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CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T12908","span":{"begin":862,"end":867},"obj":"Protein"},{"id":"T12907","span":{"begin":808,"end":813},"obj":"Protein"},{"id":"T12906","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T12905","span":{"begin":718,"end":721},"obj":"Protein"},{"id":"T12904","span":{"begin":699,"end":704},"obj":"Protein"},{"id":"T12903","span":{"begin":629,"end":632},"obj":"Protein"},{"id":"T12902","span":{"begin":592,"end":597},"obj":"Protein"},{"id":"T12901","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T12900","span":{"begin":508,"end":513},"obj":"Protein"},{"id":"T12899","span":{"begin":452,"end":456},"obj":"Protein"},{"id":"T12898","span":{"begin":430,"end":433},"obj":"Protein"},{"id":"T12897","span":{"begin":314,"end":317},"obj":"Protein"},{"id":"T12896","span":{"begin":295,"end":300},"obj":"Protein"},{"id":"T12895","span":{"begin":279,"end":282},"obj":"Protein"},{"id":"T12894","span":{"begin":161,"end":165},"obj":"Protein"},{"id":"T12893","span":{"begin":105,"end":110},"obj":"Protein"},{"id":"T12892","span":{"begin":4,"end":8},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"The CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T13111","span":{"begin":430,"end":433},"obj":"http://www.uniprot.org/uniprot/P09693"},{"id":"T13108","span":{"begin":430,"end":433},"obj":"http://www.uniprot.org/uniprot/P07766"},{"id":"T13105","span":{"begin":430,"end":433},"obj":"http://www.uniprot.org/uniprot/P04234"},{"id":"T13102","span":{"begin":430,"end":433},"obj":"http://www.uniprot.org/uniprot/P20963"},{"id":"T13099","span":{"begin":452,"end":456},"obj":"http://www.uniprot.org/uniprot/P10747"},{"id":"T13098","span":{"begin":161,"end":165},"obj":"http://www.uniprot.org/uniprot/P10747"},{"id":"T13097","span":{"begin":4,"end":8},"obj":"http://www.uniprot.org/uniprot/P10747"},{"id":"T13091","span":{"begin":718,"end":721},"obj":"http://www.uniprot.org/uniprot/P01730"},{"id":"T13090","span":{"begin":629,"end":632},"obj":"http://www.uniprot.org/uniprot/P01730"},{"id":"T13089","span":{"begin":314,"end":317},"obj":"http://www.uniprot.org/uniprot/P01730"},{"id":"T13088","span":{"begin":279,"end":282},"obj":"http://www.uniprot.org/uniprot/P01730"},{"id":"T13071","span":{"begin":862,"end":867},"obj":"http://www.uniprot.org/uniprot/P22301"},{"id":"T13070","span":{"begin":808,"end":813},"obj":"http://www.uniprot.org/uniprot/P22301"},{"id":"T13069","span":{"begin":699,"end":704},"obj":"http://www.uniprot.org/uniprot/P22301"},{"id":"T13068","span":{"begin":508,"end":513},"obj":"http://www.uniprot.org/uniprot/P22301"},{"id":"T13067","span":{"begin":295,"end":300},"obj":"http://www.uniprot.org/uniprot/P22301"},{"id":"T13066","span":{"begin":105,"end":110},"obj":"http://www.uniprot.org/uniprot/P22301"},{"id":"T13082","span":{"begin":775,"end":780},"obj":"http://www.uniprot.org/uniprot/P01579"},{"id":"T13081","span":{"begin":592,"end":597},"obj":"http://www.uniprot.org/uniprot/P01579"},{"id":"T13080","span":{"begin":532,"end":537},"obj":"http://www.uniprot.org/uniprot/P01579"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"The CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml."}

