PMC:1064895 / 12496-13752
Annnotations
test3
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9217 | 808-813 | Protein | denotes | IκB-α |
| T9216 | 800-803 | Protein | denotes | Akt |
| T9215 | 785-799 | Phosphorylation | denotes | phosphorylated |
| T9214 | 780-783 | Protein | denotes | Akt |
| T9213 | 45-48 | Protein | denotes | Akt |
| T9212 | 30-44 | Phosphorylation | denotes | phosphorylated |
| T9211 | 25-28 | Protein | denotes | Akt |
| T9210 | 808-813 | Protein | denotes | IκB-α |
| T9209 | 800-803 | Protein | denotes | Akt |
| T9208 | 780-783 | Protein | denotes | Akt |
| T9207 | 53-58 | Protein | denotes | IκB-α |
| T9206 | 45-48 | Protein | denotes | Akt |
| T9205 | 25-28 | Protein | denotes | Akt |
| R4826 | T9207 | T9212 | themeOf | IκB-α,phosphorylated |
| R4827 | T9213 | T9212 | themeOf | Akt,phosphorylated |
| R4828 | T9216 | T9215 | themeOf | Akt,phosphorylated |
| R4829 | T9217 | T9215 | themeOf | IκB-α,phosphorylated |
BioNLP16_Messiy
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9669 | 785-799 | Phosphorylation | denotes | phosphorylated |
| T9668 | 30-44 | Phosphorylation | denotes | phosphorylated |
| T9667 | 808-813 | Protein | denotes | IκB-α |
| T9666 | 800-803 | Protein | denotes | Akt |
| T9665 | 780-783 | Protein | denotes | Akt |
| T9664 | 53-58 | Protein | denotes | IκB-α |
| T9663 | 45-48 | Protein | denotes | Akt |
| T9662 | 25-28 | Protein | denotes | Akt |
| R5070 | T9663 | T9668 | themeOf | Akt,phosphorylated |
| R5071 | T9666 | T9669 | themeOf | Akt,phosphorylated |
| R5072 | T9667 | T9669 | themeOf | IκB-α,phosphorylated |
BioNLP16_DUT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9967 | 785-799 | Phosphorylation | denotes | phosphorylated |
| T9966 | 30-44 | Phosphorylation | denotes | phosphorylated |
| T9965 | 808-813 | Protein | denotes | IκB-α |
| T9964 | 800-803 | Protein | denotes | Akt |
| T9963 | 780-783 | Protein | denotes | Akt |
| T9962 | 53-58 | Protein | denotes | IκB-α |
| T9961 | 45-48 | Protein | denotes | Akt |
| T9960 | 25-28 | Protein | denotes | Akt |
| R5319 | T9961 | T9966 | themeOf | Akt,phosphorylated |
| R5320 | T9964 | T9967 | themeOf | Akt,phosphorylated |
| R5321 | T9965 | T9967 | themeOf | IκB-α,phosphorylated |
biosemtest
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9361 | 1121-1128 | UMLS/C1548982 | denotes | hybridi |
| T9360 | 956-963 | UMLS/C1548982 | denotes | our tim |
| T9359 | 1081-1087 | UMLS/C1442450 | denotes | room |
| T9358 | 177-183 | UMLS/C1442450 | denotes | ur, wh |
| T9357 | 191-203 | UMLS/C1881488 | denotes | lysates wer |
| T9356 | 593-600 | UMLS/C1705493 | denotes | n hybri |
| T9355 | 456-463 | UMLS/C2347026 | denotes | les wer |
| T9354 | 1213-1216 | UMLS/C0812225 | denotes | ype |
| T9353 | 1213-1216 | UMLS/C1704742 | denotes | ype |
| T9352 | 687-695 | UMLS/C0041415 | denotes | 20 in Tr |
| T9351 | 329-332 | UMLS/C1552937 | denotes | Pr |
| T9350 | 1141-1146 | UMLS/C0175723 | denotes | e det |
| T9349 | 1031-1041 | UMLS/C0003241 | denotes | s were add |
| T9348 | 767-777 | UMLS/C0003241 | denotes | dies again |
| T9347 | 233-238 | UMLS/C1136359 | denotes | s by |
| T9346 | 456-463 | UMLS/C0870078 | denotes | les wer |
| T9345 | 492-496 | UMLS/C0013862 | denotes | nd t |
| T9344 | 1109-1114 | UMLS/C0231290 | denotes | -T wa |
| T9343 | 950-955 | UMLS/C0231290 | denotes | hing |
| T9342 | 156-161 | UMLS/C0231290 | denotes | r inc |
| T9341 | 1010-1020 | UMLS/C0301869 | denotes | d secondar |
| T9340 | 652-664 | UMLS/C0349375 | denotes | d milk in TB |
| T9339 | 745-752 | UMLS/C1292425 | denotes | s, then |
| T9338 | 501-512 | UMLS/C1547239 | denotes | erred to a |
| T9337 | 233-238 | UMLS/C1948049 | denotes | s by |
| T9336 | 334-356 | UMLS/C0427716 | denotes | ein concentrations in |
| T9335 | 895-898 | UP/P02769 | denotes | T, |
| T9334 | 1203-1212 | UMLS/C1511790 | denotes | kit and |
| T9333 | 1152-1160 | UMLS/C1511790 | denotes | with the |
| T9332 | 233-238 | UMLS/C0007634 | denotes | s by |
| T9331 | 1170-1178 | UMLS/C1999177 | denotes | chemilum |
| T9330 | 601-614 | UMLS/C0020202 | denotes | ization, memb |
| T9329 | 329-332 | UMLS/C0439232 | denotes | Pr |
| T9328 | 469-478 | UMLS/C1881713 | denotes | rated by |
| T9327 | 1047-1052 | UMLS/C1524062 | denotes | allo |
| T9326 | 911-916 | UMLS/C1524062 | denotes | d inc |
| T9325 | 469-478 | UMLS/C0443299 | denotes | rated by |
| T9324 | 895-898 | UMLS/C0005902 | denotes | T, |
| T9323 | 329-332 | UMLS/C0702093 | denotes | Pr |
| T9322 | 874-881 | UMLS/C1948037 | denotes | 1:1000 |
| T9321 | 334-356 | UMLS/C1304757 | denotes | ein concentrations in |
| T9320 | 282-293 | UMLS/C0993864 | denotes | rifuged at |
| T9319 | 456-463 | UMLS/C0370003 | denotes | les wer |
| T9318 | 1152-1160 | UMLS/C0442726 | denotes | with the |
| T9317 | 264-269 | UMLS/C0024348 | denotes | s buf |
| T9316 | 501-512 | UMLS/C0348011 | denotes | erred to a |
| T9315 | 1109-1114 | UMLS/C0687676 | denotes | -T wa |
| T9314 | 950-955 | UMLS/C0687676 | denotes | hing |
| T9313 | 156-161 | UMLS/C0687676 | denotes | r inc |
| T9312 | 601-614 | UMLS/C0028602 | denotes | ization, memb |
| T9311 | 501-512 | UMLS/C1555583 | denotes | erred to a |
| T9310 | 1170-1178 | UMLS/C2349975 | denotes | chemilum |
| T9309 | 759-766 | UMLS/C0205225 | denotes | y antib |
| T9308 | 895-898 | UMLS/C0036774 | denotes | T, |
| T9307 | 209-217 | UMLS/C1521827 | denotes | ared fro |
| T9306 | 0-21 | UMLS/C0949466 | denotes | Western blot analysis |
| T9305 | 137-145 | UMLS/C0251991 | denotes | 94002 (2 |
| T9304 | 114-122 | UMLS/C0392148 | denotes | sence or |
| T9303 | 518-532 | UMLS/C0600190 | denotes | ellulose membr |
| T9302 | 969-974 | UMLS/C1704250 | denotes | h TBS |
| T9301 | 1021-1030 | UMLS/C0175668 | denotes | antibodi |
| T9300 | 895-898 | UMLS/C0487992 | denotes | T, |
| T9299 | 61-65 | UMLS/C1321301 | denotes | MC w |
| T9298 | 674-680 | UMLS/C0006353 | denotes | (0.1% |
| T9297 | 270-276 | UMLS/C0006353 | denotes | er, an |
| T9296 | 895-898 | UMLS/C1511233 | denotes | T, |
| T9295 | 838-848 | UMLS/C0039421 | denotes | gy Inc., B |
| T9294 | 759-766 | UMLS/C0439631 | denotes | y antib |
| T9293 | 1010-1020 | UMLS/C0522529 | denotes | d secondar |
| T9292 | 501-512 | UMLS/C1540850 | denotes | erred to a |
| T9291 | 745-752 | UMLS/C1547059 | denotes | s, then |
| T9290 | 1021-1030 | UMLS/C1522484 | denotes | antibodi |
| T9289 | 233-238 | UMLS/C1704653 | denotes | s by |
| T9288 | 501-512 | UMLS/C0040671 | denotes | erred to a |
| T9287 | 382-392 | UMLS/C0521095 | denotes | rmined wit |
| T9286 | 411-417 | UMLS/C0449851 | denotes | od (Bi |
| T9285 | 1179-1196 | UMLS/C0162524 | denotes | nescence (ECL) de |
| T9284 | 1031-1041 | UMLS/C0021027 | denotes | s were add |
| T9283 | 767-777 | UMLS/C0021027 | denotes | dies again |
| T9282 | 1141-1146 | UMLS/C0439645 | denotes | e det |
| T9281 | 713-719 | UMLS/C0036082 | denotes | ) at r |
| T9280 | 580-586 | UMLS/C0038995 | denotes | ). For |
| T9279 | 162-172 | UMLS/C1980044 | denotes | bation for |
| T9278 | 969-974 | UMLS/C1548318 | denotes | h TBS |
| T9277 | 1021-1030 | UMLS/C0027627 | denotes | antibodi |
| T9276 | 126-133 | UMLS/C0424530 | denotes | ence of |
| T9275 | 868-871 | UMLS/C0041703 | denotes | lut |
| T9274 | 442-445 | UMLS/C0041703 | denotes | . P |
| T9273 | 1068-1076 | UMLS/C1439852 | denotes | for 1 ho |
| T9272 | 921-930 | UMLS/C1439852 | denotes | d overnig |
| T9271 | 71-80 | UMLS/C1439852 | denotes | cubated w |
| T9270 | 1047-1052 | UMLS/C1720086 | denotes | allo |
| T9269 | 911-916 | UMLS/C1720086 | denotes | d inc |
| T9268 | 233-238 | UMLS/C1269647 | denotes | s by |
| T9267 | 185-190 | UMLS/C0444667 | denotes | e cel |
| T9266 | 1179-1196 | UMLS/C1450278 | denotes | nescence (ECL) de |
| T9265 | 1198-1201 | UMLS/C1332684 | denotes | cti |
| T9264 | 969-974 | UMLS/C1547403 | denotes | h TBS |
| T9263 | 1057-1064 | UMLS/C0683607 | denotes | o incub |
| T9262 | 969-974 | UMLS/C1632851 | denotes | h TBS |
| T9261 | 114-122 | UMLS/C0150312 | denotes | sence or |
