PMC:101403 / 20497-21179 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/101403","sourcedb":"PMC","sourceid":"101403","source_url":"https://www.ncbi.nlm.nih.gov/pmc/101403","text":"Potential substrates were screened by measuring the Pi released after nucleotide hydrolysis in presence of inorganic pyrophosphatase or alkaline phosphatase [9]. The standard assay (200 μl) for phosphodiester substrates was incubated at 37°C for 30 min and contained 50 mM l,3-bis [tris(hydroxymethyl)-methylamino]propane-HCl (BisTrisPropane-HCl), pH 8.0, 5 mM MgCl2, 1 mM DTT, 0.5 mM substrate, 0.1 μg of Trx-Y87G2A.14 fusion protein and 0.5 μg (1 unit) alkaline phosphatase. Assays with phosphomonoester substrates were as above, except 0.5 μg (100 mU) inorganic pyrophosphatase was used instead of alkaline phosphatase. The Pi released in each case was measured colorimetrically.","tracks":[{"project":"2_test","denotations":[{"id":"11943069-10567213-8112341","span":{"begin":158,"end":159},"obj":"10567213"}],"attributes":[{"subj":"11943069-10567213-8112341","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#af93ec","default":true}]}]}}