CORD-19:ceba28d8304f3df8001164f8edeef545154289f6 / 43637-43896 JSONTXT

{"project":"CORD-19-Sentences","denotations":[{"id":"TextSentencer_T245","span":{"begin":0,"end":259},"obj":"Sentence"},{"id":"TextSentencer_T245","span":{"begin":0,"end":259},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"For detection of VSV gRNA, total brain RNA was isolated using the Maxwell 16 simplyRNA Tissue kit. cDNA was prepared as above and SYBR green qPCR was carried out with primers amplifying VSV gRNA (fwd 5'-ATGTCACTGCAAGGCCTAAGA-3', rev 5'-ATCTCTCCTACCGCCTGATCC)."}

{"project":"CORD-19-PD-UBERON","denotations":[{"id":"T129","span":{"begin":33,"end":38},"obj":"Body_part"},{"id":"T130","span":{"begin":87,"end":93},"obj":"Body_part"}],"attributes":[{"id":"A129","pred":"uberon_id","subj":"T129","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"A130","pred":"uberon_id","subj":"T130","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"}],"text":"For detection of VSV gRNA, total brain RNA was isolated using the Maxwell 16 simplyRNA Tissue kit. cDNA was prepared as above and SYBR green qPCR was carried out with primers amplifying VSV gRNA (fwd 5'-ATGTCACTGCAAGGCCTAAGA-3', rev 5'-ATCTCTCCTACCGCCTGATCC)."}