| T9260 | 1065-1067 | UMLS/C0319022 | denotes | te |
| T9259 | 513-515 | UMLS/C0319022 | denotes | it |
| T9258 | 874-881 | UMLS/C1720119 | denotes | 1:1000 |
| T9257 | 931-940 | UMLS/C0439583 | denotes | t at 4°C. |
| T9256 | 1235-1243 | UMLS/C0034760 | denotes | (Amersha |
| T9255 | 969-974 | UMLS/C0392761 | denotes | h TBS |
| T9254 | 1031-1041 | UMLS/C1621287 | denotes | s were add |
| T9253 | 767-777 | UMLS/C1621287 | denotes | dies again |
| T9252 | 162-172 | UMLS/C1979572 | denotes | bation for |
| T9251 | 806-809 | UP/Q8INB9 | denotes | d I |
| T9250 | 786-789 | UP/Q8INB9 | denotes | hos |
| T9249 | 45-48 | UP/Q8INB9 | denotes | Akt |
| T9248 | 25-28 | UP/Q8INB9 | denotes | Akt |
| T9247 | 778-785 | UMLS/C0521124 | denotes | t Akt, |
| T9246 | 1047-1052 | UMLS/C1883712 | denotes | allo |
| T9245 | 911-916 | UMLS/C1883712 | denotes | d inc |
| T9244 | 456-463 | UMLS/C0441621 | denotes | les wer |
| T9243 | 1198-1201 | UP/P83410 | denotes | cti |
| T9242 | 411-417 | UMLS/C0025664 | denotes | od (Bi |
| T9241 | 411-417 | UMLS/C0025663 | denotes | od (Bi |
| T9240 | 969-974 | UMLS/C0449243 | denotes | h TBS |
| T9239 | 969-974 | UMLS/C0040223 | denotes | h TBS |
| T9238 | 1198-1201 | UP/P55797 | denotes | cti |
| T9237 | 1213-1216 | UMLS/C1705212 | denotes | ype |
| T9236 | 1141-1146 | UMLS/C0185014 | denotes | e det |
| T9235 | 501-512 | UMLS/C0728827 | denotes | erred to a |
| T9234 | 126-133 | UMLS/C0332197 | denotes | ence of |
| T9233 | 364-376 | UMLS/C1550101 | denotes | rnatants wer |
| T9232 | 1213-1216 | UMLS/C1690540 | denotes | ype |
| T9231 | 964-968 | UMLS/C0205450 | denotes | s wi |
| T9230 | 823-837 | UMLS/C0037083 | denotes | naling Technol |
| T9229 | 126-133 | UMLS/C1689985 | denotes | ence of |
| T9228 | 987-1009 | UMLS/C0019941 | denotes | sh peroxidase-conjugat |
| T9227 | 448-455 | UMLS/C0033684 | denotes | ein sam |
| T9226 | 501-512 | UMLS/C1705822 | denotes | erred to a |
| T9225 | 1213-1216 | UMLS/C1553450 | denotes | ype |
| T9224 | 1091-1107 | UMLS/C2348236 | denotes | erature. After T |
| T9223 | 724-740 | UMLS/C2348236 | denotes | emperature for 2 |
| T9222 | 1021-1030 | UMLS/C0205436 | denotes | antibodi |
| T9221 | 469-478 | UMLS/C0086972 | denotes | rated by |
| T9220 | 501-512 | UMLS/C1299988 | denotes | erred to a |
| T9219 | 264-269 | UMLS/C1536403 | denotes | s buf |
pmc-enju-pas
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9604 | 1254-1255 | -RRB- | denotes | ) |
| T9603 | 1245-1254 | NNP | denotes | Pharmacia |
| T9602 | 1236-1244 | NNP | denotes | Amersham |
| T9601 | 1235-1236 | -LRB- | denotes | ( |
| T9600 | 1226-1234 | NN | denotes | reagents |
| T9599 | 1212-1225 | NN | denotes | Hyperfilm-ECL |
| T9598 | 1208-1211 | CC | denotes | and |
| T9597 | 1204-1207 | NN | denotes | kit |
| T9596 | 1194-1203 | NN | denotes | detection |
| T9595 | 1192-1193 | -RRB- | denotes | ) |
| T9594 | 1189-1192 | NN | denotes | ECL |
| T9593 | 1188-1189 | -LRB- | denotes | ( |
| T9592 | 1170-1187 | NN | denotes | chemiluminescence |
| T9591 | 1161-1169 | VB | denotes | enhanced |
| T9590 | 1157-1160 | DT | denotes | the |
| T9589 | 1152-1156 | IN | denotes | with |
| T9588 | 1143-1151 | VB | denotes | detected |
| T9587 | 1138-1142 | VB | denotes | were |
| T9586 | 1132-1137 | NN | denotes | bands |
| T9585 | 1121-1131 | VB | denotes | hybridized |
| T9584 | 1119-1120 | -COMMA- | denotes | , |
| T9583 | 1112-1119 | NN | denotes | washing |
| T9582 | 1106-1111 | NN | denotes | TBS-T |
| T9581 | 1100-1105 | IN | denotes | After |
| T9580 | 1087-1098 | NN | denotes | temperature |
| T9579 | 1082-1086 | NN | denotes | room |
| T9578 | 1079-1081 | IN | denotes | at |
| T9577 | 1074-1078 | NN | denotes | hour |
| T9576 | 1072-1073 | CD | denotes | 1 |
| T9575 | 1068-1071 | IN | denotes | for |
| T9574 | 1059-1067 | VB | denotes | incubate |
| T9573 | 1056-1058 | TO | denotes | to |
| T9572 | 1048-1055 | VB | denotes | allowed |
| T9571 | 1044-1047 | CC | denotes | and |
| T9570 | 1038-1043 | VB | denotes | added |
| T9569 | 1033-1037 | VB | denotes | were |
| T9568 | 1022-1032 | NN | denotes | antibodies |
| T9567 | 1012-1021 | JJ | denotes | secondary |
| T9566 | 990-1011 | JJ | denotes | peroxidase-conjugated |
| T9565 | 978-989 | JJ | denotes | horseradish |
| T9564 | 976-977 | -COMMA- | denotes | , |
| T9563 | 971-976 | NN | denotes | TBS-T |
| T9562 | 966-970 | IN | denotes | with |
| T9561 | 960-965 | NN | denotes | times |
| T9560 | 955-959 | CD | denotes | four |
| T9559 | 947-954 | VB | denotes | washing |
| T9558 | 941-946 | IN | denotes | After |
| T9557 | 936-939 | NN | denotes | 4°C |
| T9556 | 933-935 | IN | denotes | at |
| T9555 | 923-932 | RB | denotes | overnight |
| T9554 | 913-922 | VB | denotes | incubated |
| T9553 | 909-912 | CC | denotes | and |
| T9552 | 903-908 | VB | denotes | added |
| T9551 | 898-902 | VB | denotes | were |
| T9550 | 896-897 | -COMMA- | denotes | , |
| T9549 | 887-896 | NN | denotes | BSA/TBS-T |
| T9548 | 885-886 | NN | denotes | % |
| T9547 | 884-885 | CD | denotes | 5 |
| T9546 | 881-883 | IN | denotes | in |
| T9545 | 876-880 | CD | denotes | 1000 |
| T9544 | 875-876 | -COLON- | denotes | : |
| T9543 | 874-875 | CD | denotes | 1 |
| T9542 | 866-873 | VB | denotes | diluted |
| T9541 | 864-865 | -COMMA- | denotes | , |
| T9540 | 863-864 | -RRB- | denotes | ) |
| T9539 | 860-863 | NNP | denotes | USA |
| T9538 | 858-859 | -COMMA- | denotes | , |
| T9537 | 856-858 | NNP | denotes | MA |
| T9536 | 854-855 | -COMMA- | denotes | , |
| T9535 | 847-854 | NNP | denotes | Beverly |
| T9534 | 845-846 | -COMMA- | denotes | , |
| T9533 | 841-845 | NNP | denotes | Inc. |
| T9532 | 830-840 | NNP | denotes | Technology |
| T9531 | 820-829 | NNP | denotes | Signaling |
| T9530 | 815-819 | NNP | denotes | Cell |
| T9529 | 814-815 | -LRB- | denotes | ( |
| T9528 | 808-813 | NN | denotes | IκB-α |
| T9527 | 804-807 | CC | denotes | and |
| T9526 | 800-803 | NN | denotes | Akt |
| T9525 | 785-799 | VB | denotes | phosphorylated |
| T9524 | 783-784 | -COMMA- | denotes | , |
| T9523 | 780-783 | NNP | denotes | Akt |
| T9522 | 772-779 | IN | denotes | against |
| T9521 | 761-771 | NN | denotes | antibodies |
| T9520 | 753-760 | JJ | denotes | primary |
| T9519 | 748-752 | RB | denotes | then |
| T9518 | 746-747 | -COMMA- | denotes | , |
| T9517 | 741-746 | NN | denotes | hours |
| T9516 | 739-740 | CD | denotes | 2 |
| T9515 | 735-738 | IN | denotes | for |
| T9514 | 723-734 | NN | denotes | temperature |
| T9513 | 718-722 | NN | denotes | room |
| T9512 | 715-717 | IN | denotes | at |
| T9511 | 713-714 | -RRB- | denotes | ) |
| T9510 | 707-713 | NN | denotes | saline |
| T9509 | 693-706 | JJ | denotes | Tris-buffered |
| T9508 | 690-692 | IN | denotes | in |
| T9507 | 687-689 | CD | denotes | 20 |
| T9506 | 681-686 | CD | denotes | Tween |
| T9505 | 679-680 | NN | denotes | % |
| T9504 | 676-679 | CD | denotes | 0.1 |
| T9503 | 675-676 | -LRB- | denotes | ( |
| T9502 | 668-674 | NN | denotes | buffer |
| T9501 | 662-667 | NN | denotes | TBS-T |
| T9500 | 659-661 | IN | denotes | in |
| T9499 | 654-658 | NN | denotes | milk |
| T9498 | 646-653 | VB | denotes | skimmed |
| T9497 | 644-645 | NN | denotes | % |
| T9496 | 641-644 | CD | denotes | 0.1 |
| T9495 | 636-640 | IN | denotes | with |
| T9494 | 623-635 | VB | denotes | preincubated |
| T9493 | 619-622 | VB | denotes | was |
| T9492 | 610-618 | NN | denotes | membrane |
| T9491 | 608-609 | -COMMA- | denotes | , |
| T9490 | 595-608 | NN | denotes | hybridization |
| T9489 | 587-594 | JJ | denotes | western |
| T9488 | 583-586 | IN | denotes | For |
| T9487 | 580-581 | -RRB- | denotes | ) |
| T9486 | 574-580 | NNP | denotes | Sweden |
| T9485 | 572-573 | -COMMA- | denotes | , |
| T9484 | 565-572 | NNP | denotes | Uppsala |
| T9483 | 563-564 | -COMMA- | denotes | , |
| T9482 | 556-563 | NNP | denotes | Biotech |
| T9481 | 546-555 | NNP | denotes | Pharmacia |
| T9480 | 537-545 | NNP | denotes | Amersham |
