CORD-19:12f1cc78e99a0b82f3225d10abe9a5649f1b33fc / 6322-6638 JSONTXT

{"project":"CORD-19_Custom_license_subset","denotations":[{"id":"T36","span":{"begin":0,"end":316},"obj":"Sentence"}],"text":"The recombinant N protein was expressed by the transformation of BL21(DE3) single competent cells (Novagen, Inc.) by the plasmid and was purified by use of the Ni 2ϩ -loaded HiTrap chelating system (Amersham Biosciences, Piscataway, NJ) according to the manufacturer's instructions and as described previously (16) ."}

{"project":"CORD-19-Sentences","denotations":[{"id":"TextSentencer_T36","span":{"begin":0,"end":316},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The recombinant N protein was expressed by the transformation of BL21(DE3) single competent cells (Novagen, Inc.) by the plasmid and was purified by use of the Ni 2ϩ -loaded HiTrap chelating system (Amersham Biosciences, Piscataway, NJ) according to the manufacturer's instructions and as described previously (16) ."}