PMC:101493 / 13380-14242 JSONTXT

{"project":"2_test","denotations":[{"id":"11934354-9415894-8571156","span":{"begin":111,"end":112},"obj":"9415894"}],"text":"The DefH9 gene is expressed specifically in the placenta and ovules during early phases of flower development [6]. To determine whether expression of the DefH9-iaaM gene also occurs during later stages of fruit growth and whether it is influenced by pollen fertilization, mRNA from transgenic flowers and fruits obtained either from emasculated or hand pollinated flowers was analyzed by RT-PCR at different stages of development, until the fruit attained a length of 28 cm. An amplicon of 161 bp corresponding to the spliced DefH9-iaaM mRNA was detected in all stages analyzed (Fig. 3, lanes 2–6). Thus, the presence of the mRNA of the DefH9-iaaM gene and consequently its action is most likely not limited to early stages of flower and fruit development. Pollination did not affect the steady state level of DefH9-iaaM mRNA (Fig. 3, compare lane 5 and lane 6)."}