| T9479 | 536-537 | -LRB- | denotes | ( |
| T9478 | 527-535 | NN | denotes | membrane |
| T9477 | 512-526 | NN | denotes | nitrocellulose |
| T9476 | 510-511 | DT | denotes | a |
| T9475 | 507-509 | TO | denotes | to |
| T9474 | 495-506 | VB | denotes | transferred |
| T9473 | 491-494 | CC | denotes | and |
| T9472 | 482-490 | NN | denotes | SDS–PAGE |
| T9471 | 480-481 | NN | denotes | % |
| T9470 | 478-480 | CD | denotes | 10 |
| T9469 | 475-477 | IN | denotes | by |
| T9468 | 465-474 | VB | denotes | separated |
| T9467 | 460-464 | VB | denotes | were |
| T9466 | 452-459 | NN | denotes | samples |
| T9465 | 444-451 | NN | denotes | Protein |
| T9464 | 441-442 | -RRB- | denotes | ) |
| T9463 | 438-441 | NNP | denotes | USA |
| T9462 | 436-437 | -COMMA- | denotes | , |
| T9461 | 434-436 | NNP | denotes | CA |
| T9460 | 432-433 | -COMMA- | denotes | , |
| T9459 | 424-432 | NNP | denotes | Hercules |
| T9458 | 422-423 | -COMMA- | denotes | , |
| T9457 | 415-422 | NNP | denotes | Bio-Rad |
| T9456 | 414-415 | -LRB- | denotes | ( |
| T9455 | 407-413 | NN | denotes | method |
| T9454 | 398-406 | NNP | denotes | Bradford |
| T9453 | 394-397 | DT | denotes | the |
| T9452 | 389-393 | IN | denotes | with |
| T9451 | 378-388 | VB | denotes | determined |
| T9450 | 373-377 | VB | denotes | were |
| T9449 | 360-372 | NN | denotes | supernatants |
| T9448 | 356-359 | DT | denotes | the |
| T9447 | 353-355 | IN | denotes | in |
| T9446 | 338-352 | NN | denotes | concentrations |
| T9445 | 330-337 | NN | denotes | Protein |
| T9444 | 325-328 | NN | denotes | min |
| T9443 | 322-324 | CD | denotes | 15 |
| T9442 | 318-321 | IN | denotes | for |
| T9441 | 316-317 | -RRB- | denotes | ) |
| T9440 | 315-316 | NN | denotes | g |
| T9439 | 308-314 | CD | denotes | 19,000 |
| T9438 | 307-308 | -LRB- | denotes | ( |
| T9437 | 300-306 | NN | denotes | r.p.m. |
| T9436 | 293-299 | CD | denotes | 14,000 |
| T9435 | 290-292 | IN | denotes | at |
| T9434 | 278-289 | VB | denotes | centrifuged |
| T9433 | 274-277 | CC | denotes | and |
| T9432 | 272-273 | -COMMA- | denotes | , |
| T9431 | 266-272 | NN | denotes | buffer |
| T9430 | 260-265 | NN | denotes | lysis |
| T9429 | 256-259 | DT | denotes | the |
| T9428 | 253-255 | IN | denotes | in |
| T9427 | 238-252 | NN | denotes | homogenization |
| T9426 | 235-237 | IN | denotes | by |
| T9425 | 229-234 | NN | denotes | cells |
| T9424 | 225-228 | CD | denotes | 107 |
| T9423 | 219-224 | IN | denotes | about |
| T9422 | 214-218 | IN | denotes | from |
| T9421 | 205-213 | VB | denotes | prepared |
| T9420 | 200-204 | VB | denotes | were |
| T9419 | 192-199 | NN | denotes | lysates |
| T9418 | 187-191 | NN | denotes | cell |
| T9417 | 181-186 | JJ | denotes | whole |
| T9403 | 120-122 | CC | denotes | or |
| T9402 | 111-119 | NN | denotes | presence |
| T9401 | 107-110 | DT | denotes | the |
| T9400 | 104-106 | IN | denotes | in |
| T9399 | 102-103 | -RRB- | denotes | ) |
| T9398 | 97-102 | NN | denotes | μg/ml |
| T9397 | 94-96 | CD | denotes | 10 |
| T9396 | 93-94 | -LRB- | denotes | ( |
| T9395 | 84-92 | NN | denotes | anti-CD3 |
| T9394 | 79-83 | IN | denotes | with |
| T9393 | 69-78 | VB | denotes | incubated |
| T9392 | 64-68 | VB | denotes | were |
| T9391 | 59-63 | NN | denotes | PBMC |
| T9390 | 53-58 | NN | denotes | IκB-α |
| T9389 | 49-52 | CC | denotes | and |
| T9388 | 45-48 | NN | denotes | Akt |
| T9387 | 30-44 | VB | denotes | phosphorylated |
| T9386 | 28-29 | -COMMA- | denotes | , |
| T9385 | 25-28 | NN | denotes | Akt |
| T9384 | 22-24 | IN | denotes | of |
| T9383 | 13-21 | NN | denotes | analysis |
| T9382 | 8-12 | NN | denotes | blot |
| T9381 | 0-7 | NN | denotes | Western |
| T9416 | 179-180 | -COMMA- | denotes | , |
| T9415 | 175-179 | NN | denotes | hour |
| T9414 | 173-174 | CD | denotes | 1 |
| T9413 | 169-172 | IN | denotes | for |
| T9412 | 158-168 | NN | denotes | incubation |
| T9411 | 152-157 | IN | denotes | After |
| T9410 | 149-150 | -RRB- | denotes | ) |
| T9409 | 147-149 | NN | denotes | μM |
| T9408 | 144-146 | CD | denotes | 20 |
| T9407 | 143-144 | -LRB- | denotes | ( |
| T9406 | 134-142 | NN | denotes | LY294002 |
| T9405 | 131-133 | IN | denotes | of |
| T9404 | 123-130 | NN | denotes | absence |
| R4834 | T9383 | T9381 | arg1Of | analysis,Western |
| R4835 | T9383 | T9382 | arg1Of | analysis,blot |
| R4836 | T9383 | T9384 | arg1Of | analysis,of |
| R4837 | T9385 | T9386 | arg1Of | Akt,"," |
| R4838 | T9386 | T9389 | arg1Of | ",",and |
| R4839 | T9388 | T9386 | arg2Of | Akt,"," |
| R4840 | T9388 | T9387 | arg2Of | Akt,phosphorylated |
| R4841 | T9389 | T9384 | arg2Of | and,of |
| R4842 | T9390 | T9389 | arg2Of | IκB-α,and |
| R4843 | T9391 | T9392 | arg1Of | PBMC,were |
| R4844 | T9391 | T9393 | arg2Of | PBMC,incubated |
| R4845 | T9393 | T9392 | arg2Of | incubated,were |
| R4846 | T9393 | T9394 | arg1Of | incubated,with |
| R4847 | T9393 | T9400 | arg1Of | incubated,in |
| R4848 | T9395 | T9394 | arg2Of | anti-CD3,with |
| R4849 | T9395 | T9396 | arg1Of | anti-CD3,( |
| R4850 | T9398 | T9396 | arg2Of | μg/ml,( |
| R4851 | T9398 | T9397 | arg1Of | μg/ml,10 |
| R4852 | T9399 | T9396 | arg3Of | ),( |
| R4853 | T9402 | T9403 | arg1Of | presence,or |
| R4854 | T9403 | T9400 | arg2Of | or,in |
| R4855 | T9403 | T9401 | arg1Of | or,the |
| R4856 | T9403 | T9405 | arg1Of | or,of |
| R4857 | T9404 | T9403 | arg2Of | absence,or |
| R4858 | T9406 | T9405 | arg2Of | LY294002,of |
| R4859 | T9406 | T9407 | arg1Of | LY294002,( |
| R4860 | T9409 | T9407 | arg2Of | μM,( |
| R4861 | T9409 | T9408 | arg1Of | μM,20 |
| R4862 | T9410 | T9407 | arg3Of | ),( |
| R4863 | T9412 | T9411 | arg2Of | incubation,After |
| R4864 | T9412 | T9413 | arg1Of | incubation,for |
| R4865 | T9415 | T9413 | arg2Of | hour,for |
| R4866 | T9415 | T9414 | arg1Of | hour,1 |
| R4867 | T9419 | T9417 | arg1Of | lysates,whole |
| R4868 | T9419 | T9418 | arg1Of | lysates,cell |
| R4869 | T9419 | T9420 | arg1Of | lysates,were |
| R4870 | T9419 | T9421 | arg2Of | lysates,prepared |
| R4871 | T9419 | T9434 | arg2Of | lysates,centrifuged |
| R4872 | T9421 | T9422 | arg1Of | prepared,from |
| R4873 | T9421 | T9433 | arg1Of | prepared,and |
| R4874 | T9424 | T9423 | arg1Of | 107,about |
| R4875 | T9425 | T9422 | arg2Of | cells,from |
| R4876 | T9425 | T9424 | arg1Of | cells,107 |
| R4877 | T9427 | T9421 | arg1Of | homogenization,prepared |
| R4878 | T9427 | T9426 | arg2Of | homogenization,by |
| R4879 | T9427 | T9428 | arg1Of | homogenization,in |
| R4880 | T9431 | T9428 | arg2Of | buffer,in |
| R4881 | T9431 | T9429 | arg1Of | buffer,the |
| R4882 | T9431 | T9430 | arg1Of | buffer,lysis |
| R4883 | T9433 | T9411 | arg1Of | and,After |
| R4884 | T9433 | T9416 | arg1Of | and,"," |
| R4885 | T9433 | T9420 | arg2Of | and,were |
| R4886 | T9433 | T9432 | arg1Of | and,"," |
| R4887 | T9434 | T9433 | arg2Of | centrifuged,and |
| R4888 | T9434 | T9435 | arg1Of | centrifuged,at |
| R4889 | T9434 | T9442 | arg1Of | centrifuged,for |
| R4890 | T9437 | T9435 | arg2Of | r.p.m.,at |
| R4891 | T9437 | T9436 | arg1Of | r.p.m.,"14,000" |
| R4892 | T9437 | T9438 | arg1Of | r.p.m.,( |
| R4893 | T9440 | T9438 | arg2Of | g,( |
| R4894 | T9440 | T9439 | arg1Of | g,"19,000" |
| R4895 | T9441 | T9438 | arg3Of | ),( |
| R4896 | T9444 | T9442 | arg2Of | min,for |
| R4897 | T9444 | T9443 | arg1Of | min,15 |
| R4898 | T9446 | T9445 | arg1Of | concentrations,Protein |
| R4899 | T9446 | T9447 | arg1Of | concentrations,in |
| R4900 | T9446 | T9450 | arg1Of | concentrations,were |
| R4901 | T9446 | T9451 | arg2Of | concentrations,determined |
| R4902 | T9449 | T9447 | arg2Of | supernatants,in |
| R4903 | T9449 | T9448 | arg1Of | supernatants,the |
| R4904 | T9451 | T9450 | arg2Of | determined,were |
| R4905 | T9451 | T9452 | arg1Of | determined,with |
| R4906 | T9451 | T9456 | arg1Of | determined,( |
| R4907 | T9455 | T9452 | arg2Of | method,with |
| R4908 | T9455 | T9453 | arg1Of | method,the |
| R4909 | T9455 | T9454 | arg1Of | method,Bradford |
| R4910 | T9457 | T9458 | arg1Of | Bio-Rad,"," |
