PMC:1064895 / 8352-9418 JSON TXT

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test3

{"project":"test3","denotations":[{"id":"T6538","span":{"begin":1048,"end":1051},"obj":"Protein"},{"id":"T6537","span":{"begin":700,"end":703},"obj":"Protein"},{"id":"T6536","span":{"begin":154,"end":157},"obj":"Protein"},{"id":"T6535","span":{"begin":5,"end":8},"obj":"Protein"},{"id":"T6533","span":{"begin":1048,"end":1051},"obj":"Protein"},{"id":"T6532","span":{"begin":700,"end":703},"obj":"Protein"},{"id":"T6531","span":{"begin":154,"end":157},"obj":"Protein"},{"id":"T6530","span":{"begin":5,"end":8},"obj":"Protein"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

BioNLP16_Messiy

{"project":"BioNLP16_Messiy","denotations":[{"id":"T6962","span":{"begin":1048,"end":1051},"obj":"Protein"},{"id":"T6961","span":{"begin":700,"end":703},"obj":"Protein"},{"id":"T6960","span":{"begin":154,"end":157},"obj":"Protein"},{"id":"T6959","span":{"begin":5,"end":8},"obj":"Protein"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

BioNLP16_DUT

{"project":"BioNLP16_DUT","denotations":[{"id":"T7223","span":{"begin":1048,"end":1051},"obj":"Protein"},{"id":"T7222","span":{"begin":700,"end":703},"obj":"Protein"},{"id":"T7221","span":{"begin":154,"end":157},"obj":"Protein"},{"id":"T7220","span":{"begin":5,"end":8},"obj":"Protein"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

2_test

{"project":"2_test","denotations":[{"id":"15642134-8576572-4202025","span":{"begin":89,"end":91},"obj":"8576572"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

biosemtest

pmc-enju-pas

bionlp-st-ge-2016-test-proteins

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T6934","span":{"begin":1048,"end":1051},"obj":"Protein"},{"id":"T6933","span":{"begin":700,"end":703},"obj":"Protein"},{"id":"T6932","span":{"begin":154,"end":157},"obj":"Protein"},{"id":"T6931","span":{"begin":5,"end":8},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T6957","span":{"begin":1048,"end":1051},"obj":"http://www.uniprot.org/uniprot/P01730"},{"id":"T6956","span":{"begin":700,"end":703},"obj":"http://www.uniprot.org/uniprot/P01730"},{"id":"T6955","span":{"begin":154,"end":157},"obj":"http://www.uniprot.org/uniprot/P01730"},{"id":"T6954","span":{"begin":5,"end":8},"obj":"http://www.uniprot.org/uniprot/P01730"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T6935","span":{"begin":270,"end":280},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T6940","span":{"begin":270,"end":280},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T6939","span":{"begin":270,"end":280},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T6938","span":{"begin":1054,"end":1058},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T6937","span":{"begin":812,"end":816},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

sentences

{"project":"sentences","denotations":[{"id":"T6733","span":{"begin":997,"end":1066},"obj":"Sentence"},{"id":"T6732","span":{"begin":893,"end":996},"obj":"Sentence"},{"id":"T6731","span":{"begin":661,"end":892},"obj":"Sentence"},{"id":"T6730","span":{"begin":561,"end":660},"obj":"Sentence"},{"id":"T6729","span":{"begin":487,"end":560},"obj":"Sentence"},{"id":"T6728","span":{"begin":314,"end":486},"obj":"Sentence"},{"id":"T6727","span":{"begin":149,"end":313},"obj":"Sentence"},{"id":"T6726","span":{"begin":94,"end":148},"obj":"Sentence"},{"id":"T6725","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"T62","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"T63","span":{"begin":94,"end":148},"obj":"Sentence"},{"id":"T64","span":{"begin":149,"end":223},"obj":"Sentence"},{"id":"T65","span":{"begin":224,"end":313},"obj":"Sentence"},{"id":"T66","span":{"begin":314,"end":486},"obj":"Sentence"},{"id":"T67","span":{"begin":487,"end":560},"obj":"Sentence"},{"id":"T68","span":{"begin":561,"end":660},"obj":"Sentence"},{"id":"T69","span":{"begin":661,"end":892},"obj":"Sentence"},{"id":"T70","span":{"begin":893,"end":996},"obj":"Sentence"},{"id":"T71","span":{"begin":997,"end":1066},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

simple1

{"project":"simple1","denotations":[{"id":"T6952","span":{"begin":1048,"end":1051},"obj":"Protein"},{"id":"T6951","span":{"begin":700,"end":703},"obj":"Protein"},{"id":"T6950","span":{"begin":154,"end":157},"obj":"Protein"},{"id":"T6949","span":{"begin":5,"end":8},"obj":"Protein"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

