataxis |
%,number |
R2356 |
T5840 |
T5841 |
advmod |
approximately,25 |
R2357 |
T5841 |
T5839 |
nummod |
25,% |
R2358 |
T5842 |
T5839 |
prep |
of,% |
R2359 |
T5843 |
T5844 |
amod |
wild,type |
R2360 |
T5844 |
T5846 |
compound |
type,complement |
R2361 |
T5845 |
T5844 |
punct |
-,type |
R2362 |
T5846 |
T5842 |
pobj |
complement,of |
R2363 |
T5847 |
T5839 |
punct |
;,% |
R2364 |
T5848 |
T5849 |
nsubj |
n,3 |
R2365 |
T5849 |
T5839 |
ccomp |
3,% |
R2366 |
T5850 |
T5849 |
punct |
=,3 |
R2367 |
T5851 |
T5839 |
punct |
),% |
R2368 |
T5852 |
T5853 |
mark |
as,assessed |
R2369 |
T5853 |
T5815 |
advcl |
assessed,was |
R2370 |
T5854 |
T5853 |
prep |
by,assessed |
R2371 |
T5855 |
T5854 |
pobj |
innervation,by |
R2372 |
T5856 |
T5855 |
cc |
and,innervation |
R2373 |
T5857 |
T5855 |
conj |
expression,innervation |
R2374 |
T5858 |
T5855 |
prep |
of,innervation |
R2375 |
T5859 |
T5858 |
pobj |
genes,of |
R2376 |
T5860 |
T5859 |
amod |
specific,genes |
R2377 |
T5861 |
T5860 |
prep |
for,specific |
R2378 |
T5862 |
T5863 |
amod |
intrafusal,fibers |
R2379 |
T5863 |
T5861 |
pobj |
fibers,for |
R2380 |
T5864 |
T5863 |
compound |
muscle,fibers |
R2381 |
T5865 |
T5866 |
amod |
such,as |
R2382 |
T5866 |
T5863 |
prep |
as,fibers |
R2383 |
T5867 |
T5866 |
pobj |
Egr3,as |
R2384 |
T5868 |
T5869 |
punct |
(,26 |
R2385 |
T5869 |
T5853 |
parataxis |
26,assessed |
R2386 |
T5870 |
T5871 |
compound |
Figure,3E |
R2387 |
T5871 |
T5869 |
dep |
3E,26 |
R2388 |
T5872 |
T5871 |
punct |
", ",3E |
R2389 |
T5873 |
T5871 |
conj |
3F,3E |
R2390 |
T5874 |
T5873 |
punct |
", ",3F |
R2391 |
T5875 |
T5873 |
conj |
3K,3F |
R2392 |
T5876 |
T5875 |
punct |
", ",3K |
R2393 |
T5877 |
T5875 |
cc |
and,3K |
R2394 |
T5878 |
T5875 |
conj |
3L,3K |
R2395 |
T5879 |
T5869 |
punct |
;,26 |
R2396 |
T5880 |
T5869 |
punct |
[,26 |
R2397 |
T5881 |
T5869 |
punct |
],26 |
R2398 |
T5882 |
T5869 |
punct |
),26 |
R2399 |
T5883 |
T5815 |
punct |
.,was |
R2400 |
T5885 |
T5886 |
prep |
In,interfered |
R2401 |
T5887 |
T5885 |
pobj |
summary,In |
R2402 |
T5888 |
T5886 |
punct |
", ",interfered |
R2403 |
T5889 |
T5890 |
mark |
whereas,promoted |
R2404 |
T5890 |
T5886 |
advcl |
promoted,interfered |
R2405 |
T5891 |
T5892 |
amod |
isochronic,expression |
R2406 |
T5892 |
T5890 |
nsubj |
expression,promoted |
R2407 |
T5893 |
T5892 |
prep |
of,expression |
R2408 |
T5894 |
T5895 |
compound |
EWS,Pea3 |
R2409 |
T5895 |
T5893 |
pobj |
Pea3,of |
R2410 |
T5896 |
T5895 |
punct |
-,Pea3 |
R2411 |
T5897 |
T5898 |
det |
the,establishment |
R2412 |
T5898 |
T5890 |
dobj |
establishment,promoted |
R2413 |
T5899 |
T5898 |
prep |
of,establishment |
R2414 |
T5900 |
T5901 |
amod |
proprioceptive,projections |
R2415 |
T5901 |
T5899 |
pobj |
projections,of |
R2416 |
T5902 |
T5901 |
amod |
afferent,projections |
R2417 |
T5903 |
T5901 |
prep |
into,projections |
R2418 |
T5904 |
T5905 |
det |
the,cord |
R2419 |
T5905 |
T5903 |
pobj |
cord,into |
R2420 |
T5906 |
T5905 |
amod |
ventral,cord |
R2421 |
T5907 |
T5905 |
amod |
spinal,cord |
R2422 |
T5908 |
T5886 |
punct |
", ",interfered |
R2423 |
T5909 |
T5910 |
amod |
precocious,expression |
R2424 |
T5910 |
T5886 |
nsubj |
expression,interfered |
R2425 |
T5911 |
T5910 |
prep |
of,expression |
R2426 |
T5912 |
T5913 |
det |
the,factor |
R2427 |
T5913 |
T5911 |
pobj |
factor,of |
R2428 |
T5914 |
T5913 |
amod |
same,factor |
R2429 |
T5915 |
T5913 |
compound |
ETS,factor |
R2430 |
T5916 |
T5913 |
compound |
signaling,factor |
R2431 |
T5917 |
T5910 |
prep |
in,expression |
R2432 |
T5918 |
T5919 |
compound |
DRG,neurons |
R2433 |
T5919 |
T5917 |
pobj |
neurons,in |
R2434 |
T5920 |
T5886 |
prep |
with,interfered |
R2435 |
T5921 |
T5920 |
pobj |
establishment,with |
R2436 |
T5922 |
T5921 |
prep |
of,establishment |
R2437 |
T5923 |
T5922 |
pobj |
projections,of |
R2438 |
T5924 |
T5923 |
prep |
into,projections |
R2439 |
T5925 |
T5926 |
det |
the,cord |
R2440 |
T5926 |
T5924 |
pobj |
cord,into |
R2441 |
T5927 |
T5926 |
amod |
spinal,cord |
R2442 |
T5928 |
T5929 |
advmod |
as,as |
R2443 |
T5929 |
T5924 |
cc |
as,into |
R2444 |
T5930 |
T5929 |
advmod |
well,as |
R2445 |
T5931 |
T5924 |
conj |
to,into |
R2446 |
T5932 |
T5933 |
amod |
peripheral,targets |
R2447 |
T5933 |
T5931 |
pobj |
targets,to |
R2448 |
T5934 |
T5886 |
punct |
.,interfered |
R2451 |
T6663 |
T6664 |
amod |
Precocious,Expression |
R2452 |
T6664 |
T6668 |
nsubj |
Expression,Promotes |
R2453 |
T6665 |
T6666 |
compound |
EWS,Pea3 |
R2454 |
T6666 |
T6664 |
compound |
Pea3,Expression |
R2455 |
T6667 |
T6666 |
punct |
-,Pea3 |
R2456 |
T6669 |
T6670 |
npadvmod |
Neurotrophin,Independent |
R2457 |
T6670 |
T6672 |
amod |
Independent,Survival |
R2458 |
T6671 |
T6670 |
punct |
-,Independent |
R2459 |
T6672 |
T6668 |
dobj |
Survival,Promotes |
R2460 |
T6673 |
T6672 |
cc |
and,Survival |
R2461 |
T6674 |
T6675 |
compound |
Neurite,Outgrowth |
R2462 |
T6675 |
T6672 |
conj |
Outgrowth,Survival |
R2463 |
T6677 |
T6678 |
aux |
To,begin |
R2464 |
T6678 |
T6679 |
advcl |
begin,turned |
R2465 |
T6680 |
T6681 |
aux |
to,address |
R2466 |
T6681 |
T6678 |
xcomp |
address,begin |
R2467 |
T6682 |
T6683 |
det |
the,mechanisms |
R2468 |
T6683 |
T6681 |
dobj |
mechanisms,address |
R2469 |
T6684 |
T6683 |
amod |
cellular,mechanisms |
R2470 |
T6685 |
T6684 |
cc |
and,cellular |
R2471 |
T6686 |
T6684 |
conj |
molecular,cellular |
R2472 |
T6687 |
T6683 |
acl |
involved,mechanisms |
R2473 |
T6688 |
T6687 |
prep |
in,involved |
R2474 |
T6689 |
T6690 |
det |
the,actions |
R2475 |
T6690 |
T6688 |
pobj |
actions,in |
R2476 |
T6691 |
T6690 |
amod |
distinct,actions |
R2477 |
T6692 |
T6690 |
amod |
biological,actions |
R2478 |
T6693 |
T6690 |
prep |
of,actions |
R2479 |
T6694 |
T6695 |
compound |
EWS,Pea3 |
R2480 |
T6695 |
T6693 |
pobj |
Pea3,of |
R2481 |
T6696 |
T6695 |
punct |
-,Pea3 |
R2482 |
T6697 |
T6687 |
prep |
at,involved |
R2483 |
T6698 |
T6699 |
amod |
different,stages |
R2484 |
T6699 |
T6697 |
pobj |
stages,at |
R2485 |
T6700 |
T6699 |
amod |
developmental,stages |
R2486 |
T6701 |
T6679 |
punct |
", ",turned |
R2487 |
T6702 |
T6679 |
nsubj |
we,turned |
R2488 |
T6703 |
T6679 |
advmod |
first,turned |
R2489 |
T6704 |
T6679 |
prep |
to,turned |
R2490 |
T6705 |
T6706 |
advmod |
in,vitro |
R2491 |
T6706 |
T6707 |
amod |
vitro,experiments |
R2492 |
T6707 |
T6704 |
pobj |
experiments,to |
R2493 |
T6708 |
T6707 |
compound |
culture,experiments |
R2494 |
T6709 |
T6679 |
punct |
.,turned |
R2495 |
T6711 |
T6712 |
det |
These,experiments |
R2496 |
T6712 |
T6713 |
nsubj |
experiments,permit |
R2497 |
T6714 |
T6713 |
dobj |
assessment,permit |
R2498 |
T6715 |
T6714 |
prep |
of,assessment |
R2499 |
T6716 |
T6717 |
mark |
whether,influences |
R2500 |
T6717 |
T6715 |
pcomp |
influences,of |
R2501 |
T6718 |
T6719 |
amod |
precocious,signaling |
R2502 |
T6719 |
T6717 |
nsubj |
signaling,influences |
R2503 |
T6720 |
T6719 |
compound |
ETS,signaling |
R2504 |
T6721 |
T6722 |
compound |
transcription,factor |
R2505 |
T6722 |
T6719 |
compound |
factor,signaling |
R2506 |
T6723 |
T6724 |
amod |
neuronal,survival |
R2507 |
T6724 |
T6717 |
dobj |
survival,influences |
R2508 |
T6725 |
T6724 |
cc |
and,survival |
R2509 |
T6726 |
T6727 |
nmod |
in,vitro |
R2510 |
T6727 |
T6728 |
nmod |
vitro,outgrowth |
R2511 |
T6728 |
T6724 |
conj |
outgrowth,survival |
R2512 |
T6729 |
T6728 |
compound |
neurite,outgrowth |
R2513 |
T6730 |
T6724 |
prep |
of,survival |
R2514 |
T6731 |
T6732 |
compound |
DRG,neurons |
R2515 |
T6732 |
T6730 |
pobj |
neurons,of |
R2516 |
T6733 |
T6724 |
punct |
", ",survival |
R2517 |
T6734 |
T6735 |
nummod |
two,parameters |
R2518 |
T6735 |
T6724 |
appos |
parameters,survival |
R2519 |
T6736 |
T6737 |
advmod |
prominently,influenced |
R2520 |
T6737 |
T6735 |
acl |
influenced,parameters |
R2521 |
T6738 |
T6737 |
agent |
by,influenced |
R2522 |
T6739 |
T6740 |
npadvmod |
target,derived |
R2523 |
T6740 |
T6742 |
amod |
derived,factors |
R2524 |
T6741 |
T6740 |
punct |
-,derived |
R2525 |
T6742 |
T6738 |
pobj |
factors,by |
R2526 |
T6743 |
T6742 |
amod |
neurotrophic,factors |
R2527 |
T6744 |
T6742 |
cc |
and,factors |
R2528 |
T6745 |
T6746 |
poss |
their,receptors |
R2529 |
T6746 |
T6742 |
conj |
receptors,factors |
R2530 |
T6747 |
T6713 |
punct |
.,permit |
R2531 |
T6749 |
T6750 |
nsubj |
We,cultured |
R2532 |
T6751 |
T6752 |
nmod |
E13.5,explants |
R2533 |
T6752 |
T6750 |
dobj |
explants,cultured |
R2534 |
T6753 |
T6752 |
amod |
whole,explants |
R2535 |
T6754 |
T6752 |
compound |
DRG,explants |
R2536 |
T6755 |
T6752 |
prep |
from,explants |
R2537 |
T6756 |
T6757 |
amod |
wild,type |
R2538 |
T6757 |
T6759 |
nmod |
type,embryos |
R2539 |
T6758 |
T6757 |
punct |
-,type |
R2540 |
T6759 |
T6755 |
pobj |
embryos,from |
R2541 |
T6760 |
T6757 |
cc |
and,type |
R2542 |
T6761 |
T6762 |
nmod |
TauEWS,Isl1Cre |
R2543 |
T6762 |
T6757 |
conj |
Isl1Cre,type |
R2544 |
T6763 |
T6762 |
punct |
-,Isl1Cre |
R2545 |
T6764 |
T6762 |
nmod |
Pea3,Isl1Cre |
R2546 |
T6765 |
T6762 |
punct |
/,Isl1Cre |
R2547 |
T6766 |
T6762 |
punct |
+,Isl1Cre |
R2548 |
T6767 |
T6762 |
punct |
/,Isl1Cre |
R2549 |
T6768 |
T6762 |
punct |
+,Isl1Cre |
R2550 |
T6769 |
T6752 |
prep |
in,explants |
R2551 |
T6770 |
T6771 |
det |
the,presence |
R2552 |
T6771 |
T6769 |
pobj |
presence,in |
R2553 |
T6772 |
T6771 |
prep |
of,presence |
R2554 |
T6773 |
T6772 |
pobj |
NGF,of |
R2555 |
T6774 |
T6773 |
cc |
or,NGF |
R2556 |
T6775 |
T6773 |
conj |
NT,NGF |
R2557 |
T6776 |
T6775 |
punct |
-,NT |
R2558 |
T6777 |
T6775 |
nummod |
3,NT |
R2559 |
T6778 |
T6769 |
cc |
or,in |
R2560 |
T6779 |
T6769 |
conj |
in,in |
R2561 |
T6780 |
T6781 |
det |
the,absence |
R2562 |
T6781 |
T6779 |
pobj |
absence,in |
R2563 |
T6782 |
T6781 |
prep |
of,absence |
R2564 |
T6783 |
T6782 |
pobj |
neurotrophins,of |
R2565 |
T6784 |
T6750 |
cc |
and,cultured |
R2566 |
T6785 |
T6750 |
conj |
analyzed,cultured |
R2567 |
T6786 |
T6787 |
amod |
neuronal,survival |
R2568 |
T6787 |
T6785 |
dobj |
survival,analyzed |
R2569 |
T6788 |
T6787 |
cc |
and,survival |
R2570 |
T6789 |
T6790 |
compound |
neurite,outgrowth |
R2571 |
T6790 |
T6787 |
conj |
outgrowth,survival |
R2572 |
T6791 |
T6785 |
prep |
on,analyzed |
R2573 |
T6792 |
T6793 |
compound |
matrigel,substrate |
R2574 |
T6793 |
T6791 |
pobj |
substrate,on |
R2575 |
T6794 |
T6785 |
prep |
after,analyzed |
R2576 |
T6795 |
T6796 |
nummod |
48,h |
R2577 |
T6796 |
T6794 |
pobj |
h,after |
R2578 |
T6797 |
T6798 |
advmod |
in,vitro |
R2579 |
T6798 |
T6785 |
advmod |
vitro,analyzed |
R2580 |
T6799 |
T6750 |
punct |
.,cultured |
R2581 |
T6801 |
T6802 |
prep |
Without,survived |
R2582 |
T6803 |
T6804 |
amod |
neurotrophic,support |
R2583 |
T6804 |
T6801 |
pobj |
support,Without |
R2584 |
T6805 |
T6802 |
punct |
", ",survived |
R2585 |
T6806 |
T6807 |
advmod |
very,few |
R2586 |
T6807 |
T6808 |
amod |
few,neurons |
R2587 |
T6808 |
T6802 |
nsubj |
neurons,survived |
R2588 |
T6809 |
T6810 |
amod |
wild,type |
R2589 |
T6810 |
T6808 |
compound |
type,neurons |
R2590 |
T6811 |
T6810 |
punct |
-,type |
R2591 |
T6812 |
T6808 |
compound |
DRG,neurons |
R2592 |
T6813 |
T6814 |
punct |
(,4A |
R2593 |
T6814 |
T6802 |
parataxis |
4A,survived |
R2594 |
T6815 |
T6814 |
compound |
Figure,4A |
R2595 |
T6816 |
T6814 |
punct |
),4A |
R2596 |
T6817 |
T6802 |
punct |
.,survived |
R2597 |
T6819 |
T6820 |
prep |
In,led |
R2598 |
T6821 |
T6819 |
pobj |
contrast,In |
R2599 |
T6822 |
T6820 |
punct |
", ",led |
R2600 |
T6823 |
T6820 |
csubj |
culturing,led |
R2601 |
T6824 |
T6825 |
amod |
wild,type |
R2602 |
T6825 |
T6827 |
compound |
type,DRG |
R2603 |
T6826 |
T6825 |
punct |
-,type |
R2604 |
T6827 |
T6823 |
dobj |
DRG,culturing |
R2605 |
T6828 |
T6823 |
prep |
with,culturing |
R2606 |
T6829 |
T6830 |
amod |
neurotrophic,factors |
R2607 |
T6830 |
T6828 |
pobj |
factors,with |
R2608 |
T6831 |
T6820 |
prep |
to,led |
R2609 |
T6832 |
T6833 |
amod |
neuronal,survival |
R2610 |
T6833 |
T6831 |
pobj |
survival,to |
R2611 |
T6834 |
T6833 |
cc |
and,survival |
R2612 |
T6835 |
T6836 |
compound |
neurite,outgrowth |
R2613 |
T6836 |
T6833 |
conj |
outgrowth,survival |
R2614 |
T6837 |
T6820 |
punct |
.,led |
R2615 |
T6839 |
T6840 |
nsubj |
Addition,resulted |
R2616 |
T6841 |
T6839 |
prep |
of,Addition |
R2617 |
T6842 |
T6841 |
pobj |
NGF,of |
R2618 |
T6843 |
T6842 |
punct |
", ",NGF |
R2619 |
T6844 |
T6845 |
dep |
which,supports |
R2620 |
T6845 |
T6842 |
relcl |
supports,NGF |
R2621 |
T6846 |
T6845 |
dobj |
survival,supports |
R2622 |
T6847 |
T6846 |
prep |
of,survival |
R2623 |
T6848 |
T6849 |
amod |
cutaneous,afferents |
R2624 |
T6849 |
T6847 |
pobj |
afferents,of |
R2625 |
T6850 |
T6840 |
punct |
", ",resulted |
R2626 |
T6851 |
T6840 |
prep |
in,resulted |
R2627 |
T6852 |
T6853 |
amod |
straight,outgrowth |
R2628 |
T6853 |
T6851 |
pobj |
outgrowth,in |
R2629 |
T6854 |
T6852 |
cc |
and,straight |
R2630 |
T6855 |
T6852 |
conj |
unbranched,straight |
R2631 |
T6856 |
T6853 |
compound |
neurite,outgrowth |
R2632 |
T6857 |
T6858 |
punct |
(,4B |
R2633 |
T6858 |
T6853 |
parataxis |
4B,outgrowth |
R2634 |
T6859 |
T6858 |
compound |
Figure,4B |
R2635 |
T6860 |
T6858 |
punct |
),4B |
R2636 |
T6861 |
T6840 |
punct |
", ",resulted |
R2637 |
T6862 |
T6863 |
mark |
while,resulted |
R2638 |
T6863 |
T6840 |
advcl |
resulted,resulted |
R2639 |
T6864 |
T6863 |
nsubj |
cultures,resulted |
R2640 |
T6865 |
T6864 |
acl |
grown,cultures |
R2641 |
T6866 |
T6865 |
prep |
in,grown |
R2642 |
T6867 |
T6868 |
det |
the,presence |
R2643 |
T6868 |
T6866 |
pobj |
presence,in |
R2644 |
T6869 |
T6868 |
prep |
of,presence |
R2645 |
T6870 |
T6869 |
pobj |
NT,of |
R2646 |
T6871 |
T6870 |
punct |
-,NT |
R2647 |
T6872 |
T6870 |
nummod |
3,NT |
R2648 |
T6873 |
T6870 |
punct |
", ",NT |
R2649 |
T6874 |
T6875 |
dep |
which,supports |
R2650 |
T6875 |
T6870 |
relcl |
supports,NT |
R2651 |
T6876 |
T6875 |
dobj |
survival,supports |
R2652 |
T6877 |
T6876 |
prep |
of,survival |
R2653 |
T6878 |
T6879 |
amod |
proprioceptive,afferents |
R2654 |
T6879 |
T6877 |
pobj |
afferents,of |
R2655 |
T6880 |
T6863 |
punct |
", ",resulted |
R2656 |
T6881 |
T6863 |
prep |
in,resulted |
R2657 |
T6882 |
T6883 |
det |
a,pattern |
R2658 |
T6883 |
T6881 |
pobj |
pattern,in |
R2659 |
T6884 |
T6885 |
advmod |
highly,branched |
R2660 |
T6885 |
T6883 |
amod |
branched,pattern |
R2661 |
T6886 |
T6887 |
compound |
neurite,outgrowth |
R2662 |
T6887 |
T6883 |
compound |
outgrowth,pattern |
R2663 |
T6888 |
T6863 |
prep |
after,resulted |
R2664 |
T6889 |
T6890 |
nummod |
48,h |
R2665 |
T6890 |
T6888 |
pobj |
h,after |
R2666 |
T6891 |
T6892 |
advmod |
in,vitro |
R2667 |
T6892 |
T6890 |
advmod |
vitro,h |
R2668 |
T6893 |
T6894 |
punct |
(,4C |
R2669 |
T6894 |
T6863 |
parataxis |
4C,resulted |
R2670 |
T6895 |
T6894 |
compound |
Figure,4C |
R2671 |
T6896 |
T6894 |
punct |
),4C |
R2672 |
T6897 |
T6840 |
punct |
.,resulted |
R2673 |
T6899 |
T6900 |
advmod |
Surprisingly,survived |
R2674 |
T6901 |
T6900 |
punct |
", ",survived |
R2675 |
T6902 |
T6903 |
compound |
DRG,neurons |
R2676 |
T6903 |
T6900 |
nsubj |
neurons,survived |
R2677 |
T6904 |
T6903 |
acl |
isolated,neurons |
R2678 |
T6905 |
T6904 |
prep |
from,isolated |
R2679 |
T6906 |
T6907 |
nmod |
TauEWS,Isl1Cre |
R2680 |
T6907 |
T6912 |
nmod |
Isl1Cre,embryos |
R2681 |
T6908 |
T6907 |
punct |
-,Isl1Cre |
R2682 |
T6909 |
T6907 |
nmod |
Pea3,Isl1Cre |
R2683 |
T6910 |
T6907 |
punct |
/,Isl1Cre |
R2684 |
T6911 |
T6907 |
punct |
+,Isl1Cre |
R2685 |
T6912 |
T6905 |
pobj |
embryos,from |
R2686 |
T6913 |
T6907 |
punct |
/,Isl1Cre |
R2687 |
T6914 |
T6907 |
punct |
+,Isl1Cre |
R2688 |
T6915 |
T6904 |
cc |
and,isolated |
R2689 |
T6916 |
T6904 |
conj |
cultured,isolated |
R2690 |
T6917 |
T6916 |
prep |
without,cultured |
R2691 |
T6918 |
T6919 |
amod |
neurotrophic,support |
R2692 |
T6919 |
T6917 |
pobj |
support,without |
R2693 |
T6920 |
T6900 |
prep |
after,survived |
R2694 |
T6921 |
T6922 |
nummod |
48,h |
R2695 |
T6922 |
T6920 |
pobj |
h,after |
R2696 |
T6923 |
T6924 |
advmod |
in,vitro |
R2697 |
T6924 |
T6922 |
advmod |
vitro,h |
R2698 |
T6925 |
T6900 |
cc |
and,survived |
R2699 |
T6926 |
T6927 |
aux |
had,established |
R2700 |
T6927 |
T6900 |
conj |
established,survived |
R2701 |
T6928 |
T6929 |
amod |
long,neurites |
R2702 |
T6929 |
T6927 |
dobj |
neurites,established |
R2703 |
T6930 |
T6928 |
cc |
and,long |
R2704 |
T6931 |
T6932 |
advmod |
highly,branched |
R2705 |
T6932 |
T6928 |
conj |
branched,long |
R2706 |
T6933 |
T6934 |
punct |
(,4D |
R2707 |
T6934 |
T6927 |
parataxis |
4D,established |
R2708 |
T6935 |
T6934 |
compound |
Figure,4D |
R2709 |
T6936 |
T6934 |
punct |
),4D |
R2710 |
T6937 |
T6900 |
punct |
.,survived |
R2711 |
T6939 |
T6940 |
preconj |
Neither,pattern |
R2712 |
T6940 |
T6942 |
nsubj |
pattern,changed |
R2713 |
T6941 |
T6940 |
det |
the,pattern |
R2714 |
T6943 |
T6940 |
prep |
of,pattern |
R2715 |
T6944 |
T6945 |
compound |
neurite,outgrowth |
R2716 |
T6945 |
T6943 |
pobj |
outgrowth,of |
R2717 |
T6946 |
T6940 |
cc |
nor,pattern |
R2718 |
T6947 |
T6948 |
amod |
neuronal,survival |
R2719 |
T6948 |
T6940 |
conj |
survival,pattern |
R2720 |
T6949 |
T6942 |
advmod |
significantly,changed |
R2721 |
T6950 |
T6942 |
prep |
after,changed |
R2722 |
T6951 |
T6950 |
pobj |
application,after |
R2723 |
T6952 |
T6951 |
prep |
of,application |
R2724 |
T6953 |
T6954 |
preconj |
either,NGF |
R2725 |
T6954 |
T6952 |
pobj |
NGF,of |
R2726 |
T6955 |
T6954 |
cc |
or,NGF |
R2727 |
T6956 |
T6954 |
conj |
NT,NGF |
R2728 |
T6957 |
T6956 |
punct |
-,NT |
R2729 |
T6958 |
T6956 |
nummod |
3,NT |
R2730 |
T6959 |
T6960 |
punct |
(,4E |
R2731 |
T6960 |
T6942 |
parataxis |
4E,changed |
R2732 |
T6961 |
T6960 |
compound |
Figure,4E |
R2733 |
T6962 |
T6960 |
cc |
and,4E |
R2734 |
T6963 |
T6960 |
conj |
4F,4E |
R2735 |
T6964 |
T6960 |
punct |
),4E |
R2736 |
T6965 |
T6942 |
punct |
.,changed |
R2737 |
T6967 |
T6968 |
aux |
To,compare |
R2738 |
T6968 |
T6970 |
advcl |
compare,generated |
R2739 |
T6969 |
T6968 |
advmod |
directly,compare |
R2740 |
T6971 |
T6972 |
compound |
neurotrophin,dependence |
R2741 |
T6972 |
T6968 |
dobj |
dependence,compare |
R2742 |
T6973 |
T6972 |
prep |
of,dependence |
R2743 |
T6974 |
T6975 |
compound |
DRG,neurons |
R2744 |
T6975 |
T6973 |
pobj |
neurons,of |
R2745 |
T6976 |
T6975 |
acl |
expressing,neurons |
R2746 |
T6977 |
T6978 |
compound |
EWS,Pea3 |
R2747 |
T6978 |
T6976 |
dobj |
Pea3,expressing |
R2748 |
T6979 |
T6978 |
punct |
-,Pea3 |
R2749 |
T6980 |
T6978 |
prep |
from,Pea3 |
R2750 |
T6981 |
T6982 |
det |
the,locus |
R2751 |
T6982 |
T6980 |
pobj |
locus,from |
R2752 |
T6983 |
T6982 |
compound |
Tau,locus |
R2753 |
T6984 |
T6982 |
prep |
at,locus |
R2754 |
T6985 |
T6986 |
det |
a,time |
R2755 |
T6986 |
T6984 |
pobj |
time,at |
R2756 |
T6987 |
T6986 |
amod |
precocious,time |
R2757 |
T6988 |
T6987 |
cc |
versus,precocious |
R2758 |
T6989 |
T6987 |
conj |
isochronic,precocious |
R2759 |
T6990 |
T6986 |
prep |
of,time |
R2760 |
T6991 |
T6990 |
pobj |
onset,of |
R2761 |
T6992 |
T6970 |
punct |
", ",generated |
R2762 |
T6993 |
T6970 |
nsubj |
we,generated |
R2763 |
T6994 |
T6995 |
det |
a,strain |
R2764 |
T6995 |
T6970 |
dobj |
strain,generated |
R2765 |
T6996 |
T6995 |
prep |
of,strain |
R2766 |
T6997 |
T6996 |
pobj |
mice,of |
R2767 |
T6998 |
T6999 |
prep |
in,expressed |
R2768 |
T6999 |
T6995 |
relcl |
expressed,strain |
R2769 |
T7000 |
T6998 |
pobj |
which,in |
R2770 |
T7001 |
T7002 |
compound |
Cre,recombinase |
R2771 |
T7002 |
T6999 |
nsubjpass |
recombinase,expressed |
R2772 |
T7003 |
T6999 |
auxpass |
is,expressed |
R2773 |
T7004 |
T6999 |
prep |
from,expressed |
R2774 |
T7005 |
T7006 |
det |
the,locus |
R2775 |
T7006 |
T7004 |
pobj |
locus,from |
R2776 |
T7007 |
T7006 |
compound |
PV,locus |
R2777 |
T7008 |
T7009 |
punct |
(,S4 |
R2778 |
T7009 |
T6970 |
parataxis |
S4,generated |
R2779 |
T7010 |
T7009 |
compound |
Figure,S4 |
R2780 |
T7011 |
T7009 |
punct |
),S4 |
R2781 |
T7012 |
T6970 |
punct |
.,generated |
R2782 |
T7014 |
T7015 |
det |
The,expression |
R2783 |
T7015 |
T7016 |
nsubjpass |
expression,restricted |
R2784 |
T7017 |
T7015 |
prep |
of,expression |
R2785 |
T7018 |
T7017 |
pobj |
GFP,of |
R2786 |
T7019 |
T7015 |
prep |
in,expression |
R2787 |
T7020 |
T7021 |
nmod |
TaumGFP,PVCre |
R2788 |
T7021 |
T7019 |
pobj |
PVCre,in |
R2789 |
T7022 |
T7021 |
punct |
/,PVCre |
R2790 |
T7023 |
T7021 |
punct |
+,PVCre |
R2791 |
T7024 |
T7021 |
punct |
/,PVCre |
R2792 |
T7025 |
T7021 |
punct |
+,PVCre |
R2793 |
T7026 |
T7016 |
auxpass |
was,restricted |
R2794 |
T7027 |
T7016 |
prep |
to,restricted |
R2795 |
T7028 |
T7029 |
nmod |
PV,neurons |
R2796 |
T7029 |
T7027 |
pobj |
neurons,to |
R2797 |
T7030 |
T7028 |
punct |
+,PV |
R2798 |
T7031 |
T7029 |
amod |
proprioceptive,neurons |
R2799 |
T7032 |
T7029 |
compound |
DRG,neurons |
R2800 |
T7033 |
T7016 |
cc |
and,restricted |
R2801 |
T7034 |
T7016 |
conj |
mirrored,restricted |
R2802 |
T7035 |
T7036 |
det |
the,onset |
R2803 |
T7036 |
T7034 |
dobj |
onset,mirrored |
R2804 |
T7037 |
T7036 |
prep |
of,onset |
R2805 |
T7038 |
T7037 |
pobj |
expression,of |
R2806 |
T7039 |
T7038 |
prep |
of,expression |
R2807 |
T7040 |
T7039 |
pobj |
PV,of |
R2808 |
T7041 |
T7034 |
prep |
at,mirrored |
R2809 |
T7042 |
T7043 |
advmod |
approximately,E14 |
R2810 |
T7043 |
T7041 |
pobj |
E14,at |
R2811 |
T7044 |
T7045 |
punct |
(,S4 |
R2812 |
T7045 |
T7043 |
parataxis |
S4,E14 |
R2813 |
T7046 |
T7045 |
compound |
Figure,S4 |
R2814 |
T7047 |
T7045 |
punct |
;,S4 |
R2815 |
T7048 |
T7049 |
nsubj |
data,shown |
R2816 |
T7049 |
T7045 |
ccomp |
shown,S4 |
R2817 |
T7050 |
T7049 |
neg |
not,shown |
R2818 |
T7051 |
T7045 |
punct |
),S4 |
R2819 |
T7052 |
T7016 |
punct |
.,restricted |
R2820 |
T7054 |
T7055 |
nsubj |
We,cultured |
R2821 |
T7056 |
T7055 |
advmod |
next,cultured |
R2822 |
T7057 |
T7058 |
nmod |
E14.5,explants |
R2823 |
T7058 |
T7055 |
dobj |
explants,cultured |
R2824 |
T7059 |
T7058 |
amod |
whole,explants |
R2825 |
T7060 |
T7058 |
compound |
DRG,explants |
R2826 |
T7061 |
T7058 |
prep |
from,explants |
R2827 |
T7062 |
T7063 |
nmod |
TauEWS,PVCre |
R2828 |
T7063 |
T7068 |
nmod |
PVCre,mice |
R2829 |
T7064 |
T7063 |
punct |
-,PVCre |
R2830 |
T7065 |
T7063 |
nmod |
Pea3,PVCre |
R2831 |
T7066 |
T7063 |
punct |
/,PVCre |
R2832 |
T7067 |
T7063 |
punct |
+,PVCre |
R2833 |
T7068 |
T7061 |
pobj |
mice,from |
R2834 |
T7069 |
T7063 |
punct |
/,PVCre |
R2835 |
T7070 |
T7063 |
punct |
+,PVCre |
R2836 |
T7071 |
T7063 |
cc |
and,PVCre |
R2837 |
T7072 |
T7073 |
nmod |
TaumGFP,PVCre |
R2838 |
T7073 |
T7063 |
conj |
PVCre,PVCre |
R2839 |
T7074 |
T7073 |
punct |
/,PVCre |
R2840 |
T7075 |
T7073 |
punct |
+,PVCre |
R2841 |
T7076 |
T7073 |
punct |
/,PVCre |
R2842 |
T7077 |
T7073 |
punct |
+,PVCre |
R2843 |
T7078 |
T7055 |
prep |
for,cultured |
R2844 |
T7079 |
T7080 |
nummod |
48,h |
R2845 |
T7080 |
T7078 |
pobj |
h,for |
R2846 |
T7081 |
T7082 |
advmod |
in,vitro |
R2847 |
T7082 |
T7080 |
advmod |
vitro,h |
R2848 |
T7083 |
T7055 |
prep |
in,cultured |
R2849 |
T7084 |
T7085 |
det |
the,presence |
R2850 |
T7085 |
T7083 |
pobj |
presence,in |
R2851 |
T7086 |
T7085 |
cc |
or,presence |
R2852 |
T7087 |
T7085 |
conj |
absence,presence |
R2853 |
T7088 |
T7085 |
prep |
of,presence |
R2854 |
T7089 |
T7088 |
pobj |
NT,of |
R2855 |
T7090 |
T7089 |
punct |
-,NT |
R2856 |
T7091 |
T7089 |
nummod |
3,NT |
R2857 |
T7092 |
T7093 |
punct |
(,Figure |
R2858 |
T7093 |
T7055 |
parataxis |
Figure,cultured |
R2859 |
T7094 |
T7093 |
nummod |
5,Figure |
R2860 |
T7095 |
T7093 |
punct |
),Figure |
R2861 |
T7096 |
T7055 |
punct |
.,cultured |
R2862 |
T7098 |
T7099 |
nsubj |
We,found |
R2863 |
T7100 |
T7101 |
mark |
that,survived |
R2864 |
T7101 |
T7099 |
ccomp |
survived,found |
R2865 |
T7102 |
T7103 |
compound |
DRG,neurons |
R2866 |
T7103 |
T7101 |
nsubj |
neurons,survived |
R2867 |
T7104 |
T7103 |
prep |
from,neurons |
R2868 |
T7105 |
T7106 |
det |
both,genotypes |
R2869 |
T7106 |
T7104 |
pobj |
genotypes,from |
R2870 |
T7107 |
T7101 |
cc |
and,survived |
R2871 |
T7108 |
T7101 |
conj |
extended,survived |
R2872 |
T7109 |
T7108 |
dobj |
neurites,extended |
R2873 |
T7110 |
T7111 |
advmod |
only,in |
R2874 |
T7111 |
T7101 |
prep |
in,survived |
R2875 |
T7112 |
T7113 |
det |
the,presence |
R2876 |
T7113 |
T7111 |
pobj |
presence,in |
R2877 |
T7114 |
T7113 |
prep |
of,presence |
R2878 |
T7115 |
T7114 |
pobj |
NT,of |
R2879 |
T7116 |
T7115 |
punct |
-,NT |
R2880 |
T7117 |
T7115 |
nummod |
3,NT |
R2881 |
T7118 |
T7101 |
punct |
", ",survived |
R2882 |
T7119 |
T7120 |
mark |
whereas,died |
R2883 |
T7120 |
T7101 |
advcl |
died,survived |
R2884 |
T7121 |
T7120 |
nsubj |
they,died |
R2885 |
T7122 |
T7120 |
prep |
in,died |
R2886 |
T7123 |
T7124 |
det |
the,absence |
R2887 |
T7124 |
T7122 |
pobj |
absence,in |
R2888 |
T7125 |
T7124 |
prep |
of,absence |
R2889 |
T7126 |
T7125 |
pobj |
NT,of |
R2890 |
T7127 |
T7126 |
punct |
-,NT |
R2891 |
T7128 |
T7126 |
nummod |
3,NT |
R2892 |
T7129 |
T7130 |
punct |
(,Figure |
R2893 |
T7130 |
T7101 |
parataxis |
Figure,survived |
R2894 |
T7131 |
T7130 |
nummod |
5,Figure |
R2895 |
T7132 |
T7130 |
punct |
),Figure |
R2896 |
T7133 |
T7099 |
punct |
.,found |
R2897 |
T7135 |
T7136 |
advmod |
Together,suggest |
R2898 |
T7137 |
T7136 |
punct |
", ",suggest |
R2899 |
T7138 |
T7139 |
det |
these,findings |
R2900 |
T7139 |
T7136 |
nsubj |
findings,suggest |
R2901 |
T7140 |
T7141 |
mark |
that,is |
R2902 |
T7141 |
T7136 |
ccomp |
is,suggest |
R2903 |
T7142 |
T7143 |
advmod |
only,signaling |
R2904 |
T7143 |
T7141 |
nsubj |
signaling,is |
R2905 |
T7144 |
T7143 |
amod |
precocious,signaling |
R2906 |
T7145 |
T7144 |
cc |
but,precocious |
R2907 |
T7146 |
T7145 |
neg |
not,but |
R2908 |
T7147 |
T7144 |
conj |
isochronic,precocious |
R2909 |
T7148 |
T7143 |
compound |
ETS,signaling |
R2910 |
T7149 |
T7143 |
prep |
in,signaling |
R2911 |
T7150 |
T7151 |
compound |
DRG,neurons |
R2912 |
T7151 |
T7149 |
pobj |
neurons,in |
R2913 |
T7152 |
T7141 |
acomp |
capable,is |
R2914 |
T7153 |
T7152 |
prep |
of,capable |
R2915 |
T7154 |
T7153 |
pcomp |
uncoupling,of |
R2916 |
T7155 |
T7154 |
dobj |
survival,uncoupling |
R2917 |
T7156 |
T7155 |
cc |
and,survival |
R2918 |
T7157 |
T7158 |
compound |
neurite,outgrowth |
R2919 |
T7158 |
T7155 |
conj |
outgrowth,survival |
R2920 |
T7159 |
T7154 |
prep |
from,uncoupling |
R2921 |
T7160 |
T7161 |
det |
a,requirement |
R2922 |
T7161 |
T7159 |
pobj |
requirement,from |
R2923 |
T7162 |
T7161 |
prep |
for,requirement |
R2924 |
T7163 |
T7164 |
compound |
neurotrophin,signaling |
R2925 |
T7164 |
T7162 |
pobj |
signaling,for |
R2926 |
T7165 |
T7166 |
advmod |
normally,observed |
R2927 |
T7166 |
T7161 |
acl |
observed,requirement |
R2928 |
T7167 |
T7166 |
prep |
in,observed |
R2929 |
T7168 |
T7169 |
amod |
wild,type |
R2930 |
T7169 |
T7171 |
compound |
type,DRG |
R2931 |
T7170 |
T7169 |
punct |
-,type |
R2932 |
T7171 |
T7167 |
pobj |
DRG,in |
R2933 |
