PMC:100321 / 3682-4777 JSONTXT

{"project":"2_test","denotations":[{"id":"11882251-9265640-8190347","span":{"begin":137,"end":138},"obj":"9265640"},{"id":"11882251-10090733-8190348","span":{"begin":139,"end":140},"obj":"10090733"},{"id":"11882251-10090733-8190349","span":{"begin":448,"end":449},"obj":"10090733"},{"id":"11882251-10090733-8190350","span":{"begin":750,"end":751},"obj":"10090733"},{"id":"11882251-1331987-8190351","span":{"begin":752,"end":753},"obj":"1331987"}],"text":"Extensive studies exist for assessing the thermodynamic consequences of internal mismatches in short oligo-nucleotides (see fro example [6,7]). These show that there are no simple rules and that the exact influence on the stability of a hybrid depends on the nature of the mismatch, as well as its flanking nucleotides. For example, mismatches including a G (i.e. G-G, G-T and G-A) tend to be less destabilizing than the other types of mismatches [7], although this can not directly be predicted from steric considerations. Comparable systematic studies on the relative influence of the position of the mismatch within the oligonucleotide do not exist yet, although it is clear that the influence is lower at the ends than in more central positions [7,9]. Preliminary evidence with an oligo-dT stretch harboring A mismatches along the sequence suggests that the position dependence could be a continuous function [8]. We have therefore decided to use an ad hoc approach for the stability calculation that is mainly designed to discriminate against sequences with more central mismatch positions."}