BB-norm@ldeleger:BB-norm-25114119 JSONTXT

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bionlp-ost-19-BB-norm-dev

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Id Subject Object Predicate Lexical cue NCBI_Taxonomy OntoBiotope
T1 0-199 Title denotes Characterization of a lipopolysaccharide-targeted monoclonal antibody and its variable fragments as candidates for prophylaxis against the obligate intracellular bacterial pathogen Coxiella burnetii.
T3 139-161 Phenotype denotes obligate intracellular OBT:000771
T4 172-180 Phenotype denotes pathogen OBT:000375
T5 181-198 Microorganism denotes Coxiella burnetii 777
T2 200-1966 Paragraph denotes Our previous study demonstrated that treatment of Coxiella burnetii with the phase I lipopolysaccharide (PI-LPS)-targeted monoclonal antibody (MAb) 1E4 significantly inhibited C. burnetii infection in mice, suggesting that 1E4 is a protective MAb. To determine whether passive transfer of antibodies (Abs) can provide protection against C. burnetii natural infection, we examined if passive transfer of 1E4 would protect SCID mice against C. burnetii aerosol infection. The results indicated that 1E4 conferred significant protection against aerosolized C. burnetii, suggesting that 1E4 may be useful for preventing C. burnetii natural infection. To further understand the mechanisms of 1E4-mediated protection and to test the possibility of using humanized 1E4 to prevent C. burnetii infection, we examined whether the Fab fragment of 1E4 (Fab1E4), a recombinant murine single-chain variable fragment (muscFv1E4), and a humanized single-chain variable fragment (huscFv1E4) retained the ability of 1E4 to inhibit C. burnetii infection. The results indicated that Fab1E4, muscFv1E4, and huscFv1E4 were able to inhibit C. burnetii infection in mice but that their ability to inhibit C. burnetii infection was lower than that of 1E4. In addition, treatment of C. burnetii with Fab1E4, muscFv1E4, or huscFv1E4 can block C. burnetii infection of macrophages. Interestingly, treatment of C. burnetii with huscFv1E4 can significantly reduce C. burnetii infectivity in human macrophages. This report provides the first evidence to demonstrate that the humanized variable fragments of an LPS-specific MAb can neutralize C. burnetii infection and appears to be a promising step toward the potential use of a humanized MAb as emergency prophylaxis against C. burnetii exposure.
T6 250-267 Microorganism denotes Coxiella burnetii 777
T7 376-387 Microorganism denotes C. burnetii 777
T8 401-405 Habitat denotes mice OBT:003211
T9 537-548 Microorganism denotes C. burnetii 777
T10 621-630 Habitat denotes SCID mice OBT:003211
T11 639-650 Microorganism denotes C. burnetii 777
T12 651-658 Habitat denotes aerosol OBT:000030
T13 742-753 Habitat denotes aerosolized OBT:000030
T14 754-765 Microorganism denotes C. burnetii 777
T15 816-827 Microorganism denotes C. burnetii 777
T16 973-984 Microorganism denotes C. burnetii 777
T17 1064-1070 Habitat denotes murine OBT:002530
T18 1213-1224 Microorganism denotes C. burnetii 777
T19 1317-1328 Microorganism denotes C. burnetii 777
T20 1342-1346 Habitat denotes mice OBT:003211
T21 1381-1392 Microorganism denotes C. burnetii 777
T22 1457-1468 Microorganism denotes C. burnetii 777
T23 1516-1527 Microorganism denotes C. burnetii 777
T24 1541-1552 Habitat denotes macrophages OBT:002995
T25 1582-1593 Microorganism denotes C. burnetii 777
T26 1634-1645 Microorganism denotes C. burnetii 777
T28 1661-1678 Habitat denotes human macrophages OBT:002995
T27 1661-1666 Habitat denotes human OBT:002488
T29 1811-1822 Microorganism denotes C. burnetii 777
T30 1945-1956 Microorganism denotes C. burnetii 777