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PMC:1064873 / 12881-14709 JSONTXT

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LappsTest

Id Subject Object Predicate Lexical cue
T10612 1805-1810 Protein denotes STAT1
T10611 1727-1739 Protein denotes STAT protein
T10610 1668-1672 Protein denotes STAT
T10609 1438-1462 Protein denotes anti-rabbit IgG antibody
T10608 1264-1278 Protein denotes STAT3 antibody
T10607 1187-1222 Protein denotes STAT1 antibody, anti-STAT3 antibody
T10606 1118-1149 Protein denotes rabbit IgG) anti-STAT1 antibody
T10605 918-923 Protein denotes STAT3
T10604 908-913 Protein denotes STAT1
T10603 444-447 Protein denotes IgG
T10602 395-398 Protein denotes IgG
T10601 364-393 Protein denotes anti-IL-6 polyclonal antibody
T10600 249-253 Protein denotes CD4+
T10599 220-224 Protein denotes STAT
T10598 212-216 Protein denotes IL-6
T10597 107-111 Protein denotes IL-6
T10596 97-102 Protein denotes IL-10
T10595 22-26 Protein denotes CD4+

biosemtest

Id Subject Object Predicate Lexical cue
T10710 1812-1817 UP/P42229 denotes hosop
T10859 1812-1817 UP/P42224 denotes hosop
T10926 708-712 UMLS/C0972569 denotes nd w
T10925 433-440 UMLS/C2587213 denotes ntrol g
T10924 1765-1775 UMLS/C0018873 denotes lls were u
T10923 1791-1807 UMLS/C1883676 denotes e control for ST
T10922 656-663 UMLS/C2347026 denotes s were
T10921 970-974 UMLS/C1704742 denotes Cell
T10920 970-974 UMLS/C0812225 denotes Cell
T10919 1053-1065 UMLS/C1442085 denotes ctions. Brie
T10918 1038-1052 UMLS/C1546933 denotes cturer's instr
T10917 555-558 UMLS/C0020259 denotes pH
T10916 1606-1611 UMLS/C0175723 denotes were
T10915 1460-1468 UMLS/C0003241 denotes dy dilut
T10914 1377-1385 UMLS/C0003241 denotes dy bindi
T10913 1276-1284 UMLS/C0003241 denotes dy, dilu
T10912 1220-1228 UMLS/C0003241 denotes dy, and
T10911 1199-1207 UMLS/C0003241 denotes dy, anti
T10910 1147-1155 UMLS/C0003241 denotes dy, anti
T10909 1112-1122 UMLS/C0003241 denotes dies (rabb
T10908 243-249 UMLS/C0205307 denotes ormal
T10907 1723-1729 UMLS/C1265611 denotes of ST
T10906 656-663 UMLS/C0870078 denotes s were
T10905 1717-1722 UMLS/C0439810 denotes amoun
T10904 581-589 UMLS/C0017861 denotes rol, 50
T10903 496-503 UMLS/C1704765 denotes acing c
T10902 309-314 UMLS/C1151254 denotes erum
T10901 207-212 UMLS/C1151254 denotes erum
T10900 318-332 UMLS/C0010454 denotes ulture medium
T10899 153-167 UMLS/C0010454 denotes ulture medium
T10898 1540-1543 UP/P02769 denotes nd
T10897 1372-1375 UP/P02769 denotes nti
T10896 504-509 UMLS/C0007634 denotes lls i
T10895 137-142 UMLS/C0007634 denotes ells
T10894 286-289 UMLS/C0439232 denotes in
T10893 58-61 UMLS/C0439232 denotes min
T10892 433-440 UMLS/C0332298 denotes ntrol g
T10891 1038-1052 UMLS/C1705426 denotes cturer's instr
T10890 572-575 UMLS/C1426244 denotes 10%
T10889 521-524 UMLS/C1426244 denotes ly
T10888 1386-1393 UMLS/C0243122 denotes g was d
T10887 441-445 UMLS/C1265549 denotes at I
T10886 361-365 UMLS/C1265549 denotes at a
T10885 1733-1745 UMLS/C1366753 denotes rotein. IFN-
T10884 1674-1678 UMLS/C1366753 denotes hosp
T10883 221-225 UMLS/C1366753 denotes TAT
T10882 1398-1406 UMLS/C0442726 denotes ed by ho
T10881 934-942 UMLS/C0442726 denotes ed using
T10805 97-102 UMLS/C1149215 denotes IL-10
T10804 1053-1065 UMLS/C0039401 denotes ctions. Brie
T10803 466-478 UMLS/C1881488 denotes ll lysates w
T10802 309-314 UMLS/C1546774 denotes erum
T10801 207-212 UMLS/C1546774 denotes erum
T10800 560-562 UMLS/C1304686 denotes 8]
T10799 1523-1531 UMLS/C0041415 denotes 20 with
T10798 1355-1363 UMLS/C0041415 denotes 20 with
T10797 876-884 UMLS/C0041415 denotes 20. Tyro
T10796 572-575 UMLS/C0272170 denotes 10%
T10795 521-524 UMLS/C0272170 denotes ly
T10794 286-289 UMLS/C1552937 denotes in
T10793 58-61 UMLS/C1552937 denotes min
T10792 213-217 UMLS/C1149241 denotes L-6
T10791 107-111 UMLS/C1149241 denotes IL-6
T10790 1812-1817 UMLS/C1335872 denotes hosop
T10789 1193-1198 UMLS/C1335872 denotes antib
T10788 914-919 UMLS/C1335872 denotes and S
T10787 504-509 UMLS/C1136359 denotes lls i
T10786 137-142 UMLS/C1136359 denotes ells
T10785 197-203 UMLS/C2348382 denotes ffect
T10784 1631-1643 UMLS/C0011321 denotes ometry using
T10783 1297-1308 UMLS/C0034866 denotes ended at 1/
T10782 1433-1443 UMLS/C0301869 denotes ated anti-
T10781 832-841 UMLS/C0349375 denotes ilk in Tr
T10780 117-124 UMLS/C0178587 denotes density
T10779 722-733 UMLS/C1547239 denotes erred to ni
T10778 441-445 UMLS/C1510458 denotes at I
T10777 361-365 UMLS/C1510458 denotes at a
T10776 504-509 UMLS/C1948049 denotes lls i
T10775 137-142 UMLS/C1948049 denotes ells
T10774 1398-1406 UMLS/C1511790 denotes ed by ho
T10773 934-942 UMLS/C1511790 denotes ed using
T10772 197-203 UMLS/C1280500 denotes ffect
T10771 213-217 UMLS/C1527132 denotes L-6
T10770 107-111 UMLS/C1527132 denotes IL-6
T10769 286-289 UMLS/C0702093 denotes in
T10768 58-61 UMLS/C0702093 denotes min
T10767 1540-1543 UMLS/C0005902 denotes nd
T10766 1372-1375 UMLS/C0005902 denotes nti
T10765 1053-1065 UMLS/C0220931 denotes ctions. Brie
T10764 1053-1065 UMLS/C1292796 denotes ctions. Brie
T10763 1469-1476 UMLS/C1948037 denotes d at 1/
T10762 1286-1293 UMLS/C1948037 denotes d as re
T10761 960-969 UMLS/C0470187 denotes ble kits
T10760 708-712 UMLS/C1382104 denotes nd w
T10759 656-663 UMLS/C0370003 denotes s were
T10758 560-562 UMLS/C0031526 denotes 8]
T10757 1674-1678 UMLS/C0205548 denotes hosp
T10756 221-225 UMLS/C0205548 denotes TAT
T10755 560-562 UMLS/C2363150 denotes 8]
T10754 722-733 UMLS/C0348011 denotes erred to ni
T10753 1753-1764 UMLS/C1948023 denotes ated Hela c
T10752 268-278 UMLS/C1948023 denotes timulated
T10751 40-50 UMLS/C1948023 denotes stimulated
T10750 97-102 UMLS/C1522550 denotes IL-10
T10749 560-562 UMLS/C0450409 denotes 8]
T10748 97-102 UMLS/C1334098 denotes IL-10
T10747 560-562 UMLS/C0450408 denotes 8]
T10746 560-562 UMLS/C0450407 denotes 8]
T10745 433-440 UMLS/C0009932 denotes ntrol g
T10744 722-733 UMLS/C1555583 denotes erred to ni
T10743 1540-1543 UMLS/C0036774 denotes nd
T10742 1372-1375 UMLS/C0036774 denotes nti
T10741 0-21 UMLS/C0949466 denotes Western blot analysis
T10740 213-217 UMLS/C0021760 denotes L-6
T10739 107-111 UMLS/C0021760 denotes IL-6
T10738 949-959 UMLS/C0680536 denotes cial avail
T10737 737-751 UMLS/C0600190 denotes ellulose membr
T10736 213-217 UMLS/C1334122 denotes L-6
T10735 107-111 UMLS/C1334122 denotes IL-6
T10734 1674-1678 UMLS/C1335960 denotes hosp
T10733 221-225 UMLS/C1335960 denotes TAT
T10732 991-1001 UMLS/C0039421 denotes logy, Beve
T10731 1540-1543 UMLS/C1511233 denotes nd
T10730 1372-1375 UMLS/C1511233 denotes nti
T10729 1433-1443 UMLS/C0522529 denotes ated anti-
T10728 722-733 UMLS/C1540850 denotes erred to ni
T10727 1674-1678 UMLS/C1420304 denotes hosp
T10726 221-225 UMLS/C1420304 denotes TAT
T10725 504-509 UMLS/C1704653 denotes lls i
T10724 137-142 UMLS/C1704653 denotes ells
T10723 722-733 UMLS/C0040671 denotes erred to ni
T10722 816-823 UMLS/C0332206 denotes d with
T10721 618-633 UMLS/C0006247 denotes henol blue). Th
T10720 708-712 UMLS/C0017243 denotes nd w
T10719 1460-1468 UMLS/C0021027 denotes dy dilut
T10718 1377-1385 UMLS/C0021027 denotes dy bindi
T10717 1276-1284 UMLS/C0021027 denotes dy, dilu
T10716 1220-1228 UMLS/C0021027 denotes dy, and
T10715 1199-1207 UMLS/C0021027 denotes dy, anti
T10714 1147-1155 UMLS/C0021027 denotes dy, anti
T10713 1112-1122 UMLS/C0021027 denotes dies (rabb
T10712 816-823 UMLS/C1706084 denotes d with
T10711 1124-1130 UMLS/C1446777 denotes IgG)
T10709 1193-1198 UP/P42229 denotes antib
T10708 914-919 UP/P42229 denotes and S
T10707 299-305 UMLS/C1561511 denotes ctive
T10706 299-305 UMLS/C1561512 denotes ctive
T10705 299-305 UMLS/C1561513 denotes ctive
T10704 69-78 UMLS/C1444656 denotes indicated
T10703 1410-1432 UMLS/C0019941 denotes adish peroxidase-conju
T10702 1598-1605 UMLS/C0033684 denotes n bands
T10701 648-655 UMLS/C0033684 denotes n sampl
T10700 722-733 UMLS/C1299988 denotes erred to ni
T10699 1723-1729 UMLS/C1561574 denotes of ST
T10698 1386-1393 UMLS/C1167622 denotes g was d
T10697 572-575 UMLS/C0037506 denotes 10%
T10696 521-524 UMLS/C0037506 denotes ly
T10695 1016-1019 UMLS/C0041703 denotes cco
T10694 425-428 UMLS/C0041703 denotes A)
T10693 1660-1668 UMLS/C1524024 denotes is, and
T10692 816-823 UMLS/C1533157 denotes d with
T10691 597-611 UMLS/C0012789 denotes othreitol, 0.1
T10690 299-305 UMLS/C0205177 denotes ctive
T10689 504-509 UMLS/C1269647 denotes lls i
T10688 137-142 UMLS/C1269647 denotes ells
T10687 1254-1262 UMLS/C1880236 denotes ne 705 o
T10686 1177-1185 UMLS/C1880236 denotes ne 701 o
T10685 460-465 UMLS/C0444667 denotes ole c
T10684 299-305 UMLS/C2347179 denotes ctive
T10683 1572-1589 UMLS/C1450278 denotes uminescence syste
T10682 97-102 UMLS/C0085295 denotes IL-10
T10681 243-249 UMLS/C1704701 denotes ormal
T10680 1031-1033 UMLS/C0319022 denotes m
T10679 734-736 UMLS/C0319022 denotes ro
T10678 182-184 UMLS/C0319022 denotes o
T10677 1469-1476 UMLS/C1720119 denotes d at 1/
T10676 1286-1293 UMLS/C1720119 denotes d as re
T10675 185-192 UMLS/C0332128 denotes xamine
T10674 816-823 UMLS/C0028778 denotes d with
T10673 243-249 UMLS/C1550457 denotes ormal
T10672 816-823 UMLS/C0233660 denotes d with
T10671 299-305 UMLS/C1561510 denotes ctive
T10670 299-305 UMLS/C1561509 denotes ctive
T10669 299-305 UMLS/C1561508 denotes ctive
T10668 299-305 UMLS/C1561507 denotes ctive
T10667 1456-1459 UMLS/C0020852 denotes tib
T10666 1131-1134 UMLS/C0020852 denotes nti
T10665 446-449 UMLS/C0020852 denotes G (
T10664 397-400 UMLS/C0020852 denotes G;
T10663 496-503 UMLS/C1882509 denotes acing c
T10662 1679-1694 UMLS/C0031715 denotes orylation was c
T10661 226-241 UMLS/C0031715 denotes hosphorylation,
T10660 1386-1393 UMLS/C1145667 denotes g was d
T10659 243-249 UMLS/C1551394 denotes ormal
T10658 1124-1130 UMLS/C0034493 denotes IgG)
T10657 1386-1393 UMLS/C2349209 denotes g was d
T10656 299-305 UMLS/C1547419 denotes ctive
T10655 1254-1262 UMLS/C0041485 denotes ne 705 o
T10654 1177-1185 UMLS/C0041485 denotes ne 701 o
T10653 970-974 UMLS/C1705212 denotes Cell
T10652 708-712 UMLS/C1551386 denotes nd w
T10651 433-440 UMLS/C1882979 denotes ntrol g
T10880 376-395 UMLS/C0312586 denotes lyclonal antibody (
T10879 1553-1561 UMLS/C0443289 denotes ed using
T10878 243-249 UMLS/C2347086 denotes ormal
T10877 79-93 UMLS/C0086045 denotes concentrations
T10876 1660-1668 UMLS/C0936012 denotes is, and
T10875 1021-1030 UMLS/C0680240 denotes ing to th
T10874 441-445 UMLS/C0018019 denotes at I
T10873 361-365 UMLS/C0018019 denotes at a
T10872 484-492 UMLS/C1521827 denotes epared b
T10871 1038-1052 UMLS/C0947322 denotes cturer's instr
T10870 572-575 UMLS/C1419917 denotes 10%
T10869 521-524 UMLS/C1419917 denotes ly
T10868 1717-1722 UMLS/C0439175 denotes amoun
T10867 1270-1275 UMLS/C1367307 denotes antib
T10866 924-929 UMLS/C1367307 denotes was d
T10865 243-249 UMLS/C0439166 denotes ormal
T10864 532-538 UMLS/C0006353 denotes fer (6
T10863 1540-1543 UMLS/C0487992 denotes nd
T10862 1372-1375 UMLS/C0487992 denotes nti
T10861 433-440 UMLS/C0243148 denotes ntrol g
T10860 581-589 UMLS/C0768477 denotes rol, 50
T10858 1193-1198 UP/P42224 denotes antib
T10857 914-919 UP/P42224 denotes and S
T10856 1572-1589 UMLS/C0162524 denotes uminescence syste
T10855 1606-1611 UMLS/C0439645 denotes were
T10854 1506-1512 UMLS/C0036082 denotes with
T10853 1338-1344 UMLS/C0036082 denotes with
T10852 859-865 UMLS/C0036082 denotes with
T10851 1038-1052 UMLS/C1548599 denotes cturer's instr
T10850 970-974 UMLS/C1690540 denotes Cell
T10849 886-910 UMLS/C1519726 denotes ne phosphorylation of ST
T10848 309-314 UMLS/C1550100 denotes erum
T10847 207-212 UMLS/C1550100 denotes erum
T10846 976-990 UMLS/C0037083 denotes ignaling Techn
T10845 1590-1596 UMLS/C1553451 denotes . Prot
T10844 970-974 UMLS/C1553450 denotes Cell
T10843 722-733 UMLS/C1705822 denotes erred to ni
T10842 1093-1102 UMLS/C1439852 denotes ted with
T10841 1590-1596 UMLS/C0449913 denotes . Prot
T10840 433-440 UMLS/C1547100 denotes ntrol g
T10839 1270-1275 UMLS/C0253050 denotes antib
T10838 924-929 UMLS/C0253050 denotes was d
T10837 1386-1393 UMLS/C1689898 denotes g was d
T10836 243-249 UMLS/C1873497 denotes ormal
T10835 1053-1065 UMLS/C0302828 denotes ctions. Brie
T10834 773-788 UMLS/C0454842 denotes ghamshire, UK),
T10833 1590-1596 UMLS/C1704459 denotes . Prot
T10832 1460-1468 UMLS/C1621287 denotes dy dilut
T10831 1377-1385 UMLS/C1621287 denotes dy bindi
T10830 1276-1284 UMLS/C1621287 denotes dy, dilu
T10829 1220-1228 UMLS/C1621287 denotes dy, and
T10828 1199-1207 UMLS/C1621287 denotes dy, anti
T10827 1147-1155 UMLS/C1621287 denotes dy, anti
T10826 1112-1122 UMLS/C1621287 denotes dies (rabb
T10825 1456-1459 UMLS/C0202087 denotes tib
T10824 1131-1134 UMLS/C0202087 denotes nti
T10823 446-449 UMLS/C0202087 denotes G (
T10822 397-400 UMLS/C0202087 denotes G;
T10821 299-305 UMLS/C1553875 denotes ctive
T10820 255-262 UMLS/C0039194 denotes cells
T10819 27-34 UMLS/C0039194 denotes T cells
T10818 656-663 UMLS/C0441621 denotes s were
T10817 560-562 UMLS/C0020283 denotes 8]
T10816 1699-1707 UMLS/C1707455 denotes ed with
T10815 433-440 UMLS/C1550141 denotes ntrol g
T10814 1606-1611 UMLS/C0185014 denotes were
T10813 309-314 UMLS/C0229671 denotes erum
T10812 207-212 UMLS/C0229671 denotes erum
T10811 722-733 UMLS/C0728827 denotes erred to ni
T10810 79-93 UMLS/C1446561 denotes concentrations
T10809 1674-1678 UMLS/C1548425 denotes hosp
T10808 221-225 UMLS/C1548425 denotes TAT
T10807 1660-1668 UMLS/C0002778 denotes is, and
T10806 669-681 UMLS/C1979893 denotes onated on 10

