PMC:103662 / 18881-19601 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/103662","sourcedb":"PMC","sourceid":"103662","source_url":"https://www.ncbi.nlm.nih.gov/pmc/103662","text":"Béjà et al. reported a strong positive 580-nm transient absorbance increase in suspensions of membranes prepared from E. coli expressing pR [1]. This positive absorbance difference is indicative of O intermediate formation. We looked for its presence in partially-purified samples reconstituted in DHPC in the pH range 6–11. (Data are shown only at pH 9.5; see Fig. 4). Our observations at pH 9.5 do not show any evidence of O intermediate formation (Fig. 4). Only at lower pH values was a clear positive 580-nm absorption change observed (data not shown). This is in agreement with results on bR, for which O formation is also enhanced at lower pH values, and becomes small or nearly unobservable in the alkaline range.","tracks":[]}