BB-rel@ldeleger:BB-rel-10639454 / 161-2493 JSONTXT

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bionlp-ost-19-BB-rel-test

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Id Subject Object Predicate Lexical cue
T2 0-2332 Paragraph denotes Some Escherichia coli strains isolated from intestinal or extraintestinal infections in pigs produce cytotoxic necrotizing factor 1 (CNF1). In order to analyze the role of CNF1 in the pathogenesis of porcine colibacillosis, newborn colostrum-deprived germfree piglets were orally inoculated with a wild-type CNF1-producing strain (M623) or with an isogenic cnf1 mutant (M623DeltaCNF1). The two isogenic strains induced a high mortality with similar lung and serosal inflammatory lesions, indicating that both strains were pathogenic in these piglets. Bacterial counts in various organs of inoculated piglets revealed an intestinal predisposition of M623 and M623DeltaCNF1 strains for the cecum and colon. Extraintestinal organs (lungs, liver, spleen, and kidney) were also colonized by both strains. Similar colonization of intestinal and extraintestinal tissues in animals inoculated with either strain was observed, except in the ileum, where M623 showed a higher colonization than M623DeltaCNF1. Intestinal (ileum and colon), extraintestinal (lung and kidney), and immune (mesenteric lymph nodes and spleen) tissues were sampled at 1 day postinoculation and analyzed for cytokine expression by a reverse transcriptase PCR technique. Inoculation with E. coli M623 induced an enhanced expression of inflammatory cytokines (interleukin-1alpha [IL-1alpha], tumor necrosis factor alpha, and IL-12p40) in the intestinal organs compared to uninoculated piglets or piglets inoculated with nonpathogenic intestinal E. coli 862B, which is also able to colonize the intestinal tract. There was little difference in cytokine transcript levels in the intestinal and extraintestinal organs in piglets inoculated with E. coli strains M623 or M623DeltaCNF1, except in the ileum, where IL-1alpha and IL-8 mRNA levels correlated with bacterial colonization. Expression of regulatory cytokines (gamma interferon and IL-4) was weak in immune tissues from piglets inoculated with M623 or M623DeltaCNF1. Taken together, our data indicate that the CNF1-producing strain, M623, is pathogenic and induces inflammatory cytokine expression in germfree, colostrum-deprived piglets. Nevertheless, in this model, the CNF1 toxin does not appear to be a major factor for pathogenicity or cytokine response, as demonstrated by the use of an isogenic cnf1 mutant.
T6 5-21 Microorganism denotes Escherichia coli
T7 44-54 Habitat denotes intestinal
T8 58-73 Habitat denotes extraintestinal
T9 88-92 Habitat denotes pigs
T10 200-207 Habitat denotes porcine
T11 224-267 Habitat denotes newborn colostrum-deprived germfree piglets
T12 232-241 Habitat denotes colostrum
T13 298-307 Phenotype denotes wild-type
T14 308-322 Phenotype denotes CNF1-producing
T15 331-335 Microorganism denotes M623
T16 348-368 Phenotype denotes isogenic cnf1 mutant
T17 370-383 Microorganism denotes M623DeltaCNF1
T18 449-453 Habitat denotes lung
T19-1 449-453,466-486 Habitat denotes lung inflammatory lesions
T20 458-486 Habitat denotes serosal inflammatory lesions
T21 458-465 Habitat denotes serosal
T22 522-532 Phenotype denotes pathogenic
T23 542-549 Habitat denotes piglets
T24 579-607 Habitat denotes organs of inoculated piglets
T25 589-607 Habitat denotes inoculated piglets
T26 620-630 Habitat denotes intestinal
T27 649-653 Microorganism denotes M623
T28 658-671 Microorganism denotes M623DeltaCNF1
T29 688-693 Habitat denotes cecum
T30 698-703 Habitat denotes colon
T32 705-727 Habitat denotes Extraintestinal organs
T31 705-720 Habitat denotes Extraintestinal
T33 729-734 Habitat denotes lungs
T34 736-741 Habitat denotes liver
T35 743-749 Habitat denotes spleen
T36 755-761 Habitat denotes kidney
T38 824-834 Habitat denotes intestinal
T37-1 824-834,855-862 Habitat denotes intestinal tissues
T39 839-862 Habitat denotes extraintestinal tissues
T40 839-854 Habitat denotes extraintestinal
T41 866-903 Habitat denotes animals inoculated with either strain
T42 932-937 Habitat denotes ileum
T43 945-949 Microorganism denotes M623
T44 984-997 Microorganism denotes M623DeltaCNF1
T46 999-1009 Habitat denotes Intestinal
T45-1 999-1009,1111-1118 Habitat denotes Intestinal tissues
T47 1011-1016 Habitat denotes ileum
T48 1021-1026 Habitat denotes colon
T50 1029-1044 Habitat denotes extraintestinal
T49-1 1029-1044,1111-1118 Habitat denotes extraintestinal tissues
T51 1046-1050 Habitat denotes lung
T52 1055-1061 Habitat denotes kidney
T53-1 1068-1074,1111-1118 Habitat denotes immune tissues
T54 1076-1098 Habitat denotes mesenteric lymph nodes
T55 1103-1109 Habitat denotes spleen
T56 1253-1265 Microorganism denotes E. coli M623
T57 1406-1423 Habitat denotes intestinal organs
T58 1406-1416 Habitat denotes intestinal
T59 1436-1456 Habitat denotes uninoculated piglets
T60 1460-1521 Habitat denotes piglets inoculated with nonpathogenic intestinal E. coli 862B
T61 1484-1497 Phenotype denotes nonpathogenic
T62 1498-1508 Habitat denotes intestinal
T63 1509-1521 Microorganism denotes E. coli 862B
T64 1558-1574 Habitat denotes intestinal tract
T65-1 1641-1651,1672-1678 Habitat denotes intestinal organs
T66 1656-1678 Habitat denotes extraintestinal organs
T68 1682-1726 Habitat denotes piglets inoculated with E. coli strains M623
T67-1 1682-1705,1730-1743 Habitat denotes piglets inoculated with M623DeltaCNF1
T69 1706-1726 Microorganism denotes E. coli strains M623
T70-1 1706-1721,1730-1743 Microorganism denotes E. coli strains M623DeltaCNF1
T71 1759-1764 Habitat denotes ileum
T72 1918-1966 Habitat denotes immune tissues from piglets inoculated with M623
T73-1 1918-1961,1970-1983 Habitat denotes immune tissues from piglets inoculated with M623DeltaCNF1
T74 1938-1966 Habitat denotes piglets inoculated with M623
T75-1 1938-1961,1970-1983 Habitat denotes piglets inoculated with M623DeltaCNF1
T76 1962-1966 Microorganism denotes M623
T77 1970-1983 Microorganism denotes M623DeltaCNF1
T78 2028-2042 Phenotype denotes CNF1-producing
T79 2051-2055 Microorganism denotes M623
T80 2060-2070 Phenotype denotes pathogenic
T81 2119-2155 Habitat denotes germfree, colostrum-deprived piglets
T82 2129-2138 Habitat denotes colostrum
T83 2242-2255 Phenotype denotes pathogenicity
T84 2311-2331 Phenotype denotes isogenic cnf1 mutant