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{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T9760","span":{"begin":734,"end":739},"obj":"Protein"},{"id":"T9759","span":{"begin":659,"end":664},"obj":"Protein"},{"id":"T9758","span":{"begin":582,"end":589},"obj":"Protein"},{"id":"T9757","span":{"begin":509,"end":516},"obj":"Protein"},{"id":"T9756","span":{"begin":449,"end":454},"obj":"Protein"},{"id":"T9755","span":{"begin":439,"end":444},"obj":"Protein"},{"id":"T9754","span":{"begin":286,"end":291},"obj":"Protein"},{"id":"T9753","span":{"begin":217,"end":222},"obj":"Protein"},{"id":"T9752","span":{"begin":150,"end":157},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3. Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed. The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression. The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T9797","span":{"begin":659,"end":664},"obj":"http://www.uniprot.org/uniprot/O15524"},{"id":"T9796","span":{"begin":439,"end":444},"obj":"http://www.uniprot.org/uniprot/O15524"},{"id":"T9795","span":{"begin":217,"end":222},"obj":"http://www.uniprot.org/uniprot/O15524"},{"id":"T9794","span":{"begin":582,"end":589},"obj":"http://www.uniprot.org/uniprot/P60709"},{"id":"T9793","span":{"begin":509,"end":516},"obj":"http://www.uniprot.org/uniprot/P60709"},{"id":"T9792","span":{"begin":150,"end":157},"obj":"http://www.uniprot.org/uniprot/P60709"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3. Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed. The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression. The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'."}

{"project":"sentences","denotations":[{"id":"T9730","span":{"begin":529,"end":795},"obj":"Sentence"},{"id":"T9729","span":{"begin":425,"end":528},"obj":"Sentence"},{"id":"T9728","span":{"begin":293,"end":424},"obj":"Sentence"},{"id":"T9727","span":{"begin":0,"end":292},"obj":"Sentence"},{"id":"T73","span":{"begin":0,"end":292},"obj":"Sentence"},{"id":"T74","span":{"begin":293,"end":424},"obj":"Sentence"},{"id":"T75","span":{"begin":425,"end":528},"obj":"Sentence"},{"id":"T76","span":{"begin":529,"end":795},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3. Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed. The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression. The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'."}

{"project":"simple1","denotations":[{"id":"T9808","span":{"begin":734,"end":739},"obj":"Protein"},{"id":"T9807","span":{"begin":659,"end":664},"obj":"Protein"},{"id":"T9806","span":{"begin":582,"end":589},"obj":"Protein"},{"id":"T9805","span":{"begin":509,"end":516},"obj":"Protein"},{"id":"T9804","span":{"begin":449,"end":454},"obj":"Protein"},{"id":"T9803","span":{"begin":439,"end":444},"obj":"Protein"},{"id":"T9802","span":{"begin":286,"end":291},"obj":"Protein"},{"id":"T9801","span":{"begin":217,"end":222},"obj":"Protein"},{"id":"T9800","span":{"begin":150,"end":157},"obj":"Protein"}],"text":"The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3. Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed. The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression. The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T10543","span":{"begin":509,"end":516},"obj":"Protein"},{"id":"T10542","span":{"begin":449,"end":454},"obj":"Protein"},{"id":"T10541","span":{"begin":439,"end":444},"obj":"Protein"},{"id":"T10540","span":{"begin":286,"end":291},"obj":"Protein"},{"id":"T10539","span":{"begin":217,"end":222},"obj":"Protein"},{"id":"T10538","span":{"begin":150,"end":157},"obj":"Protein"},{"id":"T10549","span":{"begin":517,"end":527},"obj":"Gene_expression"},{"id":"T10548","span":{"begin":485,"end":496},"obj":"Negative_regulation"},{"id":"T10547","span":{"begin":455,"end":465},"obj":"Gene_expression"},{"id":"T10546","span":{"begin":582,"end":589},"obj":"Protein"},{"id":"T10545","span":{"begin":734,"end":739},"obj":"Protein"},{"id":"T10544","span":{"begin":659,"end":664},"obj":"Protein"}],"relations":[{"id":"R5461","pred":"themeOf","subj":"T10541","obj":"T10547"},{"id":"R5462","pred":"themeOf","subj":"T10542","obj":"T10547"},{"id":"R5463","pred":"themeOf","subj":"T10543","obj":"T10549"},{"id":"R5464","pred":"themeOf","subj":"T10549","obj":"T10548"}],"text":"The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3. Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed. The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression. The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T10125","span":{"begin":517,"end":527},"obj":"Gene_expression"},{"id":"T10124","span":{"begin":455,"end":465},"obj":"Gene_expression"},{"id":"T10123","span":{"begin":582,"end":589},"obj":"Protein"},{"id":"T10122","span":{"begin":734,"end":739},"obj":"Protein"},{"id":"T10121","span":{"begin":659,"end":664},"obj":"Protein"},{"id":"T10120","span":{"begin":509,"end":516},"obj":"Protein"},{"id":"T10119","span":{"begin":449,"end":454},"obj":"Protein"},{"id":"T10118","span":{"begin":439,"end":444},"obj":"Protein"},{"id":"T10117","span":{"begin":286,"end":291},"obj":"Protein"},{"id":"T10116","span":{"begin":217,"end":222},"obj":"Protein"},{"id":"T10115","span":{"begin":150,"end":157},"obj":"Protein"}],"relations":[{"id":"R5105","pred":"themeOf","subj":"T10118","obj":"T10124"},{"id":"R5106","pred":"themeOf","subj":"T10119","obj":"T10124"},{"id":"R5107","pred":"themeOf","subj":"T10120","obj":"T10125"}],"text":"The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3. Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed. The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression. The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'."}

