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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/101390","sourcedb":"PMC","sourceid":"101390","source_url":"https://www.ncbi.nlm.nih.gov/pmc/101390","text":"Enzyme assay\nCholesterol oxidase activity was assayed by a modification of the method of Allain et al. [1] as described previously [9]. One unit of activity was defined as the amount of enzyme that converts 1 μmole of cholesterol/min at 37°C. All samples were diluted before enzyme assay to a final Triton X-114 concentration of 0.1% to avoid detergent interference with the assay [16].","divisions":[{"label":"title","span":{"begin":0,"end":12}}],"tracks":[{"project":"2_test","denotations":[{"id":"11914155-4818200-8561991","span":{"begin":104,"end":105},"obj":"4818200"},{"id":"11914155-9210347-8561992","span":{"begin":132,"end":133},"obj":"9210347"},{"id":"11914155-1368208-8561993","span":{"begin":382,"end":384},"obj":"1368208"}],"attributes":[{"subj":"11914155-4818200-8561991","pred":"source","obj":"2_test"},{"subj":"11914155-9210347-8561992","pred":"source","obj":"2_test"},{"subj":"11914155-1368208-8561993","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#a0ec93","default":true}]}]}}