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PMC:1064873 / 7012-14987 JSONTXT

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LappsTest

Id Subject Object Predicate Lexical cue
T10612 7674-7679 Protein denotes STAT1
T10611 7596-7608 Protein denotes STAT protein
T10610 7537-7541 Protein denotes STAT
T10609 7307-7331 Protein denotes anti-rabbit IgG antibody
T10608 7133-7147 Protein denotes STAT3 antibody
T10607 7056-7091 Protein denotes STAT1 antibody, anti-STAT3 antibody
T10606 6987-7018 Protein denotes rabbit IgG) anti-STAT1 antibody
T10605 6787-6792 Protein denotes STAT3
T10604 6777-6782 Protein denotes STAT1
T10603 6313-6316 Protein denotes IgG
T10602 6264-6267 Protein denotes IgG
T10601 6233-6262 Protein denotes anti-IL-6 polyclonal antibody
T10600 6118-6122 Protein denotes CD4+
T10599 6089-6093 Protein denotes STAT
T10598 6081-6085 Protein denotes IL-6
T10597 5976-5980 Protein denotes IL-6
T10596 5966-5971 Protein denotes IL-10
T10595 5891-5895 Protein denotes CD4+
T9330 5806-5811 Protein denotes SOCS3
T9329 5731-5736 Protein denotes SOCS1
T9328 5654-5661 Protein denotes β-actin
T9327 5581-5588 Protein denotes β-actin
T9326 5521-5526 Protein denotes SOCS3
T9325 5511-5516 Protein denotes SOCS1
T9324 5358-5363 Protein denotes SOCS3
T9323 5289-5294 Protein denotes SOCS1
T9322 5222-5229 Protein denotes β-actin
T9321 4887-4905 Protein denotes Taq DNA polymerase
T9320 4596-4646 Protein denotes Molony murine leukemia virus reverse transcriptase
T9319 4420-4427 Protein denotes PB CD4+
T9007 4325-4329 Protein denotes IL-2
T9006 4315-4319 Protein denotes IFN-
T9005 4189-4218 Protein denotes recombinant cytokine proteins
T9004 4046-4050 Protein denotes CD4+
T9003 4014-4018 Protein denotes IL-2
T9002 4004-4008 Protein denotes IFN-
T9001 3981-3985 Protein denotes IL-2
T9000 3971-3975 Protein denotes IFN-
T8609 3790-3802 Protein denotes anti-CD4 mAb
T8608 3658-3682 Protein denotes IgG1 polyclonal antibody
T8607 3513-3524 Protein denotes R&D systems
T8606 3507-3511 Protein denotes IgG1
T8605 3489-3505 Protein denotes anti-IL-10R1 mAb
T8604 3346-3353 Protein denotes IL-10R1
T7989 3196-3209 Protein denotes anti-CD28 mAb
T7988 3179-3191 Protein denotes anti-CD3 mAb
T7987 3096-3100 Protein denotes IL-6
T7986 2976-2980 Protein denotes CD4+
T7985 2969-2974 Protein denotes IL-10
T7984 2936-2940 Protein denotes IL-6
T7983 2603-2616 Protein denotes anti-CD28 mAb
T7982 2519-2531 Protein denotes anti-CD3 mAb
T7981 2281-2285 Protein denotes CD4+
T7328 2198-2202 Protein denotes CD4+
T7327 1725-1730 Protein denotes DNase
T7326 1709-1720 Protein denotes collagenase
T7325 1575-1579 Protein denotes CD4+
T7324 1456-1464 Protein denotes anti-CD4
T7323 1391-1395 Protein denotes CD4+
T7322 1205-1209 Protein denotes CD4+
T6554 811-814 Protein denotes CRP
T6553 623-626 Protein denotes IgM
T6552 589-592 Protein denotes CRP
T6551 563-587 Protein denotes serum C-reactive protein

2_test

Id Subject Object Predicate Lexical cue
15535835-3358796-4156884 319-321 3358796 denotes 25
15535835-7306232-4156885 972-974 7306232 denotes 26
15535835-9182920-4156886 1644-1646 9182920 denotes 27
15535835-14726963-4156887 2790-2792 14726963 denotes 28

biosemtest

Id Subject Object Predicate Lexical cue
T9439 5596-5602 UP/O65315 denotes ion. T
T10710 7681-7686 UP/P42229 denotes hosop
T10859 7681-7686 UP/P42224 denotes hosop
T11972 7729-7738 UMLS/C0017262 denotes expressed
T11971 7711-7719 UMLS/C0002778 denotes analysis
T11970 7720-7724 UMLS/C1511726 denotes Data
T11969 7782-7786 UMLS/C0444504 denotes ean
T11968 7746-7750 UMLS/C0444504 denotes mean
T11967 7886-7891 UMLS/C0043572 denotes ann–W
T11966 7782-7786 UMLS/C2348143 denotes ean
T11965 7746-7750 UMLS/C2348143 denotes mean
T11964 7857-7863 UMLS/C1705429 denotes roups
T11963 7751-7756 UMLS/C1522609 denotes value
T11962 7857-7863 UMLS/C1705428 denotes roups
T11961 7868-7878 UMLS/C0521095 denotes etermined
T11960 7751-7756 UMLS/C0042295 denotes value
T11959 7903-7907 UMLS/C0022885 denotes t or
T11958 7782-7786 UMLS/C0876919 denotes ean
T11957 7746-7750 UMLS/C0876919 denotes mean
T11956 7853-7856 UMLS/C0205448 denotes wo
T11955 7857-7863 UMLS/C1522242 denotes roups
T11954 7805-7829 UMLS/C1709380 denotes tatistical significance
T11953 7903-7907 UMLS/C0183885 denotes t or
T11952 7903-7907 UMLS/C0013702 denotes t or
T11951 7711-7719 UMLS/C0936012 denotes analysis
T11950 7729-7738 UMLS/C1171362 denotes expressed
T11949 7857-7863 UMLS/C1552516 denotes roups
T11948 7760-7774 UMLS/C1710181 denotes tandard error
T11947 7729-7738 UMLS/C1515670 denotes expressed
T11946 7711-7719 UMLS/C1524024 denotes analysis
T11945 7903-7907 UMLS/C0039593 denotes t or
T11944 7857-7863 UMLS/C1552839 denotes roups
T11943 7915-7940 UMLS/C0871608 denotes coxon signed rank test. P
T11942 7782-7786 UMLS/C2347634 denotes ean
T11941 7746-7750 UMLS/C2347634 denotes mean
T11940 7699-7710 UMLS/C0038215 denotes Statistical
T11939 7857-7863 UMLS/C1257890 denotes roups
T11938 7751-7756 UMLS/C1704610 denotes value
T11937 7805-7829 UMLS/C0237881 denotes tatistical significance
T11936 7857-7863 UMLS/C0441833 denotes roups
T11935 7857-7863 UMLS/C1561557 denotes roups
T11934 7833-7844 UMLS/C1705242 denotes ifferences
T11933 7790-7799 UMLS/C1706992 denotes ox plots.
T11932 7782-7786 UMLS/C1704970 denotes ean
T11931 7746-7750 UMLS/C1704970 denotes mean
T11930 7903-7907 UMLS/C0392366 denotes t or
T11929 7833-7844 UMLS/C1705241 denotes ifferences
T11928 7751-7756 UMLS/C1554112 denotes value
T11927 7857-7863 UMLS/C0439745 denotes roups
T10926 6577-6581 UMLS/C0972569 denotes nd w
T10925 6302-6309 UMLS/C2587213 denotes ntrol g
T10924 7634-7644 UMLS/C0018873 denotes lls were u
T10923 7660-7676 UMLS/C1883676 denotes e control for ST
T10922 6525-6532 UMLS/C2347026 denotes s were
T10921 6839-6843 UMLS/C1704742 denotes Cell
T10920 6839-6843 UMLS/C0812225 denotes Cell
T10919 6922-6934 UMLS/C1442085 denotes ctions. Brie
T10918 6907-6921 UMLS/C1546933 denotes cturer's instr
T10917 6424-6427 UMLS/C0020259 denotes pH
T10916 7475-7480 UMLS/C0175723 denotes were
T10915 7329-7337 UMLS/C0003241 denotes dy dilut
T10914 7246-7254 UMLS/C0003241 denotes dy bindi
T10913 7145-7153 UMLS/C0003241 denotes dy, dilu
T10912 7089-7097 UMLS/C0003241 denotes dy, and
T10911 7068-7076 UMLS/C0003241 denotes dy, anti
T10910 7016-7024 UMLS/C0003241 denotes dy, anti
T10909 6981-6991 UMLS/C0003241 denotes dies (rabb
T10908 6112-6118 UMLS/C0205307 denotes ormal
T10907 7592-7598 UMLS/C1265611 denotes of ST
T10906 6525-6532 UMLS/C0870078 denotes s were
T10905 7586-7591 UMLS/C0439810 denotes amoun
T10904 6450-6458 UMLS/C0017861 denotes rol, 50
T10903 6365-6372 UMLS/C1704765 denotes acing c
T10902 6178-6183 UMLS/C1151254 denotes erum
T10901 6076-6081 UMLS/C1151254 denotes erum
T10900 6187-6201 UMLS/C0010454 denotes ulture medium
T10899 6022-6036 UMLS/C0010454 denotes ulture medium
T10898 7409-7412 UP/P02769 denotes nd
T10897 7241-7244 UP/P02769 denotes nti
T10896 6373-6378 UMLS/C0007634 denotes lls i
T10895 6006-6011 UMLS/C0007634 denotes ells
T10894 6155-6158 UMLS/C0439232 denotes in
T10893 5927-5930 UMLS/C0439232 denotes min
T10892 6302-6309 UMLS/C0332298 denotes ntrol g
T10891 6907-6921 UMLS/C1705426 denotes cturer's instr
T10890 6441-6444 UMLS/C1426244 denotes 10%
T10889 6390-6393 UMLS/C1426244 denotes ly
T10888 7255-7262 UMLS/C0243122 denotes g was d
T10887 6310-6314 UMLS/C1265549 denotes at I
T10886 6230-6234 UMLS/C1265549 denotes at a
T10885 7602-7614 UMLS/C1366753 denotes rotein. IFN-
T10884 7543-7547 UMLS/C1366753 denotes hosp
T10883 6090-6094 UMLS/C1366753 denotes TAT
T10882 7267-7275 UMLS/C0442726 denotes ed by ho
T10881 6803-6811 UMLS/C0442726 denotes ed using
T10805 5966-5971 UMLS/C1149215 denotes IL-10
T10804 6922-6934 UMLS/C0039401 denotes ctions. Brie
T10803 6335-6347 UMLS/C1881488 denotes ll lysates w
T10802 6178-6183 UMLS/C1546774 denotes erum
T10801 6076-6081 UMLS/C1546774 denotes erum
T10800 6429-6431 UMLS/C1304686 denotes 8]
T10799 7392-7400 UMLS/C0041415 denotes 20 with
T10798 7224-7232 UMLS/C0041415 denotes 20 with
T10797 6745-6753 UMLS/C0041415 denotes 20. Tyro
T10796 6441-6444 UMLS/C0272170 denotes 10%
T10795 6390-6393 UMLS/C0272170 denotes ly
T10794 6155-6158 UMLS/C1552937 denotes in
T10793 5927-5930 UMLS/C1552937 denotes min
T10792 6082-6086 UMLS/C1149241 denotes L-6
T10791 5976-5980 UMLS/C1149241 denotes IL-6
T10790 7681-7686 UMLS/C1335872 denotes hosop
T10789 7062-7067 UMLS/C1335872 denotes antib
T10788 6783-6788 UMLS/C1335872 denotes and S
T10787 6373-6378 UMLS/C1136359 denotes lls i
T10786 6006-6011 UMLS/C1136359 denotes ells
T10785 6066-6072 UMLS/C2348382 denotes ffect
T10784 7500-7512 UMLS/C0011321 denotes ometry using
T10783 7166-7177 UMLS/C0034866 denotes ended at 1/
T10782 7302-7312 UMLS/C0301869 denotes ated anti-
T10781 6701-6710 UMLS/C0349375 denotes ilk in Tr
T10780 5986-5993 UMLS/C0178587 denotes density
T10779 6591-6602 UMLS/C1547239 denotes erred to ni
T10778 6310-6314 UMLS/C1510458 denotes at I
T10777 6230-6234 UMLS/C1510458 denotes at a
T10776 6373-6378 UMLS/C1948049 denotes lls i
T10775 6006-6011 UMLS/C1948049 denotes ells
T10774 7267-7275 UMLS/C1511790 denotes ed by ho
T10773 6803-6811 UMLS/C1511790 denotes ed using
T10772 6066-6072 UMLS/C1280500 denotes ffect
T10771 6082-6086 UMLS/C1527132 denotes L-6
T10770 5976-5980 UMLS/C1527132 denotes IL-6
T10769 6155-6158 UMLS/C0702093 denotes in
T10768 5927-5930 UMLS/C0702093 denotes min
T10767 7409-7412 UMLS/C0005902 denotes nd
T10766 7241-7244 UMLS/C0005902 denotes nti
T10765 6922-6934 UMLS/C0220931 denotes ctions. Brie
T10764 6922-6934 UMLS/C1292796 denotes ctions. Brie
T10763 7338-7345 UMLS/C1948037 denotes d at 1/
T10762 7155-7162 UMLS/C1948037 denotes d as re
T10761 6829-6838 UMLS/C0470187 denotes ble kits
T10760 6577-6581 UMLS/C1382104 denotes nd w
T10759 6525-6532 UMLS/C0370003 denotes s were
T10758 6429-6431 UMLS/C0031526 denotes 8]
T10757 7543-7547 UMLS/C0205548 denotes hosp
T10756 6090-6094 UMLS/C0205548 denotes TAT
T10755 6429-6431 UMLS/C2363150 denotes 8]
T10754 6591-6602 UMLS/C0348011 denotes erred to ni
T10753 7622-7633 UMLS/C1948023 denotes ated Hela c
T10752 6137-6147 UMLS/C1948023 denotes timulated
T10751 5909-5919 UMLS/C1948023 denotes stimulated
T10750 5966-5971 UMLS/C1522550 denotes IL-10
T10749 6429-6431 UMLS/C0450409 denotes 8]
T10748 5966-5971 UMLS/C1334098 denotes IL-10
T10747 6429-6431 UMLS/C0450408 denotes 8]
T10746 6429-6431 UMLS/C0450407 denotes 8]
T10745 6302-6309 UMLS/C0009932 denotes ntrol g
T10744 6591-6602 UMLS/C1555583 denotes erred to ni
T10743 7409-7412 UMLS/C0036774 denotes nd
T10742 7241-7244 UMLS/C0036774 denotes nti
T10741 5869-5890 UMLS/C0949466 denotes Western blot analysis
T10740 6082-6086 UMLS/C0021760 denotes L-6
T10739 5976-5980 UMLS/C0021760 denotes IL-6
T10738 6818-6828 UMLS/C0680536 denotes cial avail
T10737 6606-6620 UMLS/C0600190 denotes ellulose membr
T10736 6082-6086 UMLS/C1334122 denotes L-6
T10735 5976-5980 UMLS/C1334122 denotes IL-6
T10734 7543-7547 UMLS/C1335960 denotes hosp
T10733 6090-6094 UMLS/C1335960 denotes TAT
T10732 6860-6870 UMLS/C0039421 denotes logy, Beve
T10731 7409-7412 UMLS/C1511233 denotes nd
T10730 7241-7244 UMLS/C1511233 denotes nti
T10729 7302-7312 UMLS/C0522529 denotes ated anti-
T10728 6591-6602 UMLS/C1540850 denotes erred to ni
T10727 7543-7547 UMLS/C1420304 denotes hosp
T10726 6090-6094 UMLS/C1420304 denotes TAT
T10725 6373-6378 UMLS/C1704653 denotes lls i
T10724 6006-6011 UMLS/C1704653 denotes ells
T10723 6591-6602 UMLS/C0040671 denotes erred to ni
T10722 6685-6692 UMLS/C0332206 denotes d with
T10721 6487-6502 UMLS/C0006247 denotes henol blue). Th
T10720 6577-6581 UMLS/C0017243 denotes nd w
T10719 7329-7337 UMLS/C0021027 denotes dy dilut
T10718 7246-7254 UMLS/C0021027 denotes dy bindi
T10717 7145-7153 UMLS/C0021027 denotes dy, dilu
T10716 7089-7097 UMLS/C0021027 denotes dy, and
T10715 7068-7076 UMLS/C0021027 denotes dy, anti
T10714 7016-7024 UMLS/C0021027 denotes dy, anti
T10713 6981-6991 UMLS/C0021027 denotes dies (rabb
T10712 6685-6692 UMLS/C1706084 denotes d with
T10711 6993-6999 UMLS/C1446777 denotes IgG)
T10709 7062-7067 UP/P42229 denotes antib
T10708 6783-6788 UP/P42229 denotes and S
T10707 6168-6174 UMLS/C1561511 denotes ctive
T10706 6168-6174 UMLS/C1561512 denotes ctive
T10705 6168-6174 UMLS/C1561513 denotes ctive
T10704 5938-5947 UMLS/C1444656 denotes indicated
T10703 7279-7301 UMLS/C0019941 denotes adish peroxidase-conju
T10702 7467-7474 UMLS/C0033684 denotes n bands
T10701 6517-6524 UMLS/C0033684 denotes n sampl
T10700 6591-6602 UMLS/C1299988 denotes erred to ni
T10699 7592-7598 UMLS/C1561574 denotes of ST
T10698 7255-7262 UMLS/C1167622 denotes g was d
T10697 6441-6444 UMLS/C0037506 denotes 10%
T10696 6390-6393 UMLS/C0037506 denotes ly
T10695 6885-6888 UMLS/C0041703 denotes cco
T10694 6294-6297 UMLS/C0041703 denotes A)
T10693 7529-7537 UMLS/C1524024 denotes is, and
T10692 6685-6692 UMLS/C1533157 denotes d with
T10691 6466-6480 UMLS/C0012789 denotes othreitol, 0.1
T10690 6168-6174 UMLS/C0205177 denotes ctive
T10689 6373-6378 UMLS/C1269647 denotes lls i
T10688 6006-6011 UMLS/C1269647 denotes ells
T10687 7123-7131 UMLS/C1880236 denotes ne 705 o
T10686 7046-7054 UMLS/C1880236 denotes ne 701 o
T10685 6329-6334 UMLS/C0444667 denotes ole c
T10684 6168-6174 UMLS/C2347179 denotes ctive
T10683 7441-7458 UMLS/C1450278 denotes uminescence syste
T10682 5966-5971 UMLS/C0085295 denotes IL-10
T10681 6112-6118 UMLS/C1704701 denotes ormal
T10680 6900-6902 UMLS/C0319022 denotes m
T10679 6603-6605 UMLS/C0319022 denotes ro
T10678 6051-6053 UMLS/C0319022 denotes o
T10677 7338-7345 UMLS/C1720119 denotes d at 1/
T10676 7155-7162 UMLS/C1720119 denotes d as re
T10675 6054-6061 UMLS/C0332128 denotes xamine
T10674 6685-6692 UMLS/C0028778 denotes d with
T10673 6112-6118 UMLS/C1550457 denotes ormal
T10672 6685-6692 UMLS/C0233660 denotes d with
T10671 6168-6174 UMLS/C1561510 denotes ctive
T10670 6168-6174 UMLS/C1561509 denotes ctive
T10669 6168-6174 UMLS/C1561508 denotes ctive
T10668 6168-6174 UMLS/C1561507 denotes ctive
T10667 7325-7328 UMLS/C0020852 denotes tib
T10666 7000-7003 UMLS/C0020852 denotes nti
T10665 6315-6318 UMLS/C0020852 denotes G (
T10664 6266-6269 UMLS/C0020852 denotes G;
T10663 6365-6372 UMLS/C1882509 denotes acing c
T10662 7548-7563 UMLS/C0031715 denotes orylation was c
T10661 6095-6110 UMLS/C0031715 denotes hosphorylation,
T10660 7255-7262 UMLS/C1145667 denotes g was d
T10659 6112-6118 UMLS/C1551394 denotes ormal
T10658 6993-6999 UMLS/C0034493 denotes IgG)
T10657 7255-7262 UMLS/C2349209 denotes g was d
T10656 6168-6174 UMLS/C1547419 denotes ctive
T10655 7123-7131 UMLS/C0041485 denotes ne 705 o
T10654 7046-7054 UMLS/C0041485 denotes ne 701 o
T10653 6839-6843 UMLS/C1705212 denotes Cell
T10652 6577-6581 UMLS/C1551386 denotes nd w
T10651 6302-6309 UMLS/C1882979 denotes ntrol g
T10880 6245-6264 UMLS/C0312586 denotes lyclonal antibody (
T10879 7422-7430 UMLS/C0443289 denotes ed using
T10878 6112-6118 UMLS/C2347086 denotes ormal
T10877 5948-5962 UMLS/C0086045 denotes concentrations
T10876 7529-7537 UMLS/C0936012 denotes is, and
T10875 6890-6899 UMLS/C0680240 denotes ing to th
T10874 6310-6314 UMLS/C0018019 denotes at I
T10873 6230-6234 UMLS/C0018019 denotes at a
T10872 6353-6361 UMLS/C1521827 denotes epared b
T10871 6907-6921 UMLS/C0947322 denotes cturer's instr
T10870 6441-6444 UMLS/C1419917 denotes 10%
T10869 6390-6393 UMLS/C1419917 denotes ly
T10868 7586-7591 UMLS/C0439175 denotes amoun
T10867 7139-7144 UMLS/C1367307 denotes antib
T10866 6793-6798 UMLS/C1367307 denotes was d
T10865 6112-6118 UMLS/C0439166 denotes ormal
T10864 6401-6407 UMLS/C0006353 denotes fer (6
T10863 7409-7412 UMLS/C0487992 denotes nd
T10862 7241-7244 UMLS/C0487992 denotes nti
T10861 6302-6309 UMLS/C0243148 denotes ntrol g
T10860 6450-6458 UMLS/C0768477 denotes rol, 50
T10858 7062-7067 UP/P42224 denotes antib
T10857 6783-6788 UP/P42224 denotes and S
T10856 7441-7458 UMLS/C0162524 denotes uminescence syste
T10855 7475-7480 UMLS/C0439645 denotes were
T10854 7375-7381 UMLS/C0036082 denotes with
T10853 7207-7213 UMLS/C0036082 denotes with
T10852 6728-6734 UMLS/C0036082 denotes with
T10851 6907-6921 UMLS/C1548599 denotes cturer's instr
T10850 6839-6843 UMLS/C1690540 denotes Cell
T10849 6755-6779 UMLS/C1519726 denotes ne phosphorylation of ST
T10848 6178-6183 UMLS/C1550100 denotes erum
T10847 6076-6081 UMLS/C1550100 denotes erum
T10846 6845-6859 UMLS/C0037083 denotes ignaling Techn
T10845 7459-7465 UMLS/C1553451 denotes . Prot
T10844 6839-6843 UMLS/C1553450 denotes Cell
T10843 6591-6602 UMLS/C1705822 denotes erred to ni
T10842 6962-6971 UMLS/C1439852 denotes ted with
T10841 7459-7465 UMLS/C0449913 denotes . Prot
T10840 6302-6309 UMLS/C1547100 denotes ntrol g
T10839 7139-7144 UMLS/C0253050 denotes antib
T10838 6793-6798 UMLS/C0253050 denotes was d
T10837 7255-7262 UMLS/C1689898 denotes g was d
T10836 6112-6118 UMLS/C1873497 denotes ormal
T10835 6922-6934 UMLS/C0302828 denotes ctions. Brie
T10834 6642-6657 UMLS/C0454842 denotes ghamshire, UK),
T10833 7459-7465 UMLS/C1704459 denotes . Prot
T10832 7329-7337 UMLS/C1621287 denotes dy dilut
T10831 7246-7254 UMLS/C1621287 denotes dy bindi
T10830 7145-7153 UMLS/C1621287 denotes dy, dilu
T10829 7089-7097 UMLS/C1621287 denotes dy, and
T10828 7068-7076 UMLS/C1621287 denotes dy, anti
T10827 7016-7024 UMLS/C1621287 denotes dy, anti
T10826 6981-6991 UMLS/C1621287 denotes dies (rabb
T10825 7325-7328 UMLS/C0202087 denotes tib
T10824 7000-7003 UMLS/C0202087 denotes nti
T10823 6315-6318 UMLS/C0202087 denotes G (
T10822 6266-6269 UMLS/C0202087 denotes G;
T10821 6168-6174 UMLS/C1553875 denotes ctive
T10820 6124-6131 UMLS/C0039194 denotes cells
T10819 5896-5903 UMLS/C0039194 denotes T cells
T10818 6525-6532 UMLS/C0441621 denotes s were
T10817 6429-6431 UMLS/C0020283 denotes 8]
T10816 7568-7576 UMLS/C1707455 denotes ed with
T10815 6302-6309 UMLS/C1550141 denotes ntrol g
T10814 7475-7480 UMLS/C0185014 denotes were
T10813 6178-6183 UMLS/C0229671 denotes erum
T10812 6076-6081 UMLS/C0229671 denotes erum
T10811 6591-6602 UMLS/C0728827 denotes erred to ni
T10810 5948-5962 UMLS/C1446561 denotes concentrations
T10809 7543-7547 UMLS/C1548425 denotes hosp
T10808 6090-6094 UMLS/C1548425 denotes TAT
T10807 7529-7537 UMLS/C0002778 denotes is, and
T10806 6538-6550 UMLS/C1979893 denotes onated on 10
T9722 4863-4871 UMLS/C1114821 denotes reaction
T9721 5081-5088 UMLS/C2347026 denotes samples
T9720 4476-4479 UMLS/C0812225 denotes kit
T9719 4459-4462 UMLS/C0812225 denotes kit
T9718 4476-4479 UMLS/C1704742 denotes kit
T9717 4459-4462 UMLS/C1704742 denotes kit
T9716 5670-5676 UP/P24902 denotes GGCGCC
T9715 5596-5602 UP/P24902 denotes ion. T
T9714 5230-5236 UP/P24902 denotes at 95
T9713 5076-5080 UMLS/C0006556 denotes cDNA
T9712 5056-5060 UMLS/C0006556 denotes cDNA
T9711 4555-4559 UMLS/C0006556 denotes cDNA
T9710 4541-4553 UMLS/C1442085 denotes instructions
T9709 5670-5676 UP/P30161 denotes GGCGCC
T9708 5596-5602 UP/P30161 denotes ion. T
T9707 5230-5236 UP/P30161 denotes at 95
T9706 5008-5019 UMLS/C0011900 denotes Diagnostics
T9705 4805-4816 UMLS/C0011900 denotes Diagnostics
T9704 4526-4540 UMLS/C1546933 denotes manufacturer's
T9703 5670-5676 UP/P11426 denotes GGCGCC
T9702 5596-5602 UP/P11426 denotes ion. T
T9701 5230-5236 UP/P11426 denotes at 95
T9700 5670-5676 UP/Q11212 denotes GGCGCC
T9699 5596-5602 UP/Q11212 denotes ion. T
T9698 5230-5236 UP/Q11212 denotes at 95
T9697 4710-4717 UMLS/C0072149 denotes Promega
T9696 5619-5626 UMLS/C0439780 denotes verse p
T9695 5433-5438 UMLS/C1511572 denotes were
T9694 5162-5168 UMLS/C1511572 denotes les: a
T9693 5670-5676 UP/Q92192 denotes GGCGCC
T9692 5596-5602 UP/Q92192 denotes ion. T
T9691 5230-5236 UP/Q92192 denotes at 95
T9690 5081-5088 UMLS/C0870078 denotes samples
T9689 5670-5676 UP/P91754 denotes GGCGCC
T9688 5596-5602 UP/P91754 denotes ion. T
T9687 5230-5236 UP/P91754 denotes at 95
T9686 4581-4586 UMLS/C0439810 denotes total
T9685 4382-4387 UMLS/C0439810 denotes Total
T9684 4876-4886 UMLS/C0332256 denotes containing
T9683 5821-5826 UMLS/C1426212 denotes CGGCA
T9682 5534-5539 UMLS/C1426212 denotes ion w
T9681 5371-5376 UMLS/C1426212 denotes icati
T9680 4625-4646 UP/P21329 denotes reverse transcriptase
T9679 4449-4458 UMLS/C0204727 denotes isolation
T9678 4346-4355 UMLS/C0204727 denotes Isolation
T9677 5670-5676 UP/P51775 denotes GGCGCC
T9676 5596-5602 UP/P51775 denotes ion. T
T9675 5230-5236 UP/P51775 denotes at 95
T9674 5670-5676 UP/P20904 denotes GGCGCC
T9673 5596-5602 UP/P20904 denotes ion. T
T9672 5230-5236 UP/P20904 denotes at 95
T9671 5655-5662 UMLS/C0332283 denotes -actin,
T9670 5670-5676 UP/P13363 denotes GGCGCC
T9669 5596-5602 UP/P13363 denotes ion. T
T9668 5230-5236 UP/P13363 denotes at 95
T9667 5670-5676 UP/P53498 denotes GGCGCC
T9666 5596-5602 UP/P53498 denotes ion. T
T9665 5230-5236 UP/P53498 denotes at 95
T9664 4388-4396 UMLS/C0007634 denotes cellular
T9663 5655-5662 UMLS/C0332282 denotes -actin,
T9662 5746-5751 UMLS/C1426208 denotes CCTTC
T9661 5524-5529 UMLS/C1426208 denotes S3 ex
T9660 5299-5304 UMLS/C1426208 denotes at 9
T9659 5670-5676 UP/P26182 denotes GGCGCC
T9658 5596-5602 UP/P26182 denotes ion. T
T9657 5230-5236 UP/P26182 denotes at 95
T9656 5025-5033 UMLS/C1552740 denotes specific
T9655 4928-4936 UMLS/C1552740 denotes specific
T9654 5439-5445 UMLS/C0237753 denotes btaine
T9653 4977-4980 UMLS/C0012854 denotes DNA
T9652 4876-4886 UMLS/C2700400 denotes containing
T9651 5670-5676 UP/P14235 denotes GGCGCC
T9650 5596-5602 UP/P14235 denotes ion. T
T9649 5230-5236 UP/P14235 denotes at 95
T9648 5670-5676 UP/P60010 denotes GGCGCC
T9647 5596-5602 UP/P60010 denotes ion. T
T9646 5230-5236 UP/P60010 denotes at 95
T9645 5670-5676 UMLS/C0001271 denotes GGCGCC
T9644 5596-5602 UMLS/C0001271 denotes ion. T
T9643 5230-5236 UMLS/C0001271 denotes at 95
T9642 4526-4540 UMLS/C1705426 denotes manufacturer's
T9641 5467-5474 UMLS/C0599882 denotes analys
T9640 5582-5590 UMLS/C0080103 denotes -actin e
T9639 4876-4886 UMLS/C1549781 denotes containing
T9638 4863-4871 UMLS/C0443286 denotes reaction
T9637 5570-5581 UMLS/C1882115 denotes elative to
T9636 5481-5489 UMLS/C0936012 denotes then per
T9635 4509-4518 UMLS/C0680240 denotes according
T9634 4625-4646 UMLS/C0035379 denotes reverse transcriptase
T9633 5670-5676 UP/Q8X119 denotes GGCGCC
T9632 5596-5602 UP/Q8X119 denotes ion. T
T9631 5230-5236 UP/Q8X119 denotes at 95
T9630 5670-5676 UP/P10365 denotes GGCGCC
T9629 5596-5602 UP/P10365 denotes ion. T
T9628 5230-5236 UP/P10365 denotes at 95
T9627 4625-4646 UP/P23070 denotes reverse transcriptase
T9626 4526-4540 UMLS/C0947322 denotes manufacturer's
T9625 5230-5236 UP/P60009 denotes at 95
T9624 4449-4458 UMLS/C1547129 denotes isolation
T9623 4346-4355 UMLS/C1547129 denotes Isolation
T9622 5670-5676 UP/P90689 denotes GGCGCC
T9621 5596-5602 UP/P90689 denotes ion. T
T9620 5230-5236 UP/P90689 denotes at 95
T9619 5670-5676 UP/P53499 denotes GGCGCC
T9618 5596-5602 UP/P53499 denotes ion. T
T9617 5230-5236 UP/P53499 denotes at 95
T9616 5670-5676 UP/O74258 denotes GGCGCC
T9615 5596-5602 UP/O74258 denotes ion. T
T9614 5230-5236 UP/O74258 denotes at 95
T9613 4405-4414 UMLS/C1555707 denotes extracted
T9612 5047-5052 UMLS/C1883712 denotes added
T9611 4887-4905 UMLS/C2257065 denotes Taq DNA polymerase
T9610 4428-4435 UMLS/C0039194 denotes T cells
T9609 5118-5124 UMLS/C0456692 denotes 0 min,
T9608 5081-5088 UMLS/C0441621 denotes samples
T9607 5025-5033 UMLS/C0205369 denotes specific
T9606 4928-4936 UMLS/C0205369 denotes specific
T9605 5514-5520 UMLS/C0456079 denotes S1 and
T9604 5582-5590 UMLS/C0205345 denotes -actin e
T9603 5670-5676 UP/P45521 denotes GGCGCC
T9602 5596-5602 UP/P45521 denotes ion. T
T9601 5230-5236 UP/P45521 denotes at 95
T9600 5670-5676 UP/P48465 denotes GGCGCC
T9599 5596-5602 UP/P48465 denotes ion. T
T9598 5230-5236 UP/P48465 denotes at 95
T9597 5670-5676 UP/P26183 denotes GGCGCC
T9596 5596-5602 UP/P26183 denotes ion. T
T9595 5230-5236 UP/P26183 denotes at 95
T9594 5670-5676 UP/P50138 denotes GGCGCC
T9593 5596-5602 UP/P50138 denotes ion. T
T9592 5230-5236 UP/P50138 denotes at 95
T9591 5481-5489 UMLS/C0002778 denotes then per
T9590 4541-4553 UMLS/C0039401 denotes instructions
T9589 5419-5425 UMLS/C1522609 denotes cycle
T9588 4872-4875 UMLS/C1720722 denotes mix
T9587 5514-5520 UMLS/C0441889 denotes S1 and
T9586 4625-4646 UP/Q95SX7 denotes reverse transcriptase
T9585 5670-5676 UP/Q2U7A3 denotes GGCGCC
T9584 5596-5602 UP/Q2U7A3 denotes ion. T
T9583 5230-5236 UP/Q2U7A3 denotes at 95
T9582 5603-5613 UMLS/C0185117 denotes e forward
T9581 5540-5550 UMLS/C0185117 denotes re determi
T9580 5451-5459 UMLS/C1706701 denotes a melti
T9579 5670-5676 UP/P10989 denotes GGCGCC
T9578 5596-5602 UP/P10989 denotes ion. T
T9577 5230-5236 UP/P10989 denotes at 95
T9576 4405-4414 UMLS/C0185115 denotes extracted
T9575 5670-5676 UP/Q8SWN8 denotes GGCGCC
T9574 5596-5602 UP/Q8SWN8 denotes ion. T
T9573 5230-5236 UP/Q8SWN8 denotes at 95
T9572 5631-5646 UMLS/C1953384 denotes s were as follo
T9571 5670-5676 UP/P68555 denotes GGCGCC
T9570 5596-5602 UP/P68555 denotes ion. T
T9569 5230-5236 UP/P68555 denotes at 95
T9568 4846-4857 UMLS/C1280521 denotes capillaries
T9567 5670-5676 UP/P53500 denotes GGCGCC
T9566 5596-5602 UP/P53500 denotes ion. T
T9565 5230-5236 UP/P53500 denotes at 95
T9564 5670-5676 UP/P60011 denotes GGCGCC
T9563 5596-5602 UP/P60011 denotes ion. T
T9562 5230-5236 UP/P60011 denotes at 95
T9561 5047-5052 UMLS/C1524062 denotes added
T9560 5451-5459 UMLS/C1301820 denotes a melti
T9559 4541-4553 UMLS/C0220931 denotes instructions
T9558 4541-4553 UMLS/C1292796 denotes instructions
T9557 5670-5676 UP/P53476 denotes GGCGCC
T9556 5596-5602 UP/P53476 denotes ion. T
T9438 5230-5236 UP/O65315 denotes at 95
T9437 5670-5676 UP/O16808 denotes GGCGCC
T9436 5596-5602 UP/O16808 denotes ion. T
T9435 5230-5236 UP/O16808 denotes at 95
T9434 4449-4458 UMLS/C0205409 denotes isolation
T9433 5392-5398 UMLS/C0205134 denotes fluor
T9432 4346-4355 UMLS/C0205409 denotes Isolation
T9431 4664-4673 UMLS/C0454845 denotes Cleveland
T9430 5439-5445 UMLS/C0449788 denotes btaine
T9429 5670-5676 UP/Q9UVZ8 denotes GGCGCC
T9428 5596-5602 UP/Q9UVZ8 denotes ion. T
T9427 5230-5236 UP/Q9UVZ8 denotes at 95
T9426 5670-5676 UP/Q92193 denotes GGCGCC
T9425 5596-5602 UP/Q92193 denotes ion. T
T9424 5670-5676 UP/P45520 denotes GGCGCC
T9423 5596-5602 UP/P45520 denotes ion. T
T9422 5230-5236 UP/P45520 denotes at 95
T9421 5230-5236 UP/Q92193 denotes at 95
T9420 5378-5391 UMLS/C1705759 denotes curves of th
T9419 5670-5676 UP/O65316 denotes GGCGCC
T9418 5596-5602 UP/O65316 denotes ion. T
T9417 5230-5236 UP/O65316 denotes at 95
T9416 5439-5445 UMLS/C1709287 denotes btaine
T9415 4587-4590 UMLS/C1325819 denotes RNA
T9414 4445-4448 UMLS/C1325819 denotes RNA
T9413 4603-4624 UMLS/C0026626 denotes murine leukemia virus
T9412 4397-4400 UMLS/C1325819 denotes RNA
T9411 5670-5676 UP/P60009 denotes GGCGCC
T9410 5406-5418 UMLS/C0016315 denotes values versu
T9409 5596-5602 UP/P60009 denotes ion. T
T9408 4738-4751 UMLS/C1709846 denotes Real-time PCR
T9407 4368-4381 UMLS/C1709846 denotes real-time PCR
T9406 5670-5676 UP/O13419 denotes GGCGCC
T9405 5596-5602 UP/O13419 denotes ion. T
T9404 5230-5236 UP/O13419 denotes at 95
T9403 4732-4735 UMLS/C0041703 denotes USA
T9402 4679-4682 UMLS/C0041703 denotes USA
T9401 4503-4506 UMLS/C0041703 denotes USA
T9400 4840-4845 UMLS/C1947936 denotes glass
T9399 5481-5489 UMLS/C1524024 denotes then per
T9398 4981-4987 UMLS/C0444649 denotes Master
T9397 4449-4458 UMLS/C1608300 denotes isolation
T9396 4346-4355 UMLS/C1608300 denotes Isolation
T9395 5670-5676 UP/P17128 denotes GGCGCC
T9394 5596-5602 UP/P17128 denotes ion. T
T9393 5230-5236 UP/P17128 denotes at 95
T9392 4449-4458 UMLS/C1608299 denotes isolation
T9391 4346-4355 UMLS/C1608299 denotes Isolation
T9390 5419-5425 UMLS/C1704610 denotes cycle
T9389 5047-5052 UMLS/C1720086 denotes added
T9388 4449-4458 UMLS/C1548355 denotes isolation
T9387 4346-4355 UMLS/C1548355 denotes Isolation
T9386 5670-5676 UP/Q39596 denotes GGCGCC
T9385 5596-5602 UP/Q39596 denotes ion. T
T9384 5230-5236 UP/Q39596 denotes at 95
T9383 5670-5676 UP/P53689 denotes GGCGCC
T9382 5596-5602 UP/P53689 denotes ion. T
T9381 5230-5236 UP/P53689 denotes at 95
T9380 5061-5070 UMLS/C0079240 denotes dilutions
T9379 4625-4646 UP/Q9NBX4 denotes reverse transcriptase
T9378 4840-4845 UMLS/C0025611 denotes glass
T9377 5582-5590 UMLS/C1546849 denotes -actin e
T9376 5591-5593 UMLS/C0319022 denotes pr
T9375 5053-5055 UMLS/C0319022 denotes to
T9374 4519-4521 UMLS/C0319022 denotes to
T9373 4359-4363 UMLS/C0035696 denotes mRNA
T9372 5670-5676 UP/Q9UVX4 denotes GGCGCC
T9371 5596-5602 UP/Q9UVX4 denotes ion. T
T9370 5230-5236 UP/Q9UVX4 denotes at 95
T9369 5670-5676 UP/Q9P4D1 denotes GGCGCC
T9368 5596-5602 UP/Q9P4D1 denotes ion. T
T9367 5230-5236 UP/Q9P4D1 denotes at 95
T9366 5008-5019 UMLS/C1547424 denotes Diagnostics
T9365 4805-4816 UMLS/C1547424 denotes Diagnostics
T9364 5034-5041 UMLS/C0206415 denotes primers
T9363 4701-4708 UMLS/C0206415 denotes primers
T9362 4587-4590 UMLS/C0035668 denotes RNA
T9361 4445-4448 UMLS/C0035668 denotes RNA
T9360 4397-4400 UMLS/C0035668 denotes RNA
T9359 4625-4646 UP/P21328 denotes reverse transcriptase
T9358 4476-4479 UMLS/C1705212 denotes kit
T9357 4459-4462 UMLS/C1705212 denotes kit
T9356 4489-4497 UMLS/C1217267 denotes Valencia
T9067 4066-4074 UMLS/C1879489 denotes asured i
T9066 4328-4332 UMLS/C1149229 denotes 2 we
T9065 4016-4020 UMLS/C1149229 denotes -2 i
T9064 3982-3986 UMLS/C1149229 denotes L-2
T9063 4267-4281 UMLS/C1548599 denotes nufacturer's p
T9062 4032-4044 UMLS/C1550101 denotes pernatants o
T9061 4024-4031 UMLS/C0220814 denotes lture s
T9060 4267-4281 UMLS/C1546933 denotes nufacturer's p
T9059 4212-4220 UMLS/C0033684 denotes oteins (
T9058 3955-3966 UMLS/C0020980 denotes Immunoassay
T9057 4328-4332 UMLS/C0879590 denotes 2 we
T9056 4016-4020 UMLS/C0879590 denotes -2 i
T9055 3982-3986 UMLS/C0879590 denotes L-2
T9054 4024-4031 UMLS/C0010453 denotes lture s
T9053 4078-4087 UMLS/C0205173 denotes plicate b
T9052 4173-4182 UMLS/C1511790 denotes tection m
T9051 4138-4146 UMLS/C1552740 denotes ecific c
T9050 4282-4290 UMLS/C0442711 denotes otocol.
T9049 4282-4290 UMLS/C2348563 denotes otocol.
T9048 4066-4074 UMLS/C0242485 denotes asured i
T9047 4066-4074 UMLS/C0079809 denotes asured i
T9046 4267-4281 UMLS/C1705426 denotes nufacturer's p
T9045 4328-4332 UMLS/C1522405 denotes 2 we
T9044 4016-4020 UMLS/C1522405 denotes -2 i
T9043 3982-3986 UMLS/C1522405 denotes L-2
T9042 4296-4312 UMLS/C2718050 denotes tection limits f
T9041 4260-4262 UMLS/C0319022 denotes t
T9040 4066-4074 UMLS/C0444706 denotes asured i
T9039 4095-4107 UMLS/C0392762 denotes antitative s
T9038 3987-4001 UMLS/C0086045 denotes oncentrations
T9037 4282-4290 UMLS/C1507394 denotes otocol.
T9036 4250-4259 UMLS/C0680240 denotes cording t
T9035 4024-4031 UMLS/C2242979 denotes lture s
T9034 4191-4211 UMLS/C1524094 denotes combinant cytokine p
T9033 4129-4137 UMLS/C1524094 denotes tokine-s
T9032 4117-4122 UMLS/C0014441 denotes ISA u
T9031 4282-4290 UMLS/C2349977 denotes otocol.
T9030 4053-4060 UMLS/C0039194 denotes cells w
T9029 4138-4146 UMLS/C0205369 denotes ecific c
T9028 4267-4281 UMLS/C0947322 denotes nufacturer's p
T9027 4024-4031 UMLS/C0430400 denotes lture s
T9026 4129-4137 UMLS/C0079189 denotes tokine-s
T9025 4328-4332 UMLS/C0021756 denotes 2 we
T9024 4016-4020 UMLS/C0021756 denotes -2 i
T9023 3982-3986 UMLS/C0021756 denotes L-2
T9022 4282-4290 UMLS/C1522729 denotes otocol.
T9021 3987-4001 UMLS/C1446561 denotes oncentrations
T9555 5230-5236 UP/P53476 denotes at 95
T9554 4388-4396 UMLS/C0178539 denotes cellular
T9553 5061-5070 UMLS/C1948037 denotes dilutions
T9552 4988-5000 UMLS/C0386577 denotes SYBR Green I
T9551 4937-4949 UMLS/C0386577 denotes SYBR Green I
T9550 5670-5676 UP/P53502 denotes GGCGCC
T9549 5596-5602 UP/P53502 denotes ion. T
T9548 5230-5236 UP/P53502 denotes at 95
T9547 5081-5088 UMLS/C0370003 denotes samples
T9546 4787-4797 UMLS/C0348000 denotes Instrument
T9545 4471-4475 UMLS/C0445542 denotes Mini
T9544 4846-4857 UMLS/C0006901 denotes capillaries
T9543 4625-4646 UP/P23072 denotes reverse transcriptase
T9542 4581-4586 UMLS/C0439175 denotes total
T9541 4382-4387 UMLS/C0439175 denotes Total
T9540 4895-4913 UMLS/C0012892 denotes polymerase and DNA
T9539 5582-5590 UMLS/C1547039 denotes -actin e
T9538 5670-5676 UP/Q39758 denotes GGCGCC
T9537 5596-5602 UP/Q39758 denotes ion. T
T9536 5230-5236 UP/Q39758 denotes at 95
T9535 5670-5676 UP/O00937 denotes GGCGCC
T9534 5596-5602 UP/O00937 denotes ion. T
T9533 5230-5236 UP/O00937 denotes at 95
T9532 4846-4857 UMLS/C0935624 denotes capillaries
T9531 5008-5019 UMLS/C0348026 denotes Diagnostics
T9530 5670-5676 UP/Q99023 denotes GGCGCC
T9529 4805-4816 UMLS/C0348026 denotes Diagnostics
T9528 5467-5474 UMLS/C1881888 denotes analys
T9527 5596-5602 UP/Q99023 denotes ion. T
T9526 5230-5236 UP/Q99023 denotes at 95
T9525 5670-5676 UP/P80709 denotes GGCGCC
T9524 5670-5676 UP/P53455 denotes GGCGCC
T9523 5596-5602 UP/P53455 denotes ion. T
T9522 5230-5236 UP/P53455 denotes at 95
T9521 5596-5602 UP/P80709 denotes ion. T
T9520 5230-5236 UP/P80709 denotes at 95
T9519 5670-5676 UP/P78711 denotes GGCGCC
T9518 5596-5602 UP/P78711 denotes ion. T
T9517 5230-5236 UP/P78711 denotes at 95
T9516 5670-5676 UP/Q75D00 denotes GGCGCC
T9515 5670-5676 UP/O81221 denotes GGCGCC
T9514 5596-5602 UP/O81221 denotes ion. T
T9513 5596-5602 UP/Q75D00 denotes ion. T
T9512 5230-5236 UP/Q75D00 denotes at 95
T9511 4405-4414 UMLS/C1705851 denotes extracted
T9510 4564-4575 UMLS/C1883254 denotes synthesized
T9509 5034-5041 UMLS/C1279901 denotes primers
T9508 5230-5236 UP/O81221 denotes at 95
T9507 4701-4708 UMLS/C1279901 denotes primers
T9506 5419-5425 UMLS/C0042295 denotes cycle
T9505 5670-5676 UP/P53477 denotes GGCGCC
T9504 5596-5602 UP/P53477 denotes ion. T
T9503 5230-5236 UP/P53477 denotes at 95
T9502 5670-5676 UP/Q6TCF2 denotes GGCGCC
T9501 5596-5602 UP/Q6TCF2 denotes ion. T
T9500 5230-5236 UP/Q6TCF2 denotes at 95
T9499 4651-4662 UMLS/C0205474 denotes Biochemical
T9498 5499-5508 UMLS/C0884358 denotes e levels
T9497 4526-4540 UMLS/C1548599 denotes manufacturer's
T9496 4476-4479 UMLS/C1690540 denotes kit
T9495 4459-4462 UMLS/C1690540 denotes kit
T9494 4756-4765 UMLS/C0884358 denotes performed
T9493 5670-5676 UP/Q9UVF3 denotes GGCGCC
T9492 5596-5602 UP/Q9UVF3 denotes ion. T
T9491 5670-5676 UP/P53491 denotes GGCGCC
T9490 5596-5602 UP/P53491 denotes ion. T
T9489 5230-5236 UP/P53491 denotes at 95
T9488 5378-5391 UMLS/C1517480 denotes curves of th
T9487 5230-5236 UP/Q9UVF3 denotes at 95
T9486 4449-4458 UMLS/C0220862 denotes isolation
T9485 4476-4479 UMLS/C1553450 denotes kit
T9484 4346-4355 UMLS/C0220862 denotes Isolation
T9483 4459-4462 UMLS/C1553450 denotes kit
T9482 4828-4835 UMLS/C0017480 denotes Germany
T9481 5670-5676 UP/O65314 denotes GGCGCC
T9480 5596-5602 UP/O65314 denotes ion. T
T9479 5230-5236 UP/O65314 denotes at 95
T9478 5034-5041 UMLS/C0205435 denotes primers
T9477 4701-4708 UMLS/C0205435 denotes primers
T9476 5670-5676 UP/Q24733 denotes GGCGCC
T9475 5596-5602 UP/Q24733 denotes ion. T
T9474 5230-5236 UP/Q24733 denotes at 95
T9473 4625-4646 UP/P23071 denotes reverse transcriptase
T9472 5655-5662 UMLS/C1719822 denotes -actin,
T9471 4840-4845 UMLS/C0017596 denotes glass
T9470 4950-4953 UMLS/C0013343 denotes dye
T9469 5670-5676 UMLS/C0439681 denotes GGCGCC
T9468 5596-5602 UMLS/C0439681 denotes ion. T
T9467 5230-5236 UMLS/C0439681 denotes at 95
T9466 5439-5445 UMLS/C1554106 denotes btaine
T9465 4872-4875 UMLS/C0205430 denotes mix
T9464 4887-4905 UMLS/C0075813 denotes Taq DNA polymerase
T9463 5419-5425 UMLS/C1554112 denotes cycle
T9462 5378-5391 UMLS/C1521871 denotes curves of th
T9461 5670-5676 UP/Q05214 denotes GGCGCC
T9460 5596-5602 UP/Q05214 denotes ion. T
T9459 5230-5236 UP/Q05214 denotes at 95
T9458 4840-4845 UMLS/C0015421 denotes glass
T9457 5603-5613 UMLS/C0017262 denotes e forward
T9456 5540-5550 UMLS/C0017262 denotes re determi
T9455 5499-5508 UMLS/C1550369 denotes e levels
T9454 4756-4765 UMLS/C1550369 denotes performed
T9453 5670-5676 UP/A5DQP9 denotes GGCGCC
T9452 5596-5602 UP/A5DQP9 denotes ion. T
T9451 5230-5236 UP/A5DQP9 denotes at 95
T9450 4977-4980 UMLS/C1325786 denotes DNA
T9449 5670-5676 UP/O17320 denotes GGCGCC
T9448 5596-5602 UP/O17320 denotes ion. T
T9447 5230-5236 UP/O17320 denotes at 95
T9446 5670-5676 UP/P81085 denotes GGCGCC
T9445 5596-5602 UP/P81085 denotes ion. T
T9444 5230-5236 UP/P81085 denotes at 95
T9443 5556-5566 UMLS/C0521095 denotes normalizi
T9442 5475-5480 UMLS/C0205134 denotes s was
T9441 4541-4553 UMLS/C0302828 denotes instructions
T9440 5670-5676 UP/O65315 denotes GGCGCC
T8692 3780-3790 UMLS/C0522529 denotes onjugated
T8691 3632-3642 UMLS/C0522529 denotes onjugated
T8690 3354-3364 UMLS/C0017262 denotes expression
T8689 3884-3892 UMLS/C0002778 denotes nalysis
T8688 3333-3341 UMLS/C0002778 denotes analysis
T8687 3835-3841 UMLS/C1548982 denotes ashed
T8686 3824-3829 UMLS/C1704653 denotes ells
T8685 3735-3740 UMLS/C1704653 denotes ells
T8684 3386-3391 UMLS/C1704653 denotes ells
T8683 3897-3906 UMLS/C1550369 denotes erformed
T8682 3573-3580 UMLS/C2587213 denotes ontrol
T8681 3461-3471 UMLS/C0522534 denotes aturating
T8680 3627-3631 UMLS/C0085216 denotes ITC-
T8676 3317-3321 UMLS/C0806140 denotes Flow
T8675 3518-3525 UMLS/C1553451 denotes ystems,
T8674 3824-3829 UMLS/C1136359 denotes ells
T8673 3735-3740 UMLS/C1136359 denotes ells
T8672 3386-3391 UMLS/C1136359 denotes ells
T8671 3367-3373 UMLS/C0870078 denotes sample
T8670 3696-3711 UMLS/C0005574 denotes iotechnologies,
T8669 3354-3364 UMLS/C0185117 denotes expression
T8668 3599-3607 UMLS/C1719822 denotes ollowed
T8667 3780-3790 UMLS/C0301869 denotes onjugated
T8666 3632-3642 UMLS/C0301869 denotes onjugated
T8665 3729-3732 UMLS/C0041703 denotes SA)
T8664 3544-3547 UMLS/C0041703 denotes SA)
T8663 3884-3892 UMLS/C1524024 denotes nalysis
T8662 3333-3341 UMLS/C1524024 denotes analysis
T8661 3751-3760 UMLS/C1439852 denotes ncubated
T8660 3446-3455 UMLS/C1439852 denotes ncubated
T8659 3842-3846 UMLS/C0205170 denotes ell
T8658 3643-3647 UMLS/C1510458 denotes oat
T8657 3599-3607 UMLS/C0332283 denotes ollowed
T8656 3824-3829 UMLS/C1948049 denotes ells
T8655 3735-3740 UMLS/C1948049 denotes ells
T8654 3386-3391 UMLS/C1948049 denotes ells
T8653 3518-3525 UMLS/C0449913 denotes ystems,
T8652 3599-3607 UMLS/C0332282 denotes ollowed
T8651 3824-3829 UMLS/C0007634 denotes ells
T8650 3735-3740 UMLS/C0007634 denotes ells
T8649 3386-3391 UMLS/C0007634 denotes ells
T8648 3573-3580 UMLS/C1547100 denotes ontrol
T8647 3824-3829 UMLS/C1269647 denotes ells
T8646 3735-3740 UMLS/C1269647 denotes ells
T8645 3386-3391 UMLS/C1269647 denotes ells
T8644 3573-3580 UMLS/C0332298 denotes ontrol
T8643 3367-3373 UMLS/C0370003 denotes sample
T8642 3643-3647 UMLS/C1265549 denotes oat
T8641 3766-3779 UMLS/C0031789 denotes hycoerythrin-
T8640 3664-3683 UMLS/C0312586 denotes olyclonal antibody
T8639 3518-3525 UMLS/C1704459 denotes ystems,
T8638 3859-3862 UMLS/C1720830 denotes BS
T8637 3419-3422 UMLS/C1720830 denotes BS
T8636 3472-3486 UMLS/C0086045 denotes oncentrations
T8635 3871-3882 UMLS/C1979572 denotes ncubations.
T8634 3611-3621 UMLS/C1979572 denotes ncubation
T8633 3884-3892 UMLS/C0936012 denotes nalysis
T8632 3333-3341 UMLS/C0936012 denotes analysis
T8631 3643-3647 UMLS/C0018019 denotes oat
T8630 3659-3663 UMLS/C0020855 denotes gG1
T8629 3508-3512 UMLS/C0020855 denotes gG1;
T8628 3573-3580 UMLS/C0009932 denotes ontrol
T8627 3920-3934 UMLS/C0180279 denotes low cytometer
T8626 3897-3906 UMLS/C0884358 denotes erformed
T8625 3367-3373 UMLS/C0441621 denotes sample
T8624 3719-3723 UMLS/C0205203 denotes ruz,
T8623 3691-3695 UMLS/C0205203 denotes ruz
T8622 3573-3580 UMLS/C1550141 denotes ontrol
T8621 3573-3580 UMLS/C1882979 denotes ontrol
T8620 3436-3440 UMLS/C1321301 denotes BMC
T8619 3395-3399 UMLS/C1321301 denotes BMC
T8618 3472-3486 UMLS/C1446561 denotes oncentrations
T8617 3573-3580 UMLS/C0243148 denotes ontrol
T8679 3367-3373 UMLS/C2347026 denotes sample
T8678 3871-3882 UMLS/C1980044 denotes ncubations.
T8677 3611-3621 UMLS/C1980044 denotes ncubation
T7466 1735-1741 UMLS/C1442450 denotes 1 hour
T7465 1561-1573 UMLS/C0039401 denotes instructions
T7464 1881-1893 UMLS/C2348609 denotes upplemented
T7463 1667-1674 UMLS/C2347026 denotes samples
T7462 1610-1617 UMLS/C2347026 denotes samples
T7461 1561-1573 UMLS/C1442085 denotes instructions
T7460 1790-1812 UMLS/C1515100 denotes uspensions in culture
T7459 1546-1560 UMLS/C1546933 denotes manufacturer's
T7458 2157-2160 UMLS/C1552937 denotes n.
T7457 1905-1910 UMLS/C0019215 denotes EPES
T7456 2175-2180 UMLS/C1136359 denotes lls w
T7455 1785-1789 UMLS/C1136359 denotes ell
T7454 1667-1674 UMLS/C0870078 denotes samples
T7453 1610-1617 UMLS/C0870078 denotes samples
T7452 1969-1979 UMLS/C0030842 denotes enicillin,
T7451 1752-1757 UMLS/C0231290 denotes fter
T7450 1291-1304 UMLS/C0178913 denotes blood samples
T7449 1680-1690 UMLS/C0332255 denotes fragmented
T7448 1343-1350 UMLS/C0178587 denotes density
T7447 1593-1601 UMLS/C1764827 denotes isolated
T7446 1192-1201 UMLS/C0204727 denotes Isolation
T7445 1593-1601 UMLS/C1548221 denotes isolated
T7444 1805-1819 UMLS/C0010454 denotes ulture medium
T7443 2175-2180 UMLS/C1948049 denotes lls w
T7442 1785-1789 UMLS/C1948049 denotes ell
T7441 1831-1837 UMLS/C0439536 denotes edium;
T7440 1469-1475 UMLS/C0453946 denotes coated
T7439 2175-2180 UMLS/C0007634 denotes lls w
T7438 1785-1789 UMLS/C0007634 denotes ell
T7437 2157-2160 UMLS/C0439232 denotes n.
T7436 1633-1642 UMLS/C1552738 denotes described
T7435 2157-2160 UMLS/C0702093 denotes n.
T7434 1561-1573 UMLS/C0220931 denotes instructions
T7433 1561-1573 UMLS/C1292796 denotes instructions
T7432 1709-1720 UMLS/C0162745 denotes collagenase
T7431 1351-1360 UMLS/C0439182 denotes gradients
T7430 1546-1560 UMLS/C1705426 denotes manufacturer's
T7429 1695-1703 UMLS/C0012237 denotes digested
T7428 1667-1674 UMLS/C0370003 denotes samples
T7427 1610-1617 UMLS/C0370003 denotes samples
T7426 1767-1773 UMLS/C0040300 denotes issue
T7425 1469-1475 UMLS/C1704806 denotes coated
T7424 1476-1490 UMLS/C1328924 denotes magnetic beads
T7423 1308-1322 UMLS/C0007703 denotes centrifugation
T7422 1752-1757 UMLS/C0687676 denotes fter
T7421 1709-1720 UP/P08897 denotes collagenase
T7420 1529-1538 UMLS/C0680240 denotes according
T7419 1995-2007 UMLS/C0038425 denotes treptomycin;
T7418 1469-1475 UMLS/C1697272 denotes coated
T7417 1680-1690 UMLS/C1708096 denotes fragmented
T7416 2114-2120 UMLS/C0005971 denotes ates (
T7415 1469-1475 UMLS/C1705944 denotes coated
T7414 1969-1979 UMLS/C0220892 denotes enicillin,
T7413 1265-1273 UMLS/C1521827 denotes prepared
T7412 1709-1720 UP/P33437 denotes collagenase
T7411 2186-2195 UMLS/C1512335 denotes rvested a
T7410 1593-1601 UMLS/C1546680 denotes isolated
T7409 2218-2226 UMLS/C1998793 denotes rified b
T7408 1409-1417 UMLS/C1998793 denotes purified
T7407 1546-1560 UMLS/C0947322 denotes manufacturer's
T7406 1192-1201 UMLS/C0220862 denotes Isolation
T7405 1519-1526 UMLS/C0017480 denotes Germany
T7404 1881-1893 UMLS/C1549514 denotes upplemented
T7403 1423-1427 UMLS/C1321301 denotes PBMC
T7402 1254-1258 UMLS/C1321301 denotes PBMC
T7401 1218-1252 UMLS/C1321301 denotes Peripheral blood mononuclear cells
T7400 2064-2076 UMLS/C0039421 denotes echnologies)
T7399 2014-2026 UMLS/C0039421 denotes echnologies)
T7398 1844-1856 UMLS/C0039421 denotes echnologies,
T7397 1469-1475 UMLS/C1552080 denotes coated
T7396 1831-1837 UMLS/C0009458 denotes edium;
T7395 2059-2063 UMLS/C0376558 denotes ife
T7394 2009-2013 UMLS/C0376558 denotes ife
T7393 1839-1843 UMLS/C0376558 denotes ife
T7392 2175-2180 UMLS/C1704653 denotes lls w
T7391 1785-1789 UMLS/C1704653 denotes ell
T7390 1758-1766 UMLS/C0849355 denotes emoving
T7389 1933-1957 UMLS/C0311461 denotes onessential amino acids,
T7388 1658-1663 UMLS/C0443224 denotes fresh
T7387 1323-1327 UMLS/C0205136 denotes over
T7386 1382-1388 UMLS/C0038995 denotes Sweden
T7385 1546-1560 UMLS/C1548599 denotes manufacturer's
T7384 1593-1601 UMLS/C0370215 denotes isolated
T7383 1881-1893 UMLS/C1947943 denotes upplemented
T7382 2145-2148 UMLS/C0041703 denotes A)
T7381 1876-1879 UMLS/C0041703 denotes SA)
T7380 1680-1690 UMLS/C0486805 denotes fragmented
T7379 1192-1201 UMLS/C1608300 denotes Isolation
T7378 1291-1304 UMLS/C0005834 denotes blood samples
T7377 2083-2092 UMLS/C1439852 denotes ncubated
T7376 1192-1201 UMLS/C1608299 denotes Isolation
T7375 1192-1201 UMLS/C1548355 denotes Isolation
T7374 2175-2180 UMLS/C1269647 denotes lls w
T7373 1785-1789 UMLS/C1269647 denotes ell
T7372 1831-1837 UMLS/C1561547 denotes edium;
T7371 1725-1730 UMLS/C0011519 denotes DNase
T7370 1725-1730 UMLS/C0011522 denotes DNase
T7369 2114-2120 UMLS/C0407295 denotes ates (
T7368 1774-1780 UMLS/C0440266 denotes ebris,
T7367 1561-1573 UMLS/C0302828 denotes instructions
T7366 1593-1601 UMLS/C0205409 denotes isolated
T7365 1192-1201 UMLS/C0205409 denotes Isolation
T7364 1917-1928 UMLS/C0017797 denotes -glutamine,
T7363 1680-1690 UMLS/C0332472 denotes fragmented
T7362 1469-1475 UMLS/C1522408 denotes coated
T7361 1680-1690 UMLS/C0392767 denotes fragmented
T7360 1539-1541 UMLS/C0319022 denotes to
T7359 2114-2120 UMLS/C1139930 denotes ates (
T7358 1881-1893 UMLS/C0242295 denotes upplemented
T7357 1469-1475 UMLS/C0325028 denotes coated
T7356 1351-1360 UMLS/C0812409 denotes gradients
T7355 1192-1201 UMLS/C1547129 denotes Isolation
T7354 1695-1703 UMLS/C0868946 denotes digested
T7353 1767-1773 UMLS/C1547928 denotes issue
T7352 2230-2248 UMLS/C2347644 denotes sitive selection a
T7351 1431-1449 UMLS/C2347644 denotes positive selection
T7350 2205-2212 UMLS/C0039194 denotes cells w
T7349 1580-1587 UMLS/C0039194 denotes T cells
T7348 1396-1403 UMLS/C0039194 denotes T cells
T7347 1210-1217 UMLS/C0039194 denotes T cells
T7346 1667-1674 UMLS/C0441621 denotes samples
T7345 1610-1617 UMLS/C0441621 denotes samples
T7344 1469-1475 UMLS/C0999543 denotes coated
T7343 1959-1968 UMLS/C2346524 denotes 00 IU/ml
T7342 1831-1837 UMLS/C1705217 denotes edium;
T6855 1063-1069 UMLS/C1519269 denotes ontrol
T6854 486-490 UMLS/C0444504 denotes ± s
T6853 131-135 UMLS/C0444504 denotes mean
T6852 486-490 UMLS/C2348143 denotes ± s
T6851 131-135 UMLS/C2348143 denotes mean
T6850 354-366 UMLS/C0032950 denotes ednisolone (
T6849 1073-1081 UMLS/C2587213 denotes T sample
T6848 819-822 UMLS/C1413716 denotes l (
T6847 595-598 UMLS/C1413716 denotes eve
T6846 1086-1093 UMLS/C2347026 denotes e obtai
T6845 36-43 UMLS/C2347026 denotes samples
T6844 819-822 UMLS/C0006560 denotes l (
T6843 595-598 UMLS/C0006560 denotes eve
T6842 575-593 UMLS/C0006560 denotes tive protein (CRP)
T6841 183-192 UMLS/C0011900 denotes agnosed a
T6840 575-593 UP/P12245 denotes tive protein (CRP)
T6839 1033-1036 UMLS/C0338831 denotes 45.
T6838 126-129 UMLS/C0338831 denotes men
T6837 1086-1093 UMLS/C0870078 denotes e obtai
T6836 36-43 UMLS/C0870078 denotes samples
T6835 575-593 UP/O19062 denotes tive protein (CRP)
T6834 962-970 UMLS/C1555900 denotes emission
T6833 427-435 UMLS/C1555900 denotes ical par
T6832 563-567 UMLS/C0439227 denotes seru
T6831 307-318 UMLS/C1704771 denotes sociation)
T6830 48-72 UMLS/C2361270 denotes total patient population
T6829 307-318 UMLS/C1704770 denotes sociation)
T6828 436-446 UMLS/C1704769 denotes meters in
T6827 813-818 UMLS/C1151254 denotes P lev
T6826 569-574 UMLS/C1151254 denotes C-rea
T6825 477-484 UMLS/C0332283 denotes ows (me
T6824 696-703 UMLS/C0332849 denotes into tw
T6823 477-484 UMLS/C0332282 denotes ows (me
T6822 1056-1061 UMLS/C0439234 denotes ed as
T6821 176-181 UMLS/C0439234 denotes ars)
T6820 962-970 UMLS/C1611832 denotes emission
T6819 427-435 UMLS/C1611832 denotes ical par
T6818 1073-1081 UMLS/C0332298 denotes T sample
T6817 1170-1178 UMLS/C1705908 denotes ve infor
T6816 1122-1130 UMLS/C1705908 denotes dergoing
T6815 854-862 UMLS/C1705908 denotes ith inac
T6814 724-732 UMLS/C1705908 denotes with ac
T6813 682-690 UMLS/C1705908 denotes were di
T6812 460-468 UMLS/C1705908 denotes ents wer
T6811 330-338 UMLS/C1705908 denotes tients w
T6810 89-97 UMLS/C1705908 denotes patients
T6809 23-31 UMLS/C1705908 denotes Patients
T6808 713-719 UMLS/C1705429 denotes 24 pat
T6807 771-777 UMLS/C0234234 denotes nd/or
T6806 713-719 UMLS/C1705428 denotes 24 pat
T6805 804-812 UMLS/C0205250 denotes serum C
T6804 640-657 UP/P04207 denotes oid factor titer,
T6803 575-593 UP/P14847 denotes tive protein (CRP)
T6802 962-970 UMLS/C1608518 denotes emission
T6801 427-435 UMLS/C1608518 denotes ical par
T6800 575-593 UP/P49254 denotes tive protein (CRP)
T6799 819-822 UMLS/C1826658 denotes l (
T6798 595-598 UMLS/C1826658 denotes eve
T6797 193-202 UMLS/C0680240 denotes cording t
T6796 634-639 UMLS/C1547013 denotes heuma
T6795 634-639 UMLS/C1705943 denotes heuma
T6794 949-957 UMLS/C0243161 denotes or clini
T6793 223-231 UMLS/C0243161 denotes iteria o
T6792 575-593 UP/Q07203 denotes tive protein (CRP)
T6791 454-459 UMLS/C1705923 denotes y pat
T6790 296-306 UMLS/C0035435 denotes eumatism A
T6789 962-970 UMLS/C0205210 denotes emission
T6788 427-435 UMLS/C0205210 denotes ical par
T6787 913-920 UMLS/C0557806 denotes Rheuma
T6786 248-255 UMLS/C0557806 denotes llege o
T6785 924-936 UMLS/C0035452 denotes gy prelimina
T6784 259-271 UMLS/C0035452 denotes eumatology (
T6783 575-593 UP/P47875 denotes tive protein (CRP)
T6782 640-657 UMLS/C0035448 denotes oid factor titer,
T6781 713-719 UMLS/C0439745 denotes 24 pat
T6780 1073-1081 UMLS/C0243148 denotes T sample
T6779 1033-1036 UMLS/C1690528 denotes 45.
T6778 126-129 UMLS/C1690528 denotes men
T6777 904-912 UMLS/C0596070 denotes ollege o
T6776 287-295 UMLS/C0596070 denotes erican R
T6775 239-247 UMLS/C0596070 denotes erican C
T6774 575-593 UP/P02741 denotes tive protein (CRP)
T6773 819-822 UMLS/C1879974 denotes l (
T6772 595-598 UMLS/C1879974 denotes eve
T6771 575-593 UMLS/C1879974 denotes tive protein (CRP)
T6770 120-125 UMLS/C0205453 denotes seven
T6769 436-446 UMLS/C2350001 denotes meters in
T6768 436-446 UMLS/C0449381 denotes meters in
T6767 848-853 UMLS/C0205454 denotes ents
T6766 709-712 UMLS/C0205448 denotes ps:
T6765 813-818 UMLS/C1550100 denotes P lev
T6764 569-574 UMLS/C1550100 denotes C-rea
T6763 1113-1118 UMLS/C0205449 denotes tient
T6762 1028-1032 UMLS/C0205451 denotes age,
T6761 640-657 UP/P04206 denotes oid factor titer,
T6760 477-484 UMLS/C1719822 denotes ows (me
T6759 1170-1178 UMLS/C1550655 denotes ve infor
T6758 1122-1130 UMLS/C1550655 denotes dergoing
T6757 854-862 UMLS/C1550655 denotes ith inac
T6756 724-732 UMLS/C1550655 denotes with ac
T6755 682-690 UMLS/C1550655 denotes were di
T6754 460-468 UMLS/C1550655 denotes ents wer
T6753 330-338 UMLS/C1550655 denotes tients w
T6752 89-97 UMLS/C1550655 denotes patients
T6751 23-31 UMLS/C1550655 denotes Patients
T6750 713-719 UMLS/C1552839 denotes 24 pat
T6749 713-719 UMLS/C1257890 denotes 24 pat
T6748 1073-1081 UMLS/C1547100 denotes T sample
T6747 563-567 UMLS/C0564385 denotes seru
T6746 344-353 UMLS/C1514756 denotes ceiving p
T6745 515-545 UMLS/C1114891 denotes rocyte sedimentation rate, 55.
T6744 575-593 UP/P02742 denotes tive protein (CRP)
T6743 762-770 UMLS/C0439064 denotes tender
T6742 1179-1183 UMLS/C1442162 denotes ed c
T6741 515-545 UMLS/C0086250 denotes rocyte sedimentation rate, 55.
T6740 738-744 UMLS/C1553875 denotes isease
T6739 771-777 UMLS/C0402364 denotes nd/or
T6738 454-459 UMLS/C0557651 denotes y pat
T6737 1086-1093 UMLS/C0441621 denotes e obtai
T6736 36-43 UMLS/C0441621 denotes samples
T6735 971-980 UMLS/C0544452 denotes [26]. Six
T6734 868-884 UMLS/C0544452 denotes isease, who sati
T6733 937-948 UMLS/C0439611 denotes y criteria
T6732 1038-1041 UMLS/C1114365 denotes ± 1
T6731 158-161 UMLS/C1114365 denotes ge,
T6730 388-425 UMLS/C0242708 denotes ase-modifying antirheumatic drugs. Cl
T6729 1179-1183 UMLS/C1550718 denotes ed c
T6728 823-828 UMLS/C0456079 denotes 10 m
T6727 600-605 UMLS/C0456079 denotes 32.0
T6726 1073-1081 UMLS/C1550141 denotes T sample
T6725 486-490 UMLS/C1704970 denotes ± s
T6724 131-135 UMLS/C1704970 denotes mean
T6723 813-818 UMLS/C0229671 denotes P lev
T6722 569-574 UMLS/C0229671 denotes C-rea
T6721 210-217 UMLS/C1527075 denotes vised 1
T6720 813-818 UMLS/C1546774 denotes P lev
T6719 569-574 UMLS/C1546774 denotes C-rea
T6718 924-936 UMLS/C0334889 denotes gy prelimina
T6717 259-271 UMLS/C0334889 denotes eumatology (
T6716 823-828 UMLS/C0441889 denotes 10 m
T6715 600-605 UMLS/C0441889 denotes 32.0
T6714 1056-1061 UMLS/C0439508 denotes ed as
T6713 176-181 UMLS/C0439508 denotes ars)
T6712 1142-1164 UMLS/C0086511 denotes replacement. All patie
T6711 634-639 UMLS/C0456387 denotes heuma
T6710 1099-1107 UMLS/C1706701 denotes om three
T6709 1038-1041 UMLS/C0001811 denotes ± 1
T6708 158-161 UMLS/C0001811 denotes ge,
T6707 1038-1041 UMLS/C0001779 denotes ± 1
T6706 158-161 UMLS/C0001779 denotes ge,
T6705 713-719 UMLS/C0441833 denotes 24 pat
T6704 307-318 UMLS/C0699792 denotes sociation)
T6703 454-459 UMLS/C0008972 denotes y pat
T6702 890-899 UMLS/C0242428 denotes the Ameri
T6701 454-459 UMLS/C0008976 denotes y pat
T6700 1038-1041 UMLS/C0001792 denotes ± 1
T6699 158-161 UMLS/C0001792 denotes ge,
T6698 1099-1107 UMLS/C1301820 denotes om three
T6697 1086-1093 UMLS/C0370003 denotes e obtai
T6696 36-43 UMLS/C0370003 denotes samples
T6695 486-490 UMLS/C0876919 denotes ± s
T6694 131-135 UMLS/C0876919 denotes mean
T6693 1033-1036 UMLS/C1281583 denotes 45.
T6692 126-129 UMLS/C1281583 denotes men
T6691 376-382 UMLS/C0439423 denotes ay) an
T6690 1033-1036 UMLS/C0086418 denotes 45.
T6689 126-129 UMLS/C0086418 denotes men
T6688 376-382 UMLS/C0439422 denotes ay) an
T6687 1033-1036 UMLS/C0018563 denotes 45.
T6686 126-129 UMLS/C0018563 denotes men
T6685 713-719 UMLS/C1552516 denotes 24 pat
T6684 454-459 UMLS/C2603343 denotes y pat
T6683 1073-1081 UMLS/C0009932 denotes T sample
T6682 454-459 UMLS/C2349977 denotes y pat
T6681 1033-1036 UMLS/C0030736 denotes 45.
T6680 126-129 UMLS/C0030736 denotes men
T6679 1170-1178 UMLS/C0030705 denotes ve infor
T6678 1122-1130 UMLS/C0030705 denotes dergoing
T6677 854-862 UMLS/C0030705 denotes ith inac
T6676 724-732 UMLS/C0030705 denotes with ac
T6675 682-690 UMLS/C0030705 denotes were di
T6674 460-468 UMLS/C0030705 denotes ents wer
T6673 330-338 UMLS/C0030705 denotes tients w
T6672 89-97 UMLS/C0030705 denotes patients
T6671 23-31 UMLS/C0030705 denotes Patients
T6670 995-1013 UMLS/C1708335 denotes lunteers (11 women
T6669 819-822 UMLS/C0201657 denotes l (
T6668 595-598 UMLS/C0201657 denotes eve
T6667 575-593 UMLS/C0201657 denotes tive protein (CRP)
T6666 640-657 UMLS/C0201660 denotes oid factor titer,
T6665 307-318 UMLS/C0004083 denotes sociation)
T6664 1170-1178 UMLS/C1578479 denotes ve infor
T6663 1122-1130 UMLS/C1578479 denotes dergoing
T6662 854-862 UMLS/C1578479 denotes ith inac
T6661 724-732 UMLS/C1578479 denotes with ac
T6660 682-690 UMLS/C1578479 denotes were di
T6659 460-468 UMLS/C1578479 denotes ents wer
T6658 330-338 UMLS/C1578479 denotes tients w
T6657 89-97 UMLS/C1578479 denotes patients
T6656 23-31 UMLS/C1578479 denotes Patients
T6655 1170-1178 UMLS/C1578483 denotes ve infor
T6654 1122-1130 UMLS/C1578483 denotes dergoing
T6653 854-862 UMLS/C1578483 denotes ith inac
T6652 724-732 UMLS/C1578483 denotes with ac
T6651 682-690 UMLS/C1578483 denotes were di
T6650 460-468 UMLS/C1578483 denotes ents wer
T6649 330-338 UMLS/C1578483 denotes tients w
T6648 89-97 UMLS/C1578483 denotes patients
T6647 23-31 UMLS/C1578483 denotes Patients
T6646 1179-1183 UMLS/C1947971 denotes ed c
T6645 1056-1061 UMLS/C1561543 denotes ed as
T6644 176-181 UMLS/C1561543 denotes ars)
T6643 1170-1178 UMLS/C1578480 denotes ve infor
T6642 1122-1130 UMLS/C1578480 denotes dergoing
T6641 854-862 UMLS/C1578480 denotes ith inac
T6640 724-732 UMLS/C1578480 denotes with ac
T6639 682-690 UMLS/C1578480 denotes were di
T6638 460-468 UMLS/C1578480 denotes ents wer
T6637 330-338 UMLS/C1578480 denotes tients w
T6636 89-97 UMLS/C1578480 denotes patients
T6635 23-31 UMLS/C1578480 denotes Patients
T6634 1170-1178 UMLS/C1578481 denotes ve infor
T6633 1122-1130 UMLS/C1578481 denotes dergoing
T6632 854-862 UMLS/C1578481 denotes ith inac
T6631 724-732 UMLS/C1578481 denotes with ac
T6630 682-690 UMLS/C1578481 denotes were di
T6629 460-468 UMLS/C1578481 denotes ents wer
T6628 330-338 UMLS/C1578481 denotes tients w
T6627 89-97 UMLS/C1578481 denotes patients
T6626 23-31 UMLS/C1578481 denotes Patients
T6625 1170-1178 UMLS/C1578486 denotes ve infor
T6624 1122-1130 UMLS/C1578486 denotes dergoing
T6623 854-862 UMLS/C1578486 denotes ith inac
T6622 724-732 UMLS/C1578486 denotes with ac
T6621 682-690 UMLS/C1578486 denotes were di
T6620 460-468 UMLS/C1578486 denotes ents wer
T6619 330-338 UMLS/C1578486 denotes tients w
T6618 89-97 UMLS/C1578486 denotes patients
T6617 23-31 UMLS/C1578486 denotes Patients
T6616 1170-1178 UMLS/C1578484 denotes ve infor
T6615 1122-1130 UMLS/C1578484 denotes dergoing
T6614 854-862 UMLS/C1578484 denotes ith inac
T6613 724-732 UMLS/C1578484 denotes with ac
T6612 682-690 UMLS/C1578484 denotes were di
T6611 460-468 UMLS/C1578484 denotes ents wer
T6610 330-338 UMLS/C1578484 denotes tients w
T6609 89-97 UMLS/C1578484 denotes patients
T6608 23-31 UMLS/C1578484 denotes Patients
T6607 1170-1178 UMLS/C1578485 denotes ve infor
T6606 1122-1130 UMLS/C1578485 denotes dergoing
T6605 854-862 UMLS/C1578485 denotes ith inac
T6604 724-732 UMLS/C1578485 denotes with ac
T6603 682-690 UMLS/C1578485 denotes were di
T6602 460-468 UMLS/C1578485 denotes ents wer
T6601 330-338 UMLS/C1578485 denotes tients w
T6600 89-97 UMLS/C1578485 denotes patients
T6599 23-31 UMLS/C1578485 denotes Patients
T6598 745-752 UMLS/C0012634 denotes who ha
T6597 344-353 UMLS/C1709850 denotes ceiving p
T6596 738-744 UMLS/C1561511 denotes isease
T6595 738-744 UMLS/C1561512 denotes isease
T6594 738-744 UMLS/C1561513 denotes isease
T6593 307-318 UMLS/C0596306 denotes sociation)
T6592 1033-1036 UMLS/C0949399 denotes 45.
T6591 126-129 UMLS/C0949399 denotes men
T6590 1028-1032 UMLS/C1533153 denotes age,
T6589 307-318 UMLS/C0439849 denotes sociation)
T6588 575-593 UP/P19094 denotes tive protein (CRP)
T6587 454-459 UMLS/C1880229 denotes y pat
T6586 738-744 UMLS/C0205177 denotes isease
T6585 713-719 UMLS/C1561557 denotes 24 pat
T6584 1056-1061 UMLS/C1561544 denotes ed as
T6583 176-181 UMLS/C1561544 denotes ars)
T6582 103-109 UMLS/C0767847 denotes RA (25
T6581 785-799 UMLS/C0152031 denotes joints and ele
T6580 819-822 UMLS/C1413766 denotes l (
T6579 595-598 UMLS/C1413766 denotes eve
T6578 515-545 UMLS/C1176468 denotes rocyte sedimentation rate, 55.
T6577 738-744 UMLS/C2347179 denotes isease
T6576 203-205 UMLS/C0319022 denotes t
T6575 713-719 UMLS/C1522242 denotes 24 pat
T6574 630-633 UMLS/C0020861 denotes ss
T6573 515-545 UMLS/C1619634 denotes rocyte sedimentation rate, 55.
T6572 738-744 UMLS/C1561510 denotes isease
T6571 738-744 UMLS/C1561509 denotes isease
T6570 738-744 UMLS/C1561508 denotes isease
T6569 738-744 UMLS/C1561507 denotes isease
T6568 494-512 UMLS/C0871420 denotes ard deviation): er
T6567 139-157 UMLS/C0871420 denotes tandard deviation
T6566 436-446 UMLS/C0549193 denotes meters in
T6565 454-459 UMLS/C0947630 denotes y pat
T6564 658-663 UMLS/C0475208 denotes 142 ±
T6563 634-639 UMLS/C0489477 denotes heuma
T6562 486-490 UMLS/C2347634 denotes ± s
T6561 131-135 UMLS/C2347634 denotes mean
T6560 575-593 UP/P49262 denotes tive protein (CRP)
T6559 738-744 UMLS/C1547419 denotes isease
T6558 1073-1081 UMLS/C1882979 denotes T sample