T7172 |
T7136 |
punct |
.,suggest |
R2934 |
T7174 |
T7175 |
aux |
To,determine |
R2935 |
T7175 |
T7176 |
advcl |
determine,analyzed |
R2936 |
T7177 |
T7178 |
mark |
whether,is |
R2937 |
T7178 |
T7175 |
ccomp |
is,determine |
R2938 |
T7179 |
T7180 |
amod |
neuronal,survival |
R2939 |
T7180 |
T7178 |
nsubj |
survival,is |
R2940 |
T7181 |
T7180 |
prep |
of,survival |
R2941 |
T7182 |
T7183 |
compound |
DRG,neurons |
R2942 |
T7183 |
T7181 |
pobj |
neurons,of |
R2943 |
T7184 |
T7183 |
prep |
from,neurons |
R2944 |
T7185 |
T7186 |
nmod |
TauEWS,Isl1Cre |
R2945 |
T7186 |
T7191 |
nmod |
Isl1Cre,embryos |
R2946 |
T7187 |
T7186 |
punct |
-,Isl1Cre |
R2947 |
T7188 |
T7186 |
nmod |
Pea3,Isl1Cre |
R2948 |
T7189 |
T7186 |
punct |
/,Isl1Cre |
R2949 |
T7190 |
T7186 |
punct |
+,Isl1Cre |
R2950 |
T7191 |
T7184 |
pobj |
embryos,from |
R2951 |
T7192 |
T7186 |
punct |
/,Isl1Cre |
R2952 |
T7193 |
T7186 |
punct |
+,Isl1Cre |
R2953 |
T7194 |
T7180 |
prep |
in,survival |
R2954 |
T7195 |
T7196 |
det |
the,absence |
R2955 |
T7196 |
T7194 |
pobj |
absence,in |
R2956 |
T7197 |
T7196 |
prep |
of,absence |
R2957 |
T7198 |
T7199 |
amod |
neurotrophic,support |
R2958 |
T7199 |
T7197 |
pobj |
support,of |
R2959 |
T7200 |
T7178 |
acomp |
sufficient,is |
R2960 |
T7201 |
T7202 |
aux |
to,explain |
R2961 |
T7202 |
T7200 |
xcomp |
explain,sufficient |
R2962 |
T7203 |
T7204 |
det |
the,outgrowth |
R2963 |
T7204 |
T7202 |
dobj |
outgrowth,explain |
R2964 |
T7205 |
T7204 |
amod |
observed,outgrowth |
R2965 |
T7206 |
T7204 |
amod |
neuronal,outgrowth |
R2966 |
T7207 |
T7176 |
punct |
", ",analyzed |
R2967 |
T7208 |
T7176 |
nsubj |
we,analyzed |
R2968 |
T7209 |
T7176 |
dobj |
DRG,analyzed |
R2969 |
T7210 |
T7209 |
acl |
isolated,DRG |
R2970 |
T7211 |
T7210 |
prep |
from,isolated |
R2971 |
T7212 |
T7213 |
compound |
mice,mutant |
R2972 |
T7213 |
T7211 |
pobj |
mutant,from |
R2973 |
T7214 |
T7176 |
prep |
in,analyzed |
R2974 |
T7215 |
T7216 |
det |
the,Bax |
R2975 |
T7216 |
T7214 |
pobj |
Bax,in |
R2976 |
T7217 |
T7216 |
amod |
proapoptotic,Bax |
R2977 |
T7218 |
T7216 |
compound |
gene,Bax |
R2978 |
T7219 |
T7220 |
punct |
[,27 |
R2979 |
T7220 |
T7176 |
parataxis |
27,analyzed |
R2980 |
T7221 |
T7220 |
punct |
],27 |
R2981 |
T7222 |
T7176 |
punct |
.,analyzed |
R2982 |
T7224 |
T7225 |
advcl |
Consistent,survived |
R2983 |
T7226 |
T7224 |
prep |
with,Consistent |
R2984 |
T7227 |
T7228 |
amod |
previous,results |
R2985 |
T7228 |
T7226 |
pobj |
results,with |
R2986 |
T7229 |
T7225 |
punct |
", ",survived |
R2987 |
T7230 |
T7231 |
nmod |
Bax,neurons |
R2988 |
T7231 |
T7225 |
nsubj |
neurons,survived |
R2989 |
T7232 |
T7230 |
punct |
−,Bax |
R2990 |
T7233 |
T7230 |
punct |
/,Bax |
R2991 |
T7234 |
T7230 |
punct |
−,Bax |
R2992 |
T7235 |
T7231 |
compound |
DRG,neurons |
R2993 |
T7236 |
T7225 |
prep |
without,survived |
R2994 |
T7237 |
T7238 |
amod |
neurotrophic,support |
R2995 |
T7238 |
T7236 |
pobj |
support,without |
R2996 |
T7239 |
T7240 |
punct |
[,28 |
R2997 |
T7240 |
T7225 |
parataxis |
28,survived |
R2998 |
T7241 |
T7240 |
punct |
],28 |
R2999 |
T7242 |
T7225 |
punct |
.,survived |
R3000 |
T7244 |
T7245 |
prep |
In,was |
R3001 |
T7246 |
T7244 |
pobj |
contrast,In |
R3002 |
T7247 |
T7245 |
punct |
", ",was |
R3003 |
T7248 |
T7249 |
compound |
neurite,outgrowth |
R3004 |
T7249 |
T7245 |
nsubj |
outgrowth,was |
R3005 |
T7250 |
T7249 |
prep |
of,outgrowth |
R3006 |
T7251 |
T7252 |
nmod |
Bax,neurons |
R3007 |
T7252 |
T7250 |
pobj |
neurons,of |
R3008 |
T7253 |
T7251 |
punct |
−,Bax |
R3009 |
T7254 |
T7251 |
punct |
/,Bax |
R3010 |
T7255 |
T7251 |
punct |
−,Bax |
R3011 |
T7256 |
T7252 |
compound |
DRG,neurons |
R3012 |
T7257 |
T7258 |
advmod |
significantly,less |
R3013 |
T7258 |
T7245 |
acomp |
less,was |
R3014 |
T7259 |
T7260 |
punct |
(,see |
R3015 |
T7260 |
T7258 |
parataxis |
see,less |
R3016 |
T7261 |
T7262 |
compound |
Figure,4G |
R3017 |
T7262 |
T7260 |
dobj |
4G,see |
R3018 |
T7263 |
T7260 |
punct |
),see |
R3019 |
T7264 |
T7258 |
prep |
than,less |
R3020 |
T7265 |
T7264 |
pobj |
that,than |
R3021 |
T7266 |
T7265 |
prep |
of,that |
R3022 |
T7267 |
T7268 |
preconj |
either,DRG |
R3023 |
T7268 |
T7266 |
pobj |
DRG,of |
R3024 |
T7269 |
T7268 |
prep |
from,DRG |
R3025 |
T7270 |
T7271 |
nmod |
TauEWS,Isl1Cre |
R3026 |
T7271 |
T7276 |
nmod |
Isl1Cre,embryos |
R3027 |
T7272 |
T7271 |
punct |
-,Isl1Cre |
R3028 |
T7273 |
T7271 |
nmod |
Pea3,Isl1Cre |
R3029 |
T7274 |
T7271 |
punct |
/,Isl1Cre |
R3030 |
T7275 |
T7271 |
punct |
+,Isl1Cre |
R3031 |
T7276 |
T7269 |
pobj |
embryos,from |
R3032 |
T7277 |
T7271 |
punct |
/,Isl1Cre |
R3033 |
T7278 |
T7271 |
punct |
+,Isl1Cre |
R3034 |
T7279 |
T7268 |
acl |
cultured,DRG |
R3035 |
T7280 |
T7279 |
prep |
in,cultured |
R3036 |
T7281 |
T7282 |
det |
the,absence |
R3037 |
T7282 |
T7280 |
pobj |
absence,in |
R3038 |
T7283 |
T7282 |
prep |
of,absence |
R3039 |
T7284 |
T7285 |
amod |
neurotrophic,support |
R3040 |
T7285 |
T7283 |
pobj |
support,of |
R3041 |
T7286 |
T7287 |
punct |
(,see |
R3042 |
T7287 |
T7279 |
parataxis |
see,cultured |
R3043 |
T7288 |
T7289 |
compound |
Figure,4D |
R3044 |
T7289 |
T7287 |
dobj |
4D,see |
R3045 |
T7290 |
T7287 |
punct |
),see |
R3046 |
T7291 |
T7268 |
cc |
or,DRG |
R3047 |
T7292 |
T7293 |
nmod |
Bax,neurons |
R3048 |
T7293 |
T7268 |
conj |
neurons,DRG |
R3049 |
T7294 |
T7292 |
punct |
−,Bax |
R3050 |
T7295 |
T7292 |
punct |
/,Bax |
R3051 |
T7296 |
T7292 |
punct |
−,Bax |
R3052 |
T7297 |
T7293 |
compound |
DRG,neurons |
R3053 |
T7298 |
T7293 |
acl |
cultured,neurons |
R3054 |
T7299 |
T7298 |
prep |
in,cultured |
R3055 |
T7300 |
T7301 |
det |
the,presence |
R3056 |
T7301 |
T7299 |
pobj |
presence,in |
R3057 |
T7302 |
T7301 |
prep |
of,presence |
R3058 |
T7303 |
T7304 |
amod |
neurotrophic,support |
R3059 |
T7304 |
T7302 |
pobj |
support,of |
R3060 |
T7305 |
T7306 |
punct |
(,see |
R3061 |
T7306 |
T7298 |
parataxis |
see,cultured |
R3062 |
T7307 |
T7308 |
compound |
Figure,4H |
R3063 |
T7308 |
T7306 |
dobj |
4H,see |
R3064 |
T7309 |
T7308 |
cc |
and,4H |
R3065 |
T7310 |
T7308 |
conj |
4I,4H |
R3066 |
T7311 |
T7306 |
punct |
),see |
R3067 |
T7312 |
T7245 |
punct |
.,was |
R3068 |
T7314 |
T7315 |
det |
These,findings |
R3069 |
T7315 |
T7316 |
nsubj |
findings,suggest |
R3070 |
T7317 |
T7318 |
mark |
that,promotes |
R3071 |
T7318 |
T7316 |
ccomp |
promotes,suggest |
R3072 |
T7319 |
T7318 |
prep |
in,promotes |
R3073 |
T7320 |
T7319 |
pobj |
addition,in |
R3074 |
T7321 |
T7320 |
prep |
to,addition |
R3075 |
T7322 |
T7321 |
pcomp |
mediating,to |
R3076 |
T7323 |
T7324 |
npadvmod |
neurotrophin,independent |
R3077 |
T7324 |
T7326 |
amod |
independent,survival |
R3078 |
T7325 |
T7324 |
punct |
-,independent |
R3079 |
T7326 |
T7322 |
dobj |
survival,mediating |
R3080 |
T7327 |
T7326 |
amod |
neuronal,survival |
R3081 |
T7328 |
T7318 |
punct |
", ",promotes |
R3082 |
T7329 |
T7318 |
nsubj |
expression,promotes |
R3083 |
T7330 |
T7329 |
prep |
of,expression |
R3084 |
T7331 |
T7332 |
compound |
EWS,Pea3 |
R3085 |
T7332 |
T7330 |
pobj |
Pea3,of |
R3086 |
T7333 |
T7332 |
punct |
-,Pea3 |
R3087 |
T7334 |
T7329 |
prep |
in,expression |
R3088 |
T7335 |
T7336 |
amod |
early,neurons |
R3089 |
T7336 |
T7334 |
pobj |
neurons,in |
R3090 |
T7337 |
T7336 |
amod |
post-mitotic,neurons |
R3091 |
T7338 |
T7318 |
advmod |
also,promotes |
R3092 |
T7339 |
T7340 |
compound |
neurite,outgrowth |
R3093 |
T7340 |
T7318 |
dobj |
outgrowth,promotes |
R3094 |
T7341 |
T7318 |
prep |
in,promotes |
R3095 |
T7342 |
T7343 |
det |
a,manner |
R3096 |
T7343 |
T7341 |
pobj |
manner,in |
R3097 |
T7344 |
T7345 |
npadvmod |
neurotrophin,independent |
R3098 |
T7345 |
T7343 |
amod |
independent,manner |
R3099 |
T7346 |
T7345 |
punct |
-,independent |
R3100 |
T7347 |
T7316 |
punct |
.,suggest |
R3101 |
T7349 |
T7350 |
aux |
To,begin |
R3102 |
T7350 |
T7351 |
advcl |
begin,assayed |
R3103 |
T7352 |
T7353 |
aux |
to,assess |
R3104 |
T7353 |
T7350 |
xcomp |
assess,begin |
R3105 |
T7354 |
T7355 |
prep |
at,become |
R3106 |
T7355 |
T7353 |
ccomp |
become,assess |
R3107 |
T7356 |
T7357 |
det |
which,step |
R3108 |
T7357 |
T7354 |
pcomp |
step,at |
R3109 |
T7358 |
T7357 |
prep |
of,step |
R3110 |
T7359 |
T7360 |
det |
the,cascade |
R3111 |
T7360 |
T7358 |
pobj |
cascade,of |
R3112 |
T7361 |
T7360 |
compound |
neurotrophin,cascade |
R3113 |
T7362 |
T7360 |
compound |
signaling,cascade |
R3114 |
T7363 |
T7364 |
compound |
DRG,neurons |
R3115 |
T7364 |
T7355 |
nsubj |
neurons,become |
R3116 |
T7365 |
T7364 |
prep |
from,neurons |
R3117 |
T7366 |
T7367 |
nmod |
TauEWS,Isl1Cre |
R3118 |
T7367 |
T7372 |
nmod |
Isl1Cre,embryos |
R3119 |
T7368 |
T7367 |
punct |
-,Isl1Cre |
R3120 |
T7369 |
T7367 |
nmod |
Pea3,Isl1Cre |
R3121 |
T7370 |
T7367 |
punct |
/,Isl1Cre |
R3122 |
T7371 |
T7367 |
punct |
+,Isl1Cre |
R3123 |
T7372 |
T7365 |
pobj |
embryos,from |
R3124 |
T7373 |
T7367 |
punct |
/,Isl1Cre |
R3125 |
T7374 |
T7367 |
punct |
+,Isl1Cre |
R3126 |
T7375 |
T7355 |
acomp |
unresponsive,become |
R3127 |
T7376 |
T7375 |
prep |
to,unresponsive |
R3128 |
T7377 |
T7378 |
det |
the,addition |
R3129 |
T7378 |
T7376 |
pobj |
addition,to |
R3130 |
T7379 |
T7378 |
prep |
of,addition |
R3131 |
T7380 |
T7379 |
pobj |
neurotrophins,of |
R3132 |
T7381 |
T7351 |
punct |
", ",assayed |
R3133 |
T7382 |
T7351 |
nsubj |
we,assayed |
R3134 |
T7383 |
T7384 |
det |
the,expression |
R3135 |
T7384 |
T7351 |
dobj |
expression,assayed |
R3136 |
T7385 |
T7384 |
prep |
of,expression |
R3137 |
T7386 |
T7387 |
compound |
neurotrophin,receptors |
R3138 |
T7387 |
T7385 |
pobj |
receptors,of |
R3139 |
T7388 |
T7351 |
prep |
in,assayed |
R3140 |
T7389 |
T7390 |
nmod |
TauEWS,Isl1Cre |
R3141 |
T7390 |
T7395 |
nmod |
Isl1Cre,embryos |
R3142 |
T7391 |
T7390 |
punct |
-,Isl1Cre |
R3143 |
T7392 |
T7390 |
nmod |
Pea3,Isl1Cre |
R3144 |
T7393 |
T7390 |
punct |
/,Isl1Cre |
R3145 |
T7394 |
T7390 |
punct |
+,Isl1Cre |
R3146 |
T7395 |
T7388 |
pobj |
embryos,in |
R3147 |
T7396 |
T7390 |
punct |
/,Isl1Cre |
R3148 |
T7397 |
T7390 |
punct |
+,Isl1Cre |
R3149 |
T7398 |
T7399 |
punct |
(,Figure |
R3150 |
T7399 |
T7351 |
parataxis |
Figure,assayed |
R3151 |
T7400 |
T7399 |
nummod |
6,Figure |
R3152 |
T7401 |
T7399 |
punct |
),Figure |
R3153 |
T7402 |
T7351 |
punct |
.,assayed |
R3154 |
T7404 |
T7405 |
mark |
Whereas,marks |
R3155 |
T7405 |
T7417 |
advcl |
marks,showed |
R3156 |
T7406 |
T7405 |
nsubj |
expression,marks |
R3157 |
T7407 |
T7406 |
prep |
of,expression |
R3158 |
T7408 |
T7409 |
det |
the,receptors |
R3159 |
T7409 |
T7407 |
pobj |
receptors,of |
R3160 |
T7410 |
T7409 |
compound |
neurotrophin,receptors |
R3161 |
T7411 |
T7409 |
appos |
TrkA,receptors |
R3162 |
T7412 |
T7411 |
punct |
", ",TrkA |
R3163 |
T7413 |
T7411 |
conj |
TrkB,TrkA |
R3164 |
T7414 |
T7413 |
punct |
", ",TrkB |
R3165 |
T7415 |
T7413 |
cc |
and,TrkB |
R3166 |
T7416 |
T7413 |
conj |
TrkC,TrkB |
R3167 |
T7418 |
T7405 |
dobj |
afferents,marks |
R3168 |
T7419 |
T7418 |
prep |
of,afferents |
R3169 |
T7420 |
T7421 |
amod |
distinct,modalities |
R3170 |
T7421 |
T7419 |
pobj |
modalities,of |
R3171 |
T7422 |
T7421 |
amod |
sensory,modalities |
R3172 |
T7423 |
T7405 |
prep |
in,marks |
R3173 |
T7424 |
T7423 |
pobj |
DRG,in |
R3174 |
T7425 |
T7424 |
prep |
of,DRG |
R3175 |
T7426 |
T7427 |
amod |
wild,type |
R3176 |
T7427 |
T7429 |
compound |
type,embryos |
R3177 |
T7428 |
T7427 |
punct |
-,type |
R3178 |
T7429 |
T7425 |
pobj |
embryos,of |
R3179 |
T7430 |
T7431 |
punct |
(,6A |
R3180 |
T7431 |
T7405 |
parataxis |
6A,marks |
R3181 |
T7432 |
T7431 |
compound |
Figure,6A |
R3182 |
T7433 |
T7434 |
punct |
–,6C |
R3183 |
T7434 |
T7431 |
prep |
6C,6A |
R3184 |
T7435 |
T7431 |
punct |
),6A |
R3185 |
T7436 |
T7437 |
punct |
[,29 |
R3186 |
T7437 |
T7405 |
parataxis |
29,marks |
R3187 |
T7438 |
T7437 |
dep |
4,29 |
R3188 |
T7439 |
T7437 |
punct |
",",29 |
R3189 |
T7440 |
T7437 |
punct |
],29 |
R3190 |
T7441 |
T7417 |
punct |
", ",showed |
R3191 |
T7442 |
T7443 |
nmod |
TauEWS,Isl1Cre |
R3192 |
T7443 |
T7448 |
nmod |
Isl1Cre,embryos |
R3193 |
T7444 |
T7443 |
punct |
-,Isl1Cre |
R3194 |
T7445 |
T7443 |
nmod |
Pea3,Isl1Cre |
R3195 |
T7446 |
T7443 |
punct |
/,Isl1Cre |
R3196 |
T7447 |
T7443 |
punct |
+,Isl1Cre |
R3197 |
T7448 |
T7417 |
nsubj |
embryos,showed |
R3198 |
T7449 |
T7443 |
punct |
/,Isl1Cre |
R3199 |
T7450 |
T7443 |
punct |
+,Isl1Cre |
R3200 |
T7451 |
T7452 |
amod |
complete,absence |
R3201 |
T7452 |
T7417 |
dobj |
absence,showed |
R3202 |
T7453 |
T7452 |
prep |
of,absence |
R3203 |
T7454 |
T7453 |
pobj |
expression,of |
R3204 |
T7455 |
T7454 |
prep |
of,expression |
R3205 |
T7456 |
T7455 |
pobj |
TrkA,of |
R3206 |
T7457 |
T7456 |
punct |
", ",TrkA |
R3207 |
T7458 |
T7456 |
conj |
TrkB,TrkA |
R3208 |
T7459 |
T7458 |
punct |
", ",TrkB |
R3209 |
T7460 |
T7458 |
cc |
and,TrkB |
R3210 |
T7461 |
T7458 |
conj |
TrkC,TrkB |
R3211 |
T7462 |
T7454 |
prep |
in,expression |
R3212 |
T7463 |
T7464 |
compound |
DRG,neurons |
R3213 |
T7464 |
T7462 |
pobj |
neurons,in |
R3214 |
T7465 |
T7417 |
prep |
at,showed |
R3215 |
T7466 |
T7465 |
pobj |
E16.5,at |
R3216 |
T7467 |
T7468 |
punct |
(,6G |
R3217 |
T7468 |
T7417 |
parataxis |
6G,showed |
R3218 |
T7469 |
T7468 |
compound |
Figure,6G |
R3219 |
T7470 |
T7471 |
punct |
–,6I |
R3220 |
T7471 |
T7468 |
prep |
6I,6G |
R3221 |
T7472 |
T7468 |
punct |
),6G |
R3222 |
T7473 |
T7417 |
punct |
.,showed |
R3223 |
T7475 |
T7476 |
det |
This,absence |
R3224 |
T7476 |
T7477 |
nsubj |
absence,provides |
R3225 |
T7478 |
T7476 |
prep |
of,absence |
R3226 |
T7479 |
T7480 |
compound |
Trk,expression |
R3227 |
T7480 |
T7478 |
pobj |
expression,of |
R3228 |
T7481 |
T7480 |
compound |
receptor,expression |
R3229 |
T7482 |
T7476 |
prep |
in,absence |
R3230 |
T7483 |
T7482 |
pobj |
DRG,in |
R3231 |
T7484 |
T7483 |
prep |
of,DRG |
R3232 |
T7485 |
T7486 |
nmod |
TauEWS,Isl1Cre |
R3233 |
T7486 |
T7491 |
nmod |
Isl1Cre,embryos |
R3234 |
T7487 |
T7486 |
punct |
-,Isl1Cre |
R3235 |
T7488 |
T7486 |
nmod |
Pea3,Isl1Cre |
R3236 |
T7489 |
T7486 |
punct |
/,Isl1Cre |
R3237 |
T7490 |
T7486 |
punct |
+,Isl1Cre |
R3238 |
T7491 |
T7484 |
pobj |
embryos,of |
R3239 |
T7492 |
T7486 |
punct |
/,Isl1Cre |
R3240 |
T7493 |
T7486 |
punct |
+,Isl1Cre |
R3241 |
T7494 |
T7495 |
det |
a,explanation |
R3242 |
T7495 |
T7477 |
dobj |
explanation,provides |
R3243 |
T7496 |
T7495 |
amod |
likely,explanation |
R3244 |
T7497 |
T7495 |
prep |
for,explanation |
R3245 |
T7498 |
T7499 |
det |
the,lack |
R3246 |
T7499 |
T7497 |
pobj |
lack,for |
R3247 |
T7500 |
T7499 |
prep |
of,lack |
R3248 |
T7501 |
T7500 |
pobj |
responsiveness,of |
R3249 |
T7502 |
T7501 |
prep |
of,responsiveness |
R3250 |
T7503 |
T7504 |
det |
these,neurons |
R3251 |
T7504 |
T7502 |
pobj |
neurons,of |
R3252 |
T7505 |
T7501 |
prep |
to,responsiveness |
R3253 |
T7506 |
T7507 |
det |
the,addition |
R3254 |
T7507 |
T7505 |
pobj |
addition,to |
R3255 |
T7508 |
T7507 |
prep |
of,addition |
R3256 |
T7509 |
T7510 |
amod |
neurotrophic,factors |
R3257 |
T7510 |
T7508 |
pobj |
factors,of |
R3258 |
T7511 |
T7477 |
punct |
.,provides |
R3259 |
T7513 |
T7514 |
nsubj |
We,assayed |
R3260 |
T7515 |
T7514 |
advmod |
next,assayed |
R3261 |
T7516 |
T7517 |
mark |
whether,had |
R3262 |
T7517 |
T7514 |
ccomp |
had,assayed |
R3263 |
T7518 |
T7519 |
det |
the,absence |
R3264 |
T7519 |
T7517 |
nsubj |
absence,had |
R3265 |
T7520 |
T7519 |
amod |
complete,absence |
R3266 |
T7521 |
T7519 |
prep |
of,absence |
R3267 |
T7522 |
T7523 |
compound |
Trk,expression |
R3268 |
T7523 |
T7521 |
pobj |
expression,of |
R3269 |
T7524 |
T7523 |
compound |
receptor,expression |
R3270 |
T7525 |
T7519 |
prep |
in,absence |
R3271 |
T7526 |
T7527 |
nmod |
TauEWS,Isl1Cre |
R3272 |
T7527 |
T7532 |
nmod |
Isl1Cre,embryos |
R3273 |
T7528 |
T7527 |
punct |
-,Isl1Cre |
R3274 |
T7529 |
T7527 |
nmod |
Pea3,Isl1Cre |
R3275 |
T7530 |
T7527 |
punct |
/,Isl1Cre |
R3276 |
T7531 |
T7527 |
punct |
+,Isl1Cre |
R3277 |
T7532 |
T7525 |
pobj |
embryos,in |
R3278 |
T7533 |
T7527 |
punct |
/,Isl1Cre |
R3279 |
T7534 |
T7527 |
punct |
+,Isl1Cre |
R3280 |
T7535 |
T7536 |
det |
an,influence |
R3281 |
T7536 |
T7517 |
dobj |
influence,had |
R3282 |
T7537 |
T7536 |
prep |
on,influence |
R3283 |
T7538 |
T7539 |
advmod |
naturally,occurring |
R3284 |
T7539 |
T7540 |
amod |
occurring,death |
R3285 |
T7540 |
T7537 |
pobj |
death,on |
R3286 |
T7541 |
T7540 |
compound |
cell,death |
R3287 |
T7542 |
T7543 |
advmod |
in,vivo |
R3288 |
T7543 |
T7540 |
advmod |
vivo,death |
R3289 |
T7544 |
T7514 |
advcl |
using,assayed |
R3290 |
T7545 |
T7544 |
dobj |
TUNEL,using |
R3291 |
T7546 |
T7544 |
prep |
on,using |
R3292 |
T7547 |
T7548 |
compound |
DRG,sections |
R3293 |
T7548 |
T7546 |
pobj |
sections,on |
R3294 |
T7549 |
T7514 |
punct |
.,assayed |
R3295 |
T7551 |
T7552 |
advmod |
Surprisingly,found |
R3296 |
T7553 |
T7552 |
punct |
", ",found |
R3297 |
T7554 |
T7552 |
nsubj |
we,found |
R3298 |
T7555 |
T7556 |
mark |
that,decreased |
R3299 |
T7556 |
T7552 |
ccomp |
decreased,found |
R3300 |
T7557 |
T7556 |
nsubjpass |
apoptosis,decreased |
R3301 |
T7558 |
T7556 |
auxpass |
was,decreased |
R3302 |
T7559 |
T7556 |
prep |
by,decreased |
R3303 |
T7560 |
T7561 |
advmod |
approximately,50 |
R3304 |
T7561 |
T7562 |
nummod |
50,% |
R3305 |
T7562 |
T7559 |
pobj |
%,by |
R3306 |
T7563 |
T7564 |
punct |
(,average |
R3307 |
T7564 |
T7562 |
parataxis |
average,% |
R3308 |
T7565 |
T7566 |
nsubj |
n,embryos |
R3309 |
T7566 |
T7564 |
ccomp |
embryos,average |
R3310 |
T7567 |
T7566 |
punct |
=,embryos |
R3311 |
T7568 |
T7566 |
nummod |
3,embryos |
R3312 |
T7569 |
T7564 |
punct |
", ",average |
R3313 |
T7570 |
T7564 |
prep |
of,average |
R3314 |
T7571 |
T7572 |
punct |
>,50 |
R3315 |
T7572 |
T7573 |
nummod |
50,sections |
R3316 |
T7573 |
T7570 |
pobj |
sections,of |
R3317 |
T7574 |
T7564 |
punct |
),average |
R3318 |
T7575 |
T7556 |
prep |
in,decreased |
R3319 |
T7576 |
T7575 |
pobj |
DRG,in |
R3320 |
T7577 |
T7576 |
prep |
of,DRG |
R3321 |
T7578 |
T7579 |
nmod |
TauEWS,Isl1Cre |
R3322 |
T7579 |
T7584 |
nmod |
Isl1Cre,embryos |
R3323 |
T7580 |
T7579 |
punct |
-,Isl1Cre |
R3324 |
T7581 |
T7579 |
nmod |
Pea3,Isl1Cre |
R3325 |
T7582 |
T7579 |
punct |
/,Isl1Cre |
R3326 |
T7583 |
T7579 |
punct |
+,Isl1Cre |
R3327 |
T7584 |
T7577 |
pobj |
embryos,of |
R3328 |
T7585 |
T7579 |
punct |
/,Isl1Cre |
R3329 |
T7586 |
T7579 |
punct |
+,Isl1Cre |
R3330 |
T7587 |
T7556 |
prep |
in,decreased |
R3331 |
T7588 |
T7587 |
pobj |
comparison,in |
R3332 |
T7589 |
T7588 |
prep |
to,comparison |
R3333 |
T7590 |
T7591 |
amod |
wild,type |
R3334 |
T7591 |
T7589 |
pobj |
type,to |
R3335 |
T7592 |
T7591 |
punct |
-,type |
R3336 |
T7593 |
T7594 |
punct |
(,6D |
R3337 |
T7594 |
T7556 |
parataxis |
6D,decreased |
R3338 |
T7595 |
T7594 |
compound |
Figure,6D |
R3339 |
T7596 |
T7594 |
punct |
", ",6D |
R3340 |
T7597 |
T7594 |
conj |
6J,6D |
R3341 |
T7598 |
T7597 |
punct |
", ",6J |
R3342 |
T7599 |
T7597 |
cc |
and,6J |
R3343 |
T7600 |
T7597 |
conj |
6M,6J |
R3344 |
T7601 |
T7594 |
punct |
),6D |
R3345 |
T7602 |
T7552 |
punct |
.,found |
R3346 |
T7604 |
T7605 |
amod |
Quantitative,analysis |
R3347 |
T7605 |
T7606 |
nsubj |
analysis,revealed |
R3348 |
T7607 |
T7605 |
prep |
of,analysis |
R3349 |
T7608 |
T7609 |
det |
the,number |
R3350 |
T7609 |
T7607 |
pobj |
number,of |
R3351 |
T7610 |
T7609 |
prep |
of,number |
R3352 |
T7611 |
T7610 |
pobj |
neurons,of |
R3353 |
T7612 |
T7611 |
prep |
in,neurons |
R3354 |
T7613 |
T7614 |
compound |
lumbar,DRG |
R3355 |
T7614 |
T7612 |
pobj |
DRG,in |
R3356 |
T7615 |
T7614 |
prep |
of,DRG |
R3357 |
T7616 |
T7617 |
nmod |
TauEWS,Isl1Cre |
R3358 |
T7617 |
T7622 |
nmod |
Isl1Cre,embryos |
R3359 |
T7618 |
T7617 |
punct |
-,Isl1Cre |
R3360 |
T7619 |
T7617 |
nmod |
Pea3,Isl1Cre |
R3361 |
T7620 |
T7617 |
punct |
/,Isl1Cre |
R3362 |
T7621 |
T7617 |
punct |
+,Isl1Cre |
R3363 |
T7622 |
T7615 |
pobj |
embryos,of |
R3364 |
T7623 |
T7617 |
punct |
/,Isl1Cre |
R3365 |
T7624 |
T7617 |
punct |
+,Isl1Cre |
R3366 |
T7625 |
T7626 |
det |
a,increase |
R3367 |
T7626 |
T7606 |
dobj |
increase,revealed |
R3368 |
T7627 |
T7626 |
amod |
significant,increase |
R3369 |
T7628 |
T7626 |
prep |
to,increase |
R3370 |
T7629 |
T7630 |
advmod |
approximately,170 |
R3371 |
T7630 |
T7631 |
nummod |
170,% |
R3372 |
T7631 |
T7628 |
pobj |
%,to |
R3373 |
T7632 |
T7631 |
prep |
of,% |
R3374 |
T7633 |
T7634 |
amod |
wild,type |
R3375 |
T7634 |
T7636 |
compound |
type,levels |
R3376 |
T7635 |
T7634 |
punct |
-,type |
R3377 |
T7636 |
T7632 |
pobj |
levels,of |
R3378 |
T7637 |
T7638 |
punct |
(,6E |
R3379 |
T7638 |
T7606 |
parataxis |
6E,revealed |
R3380 |
T7639 |
T7638 |
compound |
Figure,6E |
R3381 |
T7640 |
T7638 |
punct |
", ",6E |
R3382 |
T7641 |
T7638 |
conj |
6K,6E |
R3383 |
T7642 |
T7641 |
punct |
", ",6K |
R3384 |
T7643 |
T7641 |
cc |
and,6K |
R3385 |
T7644 |
T7641 |
conj |
6N,6K |
R3386 |
T7645 |
T7638 |
punct |
),6E |
R3387 |
T7646 |
T7606 |
punct |
.,revealed |
R3388 |
T7648 |
T7649 |
advmod |
Moreover,ruled |
R3389 |
T7650 |
T7649 |
punct |
", ",ruled |
R3390 |
T7651 |
T7652 |
compound |
BrdU,experiments |
R3391 |
T7652 |
T7649 |
nsubj |
experiments,ruled |
R3392 |
T7653 |
T7654 |
compound |
pulse,chase |
R3393 |
T7654 |
T7652 |
compound |
chase,experiments |
R3394 |
T7655 |
T7654 |
punct |
-,chase |
R3395 |
T7656 |
T7649 |
prt |
out,ruled |
R3396 |
T7657 |
T7658 |
det |
the,possibility |
R3397 |
T7658 |
T7649 |
dobj |
possibility,ruled |
R3398 |
T7659 |
T7660 |
mark |
that,reenter |
R3399 |
T7660 |
T7658 |
acl |
reenter,possibility |
R3400 |
T7661 |
T7662 |
compound |
DRG,neurons |
R3401 |
T7662 |
T7660 |
nsubj |
neurons,reenter |
R3402 |
T7663 |
T7662 |
prep |
in,neurons |
R3403 |
T7664 |
T7665 |
nmod |
TauEWS,Isl1Cre |
R3404 |
T7665 |
T7670 |
nmod |
Isl1Cre,embryos |
R3405 |
T7666 |
T7665 |
punct |
-,Isl1Cre |
R3406 |
T7667 |
T7665 |
nmod |
Pea3,Isl1Cre |
R3407 |
T7668 |
T7665 |
punct |
/,Isl1Cre |
R3408 |
T7669 |
T7665 |
punct |
+,Isl1Cre |
R3409 |
T7670 |
T7663 |
pobj |
embryos,in |
R3410 |
T7671 |
T7665 |
punct |
/,Isl1Cre |
R3411 |
T7672 |
T7665 |
punct |
+,Isl1Cre |
R3412 |
T7673 |
T7674 |
det |
the,cycle |
R3413 |
T7674 |
T7660 |
dobj |
cycle,reenter |
R3414 |
T7675 |
T7674 |
compound |
cell,cycle |
R3415 |
T7676 |
T7677 |
punct |
(,6F |
R3416 |
T7677 |
T7660 |
parataxis |
6F,reenter |
R3417 |
T7678 |
T7679 |
det |
no,cells |
R3418 |
T7679 |
T7677 |
dep |
cells,6F |
R3419 |
T7680 |
T7681 |
nmod |
BrdU,LacZ |
R3420 |
T7681 |
T7679 |
nmod |
LacZ,cells |
R3421 |
T7682 |
T7681 |
punct |
+,LacZ |
R3422 |
T7683 |
T7681 |
punct |
/,LacZ |
R3423 |
T7684 |
T7681 |
punct |
+,LacZ |
R3424 |
T7685 |
T7677 |
punct |
", ",6F |
R3425 |
T7686 |
T7687 |
nsubj |
n,embryos |
R3426 |
T7687 |
T7677 |
ccomp |
embryos,6F |
R3427 |
T7688 |
T7687 |
punct |
=,embryos |
R3428 |
T7689 |
T7687 |
nummod |
3,embryos |
R3429 |
T7690 |
T7677 |
punct |
", ",6F |
R3430 |
T7691 |
T7677 |
dep |
analysis,6F |
R3431 |
T7692 |
T7691 |
prep |
of,analysis |
R3432 |
T7693 |
T7694 |
punct |
>,50 |
R3433 |
T7694 |
T7695 |
nummod |
50,sections |
R3434 |
T7695 |
T7692 |
pobj |
sections,of |
R3435 |
T7751 |
T7748 |
conj |
support,receptors |
R3436 |
T7696 |
T7695 |
advmod |
each,sections |
R3437 |
T7752 |
T7705 |
punct |
.,suggest |
R3438 |
T7697 |
T7677 |
punct |
;,6F |
R3439 |
T7754 |
T7755 |
nsubj |
We,analyzed |
R3440 |
T7698 |
T7677 |
compound |
Figure,6F |
R3441 |
T7756 |
T7755 |
advmod |
next,analyzed |
R3442 |
T7757 |
T7758 |
mark |
whether,detected |
R3443 |
T7699 |
T7677 |
cc |
and,6F |
R3444 |
T7758 |
T7755 |
ccomp |
detected,analyzed |
R3445 |
T7759 |
T7758 |
nsubjpass |
changes,detected |
R3446 |
T7760 |
T7759 |
prep |
in,changes |
R3447 |
T7700 |
T7677 |
conj |
6L,6F |
R3448 |
T7761 |
T7762 |
det |
the,expression |
R3449 |
T7762 |
T7760 |
pobj |
expression,in |
R3450 |
T7763 |
T7762 |
prep |
of,expression |
R3451 |
T7701 |
T7677 |
punct |
),6F |
R3452 |
T7764 |
T7763 |
pobj |
proteins,of |
R3453 |
T7765 |
T7764 |
acl |
known,proteins |
R3454 |
T7702 |
T7649 |
punct |
.,ruled |
R3455 |
T7766 |
T7767 |
aux |
to,involved |
R3456 |
T7767 |
T7765 |
xcomp |
involved,known |
R3457 |
T7768 |
T7767 |
auxpass |
be,involved |
R3458 |
T7704 |
T7705 |
advmod |
Together,suggest |
R3459 |
T7769 |
T7767 |
prep |
in,involved |
R3460 |
T7770 |
T7771 |
det |
the,regulation |
R3461 |
T7771 |
T7769 |
pobj |
regulation,in |
R3462 |
T7772 |
T7771 |
prep |
of,regulation |
R3463 |
T7773 |
T7774 |
amod |
neuronal,survival |
R3464 |
T7706 |
T7704 |
prep |
with,Together |
R3465 |
T7774 |
T7772 |
pobj |
survival,of |
R3466 |
T7775 |
T7774 |
cc |
or,survival |
R3467 |
T7776 |
T7777 |
compound |
cell,death |
R3468 |
T7707 |
T7708 |
det |
the,experiments |
R3469 |
T7777 |
T7774 |
conj |
death,survival |
R3470 |
T7778 |
T7758 |
aux |
could,detected |
R3471 |
T7779 |
T7758 |
auxpass |
be,detected |
R3472 |
T7708 |
T7706 |
pobj |
experiments,with |
R3473 |
T7780 |
T7758 |
prep |
in,detected |
R3474 |
T7781 |
T7780 |
pobj |
DRG,in |
R3475 |
T7782 |
T7781 |
prep |
of,DRG |
R3476 |
T7709 |
T7710 |
advmod |
in,vitro |
R3477 |
T7783 |
T7784 |
nmod |
TauEWS,Isl1Cre |
R3478 |
T7784 |
T7789 |
nmod |
Isl1Cre,embryos |
R3479 |
T7710 |
T7708 |
advmod |
vitro,experiments |
R3480 |
T7785 |
T7784 |
punct |
-,Isl1Cre |
R3481 |
T7786 |
T7784 |
nmod |
Pea3,Isl1Cre |
R3482 |
T7787 |
T7784 |
punct |
/,Isl1Cre |
R3483 |
T7711 |
T7708 |
compound |
culture,experiments |
R3484 |
T7788 |
T7784 |
punct |
+,Isl1Cre |
R3485 |
T7789 |
T7782 |
pobj |
embryos,of |
R3486 |
T7790 |
T7784 |
punct |
/,Isl1Cre |
R3487 |
T7791 |
T7784 |
punct |
+,Isl1Cre |
R3488 |
T7792 |
T7755 |
punct |
.,analyzed |
R3489 |
T7712 |
T7705 |
punct |
", ",suggest |
R3490 |
T7794 |
T7795 |
nsubj |
We,found |
R3491 |
T7796 |
T7797 |
det |
no,changes |
R3492 |
T7713 |
T7714 |
det |
these,findings |
R3493 |
T7797 |
T7795 |
dobj |
changes,found |
R3494 |
T7798 |
T7797 |
amod |
significant,changes |
R3495 |
T7799 |
T7797 |
amod |
quantitative,changes |
R3496 |
T7714 |
T7705 |
nsubj |
findings,suggest |
R3497 |
T7800 |
T7797 |
prep |
in,changes |
R3498 |
T7801 |
T7802 |
det |
the,level |
R3499 |
T7715 |
T7716 |
mark |
that,remain |
R3500 |
T7716 |
T7705 |
ccomp |
remain,suggest |
R3501 |
T7802 |
T7800 |
pobj |
level,in |
R3502 |
T7803 |
T7802 |
prep |
of,level |
R3503 |
T7717 |
T7718 |
compound |
DRG,neurons |
R3504 |
T7804 |
T7805 |
compound |
Akt,Akt |
R3505 |
T7805 |
T7803 |
pobj |
Akt,of |
R3506 |
T7806 |
T7805 |
punct |
/,Akt |
R3507 |
T7718 |
T7716 |
nsubj |
neurons,remain |
R3508 |
T7807 |
T7805 |
compound |
p,Akt |
R3509 |
T7808 |
T7805 |
punct |
-,Akt |
R3510 |
T7809 |
T7805 |
cc |
or,Akt |
R3511 |
T7719 |
T7718 |
prep |
from,neurons |
R3512 |
T7810 |
T7811 |
compound |
CREB,CREB |
R3513 |
T7811 |
T7805 |
conj |
CREB,Akt |
R3514 |
T7720 |
T7721 |
nmod |
TauEWS,Isl1Cre |
R3515 |
T7812 |
T7811 |
punct |
/,CREB |
R3516 |
T7813 |
T7811 |
compound |
p,CREB |