{"project":"GO-BP","denotations":[{"id":"T12820","span":{"begin":596,"end":621},"obj":"http://purl.obolibrary.org/obo/GO_0032729"},{"id":"T12819","span":{"begin":175,"end":190},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T12818","span":{"begin":89,"end":110},"obj":"http://purl.obolibrary.org/obo/GO_0032613"},{"id":"T12817","span":{"begin":89,"end":110},"obj":"http://purl.obolibrary.org/obo/GO_0032653"},{"id":"T12816","span":{"begin":80,"end":99},"obj":"http://purl.obolibrary.org/obo/GO_0001816"},{"id":"T12815","span":{"begin":75,"end":99},"obj":"http://purl.obolibrary.org/obo/GO_0035745"},{"id":"T12814","span":{"begin":75,"end":99},"obj":"http://purl.obolibrary.org/obo/GO_0035744"},{"id":"T12813","span":{"begin":75,"end":99},"obj":"http://purl.obolibrary.org/obo/GO_0002368"},{"id":"T12812","span":{"begin":75,"end":99},"obj":"http://purl.obolibrary.org/obo/GO_0032762"},{"id":"T12811","span":{"begin":75,"end":99},"obj":"http://purl.obolibrary.org/obo/GO_0002371"},{"id":"T12810","span":{"begin":75,"end":99},"obj":"http://purl.obolibrary.org/obo/GO_0002370"},{"id":"T12809","span":{"begin":73,"end":99},"obj":"http://purl.obolibrary.org/obo/GO_0002724"},{"id":"T12808","span":{"begin":73,"end":99},"obj":"http://purl.obolibrary.org/obo/GO_0002369"}],"text":"The CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml."}

{"project":"GO-CC","denotations":[{"id":"T12964","span":{"begin":457,"end":465},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T12963","span":{"begin":434,"end":442},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T12962","span":{"begin":457,"end":465},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T12961","span":{"begin":434,"end":442},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T12955","span":{"begin":725,"end":730},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12954","span":{"begin":636,"end":641},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12953","span":{"begin":321,"end":326},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T12952","span":{"begin":286,"end":291},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"The CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml."}

{"project":"GO-MF","denotations":[{"id":"T12948","span":{"begin":457,"end":465},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T12947","span":{"begin":434,"end":442},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T12946","span":{"begin":195,"end":235},"obj":"http://purl.obolibrary.org/obo/GO_0043548"},{"id":"T12945","span":{"begin":195,"end":228},"obj":"http://purl.obolibrary.org/obo/GO_0032266"},{"id":"T12944","span":{"begin":195,"end":202},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T12936","span":{"begin":862,"end":867},"obj":"http://purl.obolibrary.org/obo/GO_0005141"},{"id":"T12935","span":{"begin":808,"end":813},"obj":"http://purl.obolibrary.org/obo/GO_0005141"},{"id":"T12934","span":{"begin":699,"end":704},"obj":"http://purl.obolibrary.org/obo/GO_0005141"},{"id":"T12933","span":{"begin":508,"end":513},"obj":"http://purl.obolibrary.org/obo/GO_0005141"},{"id":"T12932","span":{"begin":295,"end":300},"obj":"http://purl.obolibrary.org/obo/GO_0005141"},{"id":"T12931","span":{"begin":105,"end":110},"obj":"http://purl.obolibrary.org/obo/GO_0005141"}],"text":"The CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml."}

{"project":"sentences","denotations":[{"id":"T12800","span":{"begin":706,"end":897},"obj":"Sentence"},{"id":"T12799","span":{"begin":572,"end":705},"obj":"Sentence"},{"id":"T12798","span":{"begin":242,"end":571},"obj":"Sentence"},{"id":"T12797","span":{"begin":0,"end":241},"obj":"Sentence"},{"id":"T93","span":{"begin":0,"end":241},"obj":"Sentence"},{"id":"T94","span":{"begin":242,"end":571},"obj":"Sentence"},{"id":"T95","span":{"begin":572,"end":705},"obj":"Sentence"},{"id":"T96","span":{"begin":706,"end":897},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml."}

