BB-norm+ner@ldeleger:BB-norm+ner-19494280 / 127-1238 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/BB-norm+ner@ldeleger/sourceid/BB-norm+ner-19494280","sourcedb":"BB-norm+ner@ldeleger","sourceid":"BB-norm+ner-19494280","text":"Regulation of cytotoxic effector molecule expression in human CTLs after viral or bacterial activation is poorly understood. By using human autologous dendritic cells (DCs) to prime T lymphocytes, we found perforin only highly up-regulated in virus- (HSV-1, vaccinia virus) but not in intracellular bacteria- (Listeria innocua, Listeria monocytogenes, Mycobacterium tuberculosis, Chlamydophila pneumoniae) activated CTLs. In contrast, larger quantities of IFN-gamma and TNF-alpha were produced in Listeria-stimulated cultures. Granzyme B and granulysin were similarly up-regulated by all tested viruses and intracellular bacteria. DCs infected with HSV-1 showed enhanced surface expression of the costimulatory molecule CD252 (CD134L) compared with Listeria-infected DC and induced enhanced secretion of IL-2. Adding blocking CD134 or neutralizing IL-2 Abs during T cell activation reduced the HSV-dependent up-regulation of perforin. These data indicate a distinct CTL effector function in response to intracellular pathogens triggered via differing endogenous IL-2 production upon costimulation through CD252.","tracks":[{"project":"bionlp-ost-19-BB-norm-ner-test","denotations":[{"id":"T2","span":{"begin":0,"end":1111},"obj":"Paragraph"}],"attributes":[{"subj":"T2","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"bionlp-ost-19-BB-norm-ner-test","color":"#b093ec","default":true}]}]}}