R3517 |
T7721 |
T7726 |
nmod |
Isl1Cre,embryos |
R3518 |
T7814 |
T7811 |
punct |
-,CREB |
R3519 |
T7815 |
T7802 |
prep |
in,level |
R3520 |
T7816 |
T7815 |
pobj |
DRG,in |
R3521 |
T7722 |
T7721 |
punct |
-,Isl1Cre |
R3522 |
T7817 |
T7818 |
punct |
(,6O |
R3523 |
T7818 |
T7816 |
parataxis |
6O,DRG |
R3524 |
T7723 |
T7721 |
nmod |
Pea3,Isl1Cre |
R3525 |
T7819 |
T7818 |
compound |
Figure,6O |
R3526 |
T7820 |
T7818 |
cc |
and,6O |
R3527 |
T7821 |
T7818 |
conj |
6P,6O |
R3528 |
T7724 |
T7721 |
punct |
/,Isl1Cre |
R3529 |
T7822 |
T7818 |
punct |
),6O |
R3530 |
T7823 |
T7824 |
dep |
both,shown |
R3531 |
T7725 |
T7721 |
punct |
+,Isl1Cre |
R3532 |
T7824 |
T7802 |
relcl |
shown,level |
R3533 |
T7825 |
T7823 |
prep |
of,both |
R3534 |
T7826 |
T7825 |
pobj |
which,of |
R3535 |
T7726 |
T7719 |
pobj |
embryos,from |
R3538 |
T7829 |
T7830 |
aux |
to,be |
R3539 |
T7830 |
T7824 |
xcomp |
be,shown |
R3540 |
T7831 |
T7832 |
amod |
key,regulators |
R3541 |
T7832 |
T7830 |
attr |
regulators,be |
R3542 |
T7727 |
T7721 |
punct |
/,Isl1Cre |
R3546 |
T7728 |
T7721 |
punct |
+,Isl1Cre |
R3547 |
T7836 |
T7837 |
punct |
[,29 |
R3548 |
T7837 |
T7824 |
parataxis |
29,shown |
R3549 |
T7838 |
T7837 |
punct |
],29 |
R3550 |
T7729 |
T7716 |
oprd |
post-mitotic,remain |
R3551 |
T7839 |
T7795 |
punct |
.,found |
R3552 |
T7730 |
T7716 |
cc |
but,remain |
R3553 |
T7841 |
T7842 |
advmod |
Moreover,reduced |
R3554 |
T7843 |
T7842 |
punct |
", ",reduced |
R3555 |
T7731 |
T7716 |
conj |
fail,remain |
R3556 |
T7732 |
T7733 |
aux |
to,become |
R3557 |
T7844 |
T7845 |
det |
the,level |
R3558 |
T7845 |
T7842 |
nsubjpass |
level,reduced |
R3559 |
T7733 |
T7731 |
xcomp |
become,fail |
R3560 |
T7846 |
T7845 |
prep |
of,level |
R3561 |
T7847 |
T7848 |
det |
the,Bax |
R3562 |
T7734 |
T7733 |
acomp |
sensitive,become |
R3563 |
T7848 |
T7846 |
pobj |
Bax,of |
R3564 |
T7849 |
T7848 |
amod |
proapoptotic,Bax |
R3565 |
T7850 |
T7848 |
compound |
Bcl2,Bax |
R3566 |
T7735 |
T7734 |
prep |
to,sensitive |
R3567 |
T7851 |
T7848 |
compound |
family,Bax |
R3568 |
T7852 |
T7848 |
compound |
member,Bax |
R3569 |
T7853 |
T7842 |
auxpass |
was,reduced |
R3570 |
T7736 |
T7737 |
advmod |
naturally,occurring |
R3571 |
T7854 |
T7842 |
neg |
not,reduced |
R3572 |
T7855 |
T7842 |
advmod |
significantly,reduced |
R3573 |
T7737 |
T7738 |
amod |
occurring,death |
R3574 |
T7738 |
T7735 |
pobj |
death,to |
R3575 |
T7739 |
T7738 |
compound |
cell,death |
R3576 |
T7740 |
T7731 |
punct |
", ",fail |
R3577 |
T7856 |
T7857 |
punct |
(,6O |
R3578 |
T7857 |
T7842 |
parataxis |
6O,reduced |
R3579 |
T7741 |
T7731 |
cc |
and,fail |
R3580 |
T7858 |
T7857 |
compound |
Figure,6O |
R3581 |
T7859 |
T7857 |
cc |
and,6O |
R3582 |
T7860 |
T7857 |
conj |
6P,6O |
R3583 |
T7742 |
T7731 |
conj |
survive,fail |
R3584 |
T7861 |
T7857 |
punct |
),6O |
R3585 |
T7862 |
T7842 |
punct |
.,reduced |
R3586 |
T7743 |
T7742 |
prep |
in,survive |
R3587 |
T7864 |
T7865 |
prep |
In,increased |
R3588 |
T7744 |
T7745 |
det |
the,absence |
R3589 |
T7866 |
T7864 |
pobj |
contrast,In |
R3590 |
T7867 |
T7865 |
punct |
", ",increased |
R3591 |
T7868 |
T7869 |
det |
the,level |
R3592 |
T7869 |
T7865 |
nsubjpass |
level,increased |
R3593 |
T7870 |
T7869 |
compound |
expression,level |
R3594 |
T7745 |
T7743 |
pobj |
absence,in |
R3595 |
T7871 |
T7869 |
prep |
of,level |
R3596 |
T7872 |
T7873 |
det |
the,members |
R3597 |
T7873 |
T7871 |
pobj |
members,of |
R3598 |
T7746 |
T7745 |
prep |
of,absence |
R3599 |
T7874 |
T7873 |
amod |
anti-apoptotic,members |
R3600 |
T7875 |
T7873 |
compound |
Bcl2,members |
R3601 |
T7876 |
T7873 |
compound |
family,members |
R3602 |
T7747 |
T7748 |
compound |
Trk,receptors |
R3603 |
T7877 |
T7878 |
compound |
Bcl,xl |
R3604 |
T7878 |
T7873 |
appos |
xl,members |
R3605 |
T7879 |
T7878 |
punct |
-,xl |
R3606 |
T7748 |
T7746 |
pobj |
receptors,of |
R3607 |
T7880 |
T7878 |
cc |
and,xl |
R3608 |
T7881 |
T7878 |
conj |
Bcl2,xl |
R3609 |
T7882 |
T7865 |
auxpass |
was,increased |
R3610 |
T7749 |
T7748 |
cc |
and,receptors |
R3611 |
T7883 |
T7865 |
advmod |
significantly,increased |
R3612 |
T7884 |
T7885 |
advmod |
when,compared |
R3613 |
T7885 |
T7865 |
advcl |
compared,increased |
R3614 |
T7750 |
T7751 |
amod |
neurotrophic,support |
R3615 |
T7886 |
T7885 |
prep |
to,compared |
R3616 |
T7887 |
T7888 |
amod |
wild,type |
R3617 |
T7888 |
T7890 |
compound |
type,levels |
R3618 |
T7889 |
T7888 |
punct |
-,type |
R3619 |
T7890 |
T7886 |
pobj |
levels,to |
R3620 |
T7891 |
T7892 |
punct |
(,6O |
R3621 |
T7892 |
T7890 |
parataxis |
6O,levels |
R3622 |
T7893 |
T7894 |
dep |
Bcl2,% |
R3623 |
T7894 |
T7892 |
dep |
%,6O |
R3624 |
T7895 |
T7894 |
punct |
", ",% |
R3625 |
T7896 |
T7894 |
nummod |
157,% |
R3626 |
T7897 |
T7892 |
punct |
;,6O |
R3627 |
T7898 |
T7899 |
compound |
Bcl,xl |
R3628 |
T7899 |
T7901 |
dep |
xl,% |
R3629 |
T7900 |
T7899 |
punct |
-,xl |
R3630 |
T7901 |
T7892 |
dep |
%,6O |
R3631 |
T7902 |
T7901 |
punct |
", ",% |
R3632 |
T7903 |
T7901 |
nummod |
259,% |
R3633 |
T7904 |
T7892 |
punct |
;,6O |
R3634 |
T7905 |
T7892 |
dep |
average,6O |
R3635 |
T7906 |
T7905 |
prep |
of,average |
R3636 |
T7907 |
T7908 |
nsubj |
n,experiments |
R3637 |
T7908 |
T7906 |
pcomp |
experiments,of |
R3638 |
T7909 |
T7908 |
punct |
=,experiments |
R3639 |
T7910 |
T7908 |
nummod |
3,experiments |
R3640 |
T7911 |
T7908 |
amod |
independent,experiments |
R3641 |
T7912 |
T7892 |
punct |
;,6O |
R3642 |
T7913 |
T7892 |
compound |
Figure,6O |
R3643 |
T7914 |
T7892 |
cc |
and,6O |
R3644 |
T7915 |
T7892 |
conj |
6P,6O |
R3645 |
T7916 |
T7892 |
punct |
),6O |
R3646 |
T7917 |
T7865 |
punct |
", ",increased |
R3647 |
T7918 |
T7865 |
advcl |
providing,increased |
R3648 |
T7919 |
T7920 |
det |
a,explanation |
R3649 |
T7920 |
T7918 |
dobj |
explanation,providing |
R3650 |
T7921 |
T7920 |
amod |
potential,explanation |
R3651 |
T7922 |
T7920 |
amod |
molecular,explanation |
R3652 |
T7923 |
T7920 |
prep |
for,explanation |
R3653 |
T7924 |
T7925 |
det |
the,survival |
R3654 |
T7925 |
T7923 |
pobj |
survival,for |
R3655 |
T7926 |
T7925 |
amod |
enhanced,survival |
R3656 |
T7927 |
T7925 |
amod |
neuronal,survival |
R3657 |
T7928 |
T7925 |
prep |
of,survival |
R3658 |
T7929 |
T7930 |
compound |
DRG,neurons |
R3659 |
T7930 |
T7928 |
pobj |
neurons,of |
R3660 |
T7931 |
T7930 |
prep |
of,neurons |
R3661 |
T7932 |
T7933 |
nmod |
TauEWS,Isl1Cre |
R3662 |
T7933 |
T7938 |
nmod |
Isl1Cre,embryos |
R3663 |
T7934 |
T7933 |
punct |
-,Isl1Cre |
R3664 |
T7935 |
T7933 |
nmod |
Pea3,Isl1Cre |
R3665 |
T7936 |
T7933 |
punct |
/,Isl1Cre |
R3666 |
T7937 |
T7933 |
punct |
+,Isl1Cre |
R3667 |
T7938 |
T7931 |
pobj |
embryos,of |
R3668 |
T7939 |
T7933 |
punct |
/,Isl1Cre |
R3669 |
T7940 |
T7933 |
punct |
+,Isl1Cre |
R3670 |
T7941 |
T7925 |
prep |
in,survival |
R3671 |
T7942 |
T7943 |
det |
the,absence |
R3672 |
T7943 |
T7941 |
pobj |
absence,in |
R3673 |
T7944 |
T7943 |
prep |
of,absence |
R3674 |
T7945 |
T7946 |
compound |
Trk,receptor |
R3675 |
T7946 |
T7947 |
compound |
receptor,expression |
R3676 |
T7947 |
T7944 |
pobj |
expression,of |
R3677 |
T7948 |
T7949 |
punct |
[,30 |
R3678 |
T7949 |
T7918 |
parataxis |
30,providing |
R3679 |
T7950 |
T7949 |
punct |
],30 |
R3680 |
T7951 |
T7865 |
punct |
.,increased |
R3683 |
T8705 |
T8706 |
advmod |
Only,Signaling |
R3684 |
T8706 |
T8712 |
nsubj |
Signaling,Interferes |
R3685 |
T8707 |
T8706 |
amod |
Precocious,Signaling |
R3686 |
T8708 |
T8707 |
cc |
but,Precocious |
R3687 |
T8709 |
T8708 |
neg |
Not,but |
R3688 |
T8710 |
T8707 |
conj |
Isochronic,Precocious |
R3689 |
T8711 |
T8706 |
compound |
ETS,Signaling |
R3690 |
T8713 |
T8706 |
prep |
in,Signaling |
R3691 |
T8714 |
T8715 |
compound |
DRG,Neurons |
R3692 |
T8715 |
T8713 |
pobj |
Neurons,in |
R3693 |
T8716 |
T8712 |
prep |
with,Interferes |
R3694 |
T8717 |
T8718 |
amod |
Neuronal,Fate |
R3695 |
T8718 |
T8719 |
compound |
Fate,Acquisition |
R3696 |
T8719 |
T8716 |
pobj |
Acquisition,with |
R3697 |
T8721 |
T8722 |
det |
The,differences |
R3698 |
T8722 |
T8724 |
nsubj |
differences,prompted |
R3699 |
T8723 |
T8722 |
amod |
observed,differences |
R3700 |
T8725 |
T8722 |
prep |
in,differences |
R3701 |
T8726 |
T8727 |
amod |
neuronal,survival |
R3702 |
T8727 |
T8725 |
pobj |
survival,in |
R3703 |
T8728 |
T8727 |
cc |
and,survival |
R3704 |
T8729 |
T8730 |
compound |
neurite,outgrowth |
R3705 |
T8730 |
T8727 |
conj |
outgrowth,survival |
R3706 |
T8731 |
T8722 |
prep |
between,differences |
R3707 |
T8732 |
T8733 |
amod |
precocious,expression |
R3708 |
T8733 |
T8731 |
pobj |
expression,between |
R3709 |
T8734 |
T8732 |
cc |
and,precocious |
R3710 |
T8735 |
T8732 |
conj |
isochronic,precocious |
R3711 |
T8736 |
T8733 |
prep |
of,expression |
R3712 |
T8737 |
T8738 |
compound |
EWS,Pea3 |
R3713 |
T8738 |
T8736 |
pobj |
Pea3,of |
R3714 |
T8739 |
T8738 |
punct |
-,Pea3 |
R3715 |
T8740 |
T8724 |
dobj |
us,prompted |
R3716 |
T8741 |
T8742 |
aux |
to,perform |
R3717 |
T8742 |
T8724 |
xcomp |
perform,prompted |
R3718 |
T8743 |
T8744 |
det |
a,analysis |
R3719 |
T8744 |
T8742 |
dobj |
analysis,perform |
R3720 |
T8745 |
T8744 |
amod |
direct,analysis |
R3721 |
T8746 |
T8744 |
amod |
comparative,analysis |
R3722 |
T8747 |
T8744 |
prep |
of,analysis |
R3723 |
T8748 |
T8749 |
compound |
gene,expression |
R3724 |
T8749 |
T8747 |
pobj |
expression,of |
R3725 |
T8750 |
T8749 |
prep |
between,expression |
R3726 |
T8751 |
T8750 |
pobj |
mice,between |
R3727 |
T8752 |
T8751 |
prep |
with,mice |
R3728 |
T8753 |
T8754 |
amod |
precocious,expression |
R3729 |
T8754 |
T8752 |
pobj |
expression,with |
R3730 |
T8755 |
T8756 |
compound |
EWS,Pea3 |
R3731 |
T8756 |
T8754 |
compound |
Pea3,expression |
R3732 |
T8757 |
T8756 |
punct |
-,Pea3 |
R3733 |
T8758 |
T8751 |
cc |
and,mice |
R3734 |
T8759 |
T8751 |
conj |
mice,mice |
R3735 |
T8760 |
T8761 |
prep |
in,initiated |
R3736 |
T8761 |
T8759 |
relcl |
initiated,mice |
R3737 |
T8762 |
T8760 |
pobj |
which,in |
R3738 |
T8763 |
T8764 |
det |
the,expression |
R3739 |
T8764 |
T8761 |
nsubjpass |
expression,initiated |
R3740 |
T8765 |
T8764 |
prep |
of,expression |
R3741 |
T8766 |
T8767 |
compound |
EWS,Pea3 |
R3742 |
T8767 |
T8765 |
pobj |
Pea3,of |
R3743 |
T8768 |
T8767 |
punct |
-,Pea3 |
R3744 |
T8769 |
T8761 |
auxpass |
is,initiated |
R3745 |
T8770 |
T8761 |
prep |
in,initiated |
R3746 |
T8771 |
T8772 |
nmod |
DRG,neurons |
R3747 |
T8772 |
T8770 |
pobj |
neurons,in |
R3748 |
T8773 |
T8772 |
amod |
sensory,neurons |
R3749 |
T8774 |
T8761 |
prep |
from,initiated |
R3750 |
T8775 |
T8776 |
det |
the,time |
R3751 |
T8776 |
T8774 |
pobj |
time,from |
R3752 |
T8777 |
T8776 |
prep |
of,time |
R3753 |
T8778 |
T8779 |
amod |
normal,onset |
R3754 |
T8779 |
T8777 |
pobj |
onset,of |
R3755 |
T8780 |
T8779 |
prep |
of,onset |
R3756 |
T8781 |
T8782 |
compound |
Er81,expression |
R3757 |
T8782 |
T8780 |
pobj |
expression,of |
R3758 |
T8783 |
T8724 |
punct |
.,prompted |
R3759 |
T8785 |
T8786 |
advmod |
Moreover,performed |
R3760 |
T8787 |
T8786 |
punct |
", ",performed |
R3761 |
T8788 |
T8789 |
aux |
to,rule |
R3762 |
T8789 |
T8786 |
advcl |
rule,performed |
R3763 |
T8790 |
T8789 |
prt |
out,rule |
R3764 |
T8791 |
T8792 |
det |
the,possibility |
R3765 |
T8792 |
T8789 |
dobj |
possibility,rule |
R3766 |
T8793 |
T8794 |
mark |
that,be |
R3767 |
T8794 |
T8792 |
acl |
be,possibility |
R3768 |
T8795 |
T8796 |
det |
a,effect |
R3769 |
T8796 |
T8794 |
nsubj |
effect,be |
R3770 |
T8797 |
T8796 |
amod |
differential,effect |
R3771 |
T8798 |
T8794 |
aux |
may,be |
R3772 |
T8799 |
T8794 |
prep |
due,be |
R3773 |
T8800 |
T8799 |
pcomp |
to,due |
R3774 |
T8801 |
T8802 |
det |
the,strategies |
R3775 |
T8802 |
T8799 |
pobj |
strategies,due |
R3776 |
T8803 |
T8802 |
amod |
different,strategies |
R3777 |
T8804 |
T8802 |
amod |
genetic,strategies |
R3778 |
T8805 |
T8806 |
prep |
by,achieved |
R3779 |
T8806 |
T8802 |
relcl |
achieved,strategies |
R3780 |
T8807 |
T8805 |
pobj |
which,by |
R3781 |
T8808 |
T8806 |
nsubjpass |
expression,achieved |
R3782 |
T8809 |
T8808 |
prep |
of,expression |
R3783 |
T8810 |
T8811 |
compound |
EWS,Pea3 |
R3784 |
T8811 |
T8809 |
pobj |
Pea3,of |
R3785 |
T8812 |
T8811 |
punct |
-,Pea3 |
R3786 |
T8813 |
T8808 |
prep |
in,expression |
R3787 |
T8814 |
T8815 |
amod |
proprioceptive,afferents |
R3788 |
T8815 |
T8813 |
pobj |
afferents,in |
R3789 |
T8816 |
T8806 |
auxpass |
is,achieved |
R3790 |
T8817 |
T8786 |
punct |
", ",performed |
R3791 |
T8818 |
T8786 |
nsubj |
we,performed |
R3792 |
T8819 |
T8820 |
det |
this,analysis |
R3793 |
T8820 |
T8786 |
dobj |
analysis,performed |
R3794 |
T8821 |
T8822 |
preconj |
both,in |
R3795 |
T8822 |
T8786 |
prep |
in,performed |
R3796 |
T8823 |
T8824 |
nmod |
Er81EWS,Pea3 |
R3797 |
T8824 |
T8826 |
nmod |
Pea3,embryos |
R3798 |
T8825 |
T8824 |
punct |
-,Pea3 |
R3799 |
T8826 |
T8822 |
pobj |
embryos,in |
R3800 |
T8827 |
T8824 |
punct |
/,Pea3 |
R3801 |
T8828 |
T8824 |
punct |
−,Pea3 |
R3802 |
T8829 |
T8824 |
cc |
and,Pea3 |
R3803 |
T8830 |
T8831 |
nmod |
TauEWS,PVCre |
R3804 |
T8831 |
T8824 |
conj |
PVCre,Pea3 |
R3805 |
T8832 |
T8831 |
punct |
-,PVCre |
R3806 |
T8833 |
T8831 |
nmod |
Pea3,PVCre |
R3807 |
T8834 |
T8831 |
punct |
/,PVCre |
R3808 |
T8835 |
T8831 |
punct |
+,PVCre |
R3809 |
T8836 |
T8831 |
punct |
/,PVCre |
R3810 |
T8837 |
T8831 |
punct |
+,PVCre |
R3811 |
T8838 |
T8786 |
punct |
.,performed |
R3812 |
T8840 |
T8841 |
nsubj |
We,analyzed |
R3813 |
T8842 |
T8841 |
advmod |
first,analyzed |
R3814 |
T8843 |
T8841 |
dobj |
expression,analyzed |
R3815 |
T8844 |
T8843 |
prep |
of,expression |
R3816 |
T8845 |
T8844 |
pobj |
TrkC,of |
R3817 |
T8846 |
T8845 |
punct |
", ",TrkC |
R3818 |
T8847 |
T8848 |
det |
a,gene |
R3819 |
T8848 |
T8845 |
appos |
gene,TrkC |
R3820 |
T8849 |
T8848 |
acl |
downregulated,gene |
R3821 |
T8850 |
T8849 |
prep |
in,downregulated |
R3822 |
T8851 |
T8852 |
compound |
DRG,neurons |
R3823 |
T8852 |
T8850 |
pobj |
neurons,in |
R3824 |
T8853 |
T8852 |
prep |
of,neurons |
R3825 |
T8854 |
T8855 |
nmod |
TauEWS,Isl1Cre |
R3826 |
T8855 |
T8860 |
nmod |
Isl1Cre,embryos |
R3827 |
T8856 |
T8855 |
punct |
-,Isl1Cre |
R3828 |
T8857 |
T8855 |
nmod |
Pea3,Isl1Cre |
R3829 |
T8858 |
T8855 |
punct |
/,Isl1Cre |
R3830 |
T8859 |
T8855 |
punct |
+,Isl1Cre |
R3831 |
T8860 |
T8853 |
pobj |
embryos,of |
R3832 |
T8861 |
T8855 |
punct |
/,Isl1Cre |
R3833 |
T8862 |
T8855 |
punct |
+,Isl1Cre |
R3834 |
T8863 |
T8864 |
punct |
(,7A |
R3835 |
T8864 |
T8841 |
parataxis |
7A,analyzed |
R3836 |
T8865 |
T8864 |
compound |
Figure,7A |
R3837 |
T8866 |
T8864 |
cc |
and,7A |
R3838 |
T8867 |
T8864 |
conj |
7B,7A |
R3839 |
T8868 |
T8864 |
punct |
),7A |
R3840 |
T8869 |
T8841 |
punct |
.,analyzed |
R3841 |
T8871 |
T8872 |
det |
The,level |
R3842 |
T8872 |
T8873 |
nsubj |
level,was |
R3843 |
T8874 |
T8872 |
prep |
of,level |
R3844 |
T8875 |
T8874 |
pobj |
expression,of |
R3845 |
T8876 |
T8875 |
prep |
of,expression |
R3846 |
T8877 |
T8876 |
pobj |
TrkC,of |
R3847 |
T8878 |
T8873 |
acomp |
indistinguishable,was |
R3848 |
T8879 |
T8878 |
prep |
from,indistinguishable |
R3849 |
T8880 |
T8881 |
amod |
wild,type |
R3850 |
T8881 |
T8879 |
pobj |
type,from |
R3851 |
T8882 |
T8881 |
punct |
-,type |
R3852 |
T8883 |
T8873 |
prep |
in,was |
R3853 |
T8884 |
T8885 |
compound |
DRG,neurons |
R3854 |
T8885 |
T8883 |
pobj |
neurons,in |
R3855 |
T8886 |
T8885 |
prep |
of,neurons |
R3856 |
T8887 |
T8888 |
nmod |
Er81EWS,Pea3 |
R3857 |
T8888 |
T8890 |
nmod |
Pea3,embryos |
R3858 |
T8889 |
T8888 |
punct |
-,Pea3 |
R3859 |
T8890 |
T8886 |
pobj |
embryos,of |
R3860 |
T8891 |
T8888 |
punct |
/,Pea3 |
R3861 |
T8892 |
T8888 |
punct |
−,Pea3 |
R3862 |
T8893 |
T8888 |
cc |
and,Pea3 |
R3863 |
T8894 |
T8895 |
nmod |
TauEWS,PVCre |
R3864 |
T8895 |
T8888 |
conj |
PVCre,Pea3 |
R3865 |
T8896 |
T8895 |
punct |
-,PVCre |
R3866 |
T8897 |
T8895 |
nmod |
Pea3,PVCre |
R3867 |
T8898 |
T8895 |
punct |
/,PVCre |
R3868 |
T8899 |
T8895 |
punct |
+,PVCre |
R3869 |
T8900 |
T8895 |
punct |
/,PVCre |
R3870 |
T8901 |
T8895 |
punct |
+,PVCre |
R3871 |
T8902 |
T8903 |
punct |
(,7A |
R3872 |
T8903 |
T8873 |
parataxis |
7A,was |
R3873 |
T8904 |
T8903 |
compound |
Figure,7A |
R3874 |
T8905 |
T8903 |
punct |
", ",7A |
R3875 |
T8906 |
T8903 |
conj |
7C,7A |
R3876 |
T8907 |
T8906 |
punct |
", ",7C |
R3877 |
T8908 |
T8906 |
cc |
and,7C |
R3878 |
T8909 |
T8906 |
conj |
7D,7C |
R3879 |
T8910 |
T8903 |
punct |
),7A |
R3880 |
T8911 |
T8873 |
punct |
.,was |
R3881 |
T8913 |
T8914 |
advmod |
Moreover,expressed |
R3882 |
T8915 |
T8914 |
punct |
", ",expressed |
R3883 |
T8916 |
T8914 |
nsubjpass |
PV,expressed |
R3884 |
T8917 |
T8914 |
auxpass |
was,expressed |
R3885 |
T8918 |
T8914 |
neg |
not,expressed |
R3886 |
T8919 |
T8914 |
prep |
in,expressed |
R3887 |
T8920 |
T8921 |
compound |
DRG,neurons |
R3888 |
T8921 |
T8919 |
pobj |
neurons,in |
R3889 |
T8922 |
T8921 |
prep |
of,neurons |
R3890 |
T8923 |
T8924 |
nmod |
TauEWS,Isl1Cre |
R3891 |
T8924 |
T8929 |
nmod |
Isl1Cre,embryos |
R3892 |
T8925 |
T8924 |
punct |
-,Isl1Cre |
R3893 |
T8926 |
T8924 |
nmod |
Pea3,Isl1Cre |
R3894 |
T8927 |
T8924 |
punct |
/,Isl1Cre |
R3895 |
T8928 |
T8924 |
punct |
+,Isl1Cre |
R3896 |
T8929 |
T8922 |
pobj |
embryos,of |
R3897 |
T8930 |
T8924 |
punct |
/,Isl1Cre |
R3898 |
T8931 |
T8924 |
punct |
+,Isl1Cre |
R3899 |
T8932 |
T8933 |
punct |
(,S5 |
R3900 |
T8933 |
T8914 |
parataxis |
S5,expressed |
R3901 |
T8934 |
T8933 |
compound |
Figure,S5 |
R3902 |
T8935 |
T8933 |
punct |
),S5 |
R3903 |
T8936 |
T8914 |
cc |
but,expressed |
R3904 |
T8937 |
T8938 |
auxpass |
was,expressed |
R3905 |
T8938 |
T8914 |
conj |
expressed,expressed |
R3906 |
T8939 |
T8938 |
agent |
by,expressed |
R3907 |
T8940 |
T8941 |
amod |
proprioceptive,afferents |
R3908 |
T8941 |
T8939 |
pobj |
afferents,by |
R3909 |
T8942 |
T8938 |
prep |
in,expressed |
R3910 |
T8943 |
T8944 |
preconj |
both,type |
R3911 |
T8944 |
T8947 |
nmod |
type,embryos |
R3912 |
T8945 |
T8944 |
amod |
wild,type |
R3913 |
T8946 |
T8944 |
punct |
-,type |
R3914 |
T8947 |
T8942 |
pobj |
embryos,in |
R3915 |
T8948 |
T8944 |
cc |
and,type |
R3916 |
T8949 |
T8950 |
compound |
Er81EWS,Pea3 |
R3917 |
T8950 |
T8944 |
conj |
Pea3,type |
R3918 |
T8951 |
T8950 |
punct |
-,Pea3 |
R3919 |
T8952 |
T8950 |
punct |
/,Pea3 |
R3920 |
T8953 |
T8950 |
punct |
−,Pea3 |
R3921 |
T8954 |
T8955 |
punct |
(,see |
R3922 |
T8955 |
T8938 |
parataxis |
see,expressed |
R3923 |
T8956 |
T8957 |
nmod |
Figures,S5 |
R3924 |
T8957 |
T8955 |
dobj |
S5,see |
R3925 |
T8958 |
T8957 |
nummod |
1,S5 |
R3926 |
T8959 |
T8957 |
cc |
and,S5 |
R3927 |
T8960 |
T8955 |
punct |
),see |
R3928 |
T8961 |
T8962 |
punct |
[,14 |
R3929 |
T8962 |
T8938 |
parataxis |
14,expressed |
R3930 |
T8963 |
T8962 |
punct |
],14 |
R3931 |
T8964 |
T8914 |
punct |
.,expressed |
R3932 |
T8966 |
T8967 |
nsubj |
We,found |
R3933 |
T8968 |
T8967 |
advmod |
also,found |
R3934 |
T8969 |
T8970 |
amod |
several,genes |
R3935 |
T8970 |
T8967 |
dobj |
genes,found |
R3936 |
T8971 |
T8972 |
dep |
that,upregulated |
R3937 |
T8972 |
T8970 |
relcl |
upregulated,genes |
R3938 |
T8973 |
T8972 |
auxpass |
were,upregulated |
R3939 |
T8974 |
T8972 |
advmod |
ectopically,upregulated |
R3940 |
T8975 |
T8972 |
prep |
in,upregulated |
R3941 |
T8976 |
T8977 |
compound |
DRG,neurons |
R3942 |
T8977 |
T8975 |
pobj |
neurons,in |
R3943 |
T8978 |
T8977 |
prep |
of,neurons |
R3944 |
T8979 |
T8980 |
nmod |
TauEWS,Isl1Cre |
R3945 |
T8980 |
T8985 |
nmod |
Isl1Cre,embryos |
R3946 |
T8981 |
T8980 |
punct |
-,Isl1Cre |
R3947 |
T8982 |
T8980 |
nmod |
Pea3,Isl1Cre |
R3948 |
T8983 |
T8980 |
punct |
/,Isl1Cre |
R3949 |
T8984 |
T8980 |
punct |
+,Isl1Cre |
R3950 |
T8985 |
T8978 |
pobj |
embryos,of |
R3951 |
T8986 |
T8980 |
punct |
/,Isl1Cre |
R3952 |
T8987 |
T8980 |
punct |
+,Isl1Cre |
R3953 |
T8988 |
T8989 |
punct |
(,Figure |
R3954 |
T8989 |
T8972 |
parataxis |
Figure,upregulated |
R3955 |
T8990 |
T8989 |
nummod |
7,Figure |
R3956 |
T8991 |
T8989 |
punct |
),Figure |
R3957 |
T8992 |
T8967 |
punct |
.,found |
R3958 |
T8994 |
T8995 |
nsubjpass |
Calretinin,induced |
R3959 |
T8996 |
T8994 |
cc |
and,Calretinin |
R3960 |
T8997 |
T8994 |
conj |
Calbindin,Calretinin |
R3961 |
T8998 |
T8994 |
punct |
", ",Calretinin |
R3962 |
T8999 |
T9000 |
nummod |
two,proteins |
R3963 |
T9000 |
T8994 |
appos |
proteins,Calretinin |
R3964 |
T9001 |
T9000 |
amod |
different,proteins |
R3965 |
T9002 |
T9003 |
npadvmod |
calcium,binding |
R3966 |
T9003 |
T9000 |
amod |
binding,proteins |
R3967 |
T9004 |
T9003 |
punct |
-,binding |
R3968 |
T9005 |
T9000 |
acl |
expressed,proteins |
R3969 |
T9006 |
T9005 |
agent |
by,expressed |
R3970 |
T9007 |
T9006 |
pobj |
subpopulations,by |
R3971 |
T9008 |
T9007 |
prep |
of,subpopulations |
R3972 |
T9009 |
T9010 |
compound |
DRG,neurons |
R3973 |
T9010 |
T9008 |
pobj |
neurons,of |
R3974 |
T9011 |
T9007 |
prep |
in,subpopulations |
R3975 |
T9012 |
T9013 |
amod |
wild,type |
R3976 |
T9013 |
T9015 |
nmod |
type,embryos |
R3977 |
T9014 |
T9013 |
punct |
-,type |
R3978 |
T9015 |
T9011 |
pobj |
embryos,in |
R3979 |
T9016 |
T9013 |
punct |
", ",type |
R3980 |
T9017 |
T9018 |
compound |
Er81EWS,Pea3 |
R3981 |
T9018 |
T9013 |
conj |
Pea3,type |
R3982 |
T9019 |
T9018 |
punct |
-,Pea3 |
R3983 |
T9020 |
T9018 |
punct |
/,Pea3 |
R3984 |
T9021 |
T9018 |
punct |
−,Pea3 |
R3985 |
T9022 |
T9018 |
punct |
", ",Pea3 |
R3986 |
T9023 |
T9018 |
cc |
and,Pea3 |
R3987 |
T9024 |
T9025 |
nmod |
TauEWS,PVCre |
R3988 |
T9025 |
T9018 |
conj |
PVCre,Pea3 |
R3989 |
T9026 |
T9025 |
punct |
-,PVCre |
R3990 |
T9027 |
T9025 |
nmod |
Pea3,PVCre |
R3991 |
T9028 |
T9025 |
punct |
/,PVCre |
R3992 |
T9029 |
T9025 |
punct |
+,PVCre |
R3993 |
T9030 |
T9025 |
punct |
/,PVCre |
R3994 |
T9031 |
T9025 |
punct |
+,PVCre |
R3995 |
T9032 |
T9033 |
punct |
(,7E |
R3996 |
T9033 |
T8994 |
parataxis |
7E,Calretinin |
R3997 |
T9034 |
T9033 |
compound |
Figure,7E |
R3998 |
T9035 |
T9033 |
punct |
", ",7E |
R3999 |
T9036 |
T9033 |
conj |
7G,7E |
R4000 |
T9037 |
T9036 |
punct |
", ",7G |
R4001 |
T9038 |
T9036 |
cc |
and,7G |
R4002 |
T9039 |
T9036 |
conj |
7H,7G |
R4003 |
T9040 |
T9033 |
punct |
;,7E |
R4004 |
T9041 |
T9042 |
nsubj |
data,shown |
R4005 |
T9042 |
T9033 |
ccomp |
shown,7E |
R4006 |
T9043 |
T9042 |
neg |
not,shown |
R4007 |
T9044 |
T9033 |
punct |
),7E |
R4008 |
T9045 |
T9046 |
punct |
[,32 |
R4009 |
T9046 |
T8994 |
parataxis |
32,Calretinin |
R4010 |
T9047 |
T9046 |
nummod |
31,32 |
R4011 |
T9048 |
T9046 |
punct |
",",32 |
R4012 |
T9049 |
T9046 |
punct |
],32 |
R4013 |
T9050 |
T8995 |
punct |
", ",induced |
R4014 |
T9051 |
T8995 |
auxpass |
were,induced |
R4015 |
T9052 |
T8995 |
prep |
in,induced |
R4016 |
T9053 |
T9054 |
amod |
more,95 |
R4017 |
T9054 |
T9056 |
nummod |
95,% |
R4018 |
T9055 |
T9054 |
quantmod |
than,95 |
R4019 |
T9056 |
T9052 |
pobj |
%,in |
R4020 |
T9057 |
T9056 |
prep |
of,% |
R4021 |
T9058 |
T9059 |
det |
all,neurons |
R4022 |
T9059 |
T9057 |
pobj |
neurons,of |
R4023 |
T9060 |
T9059 |
compound |
DRG,neurons |
R4024 |
T9061 |
T9059 |
prep |
of,neurons |
R4025 |
T9062 |
T9063 |
nmod |
TauEWS,Isl1Cre |
R4026 |
T9063 |
T9068 |
nmod |
Isl1Cre,embryos |
R4027 |
T9064 |
T9063 |
punct |
-,Isl1Cre |
R4028 |
T9065 |
T9063 |
nmod |
Pea3,Isl1Cre |
R4029 |
T9066 |
T9063 |
punct |
/,Isl1Cre |
R4030 |
T9067 |
T9063 |
punct |
+,Isl1Cre |
R4031 |
T9068 |
T9061 |
pobj |
embryos,of |
R4032 |
T9069 |
T9063 |
punct |
/,Isl1Cre |
R4033 |
T9070 |
T9063 |
punct |
+,Isl1Cre |
R4034 |
T9071 |
T9072 |
punct |
(,7F |
R4035 |
T9072 |
T8995 |
parataxis |
7F,induced |
R4036 |
T9073 |
T9072 |
compound |
Figures,7F |
R4037 |
T9074 |
T9072 |
cc |
and,7F |
R4038 |
T9075 |
T9072 |
conj |
S5,7F |
R4039 |
T9076 |
T9072 |
punct |
),7F |
R4040 |
T9077 |
T8995 |
punct |
.,induced |
R4041 |
T9079 |
T9080 |
det |
These,findings |
R4042 |
T9080 |
T9081 |
nsubj |
findings,suggest |
R4043 |
T9082 |
T9083 |
mark |
that,fail |
R4044 |
T9083 |
T9081 |
ccomp |
fail,suggest |
R4045 |
T9084 |
T9085 |
compound |
DRG,neurons |
R4046 |
T9085 |
T9083 |
nsubj |
neurons,fail |
R4047 |
T9086 |
T9085 |
prep |
in,neurons |
R4048 |
T9087 |
T9088 |
nmod |
TauEWS,Isl1Cre |
R4049 |
T9088 |
T9093 |
nmod |
Isl1Cre,embryos |
R4050 |
T9089 |
T9088 |
punct |
-,Isl1Cre |
R4051 |
T9090 |
T9088 |
nmod |
Pea3,Isl1Cre |
R4052 |
T9091 |
T9088 |
punct |
/,Isl1Cre |
R4053 |
T9092 |
T9088 |
punct |
+,Isl1Cre |
R4054 |
T9093 |
T9086 |
pobj |
embryos,in |
R4055 |
T9094 |
T9088 |
punct |
/,Isl1Cre |
R4056 |
T9095 |
T9088 |
punct |
+,Isl1Cre |
R4057 |
T9096 |
T9097 |
aux |
to,differentiate |
R4058 |
T9097 |
T9083 |
xcomp |
differentiate,fail |
R4059 |
T9098 |
T9097 |
prep |
to,differentiate |
R4060 |
T9099 |
T9100 |
det |
a,fate |
R4061 |
T9100 |
T9098 |
pobj |
fate,to |
R4062 |
T9101 |
T9100 |
amod |
normal,fate |
R4063 |
T9102 |
T9083 |
cc |
and,fail |
R4064 |
T9103 |
T9104 |
advmod |
instead,acquire |
R4065 |
T9104 |
T9083 |
conj |
acquire,fail |
R4066 |
T9105 |
T9106 |
det |
an,identity |
R4067 |
T9106 |
T9104 |
dobj |
identity,acquire |
R4068 |
T9107 |
T9106 |
amod |
aberrant,identity |
R4069 |
T9108 |
T9106 |
amod |
distinct,identity |
R4070 |
T9109 |
T9108 |
prep |
from,distinct |
R4071 |
T9110 |
T9111 |
det |
any,subpopulation |
R4072 |
T9111 |
T9109 |
pobj |
subpopulation,from |
R4073 |
T9112 |
T9111 |
prep |
of,subpopulation |
R4074 |
T9113 |
T9114 |
amod |
wild,type |
R4075 |
T9114 |
T9116 |
compound |
type,neurons |
R4076 |
T9115 |
T9114 |
punct |
-,type |
R4077 |
T9116 |
T9112 |
pobj |
neurons,of |
R4078 |
T9117 |
T9116 |
compound |
DRG,neurons |
R4079 |
T9118 |
T9081 |
punct |
.,suggest |
R4080 |
T9120 |
T9121 |
advmod |
Finally,activated |
R4081 |
T9122 |
T9121 |
punct |
", ",activated |
R4082 |
T9123 |
T9124 |
aux |
to,assess |
R4083 |
T9124 |
T9121 |
advcl |
assess,activated |
R4084 |
T9125 |
T9126 |
mark |
whether,acts |
R4085 |
T9126 |
T9124 |
dobj |
acts,assess |
R4086 |
T9127 |
T9128 |
compound |
EWS,Pea3 |
R4087 |
T9128 |
T9126 |
nsubj |
Pea3,acts |
R4088 |
T9129 |
T9128 |
punct |
-,Pea3 |
R4089 |
T9130 |
T9128 |
acl |
expressed,Pea3 |
R4090 |
T9131 |
T9130 |
advmod |
precociously,expressed |
R4091 |
T9132 |
T9133 |
advmod |
exclusively,autonomously |
R4092 |
T9133 |
T9126 |
advmod |
autonomously,acts |
R4093 |
T9134 |
T9133 |
npadvmod |
cell,autonomously |
R4094 |
T9135 |
T9133 |
punct |
-,autonomously |
R4095 |
T9136 |
T9126 |
cc |
or,acts |
R4096 |
T9137 |
T9138 |
mark |
whether,influence |
R4097 |
T9138 |
T9126 |
conj |
influence,acts |
R4098 |
T9139 |
T9138 |
nsubj |
it,influence |
R4099 |
T9140 |
T9138 |
aux |
may,influence |
R4100 |
T9141 |
T9138 |
advmod |
also,influence |
R4101 |
T9142 |
T9143 |
amod |
neighboring,neurons |
R4102 |
T9143 |
T9138 |
dobj |
neurons,influence |
R4103 |
T9144 |
T9143 |
compound |
DRG,neurons |
R4104 |
T9145 |
T9121 |
punct |
", ",activated |
R4105 |
T9146 |
T9121 |
nsubj |
we,activated |
R4106 |
T9147 |
T9121 |
dobj |
expression,activated |
