BB-norm@ldeleger:BB-norm-16629992
Annnotations
bionlp-ost-19-BB-norm-test
Below, discontinuous spans are shown in the chain model. You can change it to the bag model.
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-148 | Title | denotes | Sensitive and rapid detection of Flavobacterium columnare in channel catfish Ictalurus punctatus by a loop-mediated isothermal amplification method. |
| T6 | 33-57 | Microorganism | denotes | Flavobacterium columnare |
| T8 | 61-76 | Habitat | denotes | channel catfish |
| T7 | 61-68 | Habitat | denotes | channel |
| T9 | 77-96 | Habitat | denotes | Ictalurus punctatus |
| T2 | 149-388 | Paragraph | denotes | To evaluate the loop-mediated isothermal amplification method (LAMP) for rapid detection of Flavobacterium columnare and determine the suitability of LAMP for rapid diagnosis of columnaris infection in channel catfish, Ictalurus punctatus. |
| T10 | 241-265 | Microorganism | denotes | Flavobacterium columnare |
| T11 | 351-366 | Habitat | denotes | channel catfish |
| T12 | 351-358 | Habitat | denotes | channel |
| T13 | 368-387 | Habitat | denotes | Ictalurus punctatus |
| T3 | 389-1239 | Paragraph | denotes | A set of four primers, two outer and two inner, were designed specifically to recognize 16S ribosomal RNA gene of this pathogen. Bacterial genomic DNA templates were prepared by hot lysis in a lysis buffer. Amplification of the specific gene segments was carried out at 65 degrees C for 1 h. The amplified gene products were analysed by agarose gel electrophoresis and detected by staining gels with ethidium bromide. A PCR assay was also included in this study. Our results demonstrate that the ladder-like pattern of bands from 204 bp specific to the Fl. columnare 16S ribosomal RNA gene was amplified. The detection limit of the LAMP assay was comparable to that of PCR in prepared genomic DNA reactions. In addition, this optimized LAMP assay was able to detect the Fl. columnare 16S ribosomal RNA gene in experimentally infected channel catfish. |
| T14 | 508-516 | Phenotype | denotes | pathogen |
| T15 | 942-955 | Microorganism | denotes | Fl. columnare |
| T16 | 1159-1172 | Microorganism | denotes | Fl. columnare |
| T17 | 1199-1238 | Habitat | denotes | experimentally infected channel catfish |
| T18 | 1223-1230 | Habitat | denotes | channel |
| T4 | 1240-1318 | Paragraph | denotes | The LAMP assay for Fl. columnare detection in channel catfish was established. |
| T19 | 1259-1272 | Microorganism | denotes | Fl. columnare |
| T20 | 1286-1301 | Habitat | denotes | channel catfish |
| T21 | 1286-1293 | Habitat | denotes | channel |
| T5 | 1319-1534 | Paragraph | denotes | Because LAMP assay is a rapid, sensitive, specific, simple and cost-effective assay for Fl. columnare detection in channel catfish, it is useful for rapid diagnosis of Fl. columnare in fish hatcheries and the field. |
| T22 | 1407-1420 | Microorganism | denotes | Fl. columnare |
| T24 | 1434-1449 | Habitat | denotes | channel catfish |
| T23 | 1434-1441 | Habitat | denotes | channel |
| T25 | 1487-1500 | Microorganism | denotes | Fl. columnare |
| T26 | 1504-1519 | Habitat | denotes | fish hatcheries |
| T27 | 1504-1508 | Habitat | denotes | fish |