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T11404 1811-1827 NN denotes phosophorylation
T11403 1805-1810 NN denotes STAT1
T11402 1801-1804 IN denotes for
T11401 1793-1800 NN denotes control
T11400 1784-1792 JJ denotes positive
T11399 1782-1783 DT denotes a
T11398 1779-1781 IN denotes as
T11397 1774-1778 VB denotes used
T11396 1769-1773 VB denotes were
T11395 1763-1768 NN denotes cells
T11394 1758-1762 NN denotes Hela
T11393 1741-1757 JJ denotes IFN-γ-stimulated
T11392 1732-1739 NN denotes protein
T11391 1727-1731 NN denotes STAT
T11390 1724-1726 IN denotes of
T11389 1717-1723 NN denotes amount
T11315 1248-1256 NN denotes tyrosine
T11314 1228-1247 JJ denotes anti-phosphorylated
T11313 1224-1227 CC denotes and
T11312 1222-1223 -COMMA- denotes ,
T11311 1214-1222 NN denotes antibody
T11310 1203-1213 JJ denotes anti-STAT3
T11309 1201-1202 -COMMA- denotes ,
T11308 1193-1201 NN denotes antibody
T11307 1187-1192 NN denotes STAT1
T11306 1184-1186 IN denotes of
T11305 1180-1183 CD denotes 701
T11304 1171-1179 NN denotes tyrosine
T11303 1151-1170 JJ denotes anti-phosphorylated
T11302 1149-1150 -COMMA- denotes ,
T11301 1141-1149 NN denotes antibody
T11300 1130-1140 JJ denotes anti-STAT1
T11299 1128-1129 -RRB- denotes )
T11298 1125-1128 NN denotes IgG
T11297 1118-1124 NN denotes rabbit
T11296 1117-1118 -LRB- denotes (
T11295 1106-1116 NN denotes antibodies
T11294 1102-1105 DT denotes the
T11293 1097-1101 IN denotes with
T11292 1087-1096 VB denotes incubated
T11291 1083-1086 VB denotes was
T11290 1074-1082 NN denotes membrane
T11289 1070-1073 DT denotes the
T11288 1068-1069 -COMMA- denotes ,
T11287 1061-1068 NNP denotes Briefly
T11286 1047-1059 NN denotes instructions
T11285 1044-1046 POS denotes 's
T11284 1032-1044 NN denotes manufacturer
T11283 1028-1031 DT denotes the
T11282 1025-1027 TO denotes to
T11281 1015-1024 VB denotes according
T11280 1013-1014 -RRB- denotes )
T11279 1010-1013 NNP denotes USA
T11278 1008-1009 -COMMA- denotes ,
T11277 1006-1008 NNP denotes MA
T11276 1004-1005 -COMMA- denotes ,
T11275 997-1004 NNP denotes Beverly
T11274 995-996 -COMMA- denotes ,
T11273 985-995 NNP denotes Technology
T11272 975-984 NNP denotes Signaling
T11271 970-974 NNP denotes Cell
T11270 969-970 -LRB- denotes (
T11269 964-968 NN denotes kits
T11268 954-963 JJ denotes available
T11267 943-953 JJ denotes commercial
T11266 937-942 VB denotes using
T11265 928-936 VB denotes detected
T11264 924-927 VB denotes was
T11263 918-923 NN denotes STAT3
T11262 914-917 CC denotes and
T11261 908-913 NN denotes STAT1
T11260 905-907 IN denotes of
T11259 889-904 NN denotes phosphorylation
T11258 880-888 NN denotes Tyrosine
T11257 876-878 CD denotes 20
T11256 870-875 CD denotes Tween
T11255 868-869 NN denotes %
T11254 865-868 CD denotes 0.1
T11253 860-864 IN denotes with
T11252 853-859 NN denotes saline
T11251 839-852 JJ denotes Tris-buffered
T11250 836-838 IN denotes in
T11249 831-835 NN denotes milk
T11248 826-830 NN denotes skim
T11247 824-825 NN denotes %
T11246 823-824 CD denotes 5
T11245 818-822 IN denotes with
T11244 810-817 VB denotes blocked
T11243 806-809 VB denotes was
T11242 797-805 NN denotes membrane
T11241 793-796 DT denotes the
T11240 789-792 CC denotes and
T11239 787-788 -COMMA- denotes ,
T11238 786-787 -RRB- denotes )
T11237 784-786 NNP denotes UK
T11236 782-783 -COMMA- denotes ,
T11235 767-782 NNP denotes Buckinghamshire
T11234 765-766 -COMMA- denotes ,
T11233 757-765 NNP denotes Amersham
T11232 756-757 -LRB- denotes (
T11231 746-755 NN denotes membranes
T11230 731-745 VB denotes nitrocellulose
T11229 728-730 TO denotes to
T11228 716-727 VB denotes transferred
T11227 711-715 VB denotes were
T11226 707-710 CC denotes and
T11225 702-706 NN denotes gels
T11224 683-701 NN denotes SDS-polyacrylamide
T11223 681-682 NN denotes %
T11222 679-681 CD denotes 10
T11221 676-678 IN denotes on
T11220 663-675 VB denotes fractionated
T11219 658-662 VB denotes were
T11218 650-657 NN denotes samples
T11217 642-649 NN denotes protein
T11216 639-641 NN denotes μl
T11215 636-638 CD denotes 20
T11214 631-635 RB denotes Then
T11213 628-629 -RRB- denotes )
T11212 624-628 NN denotes blue
T11211 613-623 NN denotes bromphenol
T11210 611-612 NN denotes %
T11209 608-611 CD denotes 0.1
T11208 606-607 -COMMA- denotes ,
T11207 592-606 NN denotes dithiothreitol
T11206 589-591 NN denotes mM
T11205 586-588 CD denotes 50
T11204 584-585 -COMMA- denotes ,
T11203 576-584 NN denotes glycerol
T11202 574-575 NN denotes %
T11201 572-574 CD denotes 10
T11200 570-571 -COMMA- denotes ,
T11199 567-570 NN denotes SDS
T11198 565-566 NN denotes %
T11197 564-565 CD denotes 2
T11196 562-563 -COMMA- denotes ,
T11195 561-562 -RRB- denotes ]
T11194 558-561 CD denotes 6.8
T11193 555-557 NN denotes pH
T11192 554-555 -LRB- denotes [
T11191 545-553 NN denotes Tris–HCl
T11190 542-544 NN denotes mM
T11189 537-541 CD denotes 62.5
T11188 536-537 -LRB- denotes (
T11187 529-535 NN denotes buffer
T11186 522-528 VB denotes lysing
T11185 518-521 NN denotes SDS
T11184 515-517 NN denotes μl
T11183 511-514 CD denotes 100
T11182 508-510 IN denotes in
T11181 502-507 NN denotes cells
T11180 494-501 VB denotes placing
T11179 491-493 IN denotes by
T11178 482-490 VB denotes prepared
T11177 477-481 VB denotes were
T11176 469-476 NN denotes lysates
T11175 464-468 NN denotes cell
T11174 458-463 JJ denotes Whole
T11173 455-456 -RRB- denotes )
T11172 449-455 NN denotes Techne
T11171 448-449 -LRB- denotes (
T11170 444-447 NN denotes IgG
T11169 439-443 NN denotes goat
T11168 431-438 NN denotes control
T11167 428-430 CC denotes or
T11166 426-427 -RRB- denotes )
T11165 423-426 NNP denotes USA
T11164 421-422 -COMMA- denotes ,
T11163 419-421 NNP denotes NJ
T11162 417-418 -COMMA- denotes ,
T11161 408-417 NNP denotes Princeton
T11160 406-407 -COMMA- denotes ,
T11159 400-406 NNP denotes Techne
T11158 398-399 -COLON- denotes ;
T11157 395-398 NN denotes IgG
T11156 394-395 -LRB- denotes (
T11155 385-393 NN denotes antibody
T11154 374-384 JJ denotes polyclonal
T11153 364-373 JJ denotes anti-IL-6
T11152 359-363 NN denotes goat
T11151 346-358 VB denotes neutralizing
T11150 340-345 NN denotes μg/ml
T11149 337-339 CD denotes 40
T11148 332-336 IN denotes with
T11147 325-331 NN denotes medium
T11146 317-324 NN denotes culture
T11145 314-316 IN denotes in
T11144 308-313 NN denotes serum
T11143 305-307 NN denotes RA
T11142 298-304 JJ denotes active
T11141 296-297 NN denotes %
T11140 294-296 CD denotes 30
T11139 289-293 IN denotes with
T11138 285-288 NN denotes min
T11137 282-284 CD denotes 20
T11136 278-281 IN denotes for
T11135 267-277 VB denotes stimulated
T11134 262-266 VB denotes were
T11133 256-261 NN denotes cells
T11132 254-255 NN denotes T
T11131 249-253 JJ denotes CD4+
T11130 242-248 JJ denotes normal
T11129 240-241 -COMMA- denotes ,
T11128 225-240 NN denotes phosphorylation
T11127 220-224 NN denotes STAT
T11126 217-219 IN denotes on
T11125 212-216 NN denotes IL-6
T11124 206-211 NN denotes serum
T11123 203-205 IN denotes of
T11122 196-202 NN denotes effect
T11121 192-195 DT denotes the
T11120 184-191 VB denotes examine
T11119 181-183 TO denotes To
T11118 176-179 NN denotes FCS
T11117 174-175 NN denotes %
T11116 172-174 CD denotes 10
T11115 167-171 IN denotes with
T11114 160-166 NN denotes medium
T11113 152-159 NN denotes culture
T11112 149-151 NN denotes ml
T11111 145-148 CD denotes 0.5
T11110 142-144 IN denotes in
T11109 136-141 NN denotes cells
T11108 132-135 CD denotes 105
T11107 130-131 IN denotes ×
T11106 128-129 CD denotes 5
T11105 125-127 IN denotes of
T11104 117-124 NN denotes density
T11103 115-116 DT denotes a
T11102 112-114 IN denotes at
T11101 107-111 NN denotes IL-6
T11100 103-106 CC denotes and
T11096 69-78 VB denotes indicated
T11095 65-68 DT denotes the
T11094 62-64 IN denotes by
T11093 58-61 NN denotes min
T11092 55-57 CD denotes 20
T11091 51-54 IN denotes for
T11090 40-50 VB denotes stimulated
T11089 35-39 VB denotes were
T11088 29-34 NN denotes cells
T11087 27-28 NN denotes T
T11086 22-26 JJ denotes CD4+
T11085 13-21 NN denotes analysis
T11084 8-12 NN denotes blot
T11083 0-7 NN denotes Western
T11388 1711-1716 JJ denotes total
T11387 1707-1710 DT denotes the
T11386 1702-1706 IN denotes with
T11385 1693-1701 VB denotes compared
T11384 1689-1692 VB denotes was
T11383 1673-1688 NN denotes phosphorylation
T11382 1668-1672 NN denotes STAT
T11381 1664-1667 CC denotes and
T11380 1662-1663 -COMMA- denotes ,
T11379 1654-1662 NN denotes analysis
T11378 1644-1653 NN denotes NIH-Image
T11377 1638-1643 VB denotes using
T11376 1625-1637 NN denotes densitometry
T11375 1622-1624 IN denotes by
T11374 1611-1621 VB denotes quantified
T11373 1606-1610 VB denotes were
T11372 1600-1605 NN denotes bands
T11371 1592-1599 NN denotes Protein
T11370 1584-1590 NN denotes system
T11369 1566-1583 NN denotes chemiluminescence
T11368 1562-1565 DT denotes the
T11367 1556-1561 VB denotes using
T11366 1547-1555 VB denotes revealed
T11365 1543-1546 VB denotes was
T11364 1539-1542 CC denotes and
T11363 1537-1538 -COMMA- denotes ,
T11362 1534-1537 NN denotes BSA
T11361 1532-1533 NN denotes %
T11360 1531-1532 CD denotes 5
T11359 1526-1530 IN denotes with
T11358 1523-1525 CD denotes 20
T11357 1517-1522 CD denotes Tween
T11356 1515-1516 NN denotes %
T11355 1512-1515 CD denotes 0.1
T11354 1507-1511 IN denotes with
T11353 1500-1506 NN denotes saline
T11352 1486-1499 JJ denotes Tris-buffered
T11351 1481-1485 IN denotes with
T11350 1474-1480 CD denotes 1/4000
T11349 1471-1473 IN denotes at
T11348 1463-1470 VB denotes diluted
T11347 1454-1462 NN denotes antibody
T11346 1450-1453 NN denotes IgG
T11345 1438-1449 JJ denotes anti-rabbit
T11344 1416-1437 JJ denotes peroxidase-conjugated
T11343 1404-1415 JJ denotes horseradish
T11342 1401-1403 IN denotes by
T11341 1392-1400 VB denotes detected
T11340 1388-1391 VB denotes was
T11339 1380-1387 NN denotes binding
T11338 1371-1379 NN denotes Antibody
T11337 1366-1369 NN denotes BSA
T11336 1364-1365 NN denotes %
T11335 1363-1364 CD denotes 5
T11334 1358-1362 IN denotes with
T11333 1355-1357 CD denotes 20
T11332 1349-1354 CD denotes Tween
T11331 1347-1348 NN denotes %
T11330 1344-1347 CD denotes 0.1
T11329 1339-1343 IN denotes with
T11328 1332-1338 NN denotes saline
T11327 1318-1331 JJ denotes Tris-buffered
T11326 1313-1317 IN denotes with
T11325 1306-1312 CD denotes 1/2000
T11324 1303-1305 IN denotes at
T11323 1291-1302 VB denotes recommended
T11322 1288-1290 IN denotes as
T11321 1280-1287 VB denotes diluted
T11320 1278-1279 -COMMA- denotes ,
T11319 1270-1278 NN denotes antibody
T11318 1264-1269 NN denotes STAT3
T11317 1261-1263 IN denotes of
T11316 1257-1260 CD denotes 705
T11099 97-102 NN denotes IL-10
T11098 94-96 IN denotes of
T11097 79-93 NN denotes concentrations
R5501 T11085 T11083 arg1Of analysis,Western
R5502 T11085 T11084 arg1Of analysis,blot
R5503 T11088 T11086 arg1Of cells,CD4+
R5504 T11088 T11087 arg1Of cells,T
R5505 T11088 T11089 arg1Of cells,were
R5506 T11088 T11090 arg2Of cells,stimulated
R5507 T11090 T11089 arg2Of stimulated,were
R5508 T11090 T11091 arg1Of stimulated,for
R5509 T11090 T11094 arg1Of stimulated,by
R5510 T11090 T11102 arg1Of stimulated,at
R5511 T11093 T11091 arg2Of min,for
R5512 T11093 T11092 arg1Of min,20
R5513 T11097 T11094 arg2Of concentrations,by
R5514 T11097 T11095 arg1Of concentrations,the
R5515 T11097 T11096 arg2Of concentrations,indicated
R5516 T11097 T11098 arg1Of concentrations,of
R5517 T11099 T11100 arg1Of IL-10,and
R5518 T11100 T11098 arg2Of and,of
R5519 T11101 T11100 arg2Of IL-6,and
R5520 T11104 T11102 arg2Of density,at
R5521 T11104 T11103 arg1Of density,a
R5522 T11104 T11105 arg1Of density,of
R5523 T11104 T11110 arg1Of density,in
R5524 T11108 T11107 arg1Of 105,×
R5525 T11109 T11105 arg2Of cells,of
R5526 T11109 T11106 arg1Of cells,5
R5527 T11109 T11108 arg1Of cells,105
R5528 T11111 T11112 arg1Of 0.5,ml
R5529 T11114 T11110 arg2Of medium,in
