PMC:101390 / 15453-16131 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/101390","sourcedb":"PMC","sourceid":"101390","source_url":"https://www.ncbi.nlm.nih.gov/pmc/101390","text":"An exceptional result was obtained when performing COX purification from the culture broth supplemented with a 6% w/v Triton X-114. The total activity recovered after phase separation was ca. 3.5-fold that measured in the broth before phase separation. This result suggests that soluble COX produced by the culture is not fully active and that it can be activated by a treatment with 6% Triton X-114 but not with 4% or less. Further increase of Triton X-114 concentration results in no improvement with respect to 6% (results not shown). This phenomenon was not observed with COX extracted from cells, therefore the enzyme most likely exists in a fully active form in the cells.","tracks":[]}