| R4911 | T9458 | T9460 | arg1Of | ",","," |
| R4912 | T9459 | T9458 | arg2Of | Hercules,"," |
| R4913 | T9460 | T9456 | arg2Of | ",",( |
| R4914 | T9460 | T9462 | arg1Of | ",","," |
| R4915 | T9460 | T9463 | arg1Of | ",",USA |
| R4916 | T9461 | T9460 | arg2Of | CA,"," |
| R4917 | T9464 | T9456 | arg3Of | ),( |
| R4918 | T9466 | T9465 | arg1Of | samples,Protein |
| R4919 | T9466 | T9467 | arg1Of | samples,were |
| R4920 | T9466 | T9468 | arg2Of | samples,separated |
| R4921 | T9466 | T9474 | arg2Of | samples,transferred |
| R4922 | T9468 | T9473 | arg1Of | separated,and |
| R4923 | T9470 | T9471 | arg1Of | 10,% |
| R4924 | T9472 | T9468 | arg1Of | SDS–PAGE,separated |
| R4925 | T9472 | T9469 | arg2Of | SDS–PAGE,by |
| R4926 | T9472 | T9470 | arg1Of | SDS–PAGE,10 |
| R4927 | T9473 | T9467 | arg2Of | and,were |
| R4928 | T9473 | T9479 | arg1Of | and,( |
| R4929 | T9474 | T9473 | arg2Of | transferred,and |
| R4930 | T9474 | T9475 | arg1Of | transferred,to |
| R4931 | T9478 | T9475 | arg2Of | membrane,to |
| R4932 | T9478 | T9476 | arg1Of | membrane,a |
| R4933 | T9478 | T9477 | arg1Of | membrane,nitrocellulose |
| R4934 | T9482 | T9479 | arg2Of | Biotech,( |
| R4935 | T9482 | T9480 | arg1Of | Biotech,Amersham |
| R4936 | T9482 | T9481 | arg1Of | Biotech,Pharmacia |
| R4937 | T9482 | T9483 | arg1Of | Biotech,"," |
| R4938 | T9484 | T9483 | arg2Of | Uppsala,"," |
| R4939 | T9484 | T9485 | arg1Of | Uppsala,"," |
| R4940 | T9486 | T9485 | arg2Of | Sweden,"," |
| R4941 | T9487 | T9479 | arg3Of | ),( |
| R4942 | T9490 | T9488 | arg2Of | hybridization,For |
| R4943 | T9490 | T9489 | arg1Of | hybridization,western |
| R4944 | T9492 | T9493 | arg1Of | membrane,was |
| R4945 | T9492 | T9494 | arg2Of | membrane,preincubated |
| R4946 | T9494 | T9493 | arg2Of | preincubated,was |
| R4947 | T9494 | T9495 | arg1Of | preincubated,with |
| R4948 | T9494 | T9515 | arg1Of | preincubated,for |
| R4949 | T9494 | T9524 | arg1Of | preincubated,"," |
| R4950 | T9497 | T9496 | arg1Of | %,0.1 |
| R4951 | T9499 | T9495 | arg2Of | milk,with |
| R4952 | T9499 | T9497 | arg1Of | milk,% |
| R4953 | T9499 | T9498 | arg2Of | milk,skimmed |
| R4954 | T9499 | T9500 | arg1Of | milk,in |
| R4955 | T9502 | T9500 | arg2Of | buffer,in |
| R4956 | T9502 | T9501 | arg1Of | buffer,TBS-T |
| R4957 | T9502 | T9503 | arg1Of | buffer,( |
| R4958 | T9502 | T9512 | arg1Of | buffer,at |
| R4959 | T9505 | T9504 | arg1Of | %,0.1 |
| R4960 | T9507 | T9503 | arg2Of | 20,( |
| R4961 | T9507 | T9505 | arg1Of | 20,% |
| R4962 | T9507 | T9506 | arg1Of | 20,Tween |
| R4963 | T9507 | T9508 | arg1Of | 20,in |
| R4964 | T9510 | T9508 | arg2Of | saline,in |
| R4965 | T9510 | T9509 | arg1Of | saline,Tris-buffered |
| R4966 | T9511 | T9503 | arg3Of | ),( |
| R4967 | T9514 | T9512 | arg2Of | temperature,at |
| R4968 | T9514 | T9513 | arg1Of | temperature,room |
| R4969 | T9517 | T9515 | arg2Of | hours,for |
| R4970 | T9517 | T9516 | arg1Of | hours,2 |
| R4971 | T9517 | T9518 | arg1Of | hours,"," |
| R4972 | T9521 | T9518 | arg2Of | antibodies,"," |
| R4973 | T9521 | T9519 | arg1Of | antibodies,then |
| R4974 | T9521 | T9520 | arg1Of | antibodies,primary |
| R4975 | T9521 | T9522 | arg1Of | antibodies,against |
| R4976 | T9523 | T9522 | arg2Of | Akt,against |
| R4977 | T9524 | T9488 | arg1Of | ",",For |
| R4978 | T9524 | T9491 | arg1Of | ",","," |
| R4979 | T9524 | T9544 | arg1Of | ",",: |
| R4980 | T9526 | T9527 | arg1Of | Akt,and |
| R4981 | T9527 | T9525 | arg2Of | and,phosphorylated |
| R4982 | T9527 | T9529 | arg1Of | and,( |
| R4983 | T9527 | T9542 | arg1Of | and,diluted |
| R4984 | T9528 | T9527 | arg2Of | IκB-α,and |
| R4985 | T9533 | T9529 | arg2Of | Inc.,( |
| R4986 | T9533 | T9530 | arg1Of | Inc.,Cell |
| R4987 | T9533 | T9531 | arg1Of | Inc.,Signaling |
| R4988 | T9533 | T9532 | arg1Of | Inc.,Technology |
| R4989 | T9533 | T9534 | arg1Of | Inc.,"," |
| R4990 | T9533 | T9538 | arg1Of | Inc.,"," |
| R4991 | T9533 | T9539 | arg1Of | Inc.,USA |
| R4992 | T9535 | T9536 | arg1Of | Beverly,"," |
| R4993 | T9536 | T9534 | arg2Of | ",","," |
| R4994 | T9537 | T9536 | arg2Of | MA,"," |
| R4995 | T9540 | T9529 | arg3Of | ),( |
| R4996 | T9542 | T9524 | arg2Of | diluted,"," |
| R4997 | T9542 | T9541 | arg1Of | diluted,"," |
| R4998 | T9543 | T9542 | arg2Of | 1,diluted |
| R4999 | T9545 | T9546 | arg1Of | 1000,in |
| R5000 | T9545 | T9551 | arg1Of | 1000,were |
| R5001 | T9545 | T9552 | arg2Of | 1000,added |
| R5002 | T9545 | T9554 | arg2Of | 1000,incubated |
| R5003 | T9547 | T9548 | arg1Of | 5,% |
| R5004 | T9549 | T9546 | arg2Of | BSA/TBS-T,in |
| R5005 | T9549 | T9547 | arg1Of | BSA/TBS-T,5 |
| R5006 | T9552 | T9553 | arg1Of | added,and |
| R5007 | T9553 | T9544 | arg2Of | and,: |
| R5008 | T9553 | T9550 | arg1Of | and,"," |
| R5009 | T9553 | T9551 | arg2Of | and,were |
| R5010 | T9553 | T9556 | arg1Of | and,at |
| R5011 | T9554 | T9553 | arg2Of | incubated,and |
| R5012 | T9554 | T9555 | arg1Of | incubated,overnight |
| R5013 | T9557 | T9556 | arg2Of | 4°C,at |
| R5014 | T9559 | T9558 | arg2Of | washing,After |
| R5015 | T9559 | T9562 | arg1Of | washing,with |
| R5016 | T9561 | T9559 | arg2Of | times,washing |
| R5017 | T9561 | T9560 | arg1Of | times,four |
| R5018 | T9563 | T9562 | arg2Of | TBS-T,with |
| R5019 | T9568 | T9559 | arg1Of | antibodies,washing |
| R5020 | T9568 | T9565 | arg1Of | antibodies,horseradish |
| R5021 | T9568 | T9566 | arg1Of | antibodies,peroxidase-conjugated |
| R5022 | T9568 | T9567 | arg1Of | antibodies,secondary |
| R5023 | T9568 | T9569 | arg1Of | antibodies,were |
| R5024 | T9568 | T9570 | arg2Of | antibodies,added |
| R5025 | T9568 | T9572 | arg2Of | antibodies,allowed |
| R5026 | T9568 | T9574 | arg1Of | antibodies,incubate |
| R5027 | T9570 | T9571 | arg1Of | added,and |
| R5028 | T9571 | T9558 | arg1Of | and,After |
| R5029 | T9571 | T9564 | arg1Of | and,"," |
| R5030 | T9571 | T9569 | arg2Of | and,were |
| R5031 | T9572 | T9571 | arg2Of | allowed,and |
| R5032 | T9574 | T9572 | arg3Of | incubate,allowed |
| R5033 | T9574 | T9573 | arg1Of | incubate,to |
| R5034 | T9574 | T9575 | arg1Of | incubate,for |
| R5035 | T9574 | T9578 | arg1Of | incubate,at |
| R5036 | T9577 | T9575 | arg2Of | hour,for |
| R5037 | T9577 | T9576 | arg1Of | hour,1 |
| R5038 | T9580 | T9578 | arg2Of | temperature,at |
| R5039 | T9580 | T9579 | arg1Of | temperature,room |
| R5040 | T9583 | T9581 | arg2Of | washing,After |
| R5041 | T9583 | T9582 | arg1Of | washing,TBS-T |
| R5042 | T9586 | T9585 | arg2Of | bands,hybridized |
| R5043 | T9586 | T9587 | arg1Of | bands,were |
| R5044 | T9586 | T9588 | arg2Of | bands,detected |
| R5045 | T9588 | T9581 | arg1Of | detected,After |
| R5046 | T9588 | T9584 | arg1Of | detected,"," |
| R5047 | T9588 | T9587 | arg2Of | detected,were |
| R5048 | T9588 | T9589 | arg1Of | detected,with |
| R5049 | T9594 | T9593 | arg2Of | ECL,( |
| R5050 | T9595 | T9593 | arg3Of | ),( |
| R5051 | T9597 | T9592 | arg1Of | kit,chemiluminescence |
| R5052 | T9597 | T9593 | arg1Of | kit,( |
| R5053 | T9597 | T9596 | arg1Of | kit,detection |
| R5054 | T9597 | T9598 | arg1Of | kit,and |
| R5055 | T9598 | T9589 | arg2Of | and,with |
| R5056 | T9598 | T9590 | arg1Of | and,the |
| R5057 | T9598 | T9591 | arg2Of | and,enhanced |
| R5058 | T9600 | T9598 | arg2Of | reagents,and |
| R5059 | T9600 | T9599 | arg1Of | reagents,Hyperfilm-ECL |
| R5060 | T9600 | T9601 | arg1Of | reagents,( |
| R5061 | T9603 | T9601 | arg2Of | Pharmacia,( |
| R5062 | T9603 | T9602 | arg1Of | Pharmacia,Amersham |
| R5063 | T9604 | T9601 | arg3Of | ),( |
bionlp-st-ge-2016-test-proteins
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9610 | 808-813 | Protein | denotes | IκB-α |
| T9609 | 800-803 | Protein | denotes | Akt |
| T9608 | 780-783 | Protein | denotes | Akt |
| T9607 | 53-58 | Protein | denotes | IκB-α |
| T9606 | 45-48 | Protein | denotes | Akt |
| T9605 | 25-28 | Protein | denotes | Akt |
bionlp-st-ge-2016-uniprot
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9650 | 25-28 | http://www.uniprot.org/uniprot/P31751 | denotes | Akt |