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T12021 7963-7974 JJ denotes significant
T12020 7952-7962 VB denotes considered
T12019 7948-7951 VB denotes was
T12018 7943-7947 CD denotes 0.05
T12017 7941-7942 SYM denotes <
T12016 7939-7940 NN denotes P
T12015 7933-7937 NN denotes test
T12014 7928-7932 NN denotes rank
T12013 7921-7927 VB denotes signed
T12012 7912-7920 NNP denotes Wilcoxon
T12011 7908-7911 DT denotes the
T12010 7905-7907 CC denotes or
T12009 7900-7904 NN denotes test
T12008 7898-7899 NNP denotes U
T12007 7885-7897 NNP denotes Mann–Whitney
T12006 7881-7884 DT denotes the
T12005 7878-7880 IN denotes by
T12004 7867-7877 VB denotes determined
T12003 7863-7866 VB denotes was
T12002 7856-7862 NN denotes groups
T12001 7852-7855 CD denotes two
T12000 7844-7851 IN denotes between
T11999 7832-7843 NN denotes differences
T11998 7829-7831 IN denotes of
T11997 7816-7828 NN denotes significance
T11996 7804-7815 JJ denotes statistical
T11995 7800-7803 DT denotes The
T11994 7793-7798 NN denotes plots
T11993 7789-7792 NN denotes box
T11992 7786-7788 CC denotes or
T11991 7781-7785 NN denotes mean
T11990 7777-7780 DT denotes the
T11989 7774-7776 IN denotes of
T11988 7768-7773 NN denotes error
T11987 7759-7767 JJ denotes standard
T11986 7757-7758 -COLON- denotes ±
T11985 7751-7756 NN denotes value
T11984 7746-7750 JJ denotes mean
T11983 7742-7745 DT denotes the
T11982 7739-7741 IN denotes as
T11981 7729-7738 VB denotes expressed
T11980 7725-7728 VB denotes are
T11979 7720-7724 NN denotes Data
T11978 7711-7719 NN denotes analysis
T11977 7699-7710 JJ denotes Statistical
T11404 7680-7696 NN denotes phosophorylation
T11403 7674-7679 NN denotes STAT1
T11402 7670-7673 IN denotes for
T11401 7662-7669 NN denotes control
T11400 7653-7661 JJ denotes positive
T11399 7651-7652 DT denotes a
T11398 7648-7650 IN denotes as
T11397 7643-7647 VB denotes used
T11396 7638-7642 VB denotes were
T11395 7632-7637 NN denotes cells
T11394 7627-7631 NN denotes Hela
T11393 7610-7626 JJ denotes IFN-γ-stimulated
T11392 7601-7608 NN denotes protein
T11391 7596-7600 NN denotes STAT
T11390 7593-7595 IN denotes of
T11389 7586-7592 NN denotes amount
T11315 7117-7125 NN denotes tyrosine
T11314 7097-7116 JJ denotes anti-phosphorylated
T11313 7093-7096 CC denotes and
T11312 7091-7092 -COMMA- denotes ,
T11311 7083-7091 NN denotes antibody
T11310 7072-7082 JJ denotes anti-STAT3
T11309 7070-7071 -COMMA- denotes ,
T11308 7062-7070 NN denotes antibody
T11307 7056-7061 NN denotes STAT1
T11306 7053-7055 IN denotes of
T11305 7049-7052 CD denotes 701
T11304 7040-7048 NN denotes tyrosine
T11303 7020-7039 JJ denotes anti-phosphorylated
T11302 7018-7019 -COMMA- denotes ,
T11301 7010-7018 NN denotes antibody
T11300 6999-7009 JJ denotes anti-STAT1
T11299 6997-6998 -RRB- denotes )
T11298 6994-6997 NN denotes IgG
T11297 6987-6993 NN denotes rabbit
T11296 6986-6987 -LRB- denotes (
T11295 6975-6985 NN denotes antibodies
T11294 6971-6974 DT denotes the
T11293 6966-6970 IN denotes with
T11292 6956-6965 VB denotes incubated
T11291 6952-6955 VB denotes was
T11290 6943-6951 NN denotes membrane
T11289 6939-6942 DT denotes the
T11288 6937-6938 -COMMA- denotes ,
T11287 6930-6937 NNP denotes Briefly
T11286 6916-6928 NN denotes instructions
T11285 6913-6915 POS denotes 's
T11284 6901-6913 NN denotes manufacturer
T11283 6897-6900 DT denotes the
T11282 6894-6896 TO denotes to
T11281 6884-6893 VB denotes according
T11280 6882-6883 -RRB- denotes )
T11279 6879-6882 NNP denotes USA
T11278 6877-6878 -COMMA- denotes ,
T11277 6875-6877 NNP denotes MA
T11276 6873-6874 -COMMA- denotes ,
T11275 6866-6873 NNP denotes Beverly
T11274 6864-6865 -COMMA- denotes ,
T11273 6854-6864 NNP denotes Technology
T11272 6844-6853 NNP denotes Signaling
T11271 6839-6843 NNP denotes Cell
T11270 6838-6839 -LRB- denotes (
T11269 6833-6837 NN denotes kits
T11268 6823-6832 JJ denotes available
T11267 6812-6822 JJ denotes commercial
T11266 6806-6811 VB denotes using
T11265 6797-6805 VB denotes detected
T11264 6793-6796 VB denotes was
T11263 6787-6792 NN denotes STAT3
T11262 6783-6786 CC denotes and
T11261 6777-6782 NN denotes STAT1
T11260 6774-6776 IN denotes of
T11259 6758-6773 NN denotes phosphorylation
T11258 6749-6757 NN denotes Tyrosine
T11257 6745-6747 CD denotes 20
T11256 6739-6744 CD denotes Tween
T11255 6737-6738 NN denotes %
T11254 6734-6737 CD denotes 0.1
T11253 6729-6733 IN denotes with
T11252 6722-6728 NN denotes saline
T11251 6708-6721 JJ denotes Tris-buffered
T11250 6705-6707 IN denotes in
T11249 6700-6704 NN denotes milk
T11248 6695-6699 NN denotes skim
T11247 6693-6694 NN denotes %
T11246 6692-6693 CD denotes 5
T11245 6687-6691 IN denotes with
T11244 6679-6686 VB denotes blocked
T11243 6675-6678 VB denotes was
T11242 6666-6674 NN denotes membrane
T11241 6662-6665 DT denotes the
T11240 6658-6661 CC denotes and
T11239 6656-6657 -COMMA- denotes ,
T11238 6655-6656 -RRB- denotes )
T11237 6653-6655 NNP denotes UK
T11236 6651-6652 -COMMA- denotes ,
T11235 6636-6651 NNP denotes Buckinghamshire
T11234 6634-6635 -COMMA- denotes ,
T11233 6626-6634 NNP denotes Amersham
T11232 6625-6626 -LRB- denotes (
T11231 6615-6624 NN denotes membranes
T11230 6600-6614 VB denotes nitrocellulose
T11229 6597-6599 TO denotes to
T11228 6585-6596 VB denotes transferred
T11227 6580-6584 VB denotes were
T11226 6576-6579 CC denotes and
T11225 6571-6575 NN denotes gels
T11224 6552-6570 NN denotes SDS-polyacrylamide
T11223 6550-6551 NN denotes %
T11222 6548-6550 CD denotes 10
T11221 6545-6547 IN denotes on
T11220 6532-6544 VB denotes fractionated
T11219 6527-6531 VB denotes were
T11218 6519-6526 NN denotes samples
T11217 6511-6518 NN denotes protein
T11216 6508-6510 NN denotes μl
T11215 6505-6507 CD denotes 20
T11214 6500-6504 RB denotes Then
T11213 6497-6498 -RRB- denotes )
T11212 6493-6497 NN denotes blue
T11211 6482-6492 NN denotes bromphenol
T11210 6480-6481 NN denotes %
T11209 6477-6480 CD denotes 0.1
T11208 6475-6476 -COMMA- denotes ,
T11207 6461-6475 NN denotes dithiothreitol
T11206 6458-6460 NN denotes mM
T11205 6455-6457 CD denotes 50
T11204 6453-6454 -COMMA- denotes ,
T11203 6445-6453 NN denotes glycerol
T11202 6443-6444 NN denotes %
T11201 6441-6443 CD denotes 10
T11200 6439-6440 -COMMA- denotes ,
T11199 6436-6439 NN denotes SDS
T11198 6434-6435 NN denotes %
T11197 6433-6434 CD denotes 2
T11196 6431-6432 -COMMA- denotes ,
T11195 6430-6431 -RRB- denotes ]
T11194 6427-6430 CD denotes 6.8
T11193 6424-6426 NN denotes pH
T11192 6423-6424 -LRB- denotes [
T11191 6414-6422 NN denotes Tris–HCl
T11190 6411-6413 NN denotes mM
T11189 6406-6410 CD denotes 62.5
T11188 6405-6406 -LRB- denotes (
T11187 6398-6404 NN denotes buffer
T11186 6391-6397 VB denotes lysing
T11185 6387-6390 NN denotes SDS
T11184 6384-6386 NN denotes μl
T11183 6380-6383 CD denotes 100
T11182 6377-6379 IN denotes in
T11181 6371-6376 NN denotes cells
T11180 6363-6370 VB denotes placing
T11179 6360-6362 IN denotes by
T11178 6351-6359 VB denotes prepared
T11177 6346-6350 VB denotes were
T11176 6338-6345 NN denotes lysates
T11175 6333-6337 NN denotes cell
T11174 6327-6332 JJ denotes Whole
T11173 6324-6325 -RRB- denotes )
T11172 6318-6324 NN denotes Techne
T11171 6317-6318 -LRB- denotes (
T11170 6313-6316 NN denotes IgG
T11169 6308-6312 NN denotes goat
T11168 6300-6307 NN denotes control
T11167 6297-6299 CC denotes or
T11166 6295-6296 -RRB- denotes )
T11165 6292-6295 NNP denotes USA
T11164 6290-6291 -COMMA- denotes ,
T11163 6288-6290 NNP denotes NJ
T11162 6286-6287 -COMMA- denotes ,
T11161 6277-6286 NNP denotes Princeton
T11160 6275-6276 -COMMA- denotes ,
T11159 6269-6275 NNP denotes Techne
T11158 6267-6268 -COLON- denotes ;
T11157 6264-6267 NN denotes IgG
T11156 6263-6264 -LRB- denotes (
T11155 6254-6262 NN denotes antibody
T11154 6243-6253 JJ denotes polyclonal
T11153 6233-6242 JJ denotes anti-IL-6
T11152 6228-6232 NN denotes goat
T11151 6215-6227 VB denotes neutralizing
T11150 6209-6214 NN denotes μg/ml
T11149 6206-6208 CD denotes 40
T11148 6201-6205 IN denotes with
T11147 6194-6200 NN denotes medium
T11146 6186-6193 NN denotes culture
T11145 6183-6185 IN denotes in
T11144 6177-6182 NN denotes serum
T11143 6174-6176 NN denotes RA
T11142 6167-6173 JJ denotes active
T11141 6165-6166 NN denotes %
T11140 6163-6165 CD denotes 30
T11139 6158-6162 IN denotes with
T11138 6154-6157 NN denotes min
T11137 6151-6153 CD denotes 20
T11136 6147-6150 IN denotes for
T11135 6136-6146 VB denotes stimulated
T11134 6131-6135 VB denotes were
T11133 6125-6130 NN denotes cells
T11132 6123-6124 NN denotes T
T11131 6118-6122 JJ denotes CD4+
T11130 6111-6117 JJ denotes normal
T11129 6109-6110 -COMMA- denotes ,
T11128 6094-6109 NN denotes phosphorylation
T11127 6089-6093 NN denotes STAT
T11126 6086-6088 IN denotes on
T11125 6081-6085 NN denotes IL-6
T11124 6075-6080 NN denotes serum
T11123 6072-6074 IN denotes of
T11122 6065-6071 NN denotes effect
T11121 6061-6064 DT denotes the
T11120 6053-6060 VB denotes examine
T11119 6050-6052 TO denotes To
T11118 6045-6048 NN denotes FCS
T11117 6043-6044 NN denotes %
T11116 6041-6043 CD denotes 10
T11115 6036-6040 IN denotes with
T11114 6029-6035 NN denotes medium
T11113 6021-6028 NN denotes culture
T11112 6018-6020 NN denotes ml
T11111 6014-6017 CD denotes 0.5
T11110 6011-6013 IN denotes in
T11109 6005-6010 NN denotes cells
T11108 6001-6004 CD denotes 105
T11107 5999-6000 IN denotes ×
T11106 5997-5998 CD denotes 5
T11105 5994-5996 IN denotes of
T11104 5986-5993 NN denotes density
T11103 5984-5985 DT denotes a
T11102 5981-5983 IN denotes at
T11101 5976-5980 NN denotes IL-6
T11100 5972-5975 CC denotes and
T11096 5938-5947 VB denotes indicated
T11095 5934-5937 DT denotes the
T11094 5931-5933 IN denotes by
T11093 5927-5930 NN denotes min
T11092 5924-5926 CD denotes 20
T11091 5920-5923 IN denotes for
T11090 5909-5919 VB denotes stimulated
T11089 5904-5908 VB denotes were
T11088 5898-5903 NN denotes cells
T11087 5896-5897 NN denotes T
T11086 5891-5895 JJ denotes CD4+
T11085 5882-5890 NN denotes analysis
T11084 5877-5881 NN denotes blot
T11083 5869-5876 NN denotes Western
T11388 7580-7585 JJ denotes total
T11387 7576-7579 DT denotes the
T11386 7571-7575 IN denotes with
T11385 7562-7570 VB denotes compared
T11384 7558-7561 VB denotes was
T11383 7542-7557 NN denotes phosphorylation
T11382 7537-7541 NN denotes STAT
T11381 7533-7536 CC denotes and
T11380 7531-7532 -COMMA- denotes ,
T11379 7523-7531 NN denotes analysis
T11378 7513-7522 NN denotes NIH-Image
T11377 7507-7512 VB denotes using
T11376 7494-7506 NN denotes densitometry
T11375 7491-7493 IN denotes by
T11374 7480-7490 VB denotes quantified
T11373 7475-7479 VB denotes were
T11372 7469-7474 NN denotes bands
T11371 7461-7468 NN denotes Protein
T11370 7453-7459 NN denotes system
T11369 7435-7452 NN denotes chemiluminescence
T11368 7431-7434 DT denotes the
T11367 7425-7430 VB denotes using
T11366 7416-7424 VB denotes revealed
T11365 7412-7415 VB denotes was
T11364 7408-7411 CC denotes and
T11363 7406-7407 -COMMA- denotes ,
T11362 7403-7406 NN denotes BSA
T11361 7401-7402 NN denotes %
T11360 7400-7401 CD denotes 5
T11359 7395-7399 IN denotes with
T11358 7392-7394 CD denotes 20
T11357 7386-7391 CD denotes Tween
T11356 7384-7385 NN denotes %
T11355 7381-7384 CD denotes 0.1
T11354 7376-7380 IN denotes with
T11353 7369-7375 NN denotes saline
T11352 7355-7368 JJ denotes Tris-buffered
T11351 7350-7354 IN denotes with
T11350 7343-7349 CD denotes 1/4000
T11349 7340-7342 IN denotes at
T11348 7332-7339 VB denotes diluted
T11347 7323-7331 NN denotes antibody
T11346 7319-7322 NN denotes IgG
T11345 7307-7318 JJ denotes anti-rabbit
T11344 7285-7306 JJ denotes peroxidase-conjugated
T11343 7273-7284 JJ denotes horseradish
T11342 7270-7272 IN denotes by
T11341 7261-7269 VB denotes detected
T11340 7257-7260 VB denotes was
T11339 7249-7256 NN denotes binding
T11338 7240-7248 NN denotes Antibody
T11337 7235-7238 NN denotes BSA
T11336 7233-7234 NN denotes %
T11335 7232-7233 CD denotes 5
T11334 7227-7231 IN denotes with
T11333 7224-7226 CD denotes 20
T11332 7218-7223 CD denotes Tween
T11331 7216-7217 NN denotes %
T11330 7213-7216 CD denotes 0.1
T11329 7208-7212 IN denotes with
T11328 7201-7207 NN denotes saline
T11327 7187-7200 JJ denotes Tris-buffered
T11326 7182-7186 IN denotes with
T11325 7175-7181 CD denotes 1/2000
T11324 7172-7174 IN denotes at
T11323 7160-7171 VB denotes recommended
T11322 7157-7159 IN denotes as
T11321 7149-7156 VB denotes diluted
T11320 7147-7148 -COMMA- denotes ,
T11319 7139-7147 NN denotes antibody
T11318 7133-7138 NN denotes STAT3
T11317 7130-7132 IN denotes of
T11316 7126-7129 CD denotes 705
T11099 5966-5971 NN denotes IL-10
T11098 5963-5965 IN denotes of
T11097 5948-5962 NN denotes concentrations
T10112 5865-5866 -DQE- denotes '
T10111 5842-5865 NN denotes 5'-AGGGGCCGGCTCAACACC-3
T10110 5838-5841 CC denotes and
T10109 5836-5837 -DQE- denotes '
T10108 5813-5836 NN denotes 5'-CCCGCCGGCACCTTTCTG-3
T10107 5811-5812 -COMMA- denotes ,
T10106 5806-5811 NN denotes SOCS3
T10105 5802-5805 IN denotes for
T10104 5798-5801 CC denotes and
T10103 5796-5797 -COLON- denotes ;
T10102 5794-5795 -DQE- denotes '
T10101 5769-5794 NN denotes 5'-GCACAGCAGAAAAATAAAGC-3
T10100 5765-5768 CC denotes and
T10099 5763-5764 -DQE- denotes '
T10098 5738-5763 NN denotes 5'-AGACCCCTTCTCACCTCTTG-3
T10097 5736-5737 -COMMA- denotes ,
T10096 5731-5736 NN denotes SOCS1
T10095 5727-5730 IN denotes for
T10094 5725-5726 -COLON- denotes ;
T10093 5723-5724 -DQE- denotes '
T10092 5694-5723 NN denotes 5'-CTCCTTAATGTCACGCACGATTTC-3
T10091 5690-5693 CC denotes and
T10090 5688-5689 -DQE- denotes '
T10089 5663-5688 NN denotes 5'-GTGGGGCGCCCCAGGCACCA-3
T10088 5661-5662 -COMMA- denotes ,
T10087 5654-5661 NN denotes β-actin
T10086 5650-5653 IN denotes for
T10085 5648-5649 -COLON- denotes :
T10084 5641-5648 VB denotes follows
T10083 5638-5640 IN denotes as
T10082 5633-5637 VB denotes were
T10081 5625-5632 NN denotes primers
T10080 5617-5624 JJ denotes reverse
T10079 5613-5616 CC denotes and
T10078 5605-5612 JJ denotes forward
T10077 5601-5604 DT denotes The
T10076 5589-5599 NN denotes expression
T10075 5581-5588 JJ denotes β-actin
T10074 5578-5580 TO denotes to
T10073 5569-5577 JJ denotes relative
T10072 5557-5568 VB denotes normalizing
T10071 5554-5556 IN denotes by
T10070 5543-5553 VB denotes determined
T10069 5538-5542 VB denotes were
T10068 5527-5537 NN denotes expression
T10067 5521-5526 NN denotes SOCS3
T10066 5517-5520 CC denotes and
T10065 5511-5516 NN denotes SOCS1
T10064 5508-5510 IN denotes of
T10063 5501-5507 NN denotes levels
T10062 5497-5500 DT denotes The
T10061 5486-5495 VB denotes performed
T10060 5481-5485 RB denotes then
T10059 5477-5480 VB denotes was
T10058 5468-5476 NN denotes analysis
T10057 5462-5467 NN denotes curve
T10056 5454-5461 NN denotes melting
T10055 5452-5453 DT denotes a
T10054 5448-5451 CC denotes and
T10053 5446-5447 -COMMA- denotes ,
T10052 5438-5446 VB denotes obtained
T10051 5433-5437 VB denotes were
T10050 5426-5432 NN denotes number
T10049 5420-5425 NN denotes cycle
T10048 5413-5419 CC denotes versus
T10047 5406-5412 NN denotes values
T10046 5393-5405 NN denotes fluorescence
T10045 5389-5392 DT denotes the
T10044 5386-5388 IN denotes of
T10043 5379-5385 NN denotes curves
T10042 5365-5378 NN denotes Amplification
T10041 5358-5363 NN denotes SOCS3
T10040 5354-5357 IN denotes for
T10039 5352-5353 -RRB- denotes )
T10038 5351-5352 NN denotes s
T10037 5348-5350 CD denotes 15
T10036 5347-5348 -LRB- denotes (
T10035 5345-5346 NN denotes C
T10034 5341-5344 CD denotes 72°
T10033 5338-5340 IN denotes at
T10032 5334-5337 CC denotes and
T10031 5332-5333 -COMMA- denotes ,
T10030 5331-5332 -RRB- denotes )
T10029 5330-5331 NN denotes s
T10028 5327-5329 CD denotes 15
T10027 5326-5327 -LRB- denotes (
T10026 5324-5325 NN denotes C
T10025 5320-5323 CD denotes 68°
T10024 5317-5319 IN denotes at
T10023 5315-5316 -COMMA- denotes ,
T10022 5314-5315 -RRB- denotes )
T10021 5313-5314 NN denotes s
T10020 5310-5312 CD denotes 10
T10019 5309-5310 -LRB- denotes (
T10018 5307-5308 NN denotes C
T10017 5303-5306 CD denotes 96°
T10016 5300-5302 IN denotes at
T10015 5296-5299 CC denotes and
T10014 5294-5295 -COLON- denotes ;
T10013 5289-5294 NN denotes SOCS1
T9961 5165-5166 -COLON- denotes :
T9960 5159-5165 NN denotes cycles
T9959 5153-5158 CD denotes 40–50
T9958 5149-5152 IN denotes for
T9957 5139-5148 VB denotes amplified
T9956 5134-5138 RB denotes then
T9955 5129-5133 VB denotes were
T9954 5125-5128 CC denotes and
T9953 5123-5124 -COMMA- denotes ,
T9952 5120-5123 NN denotes min
T9951 5117-5119 CD denotes 10
T9950 5113-5116 IN denotes for
T9949 5111-5112 NN denotes C
T9948 5107-5110 CD denotes 95°
T9947 5104-5106 IN denotes at
T9946 5094-5103 VB denotes denatured
T9945 5089-5093 VB denotes were
T9944 5081-5088 NN denotes samples
T9943 5076-5080 NN denotes cDNA
T9942 5072-5075 DT denotes The
T9941 5061-5070 NN denotes dilutions
T9940 5056-5060 NN denotes cDNA
T9939 5053-5055 TO denotes to
T9938 5047-5052 VB denotes added
T9937 5042-5046 VB denotes were
T9936 5034-5041 NN denotes primers
T9935 5025-5033 JJ denotes specific
T9934 5021-5024 CC denotes and
T9933 5019-5020 -RRB- denotes )
T9932 5008-5019 NNP denotes Diagnostics
T9931 5002-5007 NNP denotes Roche
T9930 5000-5001 -COLON- denotes ;
T9929 4999-5000 NNP denotes I
T9928 4993-4998 NNP denotes Green
T9927 4988-4992 NNP denotes SYBR
T9926 4981-4987 NNP denotes Master
T9925 4977-4980 NN denotes DNA
T9924 4967-4976 NNP denotes FastStart
T9923 4955-4966 NNP denotes Lightcycler
T9922 4954-4955 -LRB- denotes (
T9921 4950-4953 NN denotes dye
T9920 4948-4949 CD denotes I
T9919 4942-4947 NNP denotes Green
T9918 4937-4941 NN denotes SYBR
T9917 4914-4936 JJ denotes double-strand-specific
T9916 4910-4913 NN denotes DNA
T9915 4906-4909 CC denotes and
T9914 4895-4905 NN denotes polymerase
T9913 4891-4894 NN denotes DNA
T9912 4887-4890 NN denotes Taq
T9911 4876-4886 VB denotes containing
T9910 4872-4875 NN denotes mix
T9909 4863-4871 NN denotes reaction
T9908 4859-4862 DT denotes The
T9907 4846-4857 NN denotes capillaries
T9906 4840-4845 NN denotes glass
T9905 4837-4839 IN denotes in
T9904 4835-4836 -RRB- denotes )
T9903 4828-4835 NNP denotes Germany
T9902 4826-4827 -COMMA- denotes ,
T9901 4818-4826 NNP denotes Penzberg
T9900 4816-4817 -COMMA- denotes ,
T9899 4805-4816 NNP denotes Diagnostics
T9898 4799-4804 NNP denotes Roche
T9897 4798-4799 -LRB- denotes (
T9896 4787-4797 NNP denotes Instrument
T9895 4775-4786 NNP denotes LightCycler
T9894 4771-4774 DT denotes the
T9893 4766-4770 IN denotes with
T9892 4756-4765 VB denotes performed
T9891 4752-4755 VB denotes was
T9890 4748-4751 NN denotes PCR
T9889 4738-4747 JJ denotes Real-time
T9888 4735-4736 -RRB- denotes )
T9887 4732-4735 NNP denotes USA
T9886 4730-4731 -COMMA- denotes ,
T9885 4728-4730 NNP denotes WI
T9884 4726-4727 -COMMA- denotes ,
T9883 4719-4726 NNP denotes Madison
T9882 4717-4718 -COMMA- denotes ,
T9881 4710-4717 NNP denotes Promega
T9880 4709-4710 -LRB- denotes (
T9879 4701-4708 NN denotes primers
T9878 4698-4700 CD denotes 15
T9877 4697-4698 -RRB- denotes )
T9876 4695-4697 NN denotes dT
T9875 4694-4695 -LRB- denotes (
T9874 4688-4694 NN denotes oligo-
T9873 4684-4687 CC denotes and
T9872 4682-4683 -RRB- denotes )
T9871 4679-4682 NNP denotes USA
T9870 4677-4678 -COMMA- denotes ,
T9869 4675-4677 NNP denotes OH
T9868 4673-4674 -COMMA- denotes ,
T9867 4664-4673 NNP denotes Cleveland
T9866 4662-4663 -COMMA- denotes ,
T9865 4651-4662 NNP denotes Biochemical
T9864 4648-4650 NNP denotes US
T9863 4647-4648 -LRB- denotes (
T9862 4633-4646 NN denotes transcriptase
T9861 4625-4632 JJ denotes reverse
T9860 4619-4624 NN denotes virus
T9859 4610-4618 NN denotes leukemia
T9858 4603-4609 JJ denotes murine
T9857 4596-4602 NNP denotes Molony
T9856 4591-4595 IN denotes with
T9855 4587-4590 NN denotes RNA
T9854 4581-4586 JJ denotes total
T9853 4576-4580 IN denotes from
T9852 4564-4575 VB denotes synthesized
T9851 4560-4563 VB denotes was
T9850 4555-4559 NN denotes cDNA
T9849 4541-4554 NN denotes instructions.
T9848 4538-4540 POS denotes 's
T9847 4526-4538 NN denotes manufacturer
T9846 4522-4525 DT denotes the
T9845 4519-4521 TO denotes to
T9844 4509-4518 VB denotes according
T9843 4507-4508 -COMMA- denotes ,
T9842 4506-4507 -RRB- denotes )
T9841 4503-4506 NNP denotes USA
T9840 4501-4502 -COMMA- denotes ,
T9839 4499-4501 NNP denotes CA
T9838 4497-4498 -COMMA- denotes ,
T9837 4489-4497 NNP denotes Valencia
T9836 4487-4488 -COMMA- denotes ,
T9835 4481-4487 NNP denotes Qiagen
T9834 4479-4480 -COLON- denotes ;
T9833 4476-4479 NN denotes kit
T9832 4471-4475 NNP denotes Mini
T9831 4464-4470 NNP denotes RNeasy
T9830 4463-4464 -LRB- denotes (
T9829 4459-4462 NN denotes kit
T9828 4449-4458 NN denotes isolation
T9827 4445-4448 NN denotes RNA
T9826 4442-4444 DT denotes an
T9825 4436-4441 VB denotes using
T9824 4430-4435 NN denotes cells
T9823 4428-4429 NN denotes T
T9822 4423-4427 JJ denotes CD4+
T9821 4420-4422 NN denotes PB
T9820 4415-4419 IN denotes from
T9819 4405-4414 VB denotes extracted
T9818 4401-4404 VB denotes was
T9817 4397-4400 NN denotes RNA
T9816 4388-4396 JJ denotes cellular
T9815 4382-4387 JJ denotes Total
T9814 4378-4381 NN denotes PCR
T9813 4368-4377 JJ denotes real-time
T9812 4364-4367 CC denotes and
T9811 4359-4363 NN denotes mRNA
T9810 4356-4358 IN denotes of
T9809 4346-4355 NN denotes Isolation
T9121 4019-4021 IN denotes in
T9120 4014-4018 NN denotes IL-2
T9119 4010-4013 CC denotes and
T9118 4004-4009 NN denotes IFN-γ
T9117 4001-4003 IN denotes of
T9116 3986-4000 NN denotes Concentrations
T9115 3981-3985 NN denotes IL-2
T9114 3977-3980 CC denotes and
T9113 3971-3976 NN denotes IFN-γ
T9112 3967-3970 IN denotes for
T9111 3955-3966 NN denotes Immunoassay
T10012 5285-5288 IN denotes for
T10011 5283-5284 -RRB- denotes )
T10010 5282-5283 NN denotes s
T10009 5279-5281 CD denotes 10
T10008 5278-5279 -LRB- denotes (
T10007 5276-5277 NN denotes C
T10006 5272-5275 CD denotes 72°
T10005 5269-5271 IN denotes at
T10004 5265-5268 CC denotes and
T10003 5263-5264 -COMMA- denotes ,
T10002 5262-5263 -RRB- denotes )
T10001 5261-5262 NN denotes s
T10000 5258-5260 CD denotes 15
T9999 5257-5258 -LRB- denotes (
T9998 5255-5256 NN denotes C
T9997 5251-5254 CD denotes 62°
T9996 5248-5250 IN denotes at
T9995 5246-5247 -COMMA- denotes ,
T9994 5245-5246 -RRB- denotes )
T9993 5244-5245 NN denotes s
T9992 5241-5243 CD denotes 10
T9991 5240-5241 -LRB- denotes (
T9990 5238-5239 NN denotes C
T9989 5234-5237 CD denotes 95°
T9988 5231-5233 IN denotes at
T9987 5229-5230 -COLON- denotes ;
T9986 5222-5229 NN denotes β-actin
T9985 5218-5221 IN denotes for
T9984 5216-5217 -RRB- denotes )
T9983 5215-5216 NN denotes s
T9982 5212-5214 CD denotes 22
T9981 5211-5212 -LRB- denotes (
T9980 5209-5210 NN denotes C
T9979 5205-5208 NN denotes 72°
T9978 5201-5204 CC denotes and
T9977 5199-5200 -COMMA- denotes ,
T9976 5198-5199 -RRB- denotes )
T9975 5197-5198 NN denotes s
T9974 5194-5196 CD denotes 15
T9973 5193-5194 -LRB- denotes (
T9972 5191-5192 NN denotes C
T9971 5187-5190 CD denotes 65°
T9970 5184-5186 IN denotes at
T9969 5182-5183 -COMMA- denotes ,
T9968 5181-5182 -RRB- denotes )
T9967 5180-5181 NN denotes s
T9966 5177-5179 CD denotes 10
T9965 5176-5177 -LRB- denotes (
T9964 5174-5175 NN denotes C
T9963 5170-5173 CD denotes 95°
T9962 5167-5169 IN denotes at
T8833 3951-3952 -RRB- denotes )
T8832 3942-3951 NNP denotes Dickinson
T8831 3935-3941 NNP denotes Becton
T8830 3934-3935 -LRB- denotes (
T8829 3924-3933 NN denotes cytometer
T8828 3919-3923 NN denotes flow
T8827 3911-3918 NN denotes FACScan
T8826 3909-3910 DT denotes a
T8825 3906-3908 IN denotes on
T8824 3896-3905 VB denotes performed
T8823 3892-3895 VB denotes was
T8822 3883-3891 NN denotes Analysis
T8821 3870-3881 NN denotes incubations
T8820 3862-3869 IN denotes between
T8819 3854-3861 NN denotes FCS/PBS
T8818 3852-3853 NN denotes %
T8817 3851-3852 CD denotes 1
T8816 3846-3850 IN denotes with
T8815 3841-3845 RB denotes well
T8814 3834-3840 VB denotes washed
T8813 3829-3833 VB denotes were
T8812 3823-3828 NN denotes Cells
T8811 3820-3821 -RRB- denotes )
T8810 3811-3820 NNP denotes Dickinson
T8809 3804-3810 NNP denotes Becton
T8808 3803-3804 -LRB- denotes (
T8807 3799-3802 NN denotes mAb
T8806 3790-3798 JJ denotes anti-CD4
T8805 3765-3789 JJ denotes phycoerythrin-conjugated
T8804 3760-3764 IN denotes with
T8803 3750-3759 VB denotes incubated
T8802 3745-3749 RB denotes then
T8801 3740-3744 VB denotes were
T8800 3734-3739 NN denotes Cells
T8799 3731-3732 -RRB- denotes )
T8798 3728-3731 NNP denotes USA
T8797 3726-3727 -COMMA- denotes ,
T8796 3724-3726 NNP denotes CA
T8795 3722-3723 -COMMA- denotes ,
T8794 3718-3722 NNP denotes Cruz
T8793 3712-3717 NNP denotes Santa
T8792 3710-3711 -COMMA- denotes ,
T8791 3695-3710 NNP denotes Biotechnologies
T8790 3690-3694 NNP denotes Cruz
T8789 3684-3689 NNP denotes Santa
T8788 3683-3684 -LRB- denotes (
T8787 3674-3682 NN denotes antibody
T8786 3663-3673 JJ denotes polyclonal
T8785 3658-3662 NN denotes IgG1
T8784 3647-3657 JJ denotes anti-mouse
T8783 3642-3646 NN denotes goat
T8782 3626-3641 JJ denotes FITC-conjugated
T8781 3621-3625 IN denotes with
T8780 3610-3620 NN denotes incubation
T8779 3607-3609 IN denotes by
T8778 3598-3606 VB denotes followed
T8777 3596-3597 -COMMA- denotes ,
T8776 3595-3596 -RRB- denotes )
T8775 3585-3595 NN denotes Immunotech
T8774 3584-3585 -LRB- denotes (
T8773 3580-3583 NN denotes mAb
T8772 3572-3579 NN denotes control
T8771 3556-3571 JJ denotes isotype-matched
T8770 3551-3555 IN denotes with
T8769 3548-3550 CC denotes or
T8768 3546-3547 -RRB- denotes )
T8767 3543-3546 NNP denotes USA
T8766 3541-3542 -COMMA- denotes ,
T8765 3539-3541 NNP denotes MN
T8764 3537-3538 -COMMA- denotes ,
T8763 3526-3537 NNP denotes Minneapolis
T8762 3524-3525 -COMMA- denotes ,
T8761 3517-3524 NN denotes systems
T8760 3515-3516 NN denotes D
T8759 3514-3515 CC denotes &
T8758 3513-3514 NN denotes R
T8757 3511-3512 -COLON- denotes ;
T8756 3507-3511 NN denotes IgG1
T8755 3506-3507 -LRB- denotes (
T8754 3502-3505 NN denotes mAb
T8753 3489-3501 JJ denotes anti-IL-10R1
T8752 3486-3488 IN denotes of
T8751 3471-3485 NN denotes concentrations
T8750 3460-3470 VB denotes saturating
T8749 3455-3459 IN denotes with
T8748 3445-3454 VB denotes incubated
T8747 3440-3444 VB denotes were
T8746 3435-3439 NN denotes PBMC
T8745 3430-3433 NN denotes FCS
T8744 3428-3429 NN denotes %
T8743 3427-3428 CD denotes 1
T8742 3422-3426 IN denotes with
T8741 3418-3421 NNP denotes PBS
T8740 3415-3417 IN denotes in
T8739 3403-3414 VB denotes resuspended
T8738 3399-3402 VB denotes was
T8737 3394-3398 NN denotes PBMC
T8736 3391-3393 IN denotes of
T8735 3385-3390 NN denotes cells
T8734 3381-3384 CD denotes 105
T8733 3379-3380 IN denotes ×
T8732 3377-3378 CD denotes 5
T8731 3374-3376 IN denotes of
T8730 3367-3373 NN denotes sample
T8729 3365-3366 DT denotes A
T8728 3354-3364 NN denotes expression
T8727 3346-3353 NN denotes IL-10R1
T8726 3342-3345 IN denotes for
T8725 3333-3341 NN denotes analysis
T8724 3322-3332 JJ denotes cytometric
T8723 3317-3321 NN denotes Flow
T9170 4338-4343 NN denotes pg/ml
T9169 4335-4337 CD denotes 15
T9168 4330-4334 VB denotes were
T9167 4325-4329 NN denotes IL-2
T9166 4321-4324 CC denotes and
T9165 4315-4320 NN denotes IFN-γ
T9164 4311-4314 IN denotes for
T9163 4304-4310 NN denotes limits
T9162 4294-4303 NN denotes detection
T9161 4290-4293 DT denotes The
T9160 4280-4288 NN denotes protocol
T9159 4277-4279 POS denotes 's
T9158 4265-4277 NN denotes manufacturer
T9157 4261-4264 DT denotes the
T9156 4258-4260 TO denotes to
T9155 4248-4257 VB denotes according
T9154 4246-4247 -COMMA- denotes ,
T9153 4245-4246 -RRB- denotes )
T9152 4236-4245 NNP denotes Dickinson
T9151 4229-4235 NNP denotes Becton
T9150 4224-4228 IN denotes from
T9149 4220-4223 DT denotes all
T9148 4219-4220 -LRB- denotes (
T9147 4210-4218 NN denotes proteins
T9146 4201-4209 NN denotes cytokine
T9145 4189-4200 JJ denotes recombinant
T9144 4185-4188 CC denotes and
T9143 4181-4184 NN denotes mAb
T9142 4171-4180 NN denotes detection
T9141 4158-4170 VB denotes biotinylated
T9140 4153-4157 IN denotes with
T9139 4145-4152 NN denotes capture
T9138 4127-4144 JJ denotes cytokine-specific
T9137 4121-4126 VB denotes using
T9136 4115-4120 NN denotes ELISA
T9135 4106-4114 NN denotes sandwich
T9134 4093-4105 JJ denotes quantitative
T9133 4089-4092 DT denotes the
T9132 4086-4088 IN denotes by
T9131 4076-4085 NN denotes duplicate
T9130 4073-4075 IN denotes in
T9129 4064-4072 VB denotes measured
T9128 4059-4063 VB denotes were
T9127 4053-4058 NN denotes cells
T9126 4051-4052 NN denotes T
T9125 4046-4050 JJ denotes CD4+
T9124 4043-4045 IN denotes of
T9123 4030-4042 NN denotes supernatants
T9122 4022-4029 NN denotes culture
T8296 3276-3280 VB denotes were
T8295 3263-3275 NN denotes supernatants
T8294 3255-3262 NN denotes Culture
T8293 3248-3253 NN denotes IL-10
T8292 3242-3247 NN denotes ng/ml
T8291 3240-3241 CD denotes 1
T8290 3237-3239 IN denotes of
T8289 3229-3236 NN denotes absence
T8288 3226-3228 CC denotes or
T8287 3217-3225 NN denotes presence
T8286 3213-3216 DT denotes the
T8285 3210-3212 IN denotes in
T8284 3206-3209 NN denotes mAb
T8283 3196-3205 JJ denotes anti-CD28
T8282 3192-3195 CC denotes and
T8281 3188-3191 NN denotes mAb
T8280 3179-3187 JJ denotes anti-CD3
T8279 3174-3178 IN denotes with
T8278 3168-3173 NN denotes hours
T8277 3165-3167 CD denotes 36
T8276 3161-3164 IN denotes for
T8275 3150-3160 VB denotes stimulated
T8274 3145-3149 RB denotes then
T8273 3140-3144 VB denotes were
T8272 3134-3139 NN denotes Cells
T8271 3127-3132 NN denotes hours
T8270 3124-3126 CD denotes 36
T8269 3120-3123 IN denotes for
T8268 3118-3119 -RRB- denotes )
T8267 3109-3118 NNP denotes Dickinson
T8266 3102-3108 NNP denotes Becton
T8265 3101-3102 -LRB- denotes (
T8264 3096-3100 NN denotes IL-6
T8263 3090-3095 NN denotes ng/ml
T8262 3087-3089 CD denotes 10
T8261 3084-3086 IN denotes of
T8260 3076-3083 NN denotes absence
T8259 3073-3075 CC denotes or
T8258 3064-3072 NN denotes presence
T8257 3060-3063 DT denotes the
T8256 3057-3059 IN denotes in
T8255 3053-3056 NN denotes FCS
T8254 3051-3052 NN denotes %
T8253 3049-3051 CD denotes 10
T8252 3044-3048 IN denotes with
T8251 3037-3043 NN denotes medium
T8250 3029-3036 NN denotes culture
T8249 3026-3028 IN denotes in
T8248 3016-3025 VB denotes incubated
T8247 3011-3015 VB denotes were
T8246 3002-3010 NN denotes controls
T8245 2994-3001 JJ denotes healthy
T8244 2989-2993 IN denotes from
T8243 2983-2988 NN denotes cells
T8242 2981-2982 NN denotes T
T8241 2976-2980 JJ denotes CD4+
T8240 2974-2975 -COMMA- denotes ,
T8239 2969-2974 NN denotes IL-10
T8238 2966-2968 TO denotes to
T8237 2951-2965 NN denotes responsiveness
T8236 2944-2950 NN denotes T-cell
T8235 2941-2943 IN denotes on
T8234 2936-2940 NN denotes IL-6
T8233 2933-2935 IN denotes of
T8232 2926-2932 NN denotes effect
T8231 2922-2925 DT denotes the
T8230 2914-2921 VB denotes examine
T8229 2911-2913 TO denotes To
T8228 2904-2909 NN denotes assay
T8227 2895-2903 NN denotes cytokine
T8226 2889-2894 IN denotes until
T8225 2883-2888 NN denotes -30°C
T8224 2880-2882 IN denotes at
T8223 2873-2879 VB denotes stored
T8222 2868-2872 VB denotes were
T8221 2860-2867 NN denotes samples
T8220 2850-2859 JJ denotes cell-free
T8219 2846-2849 CC denotes and
T8218 2840-2845 RB denotes later
T8217 2834-2839 NN denotes hours
T8216 2831-2833 CD denotes 36
T8215 2821-2830 VB denotes collected
T8214 2816-2820 VB denotes were
T8213 2803-2815 NN denotes supernatants
T8212 2795-2802 NN denotes Culture
T8211 2792-2793 -RRB- denotes ]
T8210 2790-2792 CD denotes 28
T8209 2789-2790 -LRB- denotes [
T8208 2785-2788 NN denotes CO2
T8207 2783-2784 NN denotes %
T8206 2782-2783 CD denotes 5
T8205 2771-2781 VB denotes containing
T8204 2760-2770 NN denotes atmosphere
T8203 2749-2759 JJ denotes humidified
T8202 2747-2748 DT denotes a
T8201 2744-2746 IN denotes in
T8200 2739-2743 NN denotes 37°C
T8199 2736-2738 IN denotes at
T8198 2734-2735 -RRB- denotes )
T8197 2731-2734 NNP denotes USA
T8196 2729-2730 -COMMA- denotes ,
T8195 2727-2729 NNP denotes CA
T8194 2725-2726 -COMMA- denotes ,
T8193 2721-2725 NNP denotes Jose
T8192 2717-2720 NNP denotes San
T8191 2715-2716 -COMMA- denotes ,
T8190 2706-2715 NNP denotes Dickinson
T8189 2699-2705 NNP denotes Becton
T8188 2698-2699 -LRB- denotes (
T8187 2692-2697 NN denotes IL-10
T8186 2689-2691 IN denotes of
T8185 2674-2688 NN denotes concentrations
T8184 2664-2673 VB denotes indicated
T8183 2660-2663 DT denotes the
T8182 2657-2659 IN denotes of
T8181 2649-2656 NN denotes absence
T8180 2646-2648 CC denotes or
T8179 2637-2645 NN denotes presence
T8178 2633-2636 DT denotes the
T8177 2630-2632 IN denotes in
T8176 2628-2629 -RRB- denotes )
T8175 2618-2628 NN denotes Immunotech
T8174 2617-2618 -LRB- denotes (
T8173 2613-2616 NN denotes mAb
T8172 2603-2612 JJ denotes anti-CD28
T8171 2597-2602 NN denotes μg/ml
T8170 2595-2596 CD denotes 1
T8169 2590-2594 IN denotes with
T8168 2580-2589 VB denotes incubated
T8167 2575-2579 VB denotes were
T8166 2569-2574 NN denotes cells
T8165 2565-2568 DT denotes The
T8164 2562-2563 -RRB- denotes )
T8163 2556-2562 NNP denotes France
T8162 2554-2555 -COMMA- denotes ,
T8161 2545-2554 NNP denotes Marseille
T8160 2543-2544 -COMMA- denotes ,
T8159 2533-2543 NNP denotes Immunotech
T8158 2532-2533 -LRB- denotes (
T8157 2528-2531 NN denotes mAb
T8156 2519-2527 JJ denotes anti-CD3
T8155 2513-2518 NN denotes μg/ml
T8154 2511-2512 CD denotes 1
T8153 2506-2510 IN denotes with
T8152 2499-2505 VB denotes coated
T8151 2497-2498 -RRB- denotes )
T8150 2491-2497 NN denotes Coster
T8149 2490-2491 -LRB- denotes (
T8148 2483-2489 NN denotes plates
T8147 2472-2482 NN denotes microtiter
T8146 2464-2471 JJ denotes 24-well
T8145 2461-2463 IN denotes of
T8144 2455-2460 NN denotes wells
T8143 2451-2454 DT denotes the
T8142 2446-2450 IN denotes into
T8141 2436-2445 VB denotes dispensed
T8140 2431-2435 VB denotes were
T8139 2419-2430 NN denotes suspensions
T8138 2414-2418 NN denotes cell
T8137 2411-2413 NN denotes ml
T8136 2407-2410 CD denotes 0.5
T8135 2403-2406 CC denotes and
T8134 2401-2402 -COMMA- denotes ,
T8133 2398-2401 NN denotes FCS
T8132 2396-2397 NN denotes %
T8131 2394-2396 CD denotes 10
T8130 2389-2393 IN denotes with
T8129 2382-2388 NN denotes medium
T8128 2374-2381 NN denotes culture
T8127 2371-2373 IN denotes in
T8126 2362-2370 NN denotes cells/ml
T8125 2358-2361 CD denotes 106
T8124 2356-2357 NN denotes ×
T8123 2354-2355 CD denotes 1
T8122 2351-2353 IN denotes of
T8121 2343-2350 NN denotes density
T8120 2341-2342 DT denotes a
T8119 2338-2340 IN denotes at
T8118 2326-2337 VB denotes resuspended
T8117 2321-2325 VB denotes were
T8116 2309-2320 NN denotes populations
T8115 2302-2308 NN denotes T-cell
T8114 2297-2301 JJ denotes CD4+
T8113 2294-2296 NN denotes PB
T8112 2288-2293 NN denotes cells
T8111 2286-2287 NN denotes T
T8110 2281-2285 JJ denotes CD4+
T8109 2278-2280 IN denotes of
T8108 2270-2277 NN denotes Culture
T8300 3300-3314 NN denotes concentrations
T8299 3294-3299 NN denotes IFN-γ
T8298 3290-3293 IN denotes for
T8297 3281-3289 VB denotes measured
T7701 2258-2267 VB denotes described
T7700 2250-2257 RB denotes already
T7699 2247-2249 IN denotes as
T7698 2237-2246 NN denotes selection
T7697 2228-2236 JJ denotes positive
T7696 2225-2227 IN denotes by
T7695 2216-2224 VB denotes purified
T7694 2211-2215 VB denotes were
T7693 2205-2210 NN denotes cells
T7692 2203-2204 NN denotes T
T7691 2198-2202 JJ denotes CD4+
T7690 2194-2197 CC denotes and
T7689 2184-2193 VB denotes harvested
T7688 2179-2183 VB denotes were
T7687 2173-2178 NN denotes cells
T7686 2160-2172 JJ denotes Non-adherent
T7685 2155-2158 NN denotes min
T7684 2152-2154 CD denotes 45
T7683 2148-2151 IN denotes for
T7682 2146-2147 -RRB- denotes )
T7681 2143-2146 NNP denotes USA
T7680 2141-2142 -COMMA- denotes ,
T7679 2139-2141 NNP denotes MA
T7678 2137-2138 -COMMA- denotes ,
T7677 2128-2137 NNP denotes Cambridge
T7676 2126-2127 -COMMA- denotes ,
T7675 2120-2126 NNP denotes Coster
T7674 2119-2120 -LRB- denotes (
T7673 2112-2118 NN denotes plates
T7672 2103-2111 JJ denotes six-well
T7671 2100-2102 IN denotes in
T7670 2095-2099 NN denotes 37°C
T7669 2092-2094 IN denotes at
T7668 2082-2091 VB denotes incubated
T7667 2077-2081 VB denotes were
T7666 2075-2076 -RRB- denotes )
T7665 2063-2075 NNP denotes Technologies
T7664 2058-2062 NNP denotes Life
T7663 2057-2058 -LRB- denotes (
T7662 2053-2056 NN denotes FCS
T7661 2036-2052 JJ denotes heat-inactivated
T7660 2034-2035 NN denotes %
T7659 2032-2034 CD denotes 10
T7658 2027-2031 IN denotes with
T7657 2025-2026 -RRB- denotes )
T7656 2013-2025 NNP denotes Technologies
T7655 2008-2012 NNP denotes Life
T7654 2006-2007 -COLON- denotes ;
T7653 1994-2006 NN denotes streptomycin
T7652 1988-1993 NN denotes mg/ml
T7651 1984-1987 CD denotes 100
T7650 1980-1983 CC denotes and
T7649 1978-1979 -COMMA- denotes ,
T7648 1968-1978 NN denotes penicillin
T7647 1962-1967 NN denotes IU/ml
T7646 1958-1961 CD denotes 100
T7645 1956-1957 -COMMA- denotes ,
T7644 1951-1956 NN denotes acids
T7643 1945-1950 NN denotes amino
T7642 1932-1944 JJ denotes nonessential
T7641 1930-1931 NN denotes %
T7640 1929-1930 CD denotes 2
T7639 1927-1928 -COMMA- denotes ,
T7638 1916-1927 NN denotes L-glutamine
T7637 1913-1915 NN denotes mM
T7636 1911-1912 CD denotes 2
T7635 1910-1911 -LRB- denotes (
T7634 1904-1909 NN denotes HEPES
T7633 1901-1903 NN denotes mM
T7632 1898-1900 CD denotes 25
T7631 1893-1897 IN denotes with
T7630 1880-1892 VB denotes supplemented
T7629 1878-1879 -RRB- denotes )
T7628 1875-1878 NNP denotes USA
T7627 1873-1874 -COMMA- denotes ,
T7626 1871-1873 NNP denotes MD
T7625 1869-1870 -COMMA- denotes ,
T7554 1465-1475 JJ denotes mAb-coated
T7553 1456-1464 JJ denotes anti-CD4
T7552 1450-1455 VB denotes using
T7551 1440-1449 NN denotes selection
T7550 1431-1439 JJ denotes positive
T7549 1428-1430 IN denotes by
T7548 1423-1427 NN denotes PBMC
T7547 1418-1422 IN denotes from
T7546 1409-1417 VB denotes purified
T7545 1404-1408 VB denotes were
T7544 1398-1403 NN denotes cells
T7543 1396-1397 NN denotes T
T7542 1391-1395 JJ denotes CD4+
T7541 1388-1389 -RRB- denotes )
T7540 1382-1388 NNP denotes Sweden
T7539 1380-1381 -COMMA- denotes ,
T7538 1373-1380 NNP denotes Uppsala
T7537 1371-1372 -COMMA- denotes ,
T7536 1362-1371 NNP denotes Pharmacia
T7535 1361-1362 -LRB- denotes (
T7534 1351-1360 NN denotes gradients
T7533 1343-1350 NN denotes density
T7532 1328-1342 JJ denotes Ficoll-Hypaque
T7531 1323-1327 IN denotes over
T7530 1308-1322 NN denotes centrifugation
T7529 1305-1307 IN denotes by
T7528 1297-1304 NN denotes samples
T7527 1291-1296 NN denotes blood
T7526 1279-1290 VB denotes heparinized
T7525 1274-1278 IN denotes from
T7524 1265-1273 VB denotes prepared
T7523 1260-1264 VB denotes were
T7522 1258-1259 -RRB- denotes )
T7521 1254-1258 NN denotes PBMC
T7520 1253-1254 -LRB- denotes (
T7519 1247-1252 NN denotes cells
T7518 1235-1246 JJ denotes mononuclear
T7517 1229-1234 NN denotes blood
T7516 1218-1228 JJ denotes Peripheral
T7515 1212-1217 NN denotes cells
T7514 1210-1211 NN denotes T
T7513 1205-1209 JJ denotes CD4+
T7512 1202-1204 IN denotes of
T7511 1192-1201 NN denotes Isolation
T7080 1182-1189 NN denotes consent
T7079 1173-1181 JJ denotes informed
T7078 1168-1172 VB denotes gave
T7077 1159-1167 NN denotes patients
T7076 1155-1158 DT denotes All
T7075 1142-1153 NN denotes replacement
T7074 1137-1141 NN denotes knee
T7073 1131-1136 JJ denotes total
T7072 1120-1130 VB denotes undergoing
T7071 1111-1119 NN denotes patients
T7070 1108-1110 NN denotes RA
T7069 1102-1107 CD denotes three
T7068 1097-1101 IN denotes from
T7067 1088-1096 VB denotes obtained
T7066 1083-1087 VB denotes were
T7065 1075-1082 NN denotes samples
T7064 1072-1074 NN denotes ST
T7063 1062-1070 NN denotes controls
T7062 1059-1061 IN denotes as
T7061 1052-1058 VB denotes served
T7060 1050-1051 -RRB- denotes )
T7059 1045-1050 NN denotes years
T7058 1040-1044 CD denotes 11.2
T7057 1038-1039 CC denotes ±
T7056 1033-1037 CD denotes 45.8
T7055 1031-1032 -COMMA- denotes ,
T7054 1028-1031 NN denotes age
T7053 1026-1027 -COLON- denotes ;
T7052 1023-1026 NN denotes men
T7051 1018-1022 CD denotes five
T7050 1014-1017 CC denotes and
T7049 1008-1013 NN denotes women
T7624 1857-1869 NNP denotes Gaithersburg
T7623 1855-1856 -COMMA- denotes ,
T7622 1843-1855 NNP denotes Technologies
T7621 1838-1842 NNP denotes Life
T7620 1836-1837 -COLON- denotes ;
T7619 1830-1836 NN denotes medium
T7618 1825-1829 CD denotes 1640
T7617 1820-1824 NN denotes RPMI
T7616 1819-1820 -LRB- denotes (
T7615 1812-1818 NN denotes medium
T7614 1804-1811 NN denotes culture
T7613 1801-1803 IN denotes in
T7612 1789-1800 NN denotes suspensions
T7611 1784-1788 NN denotes cell
T7610 1781-1783 JJ denotes ST
T7609 1779-1780 -COMMA- denotes ,
T7608 1773-1779 NN denotes debris
T7607 1766-1772 NN denotes tissue
T7606 1757-1765 VB denotes removing
T7605 1751-1756 IN denotes After
T7604 1745-1750 NNP denotes 37°C.
T7603 1742-1744 IN denotes at
T7602 1737-1741 NN denotes hour
T7601 1735-1736 CD denotes 1
T7600 1731-1734 IN denotes for
T7599 1725-1730 NN denotes DNase
T7598 1721-1724 CC denotes and
T7597 1709-1720 NN denotes collagenase
T7596 1704-1708 IN denotes with
T7595 1695-1703 VB denotes digested
T7594 1691-1694 CC denotes and
T7593 1680-1690 VB denotes fragmented
T7592 1675-1679 VB denotes were
T7591 1667-1674 NN denotes samples
T7590 1664-1666 NN denotes ST
T7589 1658-1663 JJ denotes fresh
T7588 1656-1657 -COMMA- denotes ,
T7587 1649-1656 RB denotes Briefly
T7586 1646-1647 -RRB- denotes ]
T7585 1644-1646 CD denotes 27
T7584 1643-1644 -LRB- denotes [
T7583 1633-1642 VB denotes described
T7582 1622-1632 RB denotes previously
T7581 1619-1621 IN denotes as
T7580 1617-1618 -COMMA- denotes ,
T7579 1610-1617 NN denotes samples
T7578 1607-1609 NN denotes ST
T7577 1602-1606 IN denotes from
T7576 1593-1601 VB denotes isolated
T7575 1588-1592 VB denotes were
T7574 1582-1587 NN denotes cells
T7573 1580-1581 NN denotes T
T7572 1575-1579 JJ denotes CD4+
T7571 1561-1573 NN denotes instructions
T7570 1558-1560 POS denotes 's
T7569 1546-1558 NN denotes manufacturer
T7568 1542-1545 DT denotes the
T7567 1539-1541 TO denotes to
T7566 1529-1538 VB denotes according
T7565 1527-1528 -COMMA- denotes ,
T7564 1526-1527 -RRB- denotes )
T7563 1519-1526 NNP denotes Germany
T7562 1517-1518 -COMMA- denotes ,
T7561 1509-1517 NNP denotes Gladbach
T7560 1507-1508 -COMMA- denotes ,
T7559 1501-1507 NNP denotes Biotec
T7558 1492-1500 NNP denotes Miltenyi
T7557 1491-1492 -LRB- denotes (
T7556 1485-1490 NN denotes beads
T7555 1476-1484 JJ denotes magnetic
T7048 1005-1007 CD denotes 11
T7047 1004-1005 -LRB- denotes (
T7046 993-1003 NN denotes volunteers
T7045 985-992 JJ denotes healthy
T7044 977-984 CD denotes Sixteen
T7043 974-975 -RRB- denotes ]
T7042 972-974 CD denotes 26
T7041 971-972 -LRB- denotes [
T7040 961-970 NN denotes remission
T7039 952-960 JJ denotes clinical
T7038 948-951 IN denotes for
T7037 939-947 NN denotes criteria
T7036 927-938 JJ denotes preliminary
T7035 914-926 NNP denotes Rheumatology
T7034 911-913 IN denotes of
T7033 903-910 NNP denotes College
T7032 894-902 NNP denotes American
T7031 890-893 DT denotes the
T7030 880-889 VB denotes satisfied
T7029 876-879 WP denotes who
T7028 874-875 -COMMA- denotes ,
T7027 867-874 NN denotes disease
T7026 858-866 JJ denotes inactive
T7025 853-857 IN denotes with
T7024 844-852 NN denotes patients
T7023 838-843 CD denotes eight
T7022 834-837 CC denotes and
T7021 832-833 -COLON- denotes ;
T7020 831-832 -RRB- denotes )
T7019 827-831 NN denotes mg/l
T7018 824-826 CD denotes 10
T7017 822-823 NN denotes ≥
T6952 482-486 JJ denotes mean
T6951 481-482 -LRB- denotes (
T6950 473-480 VB denotes follows
T6949 470-472 RB denotes as
T6948 465-469 VB denotes were
T6947 456-464 NN denotes patients
T6946 450-455 NN denotes study
T6945 446-449 DT denotes the
T6944 443-445 IN denotes in
T6943 432-442 NN denotes parameters
T6942 423-431 JJ denotes Clinical
T6941 416-421 NN denotes drugs
T6940 402-415 JJ denotes antirheumatic
T6939 384-401 JJ denotes disease-modifying
T6938 380-383 CC denotes and
T6937 378-379 -RRB- denotes )
T6936 372-378 NN denotes mg/day
T6935 368-371 CD denotes 7.5
T6934 366-367 SYM denotes ≤
T6933 365-366 -LRB- denotes (
T6932 352-364 NN denotes prednisolone
T6931 342-351 VB denotes receiving
T6930 337-341 VB denotes were
T6929 328-336 NN denotes patients
T6928 324-327 DT denotes All
T6927 321-322 -RRB- denotes ]
T6926 319-321 CD denotes 25
T6925 318-319 -LRB- denotes [
T6924 316-317 -RRB- denotes )
T6923 305-316 NNP denotes Association
T6922 294-304 NNP denotes Rheumatism
T6921 285-293 NNP denotes American
T6920 281-284 DT denotes the
T6919 279-280 -COMMA- denotes ,
T6918 271-279 RB denotes formally
T6917 270-271 -LRB- denotes (
T6916 257-269 NNP denotes Rheumatology
T6915 254-256 IN denotes of
T6914 246-253 NNP denotes College
T6913 237-245 NNP denotes American
T6912 233-236 DT denotes the
T6911 230-232 IN denotes of
T6910 221-229 NN denotes criteria
T6909 216-220 CD denotes 1987
T6908 208-215 VB denotes revised
T6907 204-207 DT denotes the
T6906 201-203 TO denotes to
T6905 191-200 VB denotes according
T6904 181-190 VB denotes diagnosed
T6903 179-180 -RRB- denotes )
T6902 174-179 NN denotes years
T6901 169-173 CD denotes 12.4
T6900 167-168 CC denotes ±
T6899 162-166 CD denotes 52.8
T6898 160-161 -COMMA- denotes ,
T6897 157-160 NN denotes age
T6896 147-156 NN denotes deviation
T6895 138-146 JJ denotes standard
T6894 136-137 JJ denotes ±
T6893 131-135 JJ denotes mean
T6892 129-130 -COLON- denotes ;
T6891 126-129 NN denotes men
T6890 120-125 CD denotes seven
T6889 116-119 CC denotes and
T6888 110-115 NN denotes women
T6887 107-109 CD denotes 25
T6886 106-107 -LRB- denotes (
T6885 103-105 NN denotes RA
T6884 98-102 IN denotes with
T6883 89-97 NN denotes patients
T6882 86-88 CD denotes 32
T6881 83-85 IN denotes of
T6880 73-82 VB denotes consisted
T6879 62-72 NN denotes population
T6878 54-61 NN denotes patient
T6877 48-53 JJ denotes total
T6876 44-47 DT denotes The
T6875 36-43 NN denotes samples
T6874 32-35 CC denotes and
T6873 23-31 NN denotes Patients
T7016 821-822 -LRB- denotes (
T7015 815-820 NN denotes level
T7014 811-814 NN denotes CRP
T7013 805-810 NN denotes serum
T7012 796-804 VB denotes elevated
T7011 792-795 CC denotes and
T7010 785-791 NN denotes joints
T7009 777-784 VB denotes swollen
T7008 770-776 CC denotes and/or
T7007 763-769 NN denotes tender
T7006 754-762 JJ denotes multiple
T7005 750-753 VB denotes had
T7004 746-749 WP denotes who
T7003 744-745 -COMMA- denotes ,
T7002 737-744 NN denotes disease
T7001 730-736 JJ denotes active
T7000 725-729 IN denotes with
T6999 716-724 NN denotes patients
T6998 713-715 CD denotes 24
T6997 711-712 -COLON- denotes :
T6996 705-711 NN denotes groups
T6995 701-704 CD denotes two
T6994 696-700 IN denotes into
T6993 688-695 VB denotes divided
T6992 683-687 VB denotes were
T6991 674-682 NN denotes Patients
T6990 668-672 NN denotes U/ml
T6989 664-667 CD denotes 158
T6988 662-663 -COLON- denotes ±
T6987 658-661 CD denotes 142
T6986 656-657 -COMMA- denotes ,
T6985 651-656 NN denotes titer
T6984 644-650 NN denotes factor
T6983 633-643 JJ denotes rheumatoid
T6982 627-632 NN denotes class
T6981 623-626 NN denotes IgM
T6980 619-622 CC denotes and
T6979 617-618 -COLON- denotes ;
T6978 613-617 NN denotes mg/l
T6977 608-612 CD denotes 32.0
T6976 606-607 -COLON- denotes ±
T6975 601-605 CD denotes 32.0
T6974 599-600 -COMMA- denotes ,
T6973 594-599 NN denotes level
T6972 592-593 -RRB- denotes )
T6971 589-592 NN denotes CRP
T6970 588-589 -LRB- denotes (
T6969 580-587 NN denotes protein
T6968 569-579 JJ denotes C-reactive
T6967 563-568 NN denotes serum
T6966 561-562 -COLON- denotes ;
T6965 554-561 NN denotes mm/hour
T6964 549-553 CD denotes 35.4
T6963 547-548 -COLON- denotes ±
T6962 542-546 CD denotes 55.9
T6961 540-541 -COMMA- denotes ,
T6960 536-540 NN denotes rate
T6959 522-535 NN denotes sedimentation
T6958 510-521 NN denotes erythrocyte
T6957 508-509 -COLON- denotes :
T6956 507-508 -RRB- denotes )
T6955 498-507 NN denotes deviation
T6954 489-497 JJ denotes standard
T6953 487-488 JJ denotes ±
R3193 T6873 T6874 arg1Of Patients,and
R3194 T6875 T6874 arg2Of samples,and
R3195 T6879 T6876 arg1Of population,The
R3196 T6879 T6877 arg1Of population,total
R3197 T6879 T6878 arg1Of population,patient
R3198 T6879 T6880 arg1Of population,consisted
R3199 T6880 T6881 arg1Of consisted,of
R3200 T6880 T6925 arg1Of consisted,[
R3201 T6883 T6881 arg2Of patients,of
R3202 T6883 T6882 arg1Of patients,32
R3203 T6883 T6884 arg1Of patients,with
R3204 T6883 T6886 arg1Of patients,(
R3205 T6883 T6904 arg2Of patients,diagnosed
R3206 T6883 T6915 arg1Of patients,of
R3207 T6885 T6884 arg2Of RA,with
R3208 T6888 T6887 arg1Of women,25
R3209 T6888 T6889 arg1Of women,and
R3210 T6889 T6886 arg2Of and,(
R3211 T6889 T6892 arg1Of and,;
R3212 T6891 T6889 arg2Of men,and
R3213 T6891 T6890 arg1Of men,seven
R3214 T6897 T6892 arg2Of age,;
R3215 T6897 T6893 arg1Of age,mean
R3216 T6897 T6894 arg1Of age,±
R3217 T6897 T6895 arg1Of age,standard
R3218 T6897 T6896 arg1Of age,deviation
R3219 T6897 T6898 arg1Of age,","
R3220 T6899 T6900 arg1Of 52.8,±
R3221 T6901 T6900 arg2Of 12.4,±
R3222 T6902 T6898 arg2Of years,","
R3223 T6902 T6899 arg1Of years,52.8
R3224 T6902 T6901 arg1Of years,12.4
R3225 T6903 T6886 arg3Of ),(
R3226 T6904 T6905 arg1Of diagnosed,according
R3227 T6906 T6905 arg2Of to,according
R3228 T6910 T6906 arg2Of criteria,to
R3229 T6910 T6907 arg1Of criteria,the
R3230 T6910 T6908 arg2Of criteria,revised
R3231 T6910 T6909 arg1Of criteria,1987
R3232 T6910 T6911 arg1Of criteria,of
R3233 T6914 T6911 arg2Of College,of
R3234 T6914 T6912 arg1Of College,the
R3235 T6914 T6913 arg1Of College,American
R3236 T6916 T6915 arg2Of Rheumatology,of
R3237 T6916 T6917 arg1Of Rheumatology,(
R3238 T6923 T6917 arg2Of Association,(
R3239 T6923 T6918 arg1Of Association,formally
R3240 T6923 T6919 arg1Of Association,","
R3241 T6923 T6920 arg1Of Association,the
R3242 T6923 T6921 arg1Of Association,American
R3243 T6923 T6922 arg1Of Association,Rheumatism
R3244 T6924 T6917 arg3Of ),(
R3245 T6926 T6925 arg2Of 25,[
R3246 T6927 T6925 arg3Of ],[
R3247 T6929 T6928 arg1Of patients,All
R3248 T6929 T6930 arg1Of patients,were
R3249 T6929 T6931 arg1Of patients,receiving
R3250 T6931 T6930 arg2Of receiving,were
R3251 T6932 T6933 arg1Of prednisolone,(
R3252 T6932 T6938 arg1Of prednisolone,and
R3253 T6935 T6934 arg1Of 7.5,≤
R3254 T6936 T6933 arg2Of mg/day,(
R3255 T6936 T6935 arg1Of mg/day,7.5
R3256 T6937 T6933 arg3Of ),(
R3257 T6938 T6931 arg2Of and,receiving
R3258 T6941 T6938 arg2Of drugs,and
R3259 T6941 T6939 arg1Of drugs,disease-modifying
R3260 T6941 T6940 arg1Of drugs,antirheumatic
R3261 T6943 T6942 arg1Of parameters,Clinical
R3262 T6943 T6944 arg1Of parameters,in
R3263 T6943 T6950 arg1Of parameters,follows
R3264 T6946 T6944 arg2Of study,in
R3265 T6946 T6945 arg1Of study,the
R3266 T6946 T6948 arg2Of study,were
R3267 T6947 T6948 arg1Of patients,were
R3268 T6950 T6949 arg1Of follows,as
R3269 T6950 T6951 arg1Of follows,(
R3270 T6950 T6957 arg1Of follows,:
R3271 T6950 T6965 arg1Of follows,mm/hour
R3272 T6950 T6990 arg1Of follows,U/ml
R3273 T6955 T6951 arg2Of deviation,(
R3274 T6955 T6952 arg1Of deviation,mean
R3275 T6955 T6953 arg1Of deviation,±
R3276 T6955 T6954 arg1Of deviation,standard
R3277 T6956 T6951 arg3Of ),(
R3278 T6960 T6950 arg2Of rate,follows
R3279 T6960 T6958 arg1Of rate,erythrocyte
R3280 T6960 T6959 arg1Of rate,sedimentation
R3281 T6960 T6961 arg1Of rate,","
R3282 T6962 T6961 arg2Of 55.9,","
R3283 T6965 T6963 arg1Of mm/hour,±
R3284 T6965 T6964 arg1Of mm/hour,35.4
R3285 T6965 T6966 arg1Of mm/hour,;
R3286 T6971 T6970 arg2Of CRP,(
R3287 T6972 T6970 arg3Of ),(
R3288 T6973 T6966 arg2Of level,;
R3289 T6973 T6967 arg1Of level,serum
R3290 T6973 T6968 arg1Of level,C-reactive
R3291 T6973 T6969 arg1Of level,protein
R3292 T6973 T6970 arg1Of level,(
R3293 T6973 T6974 arg1Of level,","
R3294 T6973 T6986 arg1Of level,","
R3295 T6975 T6978 arg1Of 32.0,mg/l
R3296 T6975 T6982 arg1Of 32.0,class
R3297 T6978 T6977 arg1Of mg/l,32.0
R3298 T6978 T6980 arg1Of mg/l,and
R3299 T6980 T6976 arg1Of and,±
R3300 T6980 T6979 arg1Of and,;
R3301 T6982 T6980 arg2Of class,and
R3302 T6982 T6981 arg1Of class,IgM
R3303 T6985 T6974 arg2Of titer,","
R3304 T6985 T6975 arg1Of titer,32.0
R3305 T6985 T6983 arg1Of titer,rheumatoid
R3306 T6985 T6984 arg1Of titer,factor
R3307 T6987 T6986 arg2Of 142,","
R3308 T6990 T6988 arg1Of U/ml,±
R3309 T6990 T6989 arg1Of U/ml,158
R3310 T6991 T6992 arg1Of Patients,were
R3311 T6991 T6993 arg2Of Patients,divided
R3312 T6993 T6992 arg2Of divided,were
R3313 T6993 T6994 arg1Of divided,into
R3314 T6993 T6997 arg1Of divided,:
R3315 T6996 T6994 arg2Of groups,into
R3316 T6996 T6995 arg1Of groups,two
R3317 T6999 T6998 arg1Of patients,24
R3318 T6999 T7000 arg1Of patients,with
R3319 T6999 T7003 arg1Of patients,","
R3320 T6999 T7004 arg1Of patients,who
R3321 T6999 T7005 arg1Of patients,had
R3322 T7002 T7000 arg2Of disease,with
R3323 T7002 T7001 arg1Of disease,active
R3324 T7007 T7006 arg1Of tender,multiple
R3325 T7007 T7008 arg1Of tender,and/or
R3326 T7008 T7011 arg1Of and/or,and
R3327 T7010 T7008 arg2Of joints,and/or
R3328 T7010 T7009 arg2Of joints,swollen
R3329 T7011 T7022 arg1Of and,and
R3330 T7015 T7011 arg2Of level,and
R3331 T7015 T7012 arg2Of level,elevated
R3332 T7015 T7013 arg1Of level,serum
R3333 T7015 T7014 arg1Of level,CRP
R3334 T7015 T7016 arg1Of level,(
R3335 T7019 T7016 arg2Of mg/l,(
R3336 T7019 T7017 arg1Of mg/l,≥
R3337 T7019 T7018 arg1Of mg/l,10
R3338 T7020 T7016 arg3Of ),(
R3339 T7022 T7005 arg2Of and,had
R3340 T7022 T7021 arg1Of and,;
R3341 T7022 T7029 arg1Of and,who
R3342 T7022 T7030 arg1Of and,satisfied
R3343 T7024 T7022 arg2Of patients,and
R3344 T7024 T7023 arg1Of patients,eight
R3345 T7024 T7025 arg1Of patients,with
R3346 T7024 T7028 arg1Of patients,","
R3347 T7027 T7025 arg2Of disease,with
R3348 T7027 T7026 arg1Of disease,inactive
R3349 T7033 T7032 arg1Of College,American
R3350 T7033 T7034 arg1Of College,of
R3351 T7035 T7034 arg2Of Rheumatology,of
R3352 T7037 T7030 arg2Of criteria,satisfied
R3353 T7037 T7031 arg1Of criteria,the
R3354 T7037 T7033 arg1Of criteria,College
R3355 T7037 T7036 arg1Of criteria,preliminary
R3356 T7037 T7038 arg1Of criteria,for
R3357 T7040 T7038 arg2Of remission,for
R3358 T7040 T7039 arg1Of remission,clinical
R3359 T7040 T7041 arg1Of remission,[
R3360 T7042 T7041 arg2Of 26,[
R3361 T7043 T7041 arg3Of ],[
R3362 T7046 T7044 arg1Of volunteers,Sixteen
R3363 T7046 T7045 arg1Of volunteers,healthy
R3364 T7046 T7047 arg1Of volunteers,(
R3365 T7046 T7061 arg1Of volunteers,served
R3366 T7049 T7048 arg1Of women,11
R3367 T7049 T7050 arg1Of women,and
R3368 T7050 T7047 arg2Of and,(
R3369 T7050 T7053 arg1Of and,;
R3370 T7052 T7050 arg2Of men,and
R3371 T7052 T7051 arg1Of men,five
R3372 T7054 T7053 arg2Of age,;
R3373 T7054 T7055 arg1Of age,","
R3374 T7056 T7057 arg1Of 45.8,±
R3375 T7058 T7057 arg2Of 11.2,±
R3376 T7059 T7055 arg2Of years,","
R3377 T7059 T7056 arg1Of years,45.8
R3378 T7059 T7058 arg1Of years,11.2
R3379 T7060 T7047 arg3Of ),(
R3380 T7061 T7062 arg1Of served,as
R3381 T7063 T7062 arg2Of controls,as
R3382 T7065 T7064 arg1Of samples,ST
R3383 T7065 T7066 arg1Of samples,were
R3384 T7065 T7067 arg2Of samples,obtained
R3385 T7067 T7066 arg2Of obtained,were
R3386 T7067 T7068 arg1Of obtained,from
R3387 T7071 T7068 arg2Of patients,from
R3388 T7071 T7069 arg1Of patients,three
R3389 T7071 T7070 arg1Of patients,RA
R3390 T7071 T7072 arg1Of patients,undergoing
R3391 T7075 T7072 arg2Of replacement,undergoing
R3392 T7075 T7073 arg1Of replacement,total
R3393 T7075 T7074 arg1Of replacement,knee
R3394 T7077 T7076 arg1Of patients,All
R3395 T7077 T7078 arg1Of patients,gave
R3396 T7080 T7078 arg2Of consent,gave
R3397 T7080 T7079 arg1Of consent,informed
R3614 T7511 T7512 arg1Of Isolation,of
R3615 T7515 T7512 arg2Of cells,of
R3616 T7515 T7513 arg1Of cells,CD4+
R3617 T7515 T7514 arg1Of cells,T
R3618 T7519 T7516 arg1Of cells,Peripheral
R3619 T7519 T7517 arg1Of cells,blood
R3620 T7519 T7518 arg1Of cells,mononuclear
R3621 T7519 T7520 arg1Of cells,(
R3622 T7519 T7523 arg1Of cells,were
R3623 T7519 T7524 arg2Of cells,prepared
R3624 T7521 T7520 arg2Of PBMC,(
R3625 T7522 T7520 arg3Of ),(
R3626 T7524 T7523 arg2Of prepared,were
R3627 T7524 T7525 arg1Of prepared,from
R3628 T7528 T7525 arg2Of samples,from
R3629 T7528 T7526 arg2Of samples,heparinized
R3630 T7528 T7527 arg1Of samples,blood
R3631 T7528 T7529 arg1Of samples,by
R3632 T7530 T7529 arg2Of centrifugation,by
R3633 T7530 T7531 arg1Of centrifugation,over
R3634 T7534 T7531 arg2Of gradients,over
R3635 T7534 T7532 arg1Of gradients,Ficoll-Hypaque
R3636 T7534 T7533 arg1Of gradients,density
R3637 T7534 T7535 arg1Of gradients,(
R3638 T7536 T7537 arg1Of Pharmacia,","
R3639 T7537 T7535 arg2Of ",",(
R3640 T7537 T7539 arg1Of ",",","
R3641 T7538 T7537 arg2Of Uppsala,","
R3642 T7540 T7539 arg2Of Sweden,","
R3643 T7541 T7535 arg3Of ),(
R3644 T7544 T7542 arg1Of cells,CD4+
R3645 T7544 T7543 arg1Of cells,T
R3646 T7544 T7545 arg1Of cells,were
R3647 T7544 T7546 arg2Of cells,purified
R3648 T7546 T7545 arg2Of purified,were
R3649 T7546 T7547 arg1Of purified,from
R3650 T7546 T7565 arg1Of purified,","
R3651 T7546 T7566 arg1Of purified,according
R3652 T7548 T7547 arg2Of PBMC,from
R3653 T7551 T7546 arg1Of selection,purified
R3654 T7551 T7549 arg2Of selection,by
R3655 T7551 T7550 arg1Of selection,positive
R3656 T7551 T7552 arg1Of selection,using
R3657 T7555 T7553 arg1Of magnetic,anti-CD4
R3658 T7555 T7554 arg1Of magnetic,mAb-coated
R3659 T7556 T7552 arg2Of beads,using
R3660 T7556 T7555 arg1Of beads,magnetic
R3661 T7556 T7557 arg1Of beads,(
R3662 T7559 T7557 arg2Of Biotec,(
R3663 T7559 T7558 arg1Of Biotec,Miltenyi
R3664 T7559 T7560 arg1Of Biotec,","
R3665 T7561 T7560 arg2Of Gladbach,","
R3666 T7561 T7562 arg1Of Gladbach,","
R3667 T7563 T7562 arg2Of Germany,","
R3668 T7564 T7557 arg3Of ),(
R3669 T7567 T7566 arg2Of to,according
R3670 T7569 T7568 arg1Of manufacturer,the
R3671 T7569 T7570 arg2Of manufacturer,'s
R3672 T7571 T7567 arg2Of instructions,to
R3673 T7571 T7570 arg1Of instructions,'s
R3674 T7574 T7572 arg1Of cells,CD4+
R3675 T7574 T7573 arg1Of cells,T
R3676 T7574 T7575 arg1Of cells,were
R3677 T7574 T7576 arg2Of cells,isolated
R3678 T7576 T7575 arg2Of isolated,were
R3679 T7576 T7577 arg1Of isolated,from
R3680 T7579 T7577 arg2Of samples,from
R3681 T7579 T7578 arg1Of samples,ST
R3682 T7579 T7580 arg1Of samples,","
R3683 T7579 T7581 arg1Of samples,as
R3684 T7583 T7582 arg1Of described,previously
R3685 T7585 T7581 arg2Of 27,as
R3686 T7585 T7583 arg1Of 27,described
R3687 T7585 T7584 arg2Of 27,[
R3688 T7586 T7584 arg3Of ],[
R3689 T7591 T7589 arg1Of samples,fresh
R3690 T7591 T7590 arg1Of samples,ST
R3691 T7591 T7592 arg1Of samples,were
R3692 T7591 T7593 arg2Of samples,fragmented
R3693 T7591 T7595 arg2Of samples,digested
R3694 T7591 T7606 arg1Of samples,removing
R3695 T7593 T7594 arg1Of fragmented,and
R3696 T7594 T7587 arg1Of and,Briefly
R3697 T7594 T7588 arg1Of and,","
R3698 T7594 T7592 arg2Of and,were
R3699 T7594 T7605 arg1Of and,After
R3700 T7595 T7594 arg2Of digested,and
R3701 T7595 T7596 arg1Of digested,with
R3702 T7595 T7600 arg1Of digested,for
R3703 T7597 T7598 arg1Of collagenase,and
R3704 T7598 T7596 arg2Of and,with
R3705 T7599 T7598 arg2Of DNase,and
R3706 T7602 T7600 arg2Of hour,for
R3707 T7602 T7601 arg1Of hour,1
R3708 T7602 T7603 arg1Of hour,at
R3709 T7604 T7603 arg2Of 37°C.,at
R3710 T7606 T7605 arg2Of removing,After
R3711 T7608 T7606 arg2Of debris,removing
R3712 T7608 T7607 arg1Of debris,tissue
R3713 T7608 T7667 modOf debris,were
R3714 T7612 T7610 arg1Of suspensions,ST
R3715 T7612 T7611 arg1Of suspensions,cell
R3716 T7612 T7613 arg1Of suspensions,in
R3717 T7612 T7616 arg1Of suspensions,(
R3718 T7612 T7630 arg2Of suspensions,supplemented
R3719 T7612 T7658 arg1Of suspensions,with
R3720 T7612 T7667 arg1Of suspensions,were
R3721 T7612 T7668 arg2Of suspensions,incubated
R3722 T7615 T7613 arg2Of medium,in
R3723 T7615 T7614 arg1Of medium,culture
R3724 T7619 T7617 arg1Of medium,RPMI
R3725 T7619 T7618 arg1Of medium,1640
R3726 T7619 T7620 arg1Of medium,;
R3727 T7620 T7616 arg2Of ;,(
R3728 T7620 T7623 arg1Of ;,","
R3729 T7620 T7627 arg1Of ;,","
R3730 T7620 T7628 arg1Of ;,USA
R3731 T7622 T7620 arg2Of Technologies,;
R3732 T7622 T7621 arg1Of Technologies,Life
R3733 T7624 T7623 arg2Of Gaithersburg,","
R3734 T7624 T7625 arg1Of Gaithersburg,","
R3735 T7626 T7625 arg2Of MD,","
R3736 T7629 T7616 arg3Of ),(
R3737 T7630 T7631 arg1Of supplemented,with
R3738 T7632 T7633 arg1Of 25,mM
R3739 T7634 T7631 arg2Of HEPES,with
R3740 T7634 T7632 arg1Of HEPES,25
R3741 T7634 T7635 arg1Of HEPES,(
R3742 T7638 T7636 arg1Of L-glutamine,2
R3743 T7638 T7637 arg1Of L-glutamine,mM
R3744 T7638 T7639 arg1Of L-glutamine,","
R3745 T7639 T7645 arg1Of ",",","
R3746 T7640 T7641 arg1Of 2,%
R3747 T7644 T7639 arg2Of acids,","
R3748 T7644 T7640 arg1Of acids,2
R3749 T7644 T7642 arg1Of acids,nonessential
R3750 T7644 T7643 arg1Of acids,amino
R3751 T7645 T7650 arg1Of ",",and
R3752 T7648 T7645 arg2Of penicillin,","
R3753 T7648 T7646 arg1Of penicillin,100
R3754 T7648 T7647 arg1Of penicillin,IU/ml
R3755 T7650 T7635 arg2Of and,(
R3756 T7650 T7649 arg1Of and,","
R3757 T7653 T7651 arg1Of streptomycin,100
R3758 T7653 T7652 arg1Of streptomycin,mg/ml
R3759 T7653 T7654 arg1Of streptomycin,;
R3760 T7654 T7650 arg2Of ;,and
R3761 T7656 T7654 arg2Of Technologies,;
R3762 T7656 T7655 arg1Of Technologies,Life
R3763 T7657 T7635 arg3Of ),(
R3764 T7659 T7660 arg1Of 10,%
R3765 T7662 T7658 arg2Of FCS,with
R3766 T7662 T7659 arg1Of FCS,10
R3767 T7662 T7661 arg1Of FCS,heat-inactivated
R3768 T7662 T7663 arg1Of FCS,(
R3769 T7665 T7663 arg2Of Technologies,(
R3770 T7665 T7664 arg1Of Technologies,Life
R3771 T7666 T7663 arg3Of ),(
R3772 T7668 T7609 arg1Of incubated,","
R3773 T7668 T7667 arg2Of incubated,were
R3774 T7668 T7669 arg1Of incubated,at
R3775 T7670 T7669 arg2Of 37°C,at
R3776 T7670 T7671 arg1Of 37°C,in
R3777 T7673 T7671 arg2Of plates,in
R3778 T7673 T7672 arg1Of plates,six-well
R3779 T7673 T7674 arg1Of plates,(
R3780 T7673 T7683 arg1Of plates,for
R3781 T7675 T7676 arg1Of Coster,","
R3782 T7676 T7678 arg1Of ",",","
R3783 T7677 T7676 arg2Of Cambridge,","
R3784 T7678 T7674 arg2Of ",",(
R3785 T7678 T7680 arg1Of ",",","
R3786 T7678 T7681 arg1Of ",",USA
R3787 T7679 T7678 arg2Of MA,","
R3788 T7682 T7674 arg3Of ),(
R3789 T7685 T7683 arg2Of min,for
R3790 T7685 T7684 arg1Of min,45
R3791 T7687 T7686 arg1Of cells,Non-adherent
R3792 T7687 T7688 arg1Of cells,were
R3793 T7687 T7689 arg2Of cells,harvested
R3794 T7689 T7688 arg2Of harvested,were
R3795 T7689 T7690 arg1Of harvested,and
R3796 T7693 T7691 arg1Of cells,CD4+
R3797 T7693 T7692 arg1Of cells,T
R3798 T7693 T7694 arg1Of cells,were
R3799 T7693 T7695 arg2Of cells,purified
R3800 T7695 T7690 arg2Of purified,and
R3801 T7695 T7694 arg2Of purified,were
R3802 T7698 T7695 arg1Of selection,purified
R3803 T7698 T7696 arg2Of selection,by
R3804 T7698 T7697 arg1Of selection,positive
R3805 T7698 T7699 arg1Of selection,as
R3806 T7701 T7699 arg2Of described,as
R3807 T7701 T7700 arg1Of described,already
R4017 T8108 T8109 arg1Of Culture,of
R4018 T8112 T8109 arg2Of cells,of
R4019 T8112 T8110 arg1Of cells,CD4+
R4020 T8112 T8111 arg1Of cells,T
R4021 T8116 T8113 arg1Of populations,PB
R4022 T8116 T8114 arg1Of populations,CD4+
R4023 T8116 T8115 arg1Of populations,T-cell
R4024 T8116 T8117 arg1Of populations,were
R4025 T8116 T8118 arg2Of populations,resuspended
R4026 T8118 T8117 arg2Of resuspended,were
R4027 T8118 T8119 arg1Of resuspended,at
R4028 T8118 T8135 arg1Of resuspended,and
R4029 T8121 T8119 arg2Of density,at
R4030 T8121 T8120 arg1Of density,a
R4031 T8121 T8122 arg1Of density,of
R4032 T8121 T8127 arg1Of density,in
R4033 T8126 T8122 arg2Of cells/ml,of
R4034 T8126 T8123 arg1Of cells/ml,1
R4035 T8126 T8124 arg1Of cells/ml,×
R4036 T8126 T8125 arg1Of cells/ml,106
R4037 T8129 T8127 arg2Of medium,in
R4038 T8129 T8128 arg1Of medium,culture
R4039 T8129 T8130 arg1Of medium,with
R4040 T8131 T8132 arg1Of 10,%
R4041 T8133 T8130 arg2Of FCS,with
R4042 T8133 T8131 arg1Of FCS,10
R4043 T8135 T8134 arg1Of and,","
R4044 T8139 T8136 arg1Of suspensions,0.5
R4045 T8139 T8137 arg1Of suspensions,ml
R4046 T8139 T8138 arg1Of suspensions,cell
R4047 T8139 T8140 arg1Of suspensions,were
R4048 T8139 T8141 arg2Of suspensions,dispensed
R4049 T8141 T8135 arg2Of dispensed,and
R4050 T8141 T8140 arg2Of dispensed,were
R4051 T8141 T8142 arg1Of dispensed,into
R4052 T8141 T8158 arg1Of dispensed,(
R4053 T8144 T8142 arg2Of wells,into
R4054 T8144 T8143 arg1Of wells,the
R4055 T8144 T8145 arg1Of wells,of
R4056 T8144 T8152 arg2Of wells,coated
R4057 T8148 T8145 arg2Of plates,of
R4058 T8148 T8146 arg1Of plates,24-well
R4059 T8148 T8147 arg1Of plates,microtiter
R4060 T8148 T8149 arg1Of plates,(
R4061 T8150 T8149 arg2Of Coster,(
R4062 T8151 T8149 arg3Of ),(
R4063 T8152 T8153 arg1Of coated,with
R4064 T8157 T8153 arg2Of mAb,with
R4065 T8157 T8154 arg1Of mAb,1
R4066 T8157 T8155 arg1Of mAb,μg/ml
R4067 T8157 T8156 arg1Of mAb,anti-CD3
R4068 T8159 T8160 arg1Of Immunotech,","
R4069 T8160 T8162 arg1Of ",",","
R4070 T8161 T8160 arg2Of Marseille,","
R4071 T8162 T8158 arg2Of ",",(
R4072 T8163 T8162 arg2Of France,","
R4073 T8164 T8158 arg3Of ),(
R4074 T8166 T8165 arg1Of cells,The
R4075 T8166 T8167 arg1Of cells,were
R4076 T8166 T8168 arg2Of cells,incubated
R4077 T8168 T8167 arg2Of incubated,were
R4078 T8168 T8169 arg1Of incubated,with
R4079 T8168 T8177 arg1Of incubated,in
R4080 T8173 T8169 arg2Of mAb,with
R4081 T8173 T8170 arg1Of mAb,1
R4082 T8173 T8171 arg1Of mAb,μg/ml
R4083 T8173 T8172 arg1Of mAb,anti-CD28
R4084 T8173 T8174 arg1Of mAb,(
R4085 T8175 T8174 arg2Of Immunotech,(
R4086 T8176 T8174 arg3Of ),(
R4087 T8179 T8180 arg1Of presence,or
R4088 T8180 T8177 arg2Of or,in
R4089 T8180 T8178 arg1Of or,the
R4090 T8180 T8182 arg1Of or,of
R4091 T8180 T8199 arg1Of or,at
R4092 T8180 T8201 arg1Of or,in
R4093 T8181 T8180 arg2Of absence,or
R4094 T8185 T8182 arg2Of concentrations,of
R4095 T8185 T8183 arg1Of concentrations,the
R4096 T8185 T8184 arg2Of concentrations,indicated
R4097 T8185 T8186 arg1Of concentrations,of
R4098 T8187 T8186 arg2Of IL-10,of
R4099 T8187 T8188 arg1Of IL-10,(
R4100 T8190 T8188 arg2Of Dickinson,(
R4101 T8190 T8189 arg1Of Dickinson,Becton
R4102 T8190 T8191 arg1Of Dickinson,","
R4103 T8190 T8196 arg1Of Dickinson,","
R4104 T8190 T8197 arg1Of Dickinson,USA
R4105 T8193 T8191 arg2Of Jose,","
R4106 T8193 T8192 arg1Of Jose,San
R4107 T8193 T8194 arg1Of Jose,","
R4108 T8195 T8194 arg2Of CA,","
R4109 T8198 T8188 arg3Of ),(
R4110 T8200 T8199 arg2Of 37°C,at
R4111 T8204 T8201 arg2Of atmosphere,in
R4112 T8204 T8202 arg1Of atmosphere,a
R4113 T8204 T8203 arg1Of atmosphere,humidified
R4114 T8204 T8205 arg1Of atmosphere,containing
R4115 T8206 T8207 arg1Of 5,%
R4116 T8208 T8205 arg2Of CO2,containing
R4117 T8208 T8206 arg1Of CO2,5
R4118 T8208 T8209 arg1Of CO2,[
R4119 T8210 T8209 arg2Of 28,[
R4120 T8211 T8209 arg3Of ],[
R4121 T8213 T8212 arg1Of supernatants,Culture
R4122 T8213 T8214 arg1Of supernatants,were
R4123 T8213 T8215 arg2Of supernatants,collected
R4124 T8215 T8214 arg2Of collected,were
R4125 T8215 T8218 arg1Of collected,later
R4126 T8215 T8219 arg1Of collected,and
R4127 T8217 T8216 arg1Of hours,36
R4128 T8218 T8217 arg1Of later,hours
R4129 T8221 T8220 arg1Of samples,cell-free
R4130 T8221 T8222 arg1Of samples,were
R4131 T8221 T8223 arg2Of samples,stored
R4132 T8223 T8219 arg2Of stored,and
R4133 T8223 T8222 arg2Of stored,were
R4134 T8223 T8224 arg1Of stored,at
R4135 T8223 T8226 arg1Of stored,until
R4136 T8225 T8224 arg2Of -30°C,at
R4137 T8228 T8226 arg2Of assay,until
R4138 T8228 T8227 arg1Of assay,cytokine
R4139 T8230 T8229 arg1Of examine,To
R4140 T8232 T8230 arg2Of effect,examine
R4141 T8232 T8231 arg1Of effect,the
R4142 T8232 T8233 arg1Of effect,of
R4143 T8232 T8235 arg1Of effect,on
R4144 T8234 T8233 arg2Of IL-6,of
R4145 T8237 T8235 arg2Of responsiveness,on
R4146 T8237 T8236 arg1Of responsiveness,T-cell
R4147 T8237 T8238 arg1Of responsiveness,to
R4148 T8239 T8238 arg2Of IL-10,to
R4149 T8243 T8241 arg1Of cells,CD4+
R4150 T8243 T8242 arg1Of cells,T
R4151 T8243 T8244 arg1Of cells,from
R4152 T8243 T8247 arg1Of cells,were
R4153 T8243 T8248 arg2Of cells,incubated
R4154 T8246 T8244 arg2Of controls,from
R4155 T8246 T8245 arg1Of controls,healthy
R4156 T8248 T8229 modOf incubated,To
R4157 T8248 T8240 arg1Of incubated,","
R4158 T8248 T8247 arg2Of incubated,were
R4159 T8248 T8249 arg1Of incubated,in
R4160 T8248 T8256 arg1Of incubated,in
R4161 T8251 T8249 arg2Of medium,in
R4162 T8251 T8250 arg1Of medium,culture
R4163 T8251 T8252 arg1Of medium,with
R4164 T8253 T8254 arg1Of 10,%
R4165 T8255 T8252 arg2Of FCS,with
R4166 T8255 T8253 arg1Of FCS,10
R4167 T8258 T8259 arg1Of presence,or
R4168 T8259 T8256 arg2Of or,in
R4169 T8259 T8257 arg1Of or,the
R4170 T8259 T8261 arg1Of or,of
R4171 T8260 T8259 arg2Of absence,or
R4172 T8262 T8263 arg1Of 10,ng/ml
R4173 T8264 T8261 arg2Of IL-6,of
R4174 T8264 T8262 arg1Of IL-6,10
R4175 T8264 T8265 arg1Of IL-6,(
R4176 T8264 T8269 arg1Of IL-6,for
R4177 T8267 T8265 arg2Of Dickinson,(
R4178 T8267 T8266 arg1Of Dickinson,Becton
R4179 T8268 T8265 arg3Of ),(
R4180 T8271 T8269 arg2Of hours,for
R4181 T8271 T8270 arg1Of hours,36
R4182 T8272 T8273 arg1Of Cells,were
R4183 T8272 T8275 arg2Of Cells,stimulated
R4184 T8275 T8273 arg2Of stimulated,were
R4185 T8275 T8274 arg1Of stimulated,then
R4186 T8275 T8276 arg1Of stimulated,for
R4187 T8275 T8285 arg1Of stimulated,in
R4188 T8278 T8276 arg2Of hours,for
R4189 T8278 T8277 arg1Of hours,36
R4190 T8278 T8279 arg1Of hours,with
R4191 T8281 T8280 arg1Of mAb,anti-CD3
R4192 T8281 T8282 arg1Of mAb,and
R4193 T8282 T8279 arg2Of and,with
R4194 T8284 T8282 arg2Of mAb,and
R4195 T8284 T8283 arg1Of mAb,anti-CD28
R4196 T8287 T8288 arg1Of presence,or
R4197 T8288 T8285 arg2Of or,in
R4198 T8288 T8286 arg1Of or,the
R4199 T8288 T8290 arg1Of or,of
R4200 T8289 T8288 arg2Of absence,or
R4201 T8291 T8292 arg1Of 1,ng/ml
R4202 T8293 T8290 arg2Of IL-10,of
R4203 T8293 T8291 arg1Of IL-10,1
R4204 T8295 T8294 arg1Of supernatants,Culture
R4205 T8295 T8296 arg1Of supernatants,were
R4206 T8295 T8297 arg2Of supernatants,measured
R4207 T8297 T8296 arg2Of measured,were
R4208 T8297 T8298 arg1Of measured,for
R4209 T8300 T8298 arg2Of concentrations,for
R4210 T8300 T8299 arg1Of concentrations,IFN-γ
R4426 T8725 T8723 arg1Of analysis,Flow
R4427 T8725 T8724 arg1Of analysis,cytometric
R4428 T8725 T8726 arg1Of analysis,for
R4429 T8728 T8726 arg2Of expression,for
R4430 T8728 T8727 arg1Of expression,IL-10R1
R4431 T8730 T8729 arg1Of sample,A
R4432 T8730 T8731 arg1Of sample,of
R4433 T8730 T8738 arg1Of sample,was
R4434 T8730 T8739 arg2Of sample,resuspended
R4435 T8734 T8733 arg1Of 105,×
R4436 T8735 T8731 arg2Of cells,of
R4437 T8735 T8732 arg1Of cells,5
R4438 T8735 T8734 arg1Of cells,105
R4439 T8735 T8736 arg1Of cells,of
R4440 T8737 T8736 arg2Of PBMC,of
R4441 T8739 T8738 arg2Of resuspended,was
R4442 T8739 T8740 arg1Of resuspended,in
R4443 T8739 T8742 arg1Of resuspended,with
R4444 T8741 T8740 arg2Of PBS,in
R4445 T8743 T8744 arg1Of 1,%
R4446 T8745 T8742 arg2Of FCS,with
R4447 T8745 T8743 arg1Of FCS,1
R4448 T8746 T8747 arg1Of PBMC,were
R4449 T8746 T8748 arg2Of PBMC,incubated
R4450 T8748 T8747 arg2Of incubated,were
R4451 T8748 T8749 arg1Of incubated,with
R4452 T8748 T8770 arg1Of incubated,with
R4453 T8748 T8777 arg1Of incubated,","
R4454 T8748 T8778 modOf incubated,followed
R4455 T8749 T8769 arg1Of with,or
R4456 T8751 T8749 arg2Of concentrations,with
R4457 T8751 T8750 arg1Of concentrations,saturating
R4458 T8751 T8752 arg1Of concentrations,of
R4459 T8751 T8755 arg1Of concentrations,(
R4460 T8754 T8752 arg2Of mAb,of
R4461 T8754 T8753 arg1Of mAb,anti-IL-10R1
R4462 T8756 T8755 arg2Of IgG1,(
R4463 T8756 T8757 arg1Of IgG1,;
R4464 T8756 T8766 arg1Of IgG1,","
R4465 T8756 T8767 arg1Of IgG1,USA
R4466 T8761 T8757 arg2Of systems,;
R4467 T8761 T8758 arg1Of systems,R
R4468 T8761 T8759 arg1Of systems,&
R4469 T8761 T8760 arg1Of systems,D
R4470 T8761 T8762 arg1Of systems,","
R4471 T8763 T8764 arg1Of Minneapolis,","
R4472 T8764 T8762 arg2Of ",",","
R4473 T8765 T8764 arg2Of MN,","
R4474 T8768 T8755 arg3Of ),(
R4475 T8770 T8769 arg2Of with,or
R4476 T8773 T8770 arg2Of mAb,with
R4477 T8773 T8771 arg1Of mAb,isotype-matched
R4478 T8773 T8772 arg1Of mAb,control
R4479 T8773 T8774 arg1Of mAb,(
R4480 T8775 T8774 arg2Of Immunotech,(
R4481 T8776 T8774 arg3Of ),(
R4482 T8780 T8778 arg1Of incubation,followed
R4483 T8780 T8779 arg2Of incubation,by
R4484 T8780 T8781 arg1Of incubation,with
R4485 T8787 T8781 arg2Of antibody,with
R4486 T8787 T8782 arg1Of antibody,FITC-conjugated
R4487 T8787 T8783 arg1Of antibody,goat
R4488 T8787 T8784 arg1Of antibody,anti-mouse
R4489 T8787 T8785 arg1Of antibody,IgG1
R4490 T8787 T8786 arg1Of antibody,polyclonal
R4491 T8787 T8788 arg1Of antibody,(
R4492 T8791 T8788 arg2Of Biotechnologies,(
R4493 T8791 T8789 arg1Of Biotechnologies,Santa
R4494 T8791 T8790 arg1Of Biotechnologies,Cruz
R4495 T8791 T8792 arg1Of Biotechnologies,","
R4496 T8791 T8797 arg1Of Biotechnologies,","
R4497 T8791 T8798 arg1Of Biotechnologies,USA
R4498 T8794 T8792 arg2Of Cruz,","
R4499 T8794 T8793 arg1Of Cruz,Santa
R4500 T8794 T8795 arg1Of Cruz,","
R4501 T8796 T8795 arg2Of CA,","
R4502 T8799 T8788 arg3Of ),(
R4503 T8800 T8801 arg1Of Cells,were
R4504 T8800 T8803 arg2Of Cells,incubated
R4505 T8803 T8801 arg2Of incubated,were
R4506 T8803 T8802 arg1Of incubated,then
R4507 T8803 T8804 arg1Of incubated,with
R4508 T8807 T8804 arg2Of mAb,with
R4509 T8807 T8805 arg1Of mAb,phycoerythrin-conjugated
R4510 T8807 T8806 arg1Of mAb,anti-CD4
R4511 T8807 T8808 arg1Of mAb,(
R4512 T8810 T8808 arg2Of Dickinson,(
R4513 T8810 T8809 arg1Of Dickinson,Becton
R4514 T8811 T8808 arg3Of ),(
R4515 T8812 T8813 arg1Of Cells,were
R4516 T8812 T8814 arg2Of Cells,washed
R4517 T8814 T8813 arg2Of washed,were
R4518 T8814 T8815 arg1Of washed,well
R4519 T8814 T8816 arg1Of washed,with
R4520 T8817 T8818 arg1Of 1,%
R4521 T8819 T8816 arg2Of FCS/PBS,with
R4522 T8819 T8817 arg1Of FCS/PBS,1
R4523 T8819 T8820 arg1Of FCS/PBS,between
R4524 T8821 T8820 arg2Of incubations,between
R4525 T8822 T8823 arg1Of Analysis,was
R4526 T8822 T8824 arg2Of Analysis,performed
R4527 T8824 T8823 arg2Of performed,was
R4528 T8824 T8825 arg1Of performed,on
R4529 T8829 T8825 arg2Of cytometer,on
R4530 T8829 T8826 arg1Of cytometer,a
R4531 T8829 T8827 arg1Of cytometer,FACScan
R4532 T8829 T8828 arg1Of cytometer,flow
R4533 T8829 T8830 arg1Of cytometer,(
R4534 T8832 T8830 arg2Of Dickinson,(
R4535 T8832 T8831 arg1Of Dickinson,Becton
R4536 T8833 T8830 arg3Of ),(
R4657 T9111 T9112 arg1Of Immunoassay,for
R4658 T9113 T9114 arg1Of IFN-γ,and
R4659 T9114 T9112 arg2Of and,for
R4660 T9115 T9114 arg2Of IL-2,and
R4661 T9116 T9117 arg1Of Concentrations,of
R4662 T9116 T9121 arg1Of Concentrations,in
R4663 T9116 T9128 arg1Of Concentrations,were
R4664 T9116 T9129 arg2Of Concentrations,measured
R4665 T9118 T9119 arg1Of IFN-γ,and
R4666 T9119 T9117 arg2Of and,of
R4667 T9120 T9119 arg2Of IL-2,and
R4668 T9123 T9121 arg2Of supernatants,in
R4669 T9123 T9122 arg1Of supernatants,culture
R4670 T9123 T9124 arg1Of supernatants,of
R4671 T9127 T9124 arg2Of cells,of
R4672 T9127 T9125 arg1Of cells,CD4+
R4673 T9127 T9126 arg1Of cells,T
R4674 T9129 T9128 arg2Of measured,were
R4675 T9129 T9130 arg1Of measured,in
R4676 T9129 T9132 arg1Of measured,by
R4677 T9131 T9130 arg2Of duplicate,in
R4678 T9136 T9132 arg2Of ELISA,by
R4679 T9136 T9133 arg1Of ELISA,the
R4680 T9136 T9134 arg1Of ELISA,quantitative
R4681 T9136 T9135 arg1Of ELISA,sandwich
R4682 T9136 T9137 arg1Of ELISA,using
R4683 T9137 T9140 arg1Of using,with
R4684 T9137 T9154 arg1Of using,","
R4685 T9137 T9155 arg1Of using,according
R4686 T9139 T9137 arg2Of capture,using
R4687 T9139 T9138 arg1Of capture,cytokine-specific
R4688 T9143 T9141 arg2Of mAb,biotinylated
R4689 T9143 T9142 arg1Of mAb,detection
R4690 T9143 T9144 arg1Of mAb,and
R4691 T9144 T9140 arg2Of and,with
R4692 T9147 T9144 arg2Of proteins,and
R4693 T9147 T9145 arg1Of proteins,recombinant
R4694 T9147 T9146 arg1Of proteins,cytokine
R4695 T9147 T9148 arg1Of proteins,(
R4696 T9149 T9148 arg2Of all,(
R4697 T9149 T9150 arg1Of all,from
R4698 T9152 T9150 arg2Of Dickinson,from
R4699 T9152 T9151 arg1Of Dickinson,Becton
R4700 T9153 T9148 arg3Of ),(
R4701 T9156 T9155 arg2Of to,according
R4702 T9158 T9157 arg1Of manufacturer,the
R4703 T9158 T9159 arg2Of manufacturer,'s
R4704 T9160 T9156 arg2Of protocol,to
R4705 T9160 T9159 arg1Of protocol,'s
R4706 T9163 T9161 arg1Of limits,The
R4707 T9163 T9162 arg1Of limits,detection
R4708 T9163 T9164 arg1Of limits,for
R4709 T9163 T9168 arg1Of limits,were
R4710 T9165 T9166 arg1Of IFN-γ,and
R4711 T9166 T9164 arg2Of and,for
R4712 T9167 T9166 arg2Of IL-2,and
R4713 T9170 T9168 arg2Of pg/ml,were
R4714 T9170 T9169 arg1Of pg/ml,15
R4791 T9809 T9810 arg1Of Isolation,of
R4792 T9811 T9812 arg1Of mRNA,and
R4793 T9812 T9810 arg2Of and,of
R4794 T9814 T9812 arg2Of PCR,and
R4795 T9814 T9813 arg1Of PCR,real-time
R4796 T9817 T9815 arg1Of RNA,Total
R4797 T9817 T9816 arg1Of RNA,cellular
R4798 T9817 T9818 arg1Of RNA,was
R4799 T9817 T9819 arg2Of RNA,extracted
R4800 T9819 T9818 arg2Of extracted,was
R4801 T9819 T9820 arg1Of extracted,from
R4802 T9824 T9820 arg2Of cells,from
R4803 T9824 T9821 arg1Of cells,PB
R4804 T9824 T9822 arg1Of cells,CD4+
R4805 T9824 T9823 arg1Of cells,T
R4806 T9824 T9825 arg1Of cells,using
R4807 T9824 T9851 modOf cells,was
R4808 T9825 T9830 arg1Of using,(
R4809 T9825 T9843 arg1Of using,","
R4810 T9825 T9844 arg1Of using,according
R4811 T9829 T9825 arg2Of kit,using
R4812 T9829 T9826 arg1Of kit,an
R4813 T9829 T9827 arg1Of kit,RNA
R4814 T9829 T9828 arg1Of kit,isolation
R4815 T9832 T9831 arg1Of Mini,RNeasy
R4816 T9833 T9832 arg1Of kit,Mini
R4817 T9833 T9834 arg1Of kit,;
R4818 T9834 T9830 arg2Of ;,(
R4819 T9834 T9840 arg1Of ;,","
R4820 T9834 T9841 arg1Of ;,USA
R4821 T9835 T9836 arg1Of Qiagen,","
R4822 T9836 T9838 arg1Of ",",","
R4823 T9837 T9836 arg2Of Valencia,","
R4824 T9838 T9834 arg2Of ",",;
R4825 T9839 T9838 arg2Of CA,","
R4826 T9842 T9830 arg3Of ),(
R4827 T9845 T9844 arg2Of to,according
R4828 T9848 T9845 arg2Of 's,to
R4829 T9848 T9846 arg1Of 's,the
R4830 T9848 T9847 arg1Of 's,manufacturer
R4831 T9848 T9849 arg1Of 's,instructions.
R4832 T9850 T9851 arg1Of cDNA,was
R4833 T9850 T9852 arg2Of cDNA,synthesized
R4834 T9852 T9851 arg2Of synthesized,was
R4835 T9852 T9853 arg1Of synthesized,from
R4836 T9852 T9856 arg1Of synthesized,with
R4837 T9855 T9853 arg2Of RNA,from
R4838 T9855 T9854 arg1Of RNA,total
R4839 T9862 T9857 arg1Of transcriptase,Molony
R4840 T9862 T9858 arg1Of transcriptase,murine
R4841 T9862 T9859 arg1Of transcriptase,leukemia
R4842 T9862 T9860 arg1Of transcriptase,virus
R4843 T9862 T9861 arg1Of transcriptase,reverse
R4844 T9862 T9863 arg1Of transcriptase,(
R4845 T9862 T9873 arg1Of transcriptase,and
R4846 T9865 T9864 arg1Of Biochemical,US
R4847 T9865 T9866 arg1Of Biochemical,","
R4848 T9866 T9868 arg1Of ",",","
R4849 T9867 T9866 arg2Of Cleveland,","
R4850 T9868 T9863 arg2Of ",",(
R4851 T9868 T9870 arg1Of ",",","
R4852 T9868 T9871 arg1Of ",",USA
R4853 T9869 T9868 arg2Of OH,","
R4854 T9872 T9863 arg3Of ),(
R4855 T9873 T9856 arg2Of and,with
R4856 T9876 T9875 arg2Of dT,(
R4857 T9877 T9875 arg3Of ),(
R4858 T9879 T9873 arg2Of primers,and
R4859 T9879 T9874 arg1Of primers,oligo-
R4860 T9879 T9875 arg1Of primers,(
R4861 T9879 T9878 arg1Of primers,15
R4862 T9879 T9880 arg1Of primers,(
R4863 T9881 T9882 arg1Of Promega,","
R4864 T9882 T9884 arg1Of ",",","
R4865 T9883 T9882 arg2Of Madison,","
R4866 T9884 T9880 arg2Of ",",(
R4867 T9884 T9886 arg1Of ",",","
R4868 T9884 T9887 arg1Of ",",USA
R4869 T9885 T9884 arg2Of WI,","
R4870 T9888 T9880 arg3Of ),(
R4871 T9890 T9889 arg1Of PCR,Real-time
R4872 T9890 T9891 arg1Of PCR,was
R4873 T9890 T9892 arg2Of PCR,performed
R4874 T9892 T9891 arg2Of performed,was
R4875 T9892 T9893 arg1Of performed,with
R4876 T9896 T9893 arg2Of Instrument,with
R4877 T9896 T9894 arg1Of Instrument,the
R4878 T9896 T9895 arg1Of Instrument,LightCycler
R4879 T9896 T9897 arg1Of Instrument,(
R4880 T9896 T9905 arg1Of Instrument,in
R4881 T9899 T9897 arg2Of Diagnostics,(
R4882 T9899 T9898 arg1Of Diagnostics,Roche
R4883 T9899 T9900 arg1Of Diagnostics,","
R4884 T9901 T9900 arg2Of Penzberg,","
R4885 T9901 T9902 arg1Of Penzberg,","
R4886 T9903 T9902 arg2Of Germany,","
R4887 T9904 T9897 arg3Of ),(
R4888 T9907 T9905 arg2Of capillaries,in
R4889 T9907 T9906 arg1Of capillaries,glass
R4890 T9910 T9908 arg1Of mix,The
R4891 T9910 T9909 arg1Of mix,reaction
R4892 T9910 T9911 arg1Of mix,containing
R4893 T9910 T9937 arg1Of mix,were
R4894 T9910 T9938 arg2Of mix,added
R4895 T9914 T9912 arg1Of polymerase,Taq
R4896 T9914 T9913 arg1Of polymerase,DNA
R4897 T9914 T9915 arg1Of polymerase,and
R4898 T9915 T9934 arg1Of and,and
R4899 T9919 T9920 arg1Of Green,I
R4900 T9921 T9915 arg2Of dye,and
R4901 T9921 T9916 arg1Of dye,DNA
R4902 T9921 T9917 arg1Of dye,double-strand-specific
R4903 T9921 T9918 arg1Of dye,SYBR
R4904 T9921 T9919 arg1Of dye,Green
R4905 T9921 T9922 arg1Of dye,(
R4906 T9924 T9923 arg1Of FastStart,Lightcycler
R4907 T9929 T9925 arg1Of I,DNA
R4908 T9929 T9926 arg1Of I,Master
R4909 T9929 T9927 arg1Of I,SYBR
R4910 T9929 T9928 arg1Of I,Green
R4911 T9929 T9930 arg1Of I,;
R4912 T9930 T9922 arg2Of ;,(
R4913 T9930 T9924 arg1Of ;,FastStart
R4914 T9932 T9930 arg2Of Diagnostics,;
R4915 T9932 T9931 arg1Of Diagnostics,Roche
R4916 T9933 T9922 arg3Of ),(
R4917 T9934 T9911 arg2Of and,containing
R4918 T9936 T9934 arg2Of primers,and
R4919 T9936 T9935 arg1Of primers,specific
R4920 T9938 T9937 arg2Of added,were
R4921 T9938 T9939 arg1Of added,to
R4922 T9941 T9939 arg2Of dilutions,to
R4923 T9941 T9940 arg1Of dilutions,cDNA
R4924 T9944 T9942 arg1Of samples,The
R4925 T9944 T9943 arg1Of samples,cDNA
R4926 T9944 T9945 arg1Of samples,were
R4927 T9944 T9946 arg2Of samples,denatured
R4928 T9944 T9955 arg1Of samples,were
R4929 T9944 T9957 arg2Of samples,amplified
R4930 T9946 T9945 arg2Of denatured,were
R4931 T9946 T9947 arg1Of denatured,at
R4932 T9946 T9950 arg1Of denatured,for
R4933 T9946 T9954 arg1Of denatured,and
R4934 T9949 T9947 arg2Of C,at
R4935 T9949 T9948 arg1Of C,95°
R4936 T9952 T9950 arg2Of min,for
R4937 T9952 T9951 arg1Of min,10
R4938 T9954 T9953 arg1Of and,","
R4939 T9957 T9954 arg2Of amplified,and
R4940 T9957 T9955 arg2Of amplified,were
R4941 T9957 T9956 arg1Of amplified,then
R4942 T9957 T9958 arg1Of amplified,for
R4943 T9957 T9961 arg1Of amplified,:
R4944 T9957 T9987 arg1Of amplified,;
R4945 T9957 T9995 arg1Of amplified,","
R4946 T9957 T9996 arg1Of amplified,at
R4947 T9957 T10005 arg1Of amplified,at
R4948 T9957 T10016 arg1Of amplified,at
R4949 T9957 T10033 arg1Of amplified,at
R4950 T9960 T9958 arg2Of cycles,for
R4951 T9960 T9959 arg1Of cycles,40–50
R4952 T9961 T9962 arg1Of :,at
R4953 T9964 T9963 arg1Of C,95°
R4954 T9964 T9965 arg1Of C,(
R4955 T9964 T9969 arg1Of C,","
R4956 T9964 T9970 arg1Of C,at
R4957 T9964 T9978 arg1Of C,and
R4958 T9967 T9965 arg2Of s,(
R4959 T9967 T9966 arg1Of s,10
R4960 T9968 T9965 arg3Of ),(
R4961 T9972 T9970 arg2Of C,at
R4962 T9972 T9971 arg1Of C,65°
R4963 T9972 T9973 arg1Of C,(
R4964 T9975 T9973 arg2Of s,(
R4965 T9975 T9974 arg1Of s,15
R4966 T9976 T9973 arg3Of ),(
R4967 T9978 T9962 arg2Of and,at
R4968 T9978 T9977 arg1Of and,","
R4969 T9980 T9978 arg2Of C,and
R4970 T9980 T9979 arg1Of C,72°
R4971 T9980 T9981 arg1Of C,(
R4972 T9980 T9985 arg1Of C,for
R4973 T9983 T9981 arg2Of s,(
R4974 T9983 T9982 arg1Of s,22
R4975 T9984 T9981 arg3Of ),(
R4976 T9986 T9985 arg2Of β-actin,for
R4977 T9987 T9988 arg1Of ;,at
R4978 T9990 T9988 arg2Of C,at
R4979 T9990 T9989 arg1Of C,95°
R4980 T9990 T9991 arg1Of C,(
R4981 T9993 T9991 arg2Of s,(
R4982 T9993 T9992 arg1Of s,10
R4983 T9994 T9991 arg3Of ),(
R4984 T9996 T10004 arg1Of at,and
R4985 T9998 T9996 arg2Of C,at
R4986 T9998 T9997 arg1Of C,62°
R4987 T9998 T9999 arg1Of C,(
R4988 T10001 T9999 arg2Of s,(
R4989 T10001 T10000 arg1Of s,15
R4990 T10002 T9999 arg3Of ),(
R4991 T10004 T10003 arg1Of and,","
R4992 T10004 T10015 arg1Of and,and
R4993 T10005 T10004 arg2Of at,and
R4994 T10007 T10005 arg2Of C,at
R4995 T10007 T10006 arg1Of C,72°
R4996 T10007 T10008 arg1Of C,(
R4997 T10007 T10012 arg1Of C,for
R4998 T10010 T10008 arg2Of s,(
R4999 T10010 T10009 arg1Of s,10
R5000 T10011 T10008 arg3Of ),(
R5001 T10013 T10012 arg2Of SOCS1,for
R5002 T10015 T10014 arg1Of and,;
R5003 T10016 T10032 arg1Of at,and
R5004 T10018 T10016 arg2Of C,at
R5005 T10018 T10017 arg1Of C,96°
R5006 T10018 T10019 arg1Of C,(
R5007 T10018 T10023 arg1Of C,","
R5008 T10018 T10024 arg1Of C,at
R5009 T10021 T10019 arg2Of s,(
R5010 T10021 T10020 arg1Of s,10
R5011 T10022 T10019 arg3Of ),(
R5012 T10026 T10024 arg2Of C,at
R5013 T10026 T10025 arg1Of C,68°
R5014 T10026 T10027 arg1Of C,(
R5015 T10029 T10027 arg2Of s,(
R5016 T10029 T10028 arg1Of s,15
R5017 T10030 T10027 arg3Of ),(
R5018 T10032 T10015 arg2Of and,and
R5019 T10032 T10031 arg1Of and,","
R5020 T10033 T10032 arg2Of at,and
R5021 T10035 T10033 arg2Of C,at
R5022 T10035 T10034 arg1Of C,72°
R5023 T10035 T10036 arg1Of C,(
R5024 T10035 T10040 arg1Of C,for
R5025 T10038 T10036 arg2Of s,(
R5026 T10038 T10037 arg1Of s,15
R5027 T10039 T10036 arg3Of ),(
R5028 T10041 T10040 arg2Of SOCS3,for
R5029 T10043 T10042 arg1Of curves,Amplification
R5030 T10043 T10044 arg1Of curves,of
R5031 T10043 T10051 arg1Of curves,were
R5032 T10043 T10052 arg2Of curves,obtained
R5033 T10047 T10046 arg1Of values,fluorescence
R5034 T10047 T10048 arg1Of values,versus
R5035 T10048 T10044 arg2Of versus,of
R5036 T10048 T10045 arg1Of versus,the
R5037 T10050 T10048 arg2Of number,versus
R5038 T10050 T10049 arg1Of number,cycle
R5039 T10052 T10051 arg2Of obtained,were
R5040 T10052 T10054 arg1Of obtained,and
R5041 T10054 T10053 arg1Of and,","
R5042 T10058 T10055 arg1Of analysis,a
R5043 T10058 T10056 arg1Of analysis,melting
R5044 T10058 T10057 arg1Of analysis,curve
R5045 T10058 T10059 arg1Of analysis,was
R5046 T10058 T10061 arg2Of analysis,performed
R5047 T10061 T10054 arg2Of performed,and
R5048 T10061 T10059 arg2Of performed,was
R5049 T10061 T10060 arg1Of performed,then
R5050 T10063 T10062 arg1Of levels,The
R5051 T10063 T10064 arg1Of levels,of
R5052 T10063 T10069 arg1Of levels,were
R5053 T10063 T10070 arg2Of levels,determined
R5054 T10065 T10066 arg1Of SOCS1,and
R5055 T10067 T10066 arg2Of SOCS3,and
R5056 T10068 T10064 arg2Of expression,of
R5057 T10068 T10065 arg1Of expression,SOCS1
R5058 T10068 T10067 arg1Of expression,SOCS3
R5059 T10070 T10069 arg2Of determined,were
R5060 T10070 T10071 arg1Of determined,by
R5061 T10072 T10071 arg2Of normalizing,by
R5062 T10073 T10072 arg2Of relative,normalizing
R5063 T10073 T10074 arg1Of relative,to
R5064 T10076 T10074 arg2Of expression,to
R5065 T10076 T10075 arg1Of expression,β-actin
R5066 T10078 T10079 arg1Of forward,and
R5067 T10080 T10079 arg2Of reverse,and
R5068 T10081 T10077 arg1Of primers,The
R5069 T10081 T10078 arg1Of primers,forward
R5070 T10081 T10080 arg1Of primers,reverse
R5071 T10081 T10082 arg1Of primers,were
R5072 T10081 T10084 arg1Of primers,follows
R5073 T10082 T10083 arg1Of were,as
R5074 T10083 T10086 arg1Of as,for
R5075 T10083 T10088 arg1Of as,","
R5076 T10083 T10094 arg1Of as,;
R5077 T10083 T10095 arg1Of as,for
R5078 T10083 T10105 arg1Of as,for
R5079 T10083 T10112 arg1Of as,'
R5080 T10084 T10083 arg2Of follows,as
R5081 T10084 T10085 arg1Of follows,:
R5082 T10087 T10086 arg2Of β-actin,for
R5083 T10089 T10090 arg1Of 5'-GTGGGGCGCCCCAGGCACCA-3,'
R5084 T10089 T10091 arg1Of 5'-GTGGGGCGCCCCAGGCACCA-3,and
R5085 T10091 T10082 arg2Of and,were
R5086 T10091 T10084 arg2Of and,follows
R5087 T10092 T10091 arg2Of 5'-CTCCTTAATGTCACGCACGATTTC-3,and
R5088 T10092 T10093 arg1Of 5'-CTCCTTAATGTCACGCACGATTTC-3,'
R5089 T10095 T10104 arg1Of for,and
R5090 T10096 T10097 arg1Of SOCS1,","
R5091 T10097 T10100 arg1Of ",",and
R5092 T10098 T10097 arg2Of 5'-AGACCCCTTCTCACCTCTTG-3,","
R5093 T10098 T10099 arg1Of 5'-AGACCCCTTCTCACCTCTTG-3,'
R5094 T10100 T10095 arg2Of and,for
R5095 T10100 T10102 arg1Of and,'
R5096 T10101 T10100 arg2Of 5'-GCACAGCAGAAAAATAAAGC-3,and
R5097 T10104 T10103 arg1Of and,;
R5098 T10105 T10104 arg2Of for,and
R5099 T10106 T10107 arg1Of SOCS3,","
R5100 T10107 T10110 arg1Of ",",and
R5101 T10108 T10107 arg2Of 5'-CCCGCCGGCACCTTTCTG-3,","
R5102 T10108 T10109 arg1Of 5'-CCCGCCGGCACCTTTCTG-3,'
R5103 T10110 T10105 arg2Of and,for
R5104 T10111 T10110 arg2Of 5'-AGGGGCCGGCTCAACACC-3,and
R5501 T11085 T11083 arg1Of analysis,Western
R5502 T11085 T11084 arg1Of analysis,blot
R5503 T11088 T11086 arg1Of cells,CD4+
R5504 T11088 T11087 arg1Of cells,T
R5505 T11088 T11089 arg1Of cells,were
R5506 T11088 T11090 arg2Of cells,stimulated
R5507 T11090 T11089 arg2Of stimulated,were
R5508 T11090 T11091 arg1Of stimulated,for
R5509 T11090 T11094 arg1Of stimulated,by
R5510 T11090 T11102 arg1Of stimulated,at
R5511 T11093 T11091 arg2Of min,for
R5512 T11093 T11092 arg1Of min,20
R5513 T11097 T11094 arg2Of concentrations,by
R5514 T11097 T11095 arg1Of concentrations,the
R5515 T11097 T11096 arg2Of concentrations,indicated
R5516 T11097 T11098 arg1Of concentrations,of
R5517 T11099 T11100 arg1Of IL-10,and
R5518 T11100 T11098 arg2Of and,of
R5519 T11101 T11100 arg2Of IL-6,and
R5520 T11104 T11102 arg2Of density,at
R5521 T11104 T11103 arg1Of density,a
R5522 T11104 T11105 arg1Of density,of
R5523 T11104 T11110 arg1Of density,in
R5524 T11108 T11107 arg1Of 105,×
R5525 T11109 T11105 arg2Of cells,of
R5526 T11109 T11106 arg1Of cells,5
R5527 T11109 T11108 arg1Of cells,105
R5528 T11111 T11112 arg1Of 0.5,ml
R5529 T11114 T11110 arg2Of medium,in
R5530 T11114 T11111 arg1Of medium,0.5
R5531 T11114 T11113 arg1Of medium,culture
R5532 T11114 T11115 arg1Of medium,with
R5533 T11116 T11117 arg1Of 10,%
R5534 T11118 T11115 arg2Of FCS,with
R5535 T11118 T11116 arg1Of FCS,10
R5536 T11120 T11119 arg1Of examine,To
R5537 T11122 T11120 arg2Of effect,examine
R5538 T11122 T11121 arg1Of effect,the
R5539 T11122 T11123 arg1Of effect,of
R5540 T11122 T11126 arg1Of effect,on
R5541 T11125 T11123 arg2Of IL-6,of
R5542 T11125 T11124 arg1Of IL-6,serum
R5543 T11128 T11126 arg2Of phosphorylation,on
R5544 T11128 T11127 arg1Of phosphorylation,STAT
R5545 T11133 T11130 arg1Of cells,normal
R5546 T11133 T11131 arg1Of cells,CD4+
R5547 T11133 T11132 arg1Of cells,T
R5548 T11133 T11134 arg1Of cells,were
R5549 T11133 T11135 arg2Of cells,stimulated
R5550 T11135 T11119 modOf stimulated,To
R5551 T11135 T11129 arg1Of stimulated,","
R5552 T11135 T11134 arg2Of stimulated,were
R5553 T11135 T11136 arg1Of stimulated,for
R5554 T11135 T11139 arg1Of stimulated,with
R5555 T11138 T11136 arg2Of min,for
R5556 T11138 T11137 arg1Of min,20
R5557 T11140 T11141 arg1Of 30,%
R5558 T11144 T11139 arg2Of serum,with
R5559 T11144 T11140 arg1Of serum,30
R5560 T11144 T11142 arg1Of serum,active
R5561 T11144 T11143 arg1Of serum,RA
R5562 T11144 T11145 arg1Of serum,in
R5563 T11144 T11148 arg1Of serum,with
R5564 T11147 T11145 arg2Of medium,in
R5565 T11147 T11146 arg1Of medium,culture
R5566 T11150 T11148 arg2Of μg/ml,with
R5567 T11150 T11149 arg1Of μg/ml,40
R5568 T11150 T11151 arg1Of μg/ml,neutralizing
R5569 T11155 T11152 arg1Of antibody,goat
R5570 T11155 T11153 arg1Of antibody,anti-IL-6
R5571 T11155 T11154 arg1Of antibody,polyclonal
R5572 T11155 T11156 arg1Of antibody,(
R5573 T11155 T11167 arg1Of antibody,or
R5574 T11157 T11156 arg2Of IgG,(
R5575 T11157 T11158 arg1Of IgG,;
R5576 T11157 T11164 arg1Of IgG,","
R5577 T11157 T11165 arg1Of IgG,USA
R5578 T11159 T11160 arg1Of Techne,","
R5579 T11160 T11162 arg1Of ",",","
R5580 T11161 T11160 arg2Of Princeton,","
R5581 T11162 T11158 arg2Of ",",;
R5582 T11163 T11162 arg2Of NJ,","
R5583 T11166 T11156 arg3Of ),(
R5584 T11167 T11151 arg2Of or,neutralizing
R5585 T11170 T11167 arg2Of IgG,or
R5586 T11170 T11168 arg1Of IgG,control
R5587 T11170 T11169 arg1Of IgG,goat
R5588 T11170 T11171 arg1Of IgG,(
R5589 T11172 T11171 arg2Of Techne,(
R5590 T11173 T11171 arg3Of ),(
R5591 T11176 T11174 arg1Of lysates,Whole
R5592 T11176 T11175 arg1Of lysates,cell
R5593 T11176 T11177 arg1Of lysates,were
R5594 T11176 T11178 arg2Of lysates,prepared
R5595 T11178 T11177 arg2Of prepared,were
R5596 T11178 T11179 arg1Of prepared,by
R5597 T11180 T11179 arg2Of placing,by
R5598 T11180 T11182 arg1Of placing,in
R5599 T11181 T11180 arg2Of cells,placing
R5600 T11185 T11182 arg2Of SDS,in
R5601 T11185 T11183 arg1Of SDS,100
R5602 T11185 T11184 arg1Of SDS,μl
R5603 T11185 T11186 arg1Of SDS,lysing
R5604 T11187 T11186 arg2Of buffer,lysing
R5605 T11187 T11188 arg1Of buffer,(
R5606 T11189 T11190 arg1Of 62.5,mM
R5607 T11191 T11189 arg1Of Tris–HCl,62.5
R5608 T11191 T11192 arg1Of Tris–HCl,[
R5609 T11191 T11196 arg1Of Tris–HCl,","
R5610 T11193 T11192 arg2Of pH,[
R5611 T11193 T11194 arg1Of pH,6.8
R5612 T11195 T11192 arg3Of ],[
R5613 T11196 T11188 arg2Of ",",(
R5614 T11197 T11198 arg1Of 2,%
R5615 T11199 T11197 arg1Of SDS,2
R5616 T11199 T11200 arg1Of SDS,","
R5617 T11200 T11204 arg1Of ",",","
R5618 T11201 T11202 arg1Of 10,%
R5619 T11203 T11200 arg2Of glycerol,","
R5620 T11203 T11201 arg1Of glycerol,10
R5621 T11204 T11208 arg1Of ",",","
R5622 T11205 T11206 arg1Of 50,mM
R5623 T11207 T11204 arg2Of dithiothreitol,","
R5624 T11207 T11205 arg1Of dithiothreitol,50
R5625 T11208 T11196 arg2Of ",",","
R5626 T11209 T11210 arg1Of 0.1,%
R5627 T11212 T11208 arg2Of blue,","
R5628 T11212 T11209 arg1Of blue,0.1
R5629 T11212 T11211 arg1Of blue,bromphenol
R5630 T11213 T11188 arg3Of ),(
R5631 T11215 T11214 arg1Of 20,Then
R5632 T11218 T11215 arg1Of samples,20
R5633 T11218 T11216 arg1Of samples,μl
R5634 T11218 T11217 arg1Of samples,protein
R5635 T11218 T11219 arg1Of samples,were
R5636 T11218 T11220 arg2Of samples,fractionated
R5637 T11218 T11227 arg1Of samples,were
R5638 T11218 T11228 arg2Of samples,transferred
R5639 T11220 T11219 arg2Of fractionated,were
R5640 T11220 T11221 arg1Of fractionated,on
R5641 T11220 T11226 arg1Of fractionated,and
R5642 T11222 T11223 arg1Of 10,%
R5643 T11225 T11221 arg2Of gels,on
R5644 T11225 T11222 arg1Of gels,10
R5645 T11225 T11224 arg1Of gels,SDS-polyacrylamide
R5646 T11226 T11232 arg1Of and,(
R5647 T11226 T11240 arg1Of and,and
R5648 T11228 T11226 arg2Of transferred,and
R5649 T11228 T11227 arg2Of transferred,were
R5650 T11228 T11229 modOf transferred,to
R5651 T11230 T11229 arg1Of nitrocellulose,to
R5652 T11231 T11230 arg2Of membranes,nitrocellulose
R5653 T11233 T11234 arg1Of Amersham,","
R5654 T11234 T11236 arg1Of ",",","
R5655 T11235 T11234 arg2Of Buckinghamshire,","
R5656 T11236 T11232 arg2Of ",",(
R5657 T11237 T11236 arg2Of UK,","
R5658 T11238 T11232 arg3Of ),(
R5659 T11240 T11239 arg1Of and,","
R5660 T11242 T11241 arg1Of membrane,the
R5661 T11242 T11243 arg1Of membrane,was
R5662 T11242 T11244 arg2Of membrane,blocked
R5663 T11244 T11240 arg2Of blocked,and
R5664 T11244 T11243 arg2Of blocked,was
R5665 T11244 T11245 arg1Of blocked,with
R5666 T11249 T11245 arg2Of milk,with
R5667 T11249 T11246 arg1Of milk,5
R5668 T11249 T11247 arg1Of milk,%
R5669 T11249 T11248 arg1Of milk,skim
R5670 T11249 T11250 arg1Of milk,in
R5671 T11252 T11250 arg2Of saline,in
R5672 T11252 T11251 arg1Of saline,Tris-buffered
R5673 T11252 T11253 arg1Of saline,with
R5674 T11255 T11254 arg1Of %,0.1
R5675 T11255 T11256 arg1Of %,Tween
R5676 T11257 T11253 arg2Of 20,with
R5677 T11257 T11255 arg1Of 20,%
R5678 T11259 T11258 arg1Of phosphorylation,Tyrosine
R5679 T11259 T11260 arg1Of phosphorylation,of
R5680 T11259 T11264 arg1Of phosphorylation,was
R5681 T11259 T11265 arg2Of phosphorylation,detected
R5682 T11259 T11266 arg1Of phosphorylation,using
R5683 T11261 T11262 arg1Of STAT1,and
R5684 T11262 T11260 arg2Of and,of
R5685 T11263 T11262 arg2Of STAT3,and
R5686 T11265 T11264 arg2Of detected,was
R5687 T11266 T11265 arg3Of using,detected
R5688 T11266 T11281 arg1Of using,according
R5689 T11269 T11266 arg2Of kits,using
R5690 T11269 T11267 arg1Of kits,commercial
R5691 T11269 T11268 arg1Of kits,available
R5692 T11269 T11270 arg1Of kits,(
R5693 T11273 T11270 arg2Of Technology,(
R5694 T11273 T11271 arg1Of Technology,Cell
R5695 T11273 T11272 arg1Of Technology,Signaling
R5696 T11273 T11274 arg1Of Technology,","
R5697 T11273 T11278 arg1Of Technology,","
R5698 T11273 T11279 arg1Of Technology,USA
R5699 T11275 T11276 arg1Of Beverly,","
R5700 T11276 T11274 arg2Of ",",","
R5701 T11277 T11276 arg2Of MA,","
R5702 T11280 T11270 arg3Of ),(
R5703 T11282 T11281 arg2Of to,according
R5704 T11284 T11283 arg1Of manufacturer,the
R5705 T11284 T11285 arg2Of manufacturer,'s
R5706 T11286 T11282 arg2Of instructions,to
R5707 T11286 T11285 arg1Of instructions,'s
R5708 T11290 T11289 arg1Of membrane,the
R5709 T11290 T11291 arg1Of membrane,was
R5710 T11290 T11292 arg2Of membrane,incubated
R5711 T11292 T11287 arg1Of incubated,Briefly
R5712 T11292 T11288 arg1Of incubated,","
R5713 T11292 T11291 arg2Of incubated,was
R5714 T11292 T11293 arg1Of incubated,with
R5715 T11295 T11296 arg1Of antibodies,(
R5716 T11298 T11296 arg2Of IgG,(
R5717 T11298 T11297 arg1Of IgG,rabbit
R5718 T11299 T11296 arg3Of ),(
R5719 T11301 T11293 arg2Of antibody,with
R5720 T11301 T11294 arg1Of antibody,the
R5721 T11301 T11295 arg1Of antibody,antibodies
R5722 T11301 T11300 arg1Of antibody,anti-STAT1
R5723 T11301 T11302 arg1Of antibody,","
R5724 T11304 T11302 arg2Of tyrosine,","
R5725 T11304 T11303 arg1Of tyrosine,anti-phosphorylated
R5726 T11304 T11305 arg1Of tyrosine,701
R5727 T11304 T11306 arg1Of tyrosine,of
R5728 T11304 T11320 arg1Of tyrosine,","
R5729 T11304 T11321 arg2Of tyrosine,diluted
R5730 T11308 T11307 arg1Of antibody,STAT1
R5731 T11308 T11309 arg1Of antibody,","
R5732 T11309 T11313 arg1Of ",",and
R5733 T11311 T11309 arg2Of antibody,","
R5734 T11311 T11310 arg1Of antibody,anti-STAT3
R5735 T11313 T11306 arg2Of and,of
R5736 T11313 T11312 arg1Of and,","
R5737 T11313 T11317 arg1Of and,of
R5738 T11315 T11313 arg2Of tyrosine,and
R5739 T11315 T11314 arg1Of tyrosine,anti-phosphorylated
R5740 T11315 T11316 arg1Of tyrosine,705
R5741 T11319 T11317 arg2Of antibody,of
R5742 T11319 T11318 arg1Of antibody,STAT3
R5743 T11321 T11322 arg1Of diluted,as
R5744 T11323 T11322 arg2Of recommended,as
R5745 T11323 T11324 arg1Of recommended,at
R5746 T11325 T11324 arg2Of 1/2000,at
R5747 T11325 T11326 arg1Of 1/2000,with
R5748 T11328 T11326 arg2Of saline,with
R5749 T11328 T11327 arg1Of saline,Tris-buffered
R5750 T11328 T11329 arg1Of saline,with
R5751 T11328 T11334 arg1Of saline,with
R5752 T11331 T11330 arg1Of %,0.1
R5753 T11333 T11329 arg2Of 20,with
R5754 T11333 T11331 arg1Of 20,%
R5755 T11333 T11332 arg1Of 20,Tween
R5756 T11335 T11336 arg1Of 5,%
R5757 T11337 T11334 arg2Of BSA,with
R5758 T11337 T11335 arg1Of BSA,5
R5759 T11339 T11338 arg1Of binding,Antibody
R5760 T11339 T11340 arg1Of binding,was
R5761 T11339 T11341 arg2Of binding,detected
R5762 T11339 T11365 arg1Of binding,was
R5763 T11339 T11366 arg2Of binding,revealed
R5764 T11341 T11340 arg2Of detected,was
R5765 T11341 T11364 arg1Of detected,and
R5766 T11347 T11341 arg1Of antibody,detected
R5767 T11347 T11342 arg2Of antibody,by
R5768 T11347 T11343 arg1Of antibody,horseradish
R5769 T11347 T11344 arg1Of antibody,peroxidase-conjugated
R5770 T11347 T11345 arg1Of antibody,anti-rabbit
R5771 T11347 T11346 arg1Of antibody,IgG
R5772 T11347 T11348 arg2Of antibody,diluted
R5773 T11348 T11349 arg1Of diluted,at
R5774 T11350 T11349 arg2Of 1/4000,at
R5775 T11350 T11351 arg1Of 1/4000,with
R5776 T11353 T11351 arg2Of saline,with
R5777 T11353 T11352 arg1Of saline,Tris-buffered
R5778 T11353 T11354 arg1Of saline,with
R5779 T11356 T11355 arg1Of %,0.1
R5780 T11358 T11359 arg1Of 20,with
R5781 T11361 T11359 arg2Of %,with
R5782 T11361 T11360 arg1Of %,5
R5783 T11362 T11354 arg2Of BSA,with
R5784 T11362 T11356 arg1Of BSA,%
R5785 T11362 T11357 arg1Of BSA,Tween
R5786 T11362 T11358 arg1Of BSA,20
R5787 T11364 T11363 arg1Of and,","
R5788 T11366 T11364 arg2Of revealed,and
R5789 T11366 T11365 arg2Of revealed,was
R5790 T11366 T11367 modOf revealed,using
R5791 T11370 T11367 arg2Of system,using
R5792 T11370 T11368 arg1Of system,the
R5793 T11370 T11369 arg1Of system,chemiluminescence
R5794 T11372 T11371 arg1Of bands,Protein
R5795 T11372 T11373 arg1Of bands,were
R5796 T11372 T11374 arg2Of bands,quantified
R5797 T11374 T11373 arg2Of quantified,were
R5798 T11374 T11377 modOf quantified,using
R5799 T11374 T11381 arg1Of quantified,and
R5800 T11376 T11374 arg1Of densitometry,quantified
R5801 T11376 T11375 arg2Of densitometry,by
R5802 T11379 T11377 arg2Of analysis,using
R5803 T11379 T11378 arg1Of analysis,NIH-Image
R5804 T11381 T11380 arg1Of and,","
R5805 T11383 T11382 arg1Of phosphorylation,STAT
R5806 T11383 T11384 arg1Of phosphorylation,was
R5807 T11383 T11385 arg2Of phosphorylation,compared
R5808 T11385 T11381 arg2Of compared,and
R5809 T11385 T11384 arg2Of compared,was
R5810 T11385 T11386 arg1Of compared,with
R5811 T11389 T11386 arg2Of amount,with
R5812 T11389 T11387 arg1Of amount,the
R5813 T11389 T11388 arg1Of amount,total
R5814 T11389 T11390 arg1Of amount,of
R5815 T11392 T11390 arg2Of protein,of
R5816 T11392 T11391 arg1Of protein,STAT
R5817 T11395 T11393 arg1Of cells,IFN-γ-stimulated
R5818 T11395 T11394 arg1Of cells,Hela
R5819 T11395 T11396 arg1Of cells,were
R5820 T11395 T11397 arg2Of cells,used
R5821 T11397 T11396 arg2Of used,were
R5822 T11397 T11398 arg1Of used,as
R5823 T11401 T11398 arg2Of control,as
R5824 T11401 T11399 arg1Of control,a
R5825 T11401 T11400 arg1Of control,positive
R5826 T11401 T11402 arg1Of control,for
R5827 T11404 T11402 arg2Of phosophorylation,for
R5828 T11404 T11403 arg1Of phosophorylation,STAT1
R6209 T11978 T11977 arg1Of analysis,Statistical
R6210 T11979 T11980 arg1Of Data,are
R6211 T11979 T11981 arg2Of Data,expressed
R6212 T11981 T11980 arg2Of expressed,are
R6213 T11981 T11982 arg1Of expressed,as
R6214 T11985 T11982 arg2Of value,as
R6215 T11985 T11983 arg1Of value,the
R6216 T11985 T11984 arg1Of value,mean
R6217 T11988 T11982 arg3Of error,as
R6218 T11988 T11986 arg1Of error,±
R6219 T11988 T11987 arg1Of error,standard
R6220 T11988 T11989 arg1Of error,of
R6221 T11991 T11992 arg1Of mean,or
R6222 T11993 T11992 arg2Of box,or
R6223 T11994 T11989 arg2Of plots,of
R6224 T11994 T11990 arg1Of plots,the
R6225 T11994 T11991 arg1Of plots,mean
R6226 T11994 T11993 arg1Of plots,box
R6227 T11997 T11995 arg1Of significance,The
R6228 T11997 T11996 arg1Of significance,statistical
R6229 T11997 T11998 arg1Of significance,of
R6230 T11997 T12000 arg1Of significance,between
R6231 T11997 T12003 arg1Of significance,was
R6232 T11997 T12004 arg2Of significance,determined
R6233 T11999 T11998 arg2Of differences,of
R6234 T12002 T12000 arg2Of groups,between
R6235 T12002 T12001 arg1Of groups,two
R6236 T12004 T12003 arg2Of determined,was
R6237 T12004 T12010 arg1Of determined,or
R6238 T12008 T12007 arg1Of U,Mann–Whitney
R6239 T12009 T12004 arg1Of test,determined
R6240 T12009 T12005 arg2Of test,by
R6241 T12009 T12006 arg1Of test,the
R6242 T12009 T12008 arg1Of test,U
R6243 T12012 T12011 arg1Of Wilcoxon,the
R6244 T12012 T12013 arg1Of Wilcoxon,signed
R6245 T12013 T12010 arg2Of signed,or
R6246 T12015 T12013 arg2Of test,signed
R6247 T12015 T12014 arg1Of test,rank
R6248 T12016 T12019 arg1Of P,was
R6249 T12016 T12020 arg2Of P,considered
R6250 T12016 T12021 arg1Of P,significant
R6251 T12017 T12018 arg1Of <,0.05
R6252 T12020 T12017 arg1Of considered,<
R6253 T12020 T12019 arg2Of considered,was
R6254 T12021 T12020 arg3Of significant,considered