R5530 T11114 T11111 arg1Of medium,0.5
R5531 T11114 T11113 arg1Of medium,culture
R5532 T11114 T11115 arg1Of medium,with
R5533 T11116 T11117 arg1Of 10,%
R5534 T11118 T11115 arg2Of FCS,with
R5535 T11118 T11116 arg1Of FCS,10
R5536 T11120 T11119 arg1Of examine,To
R5537 T11122 T11120 arg2Of effect,examine
R5538 T11122 T11121 arg1Of effect,the
R5539 T11122 T11123 arg1Of effect,of
R5540 T11122 T11126 arg1Of effect,on
R5541 T11125 T11123 arg2Of IL-6,of
R5542 T11125 T11124 arg1Of IL-6,serum
R5543 T11128 T11126 arg2Of phosphorylation,on
R5544 T11128 T11127 arg1Of phosphorylation,STAT
R5545 T11133 T11130 arg1Of cells,normal
R5546 T11133 T11131 arg1Of cells,CD4+
R5547 T11133 T11132 arg1Of cells,T
R5548 T11133 T11134 arg1Of cells,were
R5549 T11133 T11135 arg2Of cells,stimulated
R5550 T11135 T11119 modOf stimulated,To
R5551 T11135 T11129 arg1Of stimulated,","
R5552 T11135 T11134 arg2Of stimulated,were
R5553 T11135 T11136 arg1Of stimulated,for
R5554 T11135 T11139 arg1Of stimulated,with
R5555 T11138 T11136 arg2Of min,for
R5556 T11138 T11137 arg1Of min,20
R5557 T11140 T11141 arg1Of 30,%
R5558 T11144 T11139 arg2Of serum,with
R5559 T11144 T11140 arg1Of serum,30
R5560 T11144 T11142 arg1Of serum,active
R5561 T11144 T11143 arg1Of serum,RA
R5562 T11144 T11145 arg1Of serum,in
R5563 T11144 T11148 arg1Of serum,with
R5564 T11147 T11145 arg2Of medium,in
R5565 T11147 T11146 arg1Of medium,culture
R5566 T11150 T11148 arg2Of μg/ml,with
R5567 T11150 T11149 arg1Of μg/ml,40
R5568 T11150 T11151 arg1Of μg/ml,neutralizing
R5569 T11155 T11152 arg1Of antibody,goat
R5570 T11155 T11153 arg1Of antibody,anti-IL-6
R5571 T11155 T11154 arg1Of antibody,polyclonal
R5572 T11155 T11156 arg1Of antibody,(
R5573 T11155 T11167 arg1Of antibody,or
R5574 T11157 T11156 arg2Of IgG,(
R5575 T11157 T11158 arg1Of IgG,;
R5576 T11157 T11164 arg1Of IgG,","
R5577 T11157 T11165 arg1Of IgG,USA
R5578 T11159 T11160 arg1Of Techne,","
R5579 T11160 T11162 arg1Of ",",","
R5580 T11161 T11160 arg2Of Princeton,","
R5581 T11162 T11158 arg2Of ",",;
R5582 T11163 T11162 arg2Of NJ,","
R5583 T11166 T11156 arg3Of ),(
R5584 T11167 T11151 arg2Of or,neutralizing
R5585 T11170 T11167 arg2Of IgG,or
R5586 T11170 T11168 arg1Of IgG,control
R5587 T11170 T11169 arg1Of IgG,goat
R5588 T11170 T11171 arg1Of IgG,(
R5589 T11172 T11171 arg2Of Techne,(
R5590 T11173 T11171 arg3Of ),(
R5591 T11176 T11174 arg1Of lysates,Whole
R5592 T11176 T11175 arg1Of lysates,cell
R5593 T11176 T11177 arg1Of lysates,were
R5594 T11176 T11178 arg2Of lysates,prepared
R5595 T11178 T11177 arg2Of prepared,were
R5596 T11178 T11179 arg1Of prepared,by
R5597 T11180 T11179 arg2Of placing,by
R5598 T11180 T11182 arg1Of placing,in
R5599 T11181 T11180 arg2Of cells,placing
R5600 T11185 T11182 arg2Of SDS,in
R5601 T11185 T11183 arg1Of SDS,100
R5602 T11185 T11184 arg1Of SDS,μl
R5603 T11185 T11186 arg1Of SDS,lysing
R5604 T11187 T11186 arg2Of buffer,lysing
R5605 T11187 T11188 arg1Of buffer,(
R5606 T11189 T11190 arg1Of 62.5,mM
R5607 T11191 T11189 arg1Of Tris–HCl,62.5
R5608 T11191 T11192 arg1Of Tris–HCl,[
R5609 T11191 T11196 arg1Of Tris–HCl,","
R5610 T11193 T11192 arg2Of pH,[
R5611 T11193 T11194 arg1Of pH,6.8
R5612 T11195 T11192 arg3Of ],[
R5613 T11196 T11188 arg2Of ",",(
R5614 T11197 T11198 arg1Of 2,%
R5615 T11199 T11197 arg1Of SDS,2
R5616 T11199 T11200 arg1Of SDS,","
R5617 T11200 T11204 arg1Of ",",","
R5618 T11201 T11202 arg1Of 10,%
R5619 T11203 T11200 arg2Of glycerol,","
R5620 T11203 T11201 arg1Of glycerol,10
R5621 T11204 T11208 arg1Of ",",","
R5622 T11205 T11206 arg1Of 50,mM
R5623 T11207 T11204 arg2Of dithiothreitol,","
R5624 T11207 T11205 arg1Of dithiothreitol,50
R5625 T11208 T11196 arg2Of ",",","
R5626 T11209 T11210 arg1Of 0.1,%
R5627 T11212 T11208 arg2Of blue,","
R5628 T11212 T11209 arg1Of blue,0.1
R5629 T11212 T11211 arg1Of blue,bromphenol
R5630 T11213 T11188 arg3Of ),(
R5631 T11215 T11214 arg1Of 20,Then
R5632 T11218 T11215 arg1Of samples,20
R5633 T11218 T11216 arg1Of samples,μl
R5634 T11218 T11217 arg1Of samples,protein
R5635 T11218 T11219 arg1Of samples,were
R5636 T11218 T11220 arg2Of samples,fractionated
R5637 T11218 T11227 arg1Of samples,were
R5638 T11218 T11228 arg2Of samples,transferred
R5639 T11220 T11219 arg2Of fractionated,were
R5640 T11220 T11221 arg1Of fractionated,on
R5641 T11220 T11226 arg1Of fractionated,and
R5642 T11222 T11223 arg1Of 10,%
R5643 T11225 T11221 arg2Of gels,on
R5644 T11225 T11222 arg1Of gels,10
R5645 T11225 T11224 arg1Of gels,SDS-polyacrylamide
R5646 T11226 T11232 arg1Of and,(
R5647 T11226 T11240 arg1Of and,and
R5648 T11228 T11226 arg2Of transferred,and
R5649 T11228 T11227 arg2Of transferred,were
R5650 T11228 T11229 modOf transferred,to
R5651 T11230 T11229 arg1Of nitrocellulose,to
R5652 T11231 T11230 arg2Of membranes,nitrocellulose
R5653 T11233 T11234 arg1Of Amersham,","
R5654 T11234 T11236 arg1Of ",",","
R5655 T11235 T11234 arg2Of Buckinghamshire,","
R5656 T11236 T11232 arg2Of ",",(
R5657 T11237 T11236 arg2Of UK,","
R5658 T11238 T11232 arg3Of ),(
R5659 T11240 T11239 arg1Of and,","
R5660 T11242 T11241 arg1Of membrane,the
R5661 T11242 T11243 arg1Of membrane,was
R5662 T11242 T11244 arg2Of membrane,blocked
R5663 T11244 T11240 arg2Of blocked,and
R5664 T11244 T11243 arg2Of blocked,was
R5665 T11244 T11245 arg1Of blocked,with
R5666 T11249 T11245 arg2Of milk,with
R5667 T11249 T11246 arg1Of milk,5
R5668 T11249 T11247 arg1Of milk,%
R5669 T11249 T11248 arg1Of milk,skim
R5670 T11249 T11250 arg1Of milk,in
R5671 T11252 T11250 arg2Of saline,in
R5672 T11252 T11251 arg1Of saline,Tris-buffered
R5673 T11252 T11253 arg1Of saline,with
R5674 T11255 T11254 arg1Of %,0.1
R5675 T11255 T11256 arg1Of %,Tween
R5676 T11257 T11253 arg2Of 20,with
R5677 T11257 T11255 arg1Of 20,%
R5678 T11259 T11258 arg1Of phosphorylation,Tyrosine
R5679 T11259 T11260 arg1Of phosphorylation,of
R5680 T11259 T11264 arg1Of phosphorylation,was
R5681 T11259 T11265 arg2Of phosphorylation,detected
R5682 T11259 T11266 arg1Of phosphorylation,using
R5683 T11261 T11262 arg1Of STAT1,and
R5684 T11262 T11260 arg2Of and,of
R5685 T11263 T11262 arg2Of STAT3,and
R5686 T11265 T11264 arg2Of detected,was
R5687 T11266 T11265 arg3Of using,detected
R5688 T11266 T11281 arg1Of using,according
R5689 T11269 T11266 arg2Of kits,using
R5690 T11269 T11267 arg1Of kits,commercial
R5691 T11269 T11268 arg1Of kits,available
R5692 T11269 T11270 arg1Of kits,(
R5693 T11273 T11270 arg2Of Technology,(
R5694 T11273 T11271 arg1Of Technology,Cell
R5695 T11273 T11272 arg1Of Technology,Signaling
R5696 T11273 T11274 arg1Of Technology,","
R5697 T11273 T11278 arg1Of Technology,","
R5698 T11273 T11279 arg1Of Technology,USA
R5699 T11275 T11276 arg1Of Beverly,","
R5700 T11276 T11274 arg2Of ",",","
R5701 T11277 T11276 arg2Of MA,","
R5702 T11280 T11270 arg3Of ),(
R5703 T11282 T11281 arg2Of to,according
R5704 T11284 T11283 arg1Of manufacturer,the
R5705 T11284 T11285 arg2Of manufacturer,'s
R5706 T11286 T11282 arg2Of instructions,to
R5707 T11286 T11285 arg1Of instructions,'s
R5708 T11290 T11289 arg1Of membrane,the
R5709 T11290 T11291 arg1Of membrane,was
R5710 T11290 T11292 arg2Of membrane,incubated
R5711 T11292 T11287 arg1Of incubated,Briefly
R5712 T11292 T11288 arg1Of incubated,","
R5713 T11292 T11291 arg2Of incubated,was
R5714 T11292 T11293 arg1Of incubated,with
R5715 T11295 T11296 arg1Of antibodies,(
R5716 T11298 T11296 arg2Of IgG,(
R5717 T11298 T11297 arg1Of IgG,rabbit
R5718 T11299 T11296 arg3Of ),(
R5719 T11301 T11293 arg2Of antibody,with
R5720 T11301 T11294 arg1Of antibody,the
R5721 T11301 T11295 arg1Of antibody,antibodies
R5722 T11301 T11300 arg1Of antibody,anti-STAT1
R5723 T11301 T11302 arg1Of antibody,","
R5724 T11304 T11302 arg2Of tyrosine,","
R5725 T11304 T11303 arg1Of tyrosine,anti-phosphorylated
R5726 T11304 T11305 arg1Of tyrosine,701
R5727 T11304 T11306 arg1Of tyrosine,of
R5728 T11304 T11320 arg1Of tyrosine,","
R5729 T11304 T11321 arg2Of tyrosine,diluted
R5730 T11308 T11307 arg1Of antibody,STAT1
R5731 T11308 T11309 arg1Of antibody,","
R5732 T11309 T11313 arg1Of ",",and
R5733 T11311 T11309 arg2Of antibody,","
R5734 T11311 T11310 arg1Of antibody,anti-STAT3
R5735 T11313 T11306 arg2Of and,of
R5736 T11313 T11312 arg1Of and,","
R5737 T11313 T11317 arg1Of and,of
R5738 T11315 T11313 arg2Of tyrosine,and
R5739 T11315 T11314 arg1Of tyrosine,anti-phosphorylated
R5740 T11315 T11316 arg1Of tyrosine,705
R5741 T11319 T11317 arg2Of antibody,of
R5742 T11319 T11318 arg1Of antibody,STAT3
R5743 T11321 T11322 arg1Of diluted,as
R5744 T11323 T11322 arg2Of recommended,as
R5745 T11323 T11324 arg1Of recommended,at
R5746 T11325 T11324 arg2Of 1/2000,at
R5747 T11325 T11326 arg1Of 1/2000,with
R5748 T11328 T11326 arg2Of saline,with
R5749 T11328 T11327 arg1Of saline,Tris-buffered
R5750 T11328 T11329 arg1Of saline,with
R5751 T11328 T11334 arg1Of saline,with
R5752 T11331 T11330 arg1Of %,0.1
R5753 T11333 T11329 arg2Of 20,with
R5754 T11333 T11331 arg1Of 20,%
R5755 T11333 T11332 arg1Of 20,Tween
R5756 T11335 T11336 arg1Of 5,%
R5757 T11337 T11334 arg2Of BSA,with
R5758 T11337 T11335 arg1Of BSA,5
R5759 T11339 T11338 arg1Of binding,Antibody
R5760 T11339 T11340 arg1Of binding,was
R5761 T11339 T11341 arg2Of binding,detected
R5762 T11339 T11365 arg1Of binding,was
R5763 T11339 T11366 arg2Of binding,revealed
R5764 T11341 T11340 arg2Of detected,was
R5765 T11341 T11364 arg1Of detected,and
R5766 T11347 T11341 arg1Of antibody,detected
R5767 T11347 T11342 arg2Of antibody,by
R5768 T11347 T11343 arg1Of antibody,horseradish
R5769 T11347 T11344 arg1Of antibody,peroxidase-conjugated
R5770 T11347 T11345 arg1Of antibody,anti-rabbit
R5771 T11347 T11346 arg1Of antibody,IgG
R5772 T11347 T11348 arg2Of antibody,diluted
R5773 T11348 T11349 arg1Of diluted,at
R5774 T11350 T11349 arg2Of 1/4000,at
R5775 T11350 T11351 arg1Of 1/4000,with
R5776 T11353 T11351 arg2Of saline,with
R5777 T11353 T11352 arg1Of saline,Tris-buffered
R5778 T11353 T11354 arg1Of saline,with
R5779 T11356 T11355 arg1Of %,0.1
R5780 T11358 T11359 arg1Of 20,with
R5781 T11361 T11359 arg2Of %,with
R5782 T11361 T11360 arg1Of %,5
R5783 T11362 T11354 arg2Of BSA,with
R5784 T11362 T11356 arg1Of BSA,%
R5785 T11362 T11357 arg1Of BSA,Tween
R5786 T11362 T11358 arg1Of BSA,20
R5787 T11364 T11363 arg1Of and,","
R5788 T11366 T11364 arg2Of revealed,and
R5789 T11366 T11365 arg2Of revealed,was
R5790 T11366 T11367 modOf revealed,using
R5791 T11370 T11367 arg2Of system,using
R5792 T11370 T11368 arg1Of system,the
R5793 T11370 T11369 arg1Of system,chemiluminescence
R5794 T11372 T11371 arg1Of bands,Protein
R5795 T11372 T11373 arg1Of bands,were
R5796 T11372 T11374 arg2Of bands,quantified
R5797 T11374 T11373 arg2Of quantified,were
R5798 T11374 T11377 modOf quantified,using
R5799 T11374 T11381 arg1Of quantified,and
R5800 T11376 T11374 arg1Of densitometry,quantified
R5801 T11376 T11375 arg2Of densitometry,by
R5802 T11379 T11377 arg2Of analysis,using
R5803 T11379 T11378 arg1Of analysis,NIH-Image
R5804 T11381 T11380 arg1Of and,","
R5805 T11383 T11382 arg1Of phosphorylation,STAT
R5806 T11383 T11384 arg1Of phosphorylation,was
R5807 T11383 T11385 arg2Of phosphorylation,compared
R5808 T11385 T11381 arg2Of compared,and
R5809 T11385 T11384 arg2Of compared,was
R5810 T11385 T11386 arg1Of compared,with
R5811 T11389 T11386 arg2Of amount,with
R5812 T11389 T11387 arg1Of amount,the
R5813 T11389 T11388 arg1Of amount,total
R5814 T11389 T11390 arg1Of amount,of
R5815 T11392 T11390 arg2Of protein,of
R5816 T11392 T11391 arg1Of protein,STAT
R5817 T11395 T11393 arg1Of cells,IFN-γ-stimulated
R5818 T11395 T11394 arg1Of cells,Hela
R5819 T11395 T11396 arg1Of cells,were
R5820 T11395 T11397 arg2Of cells,used
R5821 T11397 T11396 arg2Of used,were
R5822 T11397 T11398 arg1Of used,as
R5823 T11401 T11398 arg2Of control,as
R5824 T11401 T11399 arg1Of control,a
R5825 T11401 T11400 arg1Of control,positive
R5826 T11401 T11402 arg1Of control,for
R5827 T11404 T11402 arg2Of phosophorylation,for
R5828 T11404 T11403 arg1Of phosophorylation,STAT1