| T9649 | 800-803 | http://www.uniprot.org/uniprot/P31749 | denotes | Akt |
| T9648 | 780-783 | http://www.uniprot.org/uniprot/P31749 | denotes | Akt |
| T9647 | 45-48 | http://www.uniprot.org/uniprot/P31749 | denotes | Akt |
| T9646 | 25-28 | http://www.uniprot.org/uniprot/P31749 | denotes | Akt |
| T9661 | 89-92 | http://www.uniprot.org/uniprot/P09693 | denotes | CD3 |
| T9660 | 89-92 | http://www.uniprot.org/uniprot/P07766 | denotes | CD3 |
| T9659 | 89-92 | http://www.uniprot.org/uniprot/P04234 | denotes | CD3 |
| T9658 | 89-92 | http://www.uniprot.org/uniprot/P20963 | denotes | CD3 |
| T9657 | 800-803 | http://www.uniprot.org/uniprot/Q9Y243 | denotes | Akt |
| T9656 | 780-783 | http://www.uniprot.org/uniprot/Q9Y243 | denotes | Akt |
| T9655 | 45-48 | http://www.uniprot.org/uniprot/Q9Y243 | denotes | Akt |
| T9654 | 25-28 | http://www.uniprot.org/uniprot/Q9Y243 | denotes | Akt |
| T9653 | 800-803 | http://www.uniprot.org/uniprot/P31751 | denotes | Akt |
| T9652 | 780-783 | http://www.uniprot.org/uniprot/P31751 | denotes | Akt |
| T9651 | 45-48 | http://www.uniprot.org/uniprot/P31751 | denotes | Akt |
UBERON-AE
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9218 | 654-658 | http://purl.obolibrary.org/obo/UBERON_0001913 | denotes | milk |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9370 | 434-436 | http://purl.obolibrary.org/obo/GO_0033968 | denotes | CA |
| T9369 | 260-265 | http://purl.obolibrary.org/obo/GO_0019835 | denotes | lysis |
GO-MF
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9613 | 1022-1032 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibodies |
| T9612 | 761-771 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibodies |
| T9611 | 434-436 | http://purl.obolibrary.org/obo/GO_0033968 | denotes | CA |
GO-CC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9621 | 1022-1032 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibodies |
| T9620 | 761-771 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibodies |
| T9619 | 1022-1032 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibodies |
| T9618 | 761-771 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibodies |
| T9617 | 610-618 | http://purl.obolibrary.org/obo/GO_0016020 | denotes | membrane |
| T9616 | 527-535 | http://purl.obolibrary.org/obo/GO_0016020 | denotes | membrane |
| T9615 | 229-234 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T9614 | 187-191 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9368 | 1100-1256 | Sentence | denotes | After TBS-T washing, hybridized bands were detected with the enhanced chemiluminescence (ECL) detection kit and Hyperfilm-ECL reagents (Amersham Pharmacia). |
| T9367 | 583-1099 | Sentence | denotes | For western hybridization, membrane was preincubated with 0.1% skimmed milk in TBS-T buffer (0.1% Tween 20 in Tris-buffered saline) at room temperature for 2 hours, then primary antibodies against Akt, phosphorylated Akt and IκB-α (Cell Signaling Technology Inc., Beverly, MA, USA), diluted 1:1000 in 5% BSA/TBS-T, were added and incubated overnight at 4°C. After washing four times with TBS-T, horseradish peroxidase-conjugated secondary antibodies were added and allowed to incubate for 1 hour at room temperature. |
| T9366 | 444-582 | Sentence | denotes | Protein samples were separated by 10% SDS–PAGE and transferred to a nitrocellulose membrane (Amersham Pharmacia Biotech, Uppsala, Sweden). |
| T9365 | 330-443 | Sentence | denotes | Protein concentrations in the supernatants were determined with the Bradford method (Bio-Rad, Hercules, CA, USA). |
| T9364 | 152-329 | Sentence | denotes | After incubation for 1 hour, whole cell lysates were prepared from about 107 cells by homogenization in the lysis buffer, and centrifuged at 14,000 r.p.m. (19,000 g) for 15 min. |
| T9363 | 59-151 | Sentence | denotes | PBMC were incubated with anti-CD3 (10 μg/ml) in the presence or absence of LY294002 (20 μM). |
| T9362 | 0-58 | Sentence | denotes | Western blot analysis of Akt, phosphorylated Akt and IκB-α |
| T92 | 0-58 | Sentence | denotes | Western blot analysis of Akt, phosphorylated Akt and IκB-α |
| T93 | 59-151 | Sentence | denotes | PBMC were incubated with anti-CD3 (10 μg/ml) in the presence or absence of LY294002 (20 μM). |
| T94 | 152-329 | Sentence | denotes | After incubation for 1 hour, whole cell lysates were prepared from about 107 cells by homogenization in the lysis buffer, and centrifuged at 14,000 r.p.m. (19,000 g) for 15 min. |
| T95 | 330-443 | Sentence | denotes | Protein concentrations in the supernatants were determined with the Bradford method (Bio-Rad, Hercules, CA, USA). |
| T96 | 444-582 | Sentence | denotes | Protein samples were separated by 10% SDS–PAGE and transferred to a nitrocellulose membrane (Amersham Pharmacia Biotech, Uppsala, Sweden). |
| T97 | 583-940 | Sentence | denotes | For western hybridization, membrane was preincubated with 0.1% skimmed milk in TBS-T buffer (0.1% Tween 20 in Tris-buffered saline) at room temperature for 2 hours, then primary antibodies against Akt, phosphorylated Akt and IκB-α (Cell Signaling Technology Inc., Beverly, MA, USA), diluted 1:1000 in 5% BSA/TBS-T, were added and incubated overnight at 4°C. |
| T98 | 941-1099 | Sentence | denotes | After washing four times with TBS-T, horseradish peroxidase-conjugated secondary antibodies were added and allowed to incubate for 1 hour at room temperature. |
| T99 | 1100-1256 | Sentence | denotes | After TBS-T washing, hybridized bands were detected with the enhanced chemiluminescence (ECL) detection kit and Hyperfilm-ECL reagents (Amersham Pharmacia). |
simple1
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9645 | 808-813 | Protein | denotes | IκB-α |
| T9644 | 800-803 | Protein | denotes | Akt |
| T9643 | 780-783 | Protein | denotes | Akt |
| T9642 | 53-58 | Protein | denotes | IκB-α |
| T9641 | 45-48 | Protein | denotes | Akt |
| T9640 | 25-28 | Protein | denotes | Akt |
DLUT931
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9677 | 785-799 | Phosphorylation | denotes | phosphorylated |
| T9676 | 30-44 | Phosphorylation | denotes | phosphorylated |
| T9675 | 808-813 | Protein | denotes | IκB-α |
| T9674 | 800-803 | Protein | denotes | Akt |
| T9673 | 780-783 | Protein | denotes | Akt |
| T9672 | 53-58 | Protein | denotes | IκB-α |
| T9671 | 45-48 | Protein | denotes | Akt |
| T9670 | 25-28 | Protein | denotes | Akt |
| R5073 | T9671 | T9676 | themeOf | Akt,phosphorylated |
| R5074 | T9673 | T9677 | themeOf | Akt,phosphorylated |
| R5075 | T9674 | T9677 | themeOf | Akt,phosphorylated |
| R5076 | T9675 | T9677 | themeOf | IκB-α,phosphorylated |
bionlp-st-ge-2016-test-ihmc
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9959 | 0-58 | Phosphorylation | denotes | Western blot analysis of Akt, phosphorylated Akt and IκB-α |
| T9958 | 0-58 | Phosphorylation | denotes | Western blot analysis of Akt, phosphorylated Akt and IκB-α |
| T9957 | 1189-1192 | Protein | denotes | ECL |
| T9956 | 59-63 | Entity | denotes | PBMC |
| T9955 | 53-58 | Protein | denotes | IκB-α |
| T9954 | 610-618 | Entity | denotes | membrane |
| T9953 | 693-706 | Entity | denotes | Tris-buffered |
| T9952 | 1012-1032 | Entity | denotes | secondary antibodies |
| T9951 | 131-150 | Entity | denotes | of LY294002 (20 μM) |
| T9950 | 356-372 | Entity | denotes | the supernatants |
| T9949 | 45-48 | Protein | denotes | Akt |
| T9948 | 1106-1131 | Entity | denotes | TBS-T washing, hybridized |
| T9947 | 84-103 | Protein | denotes | anti-CD3 (10 μg/ml) |
| T9946 | 444-459 | Entity | denotes | Protein samples |
| T9945 | 219-234 | Entity | denotes | about 107 cells |
| T9944 | 693-713 | Entity | denotes | Tris-buffered saline |
| T9943 | 808-813 | Protein | denotes | IκB-α |
| T9942 | 780-783 | Protein | denotes | Akt |
| T9941 | 884-897 | Entity | denotes | 5% BSA/TBS-T, |
| T9940 | 815-819 | Entity | denotes | Cell |
| T9939 | 971-977 | Entity | denotes | TBS-T, |
| T9938 | 662-667 | Entity | denotes | TBS-T |