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T9079 3981-3985 Protein denotes IL-2
T9078 3971-3976 Protein denotes IFN-γ
T8046 3294-3299 Protein denotes IFN-γ
T8045 3248-3253 Protein denotes IL-10
T8044 3201-3205 Protein denotes CD28
T8043 3096-3100 Protein denotes IL-6
T8042 2976-2979 Protein denotes CD4
T8041 2969-2974 Protein denotes IL-10
T8040 2936-2940 Protein denotes IL-6
T8039 2692-2697 Protein denotes IL-10
T8038 2608-2612 Protein denotes CD28
T8037 2297-2300 Protein denotes CD4
T8036 2281-2284 Protein denotes CD4
T7482 2198-2201 Protein denotes CD4
T7481 1575-1578 Protein denotes CD4
T7480 1461-1464 Protein denotes CD4
T7479 1391-1394 Protein denotes CD4
T7478 1205-1208 Protein denotes CD4
T8706 3795-3798 Protein denotes CD4
T8705 3494-3501 Protein denotes IL-10R1
T8704 3346-3353 Protein denotes IL-10R1
T9760 5806-5811 Protein denotes SOCS3
T9759 5731-5736 Protein denotes SOCS1
T9758 5654-5661 Protein denotes β-actin
T9757 5581-5588 Protein denotes β-actin
T9756 5521-5526 Protein denotes SOCS3
T9755 5511-5516 Protein denotes SOCS1
T9754 5358-5363 Protein denotes SOCS3
T9753 5289-5294 Protein denotes SOCS1
T9752 5222-5229 Protein denotes β-actin
T9751 4887-4890 Protein denotes Taq
T9750 4423-4426 Protein denotes CD4
T9084 4325-4329 Protein denotes IL-2
T9083 4315-4320 Protein denotes IFN-γ
T9082 4046-4049 Protein denotes CD4
T9081 4014-4018 Protein denotes IL-2
T9080 4004-4009 Protein denotes IFN-γ
T10978 7674-7679 Protein denotes STAT1
T10977 7610-7615 Protein denotes IFN-γ
T10976 7537-7541 Protein denotes STAT
T10975 7133-7138 Protein denotes STAT3
T10974 7077-7082 Protein denotes STAT3
T10973 7056-7061 Protein denotes STAT1
T10972 7004-7009 Protein denotes STAT1
T10971 6787-6792 Protein denotes STAT3
T10970 6777-6782 Protein denotes STAT1
T10969 6238-6242 Protein denotes IL-6
T10968 6118-6121 Protein denotes CD4
T10967 6081-6085 Protein denotes IL-6
T10966 5976-5980 Protein denotes IL-6
T10965 5966-5971 Protein denotes IL-10
T10964 5891-5894 Protein denotes CD4

