PMC:1064873 / 11394-11749 JSONTXT

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    LappsTest

    {"project":"LappsTest","denotations":[{"id":"T9320","span":{"begin":214,"end":264},"obj":"Protein"},{"id":"T9319","span":{"begin":38,"end":45},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    biosemtest

    {"project":"biosemtest","denotations":[{"id":"T9720","span":{"begin":94,"end":97},"obj":"UMLS/C0812225"},{"id":"T9719","span":{"begin":77,"end":80},"obj":"UMLS/C0812225"},{"id":"T9718","span":{"begin":94,"end":97},"obj":"UMLS/C1704742"},{"id":"T9717","span":{"begin":77,"end":80},"obj":"UMLS/C1704742"},{"id":"T9711","span":{"begin":173,"end":177},"obj":"UMLS/C0006556"},{"id":"T9710","span":{"begin":159,"end":171},"obj":"UMLS/C1442085"},{"id":"T9704","span":{"begin":144,"end":158},"obj":"UMLS/C1546933"},{"id":"T9697","span":{"begin":328,"end":335},"obj":"UMLS/C0072149"},{"id":"T9686","span":{"begin":199,"end":204},"obj":"UMLS/C0439810"},{"id":"T9685","span":{"begin":0,"end":5},"obj":"UMLS/C0439810"},{"id":"T9680","span":{"begin":243,"end":264},"obj":"UP/P21329"},{"id":"T9679","span":{"begin":67,"end":76},"obj":"UMLS/C0204727"},{"id":"T9664","span":{"begin":6,"end":14},"obj":"UMLS/C0007634"},{"id":"T9642","span":{"begin":144,"end":158},"obj":"UMLS/C1705426"},{"id":"T9635","span":{"begin":127,"end":136},"obj":"UMLS/C0680240"},{"id":"T9634","span":{"begin":243,"end":264},"obj":"UMLS/C0035379"},{"id":"T9627","span":{"begin":243,"end":264},"obj":"UP/P23070"},{"id":"T9626","span":{"begin":144,"end":158},"obj":"UMLS/C0947322"},{"id":"T9624","span":{"begin":67,"end":76},"obj":"UMLS/C1547129"},{"id":"T9613","span":{"begin":23,"end":32},"obj":"UMLS/C1555707"},{"id":"T9610","span":{"begin":46,"end":53},"obj":"UMLS/C0039194"},{"id":"T9590","span":{"begin":159,"end":171},"obj":"UMLS/C0039401"},{"id":"T9586","span":{"begin":243,"end":264},"obj":"UP/Q95SX7"},{"id":"T9576","span":{"begin":23,"end":32},"obj":"UMLS/C0185115"},{"id":"T9559","span":{"begin":159,"end":171},"obj":"UMLS/C0220931"},{"id":"T9558","span":{"begin":159,"end":171},"obj":"UMLS/C1292796"},{"id":"T9434","span":{"begin":67,"end":76},"obj":"UMLS/C0205409"},{"id":"T9431","span":{"begin":282,"end":291},"obj":"UMLS/C0454845"},{"id":"T9415","span":{"begin":205,"end":208},"obj":"UMLS/C1325819"},{"id":"T9414","span":{"begin":63,"end":66},"obj":"UMLS/C1325819"},{"id":"T9413","span":{"begin":221,"end":242},"obj":"UMLS/C0026626"},{"id":"T9412","span":{"begin":15,"end":18},"obj":"UMLS/C1325819"},{"id":"T9403","span":{"begin":350,"end":353},"obj":"UMLS/C0041703"},{"id":"T9402","span":{"begin":297,"end":300},"obj":"UMLS/C0041703"},{"id":"T9401","span":{"begin":121,"end":124},"obj":"UMLS/C0041703"},{"id":"T9397","span":{"begin":67,"end":76},"obj":"UMLS/C1608300"},{"id":"T9392","span":{"begin":67,"end":76},"obj":"UMLS/C1608299"},{"id":"T9388","span":{"begin":67,"end":76},"obj":"UMLS/C1548355"},{"id":"T9379","span":{"begin":243,"end":264},"obj":"UP/Q9NBX4"},{"id":"T9374","span":{"begin":137,"end":139},"obj":"UMLS/C0319022"},{"id":"T9363","span":{"begin":319,"end":326},"obj":"UMLS/C0206415"},{"id":"T9362","span":{"begin":205,"end":208},"obj":"UMLS/C0035668"},{"id":"T9361","span":{"begin":63,"end":66},"obj":"UMLS/C0035668"},{"id":"T9360","span":{"begin":15,"end":18},"obj":"UMLS/C0035668"},{"id":"T9359","span":{"begin":243,"end":264},"obj":"UP/P21328"},{"id":"T9358","span":{"begin":94,"end":97},"obj":"UMLS/C1705212"},{"id":"T9357","span":{"begin":77,"end":80},"obj":"UMLS/C1705212"},{"id":"T9356","span":{"begin":107,"end":115},"obj":"UMLS/C1217267"},{"id":"T9554","span":{"begin":6,"end":14},"obj":"UMLS/C0178539"},{"id":"T9545","span":{"begin":89,"end":93},"obj":"UMLS/C0445542"},{"id":"T9543","span":{"begin":243,"end":264},"obj":"UP/P23072"},{"id":"T9542","span":{"begin":199,"end":204},"obj":"UMLS/C0439175"},{"id":"T9541","span":{"begin":0,"end":5},"obj":"UMLS/C0439175"},{"id":"T9511","span":{"begin":23,"end":32},"obj":"UMLS/C1705851"},{"id":"T9510","span":{"begin":182,"end":193},"obj":"UMLS/C1883254"},{"id":"T9507","span":{"begin":319,"end":326},"obj":"UMLS/C1279901"},{"id":"T9499","span":{"begin":269,"end":280},"obj":"UMLS/C0205474"},{"id":"T9497","span":{"begin":144,"end":158},"obj":"UMLS/C1548599"},{"id":"T9496","span":{"begin":94,"end":97},"obj":"UMLS/C1690540"},{"id":"T9495","span":{"begin":77,"end":80},"obj":"UMLS/C1690540"},{"id":"T9486","span":{"begin":67,"end":76},"obj":"UMLS/C0220862"},{"id":"T9485","span":{"begin":94,"end":97},"obj":"UMLS/C1553450"},{"id":"T9483","span":{"begin":77,"end":80},"obj":"UMLS/C1553450"},{"id":"T9477","span":{"begin":319,"end":326},"obj":"UMLS/C0205435"},{"id":"T9473","span":{"begin":243,"end":264},"obj":"UP/P23071"},{"id":"T9441","span":{"begin":159,"end":171},"obj":"UMLS/C0302828"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    pmc-enju-pas