| T9937 | 748-783 | Entity | denotes | then primary antibodies against Akt |
| T9936 | 22-29 | Protein | denotes | of Akt, |
| T9935 | 800-803 | Protein | denotes | Akt |
| T9934 | 856-865 | Protein | denotes | MA, USA), |
| T9933 | 330-372 | Entity | denotes | Protein concentrations in the supernatants |
| T9932 | 1212-1255 | Entity | denotes | Hyperfilm-ECL reagents (Amersham Pharmacia) |
| R5317 | T9949 | T9958 | themeOf | Akt,"Western blot analysis of Akt, phosphorylated Akt and IκB-α" |
| R5318 | T9955 | T9959 | themeOf | IκB-α,"Western blot analysis of Akt, phosphorylated Akt and IκB-α" |
bionlp-st-ge-2016-spacy-parsed
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9906 | 1170-1187 | NN | denotes | chemiluminescence |
| T9907 | 1188-1189 | -LRB- | denotes | ( |
| T9905 | 1161-1169 | JJ | denotes | enhanced |
| T9904 | 1157-1160 | DT | denotes | the |
| T9903 | 1152-1156 | IN | denotes | with |
| T9902 | 1143-1151 | VBN | denotes | detected |
| T9901 | 1138-1142 | VBD | denotes | were |
| T9900 | 1132-1137 | NNS | denotes | bands |
| T9899 | 1121-1131 | VBN | denotes | hybridized |
| T9898 | 1119-1120 | , | denotes | , |
| T9897 | 1112-1119 | VBG | denotes | washing |
| T9896 | 1106-1111 | NNP | denotes | TBS-T |
| T9895 | 1100-1105 | IN | denotes | After |
| T9894 | 1098-1099 | . | denotes | . |
| T9893 | 1087-1098 | NN | denotes | temperature |
| T9892 | 1082-1086 | NN | denotes | room |
| T9891 | 1079-1081 | IN | denotes | at |
| T9890 | 1074-1078 | NN | denotes | hour |
| T9889 | 1072-1073 | CD | denotes | 1 |
| T9888 | 1068-1071 | IN | denotes | for |
| T9887 | 1059-1067 | VB | denotes | incubate |
| T9886 | 1056-1058 | TO | denotes | to |
| T9885 | 1048-1055 | VBN | denotes | allowed |
| T9884 | 1044-1047 | CC | denotes | and |
| T9883 | 1038-1043 | VBN | denotes | added |
| T9882 | 1033-1037 | VBD | denotes | were |
| T9881 | 1022-1032 | NNS | denotes | antibodies |
| T9880 | 1012-1021 | JJ | denotes | secondary |
| T9879 | 990-1011 | JJ | denotes | peroxidase-conjugated |
| T9878 | 978-989 | JJ | denotes | horseradish |
| T9877 | 976-977 | , | denotes | , |
| T9876 | 971-976 | NNP | denotes | TBS-T |
| T9875 | 966-970 | IN | denotes | with |
| T9874 | 960-965 | NNS | denotes | times |
| T9873 | 955-959 | CD | denotes | four |
| T9872 | 947-954 | VBG | denotes | washing |
| T9871 | 941-946 | IN | denotes | After |
| T9870 | 939-940 | . | denotes | . |
| T9869 | 938-939 | NNP | denotes | C |
| T9868 | 937-938 | CD | denotes | ° |
| T9867 | 936-937 | CD | denotes | 4 |
| T9866 | 933-935 | IN | denotes | at |
| T9865 | 923-932 | JJ | denotes | overnight |
| T9864 | 913-922 | VBN | denotes | incubated |
| T9863 | 909-912 | CC | denotes | and |
| T9862 | 903-908 | VBN | denotes | added |
| T9861 | 898-902 | VBD | denotes | were |
| T9860 | 896-897 | , | denotes | , |
| T9859 | 887-896 | NN | denotes | BSA/TBS-T |
| T9858 | 885-886 | NN | denotes | % |
| T9857 | 884-885 | CD | denotes | 5 |
| T9856 | 881-883 | IN | denotes | in |
| T9855 | 874-880 | CD | denotes | 1:1000 |
| T9854 | 866-873 | VBN | denotes | diluted |
| T9853 | 864-865 | , | denotes | , |
| T9852 | 863-864 | -RRB- | denotes | ) |
| T9851 | 860-863 | NNP | denotes | USA |
| T9850 | 858-859 | , | denotes | , |
| T9849 | 856-858 | NNP | denotes | MA |
| T9848 | 854-855 | , | denotes | , |
| T9847 | 847-854 | NNP | denotes | Beverly |
| T9846 | 845-846 | , | denotes | , |
| T9845 | 841-845 | NNP | denotes | Inc. |
| T9844 | 830-840 | NNP | denotes | Technology |
| T9843 | 820-829 | NNP | denotes | Signaling |
| T9842 | 815-819 | NNP | denotes | Cell |
| T9841 | 814-815 | -LRB- | denotes | ( |
| T9840 | 808-813 | NNP | denotes | IκB-α |
| T9839 | 804-807 | CC | denotes | and |
| T9838 | 800-803 | NNP | denotes | Akt |
| T9837 | 785-799 | VBD | denotes | phosphorylated |
| T9836 | 783-784 | , | denotes | , |
| T9835 | 780-783 | NNP | denotes | Akt |
| T9834 | 772-779 | IN | denotes | against |
| T9833 | 761-771 | NNS | denotes | antibodies |
| T9832 | 753-760 | JJ | denotes | primary |
| T9831 | 748-752 | RB | denotes | then |
| T9830 | 746-747 | , | denotes | , |
| T9829 | 741-746 | NNS | denotes | hours |
| T9828 | 739-740 | CD | denotes | 2 |
| T9827 | 735-738 | IN | denotes | for |
| T9826 | 723-734 | NN | denotes | temperature |
| T9825 | 718-722 | NN | denotes | room |
| T9824 | 715-717 | IN | denotes | at |
| T9823 | 713-714 | -RRB- | denotes | ) |
| T9822 | 707-713 | NN | denotes | saline |
| T9821 | 693-706 | JJ | denotes | Tris-buffered |
| T9820 | 690-692 | IN | denotes | in |
| T9819 | 687-689 | CD | denotes | 20 |
| T9818 | 681-686 | CD | denotes | Tween |
| T9817 | 679-680 | NN | denotes | % |
| T9816 | 676-679 | CD | denotes | 0.1 |
| T9815 | 675-676 | -LRB- | denotes | ( |
| T9814 | 668-674 | NN | denotes | buffer |
| T9813 | 662-667 | NNP | denotes | TBS-T |
| T9812 | 659-661 | IN | denotes | in |
| T9811 | 654-658 | NN | denotes | milk |
| T9810 | 646-653 | VBD | denotes | skimmed |
| T9809 | 644-645 | NN | denotes | % |
| T9808 | 641-644 | CD | denotes | 0.1 |
| T9807 | 636-640 | IN | denotes | with |
| T9806 | 623-635 | VBN | denotes | preincubated |
| T9805 | 619-622 | VBD | denotes | was |
| T9804 | 610-618 | NN | denotes | membrane |
| T9803 | 608-609 | , | denotes | , |
| T9802 | 595-608 | NN | denotes | hybridization |
| T9801 | 587-594 | JJ | denotes | western |
| T9800 | 583-586 | IN | denotes | For |
| T9799 | 581-582 | . | denotes | . |
| T9798 | 580-581 | -RRB- | denotes | ) |
| T9797 | 574-580 | NNP | denotes | Sweden |
| T9796 | 572-573 | , | denotes | , |
| T9795 | 565-572 | NNP | denotes | Uppsala |
| T9794 | 563-564 | , | denotes | , |
| T9793 | 556-563 | NNP | denotes | Biotech |
| T9792 | 546-555 | NNP | denotes | Pharmacia |
| T9791 | 537-545 | NNP | denotes | Amersham |
| T9790 | 536-537 | -LRB- | denotes | ( |
| T9789 | 527-535 | NN | denotes | membrane |
| T9788 | 512-526 | JJ | denotes | nitrocellulose |
| T9787 | 510-511 | DT | denotes | a |
| T9786 | 507-509 | TO | denotes | to |
| T9785 | 495-506 | VBN | denotes | transferred |
| T9784 | 491-494 | CC | denotes | and |
| T9783 | 486-490 | NNP | denotes | PAGE |
| T9782 | 482-485 | NNP | denotes | SDS |
| T9781 | 480-481 | NN | denotes | % |
| T9780 | 478-480 | CD | denotes | 10 |
| T9779 | 475-477 | IN | denotes | by |
| T9778 | 465-474 | VBN | denotes | separated |
| T9777 | 460-464 | VBD | denotes | were |
| T9776 | 452-459 | NNS | denotes | samples |
| T9775 | 444-451 | NN | denotes | Protein |
| T9774 | 442-443 | . | denotes | . |
| T9773 | 441-442 | -RRB- | denotes | ) |
| T9772 | 438-441 | NNP | denotes | USA |
| T9771 | 436-437 | , | denotes | , |
| T9770 | 434-436 | NNP | denotes | CA |
| T9769 | 432-433 | , | denotes | , |
| T9768 | 424-432 | NNP | denotes | Hercules |
| T9767 | 422-423 | , | denotes | , |
| T9766 | 415-422 | NNP | denotes | Bio-Rad |
| T9765 | 414-415 | -LRB- | denotes | ( |
| T9764 | 407-413 | NN | denotes | method |
| T9763 | 398-406 | NNP | denotes | Bradford |
| T9762 | 394-397 | DT | denotes | the |
| T9761 | 389-393 | IN | denotes | with |
| T9760 | 378-388 | VBN | denotes | determined |
| T9759 | 373-377 | VBD | denotes | were |
| T9758 | 360-372 | NNS | denotes | supernatants |
| T9757 | 356-359 | DT | denotes | the |
| T9756 | 353-355 | IN | denotes | in |
| T9755 | 338-352 | NNS | denotes | concentrations |
| T9754 | 330-337 | NN | denotes | Protein |
| T9753 | 328-329 | . | denotes | . |
| T9752 | 325-328 | NN | denotes | min |
| T9751 | 322-324 | CD | denotes | 15 |
| T9750 | 318-321 | IN | denotes | for |
| T9749 | 316-317 | -RRB- | denotes | ) |
| T9748 | 315-316 | NN | denotes | g |
| T9747 | 308-314 | CD | denotes | 19,000 |
| T9746 | 307-308 | -LRB- | denotes | ( |
| T9745 | 300-306 | NN | denotes | r.p.m. |
| T9744 | 293-299 | CD | denotes | 14,000 |
| T9743 | 290-292 | IN | denotes | at |
| T9742 | 278-289 | VBD | denotes | centrifuged |
| T9741 | 274-277 | CC | denotes | and |
| T9740 | 272-273 | , | denotes | , |