R4107 |
T9148 |
T9147 |
prep |
of,expression |
R4108 |
T9149 |
T9150 |
compound |
EWS,Pea3 |
R4109 |
T9150 |
T9148 |
pobj |
Pea3,of |
R4110 |
T9151 |
T9150 |
punct |
-,Pea3 |
R4111 |
T9152 |
T9121 |
advcl |
using,activated |
R4112 |
T9153 |
T9154 |
compound |
Hb9Cre,mice |
R4113 |
T9154 |
T9152 |
dobj |
mice,using |
R4114 |
T9155 |
T9156 |
punct |
[,33 |
R4115 |
T9156 |
T9121 |
parataxis |
33,activated |
R4116 |
T9157 |
T9156 |
punct |
],33 |
R4117 |
T9158 |
T9121 |
punct |
.,activated |
R4118 |
T9160 |
T9161 |
prep |
Due,undergo |
R4119 |
T9162 |
T9160 |
pcomp |
to,Due |
R4120 |
T9163 |
T9164 |
det |
a,expression |
R4121 |
T9164 |
T9160 |
pobj |
expression,Due |
R4122 |
T9165 |
T9164 |
amod |
transient,expression |
R4123 |
T9166 |
T9165 |
cc |
and,transient |
R4124 |
T9167 |
T9168 |
advmod |
rostro,caudally |
R4125 |
T9168 |
T9170 |
advmod |
caudally,graded |
R4126 |
T9169 |
T9168 |
punct |
-,caudally |
R4127 |
T9170 |
T9165 |
conj |
graded,transient |
R4128 |
T9171 |
T9164 |
prep |
of,expression |
R4129 |
T9172 |
T9171 |
pobj |
Hb9,of |
R4130 |
T9173 |
T9164 |
prep |
at,expression |
R4131 |
T9174 |
T9175 |
amod |
neural,plate |
R4132 |
T9175 |
T9176 |
compound |
plate,stages |
R4133 |
T9176 |
T9173 |
pobj |
stages,at |
R4134 |
T9177 |
T9161 |
punct |
", ",undergo |
R4135 |
T9178 |
T9179 |
advmod |
very,few |
R4136 |
T9179 |
T9180 |
amod |
few,neurons |
R4137 |
T9180 |
T9161 |
nsubj |
neurons,undergo |
R4138 |
T9181 |
T9180 |
compound |
DRG,neurons |
R4139 |
T9182 |
T9180 |
prep |
at,neurons |
R4140 |
T9183 |
T9184 |
amod |
brachial,levels |
R4141 |
T9184 |
T9182 |
pobj |
levels,at |
R4142 |
T9185 |
T9180 |
cc |
and,neurons |
R4143 |
T9186 |
T9187 |
advmod |
increasingly,more |
R4144 |
T9187 |
T9188 |
amod |
more,neurons |
R4145 |
T9188 |
T9180 |
conj |
neurons,neurons |
R4146 |
T9189 |
T9188 |
acl |
progressing,neurons |
R4147 |
T9190 |
T9189 |
advmod |
caudally,progressing |
R4148 |
T9191 |
T9161 |
dobj |
recombination,undergo |
R4149 |
T9192 |
T9161 |
prep |
in,undergo |
R4150 |
T9193 |
T9194 |
nmod |
TauEWS,Hb9Cre |
R4151 |
T9194 |
T9199 |
nmod |
Hb9Cre,embryos |
R4152 |
T9195 |
T9194 |
punct |
-,Hb9Cre |
R4153 |
T9196 |
T9194 |
nmod |
Pea3,Hb9Cre |
R4154 |
T9197 |
T9194 |
punct |
/,Hb9Cre |
R4155 |
T9198 |
T9194 |
punct |
+,Hb9Cre |
R4156 |
T9199 |
T9192 |
pobj |
embryos,in |
R4157 |
T9200 |
T9194 |
punct |
/,Hb9Cre |
R4158 |
T9201 |
T9194 |
punct |
+,Hb9Cre |
R4159 |
T9202 |
T9194 |
cc |
and,Hb9Cre |
R4160 |
T9203 |
T9204 |
nmod |
TaumGFP,Hb9Cre |
R4161 |
T9204 |
T9194 |
conj |
Hb9Cre,Hb9Cre |
R4162 |
T9205 |
T9204 |
punct |
/,Hb9Cre |
R4163 |
T9206 |
T9204 |
punct |
+,Hb9Cre |
R4164 |
T9207 |
T9204 |
punct |
/,Hb9Cre |
R4165 |
T9208 |
T9204 |
punct |
+,Hb9Cre |
R4166 |
T9209 |
T9210 |
punct |
(,Figure |
R4167 |
T9210 |
T9161 |
parataxis |
Figure,undergo |
R4168 |
T9211 |
T9210 |
nummod |
8,Figure |
R4169 |
T9212 |
T9210 |
punct |
),Figure |
R4170 |
T9213 |
T9161 |
punct |
.,undergo |
R4171 |
T9215 |
T9216 |
advmod |
Nevertheless,restricted |
R4172 |
T9217 |
T9216 |
punct |
", ",restricted |
R4173 |
T9218 |
T9216 |
nsubjpass |
downregulation,restricted |
R4174 |
T9219 |
T9218 |
prep |
of,downregulation |
R4175 |
T9220 |
T9221 |
compound |
Trk,expression |
R4176 |
T9221 |
T9219 |
pobj |
expression,of |
R4177 |
T9222 |
T9221 |
compound |
receptor,expression |
R4178 |
T9223 |
T9221 |
cc |
or,expression |
R4179 |
T9224 |
T9221 |
conj |
upregulation,expression |
R4180 |
T9225 |
T9221 |
prep |
of,expression |
R4181 |
T9226 |
T9225 |
pobj |
Calretinin,of |
R4182 |
T9227 |
T9216 |
auxpass |
is,restricted |
R4183 |
T9228 |
T9216 |
advmod |
exclusively,restricted |
R4184 |
T9229 |
T9216 |
prep |
to,restricted |
R4185 |
T9230 |
T9229 |
pobj |
neurons,to |
R4186 |
T9231 |
T9232 |
dep |
that,undergone |
R4187 |
T9232 |
T9230 |
relcl |
undergone,neurons |
R4188 |
T9233 |
T9232 |
aux |
have,undergone |
R4189 |
T9234 |
T9232 |
dobj |
recombination,undergone |
R4190 |
T9235 |
T9232 |
cc |
and,undergone |
R4191 |
T9236 |
T9237 |
aux |
can,observed |
R4192 |
T9237 |
T9232 |
conj |
observed,undergone |
R4193 |
T9238 |
T9237 |
neg |
not,observed |
R4194 |
T9239 |
T9237 |
auxpass |
be,observed |
R4195 |
T9240 |
T9237 |
prep |
in,observed |
R4196 |
T9241 |
T9242 |
nmod |
TaumGFP,Hb9Cre |
R4197 |
T9242 |
T9245 |
nmod |
Hb9Cre,embryos |
R4198 |
T9243 |
T9242 |
punct |
/,Hb9Cre |
R4199 |
T9244 |
T9242 |
punct |
+,Hb9Cre |
R4200 |
T9245 |
T9240 |
pobj |
embryos,in |
R4201 |
T9246 |
T9242 |
punct |
/,Hb9Cre |
R4202 |
T9247 |
T9242 |
punct |
+,Hb9Cre |
R4203 |
T9248 |
T9249 |
punct |
(,Figure |
R4204 |
T9249 |
T9237 |
parataxis |
Figure,observed |
R4205 |
T9250 |
T9249 |
nummod |
8,Figure |
R4206 |
T9251 |
T9249 |
punct |
),Figure |
R4207 |
T9252 |
T9216 |
punct |
.,restricted |
R4208 |
T9254 |
T9255 |
advmod |
Together,demonstrate |
R4209 |
T9256 |
T9255 |
punct |
", ",demonstrate |
R4210 |
T9257 |
T9258 |
det |
these,results |
R4211 |
T9258 |
T9255 |
nsubj |
results,demonstrate |
R4212 |
T9259 |
T9258 |
cc |
and,results |
R4213 |
T9260 |
T9261 |
det |
the,findings |
R4214 |
T9261 |
T9258 |
conj |
findings,results |
R4215 |
T9262 |
T9261 |
acl |
obtained,findings |
R4216 |
T9263 |
T9262 |
prep |
from,obtained |
R4217 |
T9264 |
T9265 |
advmod |
in,vitro |
R4218 |
T9265 |
T9266 |
amod |
vitro,experiments |
R4219 |
T9266 |
T9263 |
pobj |
experiments,from |
R4220 |
T9267 |
T9266 |
compound |
culture,experiments |
R4221 |
T9268 |
T9269 |
punct |
(,see |
R4222 |
T9269 |
T9262 |
parataxis |
see,obtained |
R4223 |
T9270 |
T9271 |
nmod |
Figures,4 |
R4224 |
T9271 |
T9269 |
dobj |
4,see |
R4225 |
T9272 |
T9271 |
cc |
and,4 |
R4226 |
T9273 |
T9271 |
conj |
5,4 |
R4227 |
T9274 |
T9269 |
punct |
),see |
R4228 |
T9275 |
T9276 |
mark |
that,leads |
R4229 |
T9276 |
T9255 |
ccomp |
leads,demonstrate |
R4230 |
T9277 |
T9278 |
amod |
precocious,expression |
R4231 |
T9278 |
T9276 |
nsubj |
expression,leads |
R4232 |
T9279 |
T9277 |
cc |
or,precocious |
R4233 |
T9280 |
T9277 |
conj |
isochronic,precocious |
R4234 |
T9281 |
T9278 |
prep |
of,expression |
R4235 |
T9282 |
T9283 |
compound |
EWS,Pea3 |
R4236 |
T9283 |
T9281 |
pobj |
Pea3,of |
R4237 |
T9284 |
T9283 |
punct |
-,Pea3 |
R4238 |
T9285 |
T9278 |
prep |
in,expression |
R4239 |
T9286 |
T9287 |
det |
the,neurons |
R4240 |
T9287 |
T9285 |
pobj |
neurons,in |
R4241 |
T9288 |
T9287 |
amod |
same,neurons |
R4242 |
T9289 |
T9276 |
prep |
to,leads |
R4243 |
T9290 |
T9291 |
advmod |
significantly,different |
R4244 |
T9291 |
T9292 |
amod |
different,responses |
R4245 |
T9292 |
T9289 |
pobj |
responses,to |
R4246 |
T9293 |
T9294 |
npadvmod |
cell,autonomous |
R4247 |
T9294 |
T9292 |
amod |
autonomous,responses |
R4248 |
T9295 |
T9294 |
punct |
-,autonomous |
R4249 |
T9296 |
T9292 |
amod |
cellular,responses |
R4250 |
T9297 |
T9276 |
prep |
with,leads |
R4251 |
T9298 |
T9297 |
pobj |
respect,with |
R4252 |
T9299 |
T9298 |
prep |
to,respect |
R4253 |
T9300 |
T9301 |
compound |
gene,expression |
R4254 |
T9301 |
T9299 |
pobj |
expression,to |
R4255 |
T9302 |
T9301 |
punct |
", ",expression |
R4256 |
T9303 |
T9304 |
amod |
neuronal,survival |
R4257 |
T9304 |
T9301 |
conj |
survival,expression |
R4258 |
T9305 |
T9304 |
punct |
", ",survival |
R4259 |
T9306 |
T9304 |
cc |
and,survival |
R4260 |
T9307 |
T9308 |
compound |
neurite,outgrowth |
R4261 |
T9308 |
T9304 |
conj |
outgrowth,survival |
R4262 |
T9309 |
T9310 |
punct |
(,Figure |
R4263 |
T9310 |
T9255 |
parataxis |
Figure,demonstrate |
R4264 |
T9311 |
T9310 |
nummod |
9,Figure |
R4265 |
T9312 |
T9310 |
punct |
),Figure |
R4266 |
T9313 |
T9255 |
punct |
.,demonstrate |
R4279 |
T10766 |
T10767 |
npadvmod |
Target,derived |
R4280 |
T10767 |
T10769 |
amod |
derived,signals |
R4281 |
T10768 |
T10767 |
punct |
-,derived |
R4282 |
T10769 |
T10770 |
nsubj |
signals,exhibit |
R4283 |
T10771 |
T10772 |
det |
a,role |
R4284 |
T10772 |
T10770 |
dobj |
role,exhibit |
R4285 |
T10773 |
T10772 |
amod |
conserved,role |
R4286 |
T10774 |
T10772 |
prep |
in,role |
R4287 |
T10775 |
T10776 |
det |
the,induction |
R4288 |
T10776 |
T10774 |
pobj |
induction,in |
R4289 |
T10777 |
T10776 |
prep |
of,induction |
R4290 |
T10778 |
T10779 |
amod |
defined,programs |
R4291 |
T10779 |
T10777 |
pobj |
programs,of |
R4292 |
T10780 |
T10779 |
prep |
of,programs |
R4293 |
T10781 |
T10782 |
compound |
transcription,factor |
R4294 |
T10782 |
T10783 |
compound |
factor,expression |
R4295 |
T10783 |
T10780 |
pobj |
expression,of |
R4296 |
T10784 |
T10776 |
amod |
late,induction |
R4297 |
T10785 |
T10784 |
prep |
in,late |
R4298 |
T10786 |
T10787 |
amod |
post-mitotic,differentiation |
R4299 |
T10787 |
T10785 |
pobj |
differentiation,in |
R4300 |
T10788 |
T10787 |
amod |
neuronal,differentiation |
R4301 |
T10789 |
T10790 |
punct |
[,10 |
R4302 |
T10790 |
T10770 |
parataxis |
10,exhibit |
R4303 |
T10791 |
T10790 |
punct |
],10 |
R4304 |
T10792 |
T10770 |
punct |
.,exhibit |
R4305 |
T10794 |
T10795 |
det |
This,study |
R4306 |
T10795 |
T10796 |
nsubj |
study,provides |
R4307 |
T10797 |
T10796 |
dobj |
evidence,provides |
R4308 |
T10798 |
T10799 |
mark |
that,is |
R4309 |
T10799 |
T10797 |
acl |
is,evidence |
R4310 |
T10800 |
T10801 |
det |
the,onset |
R4311 |
T10801 |
T10799 |
nsubj |
onset,is |
R4312 |
T10802 |
T10801 |
amod |
late,onset |
R4313 |
T10803 |
T10801 |
prep |
of,onset |
R4314 |
T10804 |
T10805 |
compound |
transcription,factor |
R4315 |
T10805 |
T10806 |
compound |
factor,expression |
R4316 |
T10806 |
T10803 |
pobj |
expression,of |
R4317 |
T10807 |
T10799 |
acomp |
essential,is |
R4318 |
T10808 |
T10807 |
prep |
for,essential |
R4319 |
T10809 |
T10810 |
amod |
many,aspects |
R4320 |
T10810 |
T10808 |
pobj |
aspects,for |
R4321 |
T10811 |
T10810 |
amod |
later,aspects |
R4322 |
T10812 |
T10810 |
prep |
of,aspects |
R4323 |
T10813 |
T10814 |
amod |
neuronal,differentiation |
R4324 |
T10814 |
T10812 |
pobj |
differentiation,of |
R4325 |
T10815 |
T10814 |
cc |
and,differentiation |
R4326 |
T10816 |
T10817 |
compound |
circuit,formation |
R4327 |
T10817 |
T10814 |
conj |
formation,differentiation |
R4328 |
T10818 |
T10796 |
punct |
.,provides |
R4329 |
T10820 |
T10821 |
poss |
Our,data |
R4330 |
T10821 |
T10822 |
nsubj |
data,indicate |
R4331 |
T10823 |
T10824 |
mark |
that,undergo |
R4332 |
T10824 |
T10822 |
ccomp |
undergo,indicate |
R4333 |
T10825 |
T10826 |
compound |
DRG,neurons |
R4334 |
T10826 |
T10824 |
nsubj |
neurons,undergo |
R4335 |
T10827 |
T10828 |
det |
a,change |
R4336 |
T10828 |
T10824 |
dobj |
change,undergo |
R4337 |
T10829 |
T10828 |
amod |
temporal,change |
R4338 |
T10830 |
T10828 |
prep |
in,change |
R4339 |
T10831 |
T10832 |
poss |
their,competence |
R4340 |
T10832 |
T10830 |
pobj |
competence,in |
R4341 |
T10833 |
T10834 |
aux |
to,respond |
R4342 |
T10834 |
T10824 |
advcl |
respond,undergo |
R4343 |
T10835 |
T10834 |
prep |
to,respond |
R4344 |
T10836 |
T10837 |
compound |
ETS,signaling |
R4345 |
T10837 |
T10835 |
pobj |
signaling,to |
R4346 |
T10838 |
T10839 |
compound |
transcription,factor |
R4347 |
T10839 |
T10837 |
compound |
factor,signaling |
R4348 |
T10840 |
T10824 |
punct |
", ",undergo |
R4349 |
T10841 |
T10842 |
mark |
as,assessed |
R4350 |
T10842 |
T10824 |
advcl |
assessed,undergo |
R4351 |
T10843 |
T10842 |
prep |
by,assessed |
R4352 |
T10844 |
T10843 |
pobj |
changes,by |
R4353 |
T10845 |
T10844 |
prep |
in,changes |
R4354 |
T10846 |
T10847 |
compound |
gene,expression |
R4355 |
T10847 |
T10845 |
pobj |
expression,in |
R4356 |
T10848 |
T10847 |
cc |
and,expression |
R4357 |
T10849 |
T10850 |
amod |
axonal,invasion |
R4358 |
T10850 |
T10847 |
conj |
invasion,expression |
R4359 |
T10851 |
T10850 |
compound |
target,invasion |
R4360 |
T10852 |
T10853 |
punct |
(,Figure |
R4361 |
T10853 |
T10824 |
parataxis |
Figure,undergo |
R4362 |
T10854 |
T10853 |
nummod |
9,Figure |
R4363 |
T10855 |
T10853 |
punct |
),Figure |
R4364 |
T10856 |
T10822 |
punct |
.,indicate |
R4365 |
T10858 |
T10859 |
poss |
Our,findings |
R4366 |
T10859 |
T10860 |
nsubj |
findings,argue |
R4367 |
T10861 |
T10860 |
prep |
for,argue |
R4368 |
T10862 |
T10863 |
det |
the,necessity |
R4369 |
T10863 |
T10861 |
pobj |
necessity,for |
R4370 |
T10864 |
T10863 |
prep |
of,necessity |
R4371 |
T10865 |
T10866 |
npadvmod |
target,induced |
R4372 |
T10866 |
T10868 |
amod |
induced,upregulation |
R4373 |
T10867 |
T10866 |
punct |
-,induced |
R4374 |
T10868 |
T10864 |
pobj |
upregulation,of |
R4375 |
T10869 |
T10866 |
punct |
", ",induced |
R4376 |
T10870 |
T10866 |
cc |
and,induced |
R4377 |
T10871 |
T10870 |
advmod |
therefore,and |
R4378 |
T10872 |
T10873 |
advmod |
temporally,controlled |
R4379 |
T10873 |
T10866 |
conj |
controlled,induced |
R4380 |
T10874 |
T10868 |
punct |
", ",upregulation |
R4381 |
T10875 |
T10868 |
prep |
of,upregulation |
R4382 |
T10876 |
T10877 |
compound |
ETS,signaling |
R4383 |
T10877 |
T10875 |
pobj |
signaling,of |
R4384 |
T10878 |
T10879 |
compound |
transcription,factor |
R4385 |
T10879 |
T10877 |
compound |
factor,signaling |
R4386 |
T10880 |
T10860 |
punct |
.,argue |
R4387 |
T10882 |
T10883 |
advmod |
More,generally |
R4388 |
T10883 |
T10884 |
advmod |
generally,suggest |
R4389 |
T10885 |
T10884 |
punct |
", ",suggest |
R4390 |
T10886 |
T10884 |
nsubj |
they,suggest |
R4391 |
T10887 |
T10888 |
mark |
that,represents |
R4392 |
T10888 |
T10884 |
ccomp |
represents,suggest |
R4393 |
T10889 |
T10890 |
advmod |
temporally,regulated |
R4394 |
T10890 |
T10891 |
amod |
regulated,activation |
R4395 |
T10891 |
T10888 |
nsubj |
activation,represents |
R4396 |
T10892 |
T10891 |
prep |
of,activation |
R4397 |
T10893 |
T10894 |
amod |
transcriptional,programs |
R4398 |
T10894 |
T10892 |
pobj |
programs,of |
R4399 |
T10895 |
T10891 |
acl |
coupled,activation |
R4400 |
T10896 |
T10895 |
prep |
to,coupled |
R4401 |
T10897 |
T10898 |
det |
a,fate |
R4402 |
T10898 |
T10896 |
pobj |
fate,to |
R4403 |
T10899 |
T10898 |
amod |
particular,fate |
R4404 |
T10900 |
T10898 |
acl |
induced,fate |
R4405 |
T10901 |
T10900 |
prep |
in,induced |
R4406 |
T10902 |
T10901 |
pobj |
neurons,in |
R4407 |
T10903 |
T10900 |
prep |
at,induced |
R4408 |
T10904 |
T10905 |
amod |
early,stages |
R4409 |
T10905 |
T10903 |
pobj |
stages,at |
R4410 |
T10906 |
T10905 |
amod |
developmental,stages |
R4411 |
T10907 |
T10908 |
det |
an,mechanism |
R4412 |
T10908 |
T10888 |
dobj |
mechanism,represents |
R4413 |
T10909 |
T10908 |
amod |
important,mechanism |
R4414 |
T10910 |
T10908 |
prep |
of,mechanism |
R4415 |
T10911 |
T10912 |
amod |
neuronal,maturation |
R4416 |
T10912 |
T10910 |
pobj |
maturation,of |
R4417 |
T10913 |
T10884 |
punct |
.,suggest |
R4418 |
T10915 |
T10916 |
nummod |
One,observation |
R4419 |
T10916 |
T10918 |
nsubj |
observation,is |
R4420 |
T10917 |
T10916 |
amod |
striking,observation |
R4421 |
T10919 |
T10916 |
prep |
of,observation |
R4422 |
T10920 |
T10921 |
det |
this,study |
R4423 |
T10921 |
T10919 |
pobj |
study,of |
R4424 |
T10922 |
T10923 |
mark |
that,promotes |
R4425 |
T10923 |
T10918 |
ccomp |
promotes,is |
R4426 |
T10924 |
T10925 |
amod |
precocious,induction |
R4427 |
T10925 |
T10923 |
nsubj |
induction,promotes |
R4428 |
T10926 |
T10925 |
prep |
of,induction |
R4429 |
T10927 |
T10928 |
compound |
ETS,signaling |
R4430 |
T10928 |
T10926 |
pobj |
signaling,of |
R4431 |
T10929 |
T10930 |
amod |
neuronal,survival |
R4432 |
T10930 |
T10923 |
dobj |
survival,promotes |
R4433 |
T10931 |
T10923 |
prep |
without,promotes |
R4434 |
T10932 |
T10933 |
det |
a,requirement |
R4435 |
T10933 |
T10931 |
pobj |
requirement,without |
R4436 |
T10934 |
T10933 |
prep |
for,requirement |
R4437 |
T10935 |
T10936 |
amod |
neurotrophic,support |
R4438 |
T10936 |
T10934 |
pobj |
support,for |
R4439 |
T10937 |
T10931 |
cc |
and,without |
R4440 |
T10938 |
T10931 |
conj |
in,without |
R4441 |
T10939 |
T10940 |
amod |
complete,absence |
R4442 |
T10940 |
T10938 |
pobj |
absence,in |
R4443 |
T10941 |
T10940 |
prep |
of,absence |
R4444 |
T10942 |
T10943 |
compound |
Trk,receptor |
R4445 |
T10943 |
T10944 |
compound |
receptor,expression |
R4446 |
T10944 |
T10941 |
pobj |
expression,of |
R4447 |
T10945 |
T10918 |
punct |
.,is |
R4448 |
T10947 |
T10948 |
prep |
In,result |
R4449 |
T10949 |
T10947 |
pobj |
contrast,In |
R4450 |
T10950 |
T10948 |
punct |
", ",result |
R4451 |
T10951 |
T10952 |
compound |
ETS,signaling |
R4452 |
T10952 |
T10948 |
nsubj |
signaling,result |
R4453 |
T10953 |
T10952 |
prep |
at,signaling |
R4454 |
T10954 |
T10955 |
det |
the,time |
R4455 |
T10955 |
T10953 |
pobj |
time,at |
R4456 |
T10956 |
T10955 |
amod |
normal,time |
R4457 |
T10957 |
T10955 |
prep |
of,time |
R4458 |
T10958 |
T10957 |
pobj |
onset,of |
R4459 |
T10959 |
T10958 |
prep |
of,onset |
R4460 |
T10960 |
T10961 |
compound |
Er81,expression |
R4461 |
T10961 |
T10959 |
pobj |
expression,of |
R4462 |
T10962 |
T10948 |
aux |
does,result |
R4463 |
T10963 |
T10948 |
neg |
not,result |
R4464 |
T10964 |
T10948 |
prep |
in,result |
R4465 |
T10965 |
T10966 |
amod |
enhanced,survival |
R4466 |
T10966 |
T10964 |
pobj |
survival,in |
R4467 |
T10967 |
T10966 |
amod |
neuronal,survival |
R4468 |
T10968 |
T10948 |
prep |
in,result |
R4469 |
T10969 |
T10970 |
det |
the,absence |
R4470 |
T10970 |
T10968 |
pobj |
absence,in |
R4471 |
T10971 |
T10970 |
prep |
of,absence |
R4472 |
T10972 |
T10971 |
pobj |
neurotrophins,of |
R4473 |
T10973 |
T10948 |
cc |
and,result |
R4474 |
T10974 |
T10975 |
advmod |
also,lead |
R4475 |
T10975 |
T10948 |
conj |
lead,result |
R4476 |
T10976 |
T10975 |
aux |
does,lead |
R4477 |
T10977 |
T10975 |
neg |
not,lead |
R4478 |
T10978 |
T10975 |
prep |
to,lead |
R4479 |
T10979 |
T10978 |
pobj |
downregulation,to |
R4480 |
T10980 |
T10979 |
prep |
of,downregulation |
R4481 |
T10981 |
T10982 |
compound |
TrkC,expression |
R4482 |
T10982 |
T10980 |
pobj |
expression,of |
R4483 |
T10983 |
T10979 |
prep |
in,downregulation |
R4484 |
T10984 |
T10985 |
amod |
proprioceptive,afferents |
R4485 |
T10985 |
T10983 |
pobj |
afferents,in |
R4486 |
T10986 |
T10948 |
punct |
.,result |
R4487 |
T10988 |
T10989 |
det |
These,findings |
R4488 |
T10989 |
T10990 |
nsubj |
findings,demonstrate |
R4489 |
T10991 |
T10992 |
advmod |
very,distinct |
R4490 |
T10992 |
T10993 |
amod |
distinct,activities |
R4491 |
T10993 |
T10990 |
dobj |
activities,demonstrate |
R4492 |
T10994 |
T10993 |
prep |
of,activities |
R4493 |
T10995 |
T10996 |
nummod |
one,regulator |
R4494 |
T10996 |
T10994 |
pobj |
regulator,of |
R4495 |
T10997 |
T10996 |
amod |
transcriptional,regulator |
R4496 |
T10998 |
T10990 |
prep |
at,demonstrate |
R4497 |
T10999 |
T11000 |
amod |
different,steps |
R4498 |
T11000 |
T10998 |
pobj |
steps,at |
R4499 |
T11001 |
T11000 |
amod |
developmental,steps |
R4500 |
T11002 |
T11000 |
prep |
within,steps |
R4501 |
T11003 |
T11004 |
det |
a,lineage |
R4502 |
T11004 |
T11002 |
pobj |
lineage,within |
R4503 |
T11005 |
T11004 |
amod |
committed,lineage |
R4504 |
T11006 |
T11004 |
amod |
post-mitotic,lineage |
R4505 |
T11007 |
T11004 |
amod |
neuronal,lineage |
R4506 |
T11008 |
T10990 |
punct |
.,demonstrate |
R4507 |
T11010 |
T11011 |
det |
The,absence |
R4508 |
T11011 |
T11012 |
nsubj |
absence,explain |
R4509 |
T11013 |
T11011 |
prep |
of,absence |
R4510 |
T11014 |
T11015 |
compound |
Trk,expression |
R4511 |
T11015 |
T11013 |
pobj |
expression,of |
R4512 |
T11016 |
T11015 |
compound |
receptor,expression |
R4513 |
T11017 |
T11011 |
prep |
upon,absence |
R4514 |
T11018 |
T11019 |
amod |
precocious,induction |
R4515 |
T11019 |
T11017 |
pobj |
induction,upon |
R4516 |
T11020 |
T11019 |
prep |
of,induction |
R4517 |
T11021 |
T11022 |
compound |
ETS,signaling |
R4518 |
T11022 |
T11020 |
pobj |
signaling,of |
R4519 |
T11023 |
T11012 |
aux |
can,explain |
R4520 |
T11024 |
T11025 |
advmod |
only,partially |
R4521 |
T11025 |
T11012 |
advmod |
partially,explain |
R4522 |
T11026 |
T11027 |
det |
the,phenotype |
R4523 |
T11027 |
T11012 |
dobj |
phenotype,explain |
R4524 |
T11028 |
T11027 |
amod |
observed,phenotype |
R4525 |
T11029 |
T11012 |
prep |
in,explain |
R4526 |
T11030 |
T11031 |
amod |
axonal,projections |
R4527 |
T11031 |
T11029 |
pobj |
projections,in |
R4528 |
T11032 |
T11012 |
punct |
.,explain |
R4529 |
T11034 |
T11035 |
nsubj |
Elimination,perturbs |
R4530 |
T11036 |
T11034 |
prep |
of,Elimination |
R4531 |
T11037 |
T11038 |
compound |
TrkA,receptor |
R4532 |
T11038 |
T11039 |
compound |
receptor,signaling |
R4533 |
T11039 |
T11036 |
pobj |
signaling,of |
R4534 |
T11040 |
T11034 |
prep |
in,Elimination |
R4535 |
T11041 |
T11042 |
compound |
Bax,mice |
R4536 |
T11042 |
T11040 |
pobj |
mice,in |
R4537 |
T11043 |
T11042 |
compound |
mutant,mice |
R4538 |
T11044 |
T11035 |
dobj |
establishment,perturbs |
R4539 |
T11045 |
T11044 |
prep |
of,establishment |
R4540 |
T11046 |
T11047 |
amod |
peripheral,projections |
R4541 |
T11047 |
T11045 |
pobj |
projections,of |
R4542 |
T11048 |
T11047 |
prep |
of,projections |
R4543 |
T11049 |
T11050 |
amod |
cutaneous,afferents |
R4544 |
T11050 |
T11048 |
pobj |
afferents,of |
R4545 |
T11051 |
T11035 |
punct |
", ",perturbs |
R4546 |
T11052 |
T11053 |
mark |
whereas,appear |
R4547 |
T11053 |
T11035 |
advcl |
appear,perturbs |
R4548 |
T11054 |
T11053 |
nsubj |
establishment,appear |
R4549 |
T11055 |
T11054 |
prep |
of,establishment |
R4550 |
T11056 |
T11057 |
amod |
central,projections |
R4551 |
T11057 |
T11055 |
pobj |
projections,of |
R4552 |
T11058 |
T11053 |
aux |
does,appear |
R4553 |
T11059 |
T11053 |
neg |
not,appear |
R4554 |
T11060 |
T11061 |
aux |
to,affected |
R4555 |
T11061 |
T11053 |
xcomp |
affected,appear |
R4556 |
T11062 |
T11061 |
auxpass |
be,affected |
R4557 |
T11063 |
T11064 |
punct |
[,8 |
R4558 |
T11064 |
T11053 |
parataxis |
8,appear |
R4559 |
T11065 |
T11064 |
punct |
],8 |
R4560 |
T11066 |
T11035 |
punct |
.,perturbs |
R4561 |
T11068 |
T11069 |
prep |
In,perturbed |
R4562 |
T11070 |
T11071 |
det |
the,absence |
R4563 |
T11071 |
T11068 |
pobj |
absence,In |
R4564 |
T11072 |
T11071 |
prep |
of,absence |
R4565 |
T11073 |
T11074 |
nmod |
NT,signaling |
R4566 |
T11074 |
T11072 |
pobj |
signaling,of |
R4567 |
T11075 |
T11073 |
punct |
-,NT |
R4568 |
T11076 |
T11073 |
nummod |
3,NT |
R4569 |
T11077 |
T11069 |
punct |
", ",perturbed |
R4570 |
T11078 |
T11069 |
nsubjpass |
development,perturbed |
R4571 |
T11079 |
T11078 |
prep |
of,development |
R4572 |
T11080 |
T11081 |
amod |
central,projections |
R4573 |
T11081 |
T11079 |
pobj |
projections,of |
R4574 |
T11082 |
T11083 |
advmod |
as,as |
R4575 |
T11083 |
T11080 |
cc |
as,central |
R4576 |
T11084 |
T11083 |
advmod |
well,as |
R4577 |
T11085 |
T11080 |
conj |
peripheral,central |
R4578 |
T11086 |
T11081 |
amod |
proprioceptive,projections |
R4579 |
T11087 |
T11081 |
amod |
afferent,projections |
R4580 |
T11088 |
T11069 |
auxpass |
is,perturbed |
R4581 |
T11089 |
T11090 |
punct |
[,9 |
R4582 |
T11090 |
T11069 |
parataxis |
9,perturbed |
R4583 |
T11091 |
T11090 |
punct |
],9 |
R4584 |
T11092 |
T11069 |
punct |
.,perturbed |
R4585 |
T11094 |
T11095 |
prep |
In,found |
R4586 |
T11096 |
T11094 |
pobj |
contrast,In |
R4587 |
T11097 |
T11095 |
punct |
", ",found |
R4588 |
T11098 |
T11095 |
prep |
upon,found |
R4589 |
T11099 |
T11100 |
amod |
precocious,induction |
R4590 |
T11100 |
T11098 |
pobj |
induction,upon |
R4591 |
T11101 |
T11100 |
prep |
of,induction |
R4592 |
T11102 |
T11103 |
compound |
ETS,signaling |
R4593 |
T11103 |
T11101 |
pobj |
signaling,of |
R4594 |
T11104 |
T11095 |
punct |
", ",found |
R4595 |
T11105 |
T11095 |
nsubj |
we,found |
R4596 |
T11106 |
T11107 |
advmod |
more,pronounced |
R4597 |
T11107 |
T11108 |
amod |
pronounced,defects |
R4598 |
T11108 |
T11095 |
dobj |
defects,found |
R4599 |
T11109 |
T11095 |
prep |
in,found |
R4600 |
T11110 |
T11111 |
det |
the,establishment |
R4601 |
T11111 |
T11109 |
pobj |
establishment,in |
R4602 |
T11112 |
T11111 |
prep |
of,establishment |
R4603 |
T11113 |
T11114 |
amod |
central,projections |
R4604 |
T11114 |
T11112 |
pobj |
projections,of |
R4605 |
T11115 |
T11116 |
advmod |
rather,than |
R4606 |
T11116 |
T11113 |
cc |
than,central |
R4607 |
T11117 |
T11113 |
conj |
peripheral,central |
R4608 |
T11118 |
T11111 |
prep |
for,establishment |
R4609 |
T11119 |
T11120 |
det |
all,neurons |
R4610 |
T11120 |
T11118 |
pobj |
neurons,for |
R4611 |
T11121 |
T11120 |
compound |
DRG,neurons |
R4612 |
T11122 |
T11095 |
punct |
.,found |
R4613 |
T11124 |
T11125 |
nsubjpass |
Induction,controlled |
R4614 |
T11126 |
T11124 |
prep |
of,Induction |
R4615 |
T11127 |
T11128 |
compound |
Er81,expression |
R4616 |
T11128 |
T11126 |
pobj |
expression,of |
R4617 |
T11129 |
T11124 |
prep |
in,Induction |
R4618 |
T11130 |
T11131 |
amod |
proprioceptive,afferents |
R4619 |
T11131 |
T11129 |
pobj |
afferents,in |
R4620 |
T11132 |
T11125 |
auxpass |
is,controlled |
R4621 |
T11133 |
T11125 |
agent |
by,controlled |
R4622 |
T11134 |
T11135 |
amod |
peripheral,NT |
R4623 |
T11135 |
T11133 |
pobj |
NT,by |
R4624 |
T11136 |
T11135 |
punct |
-,NT |
R4625 |
T11137 |
T11135 |
nummod |
3,NT |
R4626 |
T11138 |
T11139 |
mark |
as,reach |
R4627 |
T11139 |
T11125 |
advcl |
reach,controlled |
R4628 |
T11140 |
T11139 |
nsubj |
axons,reach |
R4629 |
T11141 |
T11142 |
det |
the,vicinity |
R4630 |
T11142 |
T11139 |
dobj |
vicinity,reach |
R4631 |
T11143 |
T11142 |
prep |
of,vicinity |
R4632 |
T11144 |
T11145 |
compound |
target,muscles |
R4633 |
T11145 |
T11143 |
pobj |
muscles,of |
R4634 |
T11146 |
T11125 |
punct |
", ",controlled |
R4635 |
T11147 |
T11125 |
cc |
and,controlled |
R4636 |
T11148 |
T11149 |
advmod |
thus,occurs |
R4637 |
T11149 |
T11125 |
conj |
occurs,controlled |
R4638 |
T11150 |
T11151 |
advmod |
only,d |
R4639 |
T11151 |
T11154 |
npadvmod |
d,become |
R4640 |
T11152 |
T11153 |
advmod |
approximately,3 |
R4641 |
T11153 |
T11151 |
nummod |
3,d |
R4642 |
T11154 |
T11149 |
advcl |
become,occurs |
R4643 |
T11155 |
T11154 |
mark |
after,become |
R4644 |
T11156 |
T11157 |
amod |
proprioceptive,neurons |
R4645 |
T11157 |
T11154 |
nsubj |
neurons,become |
R4646 |
T11158 |
T11154 |
acomp |
post-mitotic,become |
R4647 |
T11159 |
T11160 |
punct |
[,14 |
R4648 |
T11160 |
T11154 |
parataxis |
14,become |
R4649 |
T11161 |
T11160 |
nummod |
9,14 |
R4650 |
T11162 |
T11160 |
punct |
",",14 |
R4651 |
T11163 |
T11160 |
punct |
],14 |
R4652 |
T11164 |
T11125 |
punct |
.,controlled |
R4653 |
T11166 |
T11167 |
det |
This,upregulation |
R4654 |
T11167 |
T11174 |
nsubjpass |
upregulation,restricted |
R4655 |
T11168 |
T11169 |
advmod |
temporally,delayed |
R4656 |
T11169 |
T11167 |
amod |
delayed,upregulation |
R4657 |
T11170 |
T11169 |
cc |
and,delayed |
R4658 |
T11171 |
T11172 |
npadvmod |
target,induced |
R4659 |
T11172 |
T11169 |
conj |
induced,delayed |
R4660 |
T11173 |
T11172 |
punct |
-,induced |
R4661 |
T11175 |
T11167 |
prep |
of,upregulation |