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T11067 7610-7615 http://www.uniprot.org/uniprot/P01579 denotes IFN-γ
T11066 7133-7138 http://www.uniprot.org/uniprot/P40763 denotes STAT3
T11065 7077-7082 http://www.uniprot.org/uniprot/P40763 denotes STAT3
T11064 6787-6792 http://www.uniprot.org/uniprot/P40763 denotes STAT3
T11063 6238-6242 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T11062 6081-6085 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T11061 5976-5980 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T11060 5966-5971 http://www.uniprot.org/uniprot/P22301 denotes IL-10
T11059 6118-6121 http://www.uniprot.org/uniprot/P01730 denotes CD4
T11058 5891-5894 http://www.uniprot.org/uniprot/P01730 denotes CD4
T9797 5731-5736 http://www.uniprot.org/uniprot/O15524 denotes SOCS1
T9796 5511-5516 http://www.uniprot.org/uniprot/O15524 denotes SOCS1
T9795 5289-5294 http://www.uniprot.org/uniprot/O15524 denotes SOCS1
T9794 5654-5661 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T9793 5581-5588 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T9792 5222-5229 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T9791 4423-4426 http://www.uniprot.org/uniprot/P01730 denotes CD4
T8060 2524-2527 http://www.uniprot.org/uniprot/P20963 denotes CD3
T8059 2976-2979 http://www.uniprot.org/uniprot/P01730 denotes CD4
T8075 3294-3299 http://www.uniprot.org/uniprot/P01579 denotes IFN-γ
T8074 3096-3100 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T8073 2936-2940 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T8072 3248-3253 http://www.uniprot.org/uniprot/P22301 denotes IL-10
T8071 2969-2974 http://www.uniprot.org/uniprot/P22301 denotes IL-10
T8070 2692-2697 http://www.uniprot.org/uniprot/P22301 denotes IL-10
T8069 3201-3205 http://www.uniprot.org/uniprot/P10747 denotes CD28
T8068 2608-2612 http://www.uniprot.org/uniprot/P10747 denotes CD28
T8067 3184-3187 http://www.uniprot.org/uniprot/P09693 denotes CD3
T8066 2524-2527 http://www.uniprot.org/uniprot/P09693 denotes CD3
T8065 3184-3187 http://www.uniprot.org/uniprot/P07766 denotes CD3
T8064 2524-2527 http://www.uniprot.org/uniprot/P07766 denotes CD3
T8063 3184-3187 http://www.uniprot.org/uniprot/P04234 denotes CD3
T8062 2524-2527 http://www.uniprot.org/uniprot/P04234 denotes CD3
T8061 3184-3187 http://www.uniprot.org/uniprot/P20963 denotes CD3
T8058 2297-2300 http://www.uniprot.org/uniprot/P01730 denotes CD4
T8057 2281-2284 http://www.uniprot.org/uniprot/P01730 denotes CD4
T7493 2198-2201 http://www.uniprot.org/uniprot/P01730 denotes CD4
T7492 1575-1578 http://www.uniprot.org/uniprot/P01730 denotes CD4
T7491 1461-1464 http://www.uniprot.org/uniprot/P01730 denotes CD4
T7490 1391-1394 http://www.uniprot.org/uniprot/P01730 denotes CD4
T7489 1205-1208 http://www.uniprot.org/uniprot/P01730 denotes CD4
T8834 3795-3798 http://www.uniprot.org/uniprot/P01730 denotes CD4
T9177 4046-4049 http://www.uniprot.org/uniprot/P01730 denotes CD4
T9176 4325-4329 http://www.uniprot.org/uniprot/P60568 denotes IL-2
T9175 4014-4018 http://www.uniprot.org/uniprot/P60568 denotes IL-2
T9174 3981-3985 http://www.uniprot.org/uniprot/P60568 denotes IL-2
T9173 4315-4320 http://www.uniprot.org/uniprot/P01579 denotes IFN-γ
T9172 4004-4009 http://www.uniprot.org/uniprot/P01579 denotes IFN-γ
T9171 3971-3976 http://www.uniprot.org/uniprot/P01579 denotes IFN-γ

