BB-rel@ldeleger:BB-rel-24722168 JSONTXT

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bionlp-ost-19-BB-rel-test

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Id Subject Object Predicate Lexical cue
T1 0-176 Title denotes Probiotic Lactobacillus rhamnosus GG enhanced Th1 cellular immunity but did not affect antibody responses in a human gut microbiota transplanted neonatal gnotobiotic pig model.
T3 0-9 Phenotype denotes Probiotic
T4 10-36 Microorganism denotes Lactobacillus rhamnosus GG
T5 46-58 Habitat denotes Th1 cellular
T7 111-175 Habitat denotes human gut microbiota transplanted neonatal gnotobiotic pig model
T9 111-131 Habitat denotes human gut microbiota
T8 111-120 Habitat denotes human gut
T6 111-116 Habitat denotes human
T2 177-2157 Paragraph denotes This study aims to establish a human gut microbiota (HGM) transplanted gnotobiotic (Gn) pig model of human rotavirus (HRV) infection and diarrhea, and to verify the dose-effects of probiotics on HRV vaccine-induced immune responses. Our previous studies using the Gn pig model found that probiotics dose-dependently regulated both T cell and B cell immune responses induced by rotavirus vaccines. We generated the HGM transplanted neonatal Gn pigs through daily feeding of neonatal human fecal suspension to germ-free pigs for 3 days starting at 12 hours after birth. We found that attenuated HRV (AttHRV) vaccination conferred similar overall protection against rotavirus diarrhea and virus shedding in Gn pigs and HGM transplanted Gn pigs. HGM promoted the development of the neonatal immune system, as evidenced by the significantly enhanced IFN-γ producing T cell responses and reduction of regulatory T cells and their cytokine production in the AttHRV-vaccinated pigs. The higher dose Lactobacillus rhamnosus GG (LGG) feeding (14 doses, up to 109 colony-forming-unit [CFU]/dose) effectively increased the LGG counts in the HGM Gn pig intestinal contents and significantly enhanced HRV-specific IFN-γ producing T cell responses to the AttHRV vaccine. Lower dose LGG (9 doses, up to 106 CFU/dose) was ineffective. Neither doses of LGG significantly improved the protection rate, HRV-specific IgA and IgG antibody titers in serum, or IgA antibody titers in intestinal contents compared to the AttHRV vaccine alone, suggesting that an even higher dose of LGG is needed to overcome the influence of the microbiota to achieve the immunostimulatory effect in the HGM pigs. This study demonstrated that HGM Gn pig is an applicable animal model for studying immune responses to rotavirus vaccines and can be used for studying interventions (i.e., probiotics and prebiotics) that may enhance the immunogenicity and protective efficacy of vaccines through improving the gut microbiota.
T12 208-274 Habitat denotes human gut microbiota (HGM) transplanted gnotobiotic (Gn) pig model
T13 208-234 Habitat denotes human gut microbiota (HGM)
T10 208-217 Habitat denotes human gut
T11 208-213 Habitat denotes human
T14 230-233 Habitat denotes HGM
T16 278-293 Microorganism denotes human rotavirus
T15 278-283 Habitat denotes human
T17 295-298 Microorganism denotes HRV
T18 358-368 Phenotype denotes probiotics
T19 372-383 Habitat denotes HRV vaccine
T20 372-375 Microorganism denotes HRV
T21 441-453 Habitat denotes Gn pig model
T22 465-475 Phenotype denotes probiotics
T23 508-514 Habitat denotes T cell
T24 519-525 Habitat denotes B cell
T25 554-572 Habitat denotes rotavirus vaccines
T26 554-563 Microorganism denotes rotavirus
T27 591-624 Habitat denotes HGM transplanted neonatal Gn pigs
T28 591-594 Habitat denotes HGM
T30 650-681 Habitat denotes neonatal human fecal suspension
T29 650-670 Habitat denotes neonatal human fecal
T31 650-664 Habitat denotes neonatal human
T32 685-699 Habitat denotes germ-free pigs
T33 770-773 Microorganism denotes HRV
T34 775-781 Microorganism denotes AttHRV
T35 840-849 Microorganism denotes rotavirus
T36 881-888 Habitat denotes Gn pigs
T38 893-917 Habitat denotes HGM transplanted Gn pigs
T37 893-896 Habitat denotes HGM
T39 919-922 Habitat denotes HGM
T40 955-977 Habitat denotes neonatal immune system
T41 1038-1044 Habitat denotes T cell
T42 1083-1090 Habitat denotes T cells
T43 1128-1150 Habitat denotes AttHRV-vaccinated pigs
T44 1128-1134 Microorganism denotes AttHRV
T45 1168-1194 Microorganism denotes Lactobacillus rhamnosus GG
T46 1196-1199 Microorganism denotes LGG
T47 1288-1291 Microorganism denotes LGG
T48 1306-1336 Habitat denotes HGM Gn pig intestinal contents
T49 1306-1327 Habitat denotes HGM Gn pig intestinal
T50 1306-1316 Habitat denotes HGM Gn pig
T51 1306-1309 Habitat denotes HGM
T52 1364-1367 Microorganism denotes HRV
T53 1393-1399 Habitat denotes T cell
T54 1417-1431 Habitat denotes AttHRV vaccine
T55 1417-1423 Microorganism denotes AttHRV
T56 1444-1447 Microorganism denotes LGG
T57 1512-1515 Microorganism denotes LGG
T58 1560-1563 Microorganism denotes HRV
T59 1604-1609 Habitat denotes serum
T60 1637-1656 Habitat denotes intestinal contents
T61 1637-1647 Habitat denotes intestinal
T62 1673-1687 Habitat denotes AttHRV vaccine
T63 1673-1679 Microorganism denotes AttHRV
T64 1734-1737 Microorganism denotes LGG
T65 1781-1791 Habitat denotes microbiota
T66 1839-1847 Habitat denotes HGM pigs
T67 1839-1842 Habitat denotes HGM
T69 1878-1888 Habitat denotes HGM Gn pig
T68 1878-1881 Habitat denotes HGM
T70 1906-1918 Habitat denotes animal model
T71 1952-1970 Habitat denotes rotavirus vaccines
T72 1952-1961 Microorganism denotes rotavirus
T73 2021-2031 Phenotype denotes probiotics
T74 2111-2119 Habitat denotes vaccines
T76 2142-2156 Habitat denotes gut microbiota
T75 2142-2145 Habitat denotes gut