{"project":"simple1","denotations":[{"id":"T13135","span":{"begin":862,"end":867},"obj":"Protein"},{"id":"T13134","span":{"begin":808,"end":813},"obj":"Protein"},{"id":"T13133","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T13132","span":{"begin":718,"end":721},"obj":"Protein"},{"id":"T13131","span":{"begin":699,"end":704},"obj":"Protein"},{"id":"T13130","span":{"begin":629,"end":632},"obj":"Protein"},{"id":"T13129","span":{"begin":592,"end":597},"obj":"Protein"},{"id":"T13128","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T13127","span":{"begin":508,"end":513},"obj":"Protein"},{"id":"T13126","span":{"begin":452,"end":456},"obj":"Protein"},{"id":"T13125","span":{"begin":430,"end":433},"obj":"Protein"},{"id":"T13124","span":{"begin":314,"end":317},"obj":"Protein"},{"id":"T13123","span":{"begin":295,"end":300},"obj":"Protein"},{"id":"T13122","span":{"begin":279,"end":282},"obj":"Protein"},{"id":"T13121","span":{"begin":161,"end":165},"obj":"Protein"},{"id":"T13120","span":{"begin":105,"end":110},"obj":"Protein"},{"id":"T13119","span":{"begin":4,"end":8},"obj":"Protein"}],"text":"The CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T14399","span":{"begin":744,"end":751},"obj":"Gene_expression"},{"id":"T14398","span":{"begin":653,"end":662},"obj":"Negative_regulation"},{"id":"T14397","span":{"begin":598,"end":608},"obj":"Gene_expression"},{"id":"T14396","span":{"begin":538,"end":548},"obj":"Gene_expression"},{"id":"T14395","span":{"begin":175,"end":190},"obj":"Phosphorylation"},{"id":"T14394","span":{"begin":150,"end":160},"obj":"Negative_regulation"},{"id":"T14370","span":{"begin":862,"end":867},"obj":"Protein"},{"id":"T14369","span":{"begin":808,"end":813},"obj":"Protein"},{"id":"T14368","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T14367","span":{"begin":718,"end":721},"obj":"Protein"},{"id":"T14366","span":{"begin":699,"end":704},"obj":"Protein"},{"id":"T14365","span":{"begin":629,"end":632},"obj":"Protein"},{"id":"T14364","span":{"begin":592,"end":597},"obj":"Protein"},{"id":"T14363","span":{"begin":314,"end":317},"obj":"Protein"},{"id":"T14362","span":{"begin":295,"end":300},"obj":"Protein"},{"id":"T14361","span":{"begin":279,"end":282},"obj":"Protein"},{"id":"T14360","span":{"begin":532,"end":537},"obj":"Protein"},{"id":"T14359","span":{"begin":508,"end":513},"obj":"Protein"},{"id":"T14358","span":{"begin":452,"end":456},"obj":"Protein"},{"id":"T14357","span":{"begin":430,"end":433},"obj":"Protein"},{"id":"T14356","span":{"begin":161,"end":165},"obj":"Protein"},{"id":"T14355","span":{"begin":105,"end":110},"obj":"Protein"},{"id":"T14354","span":{"begin":4,"end":8},"obj":"Protein"}],"relations":[{"id":"R7410","pred":"causeOf","subj":"T14355","obj":"T14394"},{"id":"R7411","pred":"themeOf","subj":"T14356","obj":"T14395"},{"id":"R7412","pred":"themeOf","subj":"T14360","obj":"T14396"},{"id":"R7413","pred":"themeOf","subj":"T14364","obj":"T14397"},{"id":"R7414","pred":"themeOf","subj":"T14368","obj":"T14399"},{"id":"R7421","pred":"themeOf","subj":"T14395","obj":"T14394"},{"id":"R7422","pred":"themeOf","subj":"T14397","obj":"T14398"}],"text":"The CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml."}

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CD28 costimulatory pathway is crucial for effective antigen-specific T-cell cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3-kinase [24]. To evaluate the responsiveness of RA CD4+ T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without diluted concentrations of IL-10 for 36 hours, and IFN-γ production was measured by ELISA. As shown in Fig. 1, IFN-γ production by activated normal CD4+ T cells was mostly inhibited at concentrations as low as 1 ng/ml IL-10. However, RA CD4+ T cells were able to produce significant amounts of IFN-γ in the presence of 1 ng/ml IL-10, and the maximal but not complete inhibition by IL-10 was obtained at 10–100 ng/ml."}

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