BB-rel@ldeleger:BB-rel-19049879 JSONTXT

{"project":"bionlp-ost-19-BB-rel-train","denotations":[{"id":"T1","span":{"begin":0,"end":88},"obj":"Title"},{"id":"T3","span":{"begin":45,"end":65},"obj":"Habitat"},{"id":"T4","span":{"begin":67,"end":87},"obj":"Habitat"},{"id":"T2","span":{"begin":89,"end":1466},"obj":"Paragraph"},{"id":"T5","span":{"begin":338,"end":358},"obj":"Habitat"},{"id":"T6","span":{"begin":360,"end":380},"obj":"Habitat"},{"id":"T7","span":{"begin":833,"end":839},"obj":"Habitat"},{"id":"T8","span":{"begin":874,"end":880},"obj":"Habitat"},{"id":"T9","span":{"begin":948,"end":961},"obj":"Phenotype"},{"id":"T10","span":{"begin":972,"end":995},"obj":"Microorganism"},{"id":"T11","span":{"begin":1010,"end":1015},"obj":"Habitat"},{"id":"T12","span":{"begin":1017,"end":1023},"obj":"Habitat"},{"id":"T13","span":{"begin":1028,"end":1033},"obj":"Habitat"},{"id":"T14","span":{"begin":1087,"end":1093},"obj":"Habitat"},{"id":"T15","span":{"begin":1205,"end":1210},"obj":"Habitat"},{"id":"T16","span":{"begin":1361,"end":1368},"obj":"Habitat"},{"id":"T17","span":{"begin":1408,"end":1428},"obj":"Habitat"},{"id":"T18","span":{"begin":1445,"end":1455},"obj":"Phenotype"},{"id":"E1","span":{"begin":972,"end":995},"obj":"Exhibits"}],"relations":[{"id":"R1","pred":"Microorganism","subj":"E1","obj":"T10"},{"id":"R2","pred":"Property","subj":"E1","obj":"T9"}],"text":"Molecular cloning and expression of MyD88 in large yellow croaker, Pseudosciaena crocea.\nMyeloid differentiation factor 88 (MyD88) is an adaptor protein involved in the interleukin-1 receptor and Toll-like receptor-induced activation of nuclear factor-kappaB (NF-kappaB). In this report, the full-length cDNA of MyD88 was cloned from the large yellow croaker, Pseudosciaena crocea. It was of 1574 bp, including a 5'-terminal untranslated region (UTR) of 89 bp, a 3'-terminal UTR of 621bp and an open reading frame (ORF) of 864 bp encoding a polypeptide of 287 amino acids. It contained a typical death domain at the N-terminal and a conservative Toll/IL-1R (TIR) domain structure at the C-terminal. The quantitative real-time reverse transcription PCR analysis revealed a broad expression of MyD88 with the highest expression in the spleen and the weakest expression in the muscle. The expression of MyD88 after challenge with formalin-inactivated Gram-negative bacterium Vibrio parahaemolyticus was tested in blood, spleen and liver. It suggested that the highest expression was in the spleen (p\u003c0.05) with 1.9 times (at 48 h) as much as that in the control and the lowest expression of MyD88 was in the liver (p\u003c0.05) with 0.29 times (at 3h) of that in the control. These results indicated that as a universal key adaptor in the Toll-like receptor pathway in mammals, MyD88 might play an important role in large yellow croaker defense against pathogenic infection.\n\n"}