UBERON-AE

Id Subject Object Predicate Lexical cue
T10650 6700-6704 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk
T10649 6177-6182 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T10648 6075-6080 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T9355 4846-4857 http://purl.obolibrary.org/obo/UBERON_0001982 denotes capillaries
T7341 1766-1772 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T7340 1291-1296 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T7339 1229-1234 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T6557 1137-1141 http://purl.obolibrary.org/obo/UBERON_0001465 denotes knee
T6556 805-810 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T6555 563-568 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T10939 7542-7557 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T10938 6758-6773 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T10937 6094-6109 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T9735 4633-4646 http://purl.obolibrary.org/obo/GO_0034062 denotes transcriptase
T9734 4633-4646 http://purl.obolibrary.org/obo/GO_0003968 denotes transcriptase
T9733 4633-4646 http://purl.obolibrary.org/obo/GO_0003899 denotes transcriptase
T9732 4499-4501 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T9731 4388-4396 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T8699 3724-3726 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T8023 2727-2729 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T6866 1028-1031 http://purl.obolibrary.org/obo/GO_0007568 denotes age
T6865 157-160 http://purl.obolibrary.org/obo/GO_0007568 denotes age

GO-CC

Id Subject Object Predicate Lexical cue
T9765 4430-4435 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9088 4053-4058 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8711 3674-3682 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T8710 3674-3682 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T8709 3385-3390 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7488 2205-2210 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7487 2173-2178 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7486 1582-1587 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7485 1398-1403 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7484 1247-1252 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7483 1212-1217 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8056 2983-2988 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8055 2569-2574 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8054 2362-2367 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8053 2288-2293 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11010 7319-7331 http://purl.obolibrary.org/obo/GO_0071736 denotes IgG antibody
T11009 6943-6951 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T11008 6666-6674 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T11007 6615-6624 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T11006 7323-7331 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11005 7139-7147 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11004 7083-7091 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11003 7062-7070 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11002 7010-7018 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11001 6254-6262 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11000 7323-7331 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10999 7139-7147 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10998 7083-7091 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10997 7062-7070 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10996 7010-7018 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10995 6254-6262 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10994 7632-7637 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10993 6371-6376 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10992 6125-6130 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10991 6005-6010 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10990 5898-5903 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GO-MF

Id Subject Object Predicate Lexical cue
T9764 4633-4646 http://purl.obolibrary.org/obo/GO_0034062 denotes transcriptase
T9763 4633-4646 http://purl.obolibrary.org/obo/GO_0003968 denotes transcriptase
T9762 4633-4646 http://purl.obolibrary.org/obo/GO_0003899 denotes transcriptase
T9761 4499-4501 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T10989 7249-7256 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10988 7323-7331 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10987 7139-7147 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10986 7083-7091 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10985 7062-7070 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10984 7010-7018 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10983 6254-6262 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10982 6238-6242 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T10981 6081-6085 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T10980 5976-5980 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T10979 5966-5971 http://purl.obolibrary.org/obo/GO_0005141 denotes IL-10
T9087 4325-4329 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T9086 4014-4018 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T9085 3981-3985 http://purl.obolibrary.org/obo/GO_0005134 denotes IL-2
T8708 3724-3726 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T8707 3674-3682 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T8052 3096-3100 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T8051 2936-2940 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T8050 2727-2729 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T8049 3248-3253 http://purl.obolibrary.org/obo/GO_0005141 denotes IL-10
T8048 2969-2974 http://purl.obolibrary.org/obo/GO_0005141 denotes IL-10
T8047 2692-2697 http://purl.obolibrary.org/obo/GO_0005141 denotes IL-10

sentences

Id Subject Object Predicate Lexical cue
T10936 7610-7697 Sentence denotes IFN-γ-stimulated Hela cells were used as a positive control for STAT1 phosophorylation.
T10935 7461-7609 Sentence denotes Protein bands were quantified by densitometry using NIH-Image analysis, and STAT phosphorylation was compared with the total amount of STAT protein.
T10934 7240-7460 Sentence denotes Antibody binding was detected by horseradish peroxidase-conjugated anti-rabbit IgG antibody diluted at 1/4000 with Tris-buffered saline with 0.1% Tween 20 with 5% BSA, and was revealed using the chemiluminescence system.
T10933 6930-7239 Sentence denotes Briefly, the membrane was incubated with the antibodies (rabbit IgG) anti-STAT1 antibody, anti-phosphorylated tyrosine 701 of STAT1 antibody, anti-STAT3 antibody, and anti-phosphorylated tyrosine 705 of STAT3 antibody, diluted as recommended at 1/2000 with Tris-buffered saline with 0.1% Tween 20 with 5% BSA.
T10932 6749-6929 Sentence denotes Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology, Beverly, MA, USA) according to the manufacturer's instructions.
T10931 6500-6748 Sentence denotes Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20.
T10930 6327-6499 Sentence denotes Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue).
T10929 6050-6326 Sentence denotes To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne).
T10928 5891-6049 Sentence denotes CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS.
T10927 5869-5890 Sentence denotes Western blot analysis
T9730 5601-5867 Sentence denotes The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'.
T9729 5497-5600 Sentence denotes The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression.
T9728 5365-5496 Sentence denotes Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed.
T9727 5072-5364 Sentence denotes The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3.
T9726 4859-5071 Sentence denotes The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions.
T9725 4738-4858 Sentence denotes Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries.
T9724 4382-4737 Sentence denotes Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA).
T9723 4346-4381 Sentence denotes Isolation of mRNA and real-time PCR
T8022 3255-3315 Sentence denotes Culture supernatants were measured for IFN-γ concentrations.
T8021 3134-3254 Sentence denotes Cells were then stimulated for 36 hours with anti-CD3 mAb and anti-CD28 mAb in the presence or absence of 1 ng/ml IL-10.
T8020 2911-3133 Sentence denotes To examine the effect of IL-6 on T-cell responsiveness to IL-10, CD4+ T cells from healthy controls were incubated in culture medium with 10% FCS in the presence or absence of 10 ng/ml IL-6 (Becton Dickinson) for 36 hours.
T8019 2795-2910 Sentence denotes Culture supernatants were collected 36 hours later and cell-free samples were stored at -30°C until cytokine assay.
T8018 2565-2794 Sentence denotes The cells were incubated with 1 μg/ml anti-CD28 mAb (Immunotech) in the presence or absence of the indicated concentrations of IL-10 (Becton Dickinson, San Jose, CA, USA) at 37°C in a humidified atmosphere containing 5% CO2 [28].
T8017 2294-2564 Sentence denotes PB CD4+ T-cell populations were resuspended at a density of 1 × 106 cells/ml in culture medium with 10% FCS, and 0.5 ml cell suspensions were dispensed into the wells of 24-well microtiter plates (Coster) coated with 1 μg/ml anti-CD3 mAb (Immunotech, Marseille, France).
T8016 2270-2293 Sentence denotes Culture of CD4+ T cells
T11976 7939-7975 Sentence denotes P < 0.05 was considered significant.
T11975 7800-7938 Sentence denotes The statistical significance of differences between two groups was determined by the Mann–Whitney U test or the Wilcoxon signed rank test.
T11974 7720-7799 Sentence denotes Data are expressed as the mean value ± standard error of the mean or box plots.
T11973 7699-7719 Sentence denotes Statistical analysis
T9070 4290-4344 Sentence denotes The detection limits for IFN-γ and IL-2 were 15 pg/ml.
T9069 3986-4289 Sentence denotes Concentrations of IFN-γ and IL-2 in culture supernatants of CD4+ T cells were measured in duplicate by the quantitative sandwich ELISA using cytokine-specific capture with biotinylated detection mAb and recombinant cytokine proteins (all from Becton Dickinson), according to the manufacturer's protocol.
T9068 3955-3985 Sentence denotes Immunoassay for IFN-γ and IL-2
T8698 3883-3953 Sentence denotes Analysis was performed on a FACScan flow cytometer (Becton Dickinson).
T8697 3823-3882 Sentence denotes Cells were washed well with 1% FCS/PBS between incubations.
T8696 3734-3822 Sentence denotes Cells were then incubated with phycoerythrin-conjugated anti-CD4 mAb (Becton Dickinson).
T8695 3435-3733 Sentence denotes PBMC were incubated with saturating concentrations of anti-IL-10R1 mAb (IgG1; R&D systems, Minneapolis, MN, USA) or with isotype-matched control mAb (Immunotech), followed by incubation with FITC-conjugated goat anti-mouse IgG1 polyclonal antibody (Santa Cruz Biotechnologies, Santa Cruz, CA, USA).
T8694 3365-3434 Sentence denotes A sample of 5 × 105 cells of PBMC was resuspended in PBS with 1% FCS.
T8693 3317-3364 Sentence denotes Flow cytometric analysis for IL-10R1 expression
T7472 2160-2268 Sentence denotes Non-adherent cells were harvested and CD4+ T cells were purified by positive selection as already described.
T7471 1649-2159 Sentence denotes Briefly, fresh ST samples were fragmented and digested with collagenase and DNase for 1 hour at 37°C. After removing tissue debris, ST cell suspensions in culture medium (RPMI 1640 medium; Life Technologies, Gaithersburg, MD, USA) supplemented with 25 mM HEPES (2 mM L-glutamine, 2% nonessential amino acids, 100 IU/ml penicillin, and 100 mg/ml streptomycin; Life Technologies) with 10% heat-inactivated FCS (Life Technologies) were incubated at 37°C in six-well plates (Coster, Cambridge, MA, USA) for 45 min.
T7470 1575-1648 Sentence denotes CD4+ T cells were isolated from ST samples, as previously described [27].
T7469 1391-1574 Sentence denotes CD4+ T cells were purified from PBMC by positive selection using anti-CD4 mAb-coated magnetic beads (Miltenyi Biotec, Gladbach, Germany), according to the manufacturer's instructions.
T7468 1218-1390 Sentence denotes Peripheral blood mononuclear cells (PBMC) were prepared from heparinized blood samples by centrifugation over Ficoll-Hypaque density gradients (Pharmacia, Uppsala, Sweden).
T7467 1192-1217 Sentence denotes Isolation of CD4+ T cells
T6864 1155-1190 Sentence denotes All patients gave informed consent.
T6863 1072-1154 Sentence denotes ST samples were obtained from three RA patients undergoing total knee replacement.
T6862 977-1071 Sentence denotes Sixteen healthy volunteers (11 women and five men; age, 45.8 ± 11.2 years) served as controls.
T6861 713-976 Sentence denotes 24 patients with active disease, who had multiple tender and/or swollen joints and elevated serum CRP level (≥ 10 mg/l); and eight patients with inactive disease, who satisfied the American College of Rheumatology preliminary criteria for clinical remission [26].
T6860 674-712 Sentence denotes Patients were divided into two groups:
T6859 423-673 Sentence denotes Clinical parameters in the study patients were as follows (mean ± standard deviation): erythrocyte sedimentation rate, 55.9 ± 35.4 mm/hour; serum C-reactive protein (CRP) level, 32.0 ± 32.0 mg/l; and IgM class rheumatoid factor titer, 142 ± 158 U/ml.
T6858 324-422 Sentence denotes All patients were receiving prednisolone (≤ 7.5 mg/day) and disease-modifying antirheumatic drugs.
T6857 44-323 Sentence denotes The total patient population consisted of 32 patients with RA (25 women and seven men; mean ± standard deviation age, 52.8 ± 12.4 years) diagnosed according to the revised 1987 criteria of the American College of Rheumatology (formally, the American Rheumatism Association) [25].
T6856 23-43 Sentence denotes Patients and samples
T36 0-21 Sentence denotes Materials and methods
T37 23-43 Sentence denotes Patients and samples
T38 44-323 Sentence denotes The total patient population consisted of 32 patients with RA (25 women and seven men; mean ± standard deviation age, 52.8 ± 12.4 years) diagnosed according to the revised 1987 criteria of the American College of Rheumatology (formally, the American Rheumatism Association) [25].
T39 324-422 Sentence denotes All patients were receiving prednisolone (≤ 7.5 mg/day) and disease-modifying antirheumatic drugs.
T40 423-673 Sentence denotes Clinical parameters in the study patients were as follows (mean ± standard deviation): erythrocyte sedimentation rate, 55.9 ± 35.4 mm/hour; serum C-reactive protein (CRP) level, 32.0 ± 32.0 mg/l; and IgM class rheumatoid factor titer, 142 ± 158 U/ml.
T41 674-712 Sentence denotes Patients were divided into two groups:
T42 713-976 Sentence denotes 24 patients with active disease, who had multiple tender and/or swollen joints and elevated serum CRP level (≥ 10 mg/l); and eight patients with inactive disease, who satisfied the American College of Rheumatology preliminary criteria for clinical remission [26].
T43 977-1071 Sentence denotes Sixteen healthy volunteers (11 women and five men; age, 45.8 ± 11.2 years) served as controls.
T44 1072-1154 Sentence denotes ST samples were obtained from three RA patients undergoing total knee replacement.
T45 1155-1190 Sentence denotes All patients gave informed consent.
T46 1192-1217 Sentence denotes Isolation of CD4+ T cells
T47 1218-1390 Sentence denotes Peripheral blood mononuclear cells (PBMC) were prepared from heparinized blood samples by centrifugation over Ficoll-Hypaque density gradients (Pharmacia, Uppsala, Sweden).
T48 1391-1574 Sentence denotes CD4+ T cells were purified from PBMC by positive selection using anti-CD4 mAb-coated magnetic beads (Miltenyi Biotec, Gladbach, Germany), according to the manufacturer's instructions.
T49 1575-1648 Sentence denotes CD4+ T cells were isolated from ST samples, as previously described [27].
T50 1649-1750 Sentence denotes Briefly, fresh ST samples were fragmented and digested with collagenase and DNase for 1 hour at 37°C.
T51 1751-2159 Sentence denotes After removing tissue debris, ST cell suspensions in culture medium (RPMI 1640 medium; Life Technologies, Gaithersburg, MD, USA) supplemented with 25 mM HEPES (2 mM L-glutamine, 2% nonessential amino acids, 100 IU/ml penicillin, and 100 mg/ml streptomycin; Life Technologies) with 10% heat-inactivated FCS (Life Technologies) were incubated at 37°C in six-well plates (Coster, Cambridge, MA, USA) for 45 min.
T52 2160-2268 Sentence denotes Non-adherent cells were harvested and CD4+ T cells were purified by positive selection as already described.
T53 2270-2293 Sentence denotes Culture of CD4+ T cells
T54 2294-2564 Sentence denotes PB CD4+ T-cell populations were resuspended at a density of 1 × 106 cells/ml in culture medium with 10% FCS, and 0.5 ml cell suspensions were dispensed into the wells of 24-well microtiter plates (Coster) coated with 1 μg/ml anti-CD3 mAb (Immunotech, Marseille, France).
T55 2565-2794 Sentence denotes The cells were incubated with 1 μg/ml anti-CD28 mAb (Immunotech) in the presence or absence of the indicated concentrations of IL-10 (Becton Dickinson, San Jose, CA, USA) at 37°C in a humidified atmosphere containing 5% CO2 [28].
T56 2795-2910 Sentence denotes Culture supernatants were collected 36 hours later and cell-free samples were stored at -30°C until cytokine assay.
T57 2911-3133 Sentence denotes To examine the effect of IL-6 on T-cell responsiveness to IL-10, CD4+ T cells from healthy controls were incubated in culture medium with 10% FCS in the presence or absence of 10 ng/ml IL-6 (Becton Dickinson) for 36 hours.
T58 3134-3254 Sentence denotes Cells were then stimulated for 36 hours with anti-CD3 mAb and anti-CD28 mAb in the presence or absence of 1 ng/ml IL-10.
T59 3255-3315 Sentence denotes Culture supernatants were measured for IFN-γ concentrations.
T60 3317-3364 Sentence denotes Flow cytometric analysis for IL-10R1 expression
T61 3365-3434 Sentence denotes A sample of 5 × 105 cells of PBMC was resuspended in PBS with 1% FCS.
T62 3435-3733 Sentence denotes PBMC were incubated with saturating concentrations of anti-IL-10R1 mAb (IgG1; R&D systems, Minneapolis, MN, USA) or with isotype-matched control mAb (Immunotech), followed by incubation with FITC-conjugated goat anti-mouse IgG1 polyclonal antibody (Santa Cruz Biotechnologies, Santa Cruz, CA, USA).
T63 3734-3822 Sentence denotes Cells were then incubated with phycoerythrin-conjugated anti-CD4 mAb (Becton Dickinson).
T64 3823-3882 Sentence denotes Cells were washed well with 1% FCS/PBS between incubations.
T65 3883-3953 Sentence denotes Analysis was performed on a FACScan flow cytometer (Becton Dickinson).
T66 3955-3985 Sentence denotes Immunoassay for IFN-γ and IL-2
T67 3986-4289 Sentence denotes Concentrations of IFN-γ and IL-2 in culture supernatants of CD4+ T cells were measured in duplicate by the quantitative sandwich ELISA using cytokine-specific capture with biotinylated detection mAb and recombinant cytokine proteins (all from Becton Dickinson), according to the manufacturer's protocol.
T68 4290-4344 Sentence denotes The detection limits for IFN-γ and IL-2 were 15 pg/ml.
T69 4346-4381 Sentence denotes Isolation of mRNA and real-time PCR
T70 4382-4737 Sentence denotes Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA).
T71 4738-4858 Sentence denotes Real-time PCR was performed with the LightCycler Instrument (Roche Diagnostics, Penzberg, Germany) in glass capillaries.
T72 4859-5071 Sentence denotes The reaction mix containing Taq DNA polymerase and DNA double-strand-specific SYBR Green I dye (Lightcycler FastStart DNA Master SYBR Green I; Roche Diagnostics) and specific primers were added to cDNA dilutions.
T73 5072-5364 Sentence denotes The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3.
T74 5365-5496 Sentence denotes Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed.
T75 5497-5600 Sentence denotes The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression.
T76 5601-5867 Sentence denotes The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'.
T77 5869-5890 Sentence denotes Western blot analysis
T78 5891-6049 Sentence denotes CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS.
T79 6050-6326 Sentence denotes To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne).
T80 6327-6499 Sentence denotes Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue).
T81 6500-6748 Sentence denotes Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20.
T82 6749-6929 Sentence denotes Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology, Beverly, MA, USA) according to the manufacturer's instructions.
T83 6930-7239 Sentence denotes Briefly, the membrane was incubated with the antibodies (rabbit IgG) anti-STAT1 antibody, anti-phosphorylated tyrosine 701 of STAT1 antibody, anti-STAT3 antibody, and anti-phosphorylated tyrosine 705 of STAT3 antibody, diluted as recommended at 1/2000 with Tris-buffered saline with 0.1% Tween 20 with 5% BSA.
T84 7240-7460 Sentence denotes Antibody binding was detected by horseradish peroxidase-conjugated anti-rabbit IgG antibody diluted at 1/4000 with Tris-buffered saline with 0.1% Tween 20 with 5% BSA, and was revealed using the chemiluminescence system.
T85 7461-7609 Sentence denotes Protein bands were quantified by densitometry using NIH-Image analysis, and STAT phosphorylation was compared with the total amount of STAT protein.
T86 7610-7697 Sentence denotes IFN-γ-stimulated Hela cells were used as a positive control for STAT1 phosophorylation.
T87 7699-7719 Sentence denotes Statistical analysis
T88 7720-7799 Sentence denotes Data are expressed as the mean value ± standard error of the mean or box plots.
T89 7800-7938 Sentence denotes The statistical significance of differences between two groups was determined by the Mann–Whitney U test or the Wilcoxon signed rank test.
T90 7939-7975 Sentence denotes P < 0.05 was considered significant.

ICD10

Id Subject Object Predicate Lexical cue
T6867 294-304 http://purl.bioontology.org/ontology/ICD10/M79.0 denotes Rheumatism

simple1

Id Subject Object Predicate Lexical cue
T11082 7674-7679 Protein denotes STAT1
T11081 7610-7615 Protein denotes IFN-γ
T11080 7537-7541 Protein denotes STAT
T11079 7133-7138 Protein denotes STAT3
T11078 7077-7082 Protein denotes STAT3
T11077 7056-7061 Protein denotes STAT1
T11076 7004-7009 Protein denotes STAT1
T11075 6787-6792 Protein denotes STAT3
T11074 6777-6782 Protein denotes STAT1
T11073 6238-6242 Protein denotes IL-6
T11072 6118-6121 Protein denotes CD4
T11071 6081-6085 Protein denotes IL-6
T11070 5976-5980 Protein denotes IL-6
T11069 5966-5971 Protein denotes IL-10
T11068 5891-5894 Protein denotes CD4
T9808 5806-5811 Protein denotes SOCS3
T9807 5731-5736 Protein denotes SOCS1
T9806 5654-5661 Protein denotes β-actin
T9805 5581-5588 Protein denotes β-actin
T9804 5521-5526 Protein denotes SOCS3
T9803 5511-5516 Protein denotes SOCS1
T9802 5358-5363 Protein denotes SOCS3
T9801 5289-5294 Protein denotes SOCS1
T9800 5222-5229 Protein denotes β-actin
T9799 4887-4890 Protein denotes Taq
T9798 4423-4426 Protein denotes CD4
T9110 4325-4329 Protein denotes IL-2
T9109 4315-4320 Protein denotes IFN-γ
T9108 4046-4049 Protein denotes CD4
T9107 4014-4018 Protein denotes IL-2
T9106 4004-4009 Protein denotes IFN-γ
T9105 3981-3985 Protein denotes IL-2
T9104 3971-3976 Protein denotes IFN-γ
T8722 3795-3798 Protein denotes CD4
T8721 3494-3501 Protein denotes IL-10R1
T8720 3346-3353 Protein denotes IL-10R1
T8107 3294-3299 Protein denotes IFN-γ
T8106 3248-3253 Protein denotes IL-10
T8105 3201-3205 Protein denotes CD28
T8104 3096-3100 Protein denotes IL-6
T8103 2976-2979 Protein denotes CD4
T8102 2969-2974 Protein denotes IL-10
T8101 2936-2940 Protein denotes IL-6
T8100 2692-2697 Protein denotes IL-10
T8099 2608-2612 Protein denotes CD28
T8098 2297-2300 Protein denotes CD4
T8097 2281-2284 Protein denotes CD4
T7510 2198-2201 Protein denotes CD4
T7509 1575-1578 Protein denotes CD4
T7508 1461-1464 Protein denotes CD4
T7507 1391-1394 Protein denotes CD4
T7506 1205-1208 Protein denotes CD4

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T10543 5581-5588 Protein denotes β-actin
T10542 5521-5526 Protein denotes SOCS3
T10541 5511-5516 Protein denotes SOCS1
T10540 5358-5363 Protein denotes SOCS3
T10539 5289-5294 Protein denotes SOCS1
T10538 5222-5229 Protein denotes β-actin
T10537 4887-4890 Protein denotes Taq
T10536 4423-4426 Protein denotes CD4
T9287 4325-4329 Protein denotes IL-2
T9286 4315-4320 Protein denotes IFN-γ
T9285 4046-4049 Protein denotes CD4
T9284 4014-4018 Protein denotes IL-2
T9283 4004-4009 Protein denotes IFN-γ
T9282 3981-3985 Protein denotes IL-2
T9281 3971-3976 Protein denotes IFN-γ
T8982 3354-3364 Gene_expression denotes expression
T8981 3795-3798 Protein denotes CD4
T8980 3494-3501 Protein denotes IL-10R1
T8979 3346-3353 Protein denotes IL-10R1
T8585 3294-3299 Protein denotes IFN-γ
T8584 3201-3205 Protein denotes CD28
T8583 3248-3253 Protein denotes IL-10
T8582 3096-3100 Protein denotes IL-6
T8581 2976-2979 Protein denotes CD4
T8580 2969-2974 Protein denotes IL-10
T8579 2936-2940 Protein denotes IL-6
T8578 2692-2697 Protein denotes IL-10
T8577 2608-2612 Protein denotes CD28
T8576 2297-2300 Protein denotes CD4
T8575 2281-2284 Protein denotes CD4
T7953 2198-2201 Protein denotes CD4
T7952 1575-1578 Protein denotes CD4
T7951 1461-1464 Protein denotes CD4
T7950 1391-1394 Protein denotes CD4
T7949 1205-1208 Protein denotes CD4
T11911 7680-7696 Phosphorylation denotes phosophorylation
T11910 7662-7669 Regulation denotes control
T11909 7542-7557 Phosphorylation denotes phosphorylation
T11908 6758-6773 Phosphorylation denotes phosphorylation
T11907 7674-7679 Protein denotes STAT1
T11906 7610-7615 Protein denotes IFN-γ
T11905 7537-7541 Protein denotes STAT
T11904 7004-7009 Protein denotes STAT1
T11903 7133-7138 Protein denotes STAT3
T11902 7077-7082 Protein denotes STAT3
T11901 7056-7061 Protein denotes STAT1
T11900 6787-6792 Protein denotes STAT3
T11899 6777-6782 Protein denotes STAT1
T11898 6238-6242 Protein denotes IL-6
T11897 6118-6121 Protein denotes CD4
T11896 6081-6085 Protein denotes IL-6
T11895 5976-5980 Protein denotes IL-6
T11894 5966-5971 Protein denotes IL-10
T11893 5891-5894 Protein denotes CD4
T10549 5589-5599 Gene_expression denotes expression
T10548 5557-5568 Negative_regulation denotes normalizing
T10547 5527-5537 Gene_expression denotes expression
T10546 5654-5661 Protein denotes β-actin
T10545 5806-5811 Protein denotes SOCS3
T10544 5731-5736 Protein denotes SOCS1
R4656 T8979 T8982 themeOf IL-10R1,expression
R5461 T10541 T10547 themeOf SOCS1,expression
R5462 T10542 T10547 themeOf SOCS3,expression
R5463 T10543 T10549 themeOf β-actin,expression
R5464 T10549 T10548 themeOf expression,normalizing
R6204 T11899 T11908 themeOf STAT1,phosphorylation
R6205 T11900 T11908 themeOf STAT3,phosphorylation
R6206 T11905 T11909 themeOf STAT,phosphorylation
R6207 T11907 T11910 themeOf STAT1,control
R6208 T11907 T11911 themeOf STAT1,phosophorylation

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T11419 7674-7679 Protein denotes STAT1
T11422 7680-7696 Phosphorylation denotes phosophorylation
T11421 7542-7557 Phosphorylation denotes phosphorylation
T11420 6758-6773 Phosphorylation denotes phosphorylation
T11418 7610-7615 Protein denotes IFN-γ
T11417 7537-7541 Protein denotes STAT
T11416 7004-7009 Protein denotes STAT1
T11415 7133-7138 Protein denotes STAT3
T11414 7077-7082 Protein denotes STAT3
T11413 7056-7061 Protein denotes STAT1
T11412 6787-6792 Protein denotes STAT3
T11411 6777-6782 Protein denotes STAT1
T11410 6238-6242 Protein denotes IL-6
T11409 6118-6121 Protein denotes CD4
T11408 6081-6085 Protein denotes IL-6
T11407 5976-5980 Protein denotes IL-6
T11406 5966-5971 Protein denotes IL-10
T11405 5891-5894 Protein denotes CD4
T10125 5589-5599 Gene_expression denotes expression
T10124 5527-5537 Gene_expression denotes expression
T10123 5654-5661 Protein denotes β-actin
T10122 5806-5811 Protein denotes SOCS3
T10121 5731-5736 Protein denotes SOCS1
T10120 5581-5588 Protein denotes β-actin
T10119 5521-5526 Protein denotes SOCS3
T10118 5511-5516 Protein denotes SOCS1
T10117 5358-5363 Protein denotes SOCS3
T10116 5289-5294 Protein denotes SOCS1
T10115 5222-5229 Protein denotes β-actin
T10114 4887-4890 Protein denotes Taq
T10113 4423-4426 Protein denotes CD4
T8838 3354-3364 Gene_expression denotes expression
T8837 3795-3798 Protein denotes CD4
T8836 3494-3501 Protein denotes IL-10R1
T8835 3346-3353 Protein denotes IL-10R1
T9184 4325-4329 Protein denotes IL-2
T9183 4315-4320 Protein denotes IFN-γ
T9182 4046-4049 Protein denotes CD4
T9181 4014-4018 Protein denotes IL-2
T9180 4004-4009 Protein denotes IFN-γ
T9179 3981-3985 Protein denotes IL-2
T9178 3971-3976 Protein denotes IFN-γ
T8312 2926-2932 Regulation denotes effect
T8311 3294-3299 Protein denotes IFN-γ
T8310 3201-3205 Protein denotes CD28
T8309 3248-3253 Protein denotes IL-10
T8308 3096-3100 Protein denotes IL-6
T8307 2976-2979 Protein denotes CD4
T8306 2969-2974 Protein denotes IL-10
T8305 2936-2940 Protein denotes IL-6
T8304 2692-2697 Protein denotes IL-10
T8303 2608-2612 Protein denotes CD28
T8302 2297-2300 Protein denotes CD4
T8301 2281-2284 Protein denotes CD4
T7706 2198-2201 Protein denotes CD4
T7705 1575-1578 Protein denotes CD4
T7704 1461-1464 Protein denotes CD4
T7703 1391-1394 Protein denotes CD4
T7702 1205-1208 Protein denotes CD4
R4211 T8305 T8312 themeOf IL-6,effect
R4537 T8835 T8838 themeOf IL-10R1,expression
R5105 T10118 T10124 themeOf SOCS1,expression
R5106 T10119 T10124 themeOf SOCS3,expression
R5107 T10120 T10125 themeOf β-actin,expression
R5829 T11411 T11420 themeOf STAT1,phosphorylation
R5830 T11412 T11420 themeOf STAT3,phosphorylation
R5831 T11417 T11421 themeOf STAT,phosphorylation
R5832 T11419 T11422 themeOf STAT1,phosophorylation

DLUT931

Id Subject Object Predicate Lexical cue
T11777 7680-7696 Phosphorylation denotes phosophorylation
T11776 7542-7557 Phosphorylation denotes phosphorylation
T11775 7020-7039 Phosphorylation denotes anti-phosphorylated
T11774 6758-6773 Phosphorylation denotes phosphorylation
T11773 7674-7679 Protein denotes STAT1
T11772 7610-7615 Protein denotes IFN-γ
T11771 7537-7541 Protein denotes STAT
T11770 7004-7009 Protein denotes STAT1
T11769 7133-7138 Protein denotes STAT3
T11768 7077-7082 Protein denotes STAT3
T11767 7056-7061 Protein denotes STAT1
T11766 6787-6792 Protein denotes STAT3
T11765 6777-6782 Protein denotes STAT1
T11764 6238-6242 Protein denotes IL-6
T11763 6118-6121 Protein denotes CD4
T11762 6081-6085 Protein denotes IL-6
T11761 5976-5980 Protein denotes IL-6
T11760 5966-5971 Protein denotes IL-10
T11759 5891-5894 Protein denotes CD4
T10479 5654-5661 Protein denotes β-actin
T10478 5806-5811 Protein denotes SOCS3
T10477 5731-5736 Protein denotes SOCS1
T10476 5581-5588 Protein denotes β-actin
T10475 5521-5526 Protein denotes SOCS3
T10474 5511-5516 Protein denotes SOCS1
T10473 5358-5363 Protein denotes SOCS3
T10472 5289-5294 Protein denotes SOCS1
T10471 5222-5229 Protein denotes β-actin
T10470 4887-4890 Protein denotes Taq
T10469 4423-4426 Protein denotes CD4
T10481 5589-5599 Gene_expression denotes expression
T10480 5527-5537 Gene_expression denotes expression
T9254 4325-4329 Protein denotes IL-2
T9253 4315-4320 Protein denotes IFN-γ
T9252 4046-4049 Protein denotes CD4
T9251 4014-4018 Protein denotes IL-2
T9250 4004-4009 Protein denotes IFN-γ
T9249 3981-3985 Protein denotes IL-2
T9248 3971-3976 Protein denotes IFN-γ
T8842 3354-3364 Gene_expression denotes expression
T8841 3795-3798 Protein denotes CD4
T8840 3494-3501 Protein denotes IL-10R1
T8839 3346-3353 Protein denotes IL-10R1
T8533 3294-3299 Protein denotes IFN-γ
T8532 3201-3205 Protein denotes CD28
T8531 3248-3253 Protein denotes IL-10
T8530 3096-3100 Protein denotes IL-6
T8529 2976-2979 Protein denotes CD4
T8528 2969-2974 Protein denotes IL-10
T8527 2936-2940 Protein denotes IL-6
T8526 2692-2697 Protein denotes IL-10
T8525 2608-2612 Protein denotes CD28
T8524 2297-2300 Protein denotes CD4
T8523 2281-2284 Protein denotes CD4
T7916 2198-2201 Protein denotes CD4
T7915 1575-1578 Protein denotes CD4
T7914 1461-1464 Protein denotes CD4
T7913 1391-1394 Protein denotes CD4
T7912 1205-1208 Protein denotes CD4
R4538 T8839 T8842 themeOf IL-10R1,expression
R5451 T10474 T10480 themeOf SOCS1,expression
R5452 T10475 T10480 themeOf SOCS3,expression
R5453 T10476 T10481 themeOf β-actin,expression
R6169 T11765 T11774 themeOf STAT1,phosphorylation
R6170 T11766 T11774 themeOf STAT3,phosphorylation
R6171 T11767 T11775 themeOf STAT1,anti-phosphorylated
R6172 T11771 T11776 themeOf STAT,phosphorylation
R6173 T11773 T11777 themeOf STAT1,phosophorylation

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T11860 7187-7238 Entity denotes Tris-buffered saline with 0.1% Tween 20 with 5% BSA
T11859 7610-7615 Protein denotes IFN-γ
T11858 7097-7148 Entity denotes anti-phosphorylated tyrosine 705 of STAT3 antibody,
T11857 7072-7091 Protein denotes anti-STAT3 antibody
T11856 7020-7148 Entity denotes anti-phosphorylated tyrosine 701 of STAT1 antibody, anti-STAT3 antibody, and anti-phosphorylated tyrosine 705 of STAT3 antibody,
T11855 5966-5971 Protein denotes IL-10
T11854 7187-7200 Entity denotes Tris-buffered
T11853 6380-6390 Protein denotes 100 μl SDS
T11852 6971-6998 Entity denotes the antibodies (rabbit IgG)
T11851 7213-7238 Entity denotes 0.1% Tween 20 with 5% BSA
T11850 7133-7148 Protein denotes STAT3 antibody,
T11849 6999-7019 Entity denotes anti-STAT1 antibody,
T11848 6552-6555 Protein denotes SDS
T11847 6436-6454 Protein denotes SDS, 10% glycerol,
T11846 6548-6575 Entity denotes 10% SDS-polyacrylamide gels
T11845 7537-7541 Protein denotes STAT
T11844 6414-6422 Entity denotes Tris–HCl
T11843 6708-6728 Entity denotes Tris-buffered saline
T11842 7400-7407 Protein denotes 5% BSA,
T11841 6556-6570 Entity denotes polyacrylamide
T11840 7616-7637 Entity denotes stimulated Hela cells
T11839 6433-6498 Entity denotes 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue)
T11838 6089-6093 Protein denotes STAT
T11837 6243-6262 Protein denotes polyclonal antibody
T11836 5994-6010 Entity denotes of 5 × 105 cells
T11835 7355-7385 Entity denotes Tris-buffered saline with 0.1%
T11834 6174-6176 Protein denotes RA
T11833 6626-6655 Protein denotes Amersham, Buckinghamshire, UK
T11832 6419-6422 Entity denotes HCl
T11831 6787-6792 Protein denotes STAT3
T11830 6441-6453 Entity denotes 10% glycerol
T11829 7072-7091 Entity denotes anti-STAT3 antibody
T11828 7240-7248 Protein denotes Antibody
T11827 6371-6376 Entity denotes cells
T10535 5581-5599 Gene_expression denotes β-actin expression
T10534 5289-5294 Protein denotes SOCS1
T10533 4694-4700 Entity denotes (dT)15
T10532 4967-4976 Entity denotes FastStart
T10531 4887-4890 Protein denotes Taq
T10530 4887-4905 Protein denotes Taq DNA polymerase
T10529 4581-4590 Protein denotes total RNA
T10528 4910-4913 Entity denotes DNA
T10527 4977-5000 Entity denotes DNA Master SYBR Green I
T10526 5358-5363 Protein denotes SOCS3
T10525 5521-5526 Protein denotes SOCS3
T10524 4977-5000 Entity denotes DNA Master SYBR Green I
T10523 4368-4381 Protein denotes real-time PCR
T10522 5581-5588 Protein denotes β-actin
T10521 5654-5662 Protein denotes β-actin,
T10520 4445-4448 Protein denotes RNA
T10519 4738-4751 Protein denotes Real-time PCR
T10518 5511-5516 Protein denotes SOCS1
T10517 4359-4363 Protein denotes mRNA
T10516 5454-5461 Protein denotes melting
T10515 5222-5246 Protein denotes β-actin; at 95° C (10 s)
T10514 4420-4462 Entity denotes PB CD4+ T cells using an RNA isolation kit
T10513 5731-5737 Protein denotes SOCS1,
T10512 5806-5812 Protein denotes SOCS3,
T10511 4910-4949 Entity denotes DNA double-strand-specific SYBR Green I
T10510 4596-4646 Protein denotes Molony murine leukemia virus reverse transcriptase
T10509 4420-4422 Protein denotes PB
T9280 4022-4058 Entity denotes culture supernatants of CD4+ T cells
T9279 3981-3985 Protein denotes IL-2
T9278 4189-4218 Protein denotes recombinant cytokine proteins
T9277 4325-4329 Protein denotes IL-2
T9276 4127-4144 Protein denotes cytokine-specific
T9275 3971-3976 Protein denotes IFN-γ
T9274 4004-4009 Protein denotes IFN-γ
T9273 4046-4058 Entity denotes CD4+ T cells
T9272 4189-4218 Protein denotes recombinant cytokine proteins
T9271 4315-4320 Protein denotes IFN-γ
T9270 4014-4018 Protein denotes IL-2
T8978 3346-3364 Gene_expression denotes IL-10R1 expression
T8977 3734-3739 Entity denotes Cells
T8976 3374-3398 Entity denotes of 5 × 105 cells of PBMC
T8975 3684-3723 Protein denotes Santa Cruz Biotechnologies, Santa Cruz,
T8974 3658-3682 Protein denotes IgG1 polyclonal antibody
T8973 3435-3439 Entity denotes PBMC
T8972 3851-3881 Entity denotes 1% FCS/PBS between incubations
T8971 3823-3828 Entity denotes Cells
T8970 3658-3682 Protein denotes IgG1 polyclonal antibody
T8969 3346-3353 Protein denotes IL-10R1
T8968 3745-3798 Protein denotes then incubated with phycoerythrin-conjugated anti-CD4
T8967 3507-3511 Protein denotes IgG1
T8966 3394-3398 Entity denotes PBMC
T8574 2944-2950 Entity denotes T-cell
T8573 3076-3100 Protein denotes absence of 10 ng/ml IL-6
T8572 2966-2974 Protein denotes to IL-10
T8571 2795-2815 Entity denotes Culture supernatants
T8570 3237-3253 Protein denotes of 1 ng/ml IL-10
T8569 2302-2308 Entity denotes T-cell
T8568 2850-2859 Entity denotes cell-free
T8567 2933-2940 Protein denotes of IL-6
T8566 2407-2430 Entity denotes 0.5 ml cell suspensions
T8565 2278-2293 Entity denotes of CD4+ T cells
T8564 2565-2574 Entity denotes The cells
T8563 2294-2325 Protein denotes PB CD4+ T-cell populations were
T8562 3134-3139 Entity denotes Cells
T8561 2895-2909 Protein denotes cytokine assay
T8560 2407-2430 Entity denotes 0.5 ml cell suspensions
T8559 3294-3314 Protein denotes IFN-γ concentrations
T8558 3196-3205 Protein denotes anti-CD28
T8557 2689-2697 Protein denotes of IL-10
T8556 2976-3010 Entity denotes CD4+ T cells from healthy controls
T8555 2506-2527 Protein denotes with 1 μg/ml anti-CD3
T7948 2160-2193 Binding denotes Non-adherent cells were harvested
T7947 1218-1259 Entity denotes Peripheral blood mononuclear cells (PBMC)
T7946 1945-1957 Entity denotes amino acids,
T7945 2160-2178 Entity denotes Non-adherent cells
T7944 1575-1587 Entity denotes CD4+ T cells
T7943 1898-1928 Entity denotes 25 mM HEPES (2 mM L-glutamine,
T7942 1202-1217 Entity denotes of CD4+ T cells
T7941 1492-1507 Protein denotes Miltenyi Biotec
T7940 1664-1666 Protein denotes ST
T7939 1709-1720 Protein denotes collagenase
T7938 1911-1927 Entity denotes 2 mM L-glutamine
T7937 2198-2210 Entity denotes CD4+ T cells
T7936 1781-1800 Entity denotes ST cell suspensions
T7935 1968-1979 Entity denotes penicillin,
T7934 1391-1403 Entity denotes CD4+ T cells
T7933 1254-1258 Entity denotes PBMC
T7932 1607-1609 Protein denotes ST
T7931 1725-1730 Protein denotes DNase
T7930 1988-2006 Entity denotes mg/ml streptomycin
T7929 1423-1449 Entity denotes PBMC by positive selection
T7311 811-814 Protein denotes CRP
T7310 384-421 Entity denotes disease-modifying antirheumatic drugs
T7309 103-105 Protein denotes RA
T7308 589-592 Protein denotes CRP
T7307 352-379 Entity denotes prednisolone (≤ 7.5 mg/day)
T7306 633-650 Protein denotes rheumatoid factor
T7305 569-593 Protein denotes C-reactive protein (CRP)
T7304 510-521 Entity denotes erythrocyte
T7303 658-669 Entity denotes 142 ± 158 U
T7302 1072-1074 Protein denotes ST
T7301 1108-1110 Protein denotes RA
T12071 7789-7798 Entity denotes box plots
T11892 7537-7608 Phosphorylation denotes STAT phosphorylation was compared with the total amount of STAT protein
T11891 6749-6865 Phosphorylation denotes Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,
T11890 6749-6865 Phosphorylation denotes Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,
T11889 6086-6109 Phosphorylation denotes on STAT phosphorylation
T11888 6050-6110 Regulation denotes To examine the effect of serum IL-6 on STAT phosphorylation,
T11887 6749-6757 Entity denotes Tyrosine
T11886 6999-7019 Protein denotes anti-STAT1 antibody,
T11885 6839-6843 Entity denotes Cell
T11884 7056-7061 Protein denotes STAT1
T11883 6391-6397 Protein denotes lysing
T11882 7461-7506 Entity denotes Protein bands were quantified by densitometry
T11881 6939-6951 Entity denotes the membrane
T11880 6414-6422 Entity denotes Tris–HCl
T11879 6424-6428 Entity denotes pH 6
T11878 5891-5903 Entity denotes CD4+ T cells
T11877 6662-6674 Entity denotes the membrane
T11876 6600-6614 Entity denotes nitrocellulose
T11875 5976-5980 Protein denotes IL-6
T11874 6777-6782 Protein denotes STAT1
T11873 6708-6721 Entity denotes Tris-buffered
T11872 7355-7368 Entity denotes Tris-buffered
T11871 7674-7679 Protein denotes STAT1
T11870 6111-6130 Entity denotes normal CD4+ T cells
T11869 6511-6526 Protein denotes protein samples
T11868 7273-7295 Protein denotes horseradish peroxidase
T11867 7133-7138 Protein denotes STAT3
T11866 6072-6085 Protein denotes of serum IL-6
T11865 7056-7070 Entity denotes STAT1 antibody
T11864 7593-7608 Protein denotes of STAT protein
T11863 7596-7600 Protein denotes STAT
T11862 6600-6656 Entity denotes nitrocellulose membranes (Amersham, Buckinghamshire, UK)
T11861 7232-7238 Protein denotes 5% BSA
R4655 T8969 T8978 themeOf IL-10R1,IL-10R1 expression
R5460 T10522 T10535 themeOf β-actin,β-actin expression
R6194 T11831 T11890 themeOf STAT3,"Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,"
R6195 T11838 T11889 themeOf STAT,on STAT phosphorylation
R6196 T11845 T11892 themeOf STAT,STAT phosphorylation was compared with the total amount of STAT protein
R6197 T11866 T11888 causeOf of serum IL-6,"To examine the effect of serum IL-6 on STAT phosphorylation,"
R6198 T11874 T11891 themeOf STAT1,"Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,"
R6199 T11887 T11874 partOf Tyrosine,STAT1
R6200 T11887 T11831 partOf Tyrosine,STAT3
R6201 T11887 T11890 siteOf Tyrosine,"Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,"
R6202 T11887 T11891 siteOf Tyrosine,"Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,"
R6203 T11889 T11888 themeOf on STAT phosphorylation,"To examine the effect of serum IL-6 on STAT phosphorylation,"