{"project":"DLUT931","denotations":[{"id":"T10479","span":{"begin":582,"end":589},"obj":"Protein"},{"id":"T10478","span":{"begin":734,"end":739},"obj":"Protein"},{"id":"T10477","span":{"begin":659,"end":664},"obj":"Protein"},{"id":"T10476","span":{"begin":509,"end":516},"obj":"Protein"},{"id":"T10475","span":{"begin":449,"end":454},"obj":"Protein"},{"id":"T10474","span":{"begin":439,"end":444},"obj":"Protein"},{"id":"T10473","span":{"begin":286,"end":291},"obj":"Protein"},{"id":"T10472","span":{"begin":217,"end":222},"obj":"Protein"},{"id":"T10471","span":{"begin":150,"end":157},"obj":"Protein"},{"id":"T10481","span":{"begin":517,"end":527},"obj":"Gene_expression"},{"id":"T10480","span":{"begin":455,"end":465},"obj":"Gene_expression"}],"relations":[{"id":"R5451","pred":"themeOf","subj":"T10474","obj":"T10480"},{"id":"R5452","pred":"themeOf","subj":"T10475","obj":"T10480"},{"id":"R5453","pred":"themeOf","subj":"T10476","obj":"T10481"}],"text":"The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3. Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed. The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression. The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'."}

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cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3. Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed. The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression. The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'."}

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T9790","span":{"begin":734,"end":739},"obj":"Protein"},{"id":"T9789","span":{"begin":659,"end":664},"obj":"Protein"},{"id":"T9788","span":{"begin":582,"end":589},"obj":"Protein"},{"id":"T9787","span":{"begin":517,"end":527},"obj":"Gene_expression"},{"id":"T9786","span":{"begin":455,"end":465},"obj":"Gene_expression"},{"id":"T9785","span":{"begin":455,"end":465},"obj":"Gene_expression"},{"id":"T9784","span":{"begin":509,"end":516},"obj":"Protein"},{"id":"T9783","span":{"begin":449,"end":454},"obj":"Protein"},{"id":"T9782","span":{"begin":439,"end":444},"obj":"Protein"},{"id":"T9781","span":{"begin":286,"end":291},"obj":"Protein"},{"id":"T9780","span":{"begin":217,"end":222},"obj":"Protein"},{"id":"T9779","span":{"begin":150,"end":157},"obj":"Protein"}],"relations":[{"id":"R4788","pred":"themeOf","subj":"T9782","obj":"T9785"},{"id":"R4789","pred":"themeOf","subj":"T9783","obj":"T9786"},{"id":"R4790","pred":"themeOf","subj":"T9784","obj":"T9787"}],"text":"The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3. Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed. The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression. The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'."}

