PMC:101407 / 8107-9259 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/101407","sourcedb":"PMC","sourceid":"101407","source_url":"https://www.ncbi.nlm.nih.gov/pmc/101407","text":"Cytotoxic effects of ALA in Human Cancer Cells\nHuman hepatocarcinoma cells were treated with various concentrations (0.5–5 mM) of ALA and the viability of cells was determined (Figure 1). ALA was toxic to both hepatocarcinoma cell lines. In HEP G2 cells, cytotoxicity occurred after exposure to 0.5 mM ALA for 24 h (growth inhibition: 30%) and the highest growth inhibition (70%) was observed with the highest ALA concentration (5 mM). HEP 3B cells were more resistant to ALA than HEP G2 cells, exhibiting only 12% growth inhibition after exposure to 0.5 mM ALA for 24 h and a 40% growth inhibition with 5 mM ALA. The strange shape of the viability curves obtained in both cases, with a restoration of control viability with 2 mM ALA, cannot be explained.\nFigure 1 Cytotoxic effects of ALA. Human hepatocarcinoma cell lines (HEP G2 (circles) HEP 3B (squares)) were grown in the absence or in the presence of increasing ALA concentrations (0.5 to 5 mM) and the viability of cells was determined by the MTT assay. Viability was expressed in terms of population growth, as percent of an untreated control population with standard error of the mean (SEM).","divisions":[{"label":"title","span":{"begin":0,"end":46}},{"label":"p","span":{"begin":47,"end":755}},{"label":"label","span":{"begin":756,"end":764}}],"tracks":[]}