R4662 |
T11176 |
T11177 |
compound |
ETS,expression |
R4663 |
T11177 |
T11175 |
pobj |
expression,of |
R4664 |
T11178 |
T11179 |
compound |
transcription,factor |
R4665 |
T11179 |
T11177 |
compound |
factor,expression |
R4666 |
T11180 |
T11181 |
amod |
several,days |
R4667 |
T11181 |
T11182 |
npadvmod |
days,emerges |
R4668 |
T11182 |
T11167 |
advcl |
emerges,upregulation |
R4669 |
T11183 |
T11182 |
mark |
after,emerges |
R4670 |
T11184 |
T11185 |
det |
a,lineage |
R4671 |
T11185 |
T11182 |
nsubj |
lineage,emerges |
R4672 |
T11186 |
T11185 |
amod |
neuronal,lineage |
R4673 |
T11187 |
T11185 |
prep |
of,lineage |
R4674 |
T11188 |
T11189 |
det |
a,identity |
R4675 |
T11189 |
T11187 |
pobj |
identity,of |
R4676 |
T11190 |
T11189 |
amod |
specific,identity |
R4677 |
T11191 |
T11182 |
advmod |
first,emerges |
R4678 |
T11192 |
T11174 |
auxpass |
is,restricted |
R4679 |
T11193 |
T11174 |
neg |
not,restricted |
R4680 |
T11194 |
T11174 |
prep |
to,restricted |
R4681 |
T11195 |
T11196 |
nmod |
DRG,neurons |
R4682 |
T11196 |
T11194 |
pobj |
neurons,to |
R4683 |
T11197 |
T11196 |
amod |
sensory,neurons |
R4684 |
T11198 |
T11174 |
punct |
", ",restricted |
R4685 |
T11199 |
T11174 |
cc |
but,restricted |
R4686 |
T11200 |
T11201 |
auxpass |
is,found |
R4687 |
T11201 |
T11174 |
conj |
found,restricted |
R4688 |
T11202 |
T11201 |
advmod |
also,found |
R4689 |
T11203 |
T11201 |
prep |
in,found |
R4690 |
T11204 |
T11205 |
compound |
motor,neuron |
R4691 |
T11205 |
T11206 |
compound |
neuron,pools |
R4692 |
T11206 |
T11203 |
pobj |
pools,in |
R4693 |
T11207 |
T11208 |
punct |
[,13 |
R4694 |
T11208 |
T11201 |
parataxis |
13,found |
R4695 |
T11209 |
T11208 |
punct |
],13 |
R4696 |
T11210 |
T11174 |
punct |
.,restricted |
R4697 |
T11212 |
T11213 |
npadvmod |
Target,derived |
R4698 |
T11213 |
T11215 |
amod |
derived,factors |
R4699 |
T11214 |
T11213 |
punct |
-,derived |
R4700 |
T11215 |
T11216 |
nsubjpass |
factors,implicated |
R4701 |
T11217 |
T11216 |
aux |
have,implicated |
R4702 |
T11218 |
T11216 |
advmod |
also,implicated |
R4703 |
T11219 |
T11216 |
auxpass |
been,implicated |
R4704 |
T11220 |
T11216 |
prep |
in,implicated |
R4705 |
T11221 |
T11220 |
pcomp |
controlling,in |
R4706 |
T11222 |
T11223 |
amod |
neuronal,maturation |
R4707 |
T11223 |
T11221 |
dobj |
maturation,controlling |
R4708 |
T11224 |
T11223 |
prep |
of,maturation |
R4709 |
T11225 |
T11226 |
amod |
predetermined,neurons |
R4710 |
T11226 |
T11224 |
pobj |
neurons,of |
R4711 |
T11227 |
T11221 |
prep |
in,controlling |
R4712 |
T11228 |
T11227 |
pobj |
Drosophila,in |
R4713 |
T11229 |
T11228 |
punct |
", ",Drosophila |
R4714 |
T11230 |
T11231 |
prep |
in,is |
R4715 |
T11231 |
T11228 |
relcl |
is,Drosophila |
R4716 |
T11232 |
T11230 |
pobj |
which,in |
R4717 |
T11233 |
T11231 |
nsubj |
expression,is |
R4718 |
T11234 |
T11233 |
prep |
of,expression |
R4719 |
T11235 |
T11234 |
pobj |
members,of |
R4720 |
T11236 |
T11235 |
prep |
of,members |
R4721 |
T11237 |
T11238 |
det |
the,family |
R4722 |
T11238 |
T11236 |
pobj |
family,of |
R4723 |
T11239 |
T11238 |
compound |
BMP,family |
R4724 |
T11240 |
T11233 |
prep |
in,expression |
R4725 |
T11241 |
T11242 |
det |
the,region |
R4726 |
T11242 |
T11240 |
pobj |
region,in |
R4727 |
T11243 |
T11242 |
compound |
target,region |
R4728 |
T11244 |
T11231 |
acomp |
essential,is |
R4729 |
T11245 |
T11244 |
prep |
for,essential |
R4730 |
T11246 |
T11247 |
det |
the,induction |
R4731 |
T11247 |
T11245 |
pobj |
induction,for |
R4732 |
T11248 |
T11247 |
prep |
of,induction |
R4733 |
T11249 |
T11250 |
amod |
mature,properties |
R4734 |
T11250 |
T11248 |
pobj |
properties,of |
R4735 |
T11251 |
T11250 |
amod |
peptidergic,properties |
R4736 |
T11252 |
T11231 |
prep |
in,is |
R4737 |
T11253 |
T11254 |
det |
a,subpopulation |
R4738 |
T11254 |
T11252 |
pobj |
subpopulation,in |
R4739 |
T11255 |
T11254 |
prep |
of,subpopulation |
R4740 |
T11256 |
T11255 |
pobj |
neurons,of |
R4741 |
T11257 |
T11254 |
acl |
marked,subpopulation |
R4742 |
T11258 |
T11257 |
agent |
by,marked |
R4743 |
T11259 |
T11260 |
det |
the,expression |
R4744 |
T11260 |
T11258 |
pobj |
expression,by |
R4745 |
T11261 |
T11260 |
compound |
coordinate,expression |
R4746 |
T11262 |
T11260 |
prep |
of,expression |
R4747 |
T11263 |
T11264 |
det |
the,factors |
R4748 |
T11264 |
T11262 |
pobj |
factors,of |
R4749 |
T11265 |
T11264 |
nummod |
two,factors |
R4750 |
T11266 |
T11264 |
compound |
transcription,factors |
R4751 |
T11267 |
T11264 |
appos |
Apterous,factors |
R4752 |
T11268 |
T11267 |
cc |
and,Apterous |
R4753 |
T11269 |
T11267 |
conj |
Squeeze,Apterous |
R4754 |
T11270 |
T11271 |
punct |
[,12 |
R4755 |
T11271 |
T11257 |
parataxis |
12,marked |
R4756 |
T11272 |
T11271 |
nummod |
11,12 |
R4757 |
T11273 |
T11271 |
punct |
",",12 |
R4758 |
T11274 |
T11271 |
punct |
],12 |
R4759 |
T11275 |
T11216 |
punct |
.,implicated |
R4760 |
T11277 |
T11278 |
advmod |
Thus,appear |
R4761 |
T11279 |
T11278 |
punct |
", ",appear |
R4762 |
T11280 |
T11281 |
preconj |
both,in |
R4763 |
T11281 |
T11278 |
prep |
in,appear |
R4764 |
T11282 |
T11281 |
pobj |
Drosophila,in |
R4765 |
T11283 |
T11282 |
cc |
and,Drosophila |
R4766 |
T11284 |
T11282 |
conj |
vertebrates,Drosophila |
R4767 |
T11285 |
T11278 |
punct |
", ",appear |
R4768 |
T11286 |
T11287 |
npadvmod |
target,derived |
R4769 |
T11287 |
T11289 |
amod |
derived,factors |
R4770 |
T11288 |
T11287 |
punct |
-,derived |
R4771 |
T11289 |
T11278 |
nsubj |
factors,appear |
R4772 |
T11290 |
T11291 |
aux |
to,act |
R4773 |
T11291 |
T11278 |
xcomp |
act,appear |
R4774 |
T11292 |
T11291 |
advmod |
permissively,act |
R4775 |
T11293 |
T11294 |
aux |
to,induce |
R4776 |
T11294 |
T11291 |
advcl |
induce,act |
R4777 |
T11295 |
T11296 |
det |
the,expression |
R4778 |
T11296 |
T11294 |
dobj |
expression,induce |
R4779 |
T11297 |
T11296 |
prep |
of,expression |
R4780 |
T11298 |
T11299 |
amod |
transcriptional,programs |
R4781 |
T11299 |
T11297 |
pobj |
programs,of |
R4782 |
T11300 |
T11299 |
acl |
involved,programs |
R4783 |
T11301 |
T11300 |
prep |
in,involved |
R4784 |
T11302 |
T11303 |
amod |
terminal,maturation |
R4785 |
T11303 |
T11301 |
pobj |
maturation,in |
R4786 |
T11304 |
T11303 |
amod |
neuronal,maturation |
R4787 |
T11305 |
T11278 |
punct |
.,appear |
R4788 |
T11307 |
T11308 |
poss |
Our,findings |
R4789 |
T11308 |
T11309 |
nsubj |
findings,are |
R4790 |
T11310 |
T11309 |
acomp |
compatible,are |
R4791 |
T11311 |
T11310 |
prep |
with,compatible |
R4792 |
T11312 |
T11313 |
det |
a,model |
R4793 |
T11313 |
T11311 |
pobj |
model,with |
R4794 |
T11314 |
T11315 |
prep |
in,acquire |
R4795 |
T11315 |
T11313 |
relcl |
acquire,model |
R4796 |
T11316 |
T11314 |
pobj |
which,in |
R4797 |
T11317 |
T11318 |
compound |
DRG,neurons |
R4798 |
T11318 |
T11315 |
nsubj |
neurons,acquire |
R4799 |
T11319 |
T11320 |
poss |
their,fate |
R4800 |
T11320 |
T11315 |
dobj |
fate,acquire |
R4801 |
T11321 |
T11320 |
amod |
mature,fate |
R4802 |
T11322 |
T11315 |
prep |
by,acquire |
R4803 |
T11323 |
T11324 |
amod |
sequential,addition |
R4804 |
T11324 |
T11322 |
pobj |
addition,by |
R4805 |
T11325 |
T11323 |
cc |
and,sequential |
R4806 |
T11326 |
T11327 |
advmod |
temporally,controlled |
R4807 |
T11327 |
T11323 |
conj |
controlled,sequential |
R4808 |
T11328 |
T11324 |
prep |
of,addition |
R4809 |
T11329 |
T11330 |
npadvmod |
lineage,specific |
R4810 |
T11330 |
T11332 |
amod |
specific,features |
R4811 |
T11331 |
T11330 |
punct |
-,specific |
R4812 |
T11332 |
T11328 |
pobj |
features,of |
R4813 |
T11333 |
T11334 |
punct |
(,Figure |
R4814 |
T11334 |
T11315 |
parataxis |
Figure,acquire |
R4815 |
T11335 |
T11334 |
nummod |
9,Figure |
R4816 |
T11336 |
T11334 |
punct |
),Figure |
R4817 |
T11337 |
T11309 |
punct |
.,are |
R4818 |
T11339 |
T11340 |
npadvmod |
Target,derived |
R4819 |
T11340 |
T11342 |
amod |
derived,factors |
R4820 |
T11341 |
T11340 |
punct |
-,derived |
R4821 |
T11342 |
T11343 |
nsubj |
factors,act |
R4822 |
T11344 |
T11343 |
prep |
on,act |
R4823 |
T11345 |
T11346 |
amod |
predetermined,lineages |
R4824 |
T11346 |
T11344 |
pobj |
lineages,on |
R4825 |
T11347 |
T11346 |
amod |
neuronal,lineages |
R4826 |
T11348 |
T11349 |
aux |
to,switch |
R4827 |
T11349 |
T11343 |
advcl |
switch,act |
R4828 |
T11350 |
T11351 |
poss |
their,programs |
R4829 |
T11351 |
T11349 |
dobj |
programs,switch |
R4830 |
T11352 |
T11351 |
amod |
developmental,programs |
R4831 |
T11353 |
T11354 |
aux |
to,become |
R4832 |
T11354 |
T11349 |
advcl |
become,switch |
R4833 |
T11355 |
T11354 |
acomp |
compatible,become |
R4834 |
T11356 |
T11355 |
prep |
with,compatible |
R4835 |
T11357 |
T11356 |
pobj |
processes,with |
R4836 |
T11358 |
T11359 |
amod |
such,as |
R4837 |
T11359 |
T11357 |
prep |
as,processes |
R4838 |
T11360 |
T11361 |
compound |
target,invasion |
R4839 |
T11361 |
T11359 |
pobj |
invasion,as |
R4840 |
T11362 |
T11361 |
cc |
and,invasion |
R4841 |
T11363 |
T11361 |
conj |
branching,invasion |
R4842 |
T11364 |
T11343 |
punct |
.,act |
R4843 |
T11366 |
T11367 |
predet |
Such,state |
R4844 |
T11367 |
T11370 |
nsubjpass |
state,accompanied |
R4845 |
T11368 |
T11367 |
det |
a,state |
R4846 |
T11369 |
T11367 |
compound |
transition,state |
R4847 |
T11371 |
T11367 |
prep |
in,state |
R4848 |
T11372 |
T11373 |
det |
the,acquisition |
R4849 |
T11373 |
T11371 |
pobj |
acquisition,in |
R4850 |
T11374 |
T11373 |
prep |
of,acquisition |
R4851 |
T11375 |
T11376 |
det |
a,fate |
R4852 |
T11376 |
T11374 |
pobj |
fate,of |
R4853 |
T11377 |
T11376 |
amod |
defined,fate |
R4854 |
T11378 |
T11376 |
amod |
neuronal,fate |
R4855 |
T11379 |
T11370 |
aux |
would,accompanied |
R4856 |
T11380 |
T11370 |
auxpass |
be,accompanied |
R4857 |
T11381 |
T11370 |
agent |
by,accompanied |
R4858 |
T11382 |
T11383 |
det |
the,induction |
R4859 |
T11383 |
T11381 |
pobj |
induction,by |
R4860 |
T11384 |
T11383 |
prep |
of,induction |
R4861 |
T11385 |
T11386 |
amod |
appropriate,programs |
R4862 |
T11386 |
T11384 |
pobj |
programs,of |
R4863 |
T11387 |
T11386 |
amod |
transcriptional,programs |
R4864 |
T11388 |
T11383 |
prep |
through,induction |
R4865 |
T11389 |
T11390 |
det |
the,expression |
R4866 |
T11390 |
T11388 |
pobj |
expression,through |
R4867 |
T11391 |
T11390 |
prep |
of,expression |
R4868 |
T11392 |
T11393 |
amod |
specific,factors |
R4869 |
T11393 |
T11391 |
pobj |
factors,of |
R4870 |
T11394 |
T11393 |
compound |
transcription,factors |
R4871 |
T11395 |
T11370 |
punct |
.,accompanied |
R4872 |
T11397 |
T11398 |
nsubj |
Mechanisms,represent |
R4873 |
T11399 |
T11400 |
amod |
such,as |
R4874 |
T11400 |
T11397 |
prep |
as,Mechanisms |
R4875 |
T11401 |
T11402 |
amod |
chromosomal,remodeling |
R4876 |
T11402 |
T11400 |
pobj |
remodeling,as |
R4877 |
T11403 |
T11404 |
dep |
that,restrict |
R4878 |
T11404 |
T11397 |
relcl |
restrict,Mechanisms |
R4879 |
T11405 |
T11404 |
cc |
or,restrict |
R4880 |
T11406 |
T11404 |
conj |
expand,restrict |
R4881 |
T11407 |
T11406 |
dobj |
access,expand |
R4882 |
T11408 |
T11407 |
prep |
to,access |
R4883 |
T11409 |
T11410 |
amod |
certain,genes |
R4884 |
T11410 |
T11408 |
pobj |
genes,to |
R4885 |
T11411 |
T11410 |
compound |
target,genes |
R4886 |
T11412 |
T11413 |
punct |
[,34 |
R4887 |
T11413 |
T11407 |
parataxis |
34,access |
R4888 |
T11414 |
T11413 |
punct |
],34 |
R4889 |
T11415 |
T11407 |
cc |
or,access |
R4890 |
T11416 |
T11407 |
conj |
activation,access |
R4891 |
T11417 |
T11416 |
prep |
by,activation |
R4892 |
T11418 |
T11417 |
pobj |
cofactors,by |
R4893 |
T11419 |
T11418 |
amod |
responsible,cofactors |
R4894 |
T11420 |
T11419 |
prep |
for,responsible |
R4895 |
T11421 |
T11420 |
pcomp |
changing,for |
R4896 |
T11422 |
T11423 |
det |
the,action |
R4897 |
T11423 |
T11421 |
dobj |
action,changing |
R4898 |
T11424 |
T11423 |
prep |
of,action |
R4899 |
T11425 |
T11426 |
amod |
particular,factors |
R4900 |
T11426 |
T11424 |
pobj |
factors,of |
R4901 |
T11427 |
T11426 |
compound |
transcription,factors |
R4902 |
T11428 |
T11429 |
punct |
[,35 |
R4903 |
T11429 |
T11406 |
parataxis |
35,expand |
R4904 |
T11430 |
T11429 |
punct |
],35 |
R4905 |
T11431 |
T11398 |
aux |
could,represent |
R4906 |
T11432 |
T11433 |
amod |
possible,mechanisms |
R4907 |
T11433 |
T11398 |
dobj |
mechanisms,represent |
R4908 |
T11434 |
T11435 |
prep |
by,shifted |
R4909 |
T11435 |
T11398 |
ccomp |
shifted,represent |
R4910 |
T11436 |
T11434 |
pobj |
which,by |
R4911 |
T11437 |
T11438 |
det |
the,profile |
R4912 |
T11438 |
T11435 |
nsubjpass |
profile,shifted |
R4913 |
T11439 |
T11438 |
amod |
downstream,profile |
R4914 |
T11440 |
T11438 |
amod |
transcriptional,profile |
R4915 |
T11441 |
T11438 |
prep |
of,profile |
R4916 |
T11442 |
T11443 |
det |
a,factor |
R4917 |
T11443 |
T11441 |
pobj |
factor,of |
R4918 |
T11444 |
T11443 |
compound |
transcription,factor |
R4919 |
T11445 |
T11435 |
aux |
could,shifted |
R4920 |
T11446 |
T11435 |
auxpass |
be,shifted |
R4921 |
T11447 |
T11435 |
advmod |
temporally,shifted |
R4922 |
T11448 |
T11435 |
prep |
towards,shifted |
R4923 |
T11449 |
T11450 |
det |
the,selection |
R4924 |
T11450 |
T11448 |
pobj |
selection,towards |
R4925 |
T11451 |
T11450 |
cc |
and,selection |
R4926 |
T11452 |
T11450 |
conj |
control,selection |
R4927 |
T11453 |
T11450 |
prep |
of,selection |
R4928 |
T11454 |
T11455 |
amod |
distinct,genes |
R4929 |
T11455 |
T11453 |
pobj |
genes,of |
R4930 |
T11456 |
T11455 |
compound |
target,genes |
R4931 |
T11457 |
T11398 |
punct |
.,represent |
R4932 |
T11459 |
T11460 |
advmod |
Interestingly,activated |
R4933 |
T11461 |
T11460 |
punct |
", ",activated |
R4934 |
T11462 |
T11463 |
amod |
several,factors |
R4935 |
T11463 |
T11460 |
nsubjpass |
factors,activated |
R4936 |
T11464 |
T11463 |
compound |
ETS,factors |
R4937 |
T11465 |
T11463 |
compound |
transcription,factors |
R4938 |
T11466 |
T11460 |
auxpass |
are,activated |
R4939 |
T11467 |
T11460 |
prep |
through,activated |
R4940 |
T11468 |
T11467 |
pobj |
release,through |
R4941 |
T11469 |
T11468 |
prep |
of,release |
R4942 |
T11470 |
T11469 |
pobj |
autoinhibition,of |
R4943 |
T11471 |
T11460 |
prep |
via,activated |
R4944 |
T11472 |
T11471 |
pobj |
interaction,via |
R4945 |
T11473 |
T11472 |
prep |
with,interaction |
R4946 |
T11474 |
T11473 |
pobj |
cofactors,with |
R4947 |
T11475 |
T11471 |
cc |
and,via |
R4948 |
T11476 |
T11475 |
punct |
/,and |
R4949 |
T11477 |
T11475 |
cc |
or,and |
R4950 |
T11478 |
T11471 |
conj |
via,via |
R4951 |
T11479 |
T11480 |
amod |
post-translational,modifications |
R4952 |
T11480 |
T11478 |
pobj |
modifications,via |
R4953 |
T11481 |
T11482 |
punct |
[,37 |
R4954 |
T11482 |
T11460 |
parataxis |
37,activated |
R4955 |
T11483 |
T11482 |
nummod |
35,37 |
R4956 |
T11484 |
T11482 |
punct |
",",37 |
R4957 |
T11485 |
T11482 |
nummod |
36,37 |
R4958 |
T11486 |
T11482 |
punct |
",",37 |
R4959 |
T11487 |
T11482 |
punct |
],37 |
R4960 |
T11488 |
T11460 |
punct |
.,activated |
R4961 |
T11490 |
T11491 |
det |
The,fusion |
R4962 |
T11491 |
T11492 |
nsubj |
fusion,circumvent |
R4963 |
T11493 |
T11491 |
prep |
of,fusion |
R4964 |
T11494 |
T11493 |
pobj |
EWS,of |
R4965 |
T11495 |
T11491 |
prep |
with,fusion |
R4966 |
T11496 |
T11495 |
pobj |
Pea3,with |
R4967 |
T11497 |
T11492 |
aux |
could,circumvent |
R4968 |
T11498 |
T11499 |
det |
a,need |
R4969 |
T11499 |
T11492 |
dobj |
need,circumvent |
R4970 |
T11500 |
T11499 |
prep |
for,need |
R4971 |
T11501 |
T11500 |
pobj |
activation,for |
R4972 |
T11502 |
T11492 |
prep |
through,circumvent |
R4973 |
T11503 |
T11504 |
amod |
specific,cofactors |
R4974 |
T11504 |
T11502 |
pobj |
cofactors,through |
R4975 |
T11505 |
T11506 |
mark |
while,maintaining |
R4976 |
T11506 |
T11492 |
advcl |
maintaining,circumvent |
R4977 |
T11507 |
T11506 |
advmod |
still,maintaining |
R4978 |
T11508 |
T11509 |
compound |
ETS,dependence |
R4979 |
T11509 |
T11506 |
dobj |
dependence,maintaining |
R4980 |
T11510 |
T11509 |
compound |
site,dependence |
R4981 |
T11511 |
T11492 |
punct |
", ",circumvent |
R4982 |
T11512 |
T11513 |
advmod |
thus,rendering |
R4983 |
T11513 |
T11492 |
advcl |
rendering,circumvent |
R4984 |
T11514 |
T11515 |
compound |
EWS,Pea3 |
R4985 |
T11515 |
T11513 |
dobj |
Pea3,rendering |
R4986 |
T11516 |
T11515 |
punct |
-,Pea3 |
R4987 |
T11517 |
T11518 |
advmod |
less,sensitive |
R4988 |
T11518 |
T11513 |
oprd |
sensitive,rendering |
R4989 |
T11519 |
T11518 |
prep |
to,sensitive |
R4990 |
T11520 |
T11521 |
det |
the,context |
R4991 |
T11521 |
T11519 |
pobj |
context,to |
R4992 |
T11522 |
T11521 |
amod |
cellular,context |
R4993 |
T11523 |
T11518 |
prep |
than,sensitive |
R4994 |
T11524 |
T11525 |
amod |
endogenous,factors |
R4995 |
T11525 |
T11523 |
pobj |
factors,than |
R4996 |
T11526 |
T11525 |
compound |
ETS,factors |
R4997 |
T11527 |
T11525 |
compound |
transcription,factors |
R4998 |
T11528 |
T11492 |
punct |
.,circumvent |
R4999 |
T11530 |
T11531 |
advcl |
Using,demonstrate |
R5000 |
T11532 |
T11533 |
det |
this,protein |
R5001 |
T11533 |
T11530 |
dobj |
protein,Using |
R5002 |
T11534 |
T11533 |
compound |
fusion,protein |
R5003 |
T11535 |
T11531 |
punct |
", ",demonstrate |
R5004 |
T11536 |
T11537 |
poss |
our,experiments |
R5005 |
T11537 |
T11531 |
nsubj |
experiments,demonstrate |
R5006 |
T11538 |
T11539 |
det |
a,change |
R5007 |
T11539 |
T11531 |
dobj |
change,demonstrate |
R5008 |
T11540 |
T11539 |
amod |
profound,change |
R5009 |
T11541 |
T11539 |
prep |
in,change |
R5010 |
T11542 |
T11543 |
det |
the,action |
R5011 |
T11543 |
T11541 |
pobj |
action,in |
R5012 |
T11544 |
T11543 |
prep |
of,action |
R5013 |
T11545 |
T11546 |
compound |
ETS,signaling |
R5014 |
T11546 |
T11544 |
pobj |
signaling,of |
R5015 |
T11547 |
T11531 |
prep |
at,demonstrate |
R5016 |
T11548 |
T11549 |
det |
the,level |
R5017 |
T11549 |
T11547 |
pobj |
level,at |
R5018 |
T11550 |
T11549 |
prep |
of,level |
R5019 |
T11551 |
T11552 |
amod |
transcriptional,regulation |
R5020 |
T11552 |
T11550 |
pobj |
regulation,of |
R5021 |
T11553 |
T11549 |
prep |
within,level |
R5022 |
T11554 |
T11555 |
amod |
post-mitotic,neurons |
R5023 |
T11555 |
T11553 |
pobj |
neurons,within |
R5024 |
T11556 |
T11555 |
compound |
DRG,neurons |
R5025 |
T11557 |
T11531 |
prep |
over,demonstrate |
R5026 |
T11558 |
T11557 |
pobj |
time,over |
R5027 |
T11559 |
T11531 |
punct |
.,demonstrate |
R5028 |
T11561 |
T11562 |
advmod |
Moreover,paired |
R5029 |
T11563 |
T11562 |
punct |
", ",paired |
R5030 |
T11564 |
T11565 |
det |
the,shift |
R5031 |
T11565 |
T11562 |
nsubjpass |
shift,paired |
R5032 |
T11566 |
T11565 |
amod |
observed,shift |
R5033 |
T11567 |
T11565 |
amod |
transcriptional,shift |
R5034 |
T11568 |
T11565 |
prep |
in,shift |
R5035 |
T11569 |
T11570 |
compound |
ETS,signaling |
R5036 |
T11570 |
T11568 |
pobj |
signaling,in |
R5037 |
T11571 |
T11562 |
auxpass |
is,paired |
R5038 |
T11572 |
T11562 |
prep |
with,paired |
R5039 |
T11573 |
T11574 |
det |
the,onset |
R5040 |
T11574 |
T11572 |
pobj |
onset,with |
R5041 |
T11575 |
T11574 |
prep |
of,onset |
R5042 |
T11576 |
T11577 |
amod |
appropriate,regulation |
R5043 |
T11577 |
T11575 |
pobj |
regulation,of |
R5044 |
T11578 |
T11577 |
prep |
of,regulation |
R5045 |
T11579 |
T11580 |
amod |
neuronal,specification |
R5046 |
T11580 |
T11578 |
pobj |
specification,of |
R5047 |
T11581 |
T11580 |
compound |
subtype,specification |
R5048 |
T11582 |
T11577 |
cc |
and,regulation |
R5049 |
T11583 |
T11577 |
conj |
establishment,regulation |
R5050 |
T11584 |
T11583 |
prep |
of,establishment |
R5051 |
T11585 |
T11586 |
amod |
axonal,projections |
R5052 |
T11586 |
T11584 |
pobj |
projections,of |
R5053 |
T11587 |
T11586 |
prep |
into,projections |
R5054 |
T11588 |
T11589 |
det |
the,area |
R5055 |
T11589 |
T11587 |
pobj |
area,into |
R5056 |
T11590 |
T11589 |
compound |
target,area |
R5057 |
T11591 |
T11562 |
punct |
.,paired |
R5058 |
T11593 |
T11594 |
amod |
Recent,experiments |
R5059 |
T11594 |
T11595 |
nsubj |
experiments,adds |
R5060 |
T11596 |
T11594 |
acl |
addressing,experiments |
R5061 |
T11597 |
T11598 |
det |
the,constraints |
R5062 |
T11598 |
T11596 |
dobj |
constraints,addressing |
R5063 |
T11599 |
T11598 |
amod |
temporal,constraints |
R5064 |
T11600 |
T11598 |
prep |
of,constraints |
R5065 |
T11601 |
T11602 |
compound |
transcription,factor |
R5066 |
T11602 |
T11603 |
compound |
factor,action |
R5067 |
T11603 |
T11600 |
pobj |
action,of |
R5068 |
T11604 |
T11596 |
prep |
in,addressing |
R5069 |
T11605 |
T11606 |
amod |
proliferating,cells |
R5070 |
T11606 |
T11604 |
pobj |
cells,in |
R5071 |
T11607 |
T11608 |
amod |
neural,progenitor |
R5072 |
T11608 |
T11606 |
compound |
progenitor,cells |
R5073 |
T11609 |
T11595 |
prep |
to,adds |
R5074 |
T11610 |
T11611 |
det |
the,idea |
R5075 |
T11611 |
T11609 |
pobj |
idea,to |
R5076 |
T11612 |
T11613 |
mark |
that,are |
R5077 |
T11613 |
T11611 |
acl |
are,idea |
R5078 |
T11614 |
T11615 |
amod |
defined,windows |
R5079 |
T11615 |
T11613 |
nsubj |
windows,are |
R5080 |
T11616 |
T11615 |
amod |
temporal,windows |
R5081 |
T11617 |
T11615 |
punct |
", ",windows |
R5082 |
T11618 |
T11619 |
prep |
during,act |
R5083 |
T11619 |
T11615 |
relcl |
act,windows |
R5084 |
T11620 |
T11618 |
pobj |
which,during |
R5085 |
T11621 |
T11622 |
compound |
transcription,factors |
R5086 |
T11622 |
T11619 |
nsubj |
factors,act |
R5087 |
T11623 |
T11624 |
aux |
to,control |
R5088 |
T11624 |
T11619 |
advcl |
control,act |
R5089 |
T11625 |
T11626 |
amod |
distinct,genes |
R5090 |
T11626 |
T11624 |
dobj |
genes,control |
R5091 |
T11627 |
T11626 |
amod |
downstream,genes |
R5092 |
T11628 |
T11626 |
compound |
target,genes |
R5093 |
T11629 |
T11613 |
punct |
", ",are |
R5094 |
T11630 |
T11613 |
prep |
of,are |
R5095 |
T11631 |
T11632 |
amod |
key,importance |
R5096 |
T11632 |
T11630 |
pobj |
importance,of |
R5097 |
T11633 |
T11632 |
prep |
to,importance |
R5098 |
T11634 |
T11635 |
amod |
neuronal,acquisition |
R5099 |
T11635 |
T11633 |
pobj |
acquisition,to |
R5100 |
T11636 |
T11635 |
compound |
fate,acquisition |
R5101 |
T11637 |
T11595 |
punct |
.,adds |
R5102 |
T11639 |
T11640 |
prep |
During,controls |
R5103 |
T11641 |
T11642 |
compound |
Drosophila,generation |
R5104 |
T11642 |
T11639 |
pobj |
generation,During |
R5105 |
T11643 |
T11642 |
compound |
neuroblast,generation |
R5106 |
T11644 |
T11640 |
punct |
", ",controls |
R5107 |
T11645 |
T11646 |
det |
the,Hunchback |
R5108 |
T11646 |
T11640 |
nsubj |
Hunchback,controls |
R5109 |
T11647 |
T11648 |
compound |
transcription,factor |
R5110 |
T11648 |
T11646 |
compound |
factor,Hunchback |
R5111 |
T11649 |
T11640 |
dobj |
specification,controls |
R5112 |
T11650 |
T11649 |
cc |
and,specification |
R5113 |
T11651 |
T11649 |
conj |
differentiation,specification |
R5114 |
T11652 |
T11649 |
prep |
of,specification |
R5115 |
T11653 |
T11654 |
advmod |
early,born |
R5116 |
T11654 |
T11656 |
amod |
born,neuroblasts |
R5117 |
T11655 |
T11654 |
punct |
-,born |
R5118 |
T11656 |
T11652 |
pobj |
neuroblasts,of |
R5119 |
T11657 |
T11658 |
punct |
[,38 |
R5120 |
T11658 |
T11640 |
parataxis |
38,controls |
R5121 |
T11659 |
T11658 |
punct |
],38 |
R5122 |
T11660 |
T11640 |
punct |
.,controls |
R5123 |
T11662 |
T11663 |
prep |
Over,lose |
R5124 |
T11664 |
T11662 |
pobj |
time,Over |
R5125 |
T11665 |
T11663 |
punct |
", ",lose |
R5126 |
T11666 |
T11663 |
advmod |
however,lose |
R5127 |
T11667 |
T11663 |
punct |
", ",lose |
R5128 |
T11668 |
T11663 |
nsubj |
neuroblasts,lose |
R5129 |
T11669 |
T11663 |
advmod |
progressively,lose |
R5130 |
T11670 |
T11671 |
poss |
their,competence |
R5131 |
T11671 |
T11663 |
dobj |
competence,lose |
R5132 |
T11672 |
T11673 |
aux |
to,generate |
R5133 |
T11673 |
T11671 |
acl |
generate,competence |
R5134 |
T11674 |
T11673 |
dobj |
cells,generate |
R5135 |
T11675 |
T11674 |
prep |
of,cells |
R5136 |
T11676 |
T11677 |
det |
an,fate |
R5137 |
T11677 |
T11675 |
pobj |
fate,of |
R5138 |
T11678 |
T11677 |
amod |
early,fate |
R5139 |
T11679 |
T11673 |
prep |
in,generate |
R5140 |
T11680 |
T11679 |
pobj |
response,in |
R5141 |
T11681 |
T11680 |
prep |
to,response |
R5142 |
T11682 |
T11683 |
compound |
Hunchback,expression |
R5143 |
T11683 |
T11681 |
pobj |
expression,to |
R5144 |
T11684 |
T11685 |
punct |
[,39 |
R5145 |
T11685 |
T11663 |
parataxis |
39,lose |
R5146 |
T11686 |
T11685 |
punct |
],39 |
R5147 |
T11687 |
T11663 |
punct |
.,lose |
R5148 |
T11689 |
T11690 |
det |
These,findings |
R5149 |
T11690 |
T11691 |
nsubj |
findings,argue |
R5150 |
T11692 |
T11691 |
advmod |
thus,argue |
R5151 |
T11693 |
T11691 |
advmod |
also,argue |
R5152 |
T11694 |
T11691 |
prep |
for,argue |
R5153 |
T11695 |
T11696 |
det |
a,change |
R5154 |
T11696 |
T11694 |
pobj |
change,for |
R5155 |
T11697 |
T11696 |
prep |
in,change |
R5156 |
T11698 |
T11699 |
amod |
cellular,competence |
R5157 |
T11699 |
T11697 |
pobj |
competence,in |
R5158 |
T11700 |
T11701 |
aux |
to,respond |
R5159 |
T11701 |
T11699 |
acl |
respond,competence |
R5160 |
T11702 |
T11701 |
prep |
to,respond |
R5161 |
T11703 |
T11704 |
det |
a,factor |
R5162 |
T11704 |
T11702 |
pobj |
factor,to |
R5163 |
T11705 |
T11704 |
amod |
specific,factor |
R5164 |
T11706 |
T11704 |
compound |
transcription,factor |
R5165 |
T11707 |
T11701 |
prep |
over,respond |
R5166 |
T11708 |
T11707 |
pobj |
time,over |
R5167 |
T11709 |
T11710 |
dep |
albeit,in |
R5168 |
T11710 |
T11701 |
prep |
in,respond |
R5169 |
T11711 |
T11712 |
det |
an,context |
R5170 |
T11712 |
T11710 |
pobj |
context,in |
R5171 |
T11713 |
T11712 |
amod |
early,context |
R5172 |
T11714 |
T11712 |
compound |
precursor,context |
R5173 |
T11715 |
T11691 |
punct |
.,argue |
R5174 |
T11717 |
T11718 |
advmod |
More,generally |
R5175 |
T11718 |
T11719 |
advmod |
generally,shown |
R5176 |
T11720 |
T11719 |
punct |
", ",shown |
R5177 |
T11721 |
T11719 |
prep |
during,shown |
R5179 |
T11722 |
T11723 |
det |
the,differentiation |
R5181 |
T11723 |
T11721 |
pobj |
differentiation,during |
R5183 |
T11724 |
T11723 |
prep |
of,differentiation |
R5184 |
T11735 |
T11719 |
xcomp |
exhibit,shown |
R5185 |
T11736 |
T11737 |
amod |
distinct,functions |
R5186 |
T11737 |
T11735 |
dobj |
functions,exhibit |
R5187 |
T11725 |
T11726 |
amod |
hematopoietic,lineages |
R5188 |
T11738 |
T11735 |
prep |
at,exhibit |
R5189 |
T11739 |
T11740 |
amod |
progressive,steps |
R5190 |
T11740 |
T11738 |
pobj |
steps,at |
R5191 |
T11726 |
T11724 |
pobj |
lineages,of |
R5192 |
T11741 |
T11740 |
prep |
of,steps |
R5193 |
T11742 |
T11743 |
compound |
lineage,specification |
R5194 |
T11743 |
T11741 |
pobj |
specification,of |
R5195 |
T11727 |
T11719 |
punct |
", ",shown |
R5196 |
T11744 |
T11745 |
punct |
[,40 |
R5197 |
T11745 |
T11735 |
parataxis |
40,exhibit |
R5198 |
T11728 |
T11729 |
amod |
several,factors |
R5199 |
T11746 |
T11745 |
punct |
],40 |
R5200 |
T11747 |
T11719 |
punct |
.,shown |
R5201 |
T11749 |
T11750 |
nsubj |
