BB-rel@ldeleger:BB-rel-19049879 / 89-1466
Annnotations
bionlp-ost-19-BB-rel-train
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Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T2 | 0-1377 | Paragraph | denotes | Myeloid differentiation factor 88 (MyD88) is an adaptor protein involved in the interleukin-1 receptor and Toll-like receptor-induced activation of nuclear factor-kappaB (NF-kappaB). In this report, the full-length cDNA of MyD88 was cloned from the large yellow croaker, Pseudosciaena crocea. It was of 1574 bp, including a 5'-terminal untranslated region (UTR) of 89 bp, a 3'-terminal UTR of 621bp and an open reading frame (ORF) of 864 bp encoding a polypeptide of 287 amino acids. It contained a typical death domain at the N-terminal and a conservative Toll/IL-1R (TIR) domain structure at the C-terminal. The quantitative real-time reverse transcription PCR analysis revealed a broad expression of MyD88 with the highest expression in the spleen and the weakest expression in the muscle. The expression of MyD88 after challenge with formalin-inactivated Gram-negative bacterium Vibrio parahaemolyticus was tested in blood, spleen and liver. It suggested that the highest expression was in the spleen (p<0.05) with 1.9 times (at 48 h) as much as that in the control and the lowest expression of MyD88 was in the liver (p<0.05) with 0.29 times (at 3h) of that in the control. These results indicated that as a universal key adaptor in the Toll-like receptor pathway in mammals, MyD88 might play an important role in large yellow croaker defense against pathogenic infection. |
T5 | 249-269 | Habitat | denotes | large yellow croaker |
T6 | 271-291 | Habitat | denotes | Pseudosciaena crocea |
T7 | 744-750 | Habitat | denotes | spleen |
T8 | 785-791 | Habitat | denotes | muscle |
T9 | 859-872 | Phenotype | denotes | Gram-negative |
T10 | 883-906 | Microorganism | denotes | Vibrio parahaemolyticus |
T11 | 921-926 | Habitat | denotes | blood |
T12 | 928-934 | Habitat | denotes | spleen |
T13 | 939-944 | Habitat | denotes | liver |
T14 | 998-1004 | Habitat | denotes | spleen |
T15 | 1116-1121 | Habitat | denotes | liver |
T16 | 1272-1279 | Habitat | denotes | mammals |
T17 | 1319-1339 | Habitat | denotes | large yellow croaker |
T18 | 1356-1366 | Phenotype | denotes | pathogenic |
E1 | 883-906 | Exhibits | denotes | Vibrio parahaemolyticus |
R1 | E1 | T10 | Microorganism | Vibrio parahaemolyticus,Vibrio parahaemolyticus |
R2 | E1 | T9 | Property | Vibrio parahaemolyticus,Gram-negative |