PMC:1064873 / 12903-13760 JSON TXT

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LappsTest

{"project":"LappsTest","denotations":[{"id":"T10603","span":{"begin":422,"end":425},"obj":"Protein"},{"id":"T10602","span":{"begin":373,"end":376},"obj":"Protein"},{"id":"T10601","span":{"begin":342,"end":371},"obj":"Protein"},{"id":"T10600","span":{"begin":227,"end":231},"obj":"Protein"},{"id":"T10599","span":{"begin":198,"end":202},"obj":"Protein"},{"id":"T10598","span":{"begin":190,"end":194},"obj":"Protein"},{"id":"T10597","span":{"begin":85,"end":89},"obj":"Protein"},{"id":"T10596","span":{"begin":75,"end":80},"obj":"Protein"},{"id":"T10595","span":{"begin":0,"end":4},"obj":"Protein"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

biosemtest

pmc-enju-pas

bionlp-st-ge-2016-test-proteins

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T10969","span":{"begin":347,"end":351},"obj":"Protein"},{"id":"T10968","span":{"begin":227,"end":230},"obj":"Protein"},{"id":"T10967","span":{"begin":190,"end":194},"obj":"Protein"},{"id":"T10966","span":{"begin":85,"end":89},"obj":"Protein"},{"id":"T10965","span":{"begin":75,"end":80},"obj":"Protein"},{"id":"T10964","span":{"begin":0,"end":3},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T11063","span":{"begin":347,"end":351},"obj":"http://www.uniprot.org/uniprot/P05231"},{"id":"T11062","span":{"begin":190,"end":194},"obj":"http://www.uniprot.org/uniprot/P05231"},{"id":"T11061","span":{"begin":85,"end":89},"obj":"http://www.uniprot.org/uniprot/P05231"},{"id":"T11060","span":{"begin":75,"end":80},"obj":"http://www.uniprot.org/uniprot/P22301"},{"id":"T11059","span":{"begin":227,"end":230},"obj":"http://www.uniprot.org/uniprot/P01730"},{"id":"T11058","span":{"begin":0,"end":3},"obj":"http://www.uniprot.org/uniprot/P01730"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T10650","span":{"begin":809,"end":813},"obj":"http://purl.obolibrary.org/obo/UBERON_0001913"},{"id":"T10649","span":{"begin":286,"end":291},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"T10648","span":{"begin":184,"end":189},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T10937","span":{"begin":203,"end":218},"obj":"http://purl.obolibrary.org/obo/GO_0016310"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T11008","span":{"begin":775,"end":783},"obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"T11007","span":{"begin":724,"end":733},"obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"T11001","span":{"begin":363,"end":371},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T10995","span":{"begin":363,"end":371},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T10993","span":{"begin":480,"end":485},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T10992","span":{"begin":234,"end":239},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T10991","span":{"begin":114,"end":119},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T10990","span":{"begin":7,"end":12},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T10983","span":{"begin":363,"end":371},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T10982","span":{"begin":347,"end":351},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T10981","span":{"begin":190,"end":194},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T10980","span":{"begin":85,"end":89},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T10979","span":{"begin":75,"end":80},"obj":"http://purl.obolibrary.org/obo/GO_0005141"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

sentences

{"project":"sentences","denotations":[{"id":"T10931","span":{"begin":609,"end":857},"obj":"Sentence"},{"id":"T10930","span":{"begin":436,"end":608},"obj":"Sentence"},{"id":"T10929","span":{"begin":159,"end":435},"obj":"Sentence"},{"id":"T10928","span":{"begin":0,"end":158},"obj":"Sentence"},{"id":"T78","span":{"begin":0,"end":158},"obj":"Sentence"},{"id":"T79","span":{"begin":159,"end":435},"obj":"Sentence"},{"id":"T80","span":{"begin":436,"end":608},"obj":"Sentence"},{"id":"T81","span":{"begin":609,"end":857},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

simple1

{"project":"simple1","denotations":[{"id":"T11073","span":{"begin":347,"end":351},"obj":"Protein"},{"id":"T11072","span":{"begin":227,"end":230},"obj":"Protein"},{"id":"T11071","span":{"begin":190,"end":194},"obj":"Protein"},{"id":"T11070","span":{"begin":85,"end":89},"obj":"Protein"},{"id":"T11069","span":{"begin":75,"end":80},"obj":"Protein"},{"id":"T11068","span":{"begin":0,"end":3},"obj":"Protein"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