{"project":"test3","denotations":[{"id":"T9354","span":{"begin":734,"end":739},"obj":"Protein"},{"id":"T9353","span":{"begin":659,"end":664},"obj":"Protein"},{"id":"T9352","span":{"begin":582,"end":589},"obj":"Protein"},{"id":"T9351","span":{"begin":517,"end":527},"obj":"Gene_expression"},{"id":"T9350","span":{"begin":509,"end":516},"obj":"Protein"},{"id":"T9349","span":{"begin":455,"end":465},"obj":"Gene_expression"},{"id":"T9348","span":{"begin":449,"end":454},"obj":"Protein"},{"id":"T9347","span":{"begin":439,"end":444},"obj":"Protein"},{"id":"T9346","span":{"begin":286,"end":291},"obj":"Protein"},{"id":"T9345","span":{"begin":217,"end":222},"obj":"Protein"},{"id":"T9344","span":{"begin":150,"end":157},"obj":"Protein"},{"id":"T9341","span":{"begin":734,"end":739},"obj":"Protein"},{"id":"T9340","span":{"begin":659,"end":664},"obj":"Protein"},{"id":"T9339","span":{"begin":582,"end":589},"obj":"Protein"},{"id":"T9338","span":{"begin":509,"end":516},"obj":"Protein"},{"id":"T9337","span":{"begin":449,"end":454},"obj":"Protein"},{"id":"T9336","span":{"begin":439,"end":444},"obj":"Protein"},{"id":"T9335","span":{"begin":286,"end":291},"obj":"Protein"},{"id":"T9334","span":{"begin":217,"end":222},"obj":"Protein"},{"id":"T9333","span":{"begin":150,"end":157},"obj":"Protein"}],"relations":[{"id":"R4781","pred":"themeOf","subj":"T9347","obj":"T9349"},{"id":"R4782","pred":"themeOf","subj":"T9348","obj":"T9349"},{"id":"R4783","pred":"themeOf","subj":"T9350","obj":"T9351"}],"text":"The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3. Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed. The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression. The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'."}

{"project":"testone","denotations":[{"id":"T9318","span":{"begin":517,"end":527},"obj":"Gene_expression"},{"id":"T9317","span":{"begin":455,"end":465},"obj":"Gene_expression"},{"id":"T9316","span":{"begin":734,"end":739},"obj":"Protein"},{"id":"T9315","span":{"begin":659,"end":664},"obj":"Protein"},{"id":"T9314","span":{"begin":582,"end":589},"obj":"Protein"},{"id":"T9313","span":{"begin":509,"end":516},"obj":"Protein"},{"id":"T9312","span":{"begin":449,"end":454},"obj":"Protein"},{"id":"T9311","span":{"begin":439,"end":444},"obj":"Protein"},{"id":"T9310","span":{"begin":286,"end":291},"obj":"Protein"},{"id":"T9309","span":{"begin":217,"end":222},"obj":"Protein"},{"id":"T9308","span":{"begin":150,"end":157},"obj":"Protein"}],"relations":[{"id":"R4778","pred":"themeOf","subj":"T9311","obj":"T9317"},{"id":"R4779","pred":"themeOf","subj":"T9312","obj":"T9317"},{"id":"R4780","pred":"themeOf","subj":"T9313","obj":"T9318"}],"text":"The cDNA samples were denatured at 95° C for 10 min, and were then amplified for 40–50 cycles: at 95° C (10 s), at 65° C (15 s), and 72° C (22 s) for β-actin; at 95° C (10 s), at 62° C (15 s), and at 72° C (10 s) for SOCS1; and at 96° C (10 s), at 68° C (15 s), and at 72° C (15 s) for SOCS3. Amplification curves of the fluorescence values versus cycle number were obtained, and a melting curve analysis was then performed. The levels of SOCS1 and SOCS3 expression were determined by normalizing relative to β-actin expression. The forward and reverse primers were as follows: for β-actin, 5'-GTGGGGCGCCCCAGGCACCA-3' and 5'-CTCCTTAATGTCACGCACGATTTC-3' ; for SOCS1, 5'-AGACCCCTTCTCACCTCTTG-3' and 5'-GCACAGCAGAAAAATAAAGC-3' ; and for SOCS3, 5'-CCCGCCGGCACCTTTCTG-3' and 5'-AGGGGCCGGCTCAACACC-3'."}