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T7911 2267-2268 . denotes .
T12070 7974-7975 . denotes .
T12069 7963-7974 JJ denotes significant
T12068 7952-7962 VBN denotes considered
T12067 7948-7951 VBD denotes was
T12066 7943-7947 CD denotes 0.05
T12065 7941-7942 VBD denotes <
T12064 7939-7940 NNP denotes P
T12063 7937-7938 . denotes .
T12062 7933-7937 NN denotes test
T12061 7928-7932 JJ denotes rank
T12060 7921-7927 VBD denotes signed
T12059 7912-7920 NNP denotes Wilcoxon
T12058 7908-7911 DT denotes the
T12057 7905-7907 CC denotes or
T12056 7900-7904 NN denotes test
T12055 7898-7899 NNP denotes U
T12054 7890-7897 NNP denotes Whitney
T12053 7885-7889 NNP denotes Mann
T12052 7881-7884 DT denotes the
T12051 7878-7880 IN denotes by
T12050 7867-7877 VBN denotes determined
T12049 7863-7866 VBD denotes was
T12048 7856-7862 NNS denotes groups
T12047 7852-7855 CD denotes two
T12046 7844-7851 IN denotes between
T12045 7832-7843 NNS denotes differences
T12044 7829-7831 IN denotes of
T12043 7816-7828 NN denotes significance
T12042 7804-7815 JJ denotes statistical
T12041 7800-7803 DT denotes The
T12040 7798-7799 . denotes .
T12039 7793-7798 NNS denotes plots
T12038 7789-7792 NN denotes box
T12037 7786-7788 CC denotes or
T12036 7781-7785 NN denotes mean
T12035 7777-7780 DT denotes the
T12034 7774-7776 IN denotes of
T12033 7768-7773 NN denotes error
T12032 7759-7767 JJ denotes standard
T12031 7757-7758 NN denotes ±
T12030 7751-7756 NN denotes value
T12029 7746-7750 JJ denotes mean
T12028 7742-7745 DT denotes the
T12027 7739-7741 IN denotes as
T12026 7729-7738 VBN denotes expressed
T12025 7725-7728 VBP denotes are
T12024 7720-7724 NNS denotes Data
T12023 7711-7719 NN denotes analysis
T12022 7699-7710 NNP denotes Statistical
T11758 7696-7697 . denotes .
T11757 7680-7696 NN denotes phosophorylation
T11756 7674-7679 CD denotes STAT1
T11755 7670-7673 IN denotes for
T11754 7662-7669 NN denotes control
T11753 7653-7661 JJ denotes positive
T11752 7651-7652 DT denotes a
T11751 7648-7650 IN denotes as
T11750 7643-7647 VBN denotes used
T11749 7638-7642 VBD denotes were
T11748 7632-7637 NNS denotes cells
T11747 7627-7631 NNP denotes Hela
T11746 7610-7626 JJ denotes IFN-γ-stimulated
T11745 7608-7609 . denotes .
T11744 7601-7608 NN denotes protein
T11743 7596-7600 NNP denotes STAT
T11742 7593-7595 IN denotes of
T11741 7586-7592 NN denotes amount
T11740 7580-7585 JJ denotes total
T11739 7576-7579 DT denotes the
T11738 7571-7575 IN denotes with
T11737 7562-7570 VBN denotes compared
T11736 7558-7561 VBD denotes was
T11735 7542-7557 NN denotes phosphorylation
T11734 7537-7541 NNP denotes STAT
T11733 7533-7536 CC denotes and
T11732 7531-7532 , denotes ,
T11731 7523-7531 NN denotes analysis
T11730 7513-7522 JJ denotes NIH-Image
T11729 7507-7512 VBG denotes using
T11728 7494-7506 NN denotes densitometry
T11727 7491-7493 IN denotes by
T11726 7480-7490 VBN denotes quantified
T11725 7475-7479 VBD denotes were
T11724 7469-7474 NNS denotes bands
T11723 7461-7468 NN denotes Protein
T11722 7459-7460 . denotes .
T11721 7453-7459 NN denotes system
T11720 7435-7452 NN denotes chemiluminescence
T11719 7431-7434 DT denotes the
T11718 7425-7430 VBG denotes using
T11717 7416-7424 VBN denotes revealed
T11716 7412-7415 VBD denotes was
T11715 7408-7411 CC denotes and
T11714 7406-7407 , denotes ,
T11713 7403-7406 NNP denotes BSA
T11712 7401-7402 NN denotes %
T11711 7400-7401 CD denotes 5
T11710 7395-7399 IN denotes with
T11709 7392-7394 CD denotes 20
T11708 7386-7391 CD denotes Tween
T11707 7384-7385 NN denotes %
T11706 7381-7384 CD denotes 0.1
T11705 7376-7380 IN denotes with
T11704 7369-7375 NN denotes saline
T11703 7355-7368 JJ denotes Tris-buffered
T11702 7350-7354 IN denotes with
T11701 7343-7349 CD denotes 1/4000
T11700 7340-7342 IN denotes at
T11699 7332-7339 VBN denotes diluted
T11698 7323-7331 NN denotes antibody
T11697 7319-7322 NNP denotes IgG
T11696 7307-7318 JJ denotes anti-rabbit
T11695 7285-7306 JJ denotes peroxidase-conjugated
T11694 7273-7284 JJ denotes horseradish
T11693 7270-7272 IN denotes by
T11692 7261-7269 VBN denotes detected
T11691 7257-7260 VBD denotes was
T11690 7249-7256 JJ denotes binding
T11689 7240-7248 NN denotes Antibody
T11688 7238-7239 . denotes .
T11687 7235-7238 NNP denotes BSA
T11686 7233-7234 NN denotes %
T11685 7232-7233 CD denotes 5
T11684 7227-7231 IN denotes with
T11683 7224-7226 CD denotes 20
T11682 7218-7223 CD denotes Tween
T11681 7216-7217 NN denotes %
T11680 7213-7216 CD denotes 0.1
T11679 7208-7212 IN denotes with
T11678 7201-7207 NN denotes saline
T11677 7187-7200 JJ denotes Tris-buffered
T11676 7182-7186 IN denotes with
T11675 7175-7181 CD denotes 1/2000
T11674 7172-7174 IN denotes at
T11673 7160-7171 VBN denotes recommended
T11672 7157-7159 IN denotes as
T11671 7149-7156 VBN denotes diluted
T11670 7147-7148 , denotes ,
T11669 7139-7147 NN denotes antibody
T11668 7133-7138 CD denotes STAT3
T11667 7130-7132 IN denotes of
T11666 7126-7129 CD denotes 705
T11665 7117-7125 NN denotes tyrosine
T11664 7097-7116 JJ denotes anti-phosphorylated
T11663 7093-7096 CC denotes and
T11662 7091-7092 , denotes ,
T11661 7083-7091 NN denotes antibody
T11660 7072-7082 JJ denotes anti-STAT3
T11659 7070-7071 , denotes ,
T11658 7062-7070 NN denotes antibody
T11657 7056-7061 CD denotes STAT1
T11656 7053-7055 IN denotes of
T11655 7049-7052 CD denotes 701
T11654 7040-7048 NN denotes tyrosine
T11653 7020-7039 JJ denotes anti-phosphorylated
T11652 7018-7019 , denotes ,
T11651 7010-7018 NN denotes antibody
T11650 6999-7009 JJ denotes anti-STAT1
T11649 6997-6998 -RRB- denotes )
T11648 6994-6997 NNP denotes IgG
T11647 6987-6993 NN denotes rabbit
T11646 6986-6987 -LRB- denotes (
T11645 6975-6985 NNS denotes antibodies
T11644 6971-6974 DT denotes the
T11643 6966-6970 IN denotes with
T11642 6956-6965 VBN denotes incubated
T11641 6952-6955 VBD denotes was
T11640 6943-6951 NN denotes membrane
T11639 6939-6942 DT denotes the
T11638 6937-6938 , denotes ,
T11637 6930-6937 RB denotes Briefly
T11636 6928-6929 . denotes .
T11635 6916-6928 NNS denotes instructions
T11634 6913-6915 POS denotes 's
T11633 6901-6913 NN denotes manufacturer
T11632 6897-6900 DT denotes the
T11631 6894-6896 TO denotes to
T11630 6884-6893 VBG denotes according
T11629 6882-6883 -RRB- denotes )
T11628 6879-6882 NNP denotes USA
T11627 6877-6878 , denotes ,
T11626 6875-6877 NNP denotes MA
T11625 6873-6874 , denotes ,
T11624 6866-6873 NNP denotes Beverly
T11623 6864-6865 , denotes ,
T11622 6854-6864 NNP denotes Technology
T11621 6844-6853 NNP denotes Signaling
T11620 6839-6843 NNP denotes Cell
T11619 6838-6839 -LRB- denotes (
T11618 6833-6837 NNS denotes kits
T11617 6823-6832 JJ denotes available
T11616 6812-6822 JJ denotes commercial
T11615 6806-6811 VBG denotes using
T11614 6797-6805 VBN denotes detected
T11613 6793-6796 VBD denotes was
T11612 6787-6792 CD denotes STAT3
T11611 6783-6786 CC denotes and
T11610 6777-6782 CD denotes STAT1
T11609 6774-6776 IN denotes of
T11608 6758-6773 NN denotes phosphorylation
T11604 6739-6744 CD denotes Tween
T11603 6737-6738 NN denotes %
T11602 6734-6737 CD denotes 0.1
T11601 6729-6733 IN denotes with
T11600 6722-6728 NN denotes saline
T11599 6708-6721 JJ denotes Tris-buffered
T11598 6705-6707 IN denotes in
T11597 6700-6704 NN denotes milk
T11596 6695-6699 VBP denotes skim
T11595 6693-6694 NN denotes %
T11594 6692-6693 CD denotes 5
T11593 6687-6691 IN denotes with
T11592 6679-6686 VBN denotes blocked
T11591 6675-6678 VBD denotes was
T11590 6666-6674 NN denotes membrane
T11589 6662-6665 DT denotes the
T11588 6658-6661 CC denotes and
T11587 6656-6657 , denotes ,
T11586 6655-6656 -RRB- denotes )
T11585 6653-6655 NNP denotes UK
T11584 6651-6652 , denotes ,
T11583 6636-6651 NNP denotes Buckinghamshire
T11582 6634-6635 , denotes ,
T11581 6626-6634 NNP denotes Amersham
T11580 6625-6626 -LRB- denotes (
T11579 6615-6624 NNS denotes membranes
T11578 6600-6614 JJ denotes nitrocellulose
T11577 6597-6599 TO denotes to
T11576 6585-6596 VBN denotes transferred
T11575 6580-6584 VBD denotes were
T11574 6576-6579 CC denotes and
T11573 6571-6575 NNS denotes gels
T11572 6552-6570 JJ denotes SDS-polyacrylamide
T11571 6550-6551 NN denotes %
T11570 6548-6550 CD denotes 10
T11569 6545-6547 IN denotes on
T11568 6532-6544 VBN denotes fractionated
T11567 6527-6531 VBD denotes were
T11566 6519-6526 NNS denotes samples
T11565 6511-6518 NN denotes protein
T11564 6508-6510 NN denotes μl
T11563 6505-6507 CD denotes 20
T11562 6500-6504 RB denotes Then
T11561 6498-6499 . denotes .
T11560 6497-6498 -RRB- denotes )
T11559 6493-6497 JJ denotes blue
T11558 6482-6492 NN denotes bromphenol
T11557 6480-6481 NN denotes %
T11556 6477-6480 CD denotes 0.1
T11555 6475-6476 , denotes ,
T11554 6461-6475 NN denotes dithiothreitol
T11553 6458-6460 NN denotes mM
T11552 6455-6457 CD denotes 50
T11551 6453-6454 , denotes ,
T11550 6445-6453 NN denotes glycerol
T11549 6443-6444 NN denotes %
T11548 6441-6443 CD denotes 10
T11547 6439-6440 , denotes ,
T11546 6436-6439 NNP denotes SDS
T11545 6434-6435 NN denotes %
T11544 6433-6434 CD denotes 2
T11543 6431-6432 , denotes ,
T11542 6430-6431 NNP denotes ]
T11541 6427-6430 CD denotes 6.8
T11540 6424-6426 NNP denotes pH
T11539 6423-6424 NNP denotes [
T11538 6419-6422 NNP denotes HCl
T11537 6414-6418 NNP denotes Tris
T11536 6411-6413 NNP denotes mM
T11535 6406-6410 CD denotes 62.5
T11534 6405-6406 -LRB- denotes (
T11533 6398-6404 NN denotes buffer
T11532 6391-6397 VBG denotes lysing
T11531 6387-6390 NNP denotes SDS
T11530 6384-6386 NN denotes μl
T11529 6380-6383 CD denotes 100
T11528 6377-6379 IN denotes in
T11527 6371-6376 NNS denotes cells
T11526 6363-6370 VBG denotes placing
T11525 6360-6362 IN denotes by
T11524 6351-6359 VBN denotes prepared
T11523 6346-6350 VBD denotes were
T11522 6338-6345 NNS denotes lysates
T11521 6333-6337 NN denotes cell
T11520 6327-6332 JJ denotes Whole
T11519 6325-6326 . denotes .
T11518 6324-6325 -RRB- denotes )
T11517 6318-6324 NNP denotes Techne
T11516 6317-6318 -LRB- denotes (
T11515 6313-6316 NNP denotes IgG
T11514 6308-6312 JJ denotes goat
T11513 6300-6307 VB denotes control
T11512 6297-6299 CC denotes or
T11511 6295-6296 -RRB- denotes )
T11510 6292-6295 NNP denotes USA
T11509 6290-6291 , denotes ,
T11508 6288-6290 NNP denotes NJ
T11507 6286-6287 , denotes ,
T11506 6277-6286 NNP denotes Princeton
T11505 6275-6276 , denotes ,
T11504 6269-6275 NNP denotes Techne
T11503 6267-6268 : denotes ;
T11502 6264-6267 NNP denotes IgG
T11501 6263-6264 -LRB- denotes (
T11500 6254-6262 NN denotes antibody
T11499 6243-6253 JJ denotes polyclonal
T11498 6233-6242 JJ denotes anti-IL-6
T11497 6228-6232 NN denotes goat
T11496 6215-6227 VBG denotes neutralizing
T11495 6212-6214 NN denotes ml
T11494 6211-6212 NN denotes /
T11493 6209-6211 NN denotes μg
T11492 6206-6208 CD denotes 40
T11491 6201-6205 IN denotes with
T11490 6194-6200 NN denotes medium
T11489 6186-6193 NN denotes culture
T11488 6183-6185 IN denotes in
T11487 6177-6182 NN denotes serum
T11486 6174-6176 NNP denotes RA
T11485 6167-6173 JJ denotes active
T11484 6165-6166 NN denotes %
T11483 6163-6165 CD denotes 30
T11482 6158-6162 IN denotes with
T11481 6154-6157 NN denotes min
T11480 6151-6153 CD denotes 20
T11479 6147-6150 IN denotes for
T11478 6136-6146 VBN denotes stimulated
T11477 6131-6135 VBD denotes were
T11476 6125-6130 NNS denotes cells
T11475 6123-6124 NNP denotes T
T11474 6121-6122 CD denotes +
T11473 6118-6121 NNP denotes CD4
T11472 6111-6117 JJ denotes normal
T11471 6109-6110 , denotes ,
T11470 6094-6109 NN denotes phosphorylation
T11469 6089-6093 NNP denotes STAT
T11468 6086-6088 IN denotes on
T11467 6081-6085 NN denotes IL-6
T11466 6075-6080 NN denotes serum
T11465 6072-6074 IN denotes of
T11464 6065-6071 NN denotes effect
T11463 6061-6064 DT denotes the
T11462 6053-6060 VB denotes examine
T11461 6050-6052 TO denotes To
T11460 6048-6049 . denotes .
T11459 6045-6048 NNP denotes FCS
T11458 6043-6044 NN denotes %
T11457 6041-6043 CD denotes 10
T11456 6036-6040 IN denotes with
T11455 6029-6035 NN denotes medium
T11454 6021-6028 NN denotes culture
T11453 6018-6020 JJ denotes ml
T11452 6014-6017 CD denotes 0.5
T11451 6011-6013 IN denotes in
T11450 6005-6010 NNS denotes cells
T11449 6001-6004 CD denotes 105
T11448 5999-6000 CD denotes ×
T11447 5997-5998 CD denotes 5
T11446 5994-5996 IN denotes of
T11445 5986-5993 NN denotes density
T11444 5984-5985 DT denotes a
T11443 5981-5983 IN denotes at
T11442 5976-5980 NN denotes IL-6
T11441 5972-5975 CC denotes and
T11440 5966-5971 NN denotes IL-10
T11439 5963-5965 IN denotes of
T11438 5948-5962 NNS denotes concentrations
T11437 5938-5947 VBN denotes indicated
T11436 5934-5937 DT denotes the
T11435 5931-5933 IN denotes by
T11434 5927-5930 NN denotes min
T11433 5924-5926 CD denotes 20
T11432 5920-5923 IN denotes for
T11431 5909-5919 VBN denotes stimulated
T11430 5904-5908 VBD denotes were
T11429 5898-5903 NNS denotes cells
T11428 5896-5897 NNP denotes T
T11427 5894-5895 NNP denotes +
T11426 5891-5894 NNP denotes CD4
T11425 5882-5890 NN denotes analysis
T11424 5877-5881 NN denotes blot
T11423 5869-5876 JJ denotes Western
T11607 6749-6757 NNP denotes Tyrosine
T11606 6747-6748 . denotes .
T11605 6745-6747 CD denotes 20
T10468 5866-5867 . denotes .
T10467 5865-5866 '' denotes '
T10466 5845-5865 NN denotes AGGGGCCGGCTCAACACC-3
T10465 5844-5845 : denotes -
T10464 5843-5844 '' denotes '
T10463 5842-5843 CD denotes 5
T10462 5838-5841 CC denotes and
T10461 5836-5837 '' denotes '
T10460 5816-5836 NN denotes CCCGCCGGCACCTTTCTG-3
T10459 5815-5816 : denotes -
T10458 5814-5815 '' denotes '
T10457 5813-5814 CD denotes 5
T10456 5811-5812 , denotes ,
T10455 5806-5811 NNP denotes SOCS3
T10454 5802-5805 IN denotes for
T10453 5798-5801 CC denotes and
T10452 5796-5797 : denotes ;
T10451 5794-5795 '' denotes '
T10450 5772-5794 NN denotes GCACAGCAGAAAAATAAAGC-3
T10449 5771-5772 : denotes -
T10448 5770-5771 '' denotes '
T10447 5769-5770 CD denotes 5
T10446 5765-5768 CC denotes and
T10445 5763-5764 '' denotes '
T10444 5741-5763 NN denotes AGACCCCTTCTCACCTCTTG-3
T10443 5740-5741 : denotes -
T10442 5739-5740 '' denotes '
T10441 5738-5739 CD denotes 5
T10440 5736-5737 , denotes ,
T10439 5731-5736 NNP denotes SOCS1
T10438 5727-5730 IN denotes for
T10437 5725-5726 : denotes ;
T10436 5723-5724 '' denotes '
T10435 5697-5723 NN denotes CTCCTTAATGTCACGCACGATTTC-3
T10434 5696-5697 : denotes -
T10433 5695-5696 '' denotes '
T10432 5694-5695 CD denotes 5
T10431 5690-5693 CC denotes and
T10430 5688-5689 '' denotes '
T10429 5666-5688 NN denotes GTGGGGCGCCCCAGGCACCA-3
T10428 5665-5666 : denotes -
T10427 5664-5665 '' denotes '
T10426 5663-5664 CD denotes 5
T10425 5661-5662 , denotes ,
T10424 5654-5661 NN denotes β-actin
T10423 5650-5653 IN denotes for
T10422 5648-5649 : denotes :
T10421 5641-5648 VBZ denotes follows
T10420 5638-5640 IN denotes as
T10419 5633-5637 VBD denotes were
T10418 5625-5632 NNS denotes primers
T10417 5617-5624 VB denotes reverse
T10416 5613-5616 CC denotes and
T10415 5605-5612 JJ denotes forward
T10414 5601-5604 DT denotes The
T10413 5599-5600 . denotes .
T10412 5589-5599 NN denotes expression
T10411 5581-5588 VB denotes β-actin
T10410 5578-5580 TO denotes to
T10409 5569-5577 JJ denotes relative
T10408 5557-5568 VBG denotes normalizing
T10407 5554-5556 IN denotes by
T10406 5543-5553 VBN denotes determined
T10405 5538-5542 VBD denotes were
T10404 5527-5537 NN denotes expression
T10403 5521-5526 CD denotes SOCS3
T10402 5517-5520 CC denotes and
T10401 5511-5516 CD denotes SOCS1
T10400 5508-5510 IN denotes of
T10399 5501-5507 NNS denotes levels
T10398 5497-5500 DT denotes The
T10397 5495-5496 . denotes .
T10396 5486-5495 VBN denotes performed
T10395 5481-5485 RB denotes then
T10394 5477-5480 VBD denotes was
T10393 5468-5476 NN denotes analysis
T10392 5462-5467 NN denotes curve
T10391 5454-5461 NN denotes melting
T10390 5452-5453 DT denotes a
T10389 5448-5451 CC denotes and
T10388 5446-5447 , denotes ,
T10387 5438-5446 VBN denotes obtained
T10386 5433-5437 VBD denotes were
T10385 5426-5432 NN denotes number
T10384 5420-5425 NN denotes cycle
T10383 5413-5419 CC denotes versus
T10382 5406-5412 NNS denotes values
T10381 5393-5405 NN denotes fluorescence
T10380 5389-5392 DT denotes the
T10379 5386-5388 IN denotes of
T10378 5379-5385 NNS denotes curves
T10377 5365-5378 NNP denotes Amplification
T10376 5363-5364 . denotes .
T10375 5358-5363 NNP denotes SOCS3
T10374 5354-5357 IN denotes for
T10373 5352-5353 -RRB- denotes )
T10372 5351-5352 PRP denotes s
T10371 5348-5350 CD denotes 15
T10370 5347-5348 -LRB- denotes (
T10369 5345-5346 NNP denotes C
T10368 5343-5344 CD denotes °
T10367 5341-5343 CD denotes 72
T10366 5338-5340 IN denotes at
T10365 5334-5337 CC denotes and
T10364 5332-5333 , denotes ,
T10363 5331-5332 -RRB- denotes )
T10362 5330-5331 PRP denotes s
T10361 5327-5329 CD denotes 15
T10360 5326-5327 -LRB- denotes (
T10359 5324-5325 NNP denotes C
T10358 5322-5323 CD denotes °
T10357 5320-5322 CD denotes 68
T10356 5317-5319 IN denotes at
T10355 5315-5316 , denotes ,
T10354 5314-5315 -RRB- denotes )
T10353 5313-5314 PRP denotes s
T10352 5310-5312 CD denotes 10
T10351 5309-5310 -LRB- denotes (
T10350 5307-5308 NNP denotes C
T10349 5305-5306 CD denotes °
T10348 5303-5305 CD denotes 96
T10347 5300-5302 IN denotes at
T10346 5296-5299 CC denotes and
T10345 5294-5295 : denotes ;
T10344 5289-5294 CD denotes SOCS1
T10343 5285-5288 IN denotes for
T10342 5283-5284 -RRB- denotes )
T10341 5282-5283 PRP denotes s
T10340 5279-5281 CD denotes 10
T10339 5278-5279 -LRB- denotes (
T10338 5276-5277 NNP denotes C
T10337 5274-5275 CD denotes °
T10336 5272-5274 CD denotes 72
T10335 5269-5271 IN denotes at
T10334 5265-5268 CC denotes and
T10333 5263-5264 , denotes ,
T10332 5262-5263 -RRB- denotes )
T10331 5261-5262 PRP denotes s
T10330 5258-5260 CD denotes 15
T10329 5257-5258 -LRB- denotes (
T10328 5255-5256 NNP denotes C
T10327 5253-5254 CD denotes °
T10326 5251-5253 CD denotes 62
T10325 5248-5250 IN denotes at
T10324 5246-5247 , denotes ,
T10323 5245-5246 -RRB- denotes )
T10322 5244-5245 PRP denotes s
T10321 5241-5243 CD denotes 10
T10320 5240-5241 -LRB- denotes (
T10319 5238-5239 NNP denotes C
T10318 5236-5237 CD denotes °
T10317 5234-5236 CD denotes 95
T10316 5231-5233 IN denotes at
T10315 5229-5230 : denotes ;
T10314 5222-5229 NN denotes β-actin
T10313 5218-5221 IN denotes for
T10312 5216-5217 -RRB- denotes )
T10311 5215-5216 PRP denotes s
T10310 5212-5214 CD denotes 22
T10309 5211-5212 -LRB- denotes (
T10308 5209-5210 NNP denotes C
T10307 5207-5208 NN denotes °
T10306 5205-5207 CD denotes 72
T10305 5201-5204 CC denotes and
T10304 5199-5200 , denotes ,
T10303 5198-5199 -RRB- denotes )
T10302 5197-5198 PRP denotes s
T10301 5194-5196 CD denotes 15
T10300 5193-5194 -LRB- denotes (
T10299 5191-5192 NNP denotes C
T10298 5189-5190 CD denotes °
T10297 5187-5189 CD denotes 65
T10296 5184-5186 IN denotes at
T10295 5182-5183 , denotes ,
T10294 5181-5182 -RRB- denotes )
T10293 5180-5181 PRP denotes s
T10292 5177-5179 CD denotes 10
T10291 5176-5177 -LRB- denotes (
T10290 5174-5175 NNP denotes C
T10289 5172-5173 CD denotes °
T10288 5170-5172 CD denotes 95
T10287 5167-5169 IN denotes at
T10286 5165-5166 : denotes :
T10285 5159-5165 NNS denotes cycles
T10284 5156-5158 CD denotes 50
T10283 5153-5155 CD denotes 40
T10282 5149-5152 IN denotes for
T10281 5139-5148 VBN denotes amplified
T10280 5134-5138 RB denotes then
T10279 5129-5133 VBD denotes were
T10278 5125-5128 CC denotes and
T10277 5123-5124 , denotes ,
T10276 5120-5123 NN denotes min
T10275 5117-5119 CD denotes 10
T10274 5113-5116 IN denotes for
T10273 5111-5112 NNP denotes C
T10272 5109-5110 CD denotes °
T10271 5107-5109 CD denotes 95
T10270 5104-5106 IN denotes at
T10269 5094-5103 VBN denotes denatured
T10268 5089-5093 VBD denotes were
T10267 5081-5088 NNS denotes samples
T10266 5076-5080 NNP denotes cDNA
T10265 5072-5075 DT denotes The
T10264 5070-5071 . denotes .
T10228 4846-4857 NNS denotes capillaries
T10227 4840-4845 NN denotes glass
T10226 4837-4839 IN denotes in
T10225 4835-4836 -RRB- denotes )
T10224 4828-4835 NNP denotes Germany
T10223 4826-4827 , denotes ,
T10222 4818-4826 NNP denotes Penzberg
T10221 4816-4817 , denotes ,
T10220 4805-4816 NNPS denotes Diagnostics
T10219 4799-4804 NNP denotes Roche
T10218 4798-4799 -LRB- denotes (
T10217 4787-4797 NNP denotes Instrument
T10216 4775-4786 NNP denotes LightCycler
T10215 4771-4774 DT denotes the
T10214 4766-4770 IN denotes with
T10213 4756-4765 VBN denotes performed
T10212 4752-4755 VBD denotes was
T10211 4748-4751 NNP denotes PCR
T10210 4738-4747 JJ denotes Real-time
T10209 4736-4737 . denotes .
T10208 4735-4736 -RRB- denotes )
T10207 4732-4735 NNP denotes USA
T10206 4730-4731 , denotes ,
T10205 4728-4730 NNP denotes WI
T10204 4726-4727 , denotes ,
T10203 4719-4726 NNP denotes Madison
T10202 4717-4718 , denotes ,
T10201 4710-4717 NNP denotes Promega
T10200 4709-4710 -LRB- denotes (
T10199 4701-4708 NNS denotes primers
T10198 4698-4700 CD denotes 15
T10197 4697-4698 -RRB- denotes )
T10196 4695-4697 NN denotes dT
T10195 4694-4695 -LRB- denotes (
T10194 4693-4694 : denotes -
T10193 4688-4693 SYM denotes oligo
T10192 4684-4687 CC denotes and
T10191 4682-4683 -RRB- denotes )
T10190 4679-4682 NNP denotes USA
T10189 4677-4678 , denotes ,
T10188 4675-4677 NNP denotes OH
T10187 4673-4674 , denotes ,
T10186 4664-4673 NNP denotes Cleveland
T10185 4662-4663 , denotes ,
T10184 4651-4662 NNP denotes Biochemical
T10183 4648-4650 NNP denotes US
T10182 4647-4648 -LRB- denotes (
T10181 4633-4646 NN denotes transcriptase
T10180 4625-4632 VB denotes reverse
T10179 4619-4624 NN denotes virus
T10178 4610-4618 NN denotes leukemia
T10177 4603-4609 NN denotes murine
T10176 4596-4602 NNP denotes Molony
T10175 4591-4595 IN denotes with
T10174 4587-4590 NNP denotes RNA
T10173 4581-4586 JJ denotes total
T10172 4576-4580 IN denotes from
T10171 4564-4575 VBN denotes synthesized
T10170 4560-4563 VBD denotes was
T10169 4555-4559 NNP denotes cDNA
T10168 4553-4554 . denotes .
T10167 4541-4553 NNS denotes instructions
T10166 4538-4540 POS denotes 's
T10165 4526-4538 NN denotes manufacturer
T10164 4522-4525 DT denotes the
T10163 4519-4521 TO denotes to
T10162 4509-4518 VBG denotes according
T10161 4507-4508 , denotes ,
T10160 4506-4507 -RRB- denotes )
T10159 4503-4506 NNP denotes USA
T10158 4501-4502 , denotes ,
T10157 4499-4501 NNP denotes CA
T10156 4497-4498 , denotes ,
T10155 4489-4497 NNP denotes Valencia
T10154 4487-4488 , denotes ,
T10153 4481-4487 NNP denotes Qiagen
T10152 4479-4480 : denotes ;
T10151 4476-4479 NN denotes kit
T10150 4471-4475 NNP denotes Mini
T10149 4464-4470 NNP denotes RNeasy
T10148 4463-4464 -LRB- denotes (
T10147 4459-4462 NN denotes kit
T10146 4449-4458 NN denotes isolation
T10145 4445-4448 NNP denotes RNA
T10144 4442-4444 DT denotes an
T10143 4436-4441 VBG denotes using
T10142 4430-4435 NNS denotes cells
T10141 4428-4429 NNP denotes T
T10140 4426-4427 VBD denotes +
T10139 4423-4426 NNP denotes CD4
T10138 4420-4422 NNP denotes PB
T10137 4415-4419 IN denotes from
T10136 4405-4414 VBN denotes extracted
T10135 4401-4404 VBD denotes was
T10134 4397-4400 NNP denotes RNA
T10133 4388-4396 JJ denotes cellular
T10132 4382-4387 NNP denotes Total
T10131 4378-4381 NNP denotes PCR
T10130 4368-4377 JJ denotes real-time
T10129 4364-4367 CC denotes and
T10128 4359-4363 NNP denotes mRNA
T10127 4356-4358 IN denotes of
T10126 4346-4355 NN denotes Isolation
T10263 5061-5070 NNS denotes dilutions
T10262 5056-5060 NNP denotes cDNA
T10261 5053-5055 TO denotes to
T10260 5047-5052 VBN denotes added
T10259 5042-5046 VBD denotes were
T10258 5034-5041 NNS denotes primers
T10257 5025-5033 JJ denotes specific
T10256 5021-5024 CC denotes and
T10255 5019-5020 -RRB- denotes )
T10254 5008-5019 NNPS denotes Diagnostics
T10253 5002-5007 NNP denotes Roche
T10252 5000-5001 : denotes ;
T10251 4999-5000 PRP denotes I
T10250 4993-4998 NNP denotes Green
T10249 4988-4992 NNP denotes SYBR
T10248 4981-4987 NNP denotes Master
T10247 4977-4980 NNP denotes DNA
T10246 4967-4976 NNP denotes FastStart
T10245 4955-4966 NNP denotes Lightcycler
T10244 4954-4955 -LRB- denotes (
T10243 4950-4953 VBP denotes dye
T10242 4948-4949 PRP denotes I
T10241 4942-4947 NNP denotes Green
T10240 4937-4941 NNP denotes SYBR
T10239 4914-4936 JJ denotes double-strand-specific
T10238 4910-4913 NN denotes DNA
T10237 4906-4909 CC denotes and
T10236 4895-4905 NN denotes polymerase
T10235 4891-4894 NNP denotes DNA
T10234 4887-4890 NNP denotes Taq
T10233 4876-4886 VBG denotes containing
T10232 4872-4875 NN denotes mix
T10231 4863-4871 NN denotes reaction
T10230 4859-4862 DT denotes The
T10229 4857-4858 . denotes .
T9247 4343-4344 . denotes .
T9246 4338-4343 NN denotes pg/ml
T9245 4335-4337 CD denotes 15
T9244 4330-4334 VBD denotes were
T9243 4325-4329 NNP denotes IL-2
T9242 4321-4324 CC denotes and
T9241 4315-4320 NNP denotes IFN-γ
T9240 4311-4314 IN denotes for
T9239 4304-4310 NNS denotes limits
T9238 4294-4303 NN denotes detection
T9237 4290-4293 DT denotes The
T9236 4288-4289 . denotes .
T9235 4280-4288 NN denotes protocol
T9234 4277-4279 POS denotes 's
T9233 4265-4277 NN denotes manufacturer
T9232 4261-4264 DT denotes the
T9231 4258-4260 TO denotes to
T9230 4248-4257 VBG denotes according
T9229 4246-4247 , denotes ,
T9228 4245-4246 -RRB- denotes )
T9227 4236-4245 NNP denotes Dickinson
T9226 4229-4235 NNP denotes Becton
T9225 4224-4228 IN denotes from
T9224 4220-4223 DT denotes all
T8960 3952-3953 . denotes .
T8959 3951-3952 -RRB- denotes )
T8958 3942-3951 NNP denotes Dickinson
T8957 3935-3941 NNP denotes Becton
T8956 3934-3935 -LRB- denotes (
T8955 3924-3933 NN denotes cytometer
T8954 3919-3923 NN denotes flow
T8953 3911-3918 JJ denotes FACScan
T8952 3909-3910 DT denotes a
T8951 3906-3908 IN denotes on
T8950 3896-3905 VBN denotes performed
T8949 3892-3895 VBD denotes was
T8948 3883-3891 NN denotes Analysis
T8947 3881-3882 . denotes .
T8946 3870-3881 NNS denotes incubations
T8945 3862-3869 IN denotes between
T8944 3854-3861 NN denotes FCS/PBS
T8943 3852-3853 NN denotes %
T8942 3851-3852 CD denotes 1
T8941 3846-3850 IN denotes with
T8940 3841-3845 RB denotes well
T8939 3834-3840 VBN denotes washed
T8938 3829-3833 VBD denotes were
T8937 3823-3828 NNS denotes Cells
T8936 3821-3822 . denotes .
T8935 3820-3821 -RRB- denotes )
T8934 3811-3820 NNP denotes Dickinson
T8933 3804-3810 NNP denotes Becton
T8932 3803-3804 -LRB- denotes (
T8931 3799-3802 NNP denotes mAb
T8930 3790-3798 JJ denotes anti-CD4
T8929 3765-3789 JJ denotes phycoerythrin-conjugated
T8928 3760-3764 IN denotes with
T8927 3750-3759 VBN denotes incubated
T8926 3745-3749 RB denotes then
T8925 3740-3744 VBD denotes were
T8924 3734-3739 NNS denotes Cells
T8923 3732-3733 . denotes .
T8922 3731-3732 -RRB- denotes )
T8921 3728-3731 NNP denotes USA
T8920 3726-3727 , denotes ,
T8919 3724-3726 NNP denotes CA
T8918 3722-3723 , denotes ,
T8917 3718-3722 NNP denotes Cruz
T8916 3712-3717 NNP denotes Santa
T8915 3710-3711 , denotes ,
T8914 3695-3710 NNPS denotes Biotechnologies
T8913 3690-3694 NNP denotes Cruz
T8912 3684-3689 NNP denotes Santa
T8911 3683-3684 -LRB- denotes (
T8910 3674-3682 NN denotes antibody
T8909 3663-3673 NN denotes polyclonal
T8908 3658-3662 JJ denotes IgG1
T8907 3647-3657 JJ denotes anti-mouse
T8906 3642-3646 NN denotes goat
T8895 3572-3579 NN denotes control
T8894 3556-3571 JJ denotes isotype-matched
T8893 3551-3555 IN denotes with
T8892 3548-3550 CC denotes or
T8891 3546-3547 -RRB- denotes )
T8890 3543-3546 NNP denotes USA
T8889 3541-3542 , denotes ,
T8888 3539-3541 NNP denotes MN
T8887 3537-3538 , denotes ,
T8886 3526-3537 NNP denotes Minneapolis
T8885 3524-3525 , denotes ,
T8884 3517-3524 NNS denotes systems
T8883 3513-3516 NNP denotes R&D
T8882 3511-3512 : denotes ;
T8881 3507-3511 NNP denotes IgG1
T8880 3506-3507 -LRB- denotes (
T8879 3502-3505 NNP denotes mAb
T8878 3489-3501 JJ denotes anti-IL-10R1
T8877 3486-3488 IN denotes of
T8876 3471-3485 NNS denotes concentrations
T8875 3460-3470 VBG denotes saturating
T8874 3455-3459 IN denotes with
T8873 3445-3454 VBN denotes incubated
T8872 3440-3444 VBD denotes were
T8871 3435-3439 NNP denotes PBMC
T8870 3433-3434 . denotes .
T8869 3430-3433 NNP denotes FCS
T8868 3428-3429 NN denotes %
T8867 3427-3428 CD denotes 1
T8866 3422-3426 IN denotes with
T8865 3418-3421 NNP denotes PBS
T8864 3415-3417 IN denotes in
T8863 3403-3414 VBN denotes resuspended
T8862 3399-3402 VBD denotes was
T8861 3394-3398 NNP denotes PBMC
T8860 3391-3393 IN denotes of
T8859 3385-3390 NNS denotes cells
T8858 3381-3384 CD denotes 105
T8857 3379-3380 CD denotes ×
T8856 3377-3378 CD denotes 5
T8855 3374-3376 IN denotes of
T8854 3367-3373 NN denotes sample
T8853 3365-3366 DT denotes A
T8852 3354-3364 NN denotes expression
T8851 3346-3353 NNP denotes IL-10R1
T8850 3342-3345 IN denotes for
T8849 3333-3341 NN denotes analysis
T8848 3322-3332 JJ denotes cytometric
T8847 3317-3321 NN denotes Flow
T9223 4219-4220 -LRB- denotes (
T9222 4210-4218 NNS denotes proteins
T9221 4201-4209 NN denotes cytokine
T9220 4189-4200 JJ denotes recombinant
T9219 4185-4188 CC denotes and
T9218 4181-4184 NNP denotes mAb
T9217 4171-4180 NN denotes detection
T9216 4158-4170 JJ denotes biotinylated
T9215 4153-4157 IN denotes with
T9214 4145-4152 NN denotes capture
T9213 4127-4144 JJ denotes cytokine-specific
T9212 4121-4126 VBG denotes using
T9211 4115-4120 NNP denotes ELISA
T9210 4106-4114 NN denotes sandwich
T9209 4093-4105 JJ denotes quantitative
T9208 4089-4092 DT denotes the
T9207 4086-4088 IN denotes by
T9206 4076-4085 VB denotes duplicate
T9205 4073-4075 IN denotes in
T9204 4064-4072 VBN denotes measured
T9203 4059-4063 VBD denotes were
T9202 4053-4058 NNS denotes cells
T9201 4051-4052 NNP denotes T
T9200 4049-4050 NNP denotes +
T9199 4046-4049 NNP denotes CD4
T9198 4043-4045 IN denotes of
T9197 4030-4042 NNS denotes supernatants
T9196 4022-4029 NN denotes culture
T9195 4019-4021 IN denotes in
T9194 4014-4018 NNP denotes IL-2
T9193 4010-4013 CC denotes and
T9192 4004-4009 NNP denotes IFN-γ
T9191 4001-4003 IN denotes of
T9190 3986-4000 NNPS denotes Concentrations
T9189 3981-3985 NNP denotes IL-2
T9188 3977-3980 CC denotes and
T9187 3971-3976 NNP denotes IFN-γ
T9186 3967-3970 IN denotes for
T9185 3955-3966 NNP denotes Immunoassay
T8522 3314-3315 . denotes .
T8521 3300-3314 NNS denotes concentrations
T8520 3294-3299 JJ denotes IFN-γ
T8519 3290-3293 IN denotes for
T8518 3281-3289 VBN denotes measured
T8517 3276-3280 VBD denotes were
T8516 3263-3275 NNS denotes supernatants
T8515 3255-3262 NNP denotes Culture
T8514 3253-3254 . denotes .
T8513 3248-3253 NN denotes IL-10
T8512 3242-3247 NN denotes ng/ml
T8511 3240-3241 CD denotes 1
T8510 3237-3239 IN denotes of
T8509 3229-3236 NN denotes absence
T8508 3226-3228 CC denotes or
T8507 3217-3225 NN denotes presence
T8506 3213-3216 DT denotes the
T8505 3210-3212 IN denotes in
T8504 3206-3209 NN denotes mAb
T8503 3196-3205 JJ denotes anti-CD28
T8502 3192-3195 CC denotes and
T8501 3188-3191 NNP denotes mAb
T8500 3179-3187 JJ denotes anti-CD3
T8499 3174-3178 IN denotes with
T8498 3168-3173 NNS denotes hours
T8497 3165-3167 CD denotes 36
T8496 3161-3164 IN denotes for
T8495 3150-3160 VBN denotes stimulated
T8494 3145-3149 RB denotes then
T8493 3140-3144 VBD denotes were
T8905 3626-3641 JJ denotes FITC-conjugated
T8904 3621-3625 IN denotes with
T8903 3610-3620 NN denotes incubation
T8902 3607-3609 IN denotes by
T8901 3598-3606 VBN denotes followed
T8900 3596-3597 , denotes ,
T8899 3595-3596 -RRB- denotes )
T8898 3585-3595 NNP denotes Immunotech
T8897 3584-3585 -LRB- denotes (
T8896 3580-3583 NNP denotes mAb
T8492 3134-3139 NNS denotes Cells
T8491 3132-3133 . denotes .
T8490 3127-3132 NNS denotes hours
T8489 3124-3126 CD denotes 36
T8488 3120-3123 IN denotes for
T8487 3118-3119 -RRB- denotes )
T8486 3109-3118 NNP denotes Dickinson
T8485 3102-3108 NNP denotes Becton
T8484 3101-3102 -LRB- denotes (
T8483 3096-3100 NN denotes IL-6
T8482 3090-3095 NN denotes ng/ml
T8481 3087-3089 CD denotes 10
T8480 3084-3086 IN denotes of
T8479 3076-3083 NN denotes absence
T8478 3073-3075 CC denotes or
T8477 3064-3072 NN denotes presence
T8476 3060-3063 DT denotes the
T8475 3057-3059 IN denotes in
T8474 3053-3056 NNP denotes FCS
T8473 3051-3052 NN denotes %
T8472 3049-3051 CD denotes 10
T8471 3044-3048 IN denotes with
T8470 3037-3043 NN denotes medium
T8469 3029-3036 NN denotes culture
T8468 3026-3028 IN denotes in
T8467 3016-3025 VBN denotes incubated
T8466 3011-3015 VBD denotes were
T8465 3002-3010 NNS denotes controls
T8464 2994-3001 JJ denotes healthy
T8463 2989-2993 IN denotes from
T8462 2983-2988 NNS denotes cells
T8461 2981-2982 NNP denotes T
T7910 2258-2267 VBN denotes described
T7909 2250-2257 RB denotes already
T7908 2247-2249 IN denotes as
T7907 2237-2246 NN denotes selection
T7906 2228-2236 JJ denotes positive
T7905 2225-2227 IN denotes by
T7904 2216-2224 VBN denotes purified
T7903 2211-2215 VBD denotes were
T7902 2205-2210 NNS denotes cells
T7901 2203-2204 NNP denotes T
T7900 2201-2202 CD denotes +
T7899 2198-2201 NNP denotes CD4
T7898 2194-2197 CC denotes and
T7897 2184-2193 VBN denotes harvested
T7896 2179-2183 VBD denotes were
T7895 2173-2178 NNS denotes cells
T7894 2160-2172 JJ denotes Non-adherent
T7893 2158-2159 . denotes .
T7892 2155-2158 NN denotes min
T7891 2152-2154 CD denotes 45
T7890 2148-2151 IN denotes for
T7889 2146-2147 -RRB- denotes )
T7888 2143-2146 NNP denotes USA
T7887 2141-2142 , denotes ,
T7886 2139-2141 NNP denotes MA
T7885 2137-2138 , denotes ,
T7884 2128-2137 NNP denotes Cambridge
T7883 2126-2127 , denotes ,
T7882 2120-2126 NNP denotes Coster
T7881 2119-2120 -LRB- denotes (
T7880 2112-2118 NNS denotes plates
T7879 2103-2111 JJ denotes six-well
T7878 2100-2102 IN denotes in
T7877 2098-2099 NNP denotes C
T8460 2979-2980 NNP denotes +
T8459 2976-2979 NNP denotes CD4
T8458 2974-2975 , denotes ,
T8457 2969-2974 NNP denotes IL-10
T8456 2966-2968 TO denotes to
T8455 2951-2965 NN denotes responsiveness
T8454 2944-2950 NN denotes T-cell
T8453 2941-2943 IN denotes on
T8452 2936-2940 NN denotes IL-6
T8451 2933-2935 IN denotes of
T8450 2926-2932 NN denotes effect
T8449 2922-2925 DT denotes the
T8448 2914-2921 VB denotes examine
T8447 2911-2913 TO denotes To
T8446 2909-2910 . denotes .
T8445 2904-2909 NN denotes assay
T8444 2895-2903 JJ denotes cytokine
T8443 2889-2894 IN denotes until
T8442 2887-2888 NNP denotes C
T8441 2886-2887 CD denotes °
T8440 2883-2886 CD denotes -30
T8439 2880-2882 IN denotes at
T8438 2873-2879 VBN denotes stored
T8437 2868-2872 VBD denotes were
T8436 2860-2867 NNS denotes samples
T8435 2850-2859 JJ denotes cell-free
T8434 2846-2849 CC denotes and
T8433 2840-2845 RB denotes later
T8432 2834-2839 NNS denotes hours
T8431 2831-2833 CD denotes 36
T8430 2821-2830 VBN denotes collected
T8429 2816-2820 VBD denotes were
T8428 2803-2815 NNS denotes supernatants
T8427 2795-2802 NNP denotes Culture
T8426 2793-2794 . denotes .
T8425 2792-2793 NNP denotes ]
T8424 2790-2792 CD denotes 28
T8423 2789-2790 NNP denotes [
T8422 2785-2788 CD denotes CO2
T8421 2783-2784 NN denotes %
T8420 2782-2783 CD denotes 5
T8419 2771-2781 VBG denotes containing
T8418 2760-2770 NN denotes atmosphere
T8417 2749-2759 JJ denotes humidified
T8416 2747-2748 DT denotes a
T8415 2744-2746 IN denotes in
T8414 2742-2743 NNP denotes C
T8413 2741-2742 CD denotes °
T8412 2739-2741 CD denotes 37
T8411 2736-2738 IN denotes at
T8410 2734-2735 -RRB- denotes )
T8409 2731-2734 NNP denotes USA
T8408 2729-2730 , denotes ,
T8407 2727-2729 NNP denotes CA
T8406 2725-2726 , denotes ,
T8405 2721-2725 NNP denotes Jose
T8404 2717-2720 NNP denotes San
T8403 2715-2716 , denotes ,
T8402 2706-2715 NNP denotes Dickinson
T8401 2699-2705 NNP denotes Becton
T8400 2698-2699 -LRB- denotes (
T8399 2692-2697 NNP denotes IL-10
T8398 2689-2691 IN denotes of
T8397 2674-2688 NNS denotes concentrations
T8396 2664-2673 VBN denotes indicated
T8395 2660-2663 DT denotes the
T8394 2657-2659 IN denotes of
T8393 2649-2656 NN denotes absence
T8392 2646-2648 CC denotes or
T8391 2637-2645 NN denotes presence
T8390 2633-2636 DT denotes the
T8389 2630-2632 IN denotes in
T8388 2628-2629 -RRB- denotes )
T8387 2618-2628 NNP denotes Immunotech
T8386 2617-2618 -LRB- denotes (
T8385 2613-2616 NNP denotes mAb
T8384 2603-2612 JJ denotes anti-CD28
T8383 2600-2602 NN denotes ml
T8382 2599-2600 NN denotes /
T8381 2597-2599 NN denotes μg
T8380 2595-2596 CD denotes 1
T8379 2590-2594 IN denotes with
T8378 2580-2589 VBN denotes incubated
T8377 2575-2579 VBD denotes were
T8376 2569-2574 NNS denotes cells
T8375 2565-2568 DT denotes The
T8374 2563-2564 . denotes .
T8373 2562-2563 -RRB- denotes )
T8372 2556-2562 NNP denotes France
T8371 2554-2555 , denotes ,
T8370 2545-2554 NNP denotes Marseille
T8369 2543-2544 , denotes ,
T8368 2533-2543 NNP denotes Immunotech
T8367 2532-2533 -LRB- denotes (
T8366 2528-2531 NNP denotes mAb
T8365 2519-2527 JJ denotes anti-CD3
T8364 2516-2518 NN denotes ml
T8363 2515-2516 NN denotes /
T8362 2513-2515 NN denotes μg
T8361 2511-2512 CD denotes 1
T8360 2506-2510 IN denotes with
T8359 2499-2505 VBN denotes coated
T8358 2497-2498 -RRB- denotes )
T8357 2491-2497 NNP denotes Coster
T8356 2490-2491 -LRB- denotes (
T8355 2483-2489 NNS denotes plates
T8354 2472-2482 NN denotes microtiter
T8353 2464-2471 JJ denotes 24-well
T8352 2461-2463 IN denotes of
T8351 2455-2460 NNS denotes wells
T8350 2451-2454 DT denotes the
T8349 2446-2450 IN denotes into
T8348 2436-2445 VBN denotes dispensed
T8347 2431-2435 VBD denotes were
T8346 2419-2430 NNS denotes suspensions
T8345 2414-2418 NN denotes cell
T8344 2411-2413 NN denotes ml
T8343 2407-2410 CD denotes 0.5
T8342 2403-2406 CC denotes and
T8341 2401-2402 , denotes ,
T8340 2398-2401 NNP denotes FCS
T8339 2396-2397 NN denotes %
T8338 2394-2396 CD denotes 10
T8337 2389-2393 IN denotes with
T8336 2382-2388 NN denotes medium
T8335 2374-2381 NN denotes culture
T8334 2371-2373 IN denotes in
T8333 2362-2370 NN denotes cells/ml
T8332 2358-2361 CD denotes 106
T8331 2356-2357 CD denotes ×
T8330 2354-2355 CD denotes 1
T8329 2351-2353 IN denotes of
T8328 2343-2350 NN denotes density
T8327 2341-2342 DT denotes a
T8326 2338-2340 IN denotes at
T8325 2326-2337 VBN denotes resuspended
T8324 2321-2325 VBD denotes were
T8323 2309-2320 NNS denotes populations
T8322 2302-2308 NNP denotes T-cell
T8321 2300-2301 NNP denotes +
T8320 2297-2300 NNP denotes CD4
T8319 2294-2296 NNP denotes PB
T8318 2288-2293 NNS denotes cells
T8317 2286-2287 NNP denotes T
T8316 2284-2285 NNP denotes +
T8315 2281-2284 NNP denotes CD4
T8314 2278-2280 IN denotes of
T8313 2270-2277 NNP denotes Culture
T7876 2097-2098 CD denotes °
T7875 2095-2097 CD denotes 37
T7874 2092-2094 IN denotes at
T7873 2082-2091 VBN denotes incubated
T7872 2077-2081 VBD denotes were
T7871 2075-2076 -RRB- denotes )
T7870 2063-2075 NNPS denotes Technologies
T7869 2058-2062 NNP denotes Life
T7868 2057-2058 -LRB- denotes (
T7867 2053-2056 NNP denotes FCS
T7866 2036-2052 JJ denotes heat-inactivated
T7865 2034-2035 NN denotes %
T7864 2032-2034 CD denotes 10
T7863 2027-2031 IN denotes with
T7862 2025-2026 -RRB- denotes )
T7861 2013-2025 NNPS denotes Technologies
T7860 2008-2012 NNP denotes Life
T7859 2006-2007 : denotes ;
T7858 1994-2006 NN denotes streptomycin
T7857 1988-1993 JJ denotes mg/ml
T7856 1984-1987 CD denotes 100
T7855 1980-1983 CC denotes and
T7854 1978-1979 , denotes ,
T7853 1968-1978 NN denotes penicillin
T7852 1962-1967 NNP denotes IU/ml
T7851 1958-1961 CD denotes 100
T7850 1956-1957 , denotes ,
T7849 1951-1956 NNS denotes acids
T7848 1945-1950 JJ denotes amino
T7847 1932-1944 JJ denotes nonessential
T7846 1930-1931 NN denotes %
T7845 1929-1930 CD denotes 2
T7840 1910-1911 -LRB- denotes (
T7839 1904-1909 NNP denotes HEPES
T7838 1901-1903 NNP denotes mM
T7837 1898-1900 CD denotes 25
T7836 1893-1897 IN denotes with
T7835 1880-1892 VBN denotes supplemented
T7834 1878-1879 -RRB- denotes )
T7833 1875-1878 NNP denotes USA
T7832 1873-1874 , denotes ,
T7831 1871-1873 NNP denotes MD
T7830 1869-1870 , denotes ,
T7829 1857-1869 NNP denotes Gaithersburg
T7828 1855-1856 , denotes ,
T7827 1843-1855 NNPS denotes Technologies
T7826 1838-1842 NNP denotes Life
T7825 1836-1837 : denotes ;
T7824 1830-1836 NN denotes medium
T7823 1825-1829 CD denotes 1640
T7822 1820-1824 NNP denotes RPMI
T7821 1819-1820 -LRB- denotes (
T7820 1812-1818 NN denotes medium
T7819 1804-1811 NN denotes culture
T7818 1801-1803 IN denotes in
T7817 1789-1800 NNS denotes suspensions
T7816 1784-1788 NN denotes cell
T7815 1781-1783 NNP denotes ST
T7814 1779-1780 , denotes ,
T7813 1773-1779 NN denotes debris
T7812 1766-1772 NN denotes tissue
T7811 1757-1765 VBG denotes removing
T7810 1751-1756 IN denotes After
T7809 1749-1750 . denotes .
T7808 1748-1749 NNP denotes C
T7807 1747-1748 CD denotes °
T7806 1745-1747 CD denotes 37
T7805 1742-1744 IN denotes at
T7804 1737-1741 NN denotes hour
T7803 1735-1736 CD denotes 1
T7802 1731-1734 IN denotes for
T7801 1725-1730 NNP denotes DNase
T7800 1721-1724 CC denotes and
T7799 1709-1720 NN denotes collagenase
T7798 1704-1708 IN denotes with
T7797 1695-1703 VBN denotes digested
T7796 1691-1694 CC denotes and
T7795 1680-1690 VBN denotes fragmented
T7794 1675-1679 VBD denotes were
T7793 1667-1674 NNS denotes samples
T7792 1664-1666 NNP denotes ST
T7791 1658-1663 JJ denotes fresh
T7790 1656-1657 , denotes ,
T7789 1649-1656 RB denotes Briefly
T7788 1647-1648 . denotes .
T7787 1646-1647 NNP denotes ]
T7786 1644-1646 CD denotes 27
T7785 1643-1644 NNP denotes [
T7784 1633-1642 VBN denotes described
T7783 1622-1632 RB denotes previously
T7782 1619-1621 IN denotes as
T7781 1617-1618 , denotes ,
T7780 1610-1617 NNS denotes samples
T7779 1607-1609 NNP denotes ST
T7778 1602-1606 IN denotes from
T7777 1593-1601 VBN denotes isolated
T7776 1588-1592 VBD denotes were
T7775 1582-1587 NNS denotes cells
T7774 1580-1581 NNP denotes T
T7773 1578-1579 CD denotes +
T7772 1575-1578 CD denotes CD4
T7771 1573-1574 . denotes .
T7770 1561-1573 NNS denotes instructions
T7769 1558-1560 POS denotes 's
T7768 1546-1558 NN denotes manufacturer
T7767 1542-1545 DT denotes the
T7766 1539-1541 TO denotes to
T7765 1529-1538 VBG denotes according
T7764 1527-1528 , denotes ,
T7763 1526-1527 -RRB- denotes )
T7762 1519-1526 NNP denotes Germany
T7761 1517-1518 , denotes ,
T7760 1509-1517 NNP denotes Gladbach
T7759 1507-1508 , denotes ,
T7758 1501-1507 NNP denotes Biotec
T7757 1492-1500 NNP denotes Miltenyi
T7756 1491-1492 -LRB- denotes (
T7755 1485-1490 NNS denotes beads
T7754 1476-1484 JJ denotes magnetic
T7753 1465-1475 JJ denotes mAb-coated
T7752 1456-1464 JJ denotes anti-CD4
T7751 1450-1455 VBG denotes using
T7750 1440-1449 NN denotes selection
T7749 1431-1439 JJ denotes positive
T7748 1428-1430 IN denotes by
T7747 1423-1427 NNP denotes PBMC
T7746 1418-1422 IN denotes from
T7745 1409-1417 VBN denotes purified
T7744 1404-1408 VBD denotes were
T7743 1398-1403 NNS denotes cells
T7742 1396-1397 NNP denotes T
T7741 1394-1395 CD denotes +
T7740 1391-1394 CD denotes CD4
T7739 1389-1390 . denotes .
T7738 1388-1389 -RRB- denotes )
T7737 1382-1388 NNP denotes Sweden
T7736 1380-1381 , denotes ,
T7735 1373-1380 NNP denotes Uppsala
T7734 1371-1372 , denotes ,
T7733 1362-1371 NNP denotes Pharmacia
T7732 1361-1362 -LRB- denotes (
T7731 1351-1360 NNS denotes gradients
T7730 1343-1350 NN denotes density
T7729 1328-1342 JJ denotes Ficoll-Hypaque
T7728 1323-1327 IN denotes over
T7727 1308-1322 NN denotes centrifugation
T7726 1305-1307 IN denotes by
T7725 1297-1304 NNS denotes samples
T7724 1291-1296 NN denotes blood
T7723 1279-1290 VBN denotes heparinized
T7722 1274-1278 IN denotes from
T7721 1265-1273 VBN denotes prepared
T7720 1260-1264 VBD denotes were
T7719 1258-1259 -RRB- denotes )
T7718 1254-1258 NNP denotes PBMC
T7717 1253-1254 -LRB- denotes (
T7716 1247-1252 NNS denotes cells
T7715 1235-1246 NN denotes mononuclear
T7714 1229-1234 NN denotes blood
T7713 1218-1228 JJ denotes Peripheral
T7712 1212-1217 NNS denotes cells
T7711 1210-1211 NNP denotes T
T7710 1208-1209 CD denotes +
T7709 1205-1208 CD denotes CD4
T7708 1202-1204 IN denotes of
T7707 1192-1201 NN denotes Isolation
T7844 1927-1928 , denotes ,
T7843 1916-1927 NNP denotes L-glutamine
T7842 1913-1915 NNP denotes mM
T7841 1911-1912 CD denotes 2
T7232 832-833 : denotes ;
T7231 831-832 -RRB- denotes )
T7230 827-831 NN denotes mg/l
T7229 824-826 CD denotes 10
T7228 822-823 FW denotes ≥
T7227 821-822 -LRB- denotes (
T7226 815-820 NN denotes level
T7225 811-814 NNP denotes CRP
T7224 805-810 NN denotes serum
T7223 796-804 JJ denotes elevated
T7222 792-795 CC denotes and
T7221 785-791 NNS denotes joints
T7220 777-784 JJ denotes swollen
T7219 770-776 CC denotes and/or
T7218 763-769 NN denotes tender
T7217 754-762 JJ denotes multiple
T7216 750-753 VBD denotes had
T7215 746-749 WP denotes who
T7214 744-745 , denotes ,
T7213 737-744 NN denotes disease
T7212 730-736 JJ denotes active
T7211 725-729 IN denotes with
T7210 716-724 NNS denotes patients
T7209 713-715 CD denotes 24
T7208 711-712 : denotes :
T7207 705-711 NNS denotes groups
T7206 701-704 CD denotes two
T7205 696-700 IN denotes into
T7204 688-695 VBN denotes divided
T7203 683-687 VBD denotes were
T7202 674-682 NNS denotes Patients
T7201 672-673 . denotes .
T7200 668-672 NNP denotes U/ml
T7199 664-667 CD denotes 158
T7198 662-663 NN denotes ±
T7197 658-661 CD denotes 142
T7196 656-657 , denotes ,
T7195 651-656 NN denotes titer
T7194 644-650 NN denotes factor
T7193 633-643 JJ denotes rheumatoid
T7192 627-632 NN denotes class
T7191 623-626 NNP denotes IgM
T7190 619-622 CC denotes and
T7189 617-618 : denotes ;
T7188 613-617 NN denotes mg/l
T7187 608-612 CD denotes 32.0
T7186 606-607 CD denotes ±
T7185 601-605 CD denotes 32.0
T7184 599-600 , denotes ,
T7183 594-599 NN denotes level
T7182 592-593 -RRB- denotes )
T7181 589-592 NNP denotes CRP
T7180 588-589 -LRB- denotes (
T7179 580-587 NN denotes protein
T7178 569-579 JJ denotes C-reactive
T7177 563-568 NN denotes serum
T7176 561-562 : denotes ;
T7175 554-561 NN denotes mm/hour
T7174 549-553 CD denotes 35.4
T7173 547-548 CD denotes ±
T7172 542-546 CD denotes 55.9
T7171 540-541 , denotes ,
T7170 536-540 NN denotes rate
T7169 522-535 NN denotes sedimentation
T7168 510-521 VB denotes erythrocyte
T7167 508-509 : denotes :
T7166 507-508 -RRB- denotes )
T7165 498-507 NN denotes deviation
T7164 489-497 JJ denotes standard
T7163 487-488 JJ denotes ±
T7162 482-486 VB denotes mean
T7161 481-482 -LRB- denotes (
T7160 473-480 VBZ denotes follows
T7159 470-472 IN denotes as
T7158 465-469 VBD denotes were
T7157 456-464 NNS denotes patients
T7156 450-455 NN denotes study
T7155 446-449 DT denotes the
T7154 443-445 IN denotes in
T7153 432-442 NNS denotes parameters
T7152 423-431 JJ denotes Clinical
T7151 421-422 . denotes .
T7150 416-421 NNS denotes drugs
T7149 402-415 JJ denotes antirheumatic
T7148 384-401 JJ denotes disease-modifying
T7147 380-383 CC denotes and
T7146 378-379 -RRB- denotes )
T7145 372-378 NN denotes mg/day
T7144 368-371 CD denotes 7.5
T7143 366-367 CD denotes ≤
T7142 365-366 -LRB- denotes (
T7141 352-364 NN denotes prednisolone
T7140 342-351 VBG denotes receiving
T7139 337-341 VBD denotes were
T7138 328-336 NNS denotes patients
T7137 324-327 DT denotes All
T7136 322-323 . denotes .
T7135 321-322 NNP denotes ]
T7134 319-321 CD denotes 25
T7133 318-319 NNP denotes [
T7132 316-317 -RRB- denotes )
T7131 305-316 NNP denotes Association
T7130 294-304 NNP denotes Rheumatism
T7129 285-293 JJ denotes American
T7128 281-284 DT denotes the
T7127 279-280 , denotes ,
T7126 271-279 RB denotes formally
T7125 270-271 -LRB- denotes (
T7124 257-269 NNP denotes Rheumatology
T7123 254-256 IN denotes of
T7122 246-253 NNP denotes College
T7121 237-245 JJ denotes American
T7120 233-236 DT denotes the
T7119 230-232 IN denotes of
T7118 221-229 NNS denotes criteria
T7117 216-220 CD denotes 1987
T7116 208-215 VBN denotes revised
T7115 204-207 DT denotes the
T7114 201-203 TO denotes to
T7113 191-200 VBG denotes according
T7112 181-190 VBN denotes diagnosed
T7111 179-180 -RRB- denotes )
T7110 174-179 NNS denotes years
T7109 169-173 CD denotes 12.4
T7108 167-168 NN denotes ±
T7107 162-166 CD denotes 52.8
T7106 160-161 , denotes ,
T7105 157-160 NN denotes age
T7104 147-156 NN denotes deviation
T7103 138-146 JJ denotes standard
T7102 136-137 JJ denotes ±
T7101 131-135 VB denotes mean
T7100 129-130 : denotes ;
T7099 126-129 NNS denotes men
T7098 120-125 CD denotes seven
T7097 116-119 CC denotes and
T7096 110-115 NNS denotes women
T7095 107-109 CD denotes 25
T7094 106-107 -LRB- denotes (
T7093 103-105 NNP denotes RA
T7092 98-102 IN denotes with
T7091 89-97 NNS denotes patients
T7090 86-88 CD denotes 32
T7089 83-85 IN denotes of
T7088 73-82 VBD denotes consisted
T7087 62-72 NN denotes population
T7086 54-61 JJ denotes patient
T7085 48-53 JJ denotes total
T7084 44-47 DT denotes The
T7083 36-43 NNS denotes samples
T7082 32-35 CC denotes and
T7081 23-31 NNS denotes Patients
T7295 1189-1190 . denotes .
T7294 1182-1189 NN denotes consent
T7293 1173-1181 VBN denotes informed
T7292 1168-1172 VBD denotes gave
T7291 1159-1167 NNS denotes patients
T7290 1155-1158 DT denotes All
T7289 1153-1154 . denotes .
T7288 1142-1153 NN denotes replacement
T7287 1137-1141 NN denotes knee
T7286 1131-1136 JJ denotes total
T7285 1120-1130 VBG denotes undergoing
T7265 1026-1027 : denotes ;
T7264 1023-1026 NNS denotes men
T7263 1018-1022 CD denotes five
T7262 1014-1017 CC denotes and
T7261 1008-1013 NNS denotes women
T7260 1005-1007 CD denotes 11
T7259 1004-1005 -LRB- denotes (
T7258 993-1003 NNS denotes volunteers
T7257 985-992 JJ denotes healthy
T7256 977-984 CD denotes Sixteen
T7255 975-976 . denotes .
T7254 974-975 NNP denotes ]
T7253 972-974 CD denotes 26
T7252 971-972 NNP denotes [
T7251 961-970 NN denotes remission
T7250 952-960 JJ denotes clinical
T7249 948-951 IN denotes for
T7248 939-947 NNS denotes criteria
T7247 927-938 JJ denotes preliminary
T7246 914-926 NNP denotes Rheumatology
T7245 911-913 IN denotes of
T7244 903-910 NNP denotes College
T7243 894-902 JJ denotes American
T7242 890-893 DT denotes the
T7241 880-889 VBD denotes satisfied
T7240 876-879 WP denotes who
T7239 874-875 , denotes ,
T7238 867-874 NN denotes disease
T7237 858-866 JJ denotes inactive
T7236 853-857 IN denotes with
T7235 844-852 NNS denotes patients
T7234 838-843 CD denotes eight
T7233 834-837 CC denotes and
T7284 1111-1119 NNS denotes patients
T7283 1108-1110 NNP denotes RA
T7282 1102-1107 CD denotes three
T7281 1097-1101 IN denotes from
T7280 1088-1096 VBN denotes obtained
T7279 1083-1087 VBD denotes were
T7278 1075-1082 NNS denotes samples
T7277 1072-1074 NNP denotes ST
T7276 1070-1071 . denotes .
T7275 1062-1070 NNS denotes controls
T7274 1059-1061 IN denotes as
T7273 1052-1058 VBD denotes served
T7272 1050-1051 -RRB- denotes )
T7271 1045-1050 NNS denotes years
T7270 1040-1044 CD denotes 11.2
T7269 1038-1039 NN denotes ±
T7268 1033-1037 CD denotes 45.8
T7267 1031-1032 , denotes ,
T7266 1028-1031 NN denotes age
R3398 T7081 T7081 ROOT Patients,Patients
R3399 T7082 T7081 cc and,Patients
R3400 T7083 T7081 conj samples,Patients
R3401 T7084 T7087 det The,population
R3402 T7085 T7087 amod total,population
R3403 T7086 T7087 amod patient,population
R3404 T7087 T7088 nsubj population,consisted
R3405 T7088 T7101 ccomp consisted,mean
R3406 T7089 T7088 prep of,consisted
R3407 T7090 T7091 nummod 32,patients
R3408 T7091 T7089 pobj patients,of
R3409 T7092 T7091 prep with,patients
R3410 T7093 T7092 pobj RA,with
R3411 T7094 T7093 punct (,RA
R3412 T7095 T7096 nummod 25,women
R3413 T7096 T7093 appos women,RA
R3414 T7097 T7096 cc and,women
R3415 T7098 T7099 nummod seven,men
R3416 T7099 T7096 conj men,women
R3417 T7100 T7101 punct ;,mean
R3418 T7101 T7101 ROOT mean,mean
R3419 T7102 T7105 amod ±,age
R3420 T7103 T7104 amod standard,deviation
R3421 T7104 T7105 compound deviation,age
R3422 T7105 T7101 dobj age,mean
R3423 T7106 T7105 punct ",",age
R3424 T7107 T7108 nummod 52.8,±
R3425 T7108 T7105 appos ±,age
R3426 T7109 T7110 nummod 12.4,years
R3427 T7110 T7108 appos years,±
R3428 T7111 T7105 punct ),age
R3429 T7112 T7105 acl diagnosed,age
R3430 T7113 T7112 prep according,diagnosed
R3431 T7114 T7113 prep to,according
R3432 T7115 T7118 det the,criteria
R3433 T7116 T7118 amod revised,criteria
R3434 T7117 T7118 nummod 1987,criteria
R3435 T7118 T7114 pobj criteria,to
R3436 T7119 T7118 prep of,criteria
R3437 T7120 T7122 det the,College
R3438 T7121 T7122 compound American,College
R3439 T7122 T7119 pobj College,of
R3440 T7123 T7122 prep of,College
R3441 T7124 T7123 pobj Rheumatology,of
R3442 T7125 T7131 punct (,Association
R3443 T7126 T7131 advmod formally,Association
R3444 T7127 T7131 punct ",",Association
R3445 T7128 T7131 det the,Association
R3446 T7129 T7131 compound American,Association
R3447 T7130 T7131 compound Rheumatism,Association
R3448 T7131 T7122 appos Association,College
R3449 T7132 T7133 punct ),[
R3450 T7133 T7135 nmod [,]
R3451 T7134 T7135 nummod 25,]
R3452 T7135 T7131 appos ],Association
R3453 T7136 T7101 punct .,mean
R3454 T7137 T7138 det All,patients
R3455 T7138 T7140 nsubj patients,receiving
R3456 T7139 T7140 aux were,receiving
R3457 T7140 T7140 ROOT receiving,receiving
R3458 T7141 T7140 dobj prednisolone,receiving
R3459 T7142 T7141 punct (,prednisolone
R3460 T7143 T7141 appos ≤,prednisolone
R3461 T7144 T7145 nummod 7.5,mg/day
R3462 T7145 T7143 appos mg/day,≤
R3463 T7146 T7145 punct ),mg/day
R3464 T7147 T7145 cc and,mg/day
R3465 T7148 T7150 amod disease-modifying,drugs
R3466 T7149 T7150 amod antirheumatic,drugs
R3467 T7150 T7145 conj drugs,mg/day
R3468 T7151 T7140 punct .,receiving
R3469 T7152 T7153 amod Clinical,parameters
R3470 T7153 T7158 nsubj parameters,were
R3471 T7154 T7153 prep in,parameters
R3472 T7155 T7157 det the,patients
R3473 T7156 T7157 compound study,patients
R3474 T7157 T7154 pobj patients,in
R3475 T7158 T7158 ROOT were,were
R3476 T7159 T7160 mark as,follows
R3477 T7160 T7158 advcl follows,were
R3478 T7161 T7162 punct (,mean
R3479 T7162 T7170 meta mean,rate
R3480 T7163 T7165 nmod ±,deviation
R3481 T7164 T7165 amod standard,deviation
R3482 T7165 T7162 dobj deviation,mean
R3483 T7166 T7162 punct ),mean
R3484 T7167 T7162 punct :,mean
R3485 T7168 T7170 compound erythrocyte,rate
R3486 T7169 T7170 compound sedimentation,rate
R3487 T7170 T7160 nsubj rate,follows
R3488 T7171 T7170 punct ",",rate
R3489 T7172 T7175 nummod 55.9,mm/hour
R3490 T7173 T7175 nmod ±,mm/hour
R3491 T7174 T7175 nummod 35.4,mm/hour
R3492 T7175 T7170 appos mm/hour,rate
R3493 T7176 T7170 punct ;,rate
R3494 T7177 T7179 nmod serum,protein
R3495 T7178 T7179 amod C-reactive,protein
R3496 T7179 T7170 appos protein,rate
R3497 T7180 T7181 punct (,CRP
R3498 T7181 T7179 appos CRP,protein
R3499 T7182 T7181 punct ),CRP
R3500 T7183 T7179 appos level,protein
R3501 T7184 T7179 punct ",",protein
R3502 T7185 T7188 nummod 32.0,mg/l
R3503 T7186 T7188 nummod ±,mg/l
R3504 T7187 T7188 nummod 32.0,mg/l
R3505 T7188 T7179 appos mg/l,protein
R3506 T7189 T7170 punct ;,rate
R3507 T7190 T7170 cc and,rate
R3508 T7191 T7192 compound IgM,class
R3509 T7192 T7194 compound class,factor
R3510 T7193 T7194 compound rheumatoid,factor
R3511 T7194 T7195 compound factor,titer
R3512 T7195 T7170 conj titer,rate
R3513 T7196 T7195 punct ",",titer
R3514 T7197 T7199 quantmod 142,158
R3515 T7198 T7199 quantmod ±,158
R3516 T7199 T7200 nummod 158,U/ml
R3517 T7200 T7170 conj U/ml,rate
R3518 T7201 T7158 punct .,were
R3519 T7202 T7204 nsubjpass Patients,divided
R3520 T7203 T7204 auxpass were,divided
R3521 T7204 T7204 ROOT divided,divided
R3522 T7205 T7204 prep into,divided
R3523 T7206 T7207 nummod two,groups
R3524 T7207 T7205 pobj groups,into
R3525 T7208 T7207 punct :,groups
R3526 T7209 T7210 nummod 24,patients
R3527 T7210 T7207 appos patients,groups
R3528 T7211 T7210 prep with,patients
R3529 T7212 T7213 amod active,disease
R3530 T7213 T7211 pobj disease,with
R3531 T7214 T7213 punct ",",disease
R3532 T7215 T7216 nsubj who,had
R3533 T7216 T7213 relcl had,disease
R3534 T7217 T7218 amod multiple,tender
R3535 T7218 T7216 dobj tender,had
R3536 T7219 T7218 cc and/or,tender
R3537 T7220 T7221 amod swollen,joints
R3538 T7221 T7218 conj joints,tender
R3539 T7222 T7221 cc and,joints
R3540 T7223 T7224 amod elevated,serum
R3541 T7224 T7221 conj serum,joints
R3542 T7225 T7226 compound CRP,level
R3543 T7226 T7216 npadvmod level,had
R3544 T7227 T7228 punct (,≥
R3545 T7228 T7230 nmod ≥,mg/l
R3546 T7229 T7230 nummod 10,mg/l
R3547 T7230 T7226 appos mg/l,level
R3548 T7231 T7226 punct ),level
R3549 T7232 T7216 punct ;,had
R3550 T7233 T7210 cc and,patients
R3551 T7234 T7235 nummod eight,patients
R3552 T7235 T7210 conj patients,patients
R3553 T7236 T7235 prep with,patients
R3554 T7237 T7238 amod inactive,disease
R3555 T7238 T7236 pobj disease,with
R3556 T7239 T7238 punct ",",disease
R3557 T7240 T7241 nsubj who,satisfied
R3558 T7241 T7210 relcl satisfied,patients
R3559 T7242 T7244 det the,College
R3560 T7243 T7244 compound American,College
R3561 T7244 T7241 dobj College,satisfied
R3562 T7245 T7244 prep of,College
R3563 T7246 T7248 amod Rheumatology,criteria
R3564 T7247 T7248 amod preliminary,criteria
R3565 T7248 T7245 pobj criteria,of
R3566 T7249 T7248 prep for,criteria
R3567 T7250 T7251 amod clinical,remission
R3568 T7251 T7249 pobj remission,for
R3569 T7252 T7254 nmod [,]
R3570 T7253 T7254 nummod 26,]
R3571 T7254 T7244 appos ],College
R3572 T7255 T7204 punct .,divided
R3573 T7256 T7258 nummod Sixteen,volunteers
R3574 T7257 T7258 amod healthy,volunteers
R3575 T7258 T7273 nsubj volunteers,served
R3576 T7259 T7258 punct (,volunteers
R3577 T7260 T7261 nummod 11,women
R3578 T7261 T7258 appos women,volunteers
R3579 T7262 T7261 cc and,women
R3580 T7263 T7264 nummod five,men
R3581 T7264 T7261 conj men,women
R3582 T7265 T7258 punct ;,volunteers
R3583 T7266 T7258 appos age,volunteers
R3584 T7267 T7266 punct ",",age
R3585 T7268 T7269 nummod 45.8,±
R3586 T7269 T7266 appos ±,age
R3590 T7273 T7273 ROOT served,served
R3591 T7274 T7273 prep as,served
R3592 T7275 T7274 pobj controls,as
R3593 T7276 T7273 punct .,served
R3594 T7277 T7278 compound ST,samples
R3595 T7278 T7280 nsubjpass samples,obtained
R3596 T7279 T7280 auxpass were,obtained
R3597 T7280 T7280 ROOT obtained,obtained
R3598 T7281 T7280 prep from,obtained
R3599 T7282 T7284 nummod three,patients
R3600 T7283 T7284 compound RA,patients
R3601 T7284 T7281 pobj patients,from
R3602 T7285 T7284 acl undergoing,patients
R3603 T7286 T7288 amod total,replacement
R3604 T7287 T7288 compound knee,replacement
R3605 T7288 T7285 dobj replacement,undergoing
R3606 T7289 T7280 punct .,obtained
R3607 T7290 T7291 det All,patients
R3608 T7291 T7292 nsubj patients,gave
R3609 T7292 T7292 ROOT gave,gave
R3610 T7293 T7294 amod informed,consent
R3611 T7294 T7292 dobj consent,gave
R3612 T7295 T7292 punct .,gave
R3808 T7707 T7721 nsubjpass Isolation,prepared
R3809 T7708 T7707 prep of,Isolation
R3810 T7709 T7712 nummod CD4,cells
R3811 T7710 T7712 nummod +,cells
R3812 T7711 T7712 compound T,cells
R3813 T7712 T7708 pobj cells,of
R3814 T7713 T7716 amod Peripheral,cells
R3815 T7714 T7716 compound blood,cells
R3816 T7715 T7716 compound mononuclear,cells
R3817 T7716 T7712 appos cells,cells
R3818 T7717 T7718 punct (,PBMC
R3819 T7718 T7716 appos PBMC,cells
R3820 T7719 T7716 punct ),cells
R3821 T7720 T7721 auxpass were,prepared
R3822 T7721 T7721 ROOT prepared,prepared
R3823 T7722 T7721 prep from,prepared
R3824 T7723 T7725 amod heparinized,samples
R3825 T7724 T7725 compound blood,samples
R3826 T7725 T7722 pobj samples,from
R3827 T7726 T7721 agent by,prepared
R3828 T7727 T7726 pobj centrifugation,by
R3829 T7728 T7727 prep over,centrifugation
R3830 T7729 T7730 amod Ficoll-Hypaque,density
R3831 T7730 T7731 compound density,gradients
R3832 T7731 T7728 pobj gradients,over
R3833 T7732 T7738 punct (,)
R3834 T7733 T7738 nmod Pharmacia,)
R3835 T7734 T7733 punct ",",Pharmacia
R3836 T7735 T7733 conj Uppsala,Pharmacia
R3837 T7736 T7735 punct ",",Uppsala
R3838 T7737 T7735 conj Sweden,Uppsala
R3839 T7738 T7727 punct ),centrifugation
R3840 T7739 T7721 punct .,prepared
R3841 T7740 T7743 nummod CD4,cells
R3842 T7741 T7743 nummod +,cells
R3843 T7742 T7743 compound T,cells
R3844 T7743 T7745 nsubjpass cells,purified
R3845 T7744 T7745 auxpass were,purified
R3846 T7745 T7745 ROOT purified,purified
R3847 T7746 T7745 prep from,purified
R3848 T7747 T7746 pobj PBMC,from
R3849 T7748 T7745 agent by,purified
R3850 T7749 T7750 amod positive,selection
R3851 T7750 T7748 pobj selection,by
R3852 T7751 T7745 advcl using,purified
R3853 T7752 T7755 amod anti-CD4,beads
R3854 T7753 T7755 amod mAb-coated,beads
R3855 T7754 T7755 amod magnetic,beads
R3856 T7755 T7751 dobj beads,using
R3857 T7756 T7755 punct (,beads
R3858 T7757 T7758 compound Miltenyi,Biotec
R3859 T7758 T7755 appos Biotec,beads
R3860 T7759 T7758 punct ",",Biotec
R3861 T7760 T7758 conj Gladbach,Biotec
R3862 T7761 T7760 punct ",",Gladbach
R3863 T7762 T7760 conj Germany,Gladbach
R3864 T7763 T7755 punct ),beads
R3865 T7764 T7745 punct ",",purified
R3866 T7765 T7745 prep according,purified
R3867 T7766 T7765 prep to,according
R3868 T7767 T7768 det the,manufacturer
R3869 T7768 T7770 poss manufacturer,instructions
R3870 T7769 T7768 case 's,manufacturer
R3871 T7770 T7766 pobj instructions,to
R3872 T7771 T7745 punct .,purified
R3873 T7772 T7775 nummod CD4,cells
R3874 T7773 T7775 nummod +,cells
R3875 T7774 T7775 compound T,cells
R3876 T7775 T7777 nsubjpass cells,isolated
R3877 T7776 T7777 auxpass were,isolated
R3878 T7777 T7777 ROOT isolated,isolated
R3879 T7778 T7777 prep from,isolated
R3880 T7779 T7780 compound ST,samples
R3881 T7780 T7778 pobj samples,from
R3882 T7781 T7777 punct ",",isolated
R3883 T7782 T7784 mark as,described
R3884 T7783 T7784 advmod previously,described
R3885 T7784 T7777 advcl described,isolated
R3886 T7785 T7787 nmod [,]
R3887 T7786 T7787 nummod 27,]
R3888 T7787 T7784 dobj ],described
R3889 T7788 T7777 punct .,isolated
R3890 T7789 T7795 advmod Briefly,fragmented
R3891 T7790 T7795 punct ",",fragmented
R3892 T7791 T7793 amod fresh,samples
R3893 T7792 T7793 compound ST,samples
R3894 T7793 T7795 nsubjpass samples,fragmented
R3895 T7794 T7795 auxpass were,fragmented
R3896 T7795 T7795 ROOT fragmented,fragmented
R3897 T7796 T7795 cc and,fragmented
R3898 T7797 T7795 conj digested,fragmented
R3899 T7798 T7797 prep with,digested
R3900 T7799 T7798 pobj collagenase,with
R3901 T7800 T7799 cc and,collagenase
R3902 T7801 T7799 conj DNase,collagenase
R3903 T7802 T7797 prep for,digested
R3904 T7803 T7804 nummod 1,hour
R3905 T7804 T7802 pobj hour,for
R3906 T7805 T7797 prep at,digested
R3907 T7806 T7807 nummod 37,°
R3908 T7807 T7805 pobj °,at
R3909 T7808 T7805 pobj C,at
R3910 T7809 T7795 punct .,fragmented
R3911 T7810 T7817 prep After,suspensions
R3912 T7811 T7810 pcomp removing,After
R3913 T7812 T7813 compound tissue,debris
R3914 T7813 T7811 dobj debris,removing
R3915 T7814 T7817 punct ",",suspensions
R3916 T7815 T7817 compound ST,suspensions
R3917 T7816 T7817 compound cell,suspensions
R3918 T7817 T7835 ccomp suspensions,supplemented
R3919 T7818 T7817 prep in,suspensions
R3920 T7819 T7820 compound culture,medium
R3921 T7820 T7818 pobj medium,in
R3922 T7821 T7824 punct (,medium
R3923 T7822 T7824 nmod RPMI,medium
R3924 T7823 T7822 nummod 1640,RPMI
R3925 T7824 T7817 appos medium,suspensions
R3926 T7825 T7835 punct ;,supplemented
R3927 T7826 T7827 compound Life,Technologies
R3928 T7827 T7835 nsubj Technologies,supplemented
R3929 T7828 T7827 punct ",",Technologies
R3930 T7829 T7827 conj Gaithersburg,Technologies
R3931 T7830 T7829 punct ",",Gaithersburg
R3932 T7831 T7829 conj MD,Gaithersburg
R3933 T7832 T7831 punct ",",MD
R3934 T7833 T7831 conj USA,MD
R3935 T7834 T7827 punct ),Technologies
R3936 T7835 T7835 ROOT supplemented,supplemented
R3937 T7836 T7835 prep with,supplemented
R3938 T7837 T7839 nummod 25,HEPES
R3939 T7838 T7839 compound mM,HEPES
R3940 T7839 T7849 nmod HEPES,acids
R3941 T7840 T7843 punct (,L-glutamine
R3942 T7841 T7843 nummod 2,L-glutamine
R3943 T7842 T7843 compound mM,L-glutamine
R3944 T7843 T7839 appos L-glutamine,HEPES
R3945 T7844 T7843 punct ",",L-glutamine
R3946 T7845 T7846 nummod 2,%
R3947 T7846 T7849 nmod %,acids
R3948 T7847 T7849 amod nonessential,acids
R3949 T7848 T7849 amod amino,acids
R3950 T7849 T7836 pobj acids,with
R3951 T7850 T7849 punct ",",acids
R3952 T7851 T7853 nummod 100,penicillin
R3953 T7852 T7853 compound IU/ml,penicillin
R3954 T7853 T7849 conj penicillin,acids
R3955 T7854 T7853 punct ",",penicillin
R3956 T7855 T7853 cc and,penicillin
R3957 T7856 T7858 nummod 100,streptomycin
R3958 T7857 T7858 amod mg/ml,streptomycin
R3959 T7858 T7853 conj streptomycin,penicillin
R3960 T7859 T7861 punct ;,Technologies
R3961 T7860 T7861 compound Life,Technologies
R3962 T7861 T7835 npadvmod Technologies,supplemented
R3963 T7862 T7861 punct ),Technologies
R3964 T7863 T7861 prep with,Technologies
R3965 T7864 T7865 nummod 10,%
R3966 T7865 T7863 pobj %,with
R3967 T7866 T7867 amod heat-inactivated,FCS
R3968 T7867 T7873 nsubjpass FCS,incubated
R3969 T7868 T7870 punct (,Technologies
R3970 T7869 T7870 compound Life,Technologies
R3971 T7870 T7867 appos Technologies,FCS
R3972 T7871 T7870 punct ),Technologies
R3973 T7872 T7873 auxpass were,incubated
R3974 T7873 T7873 ROOT incubated,incubated
R3975 T7874 T7873 prep at,incubated
R3976 T7875 T7876 nummod 37,°
R3977 T7876 T7877 nummod °,C
R3978 T7877 T7874 pobj C,at
R3979 T7878 T7877 prep in,C
R3980 T7879 T7880 amod six-well,plates
R3981 T7880 T7878 pobj plates,in
R3982 T7881 T7877 punct (,C
R3983 T7882 T7877 appos Coster,C
R3984 T7883 T7882 punct ",",Coster
R3985 T7884 T7882 npadvmod Cambridge,Coster
R3986 T7885 T7884 punct ",",Cambridge
R3987 T7886 T7884 conj MA,Cambridge
R3988 T7887 T7886 punct ",",MA
R3989 T7888 T7886 npadvmod USA,MA
R3990 T7889 T7886 punct ),MA
R3991 T7890 T7884 prep for,Cambridge
R3992 T7891 T7892 nummod 45,min
R3993 T7892 T7890 pobj min,for
R3994 T7893 T7882 punct .,Coster
R3995 T7894 T7895 amod Non-adherent,cells
R3996 T7895 T7897 nsubjpass cells,harvested
R3997 T7896 T7897 auxpass were,harvested
R3998 T7897 T7897 ROOT harvested,harvested
R3999 T7898 T7897 cc and,harvested
R4000 T7899 T7902 compound CD4,cells
R4001 T7900 T7902 nummod +,cells
R4002 T7901 T7902 compound T,cells
R4003 T7902 T7904 nsubjpass cells,purified
R4004 T7903 T7904 auxpass were,purified
R4005 T7904 T7897 conj purified,harvested
R4006 T7905 T7904 agent by,purified
R4007 T7906 T7907 amod positive,selection
R4008 T7907 T7905 pobj selection,by
R4009 T7908 T7910 mark as,described
R4010 T7909 T7910 advmod already,described
R4011 T7910 T7904 advcl described,purified
R4012 T7911 T7904 punct .,purified
R4212 T8313 T8318 nmod Culture,cells
R4213 T8314 T8313 prep of,Culture
R4214 T8315 T8314 pobj CD4,of
R4215 T8316 T8313 conj +,Culture
R4216 T8317 T8318 compound T,cells
R4217 T8318 T8325 nsubjpass cells,resuspended
R4218 T8319 T8323 compound PB,populations
R4219 T8320 T8323 compound CD4,populations
R4220 T8321 T8323 compound +,populations
R4221 T8322 T8323 compound T-cell,populations
R4222 T8323 T8325 nsubjpass populations,resuspended
R4223 T8324 T8325 auxpass were,resuspended
R4224 T8325 T8325 ROOT resuspended,resuspended
R4225 T8326 T8325 prep at,resuspended
R4226 T8327 T8328 det a,density
R4227 T8328 T8326 pobj density,at
R4228 T8329 T8328 prep of,density
R4229 T8330 T8333 nummod 1,cells/ml
R4230 T8331 T8333 nummod ×,cells/ml
R4231 T8332 T8333 nummod 106,cells/ml
R4232 T8333 T8329 pobj cells/ml,of
R4233 T8334 T8333 prep in,cells/ml
R4234 T8335 T8336 compound culture,medium
R4235 T8336 T8334 pobj medium,in
R4236 T8337 T8325 prep with,resuspended
R4237 T8338 T8339 nummod 10,%
R4238 T8339 T8340 compound %,FCS
R4239 T8340 T8337 pobj FCS,with
R4240 T8341 T8325 punct ",",resuspended
R4241 T8342 T8325 cc and,resuspended
R4242 T8343 T8346 nummod 0.5,suspensions
R4243 T8344 T8346 compound ml,suspensions
R4244 T8345 T8346 compound cell,suspensions
R4245 T8346 T8348 nsubjpass suspensions,dispensed
R4246 T8347 T8348 auxpass were,dispensed
R4247 T8348 T8325 conj dispensed,resuspended
R4248 T8349 T8348 prep into,dispensed
R4249 T8350 T8351 det the,wells
R4250 T8351 T8349 pobj wells,into
R4251 T8352 T8351 prep of,wells
R4252 T8353 T8355 amod 24-well,plates
R4253 T8354 T8355 compound microtiter,plates
R4254 T8355 T8352 pobj plates,of
R4255 T8356 T8357 punct (,Coster
R4256 T8357 T8351 appos Coster,wells
R4257 T8358 T8351 punct ),wells
R4258 T8359 T8348 conj coated,dispensed
R4259 T8360 T8359 prep with,coated
R4260 T8361 T8362 nummod 1,μg
R4261 T8362 T8360 pobj μg,with
R4262 T8363 T8364 compound /,ml
R4263 T8364 T8366 compound ml,mAb
R4264 T8365 T8366 compound anti-CD3,mAb
R4265 T8366 T8362 appos mAb,μg
R4266 T8367 T8368 punct (,Immunotech
R4267 T8368 T8366 appos Immunotech,mAb
R4268 T8369 T8368 punct ",",Immunotech
R4269 T8370 T8368 npadvmod Marseille,Immunotech
R4270 T8371 T8370 punct ",",Marseille
R4271 T8372 T8370 conj France,Marseille
R4272 T8373 T8368 punct ),Immunotech
R4273 T8374 T8348 punct .,dispensed
R4274 T8375 T8376 det The,cells
R4275 T8376 T8378 nsubjpass cells,incubated
R4276 T8377 T8378 auxpass were,incubated
R4277 T8378 T8378 ROOT incubated,incubated
R4278 T8379 T8378 prep with,incubated
R4279 T8380 T8381 nummod 1,μg
R4280 T8381 T8385 compound μg,mAb
R4281 T8382 T8383 compound /,ml
R4282 T8383 T8385 compound ml,mAb
R4283 T8384 T8385 compound anti-CD28,mAb
R4284 T8385 T8379 pobj mAb,with
R4285 T8386 T8385 punct (,mAb
R4286 T8387 T8385 appos Immunotech,mAb
R4287 T8388 T8385 punct ),mAb
R4288 T8389 T8378 prep in,incubated
R4289 T8390 T8391 det the,presence
R4290 T8391 T8389 pobj presence,in
R4291 T8392 T8391 cc or,presence
R4292 T8393 T8391 conj absence,presence
R4293 T8394 T8393 prep of,absence
R4294 T8395 T8397 det the,concentrations
R4295 T8396 T8397 amod indicated,concentrations
R4296 T8397 T8394 pobj concentrations,of
R4297 T8398 T8397 prep of,concentrations
R4298 T8399 T8398 pobj IL-10,of
R4299 T8400 T8399 punct (,IL-10
R4300 T8401 T8402 compound Becton,Dickinson
R4301 T8402 T8399 appos Dickinson,IL-10
R4302 T8403 T8402 punct ",",Dickinson
R4303 T8404 T8405 compound San,Jose
R4304 T8405 T8402 npadvmod Jose,Dickinson
R4305 T8406 T8405 punct ",",Jose
R4306 T8407 T8405 conj CA,Jose
R4307 T8408 T8407 punct ",",CA
R4308 T8409 T8399 appos USA,IL-10
R4309 T8410 T8399 punct ),IL-10
R4310 T8411 T8397 prep at,concentrations
R4311 T8412 T8413 nummod 37,°
R4312 T8413 T8414 nummod °,C
R4313 T8414 T8411 pobj C,at
R4314 T8415 T8397 prep in,concentrations
R4315 T8416 T8418 det a,atmosphere
R4316 T8417 T8418 amod humidified,atmosphere
R4317 T8418 T8415 pobj atmosphere,in
R4318 T8419 T8418 acl containing,atmosphere
R4319 T8420 T8421 nummod 5,%
R4320 T8421 T8419 dobj %,containing
R4321 T8422 T8425 nummod CO2,]
R4322 T8423 T8425 nmod [,]
R4323 T8424 T8425 nummod 28,]
R4324 T8425 T8418 appos ],atmosphere
R4325 T8426 T8378 punct .,incubated
R4326 T8427 T8428 compound Culture,supernatants
R4327 T8428 T8430 nsubjpass supernatants,collected
R4328 T8429 T8430 auxpass were,collected
R4329 T8430 T8430 ROOT collected,collected
R4330 T8431 T8432 nummod 36,hours
R4331 T8432 T8433 npadvmod hours,later
R4332 T8433 T8430 advmod later,collected
R4333 T8434 T8433 cc and,later
R4334 T8435 T8436 amod cell-free,samples
R4335 T8436 T8438 nsubjpass samples,stored
R4336 T8437 T8438 auxpass were,stored
R4337 T8438 T8430 conj stored,collected
R4338 T8439 T8438 prep at,stored
R4339 T8440 T8441 nummod -30,°
R4340 T8441 T8439 pobj °,at
R4341 T8442 T8438 conj C,stored
R4342 T8443 T8442 prep until,C
R4343 T8444 T8445 compound cytokine,assay
R4344 T8445 T8443 pobj assay,until
R4345 T8446 T8438 punct .,stored
R4346 T8447 T8448 aux To,examine
R4347 T8448 T8467 advcl examine,incubated
R4348 T8449 T8450 det the,effect
R4349 T8450 T8448 dobj effect,examine
R4350 T8451 T8450 prep of,effect
R4351 T8452 T8451 pobj IL-6,of
R4352 T8453 T8450 prep on,effect
R4353 T8454 T8455 compound T-cell,responsiveness
R4354 T8455 T8453 pobj responsiveness,on
R4355 T8456 T8455 prep to,responsiveness
R4356 T8457 T8456 pobj IL-10,to
R4357 T8458 T8457 punct ",",IL-10
R4358 T8459 T8462 compound CD4,cells
R4359 T8460 T8462 compound +,cells
R4360 T8461 T8462 compound T,cells
R4361 T8462 T8455 conj cells,responsiveness
R4362 T8463 T8448 prep from,examine
R4363 T8464 T8465 amod healthy,controls
R4364 T8465 T8463 pobj controls,from
R4365 T8466 T8467 auxpass were,incubated
R4366 T8467 T8467 ROOT incubated,incubated
R4367 T8468 T8467 prep in,incubated
R4368 T8469 T8470 compound culture,medium
R4369 T8470 T8468 pobj medium,in
R4370 T8471 T8467 prep with,incubated
R4371 T8472 T8473 nummod 10,%
R4372 T8473 T8474 compound %,FCS
R4373 T8474 T8471 pobj FCS,with
R4374 T8475 T8467 prep in,incubated
R4375 T8476 T8477 det the,presence
R4376 T8477 T8475 pobj presence,in
R4377 T8478 T8477 cc or,presence
R4378 T8479 T8477 conj absence,presence
R4379 T8480 T8479 prep of,absence
R4380 T8481 T8483 nummod 10,IL-6
R4381 T8482 T8483 compound ng/ml,IL-6
R4382 T8483 T8480 pobj IL-6,of
R4383 T8484 T8486 punct (,Dickinson
R4384 T8485 T8486 compound Becton,Dickinson
R4385 T8486 T8483 appos Dickinson,IL-6
R4386 T8487 T8467 punct ),incubated
R4387 T8488 T8467 prep for,incubated
R4388 T8489 T8490 nummod 36,hours
R4389 T8490 T8488 pobj hours,for
R4390 T8491 T8467 punct .,incubated
R4391 T8492 T8495 nsubjpass Cells,stimulated
R4392 T8493 T8495 auxpass were,stimulated
R4393 T8494 T8495 advmod then,stimulated
R4394 T8495 T8495 ROOT stimulated,stimulated
R4395 T8496 T8495 prep for,stimulated
R4396 T8497 T8498 nummod 36,hours
R4397 T8498 T8496 pobj hours,for
R4398 T8499 T8495 prep with,stimulated
R4399 T8500 T8501 compound anti-CD3,mAb
R4400 T8501 T8499 pobj mAb,with
R4401 T8502 T8501 cc and,mAb
R4402 T8503 T8504 amod anti-CD28,mAb
R4403 T8504 T8501 conj mAb,mAb
R4404 T8505 T8504 prep in,mAb
R4405 T8506 T8507 det the,presence
R4406 T8507 T8505 pobj presence,in
R4407 T8508 T8507 cc or,presence
R4408 T8509 T8507 conj absence,presence
R4409 T8510 T8507 prep of,presence
R4410 T8511 T8513 nummod 1,IL-10
R4411 T8512 T8513 compound ng/ml,IL-10
R4412 T8513 T8510 pobj IL-10,of
R4413 T8514 T8495 punct .,stimulated
R4414 T8515 T8516 compound Culture,supernatants
R4415 T8516 T8518 nsubjpass supernatants,measured
R4416 T8517 T8518 auxpass were,measured
R4417 T8518 T8518 ROOT measured,measured
R4418 T8519 T8518 prep for,measured
R4419 T8520 T8521 amod IFN-γ,concentrations
R4420 T8521 T8519 pobj concentrations,for
R4421 T8522 T8518 punct .,measured
R4540 T8847 T8849 nmod Flow,analysis
R4541 T8848 T8849 amod cytometric,analysis
R4542 T8849 T8863 nsubjpass analysis,resuspended
R4543 T8850 T8849 prep for,analysis
R4544 T8851 T8852 compound IL-10R1,expression
R4545 T8852 T8850 pobj expression,for
R4546 T8853 T8854 det A,sample
R4547 T8854 T8863 nsubjpass sample,resuspended
R4548 T8855 T8854 prep of,sample
R4549 T8856 T8859 nummod 5,cells
R4550 T8857 T8859 nummod ×,cells
R4551 T8858 T8859 nummod 105,cells
R4552 T8859 T8855 pobj cells,of
R4553 T8860 T8859 prep of,cells
R4554 T8861 T8860 pobj PBMC,of
R4555 T8862 T8863 auxpass was,resuspended
R4556 T8863 T8863 ROOT resuspended,resuspended
R4557 T8864 T8863 prep in,resuspended
R4558 T8865 T8864 pobj PBS,in
R4559 T8866 T8863 prep with,resuspended
R4560 T8867 T8868 nummod 1,%
R4561 T8868 T8869 compound %,FCS
R4562 T8869 T8866 pobj FCS,with
R4563 T8870 T8863 punct .,resuspended
R4564 T8871 T8873 nsubjpass PBMC,incubated
R4565 T8872 T8873 auxpass were,incubated
R4566 T8873 T8884 ccomp incubated,systems
R4567 T8874 T8873 prep with,incubated
R4568 T8875 T8874 pcomp saturating,with
R4569 T8876 T8875 dobj concentrations,saturating
R4570 T8877 T8876 prep of,concentrations
R4571 T8878 T8879 compound anti-IL-10R1,mAb
R4572 T8879 T8877 pobj mAb,of
R4573 T8880 T8881 punct (,IgG1
R4574 T8881 T8879 appos IgG1,mAb
R4575 T8882 T8884 punct ;,systems
R4576 T8883 T8884 compound R&D,systems
R4577 T8884 T8884 ROOT systems,systems
R4578 T8885 T8884 punct ",",systems
R4579 T8886 T8884 appos Minneapolis,systems
R4580 T8887 T8886 punct ",",Minneapolis
R4581 T8888 T8886 conj MN,Minneapolis
R4582 T8889 T8888 punct ",",MN
R4583 T8890 T8888 conj USA,MN
R4584 T8891 T8888 punct ),MN
R4585 T8892 T8884 cc or,systems
R4586 T8893 T8884 prep with,systems
R4587 T8894 T8895 amod isotype-matched,control
R4588 T8895 T8896 compound control,mAb
R4589 T8896 T8893 pobj mAb,with
R4590 T8897 T8898 punct (,Immunotech
R4591 T8898 T8896 appos Immunotech,mAb
R4592 T8899 T8898 punct ),Immunotech
R4593 T8900 T8901 punct ",",followed
R4594 T8901 T8896 acl followed,mAb
R4595 T8902 T8901 agent by,followed
R4596 T8903 T8902 pobj incubation,by
R4597 T8904 T8903 prep with,incubation
R4598 T8905 T8910 amod FITC-conjugated,antibody
R4599 T8906 T8910 nmod goat,antibody
R4600 T8907 T8910 nmod anti-mouse,antibody
R4601 T8908 T8910 nmod IgG1,antibody
R4602 T8909 T8910 compound polyclonal,antibody
R4603 T8910 T8904 pobj antibody,with
R4604 T8911 T8910 punct (,antibody
R4605 T8912 T8914 compound Santa,Biotechnologies
R4606 T8913 T8914 compound Cruz,Biotechnologies
R4607 T8914 T8910 appos Biotechnologies,antibody
R4608 T8915 T8914 punct ",",Biotechnologies
R4609 T8916 T8917 compound Santa,Cruz
R4610 T8917 T8914 conj Cruz,Biotechnologies
R4611 T8918 T8917 punct ",",Cruz
R4612 T8919 T8917 conj CA,Cruz
R4613 T8920 T8919 punct ",",CA
R4614 T8921 T8919 conj USA,CA
R4615 T8922 T8910 punct ),antibody
R4616 T8923 T8884 punct .,systems
R4617 T8924 T8927 nsubjpass Cells,incubated
R4618 T8925 T8927 auxpass were,incubated
R4619 T8926 T8927 advmod then,incubated
R4620 T8927 T8927 ROOT incubated,incubated
R4621 T8928 T8927 prep with,incubated
R4622 T8929 T8931 amod phycoerythrin-conjugated,mAb
R4623 T8930 T8931 compound anti-CD4,mAb
R4624 T8931 T8928 pobj mAb,with
R4625 T8932 T8931 punct (,mAb
R4626 T8933 T8934 compound Becton,Dickinson
R4627 T8934 T8931 appos Dickinson,mAb
R4628 T8935 T8931 punct ),mAb
R4629 T8936 T8927 punct .,incubated
R4630 T8937 T8939 nsubjpass Cells,washed
R4631 T8938 T8939 auxpass were,washed
R4632 T8939 T8939 ROOT washed,washed
R4633 T8940 T8939 advmod well,washed
R4634 T8941 T8939 prep with,washed
R4635 T8942 T8943 nummod 1,%
R4636 T8943 T8944 compound %,FCS/PBS
R4637 T8944 T8941 pobj FCS/PBS,with
R4638 T8945 T8944 prep between,FCS/PBS
R4639 T8946 T8945 pobj incubations,between
R4640 T8947 T8939 punct .,washed
R4641 T8948 T8950 nsubjpass Analysis,performed
R4642 T8949 T8950 auxpass was,performed
R4643 T8950 T8950 ROOT performed,performed
R4644 T8951 T8950 prep on,performed
R4645 T8952 T8954 det a,flow
R4646 T8953 T8954 amod FACScan,flow
R4647 T8954 T8951 pobj flow,on
R4648 T8955 T8950 npadvmod cytometer,performed
R4649 T8956 T8958 punct (,Dickinson
R4650 T8957 T8958 compound Becton,Dickinson
R4651 T8958 T8955 appos Dickinson,cytometer
R4652 T8959 T8955 punct ),cytometer
R4653 T8960 T8950 punct .,performed
R4715 T9185 T9204 nsubjpass Immunoassay,measured
R4716 T9186 T9185 prep for,Immunoassay
R4717 T9187 T9186 pobj IFN-γ,for
R4718 T9188 T9187 cc and,IFN-γ
R4719 T9189 T9187 conj IL-2,IFN-γ
R4720 T9190 T9186 pobj Concentrations,for
R4721 T9191 T9190 prep of,Concentrations
R4722 T9192 T9191 pobj IFN-γ,of
R4723 T9193 T9192 cc and,IFN-γ
R4724 T9194 T9192 conj IL-2,IFN-γ
R4725 T9195 T9185 prep in,Immunoassay
R4726 T9196 T9197 compound culture,supernatants
R4727 T9197 T9195 pobj supernatants,in
R4728 T9198 T9197 prep of,supernatants
R4729 T9199 T9202 compound CD4,cells
R4730 T9200 T9202 compound +,cells
R4731 T9201 T9202 compound T,cells
R4732 T9202 T9198 pobj cells,of
R4733 T9203 T9204 auxpass were,measured
R4734 T9204 T9204 ROOT measured,measured
R4735 T9205 T9204 prep in,measured
R4736 T9206 T9205 pobj duplicate,in
R4737 T9207 T9204 agent by,measured
R4738 T9208 T9210 det the,sandwich
R4739 T9209 T9210 amod quantitative,sandwich
R4740 T9210 T9211 compound sandwich,ELISA
R4741 T9211 T9207 pobj ELISA,by
R4742 T9212 T9211 acl using,ELISA
R4743 T9213 T9214 amod cytokine-specific,capture
R4744 T9214 T9212 dobj capture,using
R4745 T9215 T9214 prep with,capture
R4746 T9216 T9217 amod biotinylated,detection
R4747 T9217 T9218 compound detection,mAb
R4748 T9218 T9215 pobj mAb,with
R4749 T9219 T9218 cc and,mAb
R4750 T9220 T9222 amod recombinant,proteins
R4751 T9221 T9222 compound cytokine,proteins
R4752 T9222 T9218 conj proteins,mAb
R4753 T9223 T9222 punct (,proteins
R4754 T9224 T9225 advmod all,from
R4755 T9225 T9218 prep from,mAb
R4756 T9226 T9227 compound Becton,Dickinson
R4757 T9227 T9225 pobj Dickinson,from
R4758 T9228 T9218 punct ),mAb
R4759 T9229 T9204 punct ",",measured
R4760 T9230 T9204 prep according,measured
R4761 T9231 T9230 prep to,according
R4762 T9232 T9233 det the,manufacturer
R4763 T9233 T9235 poss manufacturer,protocol
R4764 T9234 T9233 case 's,manufacturer
R4765 T9235 T9231 pobj protocol,to
R4766 T9236 T9204 punct .,measured
R4767 T9237 T9239 det The,limits
R4768 T9238 T9239 compound detection,limits
R4769 T9239 T9244 nsubj limits,were
R4770 T9240 T9239 prep for,limits
R4771 T9241 T9240 pobj IFN-γ,for
R4772 T9242 T9241 cc and,IFN-γ
R4773 T9243 T9241 conj IL-2,IFN-γ
R4774 T9244 T9244 ROOT were,were
R4775 T9245 T9246 nummod 15,pg/ml
R4776 T9246 T9244 attr pg/ml,were
R4777 T9247 T9244 punct .,were
R5108 T10126 T10136 nsubjpass Isolation,extracted
R5109 T10127 T10126 prep of,Isolation
R5110 T10128 T10127 pobj mRNA,of
R5111 T10129 T10128 cc and,mRNA
R5112 T10130 T10134 amod real-time,RNA
R5113 T10131 T10134 compound PCR,RNA
R5114 T10132 T10134 compound Total,RNA
R5115 T10133 T10134 amod cellular,RNA
R5116 T10134 T10128 conj RNA,mRNA
R5117 T10135 T10136 auxpass was,extracted
R5118 T10136 T10136 ROOT extracted,extracted
R5119 T10137 T10136 prep from,extracted
R5120 T10138 T10139 compound PB,CD4
R5121 T10139 T10137 pobj CD4,from
R5122 T10140 T10136 advmod +,extracted
R5123 T10141 T10142 compound T,cells
R5124 T10142 T10140 dobj cells,+
R5125 T10143 T10140 advcl using,+
R5126 T10144 T10147 det an,kit
R5127 T10145 T10147 compound RNA,kit
R5128 T10146 T10147 compound isolation,kit
R5129 T10147 T10143 dobj kit,using
R5130 T10148 T10147 punct (,kit
R5131 T10149 T10150 compound RNeasy,Mini
R5132 T10150 T10151 compound Mini,kit
R5133 T10151 T10147 appos kit,kit
R5134 T10152 T10147 punct ;,kit
R5135 T10153 T10147 appos Qiagen,kit
R5136 T10154 T10153 punct ",",Qiagen
R5137 T10155 T10153 conj Valencia,Qiagen
R5138 T10156 T10155 punct ",",Valencia
R5139 T10157 T10155 conj CA,Valencia
R5140 T10158 T10157 punct ",",CA
R5141 T10159 T10157 conj USA,CA
R5142 T10160 T10157 punct ),CA
R5143 T10161 T10140 punct ",",+
R5144 T10162 T10140 prep according,+
R5145 T10163 T10162 prep to,according
R5146 T10164 T10165 det the,manufacturer
R5147 T10165 T10167 poss manufacturer,instructions
R5148 T10166 T10165 case 's,manufacturer
R5149 T10167 T10163 pobj instructions,to
R5150 T10168 T10136 punct .,extracted
R5151 T10169 T10171 nsubjpass cDNA,synthesized
R5152 T10170 T10171 auxpass was,synthesized
R5153 T10171 T10171 ROOT synthesized,synthesized
R5154 T10172 T10171 prep from,synthesized
R5155 T10173 T10174 amod total,RNA
R5156 T10174 T10172 pobj RNA,from
R5157 T10175 T10174 prep with,RNA
R5158 T10176 T10179 compound Molony,virus
R5159 T10177 T10179 compound murine,virus
R5160 T10178 T10179 compound leukemia,virus
R5161 T10179 T10175 pobj virus,with
R5162 T10180 T10171 prep reverse,synthesized
R5163 T10181 T10180 npadvmod transcriptase,reverse
R5164 T10182 T10184 punct (,Biochemical
R5165 T10183 T10184 compound US,Biochemical
R5166 T10184 T10181 appos Biochemical,transcriptase
R5167 T10185 T10184 punct ",",Biochemical
R5168 T10186 T10184 appos Cleveland,Biochemical
R5169 T10187 T10186 punct ",",Cleveland
R5170 T10188 T10186 conj OH,Cleveland
R5171 T10189 T10188 punct ",",OH
R5172 T10190 T10188 conj USA,OH
R5173 T10191 T10188 punct ),OH
R5174 T10192 T10188 cc and,OH
R5175 T10193 T10184 punct oligo,Biochemical
R5176 T10194 T10193 punct -,oligo
R5177 T10195 T10196 punct (,dT
R5178 T10196 T10199 nmod dT,primers
R5179 T10197 T10196 punct ),dT
R5180 T10198 T10199 nummod 15,primers
R5181 T10199 T10184 appos primers,Biochemical
R5182 T10200 T10201 punct (,Promega
R5183 T10201 T10184 appos Promega,Biochemical
R5184 T10202 T10201 punct ",",Promega
R5185 T10203 T10201 conj Madison,Promega
R5186 T10204 T10203 punct ",",Madison
R5187 T10205 T10203 conj WI,Madison
R5188 T10206 T10205 punct ",",WI
R5189 T10207 T10205 conj USA,WI
R5190 T10208 T10201 punct ),Promega
R5191 T10209 T10171 punct .,synthesized
R5192 T10210 T10211 compound Real-time,PCR
R5193 T10211 T10213 nsubjpass PCR,performed
R5194 T10212 T10213 auxpass was,performed
R5195 T10213 T10213 ROOT performed,performed
R5196 T10214 T10213 prep with,performed
R5197 T10215 T10217 det the,Instrument
R5198 T10216 T10217 compound LightCycler,Instrument
R5199 T10217 T10214 pobj Instrument,with
R5200 T10218 T10217 punct (,Instrument
R5201 T10219 T10220 compound Roche,Diagnostics
R5202 T10220 T10217 conj Diagnostics,Instrument
R5203 T10221 T10220 punct ",",Diagnostics
R5204 T10222 T10220 conj Penzberg,Diagnostics
R5205 T10223 T10222 punct ",",Penzberg
R5206 T10224 T10222 appos Germany,Penzberg
R5207 T10225 T10222 punct ),Penzberg
R5208 T10226 T10213 prep in,performed
R5209 T10227 T10228 compound glass,capillaries
R5210 T10228 T10226 pobj capillaries,in
R5211 T10229 T10213 punct .,performed
R5212 T10230 T10232 det The,mix
R5213 T10231 T10232 compound reaction,mix
R5214 T10232 T10260 nsubjpass mix,added
R5215 T10233 T10232 acl containing,mix
R5216 T10234 T10236 compound Taq,polymerase
R5217 T10235 T10236 compound DNA,polymerase
R5218 T10236 T10233 dobj polymerase,containing
R5219 T10237 T10236 cc and,polymerase
R5220 T10238 T10241 compound DNA,Green
R5221 T10239 T10241 compound double-strand-specific,Green
R5222 T10240 T10241 compound SYBR,Green
R5223 T10241 T10232 appos Green,mix
R5224 T10242 T10243 nsubj I,dye
R5225 T10243 T10232 relcl dye,mix
R5226 T10244 T10243 punct (,dye
R5227 T10245 T10246 compound Lightcycler,FastStart
R5228 T10246 T10250 compound FastStart,Green
R5229 T10247 T10250 compound DNA,Green
R5230 T10248 T10250 compound Master,Green
R5231 T10249 T10250 compound SYBR,Green
R5232 T10250 T10260 nsubjpass Green,added
R5233 T10251 T10250 nmod I,Green
R5234 T10252 T10254 punct ;,Diagnostics
R5235 T10253 T10254 compound Roche,Diagnostics
R5236 T10254 T10250 appos Diagnostics,Green
R5237 T10255 T10254 punct ),Diagnostics
R5238 T10256 T10254 cc and,Diagnostics
R5239 T10257 T10258 amod specific,primers
R5240 T10258 T10254 conj primers,Diagnostics
R5241 T10259 T10260 auxpass were,added
R5242 T10260 T10260 ROOT added,added
R5243 T10261 T10260 prep to,added
R5244 T10262 T10263 compound cDNA,dilutions
R5245 T10263 T10261 pobj dilutions,to
R5246 T10264 T10260 punct .,added
R5247 T10265 T10267 det The,samples
R5248 T10266 T10267 compound cDNA,samples
R5249 T10267 T10269 nsubjpass samples,denatured
R5250 T10268 T10269 auxpass were,denatured
R5251 T10269 T10269 ROOT denatured,denatured
R5252 T10270 T10269 prep at,denatured
R5253 T10271 T10272 nummod 95,°
R5254 T10272 T10270 pobj °,at
R5255 T10273 T10269 conj C,denatured
R5256 T10274 T10273 prep for,C
R5257 T10275 T10276 nummod 10,min
R5258 T10276 T10274 pobj min,for
R5259 T10277 T10273 punct ",",C
R5260 T10278 T10269 cc and,denatured
R5261 T10279 T10281 auxpass were,amplified
R5262 T10280 T10281 advmod then,amplified
R5263 T10281 T10269 conj amplified,denatured
R5264 T10282 T10281 prep for,amplified
R5265 T10283 T10287 preconj 40,at
R5266 T10284 T10285 nummod 50,cycles
R5267 T10285 T10283 appos cycles,40
R5268 T10286 T10287 punct :,at
R5269 T10287 T10281 prep at,amplified
R5270 T10288 T10290 nummod 95,C
R5271 T10289 T10290 nummod °,C
R5272 T10290 T10287 pobj C,at
R5273 T10291 T10290 punct (,C
R5274 T10292 T10293 nummod 10,s
R5275 T10293 T10290 appos s,C
R5276 T10294 T10290 punct ),C
R5277 T10295 T10290 punct ",",C
R5278 T10296 T10287 prep at,at
R5279 T10297 T10299 nummod 65,C
R5280 T10298 T10299 nummod °,C
R5281 T10299 T10296 pobj C,at
R5282 T10300 T10299 punct (,C
R5283 T10301 T10302 nummod 15,s
R5284 T10302 T10299 appos s,C
R5285 T10303 T10299 punct ),C
R5286 T10304 T10287 punct ",",at
R5287 T10305 T10287 cc and,at
R5288 T10306 T10308 nummod 72,C
R5289 T10307 T10308 compound °,C
R5290 T10308 T10287 conj C,at
R5291 T10309 T10310 punct (,22
R5292 T10310 T10308 parataxis 22,C
R5293 T10311 T10308 appos s,C
R5294 T10312 T10308 punct ),C
R5295 T10313 T10308 prep for,C
R5296 T10314 T10313 pobj β-actin,for
R5297 T10315 T10343 quantmod ;,for
R5298 T10316 T10315 nmod at,;
R5299 T10317 T10319 nummod 95,C
R5300 T10318 T10319 nummod °,C
R5301 T10319 T10316 pobj C,at
R5302 T10320 T10319 punct (,C
R5303 T10321 T10322 nummod 10,s
R5304 T10322 T10319 appos s,C
R5305 T10323 T10319 punct ),C
R5306 T10324 T10319 punct ",",C
R5307 T10325 T10316 prep at,at
R5308 T10326 T10328 nummod 62,C
R5309 T10327 T10328 nummod °,C
R5310 T10328 T10325 pobj C,at
R5311 T10329 T10328 punct (,C
R5312 T10330 T10331 nummod 15,s
R5313 T10331 T10328 appos s,C
R5314 T10332 T10328 punct ),C
R5315 T10333 T10316 punct ",",at
R5316 T10334 T10316 cc and,at
R5317 T10335 T10316 conj at,at
R5318 T10336 T10338 nummod 72,C
R5319 T10337 T10338 nummod °,C
R5320 T10338 T10335 pobj C,at
R5321 T10339 T10338 punct (,C
R5322 T10340 T10341 nummod 10,s
R5323 T10341 T10338 appos s,C
R5324 T10342 T10338 punct ),C
R5325 T10343 T10281 prep for,amplified
R5326 T10344 T10343 pobj SOCS1,for
R5327 T10345 T10343 punct ;,for
R5328 T10346 T10343 cc and,for
R5329 T10347 T10343 conj at,for
R5330 T10348 T10350 nummod 96,C
R5331 T10349 T10350 nummod °,C
R5332 T10350 T10347 pobj C,at
R5333 T10351 T10350 punct (,C
R5334 T10352 T10353 nummod 10,s
R5335 T10353 T10350 appos s,C
R5336 T10354 T10350 punct ),C
R5337 T10355 T10350 punct ",",C
R5338 T10356 T10343 conj at,for
R5339 T10357 T10359 nummod 68,C
R5340 T10358 T10359 nummod °,C
R5341 T10359 T10356 pobj C,at
R5342 T10360 T10359 punct (,C
R5343 T10361 T10362 nummod 15,s
R5344 T10362 T10359 appos s,C
R5345 T10363 T10359 punct ),C
R5346 T10364 T10356 punct ",",at
R5347 T10365 T10356 cc and,at
R5348 T10366 T10356 conj at,at
R5349 T10367 T10369 nummod 72,C
R5350 T10368 T10369 nummod °,C
R5351 T10369 T10366 pobj C,at
R5352 T10370 T10369 punct (,C
R5353 T10371 T10372 nummod 15,s
R5354 T10372 T10369 appos s,C
R5355 T10373 T10369 punct ),C
R5356 T10374 T10343 conj for,for
R5357 T10375 T10374 pobj SOCS3,for
R5358 T10376 T10343 punct .,for
R5359 T10377 T10378 compound Amplification,curves
R5360 T10378 T10387 nsubjpass curves,obtained
R5361 T10379 T10378 prep of,curves
R5362 T10380 T10382 det the,values
R5363 T10381 T10382 compound fluorescence,values
R5364 T10382 T10379 pobj values,of
R5365 T10383 T10382 cc versus,values
R5366 T10384 T10385 compound cycle,number
R5367 T10385 T10387 nsubjpass number,obtained
R5368 T10386 T10387 auxpass were,obtained
R5369 T10387 T10387 ROOT obtained,obtained
R5370 T10388 T10387 punct ",",obtained
R5371 T10389 T10387 cc and,obtained
R5372 T10390 T10393 det a,analysis
R5373 T10391 T10393 compound melting,analysis
R5374 T10392 T10393 compound curve,analysis
R5375 T10393 T10396 nsubjpass analysis,performed
R5376 T10394 T10396 auxpass was,performed
R5377 T10395 T10396 advmod then,performed
R5378 T10396 T10387 conj performed,obtained
R5379 T10397 T10396 punct .,performed
R5380 T10398 T10399 det The,levels
R5381 T10399 T10406 nsubjpass levels,determined
R5382 T10400 T10399 prep of,levels
R5383 T10401 T10400 pobj SOCS1,of
R5384 T10402 T10401 cc and,SOCS1
R5385 T10403 T10401 conj SOCS3,SOCS1
R5386 T10404 T10400 pobj expression,of
R5387 T10405 T10406 auxpass were,determined
R5388 T10406 T10406 ROOT determined,determined
R5389 T10407 T10406 prep by,determined
R5390 T10408 T10407 pcomp normalizing,by
R5391 T10409 T10408 advmod relative,normalizing
R5392 T10410 T10409 prep to,relative
R5393 T10411 T10412 compound β-actin,expression
R5394 T10412 T10410 pobj expression,to
R5395 T10413 T10406 punct .,determined
R5396 T10414 T10415 det The,forward
R5397 T10415 T10419 nsubj forward,were
R5398 T10416 T10415 cc and,forward
R5399 T10417 T10415 conj reverse,forward
R5400 T10418 T10417 dobj primers,reverse
R5401 T10419 T10419 ROOT were,were
R5402 T10420 T10421 mark as,follows
R5403 T10421 T10419 advcl follows,were
R5404 T10422 T10419 punct :,were
R5405 T10423 T10419 prep for,were
R5406 T10424 T10423 pobj β-actin,for
R5407 T10425 T10424 punct ",",β-actin
R5408 T10426 T10424 nummod 5,β-actin
R5409 T10427 T10424 punct ',β-actin
R5410 T10428 T10429 punct -,GTGGGGCGCCCCAGGCACCA-3
R5411 T10429 T10424 appos GTGGGGCGCCCCAGGCACCA-3,β-actin
R5412 T10430 T10429 punct ',GTGGGGCGCCCCAGGCACCA-3
R5413 T10431 T10429 cc and,GTGGGGCGCCCCAGGCACCA-3
R5414 T10432 T10435 nummod 5,CTCCTTAATGTCACGCACGATTTC-3
R5415 T10433 T10432 punct ',5
R5416 T10434 T10435 punct -,CTCCTTAATGTCACGCACGATTTC-3
R5417 T10435 T10424 appos CTCCTTAATGTCACGCACGATTTC-3,β-actin
R5418 T10436 T10435 case ',CTCCTTAATGTCACGCACGATTTC-3
R5419 T10437 T10438 preconj ;,for
R5420 T10438 T10419 prep for,were
R5421 T10439 T10438 pobj SOCS1,for
R5422 T10440 T10439 punct ",",SOCS1
R5423 T10441 T10444 nummod 5,AGACCCCTTCTCACCTCTTG-3
R5424 T10442 T10444 punct ',AGACCCCTTCTCACCTCTTG-3
R5425 T10443 T10444 punct -,AGACCCCTTCTCACCTCTTG-3
R5426 T10444 T10439 appos AGACCCCTTCTCACCTCTTG-3,SOCS1
R5427 T10445 T10444 punct ',AGACCCCTTCTCACCTCTTG-3
R5428 T10446 T10444 cc and,AGACCCCTTCTCACCTCTTG-3
R5429 T10447 T10450 nummod 5,GCACAGCAGAAAAATAAAGC-3
R5430 T10448 T10447 punct ',5
R5431 T10449 T10450 punct -,GCACAGCAGAAAAATAAAGC-3
R5432 T10450 T10444 conj GCACAGCAGAAAAATAAAGC-3,AGACCCCTTCTCACCTCTTG-3
R5433 T10451 T10444 punct ',AGACCCCTTCTCACCTCTTG-3
R5434 T10452 T10444 punct ;,AGACCCCTTCTCACCTCTTG-3
R5435 T10453 T10438 cc and,for
R5436 T10454 T10438 conj for,for
R5437 T10455 T10454 pobj SOCS3,for
R5438 T10456 T10455 punct ",",SOCS3
R5439 T10457 T10460 nummod 5,CCCGCCGGCACCTTTCTG-3
R5440 T10458 T10457 punct ',5
R5441 T10459 T10460 punct -,CCCGCCGGCACCTTTCTG-3
R5442 T10460 T10455 appos CCCGCCGGCACCTTTCTG-3,SOCS3
R5443 T10461 T10460 punct ',CCCGCCGGCACCTTTCTG-3
R5444 T10462 T10460 cc and,CCCGCCGGCACCTTTCTG-3
R5445 T10463 T10466 nummod 5,AGGGGCCGGCTCAACACC-3
R5446 T10464 T10463 punct ',5
R5447 T10465 T10466 punct -,AGGGGCCGGCTCAACACC-3
R5448 T10466 T10460 conj AGGGGCCGGCTCAACACC-3,CCCGCCGGCACCTTTCTG-3
R5449 T10467 T10466 punct ',AGGGGCCGGCTCAACACC-3
R5450 T10468 T10419 punct .,were
R5833 T11423 T11425 amod Western,analysis
R5834 T11424 T11425 compound blot,analysis
R5835 T11425 T11429 compound analysis,cells
R5836 T11426 T11429 compound CD4,cells
R5837 T11427 T11429 compound +,cells
R5838 T11428 T11429 compound T,cells
R5839 T11429 T11431 nsubjpass cells,stimulated
R5840 T11430 T11431 auxpass were,stimulated
R5841 T11431 T11431 ROOT stimulated,stimulated
R5842 T11432 T11431 prep for,stimulated
R5843 T11433 T11434 nummod 20,min
R5844 T11434 T11432 pobj min,for
R5845 T11435 T11434 prep by,min
R5846 T11436 T11438 det the,concentrations
R5847 T11437 T11438 amod indicated,concentrations
R5848 T11438 T11435 pobj concentrations,by
R5849 T11439 T11438 prep of,concentrations
R5850 T11440 T11439 pobj IL-10,of
R5851 T11441 T11440 cc and,IL-10
R5852 T11442 T11440 conj IL-6,IL-10
R5853 T11443 T11438 prep at,concentrations
R5854 T11444 T11445 det a,density
R5855 T11445 T11443 pobj density,at
R5856 T11446 T11445 prep of,density
R5857 T11447 T11450 nummod 5,cells
R5858 T11448 T11450 nummod ×,cells
R5859 T11449 T11450 nummod 105,cells
R5860 T11450 T11446 pobj cells,of
R5861 T11451 T11438 prep in,concentrations
R5862 T11452 T11455 nummod 0.5,medium
R5863 T11453 T11455 amod ml,medium
R5864 T11454 T11455 compound culture,medium
R5865 T11455 T11451 pobj medium,in
R5866 T11456 T11455 prep with,medium
R5867 T11457 T11458 nummod 10,%
R5868 T11458 T11459 compound %,FCS
R5869 T11459 T11456 pobj FCS,with
R5870 T11460 T11431 punct .,stimulated
R5871 T11461 T11462 aux To,examine
R5872 T11462 T11478 advcl examine,stimulated
R5873 T11463 T11464 det the,effect
R5874 T11464 T11462 dobj effect,examine
R5875 T11465 T11464 prep of,effect
R5876 T11466 T11467 compound serum,IL-6
R5877 T11467 T11465 pobj IL-6,of
R5878 T11468 T11464 prep on,effect
R5879 T11469 T11470 compound STAT,phosphorylation
R5880 T11470 T11468 pobj phosphorylation,on
R5881 T11471 T11478 punct ",",stimulated
R5882 T11472 T11476 amod normal,cells
R5883 T11473 T11476 compound CD4,cells
R5884 T11474 T11476 nummod +,cells
R5885 T11475 T11476 compound T,cells
R5886 T11476 T11478 nsubjpass cells,stimulated
R5887 T11477 T11478 auxpass were,stimulated
R5888 T11478 T11478 ROOT stimulated,stimulated
R5889 T11479 T11478 prep for,stimulated
R5890 T11480 T11481 nummod 20,min
R5891 T11481 T11479 pobj min,for
R5892 T11482 T11481 prep with,min
R5893 T11483 T11484 nummod 30,%
R5894 T11484 T11487 nmod %,serum
R5895 T11485 T11487 amod active,serum
R5896 T11486 T11487 compound RA,serum
R5897 T11487 T11482 pobj serum,with
R5898 T11488 T11487 prep in,serum
R5899 T11489 T11490 compound culture,medium
R5900 T11490 T11488 pobj medium,in
R5901 T11491 T11481 prep with,min
R5902 T11492 T11493 nummod 40,μg
R5903 T11493 T11495 compound μg,ml
R5904 T11494 T11495 compound /,ml
R5905 T11495 T11491 pobj ml,with
R5906 T11496 T11495 acl neutralizing,ml
R5907 T11497 T11500 nmod goat,antibody
R5908 T11498 T11500 nmod anti-IL-6,antibody
R5909 T11499 T11500 amod polyclonal,antibody
R5910 T11500 T11496 dobj antibody,neutralizing
R5911 T11501 T11502 punct (,IgG
R5912 T11502 T11500 appos IgG,antibody
R5913 T11503 T11502 punct ;,IgG
R5914 T11504 T11502 conj Techne,IgG
R5915 T11505 T11504 punct ",",Techne
R5916 T11506 T11504 conj Princeton,Techne
R5917 T11507 T11506 punct ",",Princeton
R5918 T11508 T11506 conj NJ,Princeton
R5919 T11509 T11508 punct ",",NJ
R5920 T11510 T11508 conj USA,NJ
R5921 T11511 T11508 punct ),NJ
R5922 T11512 T11500 cc or,antibody
R5923 T11513 T11500 conj control,antibody
R5924 T11514 T11515 amod goat,IgG
R5925 T11515 T11513 dobj IgG,control
R5926 T11516 T11517 punct (,Techne
R5927 T11517 T11515 appos Techne,IgG
R5928 T11518 T11495 punct ),ml
R5929 T11519 T11478 punct .,stimulated
R5930 T11520 T11522 amod Whole,lysates
R5931 T11521 T11522 compound cell,lysates
R5932 T11522 T11524 nsubjpass lysates,prepared
R5933 T11523 T11524 auxpass were,prepared
R5934 T11524 T11524 ROOT prepared,prepared
R5935 T11525 T11524 prep by,prepared
R5936 T11526 T11525 pcomp placing,by
R5937 T11527 T11526 dobj cells,placing
R5938 T11528 T11526 prep in,placing
R5939 T11529 T11533 nummod 100,buffer
R5940 T11530 T11533 compound μl,buffer
R5941 T11531 T11533 compound SDS,buffer
R5942 T11532 T11533 compound lysing,buffer
R5943 T11533 T11528 pobj buffer,in
R5944 T11534 T11542 punct (,]
R5945 T11535 T11537 nummod 62.5,Tris
R5946 T11536 T11537 compound mM,Tris
R5947 T11537 T11542 nmod Tris,]
R5948 T11538 T11540 compound HCl,pH
R5949 T11539 T11540 compound [,pH
R5950 T11540 T11537 appos pH,Tris
R5951 T11541 T11542 nummod 6.8,]
R5952 T11542 T11526 prep ],placing
R5953 T11543 T11542 punct ",",]
R5954 T11544 T11545 nummod 2,%
R5955 T11545 T11546 compound %,SDS
R5956 T11546 T11542 npadvmod SDS,]
R5957 T11547 T11546 punct ",",SDS
R5958 T11548 T11549 nummod 10,%
R5959 T11549 T11550 compound %,glycerol
R5960 T11550 T11546 appos glycerol,SDS
R5961 T11551 T11550 punct ",",glycerol
R5962 T11552 T11553 nummod 50,mM
R5963 T11553 T11554 compound mM,dithiothreitol
R5964 T11554 T11550 conj dithiothreitol,glycerol
R5965 T11555 T11554 punct ",",dithiothreitol
R5966 T11556 T11557 nummod 0.1,%
R5967 T11557 T11550 appos %,glycerol
R5968 T11558 T11559 compound bromphenol,blue
R5969 T11559 T11557 appos blue,%
R5970 T11560 T11559 punct ),blue
R5971 T11561 T11542 punct .,]
R5972 T11562 T11568 advmod Then,fractionated
R5973 T11563 T11566 nummod 20,samples
R5974 T11564 T11566 compound μl,samples
R5975 T11565 T11566 compound protein,samples
R5976 T11566 T11568 nsubjpass samples,fractionated
R5977 T11567 T11568 auxpass were,fractionated
R5978 T11568 T11568 ROOT fractionated,fractionated
R5979 T11569 T11568 prep on,fractionated
R5980 T11570 T11571 nummod 10,%
R5981 T11571 T11573 nmod %,gels
R5982 T11572 T11573 amod SDS-polyacrylamide,gels
R5983 T11573 T11569 pobj gels,on
R5984 T11574 T11568 cc and,fractionated
R5985 T11575 T11576 auxpass were,transferred
R5986 T11576 T11568 conj transferred,fractionated
R5987 T11577 T11576 prep to,transferred
R5988 T11578 T11579 amod nitrocellulose,membranes
R5989 T11579 T11577 pobj membranes,to
R5990 T11580 T11592 punct (,blocked
R5991 T11581 T11592 nsubjpass Amersham,blocked
R5992 T11582 T11581 punct ",",Amersham
R5993 T11583 T11581 conj Buckinghamshire,Amersham
R5994 T11584 T11583 punct ",",Buckinghamshire
R5995 T11585 T11583 conj UK,Buckinghamshire
R5996 T11586 T11583 punct ),Buckinghamshire
R5997 T11587 T11581 punct ",",Amersham
R5998 T11588 T11581 cc and,Amersham
R5999 T11589 T11590 det the,membrane
R6000 T11590 T11581 conj membrane,Amersham
R6001 T11591 T11592 auxpass was,blocked
R6002 T11592 T11576 parataxis blocked,transferred
R6003 T11593 T11592 prep with,blocked
R6004 T11594 T11595 nummod 5,%
R6005 T11595 T11596 nsubj %,skim
R6006 T11596 T11576 conj skim,transferred
R6007 T11597 T11596 dobj milk,skim
R6008 T11598 T11596 prep in,skim
R6009 T11599 T11600 amod Tris-buffered,saline
R6010 T11600 T11598 pobj saline,in
R6011 T11601 T11596 prep with,skim
R6012 T11602 T11603 nummod 0.1,%
R6013 T11603 T11605 quantmod %,20
R6014 T11604 T11605 nummod Tween,20
R6015 T11605 T11601 pobj 20,with
R6016 T11606 T11568 punct .,fractionated
R6017 T11607 T11608 compound Tyrosine,phosphorylation
R6018 T11608 T11614 nsubjpass phosphorylation,detected
R6019 T11609 T11608 prep of,phosphorylation
R6020 T11610 T11609 pobj STAT1,of
R6021 T11611 T11610 cc and,STAT1
R6022 T11612 T11610 conj STAT3,STAT1
R6023 T11613 T11614 auxpass was,detected
R6024 T11614 T11614 ROOT detected,detected
R6025 T11615 T11614 advcl using,detected
R6026 T11616 T11618 amod commercial,kits
R6027 T11617 T11618 amod available,kits
R6028 T11618 T11615 dobj kits,using
R6029 T11619 T11618 punct (,kits
R6030 T11620 T11622 compound Cell,Technology
R6031 T11621 T11622 compound Signaling,Technology
R6032 T11622 T11618 appos Technology,kits
R6033 T11623 T11622 punct ",",Technology
R6034 T11624 T11622 conj Beverly,Technology
R6035 T11625 T11624 punct ",",Beverly
R6036 T11626 T11624 conj MA,Beverly
R6037 T11627 T11626 punct ",",MA
R6038 T11628 T11626 appos USA,MA
R6039 T11629 T11626 punct ),MA
R6040 T11630 T11614 prep according,detected
R6041 T11631 T11630 prep to,according
R6042 T11632 T11633 det the,manufacturer
R6043 T11633 T11635 poss manufacturer,instructions
R6044 T11634 T11633 case 's,manufacturer
R6045 T11635 T11631 pobj instructions,to
R6046 T11636 T11614 punct .,detected
R6047 T11637 T11641 advmod Briefly,was
R6048 T11638 T11641 punct ",",was
R6049 T11639 T11640 det the,membrane
R6050 T11640 T11641 nsubj membrane,was
R6051 T11641 T11641 ROOT was,was
R6052 T11642 T11641 attr incubated,was
R6053 T11643 T11642 prep with,incubated
R6054 T11644 T11645 det the,antibodies
R6055 T11645 T11643 pobj antibodies,with
R6056 T11646 T11648 punct (,IgG
R6057 T11647 T11648 compound rabbit,IgG
R6058 T11648 T11645 appos IgG,antibodies
R6059 T11649 T11642 punct ),incubated
R6060 T11650 T11651 amod anti-STAT1,antibody
R6061 T11651 T11671 dep antibody,diluted
R6062 T11652 T11654 punct ",",tyrosine
R6063 T11653 T11654 amod anti-phosphorylated,tyrosine
R6064 T11654 T11651 appos tyrosine,antibody
R6065 T11655 T11654 nummod 701,tyrosine
R6066 T11656 T11654 prep of,tyrosine
R6067 T11657 T11658 nummod STAT1,antibody
R6068 T11658 T11656 pobj antibody,of
R6069 T11659 T11658 punct ",",antibody
R6070 T11660 T11661 amod anti-STAT3,antibody
R6071 T11661 T11658 conj antibody,antibody
R6072 T11662 T11661 punct ",",antibody
R6073 T11663 T11661 cc and,antibody
R6074 T11664 T11665 compound anti-phosphorylated,tyrosine
R6075 T11665 T11661 conj tyrosine,antibody
R6076 T11666 T11671 acl 705,diluted
R6077 T11667 T11666 prep of,705
R6078 T11668 T11669 nummod STAT3,antibody
R6079 T11669 T11667 pobj antibody,of
R6080 T11670 T11671 punct ",",diluted
R6081 T11671 T11641 conj diluted,was
R6082 T11672 T11673 mark as,recommended
R6083 T11673 T11671 advcl recommended,diluted
R6084 T11674 T11673 prep at,recommended
R6085 T11675 T11674 pobj 1/2000,at
R6086 T11676 T11675 prep with,1/2000
R6087 T11677 T11678 amod Tris-buffered,saline
R6088 T11678 T11676 pobj saline,with
R6089 T11679 T11673 prep with,recommended
R6090 T11680 T11681 nummod 0.1,%
R6091 T11681 T11679 pobj %,with
R6092 T11682 T11683 nummod Tween,20
R6093 T11683 T11684 nummod 20,with
R6094 T11684 T11679 conj with,with
R6095 T11685 T11686 nummod 5,%
R6096 T11686 T11687 compound %,BSA
R6097 T11687 T11684 pobj BSA,with
R6098 T11688 T11671 punct .,diluted
R6099 T11689 T11690 compound Antibody,binding
R6100 T11690 T11692 nsubjpass binding,detected
R6101 T11691 T11692 auxpass was,detected
R6102 T11692 T11692 ROOT detected,detected
R6103 T11693 T11692 agent by,detected
R6104 T11694 T11698 amod horseradish,antibody
R6105 T11695 T11698 amod peroxidase-conjugated,antibody
R6106 T11696 T11698 amod anti-rabbit,antibody
R6107 T11697 T11698 compound IgG,antibody
R6108 T11698 T11693 pobj antibody,by
R6109 T11699 T11698 acl diluted,antibody
R6110 T11700 T11699 prep at,diluted
R6111 T11701 T11700 pobj 1/4000,at
R6112 T11702 T11701 prep with,1/4000
R6113 T11703 T11704 amod Tris-buffered,saline
R6114 T11704 T11702 pobj saline,with
R6115 T11705 T11699 prep with,diluted
R6116 T11706 T11707 nummod 0.1,%
R6117 T11707 T11705 pobj %,with
R6118 T11708 T11709 nummod Tween,20
R6119 T11709 T11707 prep 20,%
R6120 T11710 T11709 prep with,20
R6121 T11711 T11712 nummod 5,%
R6122 T11712 T11713 compound %,BSA
R6123 T11713 T11710 pobj BSA,with
R6124 T11714 T11707 punct ",",%
R6125 T11715 T11692 cc and,detected
R6126 T11716 T11717 auxpass was,revealed
R6127 T11717 T11692 conj revealed,detected
R6128 T11718 T11717 advcl using,revealed
R6129 T11719 T11721 det the,system
R6130 T11720 T11721 compound chemiluminescence,system
R6131 T11721 T11718 dobj system,using
R6132 T11722 T11692 punct .,detected
R6133 T11723 T11724 compound Protein,bands
R6134 T11724 T11726 nsubjpass bands,quantified
R6135 T11725 T11726 auxpass were,quantified
R6136 T11726 T11726 ROOT quantified,quantified
R6137 T11727 T11726 agent by,quantified
R6138 T11728 T11727 pobj densitometry,by
R6139 T11729 T11726 advcl using,quantified
R6140 T11730 T11731 amod NIH-Image,analysis
R6141 T11731 T11729 dobj analysis,using
R6142 T11732 T11726 punct ",",quantified
R6143 T11733 T11726 cc and,quantified
R6144 T11734 T11735 compound STAT,phosphorylation
R6145 T11735 T11736 nsubj phosphorylation,was
R6146 T11736 T11726 conj was,quantified
R6147 T11737 T11736 prep compared,was
R6148 T11738 T11737 prep with,compared
R6149 T11739 T11741 det the,amount
R6150 T11740 T11741 amod total,amount
R6151 T11741 T11738 pobj amount,with
R6152 T11742 T11741 prep of,amount
R6153 T11743 T11744 compound STAT,protein
R6154 T11744 T11742 pobj protein,of
R6155 T11745 T11736 punct .,was
R6156 T11746 T11748 amod IFN-γ-stimulated,cells
R6157 T11747 T11748 compound Hela,cells
R6158 T11748 T11750 nsubjpass cells,used
R6159 T11749 T11750 auxpass were,used
R6160 T11750 T11750 ROOT used,used
R6161 T11751 T11750 prep as,used
R6162 T11752 T11754 det a,control
R6163 T11753 T11754 amod positive,control
R6164 T11754 T11751 pobj control,as
R6165 T11755 T11754 prep for,control
R6166 T11756 T11757 nummod STAT1,phosophorylation
R6167 T11757 T11755 pobj phosophorylation,for
R6168 T11758 T11750 punct .,used
R6255 T12022 T12023 compound Statistical,analysis
R6256 T12023 T12024 compound analysis,Data
R6257 T12024 T12026 nsubjpass Data,expressed
R6258 T12025 T12026 auxpass are,expressed
R6259 T12026 T12026 ROOT expressed,expressed
R6260 T12027 T12026 prep as,expressed
R6261 T12028 T12033 det the,error
R6262 T12029 T12033 amod mean,error
R6263 T12030 T12033 nmod value,error
R6264 T12031 T12033 nmod ±,error
R6265 T12032 T12033 amod standard,error
R6266 T12033 T12027 pobj error,as
R6267 T12034 T12033 prep of,error
R6268 T12035 T12036 det the,mean
R6269 T12036 T12034 pobj mean,of
R6270 T12037 T12036 cc or,mean
R6271 T12038 T12039 compound box,plots
R6272 T12039 T12036 conj plots,mean
R6273 T12040 T12026 punct .,expressed
R6274 T12041 T12043 det The,significance
R6275 T12042 T12043 amod statistical,significance
R6276 T12043 T12050 nsubjpass significance,determined
R6277 T12044 T12043 prep of,significance
R6278 T12045 T12044 pobj differences,of
R6279 T12046 T12045 prep between,differences
R6280 T12047 T12048 nummod two,groups
R6281 T12048 T12046 pobj groups,between
R6282 T12049 T12050 auxpass was,determined
R6283 T12050 T12050 ROOT determined,determined
R6284 T12051 T12050 agent by,determined
R6285 T12052 T12053 det the,Mann
R6286 T12053 T12051 pobj Mann,by
R6287 T12054 T12056 compound Whitney,test
R6288 T12055 T12056 compound U,test
R6289 T12056 T12060 acomp test,signed
R6290 T12057 T12056 cc or,test
R6291 T12058 T12059 det the,Wilcoxon
R6292 T12059 T12060 nsubj Wilcoxon,signed
R6293 T12060 T12050 advcl signed,determined
R6294 T12061 T12062 compound rank,test
R6295 T12062 T12060 dobj test,signed
R6296 T12063 T12050 punct .,determined
R6297 T12064 T12068 nsubjpass P,considered
R6298 T12065 T12066 amod <,0.05
R6299 T12066 T12064 nummod 0.05,P
R6300 T12067 T12068 auxpass was,considered
R6301 T12068 T12068 ROOT considered,considered
R6302 T12069 T12068 oprd significant,considered
R6303 T12070 T12068 punct .,considered
R3587 T7270 T7271 nummod 11.2,years
R3588 T7271 T7269 appos years,±
R3589 T7272 T7258 punct ),volunteers