BioNLP16_DUT

{"project":"BioNLP16_DUT","denotations":[{"id":"T11898","span":{"begin":347,"end":351},"obj":"Protein"},{"id":"T11897","span":{"begin":227,"end":230},"obj":"Protein"},{"id":"T11896","span":{"begin":190,"end":194},"obj":"Protein"},{"id":"T11895","span":{"begin":85,"end":89},"obj":"Protein"},{"id":"T11894","span":{"begin":75,"end":80},"obj":"Protein"},{"id":"T11893","span":{"begin":0,"end":3},"obj":"Protein"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

BioNLP16_Messiy

{"project":"BioNLP16_Messiy","denotations":[{"id":"T11410","span":{"begin":347,"end":351},"obj":"Protein"},{"id":"T11409","span":{"begin":227,"end":230},"obj":"Protein"},{"id":"T11408","span":{"begin":190,"end":194},"obj":"Protein"},{"id":"T11407","span":{"begin":85,"end":89},"obj":"Protein"},{"id":"T11406","span":{"begin":75,"end":80},"obj":"Protein"},{"id":"T11405","span":{"begin":0,"end":3},"obj":"Protein"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

DLUT931

{"project":"DLUT931","denotations":[{"id":"T11764","span":{"begin":347,"end":351},"obj":"Protein"},{"id":"T11763","span":{"begin":227,"end":230},"obj":"Protein"},{"id":"T11762","span":{"begin":190,"end":194},"obj":"Protein"},{"id":"T11761","span":{"begin":85,"end":89},"obj":"Protein"},{"id":"T11760","span":{"begin":75,"end":80},"obj":"Protein"},{"id":"T11759","span":{"begin":0,"end":3},"obj":"Protein"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

bionlp-st-ge-2016-test-ihmc

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T11855","span":{"begin":75,"end":80},"obj":"Protein"},{"id":"T11853","span":{"begin":489,"end":499},"obj":"Protein"},{"id":"T11848","span":{"begin":661,"end":664},"obj":"Protein"},{"id":"T11847","span":{"begin":545,"end":563},"obj":"Protein"},{"id":"T11846","span":{"begin":657,"end":684},"obj":"Entity"},{"id":"T11844","span":{"begin":523,"end":531},"obj":"Entity"},{"id":"T11843","span":{"begin":817,"end":837},"obj":"Entity"},{"id":"T11841","span":{"begin":665,"end":679},"obj":"Entity"},{"id":"T11839","span":{"begin":542,"end":607},"obj":"Entity"},{"id":"T11838","span":{"begin":198,"end":202},"obj":"Protein"},{"id":"T11837","span":{"begin":352,"end":371},"obj":"Protein"},{"id":"T11836","span":{"begin":103,"end":119},"obj":"Entity"},{"id":"T11834","span":{"begin":283,"end":285},"obj":"Protein"},{"id":"T11833","span":{"begin":735,"end":764},"obj":"Protein"},{"id":"T11832","span":{"begin":528,"end":531},"obj":"Entity"},{"id":"T11830","span":{"begin":550,"end":562},"obj":"Entity"},{"id":"T11827","span":{"begin":480,"end":485},"obj":"Entity"},{"id":"T11889","span":{"begin":195,"end":218},"obj":"Phosphorylation"},{"id":"T11888","span":{"begin":159,"end":219},"obj":"Regulation"},{"id":"T11883","span":{"begin":500,"end":506},"obj":"Protein"},{"id":"T11880","span":{"begin":523,"end":531},"obj":"Entity"},{"id":"T11879","span":{"begin":533,"end":537},"obj":"Entity"},{"id":"T11878","span":{"begin":0,"end":12},"obj":"Entity"},{"id":"T11877","span":{"begin":771,"end":783},"obj":"Entity"},{"id":"T11876","span":{"begin":709,"end":723},"obj":"Entity"},{"id":"T11875","span":{"begin":85,"end":89},"obj":"Protein"},{"id":"T11873","span":{"begin":817,"end":830},"obj":"Entity"},{"id":"T11870","span":{"begin":220,"end":239},"obj":"Entity"},{"id":"T11869","span":{"begin":620,"end":635},"obj":"Protein"},{"id":"T11866","span":{"begin":181,"end":194},"obj":"Protein"},{"id":"T11862","span":{"begin":709,"end":765},"obj":"Entity"}],"relations":[{"id":"R6195","pred":"themeOf","subj":"T11838","obj":"T11889"},{"id":"R6197","pred":"causeOf","subj":"T11866","obj":"T11888"},{"id":"R6203","pred":"themeOf","subj":"T11889","obj":"T11888"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T11039","span":{"begin":174,"end":180},"obj":"Positive_regulation"},{"id":"T11038","span":{"begin":324,"end":336},"obj":"Negative_regulation"},{"id":"T11037","span":{"begin":324,"end":336},"obj":"Negative_regulation"},{"id":"T11036","span":{"begin":324,"end":336},"obj":"Negative_regulation"},{"id":"T11035","span":{"begin":203,"end":218},"obj":"Phosphorylation"},{"id":"T11034","span":{"begin":422,"end":425},"obj":"Protein"},{"id":"T11033","span":{"begin":373,"end":376},"obj":"Protein"},{"id":"T11032","span":{"begin":337,"end":371},"obj":"Protein"},{"id":"T11031","span":{"begin":227,"end":231},"obj":"Protein"},{"id":"T11030","span":{"begin":198,"end":202},"obj":"Protein"},{"id":"T11029","span":{"begin":184,"end":194},"obj":"Protein"},{"id":"T11028","span":{"begin":85,"end":89},"obj":"Protein"},{"id":"T11027","span":{"begin":75,"end":80},"obj":"Protein"},{"id":"T11026","span":{"begin":0,"end":4},"obj":"Protein"}],"relations":[{"id":"R5485","pred":"themeOf","subj":"T11030","obj":"T11035"},{"id":"R5486","pred":"themeOf","subj":"T11032","obj":"T11036"},{"id":"R5487","pred":"themeOf","subj":"T11033","obj":"T11037"},{"id":"R5488","pred":"themeOf","subj":"T11034","obj":"T11038"},{"id":"R5489","pred":"themeOf","subj":"T11035","obj":"T11039"},{"id":"R5484","pred":"causeOf","subj":"T11029","obj":"T11039"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

