BB-rel@ldeleger:BB-rel-24722168 / 177-2157 JSONTXT

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bionlp-ost-19-BB-rel-test

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Id Subject Object Predicate Lexical cue
T2 0-1980 Paragraph denotes This study aims to establish a human gut microbiota (HGM) transplanted gnotobiotic (Gn) pig model of human rotavirus (HRV) infection and diarrhea, and to verify the dose-effects of probiotics on HRV vaccine-induced immune responses. Our previous studies using the Gn pig model found that probiotics dose-dependently regulated both T cell and B cell immune responses induced by rotavirus vaccines. We generated the HGM transplanted neonatal Gn pigs through daily feeding of neonatal human fecal suspension to germ-free pigs for 3 days starting at 12 hours after birth. We found that attenuated HRV (AttHRV) vaccination conferred similar overall protection against rotavirus diarrhea and virus shedding in Gn pigs and HGM transplanted Gn pigs. HGM promoted the development of the neonatal immune system, as evidenced by the significantly enhanced IFN-γ producing T cell responses and reduction of regulatory T cells and their cytokine production in the AttHRV-vaccinated pigs. The higher dose Lactobacillus rhamnosus GG (LGG) feeding (14 doses, up to 109 colony-forming-unit [CFU]/dose) effectively increased the LGG counts in the HGM Gn pig intestinal contents and significantly enhanced HRV-specific IFN-γ producing T cell responses to the AttHRV vaccine. Lower dose LGG (9 doses, up to 106 CFU/dose) was ineffective. Neither doses of LGG significantly improved the protection rate, HRV-specific IgA and IgG antibody titers in serum, or IgA antibody titers in intestinal contents compared to the AttHRV vaccine alone, suggesting that an even higher dose of LGG is needed to overcome the influence of the microbiota to achieve the immunostimulatory effect in the HGM pigs. This study demonstrated that HGM Gn pig is an applicable animal model for studying immune responses to rotavirus vaccines and can be used for studying interventions (i.e., probiotics and prebiotics) that may enhance the immunogenicity and protective efficacy of vaccines through improving the gut microbiota.
T12 31-97 Habitat denotes human gut microbiota (HGM) transplanted gnotobiotic (Gn) pig model
T13 31-57 Habitat denotes human gut microbiota (HGM)
T10 31-40 Habitat denotes human gut
T11 31-36 Habitat denotes human
T14 53-56 Habitat denotes HGM
T16 101-116 Microorganism denotes human rotavirus
T15 101-106 Habitat denotes human
T17 118-121 Microorganism denotes HRV
T18 181-191 Phenotype denotes probiotics
T19 195-206 Habitat denotes HRV vaccine
T20 195-198 Microorganism denotes HRV
T21 264-276 Habitat denotes Gn pig model
T22 288-298 Phenotype denotes probiotics
T23 331-337 Habitat denotes T cell
T24 342-348 Habitat denotes B cell
T25 377-395 Habitat denotes rotavirus vaccines
T26 377-386 Microorganism denotes rotavirus
T27 414-447 Habitat denotes HGM transplanted neonatal Gn pigs
T28 414-417 Habitat denotes HGM
T30 473-504 Habitat denotes neonatal human fecal suspension
T29 473-493 Habitat denotes neonatal human fecal
T31 473-487 Habitat denotes neonatal human
T32 508-522 Habitat denotes germ-free pigs
T33 593-596 Microorganism denotes HRV
T34 598-604 Microorganism denotes AttHRV
T35 663-672 Microorganism denotes rotavirus
T36 704-711 Habitat denotes Gn pigs
T38 716-740 Habitat denotes HGM transplanted Gn pigs
T37 716-719 Habitat denotes HGM
T39 742-745 Habitat denotes HGM
T40 778-800 Habitat denotes neonatal immune system
T41 861-867 Habitat denotes T cell
T42 906-913 Habitat denotes T cells
T43 951-973 Habitat denotes AttHRV-vaccinated pigs
T44 951-957 Microorganism denotes AttHRV
T45 991-1017 Microorganism denotes Lactobacillus rhamnosus GG
T46 1019-1022 Microorganism denotes LGG
T47 1111-1114 Microorganism denotes LGG
T48 1129-1159 Habitat denotes HGM Gn pig intestinal contents
T49 1129-1150 Habitat denotes HGM Gn pig intestinal
T50 1129-1139 Habitat denotes HGM Gn pig
T51 1129-1132 Habitat denotes HGM
T52 1187-1190 Microorganism denotes HRV
T53 1216-1222 Habitat denotes T cell
T54 1240-1254 Habitat denotes AttHRV vaccine
T55 1240-1246 Microorganism denotes AttHRV
T56 1267-1270 Microorganism denotes LGG
T57 1335-1338 Microorganism denotes LGG
T58 1383-1386 Microorganism denotes HRV
T59 1427-1432 Habitat denotes serum
T60 1460-1479 Habitat denotes intestinal contents
T61 1460-1470 Habitat denotes intestinal
T62 1496-1510 Habitat denotes AttHRV vaccine
T63 1496-1502 Microorganism denotes AttHRV
T64 1557-1560 Microorganism denotes LGG
T65 1604-1614 Habitat denotes microbiota
T66 1662-1670 Habitat denotes HGM pigs
T67 1662-1665 Habitat denotes HGM
T69 1701-1711 Habitat denotes HGM Gn pig
T68 1701-1704 Habitat denotes HGM
T70 1729-1741 Habitat denotes animal model
T71 1775-1793 Habitat denotes rotavirus vaccines
T72 1775-1784 Microorganism denotes rotavirus
T73 1844-1854 Phenotype denotes probiotics
T74 1934-1942 Habitat denotes vaccines
T76 1965-1979 Habitat denotes gut microbiota
T75 1965-1968 Habitat denotes gut