DLUT931

{"project":"DLUT931","denotations":[{"id":"T6971","span":{"begin":700,"end":703},"obj":"Protein"},{"id":"T6970","span":{"begin":154,"end":157},"obj":"Protein"},{"id":"T6969","span":{"begin":5,"end":8},"obj":"Protein"},{"id":"T6972","span":{"begin":1048,"end":1051},"obj":"Protein"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

bionlp-st-ge-2016-test-ihmc

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T7218","span":{"begin":700,"end":786},"obj":"Binding"},{"id":"T7217","span":{"begin":676,"end":699},"obj":"Entity"},{"id":"T7216","span":{"begin":471,"end":475},"obj":"Protein"},{"id":"T7215","span":{"begin":94,"end":98},"obj":"Entity"},{"id":"T7214","span":{"begin":704,"end":717},"obj":"Protein"},{"id":"T7213","span":{"begin":957,"end":970},"obj":"Entity"},{"id":"T7212","span":{"begin":469,"end":485},"obj":"Protein"},{"id":"T7211","span":{"begin":336,"end":376},"obj":"Protein"},{"id":"T7210","span":{"begin":700,"end":717},"obj":"Protein"},{"id":"T7209","span":{"begin":976,"end":980},"obj":"Protein"},{"id":"T7208","span":{"begin":314,"end":319},"obj":"Entity"},{"id":"T7206","span":{"begin":700,"end":717},"obj":"Protein"},{"id":"T7205","span":{"begin":1044,"end":1065},"obj":"Entity"},{"id":"T7204","span":{"begin":1048,"end":1051},"obj":"Protein"},{"id":"T7203","span":{"begin":128,"end":131},"obj":"Protein"},{"id":"T7202","span":{"begin":149,"end":176},"obj":"Protein"},{"id":"T7201","span":{"begin":904,"end":912},"obj":"Entity"},{"id":"T7199","span":{"begin":0,"end":92},"obj":"Protein"},{"id":"T7198","span":{"begin":343,"end":376},"obj":"Protein"},{"id":"T7197","span":{"begin":1021,"end":1039},"obj":"Entity"},{"id":"T7196","span":{"begin":243,"end":280},"obj":"Entity"},{"id":"T7195","span":{"begin":809,"end":816},"obj":"Entity"},{"id":"T7194","span":{"begin":817,"end":831},"obj":"Entity"},{"id":"T7193","span":{"begin":561,"end":575},"obj":"Entity"}],"relations":[{"id":"R3929","pred":"themeOf","subj":"T7206","obj":"T7218"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T6947","span":{"begin":1048,"end":1053},"obj":"Protein"},{"id":"T6946","span":{"begin":704,"end":717},"obj":"Protein"},{"id":"T6945","span":{"begin":700,"end":703},"obj":"Protein"},{"id":"T6944","span":{"begin":471,"end":485},"obj":"Protein"},{"id":"T6943","span":{"begin":154,"end":157},"obj":"Protein"},{"id":"T6942","span":{"begin":5,"end":8},"obj":"Protein"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

testone

{"project":"testone","denotations":[{"id":"T6528","span":{"begin":1048,"end":1051},"obj":"Protein"},{"id":"T6527","span":{"begin":700,"end":703},"obj":"Protein"},{"id":"T6526","span":{"begin":154,"end":157},"obj":"Protein"},{"id":"T6525","span":{"begin":5,"end":8},"obj":"Protein"}],"text":"Anti-CD4 microbeads were used essentially as recommended by the manufacturer (Miltenyi) [19]. PBMC were resuspended in 80 μl of FBS staining buffer. Anti-CD4 microbeads (20 μl) were added and incubated for 15 min at 6–12°C. Saturating amounts of fluorochrome-conjugated antibodies were added for a further 10 min. Cells were diluted in 2.5 ml of FBS staining buffer, pelleted, resuspended in 500 μl and magnetically separated, usually on an AutoMACS magnet fitted with a MACS MS column. Flow-through and two 1 ml washes were collected as the negative fraction. Enriched cells were collected in two 0.5 ml aliquots from the column after removal from the magnet. Alternatively, cells stained with anti-CD4–phycoerythrin were washed, magnetically labeled with anti-phycoerythrin microbeads (20 μl added to 80 μl of cell suspension; 15 min, 6–12°C), and magnetically separated as described above. The purity of cells was assessed by flow cytometric analysis of stained cells on a FACS Vantage sorter. Most (more than 97%) of the isolated cells had the CD4 T cell marker."}

PMC:1064895 / 8352-9418 JSONTXT

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PMC:1064895 / 8352-9418 JSON TXT