    {"project":"pmc-enju-pas","denotations":[{"id":"T9888","span":{"begin":353,"end":354},"obj":"-RRB-"},{"id":"T9887","span":{"begin":350,"end":353},"obj":"NNP"},{"id":"T9886","span":{"begin":348,"end":349},"obj":"-COMMA-"},{"id":"T9885","span":{"begin":346,"end":348},"obj":"NNP"},{"id":"T9884","span":{"begin":344,"end":345},"obj":"-COMMA-"},{"id":"T9883","span":{"begin":337,"end":344},"obj":"NNP"},{"id":"T9882","span":{"begin":335,"end":336},"obj":"-COMMA-"},{"id":"T9881","span":{"begin":328,"end":335},"obj":"NNP"},{"id":"T9880","span":{"begin":327,"end":328},"obj":"-LRB-"},{"id":"T9879","span":{"begin":319,"end":326},"obj":"NN"},{"id":"T9878","span":{"begin":316,"end":318},"obj":"CD"},{"id":"T9877","span":{"begin":315,"end":316},"obj":"-RRB-"},{"id":"T9876","span":{"begin":313,"end":315},"obj":"NN"},{"id":"T9875","span":{"begin":312,"end":313},"obj":"-LRB-"},{"id":"T9874","span":{"begin":306,"end":312},"obj":"NN"},{"id":"T9873","span":{"begin":302,"end":305},"obj":"CC"},{"id":"T9872","span":{"begin":300,"end":301},"obj":"-RRB-"},{"id":"T9871","span":{"begin":297,"end":300},"obj":"NNP"},{"id":"T9870","span":{"begin":295,"end":296},"obj":"-COMMA-"},{"id":"T9869","span":{"begin":293,"end":295},"obj":"NNP"},{"id":"T9868","span":{"begin":291,"end":292},"obj":"-COMMA-"},{"id":"T9867","span":{"begin":282,"end":291},"obj":"NNP"},{"id":"T9866","span":{"begin":280,"end":281},"obj":"-COMMA-"},{"id":"T9865","span":{"begin":269,"end":280},"obj":"NNP"},{"id":"T9864","span":{"begin":266,"end":268},"obj":"NNP"},{"id":"T9863","span":{"begin":265,"end":266},"obj":"-LRB-"},{"id":"T9862","span":{"begin":251,"end":264},"obj":"NN"},{"id":"T9861","span":{"begin":243,"end":250},"obj":"JJ"},{"id":"T9860","span":{"begin":237,"end":242},"obj":"NN"},{"id":"T9859","span":{"begin":228,"end":236},"obj":"NN"},{"id":"T9858","span":{"begin":221,"end":227},"obj":"JJ"},{"id":"T9857","span":{"begin":214,"end":220},"obj":"NNP"},{"id":"T9856","span":{"begin":209,"end":213},"obj":"IN"},{"id":"T9855","span":{"begin":205,"end":208},"obj":"NN"},{"id":"T9854","span":{"begin":199,"end":204},"obj":"JJ"},{"id":"T9853","span":{"begin":194,"end":198},"obj":"IN"},{"id":"T9852","span":{"begin":182,"end":193},"obj":"VB"},{"id":"T9851","span":{"begin":178,"end":181},"obj":"VB"},{"id":"T9850","span":{"begin":173,"end":177},"obj":"NN"},{"id":"T9849","span":{"begin":159,"end":172},"obj":"NN"},{"id":"T9848","span":{"begin":156,"end":158},"obj":"POS"},{"id":"T9847","span":{"begin":144,"end":156},"obj":"NN"},{"id":"T9846","span":{"begin":140,"end":143},"obj":"DT"},{"id":"T9845","span