CORD-19:b75e7f3f923023185daed6f29e5beca4eddfc109 / 47913-48160 JSONTXT

{"project":"CORD-19-Sentences","denotations":[{"id":"TextSentencer_T289","span":{"begin":0,"end":247},"obj":"Sentence"},{"id":"T82215","span":{"begin":0,"end":247},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Briefly, washed beads were resuspended in 100 l of distilled water and then reduced in 2 mM dithiothreitol (Sigma-Aldrich) at 56°C for 30 min, followed by alkylation in 5 mM iodoacetamide (Sigma-Aldrich) for 30 min at room temperature in the dark."}