| T9739 | 266-272 | NN | denotes | buffer |
| T9738 | 260-265 | NN | denotes | lysis |
| T9737 | 256-259 | DT | denotes | the |
| T9736 | 253-255 | IN | denotes | in |
| T9735 | 238-252 | NN | denotes | homogenization |
| T9734 | 235-237 | IN | denotes | by |
| T9733 | 229-234 | NNS | denotes | cells |
| T9732 | 225-228 | CD | denotes | 107 |
| T9731 | 219-224 | IN | denotes | about |
| T9730 | 214-218 | IN | denotes | from |
| T9729 | 205-213 | VBN | denotes | prepared |
| T9728 | 200-204 | VBD | denotes | were |
| T9727 | 192-199 | NNS | denotes | lysates |
| T9726 | 187-191 | NN | denotes | cell |
| T9725 | 181-186 | JJ | denotes | whole |
| T9724 | 179-180 | , | denotes | , |
| T9723 | 175-179 | NN | denotes | hour |
| T9722 | 173-174 | CD | denotes | 1 |
| T9721 | 169-172 | IN | denotes | for |
| T9720 | 158-168 | NN | denotes | incubation |
| T9719 | 152-157 | IN | denotes | After |
| T9718 | 150-151 | . | denotes | . |
| T9717 | 149-150 | -RRB- | denotes | ) |
| T9716 | 147-149 | NNP | denotes | μM |
| T9715 | 144-146 | CD | denotes | 20 |
| T9714 | 143-144 | -LRB- | denotes | ( |
| T9713 | 134-142 | NNP | denotes | LY294002 |
| T9712 | 131-133 | IN | denotes | of |
| T9711 | 123-130 | NN | denotes | absence |
| T9710 | 120-122 | CC | denotes | or |
| T9709 | 111-119 | NN | denotes | presence |
| T9708 | 107-110 | DT | denotes | the |
| T9707 | 104-106 | IN | denotes | in |
| T9706 | 102-103 | -RRB- | denotes | ) |
| T9705 | 100-102 | NN | denotes | ml |
| T9704 | 99-100 | NN | denotes | / |
| T9703 | 97-99 | NN | denotes | μg |
| T9702 | 94-96 | CD | denotes | 10 |
| T9701 | 93-94 | -LRB- | denotes | ( |
| T9700 | 84-92 | JJ | denotes | anti-CD3 |
| T9699 | 79-83 | IN | denotes | with |
| T9698 | 69-78 | VBN | denotes | incubated |
| T9697 | 64-68 | VBD | denotes | were |
| T9696 | 59-63 | NNP | denotes | PBMC |
| T9695 | 53-58 | NNP | denotes | IκB-α |
| T9694 | 49-52 | CC | denotes | and |
| T9693 | 45-48 | NNP | denotes | Akt |
| T9692 | 30-44 | VBD | denotes | phosphorylated |
| T9919 | 1255-1256 | . | denotes | . |
| T9918 | 1254-1255 | -RRB- | denotes | ) |
| T9917 | 1245-1254 | NNP | denotes | Pharmacia |
| T9916 | 1236-1244 | NNP | denotes | Amersham |
| T9915 | 1235-1236 | -LRB- | denotes | ( |
| T9914 | 1226-1234 | NNS | denotes | reagents |
| T9913 | 1212-1225 | JJ | denotes | Hyperfilm-ECL |
| T9912 | 1208-1211 | CC | denotes | and |
| T9911 | 1204-1207 | NN | denotes | kit |
| T9910 | 1194-1203 | NN | denotes | detection |
| T9909 | 1192-1193 | -RRB- | denotes | ) |
| T9908 | 1189-1192 | NNP | denotes | ECL |
| T9691 | 28-29 | , | denotes | , |
| T9690 | 25-28 | NNP | denotes | Akt |
| T9689 | 22-24 | IN | denotes | of |
| T9688 | 13-21 | NN | denotes | analysis |
| T9687 | 8-12 | NN | denotes | blot |
| T9686 | 0-7 | JJ | denotes | Western |
| R5095 | T9702 | T9703 | nummod | 10,μg |
| R5096 | T9703 | T9705 | nmod | μg,ml |
| R5097 | T9704 | T9705 | compound | /,ml |
| R5098 | T9705 | T9700 | appos | ml,anti-CD3 |
| R5099 | T9706 | T9705 | punct | ),ml |
| R5100 | T9707 | T9698 | prep | in,incubated |
| R5101 | T9708 | T9709 | det | the,presence |
| R5102 | T9709 | T9707 | pobj | presence,in |
| R5103 | T9710 | T9709 | cc | or,presence |
| R5104 | T9711 | T9709 | conj | absence,presence |
| R5105 | T9712 | T9711 | prep | of,absence |
| R5106 | T9713 | T9712 | pobj | LY294002,of |
| R5107 | T9714 | T9716 | punct | (,μM |
| R5108 | T9715 | T9716 | nummod | 20,μM |
| R5109 | T9716 | T9713 | appos | μM,LY294002 |
| R5110 | T9717 | T9716 | punct | ),μM |
| R5111 | T9718 | T9692 | punct | .,phosphorylated |
| R5112 | T9719 | T9729 | prep | After,prepared |
| R5113 | T9720 | T9719 | pobj | incubation,After |
| R5114 | T9721 | T9729 | prep | for,prepared |
| R5115 | T9722 | T9723 | nummod | 1,hour |
| R5116 | T9723 | T9721 | pobj | hour,for |
| R5117 | T9724 | T9729 | punct | ",",prepared |
| R5118 | T9725 | T9727 | amod | whole,lysates |
| R5119 | T9726 | T9727 | compound | cell,lysates |
| R5120 | T9727 | T9729 | nsubjpass | lysates,prepared |
| R5121 | T9728 | T9729 | auxpass | were,prepared |
| R5122 | T9729 | T9729 | ROOT | prepared,prepared |
| R5123 | T9730 | T9729 | prep | from,prepared |
| R5124 | T9731 | T9733 | nmod | about,cells |
| R5125 | T9732 | T9733 | nummod | 107,cells |
| R5126 | T9733 | T9730 | pobj | cells,from |
| R5127 | T9734 | T9733 | prep | by,cells |
| R5128 | T9735 | T9734 | pobj | homogenization,by |
| R5129 | T9736 | T9733 | prep | in,cells |
| R5130 | T9737 | T9739 | det | the,buffer |
| R5131 | T9738 | T9739 | compound | lysis,buffer |
| R5132 | T9739 | T9736 | pobj | buffer,in |
| R5133 | T9740 | T9729 | punct | ",",prepared |
| R5134 | T9741 | T9729 | cc | and,prepared |
| R5135 | T9742 | T9729 | conj | centrifuged,prepared |
| R5136 | T9743 | T9742 | prep | at,centrifuged |
| R5137 | T9744 | T9745 | nummod | "14,000",r.p.m. |
| R5138 | T9745 | T9743 | pobj | r.p.m.,at |
| R5139 | T9746 | T9745 | punct | (,r.p.m. |
| R5140 | T9747 | T9748 | nummod | "19,000",g |
| R5141 | T9748 | T9745 | appos | g,r.p.m. |
| R5142 | T9749 | T9745 | punct | ),r.p.m. |
| R5143 | T9750 | T9742 | prep | for,centrifuged |
| R5144 | T9751 | T9752 | nummod | 15,min |
| R5145 | T9752 | T9750 | pobj | min,for |
| R5146 | T9753 | T9729 | punct | .,prepared |
| R5147 | T9754 | T9755 | compound | Protein,concentrations |
| R5148 | T9755 | T9760 | nsubjpass | concentrations,determined |
| R5149 | T9756 | T9755 | prep | in,concentrations |
| R5150 | T9757 | T9758 | det | the,supernatants |
| R5151 | T9758 | T9756 | pobj | supernatants,in |
| R5152 | T9759 | T9760 | auxpass | were,determined |
| R5153 | T9760 | T9760 | ROOT | determined,determined |
| R5154 | T9761 | T9760 | prep | with,determined |
| R5155 | T9762 | T9764 | det | the,method |
| R5156 | T9763 | T9764 | compound | Bradford,method |
| R5157 | T9764 | T9761 | pobj | method,with |
| R5158 | T9765 | T9766 | punct | (,Bio-Rad |
| R5159 | T9766 | T9764 | appos | Bio-Rad,method |
| R5160 | T9767 | T9766 | punct | ",",Bio-Rad |
| R5161 | T9768 | T9766 | conj | Hercules,Bio-Rad |
| R5162 | T9769 | T9768 | punct | ",",Hercules |
| R5163 | T9770 | T9768 | conj | CA,Hercules |
| R5164 | T9771 | T9770 | punct | ",",CA |
| R5165 | T9772 | T9770 | conj | USA,CA |
| R5166 | T9773 | T9766 | punct | ),Bio-Rad |
| R5167 | T9774 | T9760 | punct | .,determined |
| R5168 | T9775 | T9776 | compound | Protein,samples |
| R5169 | T9776 | T9778 | nsubjpass | samples,separated |
| R5170 | T9777 | T9778 | auxpass | were,separated |
| R5171 | T9778 | T9778 | ROOT | separated,separated |
| R5172 | T9779 | T9778 | prep | by,separated |
| R5173 | T9780 | T9781 | nummod | 10,% |
| R5174 | T9781 | T9779 | pobj | %,by |
| R5175 | T9782 | T9783 | compound | SDS,PAGE |
| R5176 | T9783 | T9778 | npadvmod | PAGE,separated |
| R5177 | T9784 | T9778 | cc | and,separated |
| R5178 | T9785 | T9778 | conj | transferred,separated |
| R5179 | T9786 | T9785 | prep | to,transferred |
| R5180 | T9787 | T9789 | det | a,membrane |
| R5181 | T9788 | T9789 | amod | nitrocellulose,membrane |
| R5182 | T9789 | T9786 | pobj | membrane,to |
| R5183 | T9790 | T9793 | punct | (,Biotech |
| R5184 | T9791 | T9793 | compound | Amersham,Biotech |
| R5185 | T9792 | T9793 | compound | Pharmacia,Biotech |
| R5186 | T9793 | T9789 | appos | Biotech,membrane |
| R5187 | T9794 | T9793 | punct | ",",Biotech |
| R5188 | T9795 | T9793 | conj | Uppsala,Biotech |
| R5189 | T9796 | T9795 | punct | ",",Uppsala |
| R5190 | T9797 | T9795 | conj | Sweden,Uppsala |
| R5191 | T9798 | T9793 | punct | ),Biotech |
| R5192 | T9799 | T9778 | punct | .,separated |
| R5193 | T9800 | T9806 | prep | For,preincubated |
| R5194 | T9801 | T9802 | amod | western,hybridization |
| R5195 | T9802 | T9800 | pobj | hybridization,For |
| R5196 | T9803 | T9806 | punct | ",",preincubated |
| R5197 | T9804 | T9806 | nsubjpass | membrane,preincubated |
| R5198 | T9805 | T9806 | auxpass | was,preincubated |
| R5199 | T9806 | T9806 | ROOT | preincubated,preincubated |