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T10978 1805-1810 Protein denotes STAT1
T10977 1741-1746 Protein denotes IFN-γ
T10976 1668-1672 Protein denotes STAT
T10975 1264-1269 Protein denotes STAT3
T10974 1208-1213 Protein denotes STAT3
T10973 1187-1192 Protein denotes STAT1
T10972 1135-1140 Protein denotes STAT1
T10971 918-923 Protein denotes STAT3
T10970 908-913 Protein denotes STAT1
T10969 369-373 Protein denotes IL-6
T10968 249-252 Protein denotes CD4
T10967 212-216 Protein denotes IL-6
T10966 107-111 Protein denotes IL-6
T10965 97-102 Protein denotes IL-10
T10964 22-25 Protein denotes CD4

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T11067 1741-1746 http://www.uniprot.org/uniprot/P01579 denotes IFN-γ
T11066 1264-1269 http://www.uniprot.org/uniprot/P40763 denotes STAT3
T11065 1208-1213 http://www.uniprot.org/uniprot/P40763 denotes STAT3
T11064 918-923 http://www.uniprot.org/uniprot/P40763 denotes STAT3
T11063 369-373 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T11062 212-216 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T11061 107-111 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T11060 97-102 http://www.uniprot.org/uniprot/P22301 denotes IL-10
T11059 249-252 http://www.uniprot.org/uniprot/P01730 denotes CD4
T11058 22-25 http://www.uniprot.org/uniprot/P01730 denotes CD4