Analysis,provide |
R5202 |
T11729 |
T11719 |
nsubjpass |
factors,shown |
R5203 |
T11751 |
T11749 |
prep |
of,Analysis |
R5204 |
T11752 |
T11753 |
det |
the,mechanisms |
R5205 |
T11753 |
T11751 |
pobj |
mechanisms,of |
R5206 |
T11754 |
T11755 |
prep |
by,shifted |
R5207 |
T11730 |
T11729 |
compound |
transcription,factors |
R5208 |
T11755 |
T11753 |
relcl |
shifted,mechanisms |
R5209 |
T11756 |
T11754 |
pobj |
which,by |
R5210 |
T11731 |
T11719 |
aux |
have,shown |
R5211 |
T11757 |
T11758 |
compound |
transcription,factor |
R5212 |
T11758 |
T11759 |
compound |
factor,programs |
R5213 |
T11759 |
T11755 |
nsubjpass |
programs,shifted |
R5214 |
T11760 |
T11755 |
aux |
can,shifted |
R5215 |
T11761 |
T11755 |
auxpass |
be,shifted |
R5216 |
T11762 |
T11755 |
prep |
over,shifted |
R5217 |
T11763 |
T11762 |
pobj |
time,over |
R5218 |
T11764 |
T11765 |
aux |
to,control |
R5219 |
T11765 |
T11755 |
advcl |
control,shifted |
R5220 |
T11766 |
T11767 |
amod |
different,complements |
R5221 |
T11767 |
T11765 |
dobj |
complements,control |
R5222 |
T11768 |
T11767 |
prep |
of,complements |
R5223 |
T11769 |
T11770 |
amod |
downstream,genes |
R5224 |
T11770 |
T11768 |
pobj |
genes,of |
R5225 |
T11771 |
T11767 |
cc |
and,complements |
R5226 |
T11772 |
T11771 |
advmod |
thus,and |
R5227 |
T11773 |
T11774 |
amod |
different,aspects |
R5228 |
T11774 |
T11767 |
conj |
aspects,complements |
R5229 |
T11775 |
T11774 |
prep |
of,aspects |
R5230 |
T11776 |
T11777 |
amod |
neuronal,fates |
R5231 |
T11777 |
T11775 |
pobj |
fates,of |
R5232 |
T11778 |
T11776 |
cc |
and,neuronal |
R5233 |
T11779 |
T11776 |
conj |
cellular,neuronal |
R5234 |
T11780 |
T11774 |
prep |
in,aspects |
R5235 |
T11781 |
T11782 |
compound |
progenitor,cells |
R5236 |
T11782 |
T11780 |
pobj |
cells,in |
R5237 |
T11783 |
T11782 |
cc |
or,cells |
R5238 |
T11784 |
T11785 |
amod |
post-mitotic,neurons |
R5239 |
T11785 |
T11782 |
conj |
neurons,cells |
R5240 |
T11786 |
T11750 |
aux |
may,provide |
R5241 |
T11787 |
T11788 |
amod |
further,insight |
R5242 |
T11788 |
T11750 |
dobj |
insight,provide |
R5243 |
T11789 |
T11788 |
prep |
into,insight |
R5244 |
T11790 |
T11791 |
det |
the,way |
R5245 |
T11791 |
T11789 |
pobj |
way,into |
R5246 |
T11792 |
T11793 |
prep |
in,act |
R5247 |
T11793 |
T11791 |
relcl |
act,way |
R5248 |
T11794 |
T11792 |
pobj |
which,in |
R5249 |
T11795 |
T11796 |
compound |
transcription,factors |
R5250 |
T11796 |
T11793 |
nsubj |
factors,act |
R5251 |
T11797 |
T11798 |
aux |
to,control |
R5252 |
T11798 |
T11793 |
advcl |
control,act |
R5253 |
T11799 |
T11800 |
det |
the,assembly |
R5254 |
T11800 |
T11798 |
dobj |
assembly,control |
R5255 |
T11801 |
T11800 |
prep |
of,assembly |
R5256 |
T11802 |
T11803 |
amod |
neuronal,circuits |
R5257 |
T11803 |
T11801 |
pobj |
circuits,of |
R5258 |
T11804 |
T11750 |
punct |
.,provide |
R5259 |
T12346 |
T12345 |
prep |
of,Generation |
R5260 |
T12347 |
T12348 |
amod |
transgenic,mice |
R5261 |
T12348 |
T12346 |
pobj |
mice,of |
R5262 |
T12349 |
T12345 |
cc |
and,Generation |
R5263 |
T12350 |
T12351 |
compound |
mouse,genetics |
R5264 |
T12351 |
T12345 |
conj |
genetics,Generation |
R5265 |
T12353 |
T12354 |
compound |
Er81EWS,Pea3 |
R5266 |
T12354 |
T12356 |
compound |
Pea3,mice |
R5267 |
T12355 |
T12354 |
punct |
-,Pea3 |
R5268 |
T12356 |
T12357 |
nsubjpass |
mice,generated |
R5269 |
T12358 |
T12357 |
auxpass |
were,generated |
R5270 |
T12359 |
T12357 |
advcl |
following,generated |
R5271 |
T12360 |
T12361 |
det |
a,strategy |
R5272 |
T12361 |
T12359 |
dobj |
strategy,following |
R5273 |
T12362 |
T12361 |
amod |
similar,strategy |
R5274 |
T12363 |
T12362 |
prep |
to,similar |
R5275 |
T12364 |
T12363 |
pobj |
that,to |
R5276 |
T12365 |
T12364 |
acl |
described,that |
R5277 |
T12366 |
T12365 |
prep |
for,described |
R5278 |
T12367 |
T12368 |
det |
the,generation |
R5279 |
T12368 |
T12366 |
pobj |
generation,for |
R5280 |
T12369 |
T12368 |
prep |
of,generation |
R5281 |
T12370 |
T12371 |
compound |
Er81NLZ,mice |
R5282 |
T12371 |
T12369 |
pobj |
mice,of |
R5283 |
T12372 |
T12373 |
punct |
[,14 |
R5284 |
T12373 |
T12365 |
parataxis |
14,described |
R5285 |
T12374 |
T12373 |
punct |
],14 |
R5286 |
T12375 |
T12357 |
punct |
.,generated |
R5287 |
T12377 |
T12378 |
prep |
In,inserted |
R5288 |
T12379 |
T12377 |
pobj |
brief,In |
R5289 |
T12380 |
T12378 |
punct |
", ",inserted |
R5290 |
T12381 |
T12382 |
det |
a,vector |
R5291 |
T12382 |
T12378 |
nsubjpass |
vector,inserted |
R5292 |
T12383 |
T12382 |
compound |
targeting,vector |
R5293 |
T12384 |
T12382 |
prep |
with,vector |
R5294 |
T12385 |
T12386 |
det |
a,coding |
R5295 |
T12386 |
T12384 |
pobj |
coding,with |
R5296 |
T12387 |
T12386 |
compound |
cDNA,coding |
R5297 |
T12388 |
T12382 |
prep |
for,vector |
R5298 |
T12389 |
T12390 |
compound |
EWS,Pea3 |
R5299 |
T12390 |
T12388 |
pobj |
Pea3,for |
R5300 |
T12391 |
T12390 |
punct |
-,Pea3 |
R5301 |
T12392 |
T12378 |
auxpass |
was,inserted |
R5302 |
T12393 |
T12378 |
prep |
in,inserted |
R5303 |
T12394 |
T12393 |
pobj |
frame,in |
R5304 |
T12395 |
T12394 |
prep |
with,frame |
R5305 |
T12396 |
T12397 |
det |
the,ATG |
R5306 |
T12397 |
T12395 |
pobj |
ATG,with |
R5307 |
T12398 |
T12397 |
amod |
endogenous,ATG |
R5308 |
T12399 |
T12397 |
compound |
start,ATG |
R5309 |
T12400 |
T12378 |
prep |
into,inserted |
R5310 |
T12401 |
T12400 |
pobj |
exon,into |
R5311 |
T12402 |
T12401 |
nummod |
2,exon |
R5312 |
T12403 |
T12401 |
prep |
of,exon |
R5313 |
T12404 |
T12405 |
det |
the,locus |
R5314 |
T12405 |
T12403 |
pobj |
locus,of |
R5315 |
T12406 |
T12405 |
nmod |
Er81,locus |
R5316 |
T12407 |
T12405 |
amod |
genomic,locus |
R5317 |
T12408 |
T12378 |
cc |
and,inserted |
R5318 |
T12409 |
T12378 |
conj |
used,inserted |
R5319 |
T12410 |
T12409 |
prep |
for,used |
R5320 |
T12411 |
T12412 |
amod |
homologous,recombination |
R5321 |
T12412 |
T12410 |
pobj |
recombination,for |
R5322 |
T12413 |
T12409 |
prep |
in,used |
R5323 |
T12414 |
T12415 |
compound |
W95,cells |
R5324 |
T12415 |
T12413 |
pobj |
cells,in |
R5325 |
T12416 |
T12415 |
compound |
ES,cells |
R5326 |
T12417 |
T12378 |
punct |
.,inserted |
R5327 |
T12419 |
T12420 |
compound |
EWS,Pea3 |
R5328 |
T12420 |
T12422 |
nsubj |
Pea3,represents |
R5329 |
T12421 |
T12420 |
punct |
-,Pea3 |
R5330 |
T12423 |
T12424 |
det |
a,gene |
R5331 |
T12424 |
T12422 |
dobj |
gene,represents |
R5332 |
T12425 |
T12424 |
compound |
fusion,gene |
R5333 |
T12426 |
T12424 |
prep |
between,gene |
R5334 |
T12427 |
T12428 |
det |
the,terminal |
R5335 |
T12428 |
T12426 |
pobj |
terminal,between |
R5336 |
T12429 |
T12428 |
compound |
amino,terminal |
R5337 |
T12430 |
T12428 |
prep |
of,terminal |
R5338 |
T12431 |
T12430 |
pobj |
EWS,of |
R5339 |
T12432 |
T12428 |
cc |
and,terminal |
R5340 |
T12433 |
T12434 |
det |
the,domain |
R5341 |
T12434 |
T12428 |
conj |
domain,terminal |
R5342 |
T12435 |
T12434 |
compound |
ETS,domain |
R5343 |
T12436 |
T12434 |
prep |
of,domain |
R5344 |
T12437 |
T12436 |
pobj |
Pea3,of |
R5345 |
T12438 |
T12439 |
punct |
[,20 |
R5346 |
T12439 |
T12422 |
parataxis |
20,represents |
R5347 |
T12440 |
T12439 |
punct |
],20 |
R5348 |
T12441 |
T12422 |
punct |
.,represents |
R5349 |
T12443 |
T12444 |
det |
The,pair |
R5350 |
T12444 |
T12446 |
nsubj |
pair,was |
R5351 |
T12445 |
T12444 |
compound |
primer,pair |
R5352 |
T12447 |
T12444 |
acl |
used,pair |
R5353 |
T12448 |
T12449 |
aux |
to,detect |
R5354 |
T12449 |
T12447 |
advcl |
detect,used |
R5355 |
T12450 |
T12449 |
advmod |
specifically,detect |
R5356 |
T12451 |
T12452 |
det |
the,allele |
R5357 |
T12452 |
T12449 |
dobj |
allele,detect |
R5358 |
T12453 |
T12454 |
compound |
Er81EWS,Pea3 |
R5359 |
T12454 |
T12452 |
compound |
Pea3,allele |
R5360 |
T12455 |
T12454 |
punct |
-,Pea3 |
R5361 |
T12456 |
T12457 |
nummod |
5,CAGCCACTGCACCTACAAGAC |
R5362 |
T12457 |
T12446 |
attr |
CAGCCACTGCACCTACAAGAC,was |
R5363 |
T12458 |
T12456 |
punct |
′,5 |
R5364 |
T12459 |
T12457 |
punct |
-,CAGCCACTGCACCTACAAGAC |
R5365 |
T12460 |
T12457 |
punct |
-,CAGCCACTGCACCTACAAGAC |
R5366 |
T12461 |
T12457 |
nummod |
3,CAGCCACTGCACCTACAAGAC |
R5367 |
T12462 |
T12457 |
punct |
′,CAGCCACTGCACCTACAAGAC |
R5368 |
T12463 |
T12457 |
cc |
and,CAGCCACTGCACCTACAAGAC |
R5369 |
T12464 |
T12465 |
nummod |
5,CTTCCTGCTTGATGTCTCCTTC |
R5370 |
T12465 |
T12457 |
conj |
CTTCCTGCTTGATGTCTCCTTC,CAGCCACTGCACCTACAAGAC |
R5371 |
T12466 |
T12464 |
punct |
′,5 |
R5372 |
T12467 |
T12465 |
punct |
-,CTTCCTGCTTGATGTCTCCTTC |
R5373 |
T12468 |
T12465 |
punct |
-,CTTCCTGCTTGATGTCTCCTTC |
R5374 |
T12469 |
T12465 |
nummod |
3,CTTCCTGCTTGATGTCTCCTTC |
R5375 |
T12470 |
T12465 |
punct |
′,CTTCCTGCTTGATGTCTCCTTC |
R5376 |
T12471 |
T12446 |
punct |
.,was |
R5377 |
T12473 |
T12474 |
prep |
For,integrated |
R5378 |
T12475 |
T12476 |
det |
the,generation |
R5379 |
T12476 |
T12473 |
pobj |
generation,For |
R5380 |
T12477 |
T12476 |
prep |
of,generation |
R5381 |
T12478 |
T12479 |
nmod |
TaumGFP,mice |
R5382 |
T12479 |
T12477 |
pobj |
mice,of |
R5383 |
T12480 |
T12478 |
cc |
and,TaumGFP |
R5384 |
T12481 |
T12482 |
compound |
TauEWS,Pea3 |
R5385 |
T12482 |
T12478 |
conj |
Pea3,TaumGFP |
R5386 |
T12483 |
T12482 |
punct |
-,Pea3 |
R5387 |
T12484 |
T12474 |
punct |
", ",integrated |
R5388 |
T12485 |
T12486 |
nmod |
lox,pA |
R5389 |
T12486 |
T12500 |
nmod |
pA,cassettes |
R5390 |
T12487 |
T12486 |
punct |
-,pA |
R5391 |
T12488 |
T12486 |
nmod |
STOP,pA |
R5392 |
T12489 |
T12486 |
punct |
-,pA |
R5393 |
T12490 |
T12486 |
nmod |
lox,pA |
R5394 |
T12491 |
T12486 |
punct |
-,pA |
R5395 |
T12492 |
T12486 |
nmod |
mGFP,pA |
R5396 |
T12493 |
T12486 |
punct |
-,pA |
R5397 |
T12494 |
T12486 |
nmod |
IRES,pA |
R5398 |
T12495 |
T12486 |
punct |
-,pA |
R5399 |
T12496 |
T12486 |
nmod |
NLS,pA |
R5400 |
T12497 |
T12486 |
punct |
-,pA |
R5401 |
T12498 |
T12486 |
nmod |
LacZ,pA |
R5402 |
T12499 |
T12486 |
punct |
-,pA |
R5403 |
T12500 |
T12474 |
nsubjpass |
cassettes,integrated |
R5404 |
T12501 |
T12486 |
cc |
and,pA |
R5405 |
T12502 |
T12503 |
compound |
lox,pA |
R5406 |
T12503 |
T12486 |
conj |
pA,pA |
R5407 |
T12504 |
T12503 |
punct |
-,pA |
R5408 |
T12505 |
T12503 |
compound |
STOP,pA |
R5409 |
T12506 |
T12503 |
punct |
-,pA |
R5410 |
T12507 |
T12503 |
compound |
lox,pA |
R5411 |
T12508 |
T12503 |
punct |
-,pA |
R5412 |
T12509 |
T12503 |
compound |
EWS,pA |
R5413 |
T12510 |
T12503 |
punct |
-,pA |
R5414 |
T12511 |
T12503 |
compound |
Pea3,pA |
R5415 |
T12512 |
T12503 |
punct |
-,pA |
R5416 |
T12513 |
T12503 |
compound |
IRES,pA |
R5417 |
T12514 |
T12503 |
punct |
-,pA |
R5418 |
T12515 |
T12503 |
compound |
NLS,pA |
R5419 |
T12516 |
T12503 |
punct |
-,pA |
R5420 |
T12517 |
T12503 |
compound |
LacZ,pA |
R5421 |
T12518 |
T12503 |
punct |
-,pA |
R5422 |
T12519 |
T12500 |
compound |
targeting,cassettes |
R5423 |
T12520 |
T12474 |
auxpass |
were,integrated |
R5424 |
T12521 |
T12474 |
prep |
into,integrated |
R5425 |
T12522 |
T12521 |
pobj |
exon,into |
R5426 |
T12523 |
T12522 |
nummod |
2,exon |
R5427 |
T12524 |
T12522 |
prep |
of,exon |
R5428 |
T12525 |
T12526 |
det |
the,locus |
R5429 |
T12526 |
T12524 |
pobj |
locus,of |
R5430 |
T12527 |
T12526 |
nmod |
Tau,locus |
R5431 |
T12528 |
T12526 |
amod |
genomic,locus |
R5432 |
T12529 |
T12530 |
punct |
(,removed |
R5433 |
T12530 |
T12474 |
parataxis |
removed,integrated |
R5434 |
T12531 |
T12532 |
det |
the,ATG |
R5435 |
T12532 |
T12530 |
nsubjpass |
ATG,removed |
R5436 |
T12533 |
T12532 |
amod |
endogenous,ATG |
R5437 |
T12534 |
T12532 |
compound |
start,ATG |
R5438 |
T12535 |
T12530 |
auxpass |
was,removed |
R5439 |
T12536 |
T12530 |
prep |
in,removed |
R5440 |
T12537 |
T12538 |
det |
the,vectors |
R5441 |
T12538 |
T12536 |
pobj |
vectors,in |
R5442 |
T12539 |
T12538 |
compound |
targeting,vectors |
R5443 |
T12540 |
T12530 |
punct |
;,removed |
R5444 |
T12541 |
T12542 |
nsubj |
details,available |
R5445 |
T12542 |
T12530 |
amod |
available,removed |
R5446 |
T12543 |
T12542 |
prep |
upon,available |
R5447 |
T12544 |
T12543 |
pobj |
request,upon |
R5448 |
T12545 |
T12530 |
punct |
),removed |
R5449 |
T12546 |
T12474 |
punct |
.,integrated |
R5450 |
T12548 |
T12549 |
nsubjpass |
mGFP,provided |
R5451 |
T12550 |
T12549 |
auxpass |
was,provided |
R5452 |
T12551 |
T12549 |
agent |
by,provided |
R5453 |
T12552 |
T12553 |
compound |
P.,Caroni |
R5454 |
T12553 |
T12551 |
pobj |
Caroni,by |
R5455 |
T12554 |
T12555 |
punct |
[,25 |
R5456 |
T12555 |
T12549 |
parataxis |
25,provided |
R5457 |
T12556 |
T12555 |
punct |
],25 |
R5458 |
T12557 |
T12549 |
punct |
.,provided |
R5459 |
T12559 |
T12560 |
compound |
ES,recombinants |
R5460 |
T12560 |
T12562 |
nsubjpass |
recombinants,screened |
R5461 |
T12561 |
T12560 |
compound |
cell,recombinants |
R5462 |
T12563 |
T12562 |
auxpass |
were,screened |
R5463 |
T12564 |
T12562 |
prep |
by,screened |
R5464 |
T12565 |
T12566 |
compound |
Southern,blot |
R5465 |
T12566 |
T12567 |
compound |
blot,analysis |
R5466 |
T12567 |
T12564 |
pobj |
analysis,by |
R5467 |
T12568 |
T12562 |
advcl |
using,screened |
R5468 |
T12569 |
T12570 |
det |
the,probe |
R5469 |
T12570 |
T12568 |
dobj |
probe,using |
R5470 |
T12571 |
T12568 |
prep |
in,using |
R5471 |
T12572 |
T12573 |
det |
the,region |
R5472 |
T12573 |
T12571 |
pobj |
region,in |
R5473 |
T12574 |
T12573 |
nummod |
5,region |
R5474 |
T12575 |
T12574 |
punct |
′,5 |
R5475 |
T12576 |
T12577 |
mark |
as,described |
R5476 |
T12577 |
T12568 |
advcl |
described,using |
R5477 |
T12578 |
T12577 |
advmod |
previously,described |
R5478 |
T12579 |
T12580 |
punct |
[,41 |
R5479 |
T12580 |
T12577 |
parataxis |
41,described |
R5480 |
T12581 |
T12580 |
punct |
],41 |
R5481 |
T12582 |
T12562 |
punct |
.,screened |
R5482 |
T12584 |
T12585 |
nsubj |
Frequency,was |
R5483 |
T12586 |
T12584 |
prep |
of,Frequency |
R5484 |
T12587 |
T12586 |
pobj |
recombination,of |
R5485 |
T12588 |
T12587 |
prep |
in,recombination |
R5486 |
T12589 |
T12590 |
nummod |
129,Ola |
R5487 |
T12590 |
T12592 |
compound |
Ola,cells |
R5488 |
T12591 |
T12590 |
punct |
/,Ola |
R5489 |
T12592 |
T12588 |
pobj |
cells,in |
R5490 |
T12593 |
T12592 |
compound |
ES,cells |
R5491 |
T12594 |
T12595 |
advmod |
approximately,3 |
R5492 |
T12595 |
T12585 |
attr |
3,was |
R5493 |
T12596 |
T12595 |
quantmod |
1,3 |
R5494 |
T12597 |
T12595 |
punct |
/,3 |
R5495 |
T12598 |
T12585 |
prep |
for,was |
R5496 |
T12599 |
T12600 |
det |
both,constructs |
R5497 |
T12600 |
T12598 |
pobj |
constructs,for |
R5498 |
T12601 |
T12600 |
compound |
Tau,constructs |
R5499 |
T12602 |
T12585 |
punct |
.,was |
R5500 |
T12604 |
T12605 |
prep |
For,obtained |
R5501 |
T12606 |
T12607 |
det |
the,generation |
R5502 |
T12607 |
T12604 |
pobj |
generation,For |
R5503 |
T12608 |
T12607 |
prep |
of,generation |
R5504 |
T12609 |
T12610 |
compound |
PVCre,mice |
R5505 |
T12610 |
T12608 |
pobj |
mice,of |
R5506 |
T12611 |
T12605 |
punct |
", ",obtained |
R5507 |
T12612 |
T12613 |
nmod |
mouse,clones |
R5508 |
T12613 |
T12605 |
nsubjpass |
clones,obtained |
R5509 |
T12614 |
T12613 |
amod |
genomic,clones |
R5510 |
T12615 |
T12605 |
auxpass |
were,obtained |
R5511 |
T12616 |
T12605 |
prep |
by,obtained |
R5512 |
T12617 |
T12616 |
pcomp |
screening,by |
R5513 |
T12618 |
T12619 |
det |
a,library |
R5514 |
T12619 |
T12617 |
dobj |
library,screening |
R5515 |
T12620 |
T12621 |
nmod |
129SV,J |
R5516 |
T12621 |
T12619 |
nmod |
J,library |
R5517 |
T12622 |
T12621 |
punct |
/,J |
R5518 |
T12623 |
T12619 |
amod |
genomic,library |
R5519 |
T12624 |
T12625 |
punct |
(,Incyte |
R5520 |
T12625 |
T12617 |
parataxis |
Incyte,screening |
R5521 |
T12626 |
T12625 |
punct |
", ",Incyte |
R5522 |
T12627 |
T12625 |
npadvmod |
Wilmington,Incyte |
R5523 |
T12628 |
T12625 |
punct |
", ",Incyte |
R5524 |
T12629 |
T12625 |
npadvmod |
Delaware,Incyte |
R5525 |
T12630 |
T12625 |
punct |
", ",Incyte |
R5526 |
T12631 |
T12632 |
compound |
United,States |
R5527 |
T12632 |
T12625 |
npadvmod |
States,Incyte |
R5528 |
T12633 |
T12625 |
punct |
),Incyte |
R5529 |
T12634 |
T12605 |
punct |
.,obtained |
R5530 |
T12636 |
T12637 |
prep |
For,see |
R5531 |
T12638 |
T12636 |
pobj |
details,For |
R5532 |
T12639 |
T12638 |
prep |
on,details |
R5533 |
T12640 |
T12641 |
det |
the,structure |
R5534 |
T12641 |
T12639 |
pobj |
structure,on |
R5535 |
T12642 |
T12641 |
amod |
genomic,structure |
R5536 |
T12643 |
T12641 |
prep |
of,structure |
R5537 |
T12644 |
T12645 |
det |
the,locus |
R5538 |
T12645 |
T12643 |
pobj |
locus,of |
R5539 |
T12646 |
T12645 |
compound |
mouse,locus |
R5540 |
T12647 |
T12645 |
compound |
PV,locus |
R5541 |
T12648 |
T12637 |
punct |
[,see |
R5542 |
T12649 |
T12637 |
dobj |
42,see |
R5543 |
T12650 |
T12637 |
punct |
],see |
R5544 |
T12651 |
T12637 |
punct |
.,see |
R5545 |
T12653 |
T12654 |
det |
An,cassette |
R5546 |
T12654 |
T12661 |
nsubjpass |
cassette,integrated |
R5547 |
T12655 |
T12656 |
compound |
IRES,pA |
R5548 |
T12656 |
T12654 |
compound |
pA,cassette |
R5549 |
T12657 |
T12656 |
punct |
-,pA |
R5550 |
T12658 |
T12656 |
compound |
Cre,pA |
R5551 |
T12659 |
T12656 |
punct |
-,pA |
R5552 |
T12660 |
T12654 |
compound |
targeting,cassette |
R5553 |
T12662 |
T12663 |
punct |
[,33 |
R5554 |
T12663 |
T12654 |
parataxis |
33,cassette |
R5555 |
T12664 |
T12663 |
punct |
],33 |
R5556 |
T12665 |
T12661 |
auxpass |
was,integrated |
R5557 |
T12666 |
T12661 |
prep |
into,integrated |
R5558 |
T12667 |
T12668 |
det |
the,UTR |
R5559 |
T12668 |
T12666 |
pobj |
UTR,into |
R5560 |
T12669 |
T12668 |
nummod |
3,UTR |
R5561 |
T12670 |
T12669 |
punct |
′,3 |
R5562 |
T12671 |
T12668 |
prep |
of,UTR |
R5563 |
T12672 |
T12671 |
pobj |
exon,of |
R5564 |
T12673 |
T12672 |
nummod |
5,exon |
R5565 |
T12674 |
T12661 |
punct |
", ",integrated |
R5566 |
T12675 |
T12661 |
cc |
and,integrated |
R5567 |
T12676 |
T12677 |
compound |
ES,recombinants |
R5568 |
T12677 |
T12679 |
nsubjpass |
recombinants,screened |
R5569 |
T12678 |
T12677 |
compound |
cell,recombinants |
R5570 |
T12679 |
T12661 |
conj |
screened,integrated |
R5571 |
T12680 |
T12679 |
auxpass |
were,screened |
R5572 |
T12681 |
T12679 |
prep |
with,screened |
R5573 |
T12682 |
T12683 |
det |
a,probe |
R5574 |
T12683 |
T12681 |
pobj |
probe,with |
R5575 |
T12684 |
T12683 |
nummod |
5,probe |
R5576 |
T12685 |
T12684 |
punct |
′,5 |
R5577 |
T12686 |
T12687 |
punct |
(,digest |
R5578 |
T12687 |
T12679 |
parataxis |
digest,screened |
R5579 |
T12688 |
T12687 |
dep |
oligos,digest |
R5580 |
T12689 |
T12688 |
punct |
", ",oligos |
R5581 |
T12690 |
T12691 |
nummod |
5,GAGATGACCCAGCCAGGATGCCTC |
R5582 |
T12691 |
T12688 |
conj |
GAGATGACCCAGCCAGGATGCCTC,oligos |
R5583 |
T12692 |
T12690 |
punct |
′,5 |
R5584 |
T12693 |
T12691 |
punct |
-,GAGATGACCCAGCCAGGATGCCTC |
R5585 |
T12694 |
T12691 |
punct |
-,GAGATGACCCAGCCAGGATGCCTC |
R5586 |
T12695 |
T12691 |
nummod |
3,GAGATGACCCAGCCAGGATGCCTC |
R5587 |
T12696 |
T12691 |
punct |
′,GAGATGACCCAGCCAGGATGCCTC |
R5588 |
T12697 |
T12691 |
cc |
and,GAGATGACCCAGCCAGGATGCCTC |
R5589 |
T12698 |
T12699 |
nummod |
5,CTGACCACTCTCGCTCCGGTGTCC |
R5590 |
T12699 |
T12691 |
conj |
CTGACCACTCTCGCTCCGGTGTCC,GAGATGACCCAGCCAGGATGCCTC |
R5591 |
T12700 |
T12698 |
punct |
′,5 |
R5592 |
T12701 |
T12699 |
punct |
-,CTGACCACTCTCGCTCCGGTGTCC |
R5593 |
T12702 |
T12699 |
punct |
-,CTGACCACTCTCGCTCCGGTGTCC |
R5594 |
T12703 |
T12699 |
nummod |
3,CTGACCACTCTCGCTCCGGTGTCC |
R5595 |
T12704 |
T12699 |
punct |
′,CTGACCACTCTCGCTCCGGTGTCC |
R5596 |
T12705 |
T12687 |
punct |
;,digest |
R5597 |
T12706 |
T12707 |
amod |
genomic,DNA |
R5598 |
T12707 |
T12687 |
dep |
DNA,digest |
R5599 |
T12708 |
T12687 |
punct |
", ",digest |
R5600 |
T12709 |
T12687 |
compound |
HindIII,digest |
R5601 |
T12710 |
T12687 |
punct |
),digest |
R5602 |
T12711 |
T12679 |
punct |
.,screened |
R5603 |
T12713 |
T12714 |
det |
The,frequency |
R5604 |
T12714 |
T12715 |
nsubj |
frequency,was |
R5605 |
T12716 |
T12714 |
prep |
of,frequency |
R5606 |
T12717 |
T12716 |
pobj |
recombination,of |
R5607 |
T12718 |
T12717 |
prep |
in,recombination |
R5608 |
T12719 |
T12720 |
nummod |
129,Ola |
R5609 |
T12720 |
T12722 |
compound |
Ola,cells |
R5610 |
T12721 |
T12720 |
punct |
/,Ola |
R5611 |
T12722 |
T12718 |
pobj |
cells,in |
R5612 |
T12723 |
T12722 |
compound |
ES,cells |
R5613 |
T12724 |
T12725 |
advmod |
approximately,20 |
R5614 |
T12725 |
T12715 |
attr |
20,was |
R5615 |
T12726 |
T12725 |
quantmod |
1,20 |
R5616 |
T12727 |
T12725 |
punct |
/,20 |
R5617 |
T12728 |
T12715 |
punct |
.,was |
R5618 |
T12730 |
T12731 |
amod |
Recombinant,clones |
R5619 |
T12731 |
T12732 |
nsubjpass |
clones,aggregated |
R5620 |
T12733 |
T12732 |
auxpass |
were,aggregated |
R5621 |
T12734 |
T12732 |
prep |
with,aggregated |
R5622 |
T12735 |
T12736 |
compound |
morula,stage |
R5623 |
T12736 |
T12737 |
compound |
stage,embryos |
R5624 |
T12737 |
T12734 |
pobj |
embryos,with |
R5625 |
T12738 |
T12739 |
aux |
to,generate |
R5626 |
T12739 |
T12732 |
advcl |
generate,aggregated |
R5627 |
T12740 |
T12741 |
amod |
chimeric,mice |
R5628 |
T12741 |
T12739 |
dobj |
mice,generate |
R5629 |
T12742 |
T12741 |
compound |
founder,mice |
R5630 |
T12743 |
T12744 |
dep |
that,transmitted |
R5631 |
T12744 |
T12741 |
relcl |
transmitted,mice |
R5632 |
T12745 |
T12746 |
det |
the,alleles |
R5633 |
T12746 |
T12744 |
dobj |
alleles,transmitted |
R5634 |
T12747 |
T12746 |
compound |
mutant,alleles |
R5635 |
T12748 |
T12732 |
punct |
.,aggregated |
R5636 |
T12750 |
T12751 |
prep |
In,were |
R5637 |
T12752 |
T12753 |
det |
all,experiments |
R5638 |
T12753 |
T12750 |
pobj |
experiments,In |
R5639 |
T12754 |
T12753 |
acl |
performed,experiments |
R5640 |
T12755 |
T12754 |
prep |
in,performed |
R5641 |
T12756 |
T12757 |
det |
this,study |
R5642 |
T12757 |
T12755 |
pobj |
study,in |
R5643 |
T12758 |
T12751 |
punct |
", ",were |
R5644 |
T12759 |
T12751 |
nsubj |
animals,were |
R5645 |
T12760 |
T12751 |
prep |
of,were |
R5646 |
T12761 |
T12762 |
amod |
mixed,background |
R5647 |
T12762 |
T12760 |
pobj |
background,of |
R5648 |
T12763 |
T12762 |
amod |
genetic,background |
R5649 |
T12764 |
T12765 |
punct |
(,Ola |
R5650 |
T12765 |
T12751 |
parataxis |
Ola,were |
R5651 |
T12766 |
T12765 |
nummod |
129,Ola |
R5652 |
T12767 |
T12765 |
punct |
/,Ola |
R5653 |
T12768 |
T12765 |
cc |
and,Ola |
R5654 |
T12769 |
T12765 |
conj |
C57Bl6,Ola |
R5655 |
T12770 |
T12765 |
punct |
),Ola |
R5656 |
T12771 |
T12751 |
punct |
.,were |
R5657 |
T12773 |
T12774 |
nmod |
Thy1spGFP,mice |
R5658 |
T12774 |
T12776 |
nsubjpass |
mice,generated |
R5659 |
T12775 |
T12774 |
amod |
transgenic,mice |
R5660 |
T12777 |
T12776 |
auxpass |
were,generated |
R5661 |
T12778 |
T12776 |
prep |
in,generated |
R5662 |
T12779 |
T12778 |
pobj |
analogy,in |
R5663 |
T12780 |
T12779 |
prep |
to,analogy |
R5664 |
T12781 |
T12782 |
compound |
De,Paola |
R5665 |
T12782 |
T12780 |
pobj |
Paola,to |
R5666 |
T12783 |
T12784 |
advmod |
et,al. |
R5667 |
T12784 |
T12782 |
advmod |
al.,Paola |
R5668 |
T12785 |
T12786 |
punct |
[,25 |
R5669 |
T12786 |
T12776 |
parataxis |
25,generated |
R5670 |
T12787 |
T12786 |
punct |
],25 |
R5671 |
T12788 |
T12776 |
punct |
", ",generated |
R5672 |
T12789 |
T12776 |
cc |
and,generated |
R5673 |
T12790 |
T12791 |
prep |
for,selected |
R5674 |
T12791 |
T12776 |
conj |
selected,generated |
R5675 |
T12792 |
T12793 |
det |
these,experiments |
R5676 |
T12793 |
T12790 |
pobj |
experiments,for |
R5677 |
T12794 |
T12795 |
det |
a,strain |
R5678 |
T12795 |
T12791 |
nsubjpass |
strain,selected |
R5679 |
T12796 |
T12795 |
prep |
of,strain |
R5680 |
T12797 |
T12796 |
pobj |
mice,of |
R5681 |
T12798 |
T12797 |
prep |
with,mice |
R5682 |
T12799 |
T12800 |
amod |
early,expression |
R5683 |
T12800 |
T12798 |
pobj |
expression,with |
R5684 |
T12801 |
T12800 |
amod |
embryonic,expression |
R5685 |
T12802 |
T12791 |
auxpass |
was,selected |
R5686 |
T12803 |
T12791 |
punct |
.,selected |
R5687 |
T12805 |
T12806 |
nmod |
Isl1Cre,strains |
R5688 |
T12806 |
T12810 |
nsubjpass |
strains,described |
R5689 |
T12807 |
T12805 |
cc |
and,Isl1Cre |
R5690 |
T12808 |
T12805 |
conj |
Hb9Cre,Isl1Cre |
R5691 |
T12809 |
T12806 |
compound |
mouse,strains |
R5692 |
T12811 |
T12810 |
aux |
have,described |
R5693 |
T12812 |
T12810 |
auxpass |
been,described |
R5694 |
T12813 |
T12814 |
punct |
[,43 |
R5695 |
T12814 |
T12810 |
parataxis |
43,described |
R5696 |
T12815 |
T12814 |
nummod |
33,43 |
R5697 |
T12816 |
T12814 |
punct |
",",43 |
R5698 |
T12817 |
T12814 |
punct |
],43 |
R5699 |
T12818 |
T12810 |
cc |
and,described |
R5700 |
T12819 |
T12820 |
nmod |
Bax,animals |
R5701 |
T12820 |
T12824 |
nsubj |
animals,were |
R5702 |
T12821 |
T12819 |
punct |
+,Bax |
R5703 |
T12822 |
T12819 |
punct |
/,Bax |
R5704 |
T12823 |
T12819 |
punct |
−,Bax |
R5705 |
T12824 |
T12810 |
conj |
were,described |
R5706 |
T12825 |
T12824 |
prep |
from,were |
R5707 |
T12826 |
T12827 |
compound |
Jackson,Laboratory |
R5708 |
T12827 |
T12825 |
pobj |
Laboratory,from |
R5709 |
T12828 |
T12829 |
punct |
(,Harbor |
R5710 |
T12829 |
T12824 |
parataxis |
Harbor,were |
R5711 |
T12830 |
T12829 |
compound |
Bar,Harbor |
R5712 |
T12831 |
T12829 |
punct |
", ",Harbor |
R5713 |
T12832 |
T12829 |
npadvmod |
Maine,Harbor |
R5714 |
T12833 |
T12829 |
punct |
", ",Harbor |
R5715 |
T12834 |
T12835 |
compound |
United,States |
R5716 |
T12835 |
T12829 |
npadvmod |
States,Harbor |
R5717 |
T12836 |
T12829 |
punct |
),Harbor |
R5718 |
T12837 |
T12838 |
punct |
[,27 |
R5719 |
T12838 |
T12824 |
parataxis |
27,were |
R5720 |
T12839 |
T12838 |
punct |
],27 |
R5721 |
T12840 |
T12824 |
punct |
.,were |
R5722 |
T12842 |
T12843 |
amod |
Timed,pregnancies |
R5723 |
T12843 |
T12844 |
nsubjpass |
pregnancies,set |
R5724 |
T12845 |
T12844 |
auxpass |
were,set |
R5725 |
T12846 |
T12844 |
prt |
up,set |
R5726 |
T12847 |
T12848 |
aux |
to,generate |
R5727 |
T12848 |
T12844 |
advcl |
generate,set |
R5728 |
T12849 |
T12848 |
dobj |
embryos,generate |
R5729 |
T12850 |
T12849 |
prep |
of,embryos |
R5730 |
T12851 |
T12852 |
amod |
different,stages |
R5731 |
T12852 |
T12850 |
pobj |
stages,of |
R5732 |
T12853 |
T12852 |
amod |
developmental,stages |
R5733 |
T12854 |
T12855 |
mark |
with,described |
R5734 |
T12855 |
T12844 |
advcl |
described,set |
R5735 |
T12856 |
T12857 |
det |
all,genotypes |
R5736 |
T12857 |
T12855 |
nsubj |
genotypes,described |
R5737 |
T12858 |
T12855 |
prep |
throughout,described |
R5738 |
T12859 |
T12860 |
det |
the,study |
R5739 |
T12860 |
T12858 |
pobj |
study,throughout |
R5740 |
T12861 |
T12844 |
punct |
.,set |
R5741 |
T13099 |
T13100 |
amod |
Transcriptional,transactivation |
R5742 |
T13100 |
T13101 |
compound |
transactivation,assays |
R5743 |
T13103 |
T13104 |
det |
The,plasmids |
R5744 |
T13104 |
T13106 |
nsubjpass |
plasmids,used |
R5745 |
T13105 |
T13104 |
amod |
following,plasmids |
R5746 |
T13107 |
T13106 |
auxpass |
were,used |
R5747 |
T13108 |
T13106 |
prep |
for,used |
R5748 |
T13109 |
T13110 |
amod |