":{"begin":137,"end":139},"obj":"TO"},{"id":"T9844","span":{"begin":127,"end":136},"obj":"VB"},{"id":"T9843","span":{"begin":125,"end":126},"obj":"-COMMA-"},{"id":"T9842","span":{"begin":124,"end":125},"obj":"-RRB-"},{"id":"T9841","span":{"begin":121,"end":124},"obj":"NNP"},{"id":"T9840","span":{"begin":119,"end":120},"obj":"-COMMA-"},{"id":"T9839","span":{"begin":117,"end":119},"obj":"NNP"},{"id":"T9838","span":{"begin":115,"end":116},"obj":"-COMMA-"},{"id":"T9837","span":{"begin":107,"end":115},"obj":"NNP"},{"id":"T9836","span":{"begin":105,"end":106},"obj":"-COMMA-"},{"id":"T9835","span":{"begin":99,"end":105},"obj":"NNP"},{"id":"T9834","span":{"begin":97,"end":98},"obj":"-COLON-"},{"id":"T9833","span":{"begin":94,"end":97},"obj":"NN"},{"id":"T9832","span":{"begin":89,"end":93},"obj":"NNP"},{"id":"T9831","span":{"begin":82,"end":88},"obj":"NNP"},{"id":"T9830","span":{"begin":81,"end":82},"obj":"-LRB-"},{"id":"T9829","span":{"begin":77,"end":80},"obj":"NN"},{"id":"T9828","span":{"begin":67,"end":76},"obj":"NN"},{"id":"T9827","span":{"begin":63,"end":66},"obj":"NN"},{"id":"T9826","span":{"begin":60,"end":62},"obj":"DT"},{"id":"T9825","span":{"begin":54,"end":59},"obj":"VB"},{"id":"T9824","span":{"begin":48,"end":53},"obj":"NN"},{"id":"T9823","span":{"begin":46,"end":47},"obj":"NN"},{"id":"T9822","span":{"begin":41,"end":45},"obj":"JJ"},{"id":"T9821","span":{"begin":38,"end":40},"obj":"NN"},{"id":"T9820","span":{"begin":33,"end":37},"obj":"IN"},{"id":"T9819","span":{"begin":23,"end":32},"obj":"VB"},{"id":"T9818","span":{"begin":19,"end":22},"obj":"VB"},{"id":"T9817","span":{"begin":15,"end":18},"obj":"NN"},{"id":"T9816","span":{"begin":6,"end":14},"obj":"JJ"},{"id":"T9815","span":{"begin":0,"end":5},"obj":"JJ"}],"relations":[{"id":"R4796","pred":"arg1Of","subj":"T9817","obj":"T9815"},{"id":"R4797","pred":"arg1Of","subj":"T9817","obj":"T9816"},{"id":"R4798","pred":"arg1Of","subj":"T9817","obj":"T9818"},{"id":"R4799","pred":"arg2Of","subj":"T9817","obj":"T9819"},{"id":"R4800","pred":"arg2Of","subj":"T9819","obj":"T9818"},{"id":"R4801","pred":"arg1Of","subj":"T9819","obj":"T9820"},{"id":"R4802","pred":"arg2Of","subj":"T9824","obj":"T9820"},{"id":"R4803","pred":"arg1Of","subj":"T9824","obj":"T9821"},{"id":"R4804","pred":"arg1Of","subj":"T9824","obj":"T9822"},{"id":"R4805","pred":"arg1Of","subj":"T9824","obj":"T9823"},{"id":"R4806","pred":"arg1Of","subj":"T9824","obj":"T9825"},{"id":"R4807","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cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    bionlp-st-ge-2016-test-proteins

