PMC:102758 / 10787-11291 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/102758","sourcedb":"PMC","sourceid":"102758","source_url":"https://www.ncbi.nlm.nih.gov/pmc/102758","text":"PTP-1B is a substrate for 124-v-Mos in vitro. In vitro Mos kinase assays, using purified PTP-1B as a substrate, were resolved using 10% SDS-PAGE and the autoradiograph is shown in 4A. Immunoprecipitates of Sf9 cells expressing the kinase-inactive 124-v-MosK121R variant or PTP-1B alone were included as controls (A,B). A parallel kinase assay was blotted on nylon-membrane and PTP-1B was detected (B) using the PTP-1B-specific antiserum FG6 [29], arrowheads indicate the position of 124-v-Mos and PTP-1B.","tracks":[{"project":"2_test","denotations":[{"id":"12022922-2554568-8102872","span":{"begin":442,"end":444},"obj":"2554568"}],"attributes":[{"subj":"12022922-2554568-8102872","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#b8ec93","default":true}]}]}}