transcriptional,assays |
R5749 |
T13110 |
T13108 |
pobj |
assays,for |
R5750 |
T13111 |
T13110 |
compound |
transactivation,assays |
R5751 |
T13112 |
T13106 |
punct |
: ,used |
R5752 |
T13113 |
T13114 |
compound |
pRc,RSV |
R5753 |
T13114 |
T13106 |
dobj |
RSV,used |
R5754 |
T13115 |
T13114 |
punct |
/,RSV |
R5755 |
T13116 |
T13117 |
punct |
(,Invitrogen |
R5756 |
T13117 |
T13114 |
parataxis |
Invitrogen,RSV |
R5757 |
T13118 |
T13117 |
punct |
", ",Invitrogen |
R5758 |
T13119 |
T13117 |
npadvmod |
Carlsbad,Invitrogen |
R5759 |
T13120 |
T13117 |
punct |
", ",Invitrogen |
R5760 |
T13121 |
T13117 |
npadvmod |
California,Invitrogen |
R5761 |
T13122 |
T13117 |
punct |
", ",Invitrogen |
R5762 |
T13123 |
T13124 |
compound |
United,States |
R5763 |
T13124 |
T13117 |
npadvmod |
States,Invitrogen |
R5764 |
T13125 |
T13117 |
punct |
),Invitrogen |
R5765 |
T13126 |
T13114 |
punct |
", ",RSV |
R5766 |
T13127 |
T13128 |
compound |
pRc,RSV |
R5767 |
T13128 |
T13130 |
compound |
RSV,Er81 |
R5768 |
T13129 |
T13128 |
punct |
/,RSV |
R5769 |
T13130 |
T13114 |
conj |
Er81,RSV |
R5770 |
T13131 |
T13130 |
punct |
-,Er81 |
R5771 |
T13132 |
T13130 |
punct |
", ",Er81 |
R5772 |
T13133 |
T13134 |
compound |
pRc,RSV |
R5773 |
T13134 |
T13136 |
compound |
RSV,Pea3 |
R5774 |
T13135 |
T13134 |
punct |
/,RSV |
R5775 |
T13136 |
T13130 |
conj |
Pea3,Er81 |
R5776 |
T13137 |
T13136 |
punct |
-,Pea3 |
R5777 |
T13138 |
T13136 |
compound |
EWS,Pea3 |
R5778 |
T13139 |
T13136 |
punct |
-,Pea3 |
R5779 |
T13140 |
T13136 |
punct |
", ",Pea3 |
R5780 |
T13141 |
T13142 |
compound |
pTP,5xETS |
R5781 |
T13142 |
T13136 |
conj |
5xETS,Pea3 |
R5782 |
T13143 |
T13142 |
punct |
-,5xETS |
R5783 |
T13144 |
T13142 |
punct |
", ",5xETS |
R5784 |
T13145 |
T13142 |
cc |
and,5xETS |
R5785 |
T13146 |
T13147 |
compound |
pTP,5xETSmut |
R5786 |
T13147 |
T13142 |
conj |
5xETSmut,5xETS |
R5787 |
T13148 |
T13147 |
punct |
-,5xETSmut |
R5788 |
T13149 |
T13106 |
punct |
.,used |
R5789 |
T13151 |
T13152 |
compound |
pRc,RSV |
R5790 |
T13152 |
T13154 |
compound |
RSV,Er81 |
R5791 |
T13153 |
T13152 |
punct |
/,RSV |
R5792 |
T13154 |
T13156 |
nsubjpass |
Er81,obtained |
R5793 |
T13155 |
T13154 |
punct |
-,Er81 |
R5794 |
T13157 |
T13154 |
cc |
and,Er81 |
R5795 |
T13158 |
T13159 |
compound |
pRc,RSV |
R5796 |
T13159 |
T13161 |
compound |
RSV,Pea3 |
R5797 |
T13160 |
T13159 |
punct |
/,RSV |
R5798 |
T13161 |
T13154 |
conj |
Pea3,Er81 |
R5799 |
T13162 |
T13161 |
punct |
-,Pea3 |
R5800 |
T13163 |
T13161 |
compound |
EWS,Pea3 |
R5801 |
T13164 |
T13161 |
punct |
-,Pea3 |
R5802 |
T13165 |
T13156 |
auxpass |
were,obtained |
R5803 |
T13166 |
T13156 |
prep |
by,obtained |
R5804 |
T13167 |
T13166 |
pobj |
insertion,by |
R5805 |
T13168 |
T13167 |
prep |
of,insertion |
R5806 |
T13169 |
T13170 |
det |
the,cDNAs |
R5807 |
T13170 |
T13168 |
pobj |
cDNAs,of |
R5808 |
T13171 |
T13170 |
prep |
for,cDNAs |
R5809 |
T13172 |
T13171 |
pobj |
Er81,for |
R5810 |
T13173 |
T13172 |
cc |
or,Er81 |
R5811 |
T13174 |
T13175 |
compound |
EWS,Pea3 |
R5812 |
T13175 |
T13172 |
conj |
Pea3,Er81 |
R5813 |
T13176 |
T13175 |
punct |
-,Pea3 |
R5814 |
T13177 |
T13178 |
punct |
(,gift |
R5815 |
T13178 |
T13175 |
parataxis |
gift,Pea3 |
R5816 |
T13179 |
T13178 |
prep |
from,gift |
R5817 |
T13180 |
T13181 |
compound |
J.,Hassell |
R5818 |
T13181 |
T13179 |
pobj |
Hassell,from |
R5819 |
T13182 |
T13181 |
compound |
A.,Hassell |
R5820 |
T13183 |
T13178 |
punct |
),gift |
R5821 |
T13184 |
T13167 |
prep |
into,insertion |
R5822 |
T13185 |
T13186 |
compound |
pRc,RSV |
R5823 |
T13186 |
T13184 |
pobj |
RSV,into |
R5824 |
T13187 |
T13186 |
punct |
/,RSV |
R5825 |
T13188 |
T13156 |
punct |
.,obtained |
R5826 |
T13190 |
T13191 |
compound |
pTP,5xETS |
R5827 |
T13191 |
T13193 |
nsubjpass |
5xETS,constructed |
R5828 |
T13192 |
T13191 |
punct |
-,5xETS |
R5829 |
T13194 |
T13193 |
auxpass |
was,constructed |
R5830 |
T13195 |
T13193 |
prep |
by,constructed |
R5831 |
T13196 |
T13195 |
pcomp |
inserting,by |
R5832 |
T13197 |
T13198 |
det |
a,cassette |
R5833 |
T13198 |
T13196 |
dobj |
cassette,inserting |
R5834 |
T13199 |
T13198 |
prep |
of,cassette |
R5835 |
T13200 |
T13201 |
nummod |
five,copies |
R5836 |
T13201 |
T13199 |
pobj |
copies,of |
R5837 |
T13202 |
T13201 |
amod |
repetitive,copies |
R5838 |
T13203 |
T13201 |
prep |
of,copies |
R5839 |
T13204 |
T13205 |
amod |
high,affinity |
R5840 |
T13205 |
T13207 |
compound |
affinity,sites |
R5841 |
T13206 |
T13205 |
punct |
-,affinity |
R5842 |
T13207 |
T13203 |
pobj |
sites,of |
R5843 |
T13208 |
T13207 |
compound |
Pea3,sites |
R5844 |
T13209 |
T13207 |
compound |
binding,sites |
R5845 |
T13210 |
T13211 |
punct |
(,3 |
R5846 |
T13211 |
T13207 |
parataxis |
3,sites |
R5847 |
T13212 |
T13211 |
dep |
5,3 |
R5848 |
T13213 |
T13212 |
punct |
′,5 |
R5849 |
T13214 |
T13211 |
punct |
-,3 |
R5850 |
T13215 |
T13211 |
dep |
GCCGGAAGC,3 |
R5851 |
T13216 |
T13211 |
punct |
-,3 |
R5852 |
T13217 |
T13211 |
punct |
′,3 |
R5853 |
T13218 |
T13211 |
punct |
),3 |
R5854 |
T13219 |
T13220 |
punct |
[,19 |
R5855 |
T13220 |
T13207 |
parataxis |
19,sites |
R5856 |
T13221 |
T13220 |
nummod |
18,19 |
R5857 |
T13222 |
T13220 |
punct |
",",19 |
R5858 |
T13223 |
T13220 |
punct |
],19 |
R5859 |
T13224 |
T13196 |
prep |
into,inserting |
R5860 |
T13225 |
T13226 |
det |
a,version |
R5861 |
T13226 |
T13224 |
pobj |
version,into |
R5862 |
T13227 |
T13226 |
amod |
modified,version |
R5863 |
T13228 |
T13226 |
prep |
of,version |
R5864 |
T13229 |
T13230 |
compound |
pTK,Luc |
R5865 |
T13230 |
T13228 |
pobj |
Luc,of |
R5866 |
T13231 |
T13230 |
punct |
-,Luc |
R5867 |
T13232 |
T13193 |
punct |
.,constructed |
R5868 |
T13234 |
T13235 |
compound |
pTP,5xETSmut |
R5869 |
T13235 |
T13237 |
nsubjpass |
5xETSmut,generated |
R5870 |
T13236 |
T13235 |
punct |
-,5xETSmut |
R5871 |
T13238 |
T13237 |
auxpass |
was,generated |
R5872 |
T13239 |
T13237 |
prep |
as,generated |
R5873 |
T13240 |
T13241 |
compound |
pTP,5xETS |
R5874 |
T13241 |
T13239 |
pobj |
5xETS,as |
R5875 |
T13242 |
T13241 |
punct |
-,5xETS |
R5876 |
T13243 |
T13239 |
cc |
but,as |
R5877 |
T13244 |
T13239 |
conj |
using,as |
R5878 |
T13245 |
T13246 |
det |
a,complement |
R5879 |
T13246 |
T13244 |
dobj |
complement,using |
R5880 |
T13247 |
T13246 |
amod |
mutated,complement |
R5881 |
T13248 |
T13246 |
prep |
of,complement |
R5882 |
T13249 |
T13250 |
det |
the,sites |
R5883 |
T13250 |
T13248 |
pobj |
sites,of |
R5884 |
T13251 |
T13250 |
compound |
Pea3,sites |
R5885 |
T13252 |
T13250 |
compound |
binding,sites |
R5886 |
T13253 |
T13254 |
punct |
(,3 |
R5887 |
T13254 |
T13246 |
parataxis |
3,complement |
R5888 |
T13255 |
T13254 |
dep |
5,3 |
R5889 |
T13256 |
T13255 |
punct |
′,5 |
R5890 |
T13257 |
T13254 |
punct |
-,3 |
R5891 |
T13258 |
T13254 |
dep |
GCCTATGGC,3 |
R5892 |
T13259 |
T13254 |
punct |
-,3 |
R5893 |
T13260 |
T13254 |
punct |
′,3 |
R5894 |
T13261 |
T13254 |
punct |
),3 |
R5895 |
T13262 |
T13237 |
punct |
.,generated |
R5896 |
T13264 |
T13265 |
det |
A,plasmid |
R5897 |
T13265 |
T13267 |
nsubj |
plasmid,were |
R5898 |
T13266 |
T13265 |
compound |
control,plasmid |
R5899 |
T13268 |
T13269 |
aux |
to,normalize |
R5900 |
T13269 |
T13265 |
advcl |
normalize,plasmid |
R5901 |
T13270 |
T13269 |
prep |
for,normalize |
R5902 |
T13271 |
T13272 |
compound |
transfection,efficiency |
R5903 |
T13272 |
T13270 |
pobj |
efficiency,for |
R5904 |
T13273 |
T13274 |
punct |
(,placZ |
R5905 |
T13274 |
T13272 |
parataxis |
placZ,efficiency |
R5906 |
T13275 |
T13274 |
punct |
),placZ |
R5907 |
T13276 |
T13272 |
cc |
and,efficiency |
R5908 |
T13277 |
T13278 |
compound |
pTK,Luc |
R5909 |
T13278 |
T13272 |
conj |
Luc,efficiency |
R5910 |
T13279 |
T13278 |
punct |
-,Luc |
R5911 |
T13280 |
T13281 |
det |
a,gift |
R5912 |
T13281 |
T13267 |
attr |
gift,were |
R5913 |
T13282 |
T13281 |
prep |
from,gift |
R5914 |
T13283 |
T13284 |
compound |
D.,Kressler |
R5915 |
T13284 |
T13282 |
pobj |
Kressler,from |
R5916 |
T13285 |
T13267 |
punct |
.,were |
R5917 |
T13287 |
T13288 |
nmod |
COS,cells |
R5918 |
T13288 |
T13291 |
nsubjpass |
cells,co-transfected |
R5919 |
T13289 |
T13287 |
punct |
-,COS |
R5920 |
T13290 |
T13287 |
nummod |
7,COS |
R5921 |
T13292 |
T13291 |
auxpass |
were,co-transfected |
R5922 |
T13293 |
T13291 |
prep |
with,co-transfected |
R5923 |
T13294 |
T13295 |
quantmod |
1,1.2 |
R5924 |
T13295 |
T13297 |
nummod |
1.2,μg |
R5925 |
T13296 |
T13295 |
punct |
–,1.2 |
R5926 |
T13297 |
T13293 |
pobj |
μg,with |
R5927 |
T13298 |
T13297 |
prep |
of,μg |
R5928 |
T13299 |
T13300 |
amod |
total,DNA |
R5929 |
T13300 |
T13298 |
pobj |
DNA,of |
R5930 |
T13301 |
T13297 |
prep |
including,μg |
R5931 |
T13302 |
T13301 |
pobj |
one,including |
R5932 |
T13303 |
T13302 |
prep |
of,one |
R5933 |
T13304 |
T13305 |
det |
the,plasmids |
R5934 |
T13305 |
T13303 |
pobj |
plasmids,of |
R5935 |
T13306 |
T13305 |
compound |
effector,plasmids |
R5936 |
T13307 |
T13308 |
compound |
pRc,RSV |
R5937 |
T13308 |
T13302 |
appos |
RSV,one |
R5938 |
T13309 |
T13308 |
punct |
/,RSV |
R5939 |
T13310 |
T13308 |
punct |
-,RSV |
R5940 |
T13311 |
T13308 |
amod |
empty,RSV |
R5941 |
T13312 |
T13308 |
punct |
", ",RSV |
R5942 |
T13313 |
T13314 |
compound |
pRc,RSV |
R5943 |
T13314 |
T13316 |
compound |
RSV,Er81 |
R5944 |
T13315 |
T13314 |
punct |
/,RSV |
R5945 |
T13316 |
T13308 |
conj |
Er81,RSV |
R5946 |
T13317 |
T13316 |
punct |
-,Er81 |
R5947 |
T13318 |
T13316 |
punct |
", ",Er81 |
R5948 |
T13319 |
T13316 |
cc |
or,Er81 |
R5949 |
T13320 |
T13321 |
compound |
pRc,RSV |
R5950 |
T13321 |
T13323 |
compound |
RSV,Pea3 |
R5951 |
T13322 |
T13321 |
punct |
/,RSV |
R5952 |
T13323 |
T13316 |
conj |
Pea3,Er81 |
R5953 |
T13324 |
T13323 |
punct |
-,Pea3 |
R5954 |
T13325 |
T13323 |
compound |
EWS,Pea3 |
R5955 |
T13326 |
T13323 |
punct |
-,Pea3 |
R5956 |
T13327 |
T13302 |
punct |
;,one |
R5957 |
T13328 |
T13302 |
conj |
one,one |
R5958 |
T13329 |
T13328 |
prep |
of,one |
R5959 |
T13330 |
T13331 |
det |
the,plasmids |
R5960 |
T13331 |
T13329 |
pobj |
plasmids,of |
R5961 |
T13332 |
T13331 |
compound |
reporter,plasmids |
R5962 |
T13333 |
T13334 |
compound |
pTP,5xETS |
R5963 |
T13334 |
T13328 |
appos |
5xETS,one |
R5964 |
T13335 |
T13334 |
punct |
-,5xETS |
R5965 |
T13336 |
T13334 |
cc |
or,5xETS |
R5966 |
T13337 |
T13338 |
compound |
pTP,5xETSmut |
R5967 |
T13338 |
T13334 |
conj |
5xETSmut,5xETS |
R5968 |
T13339 |
T13338 |
punct |
-,5xETSmut |
R5969 |
T13340 |
T13328 |
punct |
;,one |
R5970 |
T13341 |
T13328 |
cc |
and,one |
R5971 |
T13342 |
T13328 |
conj |
placZ,one |
R5972 |
T13343 |
T13291 |
punct |
.,co-transfected |
R5973 |
T13345 |
T13346 |
nsubjpass |
Cells,harvested |
R5974 |
T13347 |
T13346 |
auxpass |
were,harvested |
R5975 |
T13348 |
T13346 |
prep |
after,harvested |
R5976 |
T13349 |
T13350 |
nummod |
25,h |
R5977 |
T13350 |
T13348 |
pobj |
h,after |
R5978 |
T13351 |
T13346 |
cc |
and,harvested |
R5979 |
T13352 |
T13346 |
conj |
processed,harvested |
R5980 |
T13353 |
T13352 |
prep |
for,processed |
R5981 |
T13354 |
T13353 |
pobj |
assays,for |
R5982 |
T13355 |
T13356 |
aux |
to,determine |
R5983 |
T13356 |
T13352 |
advcl |
determine,processed |
R5984 |
T13357 |
T13358 |
nmod |
luciferase,activity |
R5985 |
T13358 |
T13356 |
dobj |
activity,determine |
R5986 |
T13359 |
T13357 |
cc |
and,luciferase |
R5987 |
T13360 |
T13357 |
conj |
LacZ,luciferase |
R5988 |
T13361 |
T13362 |
mark |
as,described |
R5989 |
T13362 |
T13356 |
advcl |
described,determine |
R5990 |
T13363 |
T13362 |
advmod |
previously,described |
R5991 |
T13364 |
T13365 |
punct |
[,44 |
R5992 |
T13365 |
T13352 |
parataxis |
44,processed |
R5993 |
T13366 |
T13365 |
punct |
],44 |
R5994 |
T13367 |
T13346 |
punct |
.,harvested |
R5995 |
T13369 |
T13370 |
compound |
Luciferase,values |
R5996 |
T13370 |
T13371 |
nsubjpass |
values,referred |
R5997 |
T13372 |
T13370 |
acl |
normalized,values |
R5998 |
T13373 |
T13372 |
prep |
to,normalized |
R5999 |
T13374 |
T13375 |
compound |
LacZ,activity |
R6000 |
T13375 |
T13373 |
pobj |
activity,to |
R6001 |
T13376 |
T13371 |
auxpass |
are,referred |
R6002 |
T13377 |
T13371 |
prep |
to,referred |
R6003 |
T13378 |
T13371 |
prep |
as,referred |
R6004 |
T13379 |
T13380 |
compound |
luciferase,units |
R6005 |
T13380 |
T13378 |
pobj |
units,as |
R6006 |
T13381 |
T13371 |
punct |
.,referred |
R6013 |
T13917 |
T13918 |
advmod |
In,situ |
R6014 |
T13918 |
T13919 |
amod |
situ,hybridization |
R6015 |
T13920 |
T13919 |
cc |
and,hybridization |
R6016 |
T13921 |
T13919 |
conj |
immunohistochemistry,hybridization |
R6017 |
T13923 |
T13924 |
prep |
For,hybridized |
R6018 |
T13925 |
T13926 |
advmod |
in,situ |
R6019 |
T13926 |
T13927 |
amod |
situ,analysis |
R6020 |
T13927 |
T13923 |
pobj |
analysis,For |
R6021 |
T13928 |
T13927 |
compound |
hybridization,analysis |
R6022 |
T13929 |
T13924 |
punct |
", ",hybridized |
R6023 |
T13930 |
T13931 |
compound |
cryostat,sections |
R6024 |
T13931 |
T13924 |
nsubjpass |
sections,hybridized |
R6025 |
T13932 |
T13924 |
auxpass |
were,hybridized |
R6026 |
T13933 |
T13924 |
advcl |
using,hybridized |
R6027 |
T13934 |
T13935 |
npadvmod |
digoxigenin,labeled |
R6028 |
T13935 |
T13937 |
amod |
labeled,probes |
R6029 |
T13936 |
T13935 |
punct |
-,labeled |
R6030 |
T13937 |
T13933 |
dobj |
probes,using |
R6031 |
T13938 |
T13939 |
punct |
[,45 |
R6032 |
T13939 |
T13937 |
parataxis |
45,probes |
R6033 |
T13940 |
T13939 |
punct |
],45 |
R6034 |
T13941 |
T13937 |
acl |
directed,probes |
R6035 |
T13942 |
T13941 |
prep |
against,directed |
R6036 |
T13943 |
T13944 |
compound |
mouse,TrkA |
R6037 |
T13944 |
T13942 |
pobj |
TrkA,against |
R6038 |
T13945 |
T13944 |
cc |
or,TrkA |
R6039 |
T13946 |
T13944 |
conj |
TrkB,TrkA |
R6040 |
T13947 |
T13944 |
punct |
", ",TrkA |
R6041 |
T13948 |
T13944 |
cc |
or,TrkA |
R6042 |
T13949 |
T13950 |
compound |
rat,TrkC |
R6043 |
T13950 |
T13944 |
conj |
TrkC,TrkA |
R6044 |
T13951 |
T13952 |
punct |
(,gift |
R6045 |
T13952 |
T13950 |
parataxis |
gift,TrkC |
R6046 |
T13953 |
T13952 |
prep |
from,gift |
R6047 |
T13954 |
T13955 |
compound |
L.,Parada |
R6048 |
T13955 |
T13953 |
pobj |
Parada,from |
R6049 |
T13956 |
T13955 |
compound |
F.,Parada |
R6050 |
T13957 |
T13952 |
punct |
),gift |
R6051 |
T13958 |
T13924 |
punct |
.,hybridized |
R6052 |
T13960 |
T13961 |
nsubj |
Antibodies,were |
R6053 |
T13962 |
T13960 |
acl |
used,Antibodies |
R6054 |
T13963 |
T13962 |
prep |
in,used |
R6055 |
T13964 |
T13965 |
det |
this,study |
R6056 |
T13965 |
T13963 |
pobj |
study,in |
R6057 |
T13966 |
T13967 |
mark |
as,follows |
R6058 |
T13967 |
T13961 |
advcl |
follows,were |
R6059 |
T13968 |
T13961 |
punct |
: ,were |
R6060 |
T13969 |
T13970 |
compound |
rabbit,anti-Er81 |
R6061 |
T13970 |
T13961 |
dep |
anti-Er81,were |
R6062 |
T13971 |
T13972 |
punct |
[,14 |
R6063 |
T13972 |
T13970 |
parataxis |
14,anti-Er81 |
R6064 |
T13973 |
T13972 |
punct |
],14 |
R6065 |
T13974 |
T13970 |
punct |
", ",anti-Er81 |
R6066 |
T13975 |
T13970 |
conj |
rabbit,anti-Er81 |
R6067 |
T13976 |
T13975 |
amod |
anti-Pea3,rabbit |
R6068 |
T13977 |
T13978 |
punct |
[,14 |
R6069 |
T13978 |
T13975 |
parataxis |
14,rabbit |
R6070 |
T13979 |
T13978 |
punct |
],14 |
R6071 |
T13980 |
T13975 |
punct |
", ",rabbit |
R6072 |
T13981 |
T13975 |
conj |
rabbit,rabbit |
R6073 |
T13982 |
T13981 |
amod |
anti-PV,rabbit |
R6074 |
T13983 |
T13984 |
punct |
[,14 |
R6075 |
T13984 |
T13981 |
parataxis |
14,rabbit |
R6076 |
T13985 |
T13984 |
punct |
],14 |
R6077 |
T13986 |
T13981 |
punct |
", ",rabbit |
R6078 |
T13987 |
T13981 |
conj |
rabbit,rabbit |
R6079 |
T13988 |
T13987 |
amod |
anti-eGFP,rabbit |
R6080 |
T13989 |
T13990 |
punct |
(,Probes |
R6081 |
T13990 |
T13987 |
parataxis |
Probes,rabbit |
R6082 |
T13991 |
T13990 |
compound |
Molecular,Probes |
R6083 |
T13992 |
T13990 |
punct |
", ",Probes |
R6084 |
T13993 |
T13990 |
npadvmod |
Eugene,Probes |
R6085 |
T13994 |
T13990 |
punct |
", ",Probes |
R6086 |
T13995 |
T13990 |
npadvmod |
Oregon,Probes |
R6087 |
T13996 |
T13990 |
punct |
", ",Probes |
R6088 |
T13997 |
T13998 |
compound |
United,States |
R6089 |
T13998 |
T13990 |
npadvmod |
States,Probes |
R6090 |
T13999 |
T13990 |
punct |
),Probes |
R6091 |
T14000 |
T13987 |
punct |
", ",rabbit |
R6092 |
T14001 |
T13987 |
conj |
rabbit,rabbit |
R6093 |
T14002 |
T14001 |
amod |
anti-Calbindin,rabbit |
R6094 |
T14003 |
T14001 |
punct |
", ",rabbit |
R6095 |
T14004 |
T14001 |
conj |
rabbit,rabbit |
R6096 |
T14005 |
T14004 |
amod |
anti-Calretinin,rabbit |
R6097 |
T14006 |
T14007 |
punct |
(,Swant |
R6098 |
T14007 |
T14004 |
parataxis |
Swant,rabbit |
R6099 |
T14008 |
T14007 |
punct |
", ",Swant |
R6100 |
T14009 |
T14007 |
npadvmod |
Bellinzona,Swant |
R6101 |
T14010 |
T14007 |
punct |
", ",Swant |
R6102 |
T14011 |
T14007 |
npadvmod |
Switzerland,Swant |
R6103 |
T14012 |
T14007 |
punct |
),Swant |
R6104 |
T14013 |
T14004 |
punct |
", ",rabbit |
R6105 |
T14014 |
T14004 |
conj |
rabbit,rabbit |
R6106 |
T14015 |
T14014 |
amod |
anti-CGRP,rabbit |
R6107 |
T14016 |
T14017 |
punct |
(,Chemicon |
R6108 |
T14017 |
T14014 |
parataxis |
Chemicon,rabbit |
R6109 |
T14018 |
T14017 |
punct |
", ",Chemicon |
R6110 |
T14019 |
T14017 |
npadvmod |
Temecula,Chemicon |
R6111 |
T14020 |
T14017 |
punct |
", ",Chemicon |
R6112 |
T14021 |
T14017 |
npadvmod |
California,Chemicon |
R6113 |
T14022 |
T14017 |
punct |
", ",Chemicon |
R6114 |
T14023 |
T14024 |
compound |
United,States |
R6115 |
T14024 |
T14017 |
npadvmod |
States,Chemicon |
R6116 |
T14025 |
T14017 |
punct |
),Chemicon |
R6117 |
T14026 |
T14014 |
punct |
", ",rabbit |
R6118 |
T14027 |
T14014 |
conj |
rabbit,rabbit |
R6119 |
T14028 |
T14027 |
amod |
anti-vGlut1,rabbit |
R6120 |
T14029 |
T14030 |
punct |
(,Systems |
R6121 |
T14030 |
T14027 |
parataxis |
Systems,rabbit |
R6122 |
T14031 |
T14030 |
compound |
Synaptic,Systems |
R6123 |
T14032 |
T14030 |
punct |
", ",Systems |
R6124 |
T14033 |
T14030 |
npadvmod |
Goettingen,Systems |
R6125 |
T14034 |
T14030 |
punct |
", ",Systems |
R6126 |
T14035 |
T14030 |
npadvmod |
Germany,Systems |
R6127 |
T14036 |
T14030 |
punct |
),Systems |
R6128 |
T14037 |
T14027 |
punct |
", ",rabbit |
R6129 |
T14038 |
T14027 |
conj |
rabbit,rabbit |
R6130 |
T14039 |
T14038 |
amod |
anti-Brn3a,rabbit |
R6131 |
T14040 |
T14041 |
punct |
(,gift |
R6132 |
T14041 |
T14038 |
parataxis |
gift,rabbit |
R6133 |
T14042 |
T14041 |
prep |
from,gift |
R6134 |
T14043 |
T14044 |
compound |
E.,Turner |
R6135 |
T14044 |
T14042 |
pobj |
Turner,from |
R6136 |
T14045 |
T14041 |
punct |
),gift |
R6137 |
T14046 |
T14038 |
punct |
", ",rabbit |
R6138 |
T14047 |
T14048 |
nmod |
rabbit,p75 |
R6139 |
T14048 |
T14038 |
conj |
p75,rabbit |
R6140 |
T14049 |
T14048 |
amod |
anti-TrkA,p75 |
R6141 |
T14050 |
T14048 |
cc |
and,p75 |
R6142 |
T14051 |
T14048 |
punct |
-,p75 |
R6143 |
T14052 |
T14053 |
punct |
(,gift |
R6144 |
T14053 |
T14048 |
parataxis |
gift,p75 |
R6145 |
T14054 |
T14053 |
prep |
from,gift |
R6146 |
T14055 |
T14056 |
compound |
L.,Reichardt |
R6147 |
T14056 |
T14054 |
pobj |
Reichardt,from |
R6148 |
T14057 |
T14056 |
compound |
F.,Reichardt |
R6149 |
T14058 |
T14053 |
punct |
),gift |
R6150 |
T14059 |
T14048 |
punct |
", ",p75 |
R6151 |
T14060 |
T14048 |
conj |
rabbit,p75 |
R6152 |
T14061 |
T14060 |
amod |
anti-Runx3,rabbit |
R6153 |
T14062 |
T14063 |
punct |
(,Kramer |
R6154 |
T14063 |
T14060 |
parataxis |
Kramer,rabbit |
R6155 |
T14064 |
T14063 |
cc |
and,Kramer |
R6156 |
T14065 |
T14063 |
conj |
Arber,Kramer |
R6157 |
T14066 |
T14063 |
punct |
", ",Kramer |
R6158 |
T14067 |
T14068 |
amod |
unpublished,reagent |
R6159 |
T14068 |
T14063 |
npadvmod |
reagent,Kramer |
R6160 |
T14069 |
T14063 |
punct |
),Kramer |
R6161 |
T14070 |
T14060 |
punct |
", ",rabbit |
R6162 |
T14071 |
T14060 |
conj |
rabbit,rabbit |
R6163 |
T14072 |
T14071 |
amod |
anti-Rhodamine,rabbit |
R6164 |
T14073 |
T14074 |
punct |
(,Probes |
R6165 |
T14074 |
T14071 |
parataxis |
Probes,rabbit |
R6166 |
T14075 |
T14074 |
compound |
Molecular,Probes |
R6167 |
T14076 |
T14074 |
punct |
),Probes |
R6168 |
T14077 |
T14071 |
punct |
", ",rabbit |
R6169 |
T14078 |
T14071 |
conj |
mouse,rabbit |
R6170 |
T14079 |
T14078 |
amod |
anti-neurofilament,mouse |
R6171 |
T14080 |
T14081 |
punct |
(,Collection |
R6172 |
T14081 |
T14078 |
parataxis |
Collection,mouse |
R6173 |
T14082 |
T14081 |
compound |
American,Collection |
R6174 |
T14083 |
T14081 |
compound |
Type,Collection |
R6175 |
T14084 |
T14081 |
compound |
Culture,Collection |
R6176 |
T14085 |
T14081 |
punct |
", ",Collection |
R6177 |
T14086 |
T14081 |
npadvmod |
Manassas,Collection |
R6178 |
T14087 |
T14081 |
punct |
", ",Collection |
R6179 |
T14088 |
T14081 |
npadvmod |
Virginia,Collection |
R6180 |
T14089 |
T14081 |
punct |
", ",Collection |
R6181 |
T14090 |
T14091 |
compound |
United,States |
R6182 |
T14091 |
T14081 |
npadvmod |
States,Collection |
R6183 |
T14092 |
T14081 |
punct |
),Collection |
R6184 |
T14093 |
T14078 |
punct |
", ",mouse |
R6185 |
T14094 |
T14078 |
conj |
sheep,mouse |
R6186 |
T14095 |
T14094 |
amod |
anti-eGFP,sheep |
R6187 |
T14096 |
T14097 |
punct |
(,Biogenesis |
R6188 |
T14097 |
T14094 |
parataxis |
Biogenesis,sheep |
R6189 |
T14098 |
T14097 |
punct |
", ",Biogenesis |
R6190 |
T14099 |
T14097 |
npadvmod |
Poole,Biogenesis |
R6191 |
T14100 |
T14097 |
punct |
", ",Biogenesis |
R6192 |
T14101 |
T14102 |
compound |
United,Kingdom |
R6193 |
T14102 |
T14097 |
npadvmod |
Kingdom,Biogenesis |
R6194 |
T14103 |
T14097 |
punct |
),Biogenesis |
R6195 |
T14104 |
T14094 |
punct |
", ",sheep |
R6196 |
T14105 |
T14094 |
conj |
goat,sheep |
R6197 |
T14106 |
T14105 |
amod |
anti-LacZ,goat |
R6198 |
T14107 |
T14108 |
punct |
[,14 |
R6199 |
T14108 |
T14105 |
parataxis |
14,goat |
R6200 |
T14109 |
T14108 |
punct |
],14 |
R6201 |
T14110 |
T14105 |
punct |
", ",goat |
R6202 |
T14111 |
T14105 |
conj |
goat,goat |
R6203 |
T14112 |
T14111 |
amod |
anti-TrkC,goat |
R6204 |
T14113 |
T14114 |
punct |
(,gift |
R6205 |
T14114 |
T14111 |
parataxis |
gift,goat |
R6206 |
T14115 |
T14114 |
prep |
from,gift |
R6207 |
T14116 |
T14117 |
compound |
L.,Reichardt |
R6208 |
T14117 |
T14115 |
pobj |
Reichardt,from |
R6209 |
T14118 |
T14117 |
compound |
F.,Reichardt |
R6210 |
T14119 |
T14114 |
punct |
),gift |
R6211 |
T14120 |
T14111 |
punct |
", ",goat |
R6212 |
T14121 |
T14111 |
cc |
and,goat |
R6213 |
T14122 |
T14123 |
compound |
guinea,pig |
R6214 |
T14123 |
T14111 |
conj |
pig,goat |
R6215 |
T14124 |
T14123 |
amod |
anti-Isl1,pig |
R6216 |
T14125 |
T14126 |
punct |
[,14 |
R6217 |
T14126 |
T14123 |
parataxis |
14,pig |
R6218 |
T14127 |
T14126 |
punct |
],14 |
R6219 |
T14128 |
T13961 |
punct |
.,were |
R6220 |
T14130 |
T14131 |
amod |
Terminal,transferase |
R6221 |
T14131 |
T14133 |
npadvmod |
transferase,mediated |
R6222 |
T14132 |
T14131 |
compound |
deoxynucleotidyl,transferase |
R6223 |
T14133 |
T14135 |
amod |
mediated,labeling |
R6224 |
T14134 |
T14133 |
punct |
-,mediated |
R6225 |
T14135 |
T14140 |
nsubjpass |
labeling,performed |
R6226 |
T14136 |
T14135 |
amod |
biotinylated,labeling |
R6227 |
T14137 |
T14135 |
compound |
UTP,labeling |
R6228 |
T14138 |
T14135 |
compound |
nick,labeling |
R6229 |
T14139 |
T14135 |
compound |
end,labeling |
R6230 |
T14141 |
T14135 |
punct |
(,labeling |
R6231 |
T14142 |
T14135 |
appos |
TUNEL,labeling |
R6232 |
T14143 |
T14135 |
punct |
),labeling |
R6233 |
T14144 |
T14145 |
aux |
to,detect |
R6234 |
T14145 |
T14135 |
advcl |
detect,labeling |
R6235 |
T14146 |
T14147 |
amod |
apoptotic,cells |
R6236 |
T14147 |
T14145 |
dobj |
cells,detect |
R6237 |
T14148 |
T14145 |
prep |
in,detect |
R6238 |
T14149 |
T14150 |
compound |
E13.5,DRG |
R6239 |
T14150 |
T14148 |
pobj |
DRG,in |
R6240 |
T14151 |
T14145 |
prep |
on,detect |
R6241 |
T14152 |
T14153 |
compound |
cryostat,sections |
R6242 |
T14153 |
T14151 |
pobj |
sections,on |
R6243 |
T14154 |
T14140 |
auxpass |
was,performed |
R6244 |
T14155 |
T14156 |
mark |
as,described |
R6245 |
T14156 |
T14140 |
advcl |
described,performed |
R6246 |
T14157 |
T14156 |
agent |
by,described |
R6247 |
T14158 |
T14159 |
det |
the,manufacturer |
R6248 |
T14159 |
T14157 |
pobj |
manufacturer,by |
R6249 |
T14160 |
T14161 |
punct |
(,Roche |
R6250 |
T14161 |
T14159 |
parataxis |
Roche,manufacturer |
R6251 |
T14162 |
T14161 |
punct |
", ",Roche |
R6252 |
T14163 |
T14161 |
npadvmod |
Basel,Roche |
R6253 |
T14164 |
T14161 |
punct |
", ",Roche |
R6254 |
T14165 |
T14161 |
npadvmod |
Switzerland,Roche |
R6255 |
T14166 |
T14161 |
punct |
),Roche |
R6256 |
T14167 |
T14140 |
punct |
.,performed |
R6257 |
T14169 |
T14170 |
amod |
Quantitative,analysis |
R6258 |
T14170 |
T14171 |
nsubjpass |
analysis,performed |
R6259 |
T14172 |
T14170 |
prep |
of,analysis |
R6260 |
T14173 |
T14174 |
nmod |
TUNEL,cells |
R6261 |
T14174 |
T14172 |
pobj |
cells,of |
R6262 |
T14175 |
T14173 |
punct |
+,TUNEL |
R6263 |
T14176 |
T14174 |
compound |
DRG,cells |
R6264 |
T14177 |
T14171 |
auxpass |
was,performed |
R6265 |
T14178 |
T14179 |
advmod |
essentially,described |
R6266 |
T14179 |
T14171 |
advcl |
described,performed |
R6267 |
T14180 |
T14179 |
mark |
as,described |
R6268 |
T14181 |
T14182 |
punct |
[,27 |
R6269 |
T14182 |
T14179 |
parataxis |
27,described |
R6270 |
T14183 |
T14182 |
punct |
],27 |
R6271 |
T14184 |
T14171 |
punct |
.,performed |
R6272 |
T14186 |
T14187 |
compound |
BrdU,experiments |
R6273 |
T14187 |
T14191 |
nsubjpass |
experiments,performed |
R6274 |
T14188 |
T14189 |
compound |
pulse,chase |
R6275 |
T14189 |
T14187 |
compound |
chase,experiments |
R6276 |
T14190 |
T14189 |
punct |
-,chase |
R6277 |
T14192 |
T14187 |
cc |
and,experiments |
R6278 |
T14193 |
T14194 |
compound |
LacZ,stainings |
R6279 |
T14194 |
T14187 |
conj |
stainings,experiments |
R6280 |
T14195 |
T14194 |
compound |
wholemount,stainings |
R6281 |
T14196 |
T14191 |
auxpass |
were,performed |
R6282 |
T14197 |
T14198 |
mark |
as,described |
R6283 |
T14198 |
T14191 |
advcl |
described,performed |
R6284 |
T14199 |
T14198 |
advmod |
previously,described |
R6285 |
T14200 |
T14201 |
punct |
[,46 |
R6286 |
T14201 |
T14198 |
parataxis |
46,described |
R6287 |
T14202 |
T14201 |
punct |
],46 |
R6288 |
T14203 |
T14191 |
punct |
.,performed |
R6289 |
T14205 |
T14206 |
mark |
For,visualize |
R6290 |
T14206 |
T14211 |
advcl |
visualize,injected |
R6291 |
T14207 |
T14208 |
amod |
anterograde,experiments |
R6292 |
T14208 |
T14206 |
nsubj |
experiments,visualize |
R6293 |
T14209 |
T14208 |
compound |
tracing,experiments |
R6294 |
T14210 |
T14206 |
aux |
to,visualize |
R6295 |
T14212 |
T14206 |
dobj |
projections,visualize |
R6296 |
T14213 |
T14212 |
prep |
of,projections |
R6297 |
T14214 |
T14215 |
amod |
sensory,neurons |
R6298 |
T14215 |
T14213 |
pobj |
neurons,of |
R6299 |
T14216 |
T14211 |
punct |
", ",injected |
R6300 |
T14217 |
T14218 |
npadvmod |
rhodamine,conjugated |
R6301 |
T14218 |
T14220 |
amod |
conjugated,dextran |