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T11057 7662-7669 Positive_regulation denotes control
T11056 7680-7696 Phosphorylation denotes phosophorylation
T11055 7674-7679 Protein denotes STAT1
T11054 7610-7615 Protein denotes IFN-γ
T11053 7542-7557 Phosphorylation denotes phosphorylation
T11052 7542-7557 Phosphorylation denotes phosphorylation
T11051 7596-7608 Protein denotes STAT protein
T11050 7537-7541 Protein denotes STAT
T11049 7249-7256 Binding denotes binding
T11048 7273-7331 Protein denotes horseradish peroxidase-conjugated anti-rabbit IgG antibody
T11047 7097-7147 Protein denotes anti-phosphorylated tyrosine 705 of STAT3 antibody
T11046 7072-7091 Protein denotes anti-STAT3 antibody
T11045 7020-7070 Protein denotes anti-phosphorylated tyrosine 701 of STAT1 antibody
T11044 6758-6773 Phosphorylation denotes phosphorylation
T11043 6758-6773 Phosphorylation denotes phosphorylation
T11042 6749-6757 Entity denotes Tyrosine
T11041 6787-6792 Protein denotes STAT3
T11040 6777-6782 Protein denotes STAT1
T11039 6065-6071 Positive_regulation denotes effect
T11038 6215-6227 Negative_regulation denotes neutralizing
T11037 6215-6227 Negative_regulation denotes neutralizing
T11036 6215-6227 Negative_regulation denotes neutralizing
T11035 6094-6109 Phosphorylation denotes phosphorylation
T11034 6313-6316 Protein denotes IgG
T11033 6264-6267 Protein denotes IgG
T11032 6228-6262 Protein denotes goat anti-IL-6 polyclonal antibody
T11031 6118-6122 Protein denotes CD4+
T11030 6089-6093 Protein denotes STAT
T11029 6075-6085 Protein denotes serum IL-6
T11028 5976-5980 Protein denotes IL-6
T11027 5966-5971 Protein denotes IL-10
T11026 5891-5895 Protein denotes CD4+
T9790 5806-5811 Protein denotes SOCS3
T9789 5731-5736 Protein denotes SOCS1
T9788 5654-5661 Protein denotes β-actin
T9787 5589-5599 Gene_expression denotes expression
T9786 5527-5537 Gene_expression denotes expression
T9785 5527-5537 Gene_expression denotes expression
T9784 5581-5588 Protein denotes β-actin
T9783 5521-5526 Protein denotes SOCS3
T9782 5511-5516 Protein denotes SOCS1
T9781 5358-5363 Protein denotes SOCS3
T9780 5289-5294 Protein denotes SOCS1
T9779 5222-5229 Protein denotes β-actin
T9778 4887-4905 Protein denotes Taq DNA polymerase
T9777 4625-4646 Protein denotes reverse transcriptase
T9096 4325-4329 Protein denotes IL-2
T9095 4315-4320 Protein denotes IFN-γ
T9094 4181-4218 Protein denotes mAb and recombinant cytokine proteins
T9093 4046-4050 Protein denotes CD4+
T9092 4014-4018 Protein denotes IL-2
T9091 4004-4009 Protein denotes IFN-γ
T9090 3981-3985 Protein denotes IL-2
T9089 3971-3976 Protein denotes IFN-γ
T8716 3658-3662 Protein denotes IgG1
T8715 3507-3511 Protein denotes IgG1
T8714 3489-3505 Protein denotes anti-IL-10R1 mAb
T8713 3354-3364 Gene_expression denotes expression
T8712 3346-3353 Protein denotes IL-10R1
T8085 3294-3299 Protein denotes IFN-γ
T8084 3196-3209 Protein denotes anti-CD28 mAb
T8083 3179-3191 Protein denotes anti-CD3 mAb
T8082 2976-2979 Protein denotes CD4
T8081 2969-2974 Protein denotes IL-10
T8080 2936-2940 Protein denotes IL-6
T8079 2608-2612 Protein denotes CD28
T8078 2511-2531 Protein denotes 1 μg/ml anti-CD3 mAb
T8077 2297-2300 Protein denotes CD4
T8076 2281-2285 Protein denotes CD4+
T7500 2198-2202 Protein denotes CD4+
T7499 1725-1730 Protein denotes DNase
T7498 1709-1720 Protein denotes collagenase
T7497 1575-1579 Protein denotes CD4+
T7496 1461-1464 Protein denotes CD4
T7495 1391-1395 Protein denotes CD4+
T7494 1205-1209 Protein denotes CD4+
T6872 796-804 Positive_regulation denotes elevated
T6871 805-814 Protein denotes serum CRP
T6870 623-626 Protein denotes IgM
T6869 589-592 Protein denotes CRP
T6868 563-587 Protein denotes serum C-reactive protein
R3192 T6871 T6872 themeOf serum CRP,elevated
R4425 T8712 T8713 themeOf IL-10R1,expression
R4788 T9782 T9785 themeOf SOCS1,expression
R4789 T9783 T9786 themeOf SOCS3,expression
R4790 T9784 T9787 themeOf β-actin,expression
R5485 T11030 T11035 themeOf STAT,phosphorylation
R5486 T11032 T11036 themeOf goat anti-IL-6 polyclonal antibody,neutralizing
R5487 T11033 T11037 themeOf IgG,neutralizing
R5488 T11034 T11038 themeOf IgG,neutralizing
R5489 T11035 T11039 themeOf phosphorylation,effect
R5490 T11040 T11043 themeOf STAT1,phosphorylation
R5491 T11040 T11042 partOf STAT1,Tyrosine
R5492 T11041 T11044 themeOf STAT3,phosphorylation
R5493 T11041 T11042 partOf STAT3,Tyrosine
R5494 T11042 T11043 Site Tyrosine,phosphorylation
R5495 T11042 T11044 Site Tyrosine,phosphorylation
R5496 T11048 T11049 themeOf horseradish peroxidase-conjugated anti-rabbit IgG antibody,binding
R5497 T11050 T11052 themeOf STAT,phosphorylation
R5498 T11051 T11053 themeOf STAT protein,phosphorylation
R5499 T11055 T11056 themeOf STAT1,phosophorylation
R5500 T11056 T11057 themeOf phosophorylation,control
R5484 T11029 T11039 causeOf serum IL-6,effect