UBERON-AE

Id Subject Object Predicate Lexical cue
T10650 831-835 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk
T10649 308-313 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T10648 206-211 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T10939 1673-1688 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T10938 889-904 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T10937 225-240 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation

GO-CC

Id Subject Object Predicate Lexical cue
T11010 1450-1462 http://purl.obolibrary.org/obo/GO_0071736 denotes IgG antibody
T11009 1074-1082 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T11008 797-805 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T11007 746-755 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T11006 1454-1462 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11005 1270-1278 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11004 1214-1222 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11003 1193-1201 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11002 1141-1149 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11001 385-393 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T11000 1454-1462 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10999 1270-1278 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10998 1214-1222 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10997 1193-1201 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10996 1141-1149 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10995 385-393 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T10994 1763-1768 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10993 502-507 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10992 256-261 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10991 136-141 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10990 29-34 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GO-MF

Id Subject Object Predicate Lexical cue
T10989 1380-1387 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T10988 1454-1462 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10987 1270-1278 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10986 1214-1222 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10985 1193-1201 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10984 1141-1149 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10983 385-393 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T10982 369-373 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T10981 212-216 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T10980 107-111 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T10979 97-102 http://purl.obolibrary.org/obo/GO_0005141 denotes IL-10

sentences

Id Subject Object Predicate Lexical cue
T10936 1741-1828 Sentence denotes IFN-γ-stimulated Hela cells were used as a positive control for STAT1 phosophorylation.
T10935 1592-1740 Sentence denotes Protein bands were quantified by densitometry using NIH-Image analysis, and STAT phosphorylation was compared with the total amount of STAT protein.
T10934 1371-1591 Sentence denotes Antibody binding was detected by horseradish peroxidase-conjugated anti-rabbit IgG antibody diluted at 1/4000 with Tris-buffered saline with 0.1% Tween 20 with 5% BSA, and was revealed using the chemiluminescence system.
T10933 1061-1370 Sentence denotes Briefly, the membrane was incubated with the antibodies (rabbit IgG) anti-STAT1 antibody, anti-phosphorylated tyrosine 701 of STAT1 antibody, anti-STAT3 antibody, and anti-phosphorylated tyrosine 705 of STAT3 antibody, diluted as recommended at 1/2000 with Tris-buffered saline with 0.1% Tween 20 with 5% BSA.
T10932 880-1060 Sentence denotes Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology, Beverly, MA, USA) according to the manufacturer's instructions.
T10931 631-879 Sentence denotes Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20.
T10930 458-630 Sentence denotes Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue).
T10929 181-457 Sentence denotes To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne).
T10928 22-180 Sentence denotes CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS.
T10927 0-21 Sentence denotes Western blot analysis
T77 0-21 Sentence denotes Western blot analysis
T78 22-180 Sentence denotes CD4+ T cells were stimulated for 20 min by the indicated concentrations of IL-10 and IL-6 at a density of 5 × 105 cells in 0.5 ml culture medium with 10% FCS.
T79 181-457 Sentence denotes To examine the effect of serum IL-6 on STAT phosphorylation, normal CD4+ T cells were stimulated for 20 min with 30% active RA serum in culture medium with 40 μg/ml neutralizing goat anti-IL-6 polyclonal antibody (IgG; Techne, Princeton, NJ, USA) or control goat IgG (Techne).
T80 458-630 Sentence denotes Whole cell lysates were prepared by placing cells in 100 μl SDS lysing buffer (62.5 mM Tris–HCl [pH 6.8], 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue).
T81 631-879 Sentence denotes Then 20 μl protein samples were fractionated on 10% SDS-polyacrylamide gels and were transferred to nitrocellulose membranes (Amersham, Buckinghamshire, UK), and the membrane was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween 20.
T82 880-1060 Sentence denotes Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology, Beverly, MA, USA) according to the manufacturer's instructions.
T83 1061-1370 Sentence denotes Briefly, the membrane was incubated with the antibodies (rabbit IgG) anti-STAT1 antibody, anti-phosphorylated tyrosine 701 of STAT1 antibody, anti-STAT3 antibody, and anti-phosphorylated tyrosine 705 of STAT3 antibody, diluted as recommended at 1/2000 with Tris-buffered saline with 0.1% Tween 20 with 5% BSA.
T84 1371-1591 Sentence denotes Antibody binding was detected by horseradish peroxidase-conjugated anti-rabbit IgG antibody diluted at 1/4000 with Tris-buffered saline with 0.1% Tween 20 with 5% BSA, and was revealed using the chemiluminescence system.
T85 1592-1740 Sentence denotes Protein bands were quantified by densitometry using NIH-Image analysis, and STAT phosphorylation was compared with the total amount of STAT protein.
T86 1741-1828 Sentence denotes IFN-γ-stimulated Hela cells were used as a positive control for STAT1 phosophorylation.

simple1

Id Subject Object Predicate Lexical cue
T11082 1805-1810 Protein denotes STAT1
T11081 1741-1746 Protein denotes IFN-γ
T11080 1668-1672 Protein denotes STAT
T11079 1264-1269 Protein denotes STAT3
T11078 1208-1213 Protein denotes STAT3
T11077 1187-1192 Protein denotes STAT1
T11076 1135-1140 Protein denotes STAT1
T11075 918-923 Protein denotes STAT3
T11074 908-913 Protein denotes STAT1
T11073 369-373 Protein denotes IL-6
T11072 249-252 Protein denotes CD4
T11071 212-216 Protein denotes IL-6
T11070 107-111 Protein denotes IL-6
T11069 97-102 Protein denotes IL-10
T11068 22-25 Protein denotes CD4

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T11911 1811-1827 Phosphorylation denotes phosophorylation
T11910 1793-1800 Regulation denotes control
T11909 1673-1688 Phosphorylation denotes phosphorylation
T11908 889-904 Phosphorylation denotes phosphorylation
T11907 1805-1810 Protein denotes STAT1
T11906 1741-1746 Protein denotes IFN-γ
T11905 1668-1672 Protein denotes STAT
T11904 1135-1140 Protein denotes STAT1
T11903 1264-1269 Protein denotes STAT3
T11902 1208-1213 Protein denotes STAT3
T11901 1187-1192 Protein denotes STAT1
T11900 918-923 Protein denotes STAT3
T11899 908-913 Protein denotes STAT1
T11898 369-373 Protein denotes IL-6
T11897 249-252 Protein denotes CD4
T11896 212-216 Protein denotes IL-6
T11895 107-111 Protein denotes IL-6
T11894 97-102 Protein denotes IL-10
T11893 22-25 Protein denotes CD4
R6204 T11899 T11908 themeOf STAT1,phosphorylation
R6205 T11900 T11908 themeOf STAT3,phosphorylation
R6206 T11905 T11909 themeOf STAT,phosphorylation
R6207 T11907 T11910 themeOf STAT1,control
R6208 T11907 T11911 themeOf STAT1,phosophorylation

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T11419 1805-1810 Protein denotes STAT1
T11422 1811-1827 Phosphorylation denotes phosophorylation
T11421 1673-1688 Phosphorylation denotes phosphorylation
T11420 889-904 Phosphorylation denotes phosphorylation
T11418 1741-1746 Protein denotes IFN-γ
T11417 1668-1672 Protein denotes STAT
T11416 1135-1140 Protein denotes STAT1
T11415 1264-1269 Protein denotes STAT3
T11414 1208-1213 Protein denotes STAT3
T11413 1187-1192 Protein denotes STAT1
T11412 918-923 Protein denotes STAT3
T11411 908-913 Protein denotes STAT1
T11410 369-373 Protein denotes IL-6
T11409 249-252 Protein denotes CD4
T11408 212-216 Protein denotes IL-6
T11407 107-111 Protein denotes IL-6
T11406 97-102 Protein denotes IL-10
T11405 22-25 Protein denotes CD4
R5829 T11411 T11420 themeOf STAT1,phosphorylation
R5830 T11412 T11420 themeOf STAT3,phosphorylation
R5831 T11417 T11421 themeOf STAT,phosphorylation
R5832 T11419 T11422 themeOf STAT1,phosophorylation

DLUT931

Id Subject Object Predicate Lexical cue
T11777 1811-1827 Phosphorylation denotes phosophorylation
T11776 1673-1688 Phosphorylation denotes phosphorylation
T11775 1151-1170 Phosphorylation denotes anti-phosphorylated
T11774 889-904 Phosphorylation denotes phosphorylation
T11773 1805-1810 Protein denotes STAT1
T11772 1741-1746 Protein denotes IFN-γ
T11771 1668-1672 Protein denotes STAT
T11770 1135-1140 Protein denotes STAT1
T11769 1264-1269 Protein denotes STAT3
T11768 1208-1213 Protein denotes STAT3
T11767 1187-1192 Protein denotes STAT1
T11766 918-923 Protein denotes STAT3
T11765 908-913 Protein denotes STAT1
T11764 369-373 Protein denotes IL-6
T11763 249-252 Protein denotes CD4
T11762 212-216 Protein denotes IL-6
T11761 107-111 Protein denotes IL-6
T11760 97-102 Protein denotes IL-10
T11759 22-25 Protein denotes CD4
R6169 T11765 T11774 themeOf STAT1,phosphorylation
R6170 T11766 T11774 themeOf STAT3,phosphorylation
R6171 T11767 T11775 themeOf STAT1,anti-phosphorylated
R6172 T11771 T11776 themeOf STAT,phosphorylation
R6173 T11773 T11777 themeOf STAT1,phosophorylation

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T11860 1318-1369 Entity denotes Tris-buffered saline with 0.1% Tween 20 with 5% BSA
T11859 1741-1746 Protein denotes IFN-γ
T11858 1228-1279 Entity denotes anti-phosphorylated tyrosine 705 of STAT3 antibody,
T11857 1203-1222 Protein denotes anti-STAT3 antibody
T11856 1151-1279 Entity denotes anti-phosphorylated tyrosine 701 of STAT1 antibody, anti-STAT3 antibody, and anti-phosphorylated tyrosine 705 of STAT3 antibody,
T11855 97-102 Protein denotes IL-10
T11854 1318-1331 Entity denotes Tris-buffered
T11853 511-521 Protein denotes 100 μl SDS
T11852 1102-1129 Entity denotes the antibodies (rabbit IgG)
T11851 1344-1369 Entity denotes 0.1% Tween 20 with 5% BSA
T11850 1264-1279 Protein denotes STAT3 antibody,
T11849 1130-1150 Entity denotes anti-STAT1 antibody,
T11848 683-686 Protein denotes SDS
T11847 567-585 Protein denotes SDS, 10% glycerol,
T11846 679-706 Entity denotes 10% SDS-polyacrylamide gels
T11845 1668-1672 Protein denotes STAT
T11844 545-553 Entity denotes Tris–HCl
T11843 839-859 Entity denotes Tris-buffered saline
T11842 1531-1538 Protein denotes 5% BSA,
T11841 687-701 Entity denotes polyacrylamide
T11840 1747-1768 Entity denotes stimulated Hela cells
T11839 564-629 Entity denotes 2% SDS, 10% glycerol, 50 mM dithiothreitol, 0.1% bromphenol blue)
T11838 220-224 Protein denotes STAT
T11837 374-393 Protein denotes polyclonal antibody
T11836 125-141 Entity denotes of 5 × 105 cells
T11835 1486-1516 Entity denotes Tris-buffered saline with 0.1%
T11834 305-307 Protein denotes RA
T11833 757-786 Protein denotes Amersham, Buckinghamshire, UK
T11832 550-553 Entity denotes HCl
T11831 918-923 Protein denotes STAT3
T11830 572-584 Entity denotes 10% glycerol
T11829 1203-1222 Entity denotes anti-STAT3 antibody
T11828 1371-1379 Protein denotes Antibody
T11827 502-507 Entity denotes cells
T11892 1668-1739 Phosphorylation denotes STAT phosphorylation was compared with the total amount of STAT protein
T11891 880-996 Phosphorylation denotes Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,
T11890 880-996 Phosphorylation denotes Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,
T11889 217-240 Phosphorylation denotes on STAT phosphorylation
T11888 181-241 Regulation denotes To examine the effect of serum IL-6 on STAT phosphorylation,
T11887 880-888 Entity denotes Tyrosine
T11886 1130-1150 Protein denotes anti-STAT1 antibody,
T11885 970-974 Entity denotes Cell
T11884 1187-1192 Protein denotes STAT1
T11883 522-528 Protein denotes lysing
T11882 1592-1637 Entity denotes Protein bands were quantified by densitometry
T11881 1070-1082 Entity denotes the membrane
T11880 545-553 Entity denotes Tris–HCl
T11879 555-559 Entity denotes pH 6
T11878 22-34 Entity denotes CD4+ T cells
T11877 793-805 Entity denotes the membrane
T11876 731-745 Entity denotes nitrocellulose
T11875 107-111 Protein denotes IL-6
T11874 908-913 Protein denotes STAT1
T11873 839-852 Entity denotes Tris-buffered
T11872 1486-1499 Entity denotes Tris-buffered
T11871 1805-1810 Protein denotes STAT1
T11870 242-261 Entity denotes normal CD4+ T cells
T11869 642-657 Protein denotes protein samples
T11868 1404-1426 Protein denotes horseradish peroxidase
T11867 1264-1269 Protein denotes STAT3
T11866 203-216 Protein denotes of serum IL-6
T11865 1187-1201 Entity denotes STAT1 antibody
T11864 1724-1739 Protein denotes of STAT protein
T11863 1727-1731 Protein denotes STAT
T11862 731-787 Entity denotes nitrocellulose membranes (Amersham, Buckinghamshire, UK)
T11861 1363-1369 Protein denotes 5% BSA
R6194 T11831 T11890 themeOf STAT3,"Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,"
R6195 T11838 T11889 themeOf STAT,on STAT phosphorylation
R6196 T11845 T11892 themeOf STAT,STAT phosphorylation was compared with the total amount of STAT protein
R6197 T11866 T11888 causeOf of serum IL-6,"To examine the effect of serum IL-6 on STAT phosphorylation,"
R6198 T11874 T11891 themeOf STAT1,"Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,"
R6199 T11887 T11874 partOf Tyrosine,STAT1
R6200 T11887 T11831 partOf Tyrosine,STAT3
R6201 T11887 T11890 siteOf Tyrosine,"Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,"
R6202 T11887 T11891 siteOf Tyrosine,"Tyrosine phosphorylation of STAT1 and STAT3 was detected using commercial available kits (Cell Signaling Technology,"
R6203 T11889 T11888 themeOf on STAT phosphorylation,"To examine the effect of serum IL-6 on STAT phosphorylation,"

