CORD-19:4aa092956fea94f9487d129b4a05a8704b73d0fc / 25985-26260 JSONTXT

{"project":"CORD-19-Sentences","denotations":[{"id":"TextSentencer_T183","span":{"begin":0,"end":275},"obj":"Sentence"},{"id":"TextSentencer_T183","span":{"begin":0,"end":275},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"At 24 h after the transfection, the cell lysates were prepared in lysis buffer containing 1.0% (vol/vol) Nonidet P40, 20 mM Tris-HCl, pH 8.0, 10%(vol/vol) glycerol, 150 mM NaCl, 0.2 mM Na3VO4, 1 mM NaF, 0.1 mM sodium pyrophosphate and a protease inhibitor 'cocktail' (Roche)."}