R6302 |
T14219 |
T14218 |
punct |
-,conjugated |
R6303 |
T14220 |
T14211 |
nsubjpass |
dextran,injected |
R6304 |
T14221 |
T14222 |
punct |
(,Probes |
R6305 |
T14222 |
T14220 |
parataxis |
Probes,dextran |
R6306 |
T14223 |
T14222 |
compound |
Molecular,Probes |
R6307 |
T14224 |
T14222 |
punct |
),Probes |
R6308 |
T14225 |
T14211 |
auxpass |
was,injected |
R6309 |
T14226 |
T14211 |
prep |
into,injected |
R6310 |
T14227 |
T14228 |
amod |
single,DRG |
R6311 |
T14228 |
T14226 |
pobj |
DRG,into |
R6312 |
T14229 |
T14228 |
nmod |
lumbar,DRG |
R6313 |
T14230 |
T14231 |
punct |
(,L3 |
R6314 |
T14231 |
T14228 |
parataxis |
L3,DRG |
R6315 |
T14232 |
T14231 |
punct |
),L3 |
R6316 |
T14233 |
T14211 |
prep |
at,injected |
R6317 |
T14234 |
T14233 |
pobj |
E13.5,at |
R6318 |
T14235 |
T14211 |
cc |
or,injected |
R6319 |
T14236 |
T14211 |
conj |
applied,injected |
R6320 |
T14237 |
T14236 |
prep |
to,applied |
R6321 |
T14238 |
T14239 |
amod |
whole,roots |
R6322 |
T14239 |
T14237 |
pobj |
roots,to |
R6323 |
T14240 |
T14239 |
amod |
lumbar,roots |
R6324 |
T14241 |
T14239 |
amod |
dorsal,roots |
R6325 |
T14242 |
T14243 |
punct |
(,L3 |
R6326 |
T14243 |
T14239 |
parataxis |
L3,roots |
R6327 |
T14244 |
T14243 |
punct |
),L3 |
R6328 |
T14245 |
T14236 |
prep |
at,applied |
R6329 |
T14246 |
T14247 |
amod |
postnatal,day |
R6330 |
T14247 |
T14245 |
pobj |
day,at |
R6331 |
T14248 |
T14247 |
punct |
(,day |
R6332 |
T14249 |
T14247 |
appos |
P,day |
R6333 |
T14250 |
T14247 |
punct |
),day |
R6334 |
T14251 |
T14247 |
nummod |
5,day |
R6335 |
T14252 |
T14236 |
advcl |
using,applied |
R6336 |
T14253 |
T14254 |
compound |
glass,capillaries |
R6337 |
T14254 |
T14252 |
dobj |
capillaries,using |
R6338 |
T14255 |
T14211 |
punct |
.,injected |
R6339 |
T14257 |
T14258 |
prep |
After,incubated |
R6340 |
T14259 |
T14257 |
pobj |
injection,After |
R6341 |
T14260 |
T14258 |
punct |
", ",incubated |
R6342 |
T14261 |
T14258 |
nsubjpass |
animals,incubated |
R6343 |
T14262 |
T14258 |
auxpass |
were,incubated |
R6344 |
T14263 |
T14258 |
prep |
for,incubated |
R6345 |
T14264 |
T14265 |
quantmod |
2,3 |
R6346 |
T14265 |
T14267 |
nummod |
3,h |
R6347 |
T14266 |
T14265 |
punct |
–,3 |
R6348 |
T14267 |
T14263 |
pobj |
h,for |
R6349 |
T14268 |
T14269 |
punct |
(,E13.5 |
R6350 |
T14269 |
T14267 |
parataxis |
E13.5,h |
R6351 |
T14270 |
T14269 |
punct |
),E13.5 |
R6352 |
T14271 |
T14263 |
cc |
or,for |
R6353 |
T14272 |
T14263 |
conj |
overnight,for |
R6354 |
T14273 |
T14274 |
punct |
(,P5 |
R6355 |
T14274 |
T14272 |
parataxis |
P5,overnight |
R6356 |
T14275 |
T14274 |
punct |
),P5 |
R6357 |
T14276 |
T14258 |
punct |
.,incubated |
R6358 |
T14278 |
T14279 |
compound |
Cryostat,sections |
R6359 |
T14279 |
T14280 |
nsubjpass |
sections,processed |
R6360 |
T14281 |
T14280 |
auxpass |
were,processed |
R6361 |
T14282 |
T14280 |
prep |
for,processed |
R6362 |
T14283 |
T14282 |
pobj |
immunohistochemistry,for |
R6363 |
T14284 |
T14285 |
mark |
as,described |
R6364 |
T14285 |
T14280 |
advcl |
described,processed |
R6365 |
T14286 |
T14287 |
punct |
[,14 |
R6366 |
T14287 |
T14285 |
parataxis |
14,described |
R6367 |
T14288 |
T14287 |
punct |
],14 |
R6368 |
T14289 |
T14280 |
advcl |
using,processed |
R6369 |
T14290 |
T14291 |
npadvmod |
fluorophore,conjugated |
R6370 |
T14291 |
T14293 |
amod |
conjugated,antibodies |
R6371 |
T14292 |
T14291 |
punct |
-,conjugated |
R6372 |
T14293 |
T14289 |
dobj |
antibodies,using |
R6373 |
T14294 |
T14293 |
amod |
secondary,antibodies |
R6374 |
T14295 |
T14296 |
punct |
(,Probes |
R6375 |
T14296 |
T14293 |
parataxis |
Probes,antibodies |
R6376 |
T14297 |
T14296 |
dep |
1,Probes |
R6377 |
T14298 |
T14299 |
punct |
:,"1,000" |
R6378 |
T14299 |
T14297 |
prep |
"1,000",1 |
R6379 |
T14300 |
T14296 |
punct |
", ",Probes |
R6380 |
T14301 |
T14296 |
compound |
Molecular,Probes |
R6381 |
T14302 |
T14296 |
punct |
),Probes |
R6382 |
T14303 |
T14280 |
punct |
.,processed |
R6383 |
T14305 |
T14306 |
nsubjpass |
Images,collected |
R6384 |
T14307 |
T14306 |
auxpass |
were,collected |
R6385 |
T14308 |
T14306 |
prep |
on,collected |
R6386 |
T14309 |
T14310 |
det |
an,microscope |
R6387 |
T14310 |
T14308 |
pobj |
microscope,on |
R6388 |
T14311 |
T14310 |
nmod |
Olympus,microscope |
R6389 |
T14312 |
T14313 |
punct |
(,Tokyo |
R6390 |
T14313 |
T14311 |
parataxis |
Tokyo,Olympus |
R6391 |
T14314 |
T14313 |
punct |
", ",Tokyo |
R6392 |
T14315 |
T14313 |
npadvmod |
Japan,Tokyo |
R6393 |
T14316 |
T14313 |
punct |
),Tokyo |
R6394 |
T14317 |
T14310 |
amod |
confocal,microscope |
R6395 |
T14318 |
T14306 |
punct |
.,collected |
R6396 |
T14320 |
T14321 |
nsubjpass |
Images,collected |
R6397 |
T14322 |
T14320 |
prep |
from,Images |
R6398 |
T14323 |
T14324 |
nmod |
in,situ |
R6399 |
T14324 |
T14325 |
nmod |
situ,experiments |
R6400 |
T14325 |
T14322 |
pobj |
experiments,from |
R6401 |
T14326 |
T14325 |
compound |
hybridization,experiments |
R6402 |
T14327 |
T14321 |
auxpass |
were,collected |
R6403 |
T14328 |
T14321 |
prep |
with,collected |
R6404 |
T14329 |
T14330 |
det |
an,camera |
R6405 |
T14330 |
T14328 |
pobj |
camera,with |
R6406 |
T14331 |
T14332 |
compound |
RT,SPOT |
R6407 |
T14332 |
T14330 |
compound |
SPOT,camera |
R6408 |
T14333 |
T14332 |
punct |
-,SPOT |
R6409 |
T14334 |
T14335 |
punct |
(,Instruments |
R6410 |
T14335 |
T14330 |
parataxis |
Instruments,camera |
R6411 |
T14336 |
T14335 |
amod |
Diagnostic,Instruments |
R6412 |
T14337 |
T14335 |
punct |
", ",Instruments |
R6413 |
T14338 |
T14339 |
compound |
Sterling,Heights |
R6414 |
T14339 |
T14335 |
npadvmod |
Heights,Instruments |
R6415 |
T14340 |
T14335 |
punct |
", ",Instruments |
R6416 |
T14341 |
T14335 |
npadvmod |
Michigan,Instruments |
R6417 |
T14342 |
T14335 |
punct |
", ",Instruments |
R6418 |
T14343 |
T14344 |
compound |
United,States |
R6419 |
T14344 |
T14335 |
npadvmod |
States,Instruments |
R6420 |
T14345 |
T14335 |
punct |
),Instruments |
R6421 |
T14346 |
T14321 |
punct |
", ",collected |
R6422 |
T14347 |
T14321 |
cc |
and,collected |
R6423 |
T14348 |
T14349 |
nmod |
Corel,Paint |
R6424 |
T14349 |
T14360 |
nsubjpass |
Paint,used |
R6425 |
T14350 |
T14351 |
punct |
(,Prairie |
R6426 |
T14351 |
T14348 |
parataxis |
Prairie,Corel |
R6427 |
T14352 |
T14351 |
compound |
Eden,Prairie |
R6428 |
T14353 |
T14351 |
punct |
", ",Prairie |
R6429 |
T14354 |
T14351 |
npadvmod |
Minnesota,Prairie |
R6430 |
T14355 |
T14351 |
punct |
", ",Prairie |
R6431 |
T14356 |
T14357 |
compound |
United,States |
R6432 |
T14357 |
T14351 |
npadvmod |
States,Prairie |
R6433 |
T14358 |
T14351 |
punct |
),Prairie |
R6434 |
T14359 |
T14349 |
compound |
Photo,Paint |
R6435 |
T14360 |
T14321 |
conj |
used,collected |
R6436 |
T14361 |
T14349 |
nummod |
10.0,Paint |
R6437 |
T14362 |
T14360 |
auxpass |
was,used |
R6438 |
T14363 |
T14360 |
prep |
for,used |
R6439 |
T14364 |
T14365 |
amod |
digital,processing |
R6440 |
T14365 |
T14363 |
pobj |
processing,for |
R6441 |
T14366 |
T14365 |
prep |
of,processing |
R6442 |
T14367 |
T14366 |
pobj |
images,of |
R6443 |
T14368 |
T14360 |
punct |
.,used |
R6444 |
T14469 |
T14470 |
advmod |
In,vitro |
R6445 |
T14470 |
T14471 |
amod |
vitro,cultures |
R6446 |
T14472 |
T14471 |
prep |
of,cultures |
R6447 |
T14473 |
T14472 |
pobj |
DRG,of |
R6448 |
T14475 |
T14476 |
amod |
Individual,DRG |
R6449 |
T14476 |
T14478 |
nsubjpass |
DRG,dissected |
R6450 |
T14477 |
T14476 |
compound |
lumbar,DRG |
R6451 |
T14479 |
T14478 |
auxpass |
were,dissected |
R6452 |
T14480 |
T14478 |
prep |
from,dissected |
R6453 |
T14481 |
T14482 |
nmod |
E13.5,embryos |
R6454 |
T14482 |
T14480 |
pobj |
embryos,from |
R6455 |
T14483 |
T14481 |
cc |
or,E13.5 |
R6456 |
T14484 |
T14481 |
conj |
E14.5,E13.5 |
R6457 |
T14485 |
T14478 |
cc |
and,dissected |
R6458 |
T14486 |
T14478 |
conj |
placed,dissected |
R6459 |
T14487 |
T14486 |
prep |
on,placed |
R6460 |
T14488 |
T14489 |
npadvmod |
Matrigel,coated |
R6461 |
T14489 |
T14502 |
amod |
coated,coverslips |
R6462 |
T14490 |
T14491 |
punct |
(,Biosciences |
R6463 |
T14491 |
T14488 |
parataxis |
Biosciences,Matrigel |
R6464 |
T14492 |
T14491 |
compound |
BD,Biosciences |
R6465 |
T14493 |
T14491 |
punct |
", ",Biosciences |
R6466 |
T14494 |
T14495 |
compound |
San,Jose |
R6467 |
T14495 |
T14491 |
npadvmod |
Jose,Biosciences |
R6468 |
T14496 |
T14491 |
punct |
", ",Biosciences |
R6469 |
T14497 |
T14491 |
npadvmod |
California,Biosciences |
R6470 |
T14498 |
T14491 |
punct |
", ",Biosciences |
R6471 |
T14499 |
T14500 |
compound |
United,States |
R6472 |
T14500 |
T14491 |
npadvmod |
States,Biosciences |
R6473 |
T14501 |
T14491 |
punct |
),Biosciences |
R6474 |
T14502 |
T14487 |
pobj |
coverslips,on |
R6475 |
T14503 |
T14502 |
prep |
in,coverslips |
R6476 |
T14504 |
T14505 |
compound |
DMEM,F12 |
R6477 |
T14505 |
T14503 |
pobj |
F12,in |
R6478 |
T14506 |
T14505 |
punct |
/,F12 |
R6479 |
T14507 |
T14508 |
punct |
(,Gibco |
R6480 |
T14508 |
T14505 |
parataxis |
Gibco,F12 |
R6481 |
T14509 |
T14508 |
punct |
", ",Gibco |
R6482 |
T14510 |
T14511 |
compound |
San,Diego |
R6483 |
T14511 |
T14508 |
npadvmod |
Diego,Gibco |
R6484 |
T14512 |
T14508 |
punct |
", ",Gibco |
R6485 |
T14513 |
T14508 |
npadvmod |
California,Gibco |
R6486 |
T14514 |
T14508 |
punct |
", ",Gibco |
R6487 |
T14515 |
T14516 |
compound |
United,States |
R6488 |
T14516 |
T14508 |
npadvmod |
States,Gibco |
R6489 |
T14517 |
T14508 |
punct |
),Gibco |
R6490 |
T14518 |
T14502 |
punct |
", ",coverslips |
R6491 |
T14519 |
T14520 |
nummod |
2,mM |
R6492 |
T14520 |
T14521 |
compound |
mM,Gln |
R6493 |
T14521 |
T14502 |
conj |
Gln,coverslips |
R6494 |
T14522 |
T14521 |
compound |
L,Gln |
R6495 |
T14523 |
T14521 |
punct |
-,Gln |
R6496 |
T14524 |
T14525 |
punct |
(,Gibco |
R6497 |
T14525 |
T14521 |
parataxis |
Gibco,Gln |
R6498 |
T14526 |
T14525 |
punct |
),Gibco |
R6499 |
T14527 |
T14521 |
punct |
", ",Gln |
R6500 |
T14528 |
T14521 |
conj |
N2,Gln |
R6501 |
T14529 |
T14530 |
punct |
(,Gibco |
R6502 |
T14530 |
T14528 |
parataxis |
Gibco,N2 |
R6503 |
T14531 |
T14530 |
punct |
),Gibco |
R6504 |
T14532 |
T14528 |
punct |
", ",N2 |
R6505 |
T14533 |
T14528 |
cc |
and,N2 |
R6506 |
T14534 |
T14535 |
nummod |
1,mg |
R6507 |
T14535 |
T14536 |
nmod |
mg,BSA |
R6508 |
T14536 |
T14528 |
conj |
BSA,N2 |
R6509 |
T14537 |
T14538 |
punct |
/,ml |
R6510 |
T14538 |
T14535 |
prep |
ml,mg |
R6511 |
T14539 |
T14540 |
punct |
(,Sigma |
R6512 |
T14540 |
T14536 |
parataxis |
Sigma,BSA |
R6513 |
T14541 |
T14540 |
punct |
", ",Sigma |
R6514 |
T14542 |
T14543 |
compound |
St.,Louis |
R6515 |
T14543 |
T14540 |
npadvmod |
Louis,Sigma |
R6516 |
T14544 |
T14540 |
punct |
", ",Sigma |
R6517 |
T14545 |
T14540 |
npadvmod |
Missouri,Sigma |
R6518 |
T14546 |
T14540 |
punct |
", ",Sigma |
R6519 |
T14547 |
T14548 |
compound |
United,States |
R6520 |
T14548 |
T14540 |
npadvmod |
States,Sigma |
R6521 |
T14549 |
T14540 |
punct |
),Sigma |
R6522 |
T14550 |
T14536 |
prep |
without,BSA |
R6523 |
T14551 |
T14550 |
pobj |
neurotrophins,without |
R6524 |
T14552 |
T14478 |
punct |
", ",dissected |
R6525 |
T14553 |
T14478 |
cc |
or,dissected |
R6526 |
T14554 |
T14478 |
conj |
supplemented,dissected |
R6527 |
T14555 |
T14554 |
prep |
with,supplemented |
R6528 |
T14556 |
T14557 |
preconj |
either,NGF |
R6529 |
T14557 |
T14555 |
pobj |
NGF,with |
R6530 |
T14558 |
T14559 |
punct |
(,Gibco |
R6531 |
T14559 |
T14557 |
parataxis |
Gibco,NGF |
R6532 |
T14560 |
T14561 |
nummod |
100,ng |
R6533 |
T14561 |
T14559 |
dep |
ng,Gibco |
R6534 |
T14562 |
T14563 |
punct |
/,ml |
R6535 |
T14563 |
T14561 |
prep |
ml,ng |
R6536 |
T14564 |
T14559 |
punct |
", ",Gibco |
R6537 |
T14565 |
T14559 |
punct |
),Gibco |
R6538 |
T14566 |
T14557 |
cc |
or,NGF |
R6539 |
T14567 |
T14557 |
conj |
NT,NGF |
R6540 |
T14568 |
T14567 |
punct |
-,NT |
R6541 |
T14569 |
T14567 |
nummod |
3,NT |
R6542 |
T14570 |
T14571 |
punct |
(,Sigma |
R6543 |
T14571 |
T14567 |
parataxis |
Sigma,NT |
R6544 |
T14572 |
T14573 |
nummod |
20,ng |
R6545 |
T14573 |
T14571 |
dep |
ng,Sigma |
R6546 |
T14574 |
T14575 |
punct |
/,ml |
R6547 |
T14575 |
T14573 |
prep |
ml,ng |
R6548 |
T14576 |
T14571 |
punct |
", ",Sigma |
R6549 |
T14577 |
T14571 |
punct |
),Sigma |
R6550 |
T14578 |
T14478 |
punct |
.,dissected |
R6551 |
T14580 |
T14581 |
compound |
DRG,explants |
R6552 |
T14581 |
T14582 |
nsubjpass |
explants,cultured |
R6553 |
T14583 |
T14584 |
punct |
(,20 |
R6554 |
T14584 |
T14581 |
parataxis |
20,explants |
R6555 |
T14585 |
T14584 |
nsubj |
n,20 |
R6556 |
T14586 |
T14584 |
punct |
≥,20 |
R6557 |
T14587 |
T14584 |
prep |
for,20 |
R6558 |
T14588 |
T14589 |
det |
each,condition |
R6559 |
T14589 |
T14587 |
pobj |
condition,for |
R6560 |
T14590 |
T14584 |
punct |
),20 |
R6561 |
T14591 |
T14582 |
auxpass |
were,cultured |
R6562 |
T14592 |
T14582 |
prep |
for,cultured |
R6563 |
T14593 |
T14594 |
nummod |
48,h |
R6564 |
T14594 |
T14592 |
pobj |
h,for |
R6565 |
T14595 |
T14582 |
punct |
", ",cultured |
R6566 |
T14596 |
T14582 |
conj |
processed,cultured |
R6567 |
T14597 |
T14596 |
prep |
for,processed |
R6568 |
T14598 |
T14597 |
pobj |
immunocytochemistry,for |
R6569 |
T14599 |
T14596 |
punct |
", ",processed |
R6570 |
T14600 |
T14596 |
cc |
and,processed |
R6571 |
T14601 |
T14596 |
conj |
analyzed,processed |
R6572 |
T14602 |
T14601 |
advcl |
using,analyzed |
R6573 |
T14603 |
T14604 |
amod |
confocal,microscopy |
R6574 |
T14604 |
T14602 |
dobj |
microscopy,using |
R6575 |
T14605 |
T14582 |
punct |
.,cultured |
R6577 |
T14754 |
T14755 |
compound |
Western,blot |
R6578 |
T14755 |
T14756 |
compound |
blot,analysis |
R6579 |
T14758 |
T14759 |
compound |
Lumbar,DRG |
R6580 |
T14759 |
T14760 |
nsubjpass |
DRG,isolated |
R6581 |
T14761 |
T14759 |
prep |
from,DRG |
R6582 |
T14762 |
T14763 |
compound |
E16.5,embryos |
R6583 |
T14763 |
T14761 |
pobj |
embryos,from |
R6584 |
T14764 |
T14760 |
auxpass |
were,isolated |
R6585 |
T14765 |
T14760 |
punct |
", ",isolated |
R6586 |
T14766 |
T14767 |
advmod |
mechanically,disrupted |
R6587 |
T14767 |
T14760 |
conj |
disrupted,isolated |
R6588 |
T14768 |
T14767 |
punct |
", ",disrupted |
R6589 |
T14769 |
T14767 |
conj |
homogenized,disrupted |
R6590 |
T14770 |
T14769 |
advcl |
using,homogenized |
R6591 |
T14771 |
T14772 |
compound |
glass,beads |
R6592 |
T14772 |
T14770 |
dobj |
beads,using |
R6593 |
T14773 |
T14774 |
punct |
(,Sigma |
R6594 |
T14774 |
T14772 |
parataxis |
Sigma,beads |
R6595 |
T14775 |
T14774 |
punct |
),Sigma |
R6596 |
T14776 |
T14769 |
punct |
", ",homogenized |
R6597 |
T14777 |
T14769 |
cc |
and,homogenized |
R6598 |
T14778 |
T14769 |
conj |
lysed,homogenized |
R6599 |
T14779 |
T14778 |
prep |
in,lysed |
R6600 |
T14780 |
T14781 |
amod |
standard,buffer |
R6601 |
T14781 |
T14779 |
pobj |
buffer,in |
R6602 |
T14782 |
T14781 |
compound |
lysis,buffer |
R6603 |
T14783 |
T14781 |
acl |
supplemented,buffer |
R6604 |
T14784 |
T14783 |
prep |
with,supplemented |
R6605 |
T14785 |
T14786 |
nmod |
protease,inhibitors |
R6606 |
T14786 |
T14784 |
pobj |
inhibitors,with |
R6607 |
T14787 |
T14785 |
cc |
and,protease |
R6608 |
T14788 |
T14785 |
conj |
phosphatase,protease |
R6609 |
T14789 |
T14790 |
mark |
as,described |
R6610 |
T14790 |
T14783 |
advcl |
described,supplemented |
R6611 |
T14791 |
T14792 |
punct |
[,47 |
R6612 |
T14792 |
T14790 |
parataxis |
47,described |
R6613 |
T14793 |
T14792 |
punct |
],47 |
R6614 |
T14794 |
T14760 |
punct |
.,isolated |
R6615 |
T14796 |
T14797 |
compound |
Protein,extracts |
R6616 |
T14797 |
T14798 |
nsubjpass |
extracts,resolved |
R6617 |
T14799 |
T14798 |
auxpass |
were,resolved |
R6618 |
T14800 |
T14798 |
agent |
by,resolved |
R6619 |
T14801 |
T14802 |
compound |
SDS,PAGE |
R6620 |
T14802 |
T14800 |
pobj |
PAGE,by |
R6621 |
T14803 |
T14802 |
punct |
-,PAGE |
R6622 |
T14804 |
T14798 |
punct |
", ",resolved |
R6623 |
T14805 |
T14798 |
cc |
and,resolved |
R6624 |
T14806 |
T14807 |
nsubjpass |
immunoblotting,performed |
R6625 |
T14807 |
T14798 |
conj |
performed,resolved |
R6626 |
T14808 |
T14807 |
auxpass |
was,performed |
R6627 |
T14809 |
T14807 |
advcl |
using,performed |
R6628 |
T14810 |
T14809 |
dobj |
antibodies,using |
R6629 |
T14811 |
T14810 |
prep |
against,antibodies |
R6630 |
T14812 |
T14811 |
pobj |
Akt,against |
R6631 |
T14813 |
T14812 |
punct |
", ",Akt |
R6632 |
T14814 |
T14815 |
compound |
p,Akt |
R6633 |
T14815 |
T14812 |
conj |
Akt,Akt |
R6634 |
T14816 |
T14815 |
punct |
-,Akt |
R6635 |
T14817 |
T14818 |
punct |
(,Ser473 |
R6636 |
T14818 |
T14815 |
parataxis |
Ser473,Akt |
R6637 |
T14819 |
T14818 |
punct |
),Ser473 |
R6638 |
T14820 |
T14815 |
punct |
", ",Akt |
R6639 |
T14821 |
T14815 |
conj |
CREB,Akt |
R6640 |
T14822 |
T14821 |
punct |
", ",CREB |
R6641 |
T14823 |
T14824 |
compound |
p,CREB |
R6642 |
T14824 |
T14821 |
conj |
CREB,CREB |
R6643 |
T14825 |
T14824 |
punct |
-,CREB |
R6644 |
T14826 |
T14827 |
punct |
(,Ser133 |
R6645 |
T14827 |
T14824 |
parataxis |
Ser133,CREB |
R6646 |
T14828 |
T14827 |
punct |
),Ser133 |
R6647 |
T14829 |
T14824 |
punct |
", ",CREB |
R6648 |
T14830 |
T14824 |
conj |
Bax,CREB |
R6649 |
T14831 |
T14830 |
punct |
", ",Bax |
R6650 |
T14832 |
T14833 |
compound |
Bcl,xl |
R6651 |
T14833 |
T14830 |
conj |
xl,Bax |
R6652 |
T14834 |
T14833 |
punct |
-,xl |
R6653 |
T14835 |
T14836 |
punct |
(,Technology |
R6654 |
T14836 |
T14833 |
parataxis |
Technology,xl |
R6655 |
T14837 |
T14836 |
compound |
Cell,Technology |
R6656 |
T14838 |
T14836 |
compound |
Signaling,Technology |
R6657 |
T14839 |
T14836 |
punct |
", ",Technology |
R6658 |
T14840 |
T14836 |
npadvmod |
Beverly,Technology |
R6659 |
T14841 |
T14836 |
punct |
", ",Technology |
R6660 |
T14842 |
T14836 |
npadvmod |
Massachusetts,Technology |
R6661 |
T14843 |
T14836 |
punct |
", ",Technology |
R6662 |
T14844 |
T14845 |
compound |
United,States |
R6663 |
T14845 |
T14836 |
npadvmod |
States,Technology |
R6664 |
T14846 |
T14836 |
punct |
),Technology |
R6665 |
T14847 |
T14833 |
punct |
", ",xl |
R6666 |
T14848 |
T14833 |
cc |
and,xl |
R6667 |
T14849 |
T14833 |
conj |
Bcl2,xl |
R6668 |
T14850 |
T14851 |
punct |
(,Pharmingen |
R6669 |
T14851 |
T14849 |
parataxis |
Pharmingen,Bcl2 |
R6670 |
T14852 |
T14851 |
compound |
BD,Pharmingen |
R6671 |
T14853 |
T14851 |
punct |
", ",Pharmingen |
R6672 |
T14854 |
T14855 |
compound |
San,Diego |
R6673 |
T14855 |
T14851 |
npadvmod |
Diego,Pharmingen |
R6674 |
T14856 |
T14851 |
punct |
", ",Pharmingen |
R6675 |
T14857 |
T14851 |
npadvmod |
California,Pharmingen |
R6676 |
T14858 |
T14851 |
punct |
", ",Pharmingen |
R6677 |
T14859 |
T14860 |
compound |
United,States |
R6678 |
T14860 |
T14851 |
npadvmod |
States,Pharmingen |
R6679 |
T14861 |
T14851 |
punct |
),Pharmingen |
R6680 |
T14862 |
T14807 |
punct |
.,performed |
R6681 |
T14864 |
T14865 |
prep |
For,scanned |
R6682 |
T14866 |
T14864 |
pobj |
quantification,For |
R6683 |
T14867 |
T14865 |
punct |
", ",scanned |
R6684 |
T14868 |
T14865 |
nsubjpass |
films,scanned |
R6685 |
T14869 |
T14870 |
punct |
(,AR |
R6686 |
T14870 |
T14868 |
parataxis |
AR,films |
R6687 |
T14871 |
T14872 |
compound |
X,OMAT |
R6688 |
T14872 |
T14870 |
compound |
OMAT,AR |
R6689 |
T14873 |
T14872 |
punct |
-,OMAT |
R6690 |
T14874 |
T14870 |
punct |
", ",AR |
R6691 |
T14875 |
T14876 |
compound |
Eastman,Kodak |
R6692 |
T14876 |
T14870 |
npadvmod |
Kodak,AR |
R6693 |
T14877 |
T14870 |
punct |
", ",AR |
R6694 |
T14878 |
T14870 |
npadvmod |
Rochester,AR |
R6695 |
T14879 |
T14870 |
punct |
", ",AR |
R6696 |
T14880 |
T14881 |
compound |
New,York |
R6697 |
T14881 |
T14870 |
npadvmod |
York,AR |
R6698 |
T14882 |
T14870 |
punct |
", ",AR |
R6699 |
T14883 |
T14884 |
compound |
United,States |
R6700 |
T14884 |
T14870 |
npadvmod |
States,AR |
R6701 |
T14885 |
T14870 |
punct |
),AR |
R6702 |
T14886 |
T14865 |
auxpass |
were,scanned |
R6703 |
T14887 |
T14865 |
cc |
and,scanned |
R6704 |
T14888 |
T14889 |
nsubjpass |
densitometry,performed |
R6705 |
T14889 |
T14865 |
conj |
performed,scanned |
R6706 |
T14890 |
T14889 |
auxpass |
was,performed |
R6707 |
T14891 |
T14889 |
advcl |
using,performed |
R6708 |
T14892 |
T14891 |
dobj |
IMAGEQUANT,using |
R6709 |
T14893 |
T14892 |
nummod |
5.2,IMAGEQUANT |
R6710 |
T14894 |
T14895 |
punct |
(,Dynamics |
R6711 |
T14895 |
T14892 |
parataxis |
Dynamics,IMAGEQUANT |
R6712 |
T14896 |
T14895 |
compound |
Molecular,Dynamics |
R6713 |
T14897 |
T14895 |
punct |
", ",Dynamics |
R6714 |
T14898 |
T14895 |
npadvmod |
Amersham,Dynamics |
R6715 |
T14899 |
T14895 |
punct |
", ",Dynamics |
R6716 |
T14900 |
T14895 |
npadvmod |
Uppsala,Dynamics |
R6717 |
T14901 |
T14895 |
punct |
", ",Dynamics |
R6718 |
T14902 |
T14895 |
npadvmod |
Sweden,Dynamics |
R6719 |
T14903 |
T14895 |
punct |
),Dynamics |
R6720 |
T14904 |
T14889 |
punct |
.,performed |
R6721 |
T15022 |
T15023 |
amod |
Electrophysiological,analysis |
R6722 |
T15023 |
T15024 |
nsubjpass |
analysis,performed |
R6723 |
T15025 |
T15024 |
auxpass |
was,performed |
R6724 |
T15026 |
T15027 |
mark |
as,described |
R6725 |
T15027 |
T15024 |
advcl |
described,performed |
R6726 |
T15028 |
T15027 |
advmod |
previously,described |
R6727 |
T15029 |
T15030 |
punct |
[,48 |
R6728 |
T15030 |
T15027 |
parataxis |
48,described |
R6729 |
T15031 |
T15030 |
punct |
],48 |
R6730 |
T15032 |
T15024 |
punct |
.,performed |
R6731 |
T15034 |
T15035 |
advmod |
Briefly,made |
R6732 |
T15036 |
T15035 |
punct |
", ",made |
R6733 |
T15037 |
T15038 |
amod |
intracellular,recordings |
R6734 |
T15038 |
T15035 |
nsubjpass |
recordings,made |
R6735 |
T15039 |
T15038 |
prep |
from,recordings |
R6736 |
T15040 |
T15041 |
amod |
identified,neurons |
R6737 |
T15041 |
T15039 |
pobj |
neurons,from |
R6738 |
T15042 |
T15041 |
compound |
quadriceps,neurons |
R6739 |
T15043 |
T15041 |
compound |
motor,neurons |
R6740 |
T15044 |
T15035 |
auxpass |
were,made |
R6741 |
T15045 |
T15035 |
advcl |
using,made |
R6742 |
T15046 |
T15047 |
amod |
sharp,electrodes |
R6743 |
T15047 |
T15045 |
dobj |
electrodes,using |
R6744 |
T15048 |
T15049 |
punct |
(,MΩ |
R6745 |
T15049 |
T15045 |
parataxis |
MΩ,using |
R6746 |
T15050 |
T15051 |
quantmod |
75,120 |
R6747 |
T15051 |
T15049 |
nummod |
120,MΩ |
R6748 |
T15052 |
T15051 |
punct |
–,120 |
R6749 |
T15053 |
T15049 |
punct |
", ",MΩ |
R6750 |
T15054 |
T15055 |
compound |
3M,KCl |
R6751 |
T15055 |
T15049 |
appos |
KCl,MΩ |
R6752 |
T15056 |
T15049 |
punct |
),MΩ |
R6753 |
T15057 |
T15035 |
punct |
.,made |
R6754 |
T15059 |
T15060 |
amod |
Average,responses |
R6755 |
T15060 |
T15061 |
nsubjpass |
responses,acquired |
R6756 |
T15062 |
T15063 |
punct |
(,trials |
R6757 |
T15063 |
T15060 |
parataxis |
trials,responses |
R6758 |
T15064 |
T15065 |
quantmod |
10,20 |
R6759 |
T15065 |
T15063 |
nummod |
20,trials |
R6760 |
T15066 |
T15065 |
punct |
–,20 |
R6761 |
T15067 |
T15063 |
punct |
),trials |
R6762 |
T15068 |
T15060 |
prep |
from,responses |
R6763 |
T15069 |
T15070 |
compound |
suprathreshold,stimulation |
R6764 |
T15070 |
T15068 |
pobj |
stimulation,from |
R6765 |
T15071 |
T15070 |
compound |
nerve,stimulation |
R6766 |
T15072 |
T15073 |
punct |
(,strength |
R6767 |
T15073 |
T15070 |
parataxis |
strength,stimulation |
R6768 |
T15074 |
T15073 |
nummod |
1.5,strength |
R6769 |
T15075 |
T15074 |
quantmod |
times,1.5 |
R6770 |
T15076 |
T15073 |
det |
the,strength |
R6771 |
T15077 |
T15078 |
dep |
that,evokes |
R6772 |
T15078 |
T15073 |
relcl |
evokes,strength |
R6773 |
T15079 |
T15080 |
amod |
maximal,response |
R6774 |
T15080 |
T15078 |
dobj |
response,evokes |
R6775 |
T15081 |
T15080 |
amod |
monosynaptic,response |
R6776 |
T15082 |
T15073 |
punct |
),strength |
R6777 |
T15083 |
T15070 |
prep |
of,stimulation |
R6778 |
T15084 |
T15085 |
det |
the,nerve |
R6779 |
T15085 |
T15083 |
pobj |
nerve,of |
R6780 |
T15086 |
T15085 |
compound |
quadriceps,nerve |
R6781 |
T15087 |
T15061 |
auxpass |
were,acquired |
R6782 |
T15088 |
T15061 |
prep |
with,acquired |
R6783 |
T15089 |
T15090 |
compound |
LTP,software |
R6784 |
T15090 |
T15088 |
pobj |
software,with |
R6785 |
T15091 |
T15092 |
punct |
[,49 |
R6786 |
T15092 |
T15061 |
parataxis |
49,acquired |
R6787 |
T15093 |
T15092 |
punct |
],49 |
R6788 |
T15094 |
T15061 |
punct |
.,acquired |
R6789 |
T15096 |
T15097 |
advmod |
Only,cells |
R6790 |
T15097 |
T15098 |
nsubjpass |
cells,considered |
R6791 |
T15099 |
T15097 |
prep |
with,cells |
R6792 |
T15100 |
T15101 |
amod |
stable,potentials |
R6793 |
T15101 |
T15099 |
pobj |
potentials,with |
R6794 |
T15102 |
T15101 |
compound |
resting,potentials |
R6795 |
T15103 |
T15104 |
advmod |
more,negative |
R6796 |
T15104 |
T15101 |
amod |
negative,potentials |
R6797 |
T15105 |
T15104 |
prep |
than,negative |
R6798 |
T15106 |
T15107 |
punct |
−,50 |
R6799 |
T15107 |
T15108 |
nummod |
50,mV |
R6800 |
T15108 |
T15105 |
pobj |
mV,than |
R6801 |
T15109 |
T15098 |
auxpass |
were,considered |
R6802 |
T15110 |
T15098 |
prep |
for,considered |
R6803 |
T15111 |
T15110 |
pobj |
analysis,for |
R6804 |
T15112 |
T15098 |
punct |
.,considered |
R6805 |
T15114 |
T15115 |
amod |
Monosynaptic,amplitudes |
R6806 |
T15115 |
T15116 |
nsubjpass |
amplitudes,determined |
R6807 |
T15117 |
T15116 |
auxpass |
were,determined |
R6808 |
T15118 |
T15116 |
advmod |
offline,determined |
R6809 |
T15119 |
T15116 |
advcl |
using,determined |
R6810 |
T15120 |
T15121 |
compound |
custom,routines |
R6811 |
T15121 |
T15119 |
dobj |
routines,using |
R6812 |
T15122 |
T15119 |
prep |
in,using |
R6813 |
T15123 |
T15124 |
det |
the,environment |
R6814 |
T15124 |
T15122 |
pobj |
environment,in |
R6815 |
T15125 |
T15124 |
compound |
Matlab,environment |
R6816 |
T15126 |
T15127 |
punct |
(,Mathworks |
R6817 |
T15127 |
T15124 |
parataxis |
Mathworks,environment |
R6818 |
T15128 |
T15127 |
det |
The,Mathworks |
R6819 |
T15129 |
T15127 |
punct |
", ",Mathworks |
R6820 |
T15130 |
T15127 |
npadvmod |
Natick,Mathworks |
R6821 |
T15131 |
T15127 |
punct |
", ",Mathworks |
R6822 |
T15132 |
T15127 |
npadvmod |
Massachusetts,Mathworks |
R6823 |
T15133 |
T15127 |
punct |
", ",Mathworks |
R6824 |
T15134 |
T15135 |
compound |
United,States |
R6825 |
T15135 |
T15127 |
npadvmod |
States,Mathworks |
R6826 |
T15136 |
T15127 |
punct |
),Mathworks |
R6827 |
T15137 |
T15138 |
mark |
as,described |
R6828 |
T15138 |
T15119 |
advcl |
described,using |
R6829 |
T15139 |
T15138 |
advmod |
previously,described |
R6830 |
T15140 |
T15141 |
punct |
[,48 |
R6831 |
T15141 |
T15138 |
parataxis |
48,described |
R6832 |
T15142 |
T15141 |
punct |
],48 |
R6833 |
T15143 |
T15116 |
punct |
.,determined |
R517 |
T1905 |
T1901 |
conj |
pools,neurons |
R3536 |
T7827 |
T7824 |
aux |
have,shown |
R3537 |
T7828 |
T7824 |
auxpass |
been,shown |
R3543 |
T7833 |
T7832 |
prep |
of,regulators |
R3544 |
T7834 |
T7835 |
amod |
neuronal,survival |
R3545 |
T7835 |
T7833 |
pobj |
survival,of |
R5178 |
T11732 |
T11719 |
advmod |
also,shown |
R5180 |
T11733 |
T11719 |
auxpass |
been,shown |
R5182 |
T11734 |
T11735 |
aux |
to,exhibit |