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T11758 1827-1828 . denotes .
T11757 1811-1827 NN denotes phosophorylation
T11756 1805-1810 CD denotes STAT1
T11755 1801-1804 IN denotes for
T11754 1793-1800 NN denotes control
T11753 1784-1792 JJ denotes positive
T11752 1782-1783 DT denotes a
T11751 1779-1781 IN denotes as
T11750 1774-1778 VBN denotes used
T11749 1769-1773 VBD denotes were
T11748 1763-1768 NNS denotes cells
T11747 1758-1762 NNP denotes Hela
T11746 1741-1757 JJ denotes IFN-γ-stimulated
T11745 1739-1740 . denotes .
T11744 1732-1739 NN denotes protein
T11743 1727-1731 NNP denotes STAT
T11742 1724-1726 IN denotes of
T11741 1717-1723 NN denotes amount
T11740 1711-1716 JJ denotes total
T11739 1707-1710 DT denotes the
T11738 1702-1706 IN denotes with
T11737 1693-1701 VBN denotes compared
T11736 1689-1692 VBD denotes was
T11735 1673-1688 NN denotes phosphorylation
T11734 1668-1672 NNP denotes STAT
T11733 1664-1667 CC denotes and
T11732 1662-1663 , denotes ,
T11731 1654-1662 NN denotes analysis
T11730 1644-1653 JJ denotes NIH-Image
T11729 1638-1643 VBG denotes using
T11728 1625-1637 NN denotes densitometry
T11727 1622-1624 IN denotes by
T11726 1611-1621 VBN denotes quantified
T11725 1606-1610 VBD denotes were
T11724 1600-1605 NNS denotes bands
T11723 1592-1599 NN denotes Protein
T11722 1590-1591 . denotes .
T11721 1584-1590 NN denotes system
T11720 1566-1583 NN denotes chemiluminescence
T11719 1562-1565 DT denotes the
T11718 1556-1561 VBG denotes using
T11717 1547-1555 VBN denotes revealed
T11716 1543-1546 VBD denotes was
T11715 1539-1542 CC denotes and
T11714 1537-1538 , denotes ,
T11713 1534-1537 NNP denotes BSA
T11712 1532-1533 NN denotes %
T11711 1531-1532 CD denotes 5
T11710 1526-1530 IN denotes with
T11709 1523-1525 CD denotes 20
T11708 1517-1522 CD denotes Tween
T11707 1515-1516 NN denotes %
T11706 1512-1515 CD denotes 0.1
T11705 1507-1511 IN denotes with
T11704 1500-1506 NN denotes saline
T11703 1486-1499 JJ denotes Tris-buffered
T11702 1481-1485 IN denotes with
T11701 1474-1480 CD denotes 1/4000
T11700 1471-1473 IN denotes at
T11699 1463-1470 VBN denotes diluted
T11698 1454-1462 NN denotes antibody
T11697 1450-1453 NNP denotes IgG
T11696 1438-1449 JJ denotes anti-rabbit
T11695 1416-1437 JJ denotes peroxidase-conjugated
T11694 1404-1415 JJ denotes horseradish
T11693 1401-1403 IN denotes by
T11692 1392-1400 VBN denotes detected
T11691 1388-1391 VBD denotes was
T11690 1380-1387 JJ denotes binding
T11689 1371-1379 NN denotes Antibody
T11688 1369-1370 . denotes .
T11687 1366-1369 NNP denotes BSA
T11686 1364-1365 NN denotes %
T11685 1363-1364 CD denotes 5
T11684 1358-1362 IN denotes with
T11683 1355-1357 CD denotes 20
T11682 1349-1354 CD denotes Tween
T11681 1347-1348 NN denotes %
T11680 1344-1347 CD denotes 0.1
T11679 1339-1343 IN denotes with
T11678 1332-1338 NN denotes saline
T11677 1318-1331 JJ denotes Tris-buffered
T11676 1313-1317 IN denotes with
T11675 1306-1312 CD denotes 1/2000
T11674 1303-1305 IN denotes at
T11673 1291-1302 VBN denotes recommended
T11672 1288-1290 IN denotes as
T11671 1280-1287 VBN denotes diluted
T11670 1278-1279 , denotes ,
T11669 1270-1278 NN denotes antibody
T11668 1264-1269 CD denotes STAT3
T11667 1261-1263 IN denotes of
T11666 1257-1260 CD denotes 705
T11665 1248-1256 NN denotes tyrosine
T11664 1228-1247 JJ denotes anti-phosphorylated
T11663 1224-1227 CC denotes and
T11662 1222-1223 , denotes ,
T11661 1214-1222 NN denotes antibody
T11660 1203-1213 JJ denotes anti-STAT3
T11659 1201-1202 , denotes ,
T11658 1193-1201 NN denotes antibody
T11657 1187-1192 CD denotes STAT1
T11656 1184-1186 IN denotes of
T11655 1180-1183 CD denotes 701
T11654 1171-1179 NN denotes tyrosine
T11653 1151-1170 JJ denotes anti-phosphorylated
T11652 1149-1150 , denotes ,
T11651 1141-1149 NN denotes antibody
T11650 1130-1140 JJ denotes anti-STAT1
T11649 1128-1129 -RRB- denotes )
T11648 1125-1128 NNP denotes IgG
T11647 1118-1124 NN denotes rabbit
T11646 1117-1118 -LRB- denotes (
T11645 1106-1116 NNS denotes antibodies
T11644 1102-1105 DT denotes the
T11643 1097-1101 IN denotes with
T11642 1087-1096 VBN denotes incubated
T11641 1083-1086 VBD denotes was
T11640 1074-1082 NN denotes membrane
T11639 1070-1073 DT denotes the
T11638 1068-1069 , denotes ,
T11637 1061-1068 RB denotes Briefly
T11636 1059-1060 . denotes .
T11635 1047-1059 NNS denotes instructions
T11634 1044-1046 POS denotes 's
T11633 1032-1044 NN denotes manufacturer
T11632 1028-1031 DT denotes the
T11631 1025-1027 TO denotes to
T11630 1015-1024 VBG denotes according
T11629 1013-1014 -RRB- denotes )
T11628 1010-1013 NNP denotes USA
T11627 1008-1009 , denotes ,
T11626 1006-1008 NNP denotes MA
T11625 1004-1005 , denotes ,
T11624 997-1004 NNP denotes Beverly
T11623 995-996 , denotes ,
T11622 985-995 NNP denotes Technology
T11621 975-984 NNP denotes Signaling
T11620 970-974 NNP denotes Cell
T11619 969-970 -LRB- denotes (
T11618 964-968 NNS denotes kits
T11617 954-963 JJ denotes available
T11616 943-953 JJ denotes commercial
T11615 937-942 VBG denotes using
T11614 928-936 VBN denotes detected
T11613 924-927 VBD denotes was
T11612 918-923 CD denotes STAT3
T11611 914-917 CC denotes and
T11610 908-913 CD denotes STAT1
T11609 905-907 IN denotes of
T11608 889-904 NN denotes phosphorylation
T11604 870-875 CD denotes Tween
T11603 868-869 NN denotes %
T11602 865-868 CD denotes 0.1
T11601 860-864 IN denotes with
T11600 853-859 NN denotes saline
T11599 839-852 JJ denotes Tris-buffered
T11598 836-838 IN denotes in
T11597 831-835 NN denotes milk
T11596 826-830 VBP denotes skim
T11595 824-825 NN denotes %
T11594 823-824 CD denotes 5
T11593 818-822 IN denotes with
T11592 810-817 VBN denotes blocked
T11591 806-809 VBD denotes was
T11590 797-805 NN denotes membrane
T11589 793-796 DT denotes the
T11588 789-792 CC denotes and
T11587 787-788 , denotes ,
T11586 786-787 -RRB- denotes )
T11585 784-786 NNP denotes UK
T11584 782-783 , denotes ,
T11583 767-782 NNP denotes Buckinghamshire
T11582 765-766 , denotes ,
T11581 757-765 NNP denotes Amersham
T11580 756-757 -LRB- denotes (
T11579 746-755 NNS denotes membranes
T11578 731-745 JJ denotes nitrocellulose
T11577 728-730 TO denotes to
T11576 716-727 VBN denotes transferred
T11575 711-715 VBD denotes were
T11574 707-710 CC denotes and
T11573 702-706 NNS denotes gels
T11572 683-701 JJ denotes SDS-polyacrylamide
T11571 681-682 NN denotes %
T11570 679-681 CD denotes 10
T11569 676-678 IN denotes on
T11568 663-675 VBN denotes fractionated
T11567 658-662 VBD denotes were
T11566 650-657 NNS denotes samples
T11565 642-649 NN denotes protein
T11564 639-641 NN denotes μl
T11563 636-638 CD denotes 20
T11562 631-635 RB denotes Then
T11561 629-630 . denotes .
T11560 628-629 -RRB- denotes )
T11559 624-628 JJ denotes blue
T11558 613-623 NN denotes bromphenol
T11557 611-612 NN denotes %
T11556 608-611 CD denotes 0.1
T11555 606-607 , denotes ,
T11554 592-606 NN denotes dithiothreitol
T11553 589-591 NN denotes mM
T11552 586-588 CD denotes 50
T11551 584-585 , denotes ,
T11550 576-584 NN denotes glycerol
T11549 574-575 NN denotes %
T11548 572-574 CD denotes 10
T11547 570-571 , denotes ,
T11546 567-570 NNP denotes SDS
T11545 565-566 NN denotes %
T11544 564-565 CD denotes 2
T11543 562-563 , denotes ,
T11542 561-562 NNP denotes ]
T11541 558-561 CD denotes 6.8
T11540 555-557 NNP denotes pH
T11539 554-555 NNP denotes [
T11538 550-553 NNP denotes HCl
T11537 545-549 NNP denotes Tris
T11536 542-544 NNP denotes mM
T11535 537-541 CD denotes 62.5
T11534 536-537 -LRB- denotes (
T11533 529-535 NN denotes buffer
T11532 522-528 VBG denotes lysing
T11531 518-521 NNP denotes SDS
T11530 515-517 NN denotes μl
T11529 511-514 CD denotes 100
T11528 508-510 IN denotes in
T11527 502-507 NNS denotes cells
T11526 494-501 VBG denotes placing
T11525 491-493 IN denotes by
T11524 482-490 VBN denotes prepared
T11523 477-481 VBD denotes were
T11522 469-476 NNS denotes lysates
T11521 464-468 NN denotes cell
T11520 458-463 JJ denotes Whole
T11519 456-457 . denotes .
T11518 455-456 -RRB- denotes )
T11517 449-455 NNP denotes Techne
T11516 448-449 -LRB- denotes (
T11515 444-447 NNP denotes IgG
T11514 439-443 JJ denotes goat
T11513 431-438 VB denotes control
T11512 428-430 CC denotes or
T11511 426-427 -RRB- denotes )
T11510 423-426 NNP denotes USA
T11509 421-422 , denotes ,
T11508 419-421 NNP denotes NJ
T11507 417-418 , denotes ,
T11506 408-417 NNP denotes Princeton
T11505 406-407 , denotes ,
T11504 400-406 NNP denotes Techne
T11503 398-399 : denotes ;
T11502 395-398 NNP denotes IgG
T11501 394-395 -LRB- denotes (
T11500 385-393 NN denotes antibody
T11499 374-384 JJ denotes polyclonal
T11498 364-373 JJ denotes anti-IL-6
T11497 359-363 NN denotes goat
T11496 346-358 VBG denotes neutralizing
T11495 343-345 NN denotes ml
T11494 342-343 NN denotes /
T11493 340-342 NN denotes μg
T11492 337-339 CD denotes 40
T11491 332-336 IN denotes with
T11490 325-331 NN denotes medium
T11489 317-324 NN denotes culture
T11488 314-316 IN denotes in
T11487 308-313 NN denotes serum
T11486 305-307 NNP denotes RA
T11485 298-304 JJ denotes active
T11484 296-297 NN denotes %
T11483 294-296 CD denotes 30
T11482 289-293 IN denotes with
T11481 285-288 NN denotes min
T11480 282-284 CD denotes 20
T11479 278-281 IN denotes for
T11478 267-277 VBN denotes stimulated
T11477 262-266 VBD denotes were
T11476 256-261 NNS denotes cells
T11475 254-255 NNP denotes T
T11474 252-253 CD denotes +
T11473 249-252 NNP denotes CD4
T11472 242-248 JJ denotes normal
T11471 240-241 , denotes ,
T11470 225-240 NN denotes phosphorylation
T11469 220-224 NNP denotes STAT
T11468 217-219 IN denotes on
T11467 212-216 NN denotes IL-6
T11466 206-211 NN denotes serum
T11465 203-205 IN denotes of
T11464 196-202 NN denotes effect
T11463 192-195 DT denotes the
T11462 184-191 VB denotes examine
T11461 181-183 TO denotes To
T11460 179-180 . denotes .
T11459 176-179 NNP denotes FCS
T11458 174-175 NN denotes %
T11457 172-174 CD denotes 10
T11456 167-171 IN denotes with
T11455 160-166 NN denotes medium
T11454 152-159 NN denotes culture
T11453 149-151 JJ denotes ml
T11452 145-148 CD denotes 0.5
T11451 142-144 IN denotes in
T11450 136-141 NNS denotes cells
T11449 132-135 CD denotes 105
T11448 130-131 CD denotes ×
T11447 128-129 CD denotes 5
T11446 125-127 IN denotes of
T11445 117-124 NN denotes density
T11444 115-116 DT denotes a
T11443 112-114 IN denotes at
T11442 107-111 NN denotes IL-6
T11441 103-106 CC denotes and
T11440 97-102 NN denotes IL-10
T11439 94-96 IN denotes of
T11438 79-93 NNS denotes concentrations
T11437 69-78 VBN denotes indicated
T11436 65-68 DT denotes the
T11435 62-64 IN denotes by
T11434 58-61 NN denotes min
T11433 55-57 CD denotes 20
T11432 51-54 IN denotes for
T11431 40-50 VBN denotes stimulated
T11430 35-39 VBD denotes were
T11429 29-34 NNS denotes cells
T11428 27-28 NNP denotes T
T11427 25-26 NNP denotes +
T11426 22-25 NNP denotes CD4
T11425 13-21 NN denotes analysis
T11424 8-12 NN denotes blot
T11423 0-7 JJ denotes Western
T11607 880-888 NNP denotes Tyrosine
T11606 878-879 . denotes .
T11605 876-878 CD denotes 20
R5833 T11423 T11425 amod Western,analysis
R5834 T11424 T11425 compound blot,analysis
R5835 T11425 T11429 compound analysis,cells
R5836 T11426 T11429 compound CD4,cells
R5837 T11427 T11429 compound +,cells
R5838 T11428 T11429 compound T,cells
R5839 T11429 T11431 nsubjpass cells,stimulated
R5840 T11430 T11431 auxpass were,stimulated
R5841 T11431 T11431 ROOT stimulated,stimulated
R5842 T11432 T11431 prep for,stimulated
R5843 T11433 T11434 nummod 20,min
R5844 T11434 T11432 pobj min,for
R5845 T11435 T11434 prep by,min
R5846 T11436 T11438 det the,concentrations
R5847 T11437 T11438 amod indicated,concentrations
R5848 T11438 T11435 pobj concentrations,by
R5849 T11439 T11438 prep of,concentrations
R5850 T11440 T11439 pobj IL-10,of
R5851 T11441 T11440 cc and,IL-10
R5852 T11442 T11440 conj IL-6,IL-10
R5853 T11443 T11438 prep at,concentrations
R5854 T11444 T11445 det a,density
R5855 T11445 T11443 pobj density,at
R5856 T11446 T11445 prep of,density
R5857 T11447 T11450 nummod 5,cells
R5858 T11448 T11450 nummod ×,cells
R5859 T11449 T11450 nummod 105,cells
R5860 T11450 T11446 pobj cells,of
R5861 T11451 T11438 prep in,concentrations
R5862 T11452 T11455 nummod 0.5,medium
R5863 T11453 T11455 amod ml,medium
R5864 T11454 T11455 compound culture,medium
R5865 T11455 T11451 pobj medium,in
R5866 T11456 T11455 prep with,medium
R5867 T11457 T11458 nummod 10,%
R5868 T11458 T11459 compound %,FCS
R5869 T11459 T11456 pobj FCS,with
R5870 T11460 T11431 punct .,stimulated
R5871 T11461 T11462 aux To,examine
R5872 T11462 T11478 advcl examine,stimulated
R5873 T11463 T11464 det the,effect
R5874 T11464 T11462 dobj effect,examine
R5875 T11465 T11464 prep of,effect
R5876 T11466 T11467 compound serum,IL-6
R5877 T11467 T11465 pobj IL-6,of
R5878 T11468 T11464 prep on,effect
R5879 T11469 T11470 compound STAT,phosphorylation
R5880 T11470 T11468 pobj phosphorylation,on
R5881 T11471 T11478 punct ",",stimulated
R5882 T11472 T11476 amod normal,cells
R5883 T11473 T11476 compound CD4,cells
R5884 T11474 T11476 nummod +,cells
R5885 T11475 T11476 compound T,cells
R5886 T11476 T11478 nsubjpass cells,stimulated
R5887 T11477 T11478 auxpass were,stimulated
R5888 T11478 T11478 ROOT stimulated,stimulated
R5889 T11479 T11478 prep for,stimulated
R5890 T11480 T11481 nummod 20,min
R5891 T11481 T11479 pobj min,for
R5892 T11482 T11481 prep with,min
R5893 T11483 T11484 nummod 30,%
R5894 T11484 T11487 nmod %,serum
R5895 T11485 T11487 amod active,serum
R5896 T11486 T11487 compound RA,serum
R5897 T11487 T11482 pobj serum,with
R5898 T11488 T11487 prep in,serum
R5899 T11489 T11490 compound culture,medium
R5900 T11490 T11488 pobj medium,in
R5901 T11491 T11481 prep with,min
R5902 T11492 T11493 nummod 40,μg
R5903 T11493 T11495 compound μg,ml
R5904 T11494 T11495 compound /,ml
R5905 T11495 T11491 pobj ml,with
R5906 T11496 T11495 acl neutralizing,ml
R5907 T11497 T11500 nmod goat,antibody
R5908 T11498 T11500 nmod anti-IL-6,antibody
R5909 T11499 T11500 amod polyclonal,antibody
R5910 T11500 T11496 dobj antibody,neutralizing
R5911 T11501 T11502 punct (,IgG
R5912 T11502 T11500 appos IgG,antibody
R5913 T11503 T11502 punct ;,IgG
R5914 T11504 T11502 conj Techne,IgG
R5915 T11505 T11504 punct ",",Techne
R5916 T11506 T11504 conj Princeton,Techne
R5917 T11507 T11506 punct ",",Princeton
R5918 T11508 T11506 conj NJ,Princeton
R5919 T11509 T11508 punct ",",NJ
R5920 T11510 T11508 conj USA,NJ
R5921 T11511 T11508 punct ),NJ
R5922 T11512 T11500 cc or,antibody
R5923 T11513 T11500 conj control,antibody
R5924 T11514 T11515 amod goat,IgG
R5925 T11515 T11513 dobj IgG,control
R5926 T11516 T11517 punct (,Techne
R5927 T11517 T11515 appos Techne,IgG
R5928 T11518 T11495 punct ),ml
R5929 T11519 T11478 punct .,stimulated
R5930 T11520 T11522 amod Whole,lysates
R5931 T11521 T11522 compound cell,lysates
R5932 T11522 T11524 nsubjpass lysates,prepared
R5933 T11523 T11524 auxpass were,prepared
R5934 T11524 T11524 ROOT prepared,prepared
R5935 T11525 T11524 prep by,prepared
R5936 T11526 T11525 pcomp placing,by
R5937 T11527 T11526 dobj cells,placing
R5938 T11528 T11526 prep in,placing
R5939 T11529 T11533 nummod 100,buffer
R5940 T11530 T11533 compound μl,buffer
R5941 T11531 T11533 compound SDS,buffer
R5942 T11532 T11533 compound lysing,buffer
R5943 T11533 T11528 pobj buffer,in
R5944 T11534 T11542 punct (,]
R5945 T11535 T11537 nummod 62.5,Tris
R5946 T11536 T11537 compound mM,Tris
R5947 T11537 T11542 nmod Tris,]
R5948 T11538 T11540 compound HCl,pH
R5949 T11539 T11540 compound [,pH
R5950 T11540 T11537 appos pH,Tris
R5951 T11541 T11542 nummod 6.8,]
R5952 T11542 T11526 prep ],placing
R5953 T11543 T11542 punct ",",]
R5954 T11544 T11545 nummod 2,%
R5955 T11545 T11546 compound %,SDS
R5956 T11546 T11542 npadvmod SDS,]
R5957 T11547 T11546 punct ",",SDS
R5958 T11548 T11549 nummod 10,%
R5959 T11549 T11550 compound %,glycerol
R5960 T11550 T11546 appos glycerol,SDS
R5961 T11551 T11550 punct ",",glycerol
R5962 T11552 T11553 nummod 50,mM
R5963 T11553 T11554 compound mM,dithiothreitol
R5964 T11554 T11550 conj dithiothreitol,glycerol
R5965 T11555 T11554 punct ",",dithiothreitol
R5966 T11556 T11557 nummod 0.1,%
R5967 T11557 T11550 appos %,glycerol
R5968 T11558 T11559 compound bromphenol,blue
R5969 T11559 T11557 appos blue,%
R5970 T11560 T11559 punct ),blue
R5971 T11561 T11542 punct .,]
R5972 T11562 T11568 advmod Then,fractionated
R5973 T11563 T11566 nummod 20,samples
R5974 T11564 T11566 compound μl,samples
R5975 T11565 T11566 compound protein,samples
R5976 T11566 T11568 nsubjpass samples,fractionated
R5977 T11567 T11568 auxpass were,fractionated
R5978 T11568 T11568 ROOT fractionated,fractionated
R5979 T11569 T11568 prep on,fractionated
R5980 T11570 T11571 nummod 10,%
R5981 T11571 T11573 nmod %,gels
R5982 T11572 T11573 amod SDS-polyacrylamide,gels
R5983 T11573 T11569 pobj gels,on
R5984 T11574 T11568 cc and,fractionated
R5985 T11575 T11576 auxpass were,transferred
R5986 T11576 T11568 conj transferred,fractionated
R5987 T11577 T11576 prep to,transferred
R5988 T11578 T11579 amod nitrocellulose,membranes
R5989 T11579 T11577 pobj membranes,to
R5990 T11580 T11592 punct (,blocked
R5991 T11581 T11592 nsubjpass Amersham,blocked
R5992 T11582 T11581 punct ",",Amersham
R5993 T11583 T11581 conj Buckinghamshire,Amersham
R5994 T11584 T11583 punct ",",Buckinghamshire
R5995 T11585 T11583 conj UK,Buckinghamshire
R5996 T11586 T11583 punct ),Buckinghamshire
R5997 T11587 T11581 punct ",",Amersham
R5998 T11588 T11581 cc and,Amersham
R5999 T11589 T11590 det the,membrane
R6000 T11590 T11581 conj membrane,Amersham
R6001 T11591 T11592 auxpass was,blocked
R6002 T11592 T11576 parataxis blocked,transferred
R6003 T11593 T11592 prep with,blocked
R6004 T11594 T11595 nummod 5,%
R6005 T11595 T11596 nsubj %,skim
R6006 T11596 T11576 conj skim,transferred
R6007 T11597 T11596 dobj milk,skim
R6008 T11598 T11596 prep in,skim
R6009 T11599 T11600 amod Tris-buffered,saline
R6010 T11600 T11598 pobj saline,in
R6011 T11601 T11596 prep with,skim
R6012 T11602 T11603 nummod 0.1,%
R6013 T11603 T11605 quantmod %,20
R6014 T11604 T11605 nummod Tween,20
R6015 T11605 T11601 pobj 20,with
R6016 T11606 T11568 punct .,fractionated
R6017 T11607 T11608 compound Tyrosine,phosphorylation
R6018 T11608 T11614 nsubjpass phosphorylation,detected
R6019 T11609 T11608 prep of,phosphorylation
R6020 T11610 T11609 pobj STAT1,of
R6021 T11611 T11610 cc and,STAT1
R6022 T11612 T11610 conj STAT3,STAT1
R6023 T11613 T11614 auxpass was,detected
R6024 T11614 T11614 ROOT detected,detected
R6025 T11615 T11614 advcl using,detected
R6026 T11616 T11618 amod commercial,kits
R6027 T11617 T11618 amod available,kits
R6028 T11618 T11615 dobj kits,using
R6029 T11619 T11618 punct (,kits
R6030 T11620 T11622 compound Cell,Technology
R6031 T11621 T11622 compound Signaling,Technology
R6032 T11622 T11618 appos Technology,kits
R6033 T11623 T11622 punct ",",Technology
R6034 T11624 T11622 conj Beverly,Technology
R6035 T11625 T11624 punct ",",Beverly
R6036 T11626 T11624 conj MA,Beverly
R6037 T11627 T11626 punct ",",MA
R6038 T11628 T11626 appos USA,MA
R6039 T11629 T11626 punct ),MA
R6040 T11630 T11614 prep according,detected
R6041 T11631 T11630 prep to,according
R6042 T11632 T11633 det the,manufacturer
R6043 T11633 T11635 poss manufacturer,instructions
R6044 T11634 T11633 case 's,manufacturer
R6045 T11635 T11631 pobj instructions,to
R6046 T11636 T11614 punct .,detected
R6047 T11637 T11641 advmod Briefly,was
R6048 T11638 T11641 punct ",",was
R6049 T11639 T11640 det the,membrane
R6050 T11640 T11641 nsubj membrane,was
R6051 T11641 T11641 ROOT was,was
R6052 T11642 T11641 attr incubated,was
R6053 T11643 T11642 prep with,incubated
R6054 T11644 T11645 det the,antibodies
R6055 T11645 T11643 pobj antibodies,with
R6056 T11646 T11648 punct (,IgG
R6057 T11647 T11648 compound rabbit,IgG
R6058 T11648 T11645 appos IgG,antibodies
R6059 T11649 T11642 punct ),incubated
R6060 T11650 T11651 amod anti-STAT1,antibody
R6061 T11651 T11671 dep antibody,diluted
R6062 T11652 T11654 punct ",",tyrosine
R6063 T11653 T11654 amod anti-phosphorylated,tyrosine
R6064 T11654 T11651 appos tyrosine,antibody
R6065 T11655 T11654 nummod 701,tyrosine
R6066 T11656 T11654 prep of,tyrosine
R6067 T11657 T11658 nummod STAT1,antibody
R6068 T11658 T11656 pobj antibody,of
R6069 T11659 T11658 punct ",",antibody
R6070 T11660 T11661 amod anti-STAT3,antibody
R6071 T11661 T11658 conj antibody,antibody
R6072 T11662 T11661 punct ",",antibody
R6073 T11663 T11661 cc and,antibody
R6074 T11664 T11665 compound anti-phosphorylated,tyrosine
R6075 T11665 T11661 conj tyrosine,antibody
R6076 T11666 T11671 acl 705,diluted
R6077 T11667 T11666 prep of,705
R6078 T11668 T11669 nummod STAT3,antibody
R6079 T11669 T11667 pobj antibody,of
R6080 T11670 T11671 punct ",",diluted
R6081 T11671 T11641 conj diluted,was
R6082 T11672 T11673 mark as,recommended
R6083 T11673 T11671 advcl recommended,diluted
R6084 T11674 T11673 prep at,recommended
R6085 T11675 T11674 pobj 1/2000,at
R6086 T11676 T11675 prep with,1/2000
R6087 T11677 T11678 amod Tris-buffered,saline
R6088 T11678 T11676 pobj saline,with
R6089 T11679 T11673 prep with,recommended
R6090 T11680 T11681 nummod 0.1,%
R6091 T11681 T11679 pobj %,with
R6092 T11682 T11683 nummod Tween,20
R6093 T11683 T11684 nummod 20,with
R6094 T11684 T11679 conj with,with
R6095 T11685 T11686 nummod 5,%
R6096 T11686 T11687 compound %,BSA
R6097 T11687 T11684 pobj BSA,with
R6098 T11688 T11671 punct .,diluted
R6099 T11689 T11690 compound Antibody,binding
R6100 T11690 T11692 nsubjpass binding,detected
R6101 T11691 T11692 auxpass was,detected
R6102 T11692 T11692 ROOT detected,detected
R6103 T11693 T11692 agent by,detected
R6104 T11694 T11698 amod horseradish,antibody
R6105 T11695 T11698 amod peroxidase-conjugated,antibody
R6106 T11696 T11698 amod anti-rabbit,antibody
R6107 T11697 T11698 compound IgG,antibody
R6108 T11698 T11693 pobj antibody,by
R6109 T11699 T11698 acl diluted,antibody
R6110 T11700 T11699 prep at,diluted
R6111 T11701 T11700 pobj 1/4000,at
R6112 T11702 T11701 prep with,1/4000
R6113 T11703 T11704 amod Tris-buffered,saline
R6114 T11704 T11702 pobj saline,with
R6115 T11705 T11699 prep with,diluted
R6116 T11706 T11707 nummod 0.1,%
R6117 T11707 T11705 pobj %,with
R6118 T11708 T11709 nummod Tween,20
R6119 T11709 T11707 prep 20,%
R6120 T11710 T11709 prep with,20
R6121 T11711 T11712 nummod 5,%
R6122 T11712 T11713 compound %,BSA
R6123 T11713 T11710 pobj BSA,with
R6124 T11714 T11707 punct ",",%
R6125 T11715 T11692 cc and,detected
R6126 T11716 T11717 auxpass was,revealed
R6127 T11717 T11692 conj revealed,detected
R6128 T11718 T11717 advcl using,revealed
R6129 T11719 T11721 det the,system
R6130 T11720 T11721 compound chemiluminescence,system
R6131 T11721 T11718 dobj system,using
R6132 T11722 T11692 punct .,detected
R6133 T11723 T11724 compound Protein,bands
R6134 T11724 T11726 nsubjpass bands,quantified
R6135 T11725 T11726 auxpass were,quantified
R6136 T11726 T11726 ROOT quantified,quantified
R6137 T11727 T11726 agent by,quantified
R6138 T11728 T11727 pobj densitometry,by
R6139 T11729 T11726 advcl using,quantified
R6140 T11730 T11731 amod NIH-Image,analysis
R6141 T11731 T11729 dobj analysis,using
R6142 T11732 T11726 punct ",",quantified
R6143 T11733 T11726 cc and,quantified
R6144 T11734 T11735 compound STAT,phosphorylation
R6145 T11735 T11736 nsubj phosphorylation,was
R6146 T11736 T11726 conj was,quantified
R6147 T11737 T11736 prep compared,was
R6148 T11738 T11737 prep with,compared
R6149 T11739 T11741 det the,amount
R6150 T11740 T11741 amod total,amount
R6151 T11741 T11738 pobj amount,with
R6152 T11742 T11741 prep of,amount
R6153 T11743 T11744 compound STAT,protein
R6154 T11744 T11742 pobj protein,of
R6155 T11745 T11736 punct .,was
R6156 T11746 T11748 amod IFN-γ-stimulated,cells
R6157 T11747 T11748 compound Hela,cells
R6158 T11748 T11750 nsubjpass cells,used
R6159 T11749 T11750 auxpass were,used
R6160 T11750 T11750 ROOT used,used
R6161 T11751 T11750 prep as,used
R6162 T11752 T11754 det a,control
R6163 T11753 T11754 amod positive,control
R6164 T11754 T11751 pobj control,as
R6165 T11755 T11754 prep for,control
R6166 T11756 T11757 nummod STAT1,phosophorylation
R6167 T11757 T11755 pobj phosophorylation,for
R6168 T11758 T11750 punct .,used

