CORD-19:58c67994f47dc6c8b552cff7de2892719259ec52 / 14703-15010 JSONTXT

{"project":"CORD-19-Sentences","denotations":[{"id":"TextSentencer_T93","span":{"begin":0,"end":307},"obj":"Sentence"},{"id":"TextSentencer_T93","span":{"begin":0,"end":307},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To further quantify the relative contribution of positively charged residues to oligonucleotide binding by the SARS-CoV NP CTD, we produced double mutants targeting K257/K258 and measured the effect of the mutations on the apparent dissociation constant (K d ) with fluorescently labeled dT 20 through EMSA."}

{"project":"CORD-19_Custom_license_subset","denotations":[{"id":"T93","span":{"begin":0,"end":307},"obj":"Sentence"}],"text":"To further quantify the relative contribution of positively charged residues to oligonucleotide binding by the SARS-CoV NP CTD, we produced double mutants targeting K257/K258 and measured the effect of the mutations on the apparent dissociation constant (K d ) with fluorescently labeled dT 20 through EMSA."}

{"project":"CORD-19-PD-MONDO","denotations":[{"id":"T76","span":{"begin":111,"end":119},"obj":"Disease"},{"id":"T77","span":{"begin":111,"end":115},"obj":"Disease"},{"id":"T71771","span":{"begin":111,"end":119},"obj":"Disease"},{"id":"T47812","span":{"begin":111,"end":115},"obj":"Disease"}],"attributes":[{"id":"A76","pred":"mondo_id","subj":"T76","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A77","pred":"mondo_id","subj":"T77","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A22590","pred":"mondo_id","subj":"T71771","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A38983","pred":"mondo_id","subj":"T47812","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"To further quantify the relative contribution of positively charged residues to oligonucleotide binding by the SARS-CoV NP CTD, we produced double mutants targeting K257/K258 and measured the effect of the mutations on the apparent dissociation constant (K d ) with fluorescently labeled dT 20 through EMSA."}