    {"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T9750","span":{"begin":41,"end":44},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    bionlp-st-ge-2016-uniprot

    {"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T9791","span":{"begin":41,"end":44},"obj":"http://www.uniprot.org/uniprot/P01730"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    GO-BP

    {"project":"GO-BP","denotations":[{"id":"T9735","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0034062"},{"id":"T9734","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0003968"},{"id":"T9733","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0003899"},{"id":"T9732","span":{"begin":117,"end":119},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T9731","span":{"begin":6,"end":14},"obj":"http://purl.obolibrary.org/obo/GO_0007349"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    GO-CC

    {"project":"GO-CC","denotations":[{"id":"T9765","span":{"begin":48,"end":53},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    GO-MF

    {"project":"GO-MF","denotations":[{"id":"T9764","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0034062"},{"id":"T9763","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0003968"},{"id":"T9762","span":{"begin":251,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0003899"},{"id":"T9761","span":{"begin":117,"end":119},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    sentences

    {"project":"sentences","denotations":[{"id":"T9724","span":{"begin":0,"end":355},"obj":"Sentence"},{"id":"T70","span":{"begin":0,"end":355},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    simple1

    {"project":"simple1","denotations":[{"id":"T9798","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    BioNLP16_DUT

    {"project":"BioNLP16_DUT","denotations":[{"id":"T10536","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    BioNLP16_Messiy

    {"project":"BioNLP16_Messiy","denotations":[{"id":"T10113","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    DLUT931

    {"project":"DLUT931","denotations":[{"id":"T10469","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    bionlp-st-ge-2016-test-ihmc

    {"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T10533","span":{"begin":312,"end":318},"obj":"Entity"},{"id":"T10529","span":{"begin":199,"end":208},"obj":"Protein"},{"id":"T10520","span":{"begin":63,"end":66},"obj":"Protein"},{"id":"T10514","span":{"begin":38,"end":80},"obj":"Entity"},{"id":"T10510","span":{"begin":214,"end":264},"obj":"Protein"},{"id":"T10509","span":{"begin":38,"end":40},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    bionlp-st-ge-2016-spacy-parsed

    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cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    bionlp-st-ge-2016-test-tees

    {"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T9777","span":{"begin":243,"end":264},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    test3

    {"project":"test3","denotations":[{"id":"T9342","span":{"begin":41,"end":44},"obj":"Protein"},{"id":"T9331","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}

    testone

    {"project":"testone","denotations":[{"id":"T9306","span":{"begin":41,"end":44},"obj":"Protein"}],"text":"Total cellular RNA was extracted from PB CD4+ T cells using an RNA isolation kit (RNeasy Mini kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. cDNA was synthesized from total RNA with Molony murine leukemia virus reverse transcriptase (US Biochemical, Cleveland, OH, USA) and oligo-(dT)15 primers (Promega, Madison, WI, USA)."}