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T11057 1793-1800 Positive_regulation denotes control
T11056 1811-1827 Phosphorylation denotes phosophorylation
T11055 1805-1810 Protein denotes STAT1
T11054 1741-1746 Protein denotes IFN-γ
T11053 1673-1688 Phosphorylation denotes phosphorylation
T11052 1673-1688 Phosphorylation denotes phosphorylation
T11051 1727-1739 Protein denotes STAT protein
T11050 1668-1672 Protein denotes STAT
T11049 1380-1387 Binding denotes binding
T11048 1404-1462 Protein denotes horseradish peroxidase-conjugated anti-rabbit IgG antibody
T11047 1228-1278 Protein denotes anti-phosphorylated tyrosine 705 of STAT3 antibody
T11046 1203-1222 Protein denotes anti-STAT3 antibody
T11045 1151-1201 Protein denotes anti-phosphorylated tyrosine 701 of STAT1 antibody
T11044 889-904 Phosphorylation denotes phosphorylation
T11043 889-904 Phosphorylation denotes phosphorylation
T11042 880-888 Entity denotes Tyrosine
T11041 918-923 Protein denotes STAT3
T11040 908-913 Protein denotes STAT1
T11039 196-202 Positive_regulation denotes effect
T11038 346-358 Negative_regulation denotes neutralizing
T11037 346-358 Negative_regulation denotes neutralizing
T11036 346-358 Negative_regulation denotes neutralizing
T11035 225-240 Phosphorylation denotes phosphorylation
T11034 444-447 Protein denotes IgG
T11033 395-398 Protein denotes IgG
T11032 359-393 Protein denotes goat anti-IL-6 polyclonal antibody
T11031 249-253 Protein denotes CD4+
T11030 220-224 Protein denotes STAT
T11029 206-216 Protein denotes serum IL-6
T11028 107-111 Protein denotes IL-6
T11027 97-102 Protein denotes IL-10
T11026 22-26 Protein denotes CD4+
R5485 T11030 T11035 themeOf STAT,phosphorylation
R5486 T11032 T11036 themeOf goat anti-IL-6 polyclonal antibody,neutralizing
R5487 T11033 T11037 themeOf IgG,neutralizing
R5488 T11034 T11038 themeOf IgG,neutralizing
R5489 T11035 T11039 themeOf phosphorylation,effect
R5490 T11040 T11043 themeOf STAT1,phosphorylation
R5491 T11040 T11042 partOf STAT1,Tyrosine
R5492 T11041 T11044 themeOf STAT3,phosphorylation
R5493 T11041 T11042 partOf STAT3,Tyrosine
R5494 T11042 T11043 Site Tyrosine,phosphorylation
R5495 T11042 T11044 Site Tyrosine,phosphorylation
R5496 T11048 T11049 themeOf horseradish peroxidase-conjugated anti-rabbit IgG antibody,binding
R5497 T11050 T11052 themeOf STAT,phosphorylation
R5498 T11051 T11053 themeOf STAT protein,phosphorylation
R5499 T11055 T11056 themeOf STAT1,phosophorylation
R5500 T11056 T11057 themeOf phosophorylation,control
R5484 T11029 T11039 causeOf serum IL-6,effect