test3

{"project":"test3","denotations":[{"id":"T10635","span":{"begin":347,"end":351},"obj":"Protein"},{"id":"T10634","span":{"begin":227,"end":230},"obj":"Protein"},{"id":"T10633","span":{"begin":203,"end":218},"obj":"Phosphorylation"},{"id":"T10632","span":{"begin":190,"end":194},"obj":"Protein"},{"id":"T10631","span":{"begin":174,"end":180},"obj":"Regulation"},{"id":"T10630","span":{"begin":85,"end":89},"obj":"Protein"},{"id":"T10629","span":{"begin":75,"end":80},"obj":"Protein"},{"id":"T10628","span":{"begin":0,"end":3},"obj":"Protein"},{"id":"T10618","span":{"begin":347,"end":351},"obj":"Protein"},{"id":"T10617","span":{"begin":227,"end":230},"obj":"Protein"},{"id":"T10616","span":{"begin":190,"end":194},"obj":"Protein"},{"id":"T10615","span":{"begin":85,"end":89},"obj":"Protein"},{"id":"T10614","span":{"begin":75,"end":80},"obj":"Protein"},{"id":"T10613","span":{"begin":0,"end":3},"obj":"Protein"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

testone

{"project":"testone","denotations":[{"id":"T10592","span":{"begin":203,"end":218},"obj":"Phosphorylation"},{"id":"T10591","span":{"begin":174,"end":180},"obj":"Regulation"},{"id":"T10581","span":{"begin":347,"end":351},"obj":"Protein"},{"id":"T10580","span":{"begin":227,"end":230},"obj":"Protein"},{"id":"T10579","span":{"begin":190,"end":194},"obj":"Protein"},{"id":"T10578","span":{"begin":85,"end":89},"obj":"Protein"},{"id":"T10577","span":{"begin":75,"end":80},"obj":"Protein"},{"id":"T10576","span":{"begin":0,"end":3},"obj":"Protein"}],"relations":[{"id":"R5465","pred":"themeOf","subj":"T10579","obj":"T10592"},{"id":"R5469","pred":"themeOf","subj":"T10592","obj":"T10591"}],"text":"CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS. To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne). Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue). Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20."}

PMC:1064873 / 12903-13760 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:1064873 / 12903-13760 JSON TXT