BB-norm@ldeleger:BB-norm-351567 / 73-1055 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	NCBI_Taxonomy	OntoBiotope
T2	0-982	Paragraph	denotes	Cell-free extracts from M-13 am5 infected Escherichia coli cells which are highly concentrated on cellophane membrane disks replicate efficiently endogenous M-13 duplex DNA. If the reaction is carried out in the presence of bromodeoxyuridine triphosphate, the majority of the label is found in two classes of hybrid DNA molecules in which either the viral or the complementary strand is newly synthesized. A minor portion of the label is incorporated into fully synthetic duplex DNA. DNA synthesis requires ATP and is inhibited by nalidixic acid, novobiocin, and arabinosylnucleoside triphosphates. Rifampicin blocks preferentially the synthesis of molecules with labeled complementary strands. A similar effect is observed upon addition of the helix-destabilising M-13 gene V protein. In contrast, addition of E. coli helix-destabilising protein (Eco HD-protein) stimulates the synthesis of both types of hybrid DNA molecules as well as the formation of fully synthetic duplex DNA.
T4	24-32	Microorganism	denotes	M-13 am5	1977402
T5	29-32	Phenotype	denotes	am5		OBT:000118
T6	42-58	Microorganism	denotes	Escherichia coli	562
T7	98-123	Habitat	denotes	cellophane membrane disks		OBT:000103
T8	157-161	Microorganism	denotes	M-13	1977402
T9	765-769	Microorganism	denotes	M-13	1977402
T10	811-818	Microorganism	denotes	E. coli	562