| R5200 | T9807 | T9806 | prep | with,preincubated |
| R5201 | T9808 | T9809 | nummod | 0.1,% |
| R5202 | T9809 | T9810 | nsubj | %,skimmed |
| R5203 | T9810 | T9806 | conj | skimmed,preincubated |
| R5204 | T9811 | T9810 | dobj | milk,skimmed |
| R5205 | T9812 | T9810 | prep | in,skimmed |
| R5206 | T9813 | T9814 | compound | TBS-T,buffer |
| R5207 | T9814 | T9812 | pobj | buffer,in |
| R5208 | T9815 | T9817 | punct | (,% |
| R5209 | T9816 | T9817 | nummod | 0.1,% |
| R5210 | T9817 | T9837 | nsubj | %,phosphorylated |
| R5211 | T9818 | T9819 | nummod | Tween,20 |
| R5212 | T9819 | T9837 | dep | 20,phosphorylated |
| R5213 | T9820 | T9819 | prep | in,20 |
| R5214 | T9821 | T9822 | amod | Tris-buffered,saline |
| R5215 | T9822 | T9820 | pobj | saline,in |
| R5216 | T9823 | T9819 | punct | ),20 |
| R5217 | T9824 | T9819 | prep | at,20 |
| R5218 | T9825 | T9826 | compound | room,temperature |
| R5219 | T9826 | T9824 | pobj | temperature,at |
| R5220 | T9827 | T9819 | prep | for,20 |
| R5221 | T9828 | T9829 | nummod | 2,hours |
| R5222 | T9829 | T9827 | pobj | hours,for |
| R5223 | T9830 | T9819 | punct | ",",20 |
| R5224 | T9831 | T9833 | advmod | then,antibodies |
| R5225 | T9832 | T9833 | amod | primary,antibodies |
| R5226 | T9833 | T9819 | appos | antibodies,20 |
| R5227 | T9834 | T9833 | prep | against,antibodies |
| R5228 | T9835 | T9834 | pobj | Akt,against |
| R5229 | T9836 | T9819 | punct | ",",20 |
| R5230 | T9837 | T9810 | advcl | phosphorylated,skimmed |
| R5231 | T9838 | T9837 | dobj | Akt,phosphorylated |
| R5232 | T9839 | T9838 | cc | and,Akt |
| R5233 | T9840 | T9838 | conj | IκB-α,Akt |
| R5234 | T9841 | T9845 | punct | (,Inc. |
| R5235 | T9842 | T9845 | compound | Cell,Inc. |
| R5236 | T9843 | T9845 | compound | Signaling,Inc. |
| R5237 | T9844 | T9845 | compound | Technology,Inc. |
| R5238 | T9845 | T9838 | conj | Inc.,Akt |
| R5239 | T9846 | T9845 | punct | ",",Inc. |
| R5240 | T9847 | T9845 | npadvmod | Beverly,Inc. |
| R5241 | T9848 | T9847 | punct | ",",Beverly |
| R5242 | T9849 | T9847 | conj | MA,Beverly |
| R5243 | T9850 | T9849 | punct | ",",MA |
| R5244 | T9851 | T9849 | npadvmod | USA,MA |
| R5245 | T9852 | T9849 | punct | ),MA |
| R5246 | T9853 | T9845 | punct | ",",Inc. |
| R5247 | T9854 | T9837 | prep | diluted,phosphorylated |
| R5248 | T9855 | T9854 | npadvmod | 1:1000,diluted |
| R5249 | T9856 | T9855 | prep | in,1:1000 |
| R5250 | T9857 | T9858 | nummod | 5,% |
| R5251 | T9858 | T9859 | compound | %,BSA/TBS-T |
| R5252 | T9859 | T9856 | pobj | BSA/TBS-T,in |
| R5253 | T9860 | T9862 | punct | ",",added |
| R5254 | T9861 | T9862 | auxpass | were,added |
| R5255 | T9862 | T9806 | conj | added,preincubated |
| R5256 | T9863 | T9862 | cc | and,added |
| R5290 | T9897 | T9895 | pcomp | washing,After |
| R5291 | T9898 | T9902 | punct | ",",detected |
| R5292 | T9899 | T9900 | amod | hybridized,bands |
| R5293 | T9900 | T9902 | nsubjpass | bands,detected |
| R5294 | T9901 | T9902 | auxpass | were,detected |
| R5295 | T9902 | T9902 | ROOT | detected,detected |
| R5296 | T9903 | T9902 | prep | with,detected |
| R5297 | T9904 | T9906 | det | the,chemiluminescence |
| R5298 | T9905 | T9906 | amod | enhanced,chemiluminescence |
| R5299 | T9906 | T9903 | pobj | chemiluminescence,with |
| R5300 | T9907 | T9906 | punct | (,chemiluminescence |
| R5301 | T9908 | T9906 | appos | ECL,chemiluminescence |
| R5302 | T9909 | T9911 | punct | ),kit |
| R5303 | T9910 | T9911 | compound | detection,kit |
| R5304 | T9911 | T9906 | conj | kit,chemiluminescence |
| R5305 | T9912 | T9911 | cc | and,kit |
| R5306 | T9913 | T9914 | amod | Hyperfilm-ECL,reagents |
| R5307 | T9914 | T9911 | conj | reagents,kit |
| R5308 | T9915 | T9917 | punct | (,Pharmacia |
| R5309 | T9916 | T9917 | compound | Amersham,Pharmacia |
| R5310 | T9917 | T9914 | appos | Pharmacia,reagents |
| R5311 | T9918 | T9914 | punct | ),reagents |
| R5312 | T9919 | T9902 | punct | .,detected |
| R5079 | T9686 | T9688 | amod | Western,analysis |
| R5080 | T9687 | T9688 | compound | blot,analysis |
| R5081 | T9688 | T9692 | nsubj | analysis,phosphorylated |
| R5082 | T9689 | T9688 | prep | of,analysis |
| R5083 | T9690 | T9689 | pobj | Akt,of |
| R5084 | T9691 | T9688 | punct | ",",analysis |
| R5085 | T9692 | T9692 | ROOT | phosphorylated,phosphorylated |
| R5086 | T9693 | T9692 | dobj | Akt,phosphorylated |
| R5087 | T9694 | T9693 | cc | and,Akt |
| R5088 | T9695 | T9696 | compound | IκB-α,PBMC |
| R5089 | T9696 | T9693 | conj | PBMC,Akt |
| R5090 | T9697 | T9698 | auxpass | were,incubated |
| R5091 | T9698 | T9692 | conj | incubated,phosphorylated |
| R5092 | T9699 | T9698 | prep | with,incubated |
| R5093 | T9700 | T9699 | pobj | anti-CD3,with |
| R5094 | T9701 | T9705 | punct | (,ml |
| R5257 | T9864 | T9862 | conj | incubated,added |
| R5258 | T9865 | T9864 | advmod | overnight,incubated |
| R5259 | T9866 | T9864 | prep | at,incubated |
| R5260 | T9867 | T9868 | nummod | 4,° |
| R5261 | T9868 | T9866 | pobj | °,at |
| R5262 | T9869 | T9862 | nsubj | C,added |
| R5263 | T9870 | T9862 | punct | .,added |
| R5264 | T9871 | T9883 | prep | After,added |
| R5265 | T9872 | T9871 | pcomp | washing,After |
| R5266 | T9873 | T9874 | nummod | four,times |
| R5267 | T9874 | T9872 | dobj | times,washing |
| R5268 | T9875 | T9872 | prep | with,washing |
| R5269 | T9876 | T9875 | pobj | TBS-T,with |
| R5270 | T9877 | T9883 | punct | ",",added |
| R5271 | T9878 | T9881 | amod | horseradish,antibodies |
| R5272 | T9879 | T9881 | amod | peroxidase-conjugated,antibodies |
| R5273 | T9880 | T9881 | amod | secondary,antibodies |
| R5274 | T9881 | T9883 | nsubjpass | antibodies,added |
| R5275 | T9882 | T9883 | auxpass | were,added |
| R5276 | T9883 | T9883 | ROOT | added,added |
| R5277 | T9884 | T9883 | cc | and,added |
| R5278 | T9885 | T9883 | conj | allowed,added |
| R5279 | T9886 | T9887 | aux | to,incubate |
| R5280 | T9887 | T9885 | xcomp | incubate,allowed |
| R5281 | T9888 | T9887 | prep | for,incubate |
| R5282 | T9889 | T9890 | nummod | 1,hour |
| R5283 | T9890 | T9888 | pobj | hour,for |
| R5284 | T9891 | T9887 | prep | at,incubate |
| R5285 | T9892 | T9893 | compound | room,temperature |
| R5286 | T9893 | T9891 | pobj | temperature,at |
| R5287 | T9894 | T9883 | punct | .,added |
| R5288 | T9895 | T9902 | prep | After,detected |
| R5289 | T9896 | T9897 | nsubj | TBS-T,washing |
bionlp-st-ge-2016-test-tees
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9632 | 780-783 | Protein | denotes | Akt |
| T9631 | 30-44 | Phosphorylation | denotes | phosphorylated |
| T9630 | 53-58 | Protein | denotes | IκB-α |
| T9629 | 45-48 | Protein | denotes | Akt |
| T9628 | 25-28 | Protein | denotes | Akt |
| T9639 | 978-1000 | Protein | denotes | horseradish peroxidase |
| T9638 | 971-976 | Protein | denotes | TBS-T |
| T9637 | 785-799 | Phosphorylation | denotes | phosphorylated |
| T9636 | 785-799 | Phosphorylation | denotes | phosphorylated |
| T9635 | 785-799 | Phosphorylation | denotes | phosphorylated |
| T9634 | 808-813 | Protein | denotes | IκB-α |
| T9633 | 800-803 | Protein | denotes | Akt |
| R5066 | T9629 | T9631 | themeOf | Akt,phosphorylated |
| R5067 | T9632 | T9635 | themeOf | Akt,phosphorylated |
| R5068 | T9633 | T9636 | themeOf | Akt,phosphorylated |
| R5069 | T9634 | T9637 | themeOf | IκB-α,phosphorylated |
testone
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9204 | 785-799 | Phosphorylation | denotes | phosphorylated |
| T9203 | 30-44 | Phosphorylation | denotes | phosphorylated |
| T9202 | 808-813 | Protein | denotes | IκB-α |
| T9201 | 800-803 | Protein | denotes | Akt |
| T9200 | 780-783 | Protein | denotes | Akt |
| T9199 | 53-58 | Protein | denotes | IκB-α |
| T9198 | 45-48 | Protein | denotes | Akt |
| T9197 | 25-28 | Protein | denotes | Akt |
| R4822 | T9198 | T9203 | themeOf | Akt,phosphorylated |
| R4823 | T9199 | T9203 | themeOf | IκB-α,phosphorylated |
| R4824 | T9201 | T9204 | themeOf | Akt,phosphorylated |
| R4825 | T9202 | T9204 | themeOf | IκB-α,phosphorylated |