PMC:1064854 / 28714-29835 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1064854","sourcedb":"PMC","sourceid":"1064854","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1064854","text":"Figure 6 PGC-1α−/− Mice Exhibit an Abnormal Thermogenic Response\n(A) PGC-1α+/+ (n = 15) and PGC-1α−/− (n = 21) mice aged 28–37 d were subjected to cold (4 °C). Core rectal temperature was monitored over a 5-h period. The change in core temperature ± SEM is shown in the graph (left) as a function of time. * p \u003c 0.05.\n(B) Representative Northern blot analysis (blot and gel at top) performed with RNA isolated from BAT to detect UCP-1 transcript at baseline (RT) and after 5 h of exposure to cold (4 °C) (UCP1). Ethidium bromide (Eth Br) staining of ribosomal RNA is shown as a control. Quantitative real-time RT-PCR for UCP-1 transcript is shown on the graph at the bottom. The values represent mean arbitrary units normalized to a 36B4 transcript (control).\n(C) Altered response to β3-adrenergic agonist. To evaluate the oxygen consumption (VO2) in response to the stimulation of BAT uncoupled respiration, the β3-adrenergic agonist BRL 37344 was administered to littermate PGC-1α+/+ (n = 5) and PGC-1α−/− (n = 5) female mice followed by measurement of VO2 by indirect calorimetry. Mean ± SEM VO2 is shown. * p \u003c 0.05.","divisions":[{"label":"title","span":{"begin":10,"end":65}},{"label":"p","span":{"begin":66,"end":318}},{"label":"p","span":{"begin":319,"end":760}}],"tracks":[]}