BB-rel@ldeleger:BB-rel-23902156 JSONTXT

{"project":"bionlp-ost-19-BB-rel-dev","denotations":[{"id":"T1","span":{"begin":0,"end":90},"obj":"Title"},{"id":"T3","span":{"begin":68,"end":89},"obj":"Microorganism"},{"id":"T2","span":{"begin":91,"end":1393},"obj":"Paragraph"},{"id":"T4","span":{"begin":129,"end":155},"obj":"Microorganism"},{"id":"T5","span":{"begin":258,"end":279},"obj":"Microorganism"},{"id":"T6","span":{"begin":340,"end":352},"obj":"Microorganism"},{"id":"T7","span":{"begin":479,"end":495},"obj":"Microorganism"},{"id":"T9","span":{"begin":1101,"end":1134},"obj":"Habitat"},{"id":"T8","span":{"begin":1101,"end":1112},"obj":"Microorganism"}],"text":"Isolation, expression and characterization of a minor allergen from Penicillium crustosum.\nA ribosomal P1 protein, Pen b 26 from Penicillium brevicompactum, was previously identified as a major allergen. A homolog protein was isolated and characterized from Penicillium crustosum which is not known to be allergenic mold. A cDNA library of P. crustosum was constructed and screened using a probe based on the DNA sequence of Pen b 26. A positive clone was isolated, expressed in Escherichia coli, purified and characterized by comparing its immunological and physical properties to Pen b 26. It was designated as Pen cr 26 and had a 321 nt ORF corresponding to 107 amino acids with a MW of 11 kDa. Pen cr 26 had strong sequence homology to Pen b 26 (92% for nucleotides and 86% for amino acids) and its physical and predicted structural properties were similar to the latter. The level of expression of Pen cr 26 was much lower than that of Pen b 26 in the same expression vector. Both proteins were recognized equally well by the IgG class specific antibodies, but Pen cr 26 was poorly recognized by Penicillium-sensitive atopic sera (IgE), suggesting striking antigenic difference in IgE epitopes, i.e., 87% were positive for Pen b 26 while only 23% were positive for Pen cr 26. The allergenicity of Pen cr 26 seems to be minor in nature and it could be a hypoallergenic variant of Pen b 26. \n\n"}