test3

Id Subject Object Predicate Lexical cue
T10647 1805-1810 Protein denotes STAT1
T10646 1741-1746 Protein denotes IFN-γ
T10645 1673-1688 Phosphorylation denotes phosphorylation
T10644 1668-1672 Protein denotes STAT
T10643 1380-1387 Binding denotes binding
T10642 1264-1269 Protein denotes STAT3
T10641 1208-1213 Protein denotes STAT3
T10640 1187-1192 Protein denotes STAT1
T10639 1135-1140 Protein denotes STAT1
T10638 918-923 Protein denotes STAT3
T10637 908-913 Protein denotes STAT1
T10636 889-904 Phosphorylation denotes phosphorylation
T10635 369-373 Protein denotes IL-6
T10634 249-252 Protein denotes CD4
T10633 225-240 Phosphorylation denotes phosphorylation
T10632 212-216 Protein denotes IL-6
T10631 196-202 Regulation denotes effect
T10630 107-111 Protein denotes IL-6
T10629 97-102 Protein denotes IL-10
T10628 22-25 Protein denotes CD4
T10627 1805-1810 Protein denotes STAT1
T10626 1741-1746 Protein denotes IFN-γ
T10625 1668-1672 Protein denotes STAT
T10624 1264-1269 Protein denotes STAT3
T10623 1208-1213 Protein denotes STAT3
T10622 1187-1192 Protein denotes STAT1
T10621 1135-1140 Protein denotes STAT1
T10620 918-923 Protein denotes STAT3
T10619 908-913 Protein denotes STAT1
T10618 369-373 Protein denotes IL-6
T10617 249-252 Protein denotes CD4
T10616 212-216 Protein denotes IL-6
T10615 107-111 Protein denotes IL-6
T10614 97-102 Protein denotes IL-10
T10613 22-25 Protein denotes CD4
R5470 T10637 T10636 themeOf STAT1,phosphorylation
R5471 T10638 T10636 themeOf STAT3,phosphorylation
R5472 T10644 T10645 themeOf STAT,phosphorylation

testone

Id Subject Object Predicate Lexical cue
T10594 1673-1688 Phosphorylation denotes phosphorylation
T10593 889-904 Phosphorylation denotes phosphorylation
T10592 225-240 Phosphorylation denotes phosphorylation
T10591 196-202 Regulation denotes effect
T10590 1805-1810 Protein denotes STAT1
T10589 1741-1746 Protein denotes IFN-γ
T10588 1668-1672 Protein denotes STAT
T10587 1264-1269 Protein denotes STAT3
T10586 1208-1213 Protein denotes STAT3
T10585 1187-1192 Protein denotes STAT1
T10584 1135-1140 Protein denotes STAT1
T10583 918-923 Protein denotes STAT3
T10582 908-913 Protein denotes STAT1
T10581 369-373 Protein denotes IL-6
T10580 249-252 Protein denotes CD4
T10579 212-216 Protein denotes IL-6
T10578 107-111 Protein denotes IL-6
T10577 97-102 Protein denotes IL-10
T10576 22-25 Protein denotes CD4
R5465 T10579 T10592 themeOf IL-6,phosphorylation
R5466 T10582 T10593 themeOf STAT1,phosphorylation
R5467 T10583 T10593 themeOf STAT3,phosphorylation
R5468 T10588 T10594 themeOf STAT,phosphorylation
R5469 T10592 T10591 themeOf phosphorylation,effect