test3

Id Subject Object Predicate Lexical cue
T10647 7674-7679 Protein denotes STAT1
T10646 7610-7615 Protein denotes IFN-γ
T10645 7542-7557 Phosphorylation denotes phosphorylation
T10644 7537-7541 Protein denotes STAT
T10643 7249-7256 Binding denotes binding
T10642 7133-7138 Protein denotes STAT3
T10641 7077-7082 Protein denotes STAT3
T10640 7056-7061 Protein denotes STAT1
T10639 7004-7009 Protein denotes STAT1
T10638 6787-6792 Protein denotes STAT3
T10637 6777-6782 Protein denotes STAT1
T10636 6758-6773 Phosphorylation denotes phosphorylation
T10635 6238-6242 Protein denotes IL-6
T10634 6118-6121 Protein denotes CD4
T10633 6094-6109 Phosphorylation denotes phosphorylation
T10632 6081-6085 Protein denotes IL-6
T10631 6065-6071 Regulation denotes effect
T10630 5976-5980 Protein denotes IL-6
T10629 5966-5971 Protein denotes IL-10
T10628 5891-5894 Protein denotes CD4
T10627 7674-7679 Protein denotes STAT1
T10626 7610-7615 Protein denotes IFN-γ
T10625 7537-7541 Protein denotes STAT
T10624 7133-7138 Protein denotes STAT3
T10623 7077-7082 Protein denotes STAT3
T10622 7056-7061 Protein denotes STAT1
T10621 7004-7009 Protein denotes STAT1
T10620 6787-6792 Protein denotes STAT3
T10619 6777-6782 Protein denotes STAT1
T10618 6238-6242 Protein denotes IL-6
T10617 6118-6121 Protein denotes CD4
T10616 6081-6085 Protein denotes IL-6
T10615 5976-5980 Protein denotes IL-6
T10614 5966-5971 Protein denotes IL-10
T10613 5891-5894 Protein denotes CD4
T9354 5806-5811 Protein denotes SOCS3
T9353 5731-5736 Protein denotes SOCS1
T9352 5654-5661 Protein denotes β-actin
T9351 5589-5599 Gene_expression denotes expression
T9350 5581-5588 Protein denotes β-actin
T9349 5527-5537 Gene_expression denotes expression
T9348 5521-5526 Protein denotes SOCS3
T9347 5511-5516 Protein denotes SOCS1
T9346 5358-5363 Protein denotes SOCS3
T9345 5289-5294 Protein denotes SOCS1
T9344 5222-5229 Protein denotes β-actin
T9343 4887-4890 Protein denotes Taq
T9342 4423-4426 Protein denotes CD4
T9341 5806-5811 Protein denotes SOCS3
T9340 5731-5736 Protein denotes SOCS1
T9339 5654-5661 Protein denotes β-actin
T9338 5581-5588 Protein denotes β-actin
T9337 5521-5526 Protein denotes SOCS3
T9336 5511-5516 Protein denotes SOCS1
T9335 5358-5363 Protein denotes SOCS3
T9334 5289-5294 Protein denotes SOCS1
T9333 5222-5229 Protein denotes β-actin
T9332 4887-4890 Protein denotes Taq
T9331 4423-4426 Protein denotes CD4
T9020 4325-4329 Protein denotes IL-2
T9019 4315-4320 Protein denotes IFN-γ
T9018 4046-4049 Protein denotes CD4
T9017 4014-4018 Protein denotes IL-2
T9016 4004-4009 Protein denotes IFN-γ
T9015 3971-3976 Protein denotes IFN-γ
T9014 4325-4329 Protein denotes IL-2
T9013 4315-4320 Protein denotes IFN-γ
T9012 4046-4049 Protein denotes CD4
T9011 4014-4018 Protein denotes IL-2
T9010 4004-4009 Protein denotes IFN-γ
T9009 3981-3985 Protein denotes IL-2
T9008 3971-3976 Protein denotes IFN-γ
T8616 3795-3798 Protein denotes CD4
T8615 3494-3501 Protein denotes IL-10R1
T8614 3354-3364 Gene_expression denotes expression
T8613 3346-3353 Protein denotes IL-10R1
T8612 3795-3798 Protein denotes CD4
T8611 3494-3501 Protein denotes IL-10R1
T8610 3346-3353 Protein denotes IL-10R1
T8015 3294-3299 Protein denotes IFN-γ
T8014 3248-3253 Protein denotes IL-10
T8013 3229-3236 Negative_regulation denotes absence
T8012 3201-3205 Protein denotes CD28
T8011 3096-3100 Protein denotes IL-6
T8010 3076-3083 Negative_regulation denotes absence
T8009 2976-2979 Protein denotes CD4
T8008 2969-2974 Protein denotes IL-10
T8007 2936-2940 Protein denotes IL-6
T8006 2926-2932 Regulation denotes effect
T8005 2692-2697 Protein denotes IL-10
T8004 2649-2656 Negative_regulation denotes absence
T8003 2608-2612 Protein denotes CD28
T8002 2297-2300 Protein denotes CD4
T8001 2281-2284 Protein denotes CD4
T8000 3294-3299 Protein denotes IFN-γ
T7999 3248-3253 Protein denotes IL-10
T7998 3201-3205 Protein denotes CD28
T7997 3096-3100 Protein denotes IL-6
T7996 2976-2979 Protein denotes CD4
T7995 2969-2974 Protein denotes IL-10
T7994 2936-2940 Protein denotes IL-6
T7993 2692-2697 Protein denotes IL-10
T7992 2608-2612 Protein denotes CD28
T7991 2297-2300 Protein denotes CD4
T7990 2281-2284 Protein denotes CD4
T7338 2198-2201 Protein denotes CD4
T7337 1575-1578 Protein denotes CD4
T7336 1461-1464 Protein denotes CD4
T7335 1391-1394 Protein denotes CD4
T7334 1205-1208 Protein denotes CD4
T7333 2198-2201 Protein denotes CD4
T7332 1575-1578 Protein denotes CD4
T7331 1461-1464 Protein denotes CD4
T7330 1391-1394 Protein denotes CD4
T7329 1205-1208 Protein denotes CD4
R4013 T8007 T8006 causeOf IL-6,effect
R4014 T8011 T8010 themeOf IL-6,absence
R4015 T8014 T8013 themeOf IL-10,absence
R4423 T8613 T8614 themeOf IL-10R1,expression
R4781 T9347 T9349 themeOf SOCS1,expression
R4782 T9348 T9349 themeOf SOCS3,expression
R4783 T9350 T9351 themeOf β-actin,expression
R5470 T10637 T10636 themeOf STAT1,phosphorylation
R5471 T10638 T10636 themeOf STAT3,phosphorylation
R5472 T10644 T10645 themeOf STAT,phosphorylation

testone

Id Subject Object Predicate Lexical cue
T10594 7542-7557 Phosphorylation denotes phosphorylation
T10593 6758-6773 Phosphorylation denotes phosphorylation
T10592 6094-6109 Phosphorylation denotes phosphorylation
T10591 6065-6071 Regulation denotes effect
T10590 7674-7679 Protein denotes STAT1
T10589 7610-7615 Protein denotes IFN-γ
T10588 7537-7541 Protein denotes STAT
T10587 7133-7138 Protein denotes STAT3
T10586 7077-7082 Protein denotes STAT3
T10585 7056-7061 Protein denotes STAT1
T10584 7004-7009 Protein denotes STAT1
T10583 6787-6792 Protein denotes STAT3
T10582 6777-6782 Protein denotes STAT1
T10581 6238-6242 Protein denotes IL-6
T10580 6118-6121 Protein denotes CD4
T10579 6081-6085 Protein denotes IL-6
T10578 5976-5980 Protein denotes IL-6
T10577 5966-5971 Protein denotes IL-10
T10576 5891-5894 Protein denotes CD4
T9318 5589-5599 Gene_expression denotes expression
T9317 5527-5537 Gene_expression denotes expression
T9316 5806-5811 Protein denotes SOCS3
T9315 5731-5736 Protein denotes SOCS1
T9314 5654-5661 Protein denotes β-actin
T9313 5581-5588 Protein denotes β-actin
T9312 5521-5526 Protein denotes SOCS3
T9311 5511-5516 Protein denotes SOCS1
T9310 5358-5363 Protein denotes SOCS3
T9309 5289-5294 Protein denotes SOCS1
T9308 5222-5229 Protein denotes β-actin
T9307 4887-4890 Protein denotes Taq
T9306 4423-4426 Protein denotes CD4
T8999 4325-4329 Protein denotes IL-2
T8998 4315-4320 Protein denotes IFN-γ
T8997 4046-4049 Protein denotes CD4
T8996 4014-4018 Protein denotes IL-2
T8995 4004-4009 Protein denotes IFN-γ
T8994 3981-3985 Protein denotes IL-2
T8993 3971-3976 Protein denotes IFN-γ
T8603 3354-3364 Gene_expression denotes expression
T8602 3795-3798 Protein denotes CD4
T8601 3494-3501 Protein denotes IL-10R1
T8600 3346-3353 Protein denotes IL-10R1
T7980 3294-3299 Protein denotes IFN-γ
T7979 3248-3253 Protein denotes IL-10
T7978 3201-3205 Protein denotes CD28
T7977 3096-3100 Protein denotes IL-6
T7976 2976-2979 Protein denotes CD4
T7975 2969-2974 Protein denotes IL-10
T7974 2936-2940 Protein denotes IL-6
T7973 2692-2697 Protein denotes IL-10
T7972 2608-2612 Protein denotes CD28
T7971 2297-2300 Protein denotes CD4
T7970 2281-2284 Protein denotes CD4
T7321 2198-2201 Protein denotes CD4
T7320 1575-1578 Protein denotes CD4
T7319 1461-1464 Protein denotes CD4
T7318 1391-1394 Protein denotes CD4
T7317 1205-1208 Protein denotes CD4
R4422 T8600 T8603 themeOf IL-10R1,expression
R4778 T9311 T9317 themeOf SOCS1,expression
R4779 T9312 T9317 themeOf SOCS3,expression
R4780 T9313 T9318 themeOf β-actin,expression
R5465 T10579 T10592 themeOf IL-6,phosphorylation
R5466 T10582 T10593 themeOf STAT1,phosphorylation
R5467 T10583 T10593 themeOf STAT3,phosphorylation
R5468 T10588 T10594 themeOf STAT,phosphorylation
R5469 T10592 T10591 themeOf phosphorylation,effect