CORD-19:e847d433e7424152a9846673209cb73027415ecd / 404456-405343 JSONTXT

{"project":"CORD-19-Sentences","denotations":[{"id":"TextSentencer_T3713","span":{"begin":0,"end":887},"obj":"Sentence"},{"id":"T97653","span":{"begin":0,"end":887},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"NanoChip Ò 400 system and its accompanying electronic microchip in four distinct steps: (1) biotinylated capture oligonucleotides complementary to the seven respiratory viruses as well as an optional internal control were electronically targeted to specific sites across the 400-site cartridge; (2) a dilution of the RT-PCR reaction was electronically biased to a set of pads comprising the full set of capture oligonucleotides, allowing any generated amplicon to hybridise to its complementary capture sequence; (3) each additional RT-PCR reaction was sequentially biased to a unique set of sites across the cartridge; (4) passive hybridisation using a set of bi-functional discriminator oligonucleotides (half of the molecule binds a specific amplicon and half binds to a reporter molecule) and 2 fluorescently-labelled reporter oligonucleotides allowed for detection of the amplicons."}

{"project":"CORD-19_Custom_license_subset","denotations":[{"id":"T175","span":{"begin":0,"end":887},"obj":"Sentence"}],"text":"NanoChip Ò 400 system and its accompanying electronic microchip in four distinct steps: (1) biotinylated capture oligonucleotides complementary to the seven respiratory viruses as well as an optional internal control were electronically targeted to specific sites across the 400-site cartridge; (2) a dilution of the RT-PCR reaction was electronically biased to a set of pads comprising the full set of capture oligonucleotides, allowing any generated amplicon to hybridise to its complementary capture sequence; (3) each additional RT-PCR reaction was sequentially biased to a unique set of sites across the cartridge; (4) passive hybridisation using a set of bi-functional discriminator oligonucleotides (half of the molecule binds a specific amplicon and half binds to a reporter molecule) and 2 fluorescently-labelled reporter oligonucleotides allowed for detection of the amplicons."}