BB-norm+ner@ldeleger:BB-norm+ner-18789156 / 690-1808 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/BB-norm+ner@ldeleger/sourceid/BB-norm+ner-18789156","sourcedb":"BB-norm+ner@ldeleger","sourceid":"BB-norm+ner-18789156","text":"We show here that cell-surface expressed nucleolin is a receptor for Francisella tularensis Live Vaccine Strain (LVS) and promotes LVS binding and infection of human monocyte-like THP-1 cells. The HB-19 pseudopeptide that binds specifically carboxy-terminal RGG domain of nucleolin inhibits LVS binding and infection of monocyte-like THP-1 cells. In a pull-down assay, elongation factor Tu (EF-Tu), a GTP-binding protein involved in protein translation, usually found in cytoplasm, was recovered among LVS bacterial membrane proteins bound on RGG domain of nucleolin. A specific polyclonal murine antibody was raised against recombinant LVS EF-Tu. By fluorescence and electron microscopy experiments, we found that a fraction of EF-Tu could be detected at the bacterial surface. Anti-EF-Tu antibodies reduced LVS binding to monocyte-like THP-1 cells and impaired infection, even in absence of complement and complement receptors. Interaction between EF-Tu and nucleolin was illustrated by two different pull-down assays using recombinant EF-Tu proteins and either RGG domain of nucleolin or cell solubilized nucleolin.","tracks":[{"project":"bionlp-ost-19-BB-norm-ner-dev","denotations":[{"id":"T3","span":{"begin":0,"end":1118},"obj":"Paragraph"},{"id":"T15","span":{"begin":18,"end":22},"obj":"Habitat"},{"id":"T16","span":{"begin":69,"end":111},"obj":"Microorganism"},{"id":"T17","span":{"begin":113,"end":116},"obj":"Microorganism"},{"id":"T18","span":{"begin":131,"end":134},"obj":"Microorganism"},{"id":"T19","span":{"begin":135,"end":142},"obj":"Phenotype"},{"id":"T21","span":{"begin":160,"end":191},"obj":"Habitat"},{"id":"T20","span":{"begin":160,"end":165},"obj":"Habitat"},{"id":"T22","span":{"begin":166,"end":191},"obj":"Habitat"},{"id":"T23","span":{"begin":291,"end":294},"obj":"Microorganism"},{"id":"T24","span":{"begin":295,"end":302},"obj":"Phenotype"},{"id":"T25","span":{"begin":320,"end":345},"obj":"Habitat"},{"id":"T26","span":{"begin":502,"end":505},"obj":"Microorganism"},{"id":"T27","span":{"begin":590,"end":596},"obj":"Habitat"},{"id":"T28","span":{"begin":637,"end":640},"obj":"Microorganism"},{"id":"T29","span":{"begin":809,"end":812},"obj":"Microorganism"},{"id":"T30","span":{"begin":813,"end":820},"obj":"Phenotype"},{"id":"T31","span":{"begin":824,"end":849},"obj":"Habitat"},{"id":"T32","span":{"begin":1091,"end":1095},"obj":"Habitat"}],"attributes":[{"id":"A14","pred":"NCBI_Taxonomy","subj":"T17","obj":"263"},{"id":"A28","pred":"NCBI_Taxonomy","subj":"T28","obj":"263"},{"id":"A27","pred":"OntoBiotope","subj":"T27","obj":"OBT:002530"},{"id":"A20","pred":"OntoBiotope","subj":"T22","obj":"OBT:003001"},{"id":"A21","pred":"OntoBiotope","subj":"T22","obj":"OBT:000062"},{"id":"A15","pred":"NCBI_Taxonomy","subj":"T18","obj":"263"},{"id":"A33","pred":"OntoBiotope","subj":"T32","obj":"OBT:000061"},{"id":"A13","pred":"NCBI_Taxonomy","subj":"T16","obj":"263"},{"id":"A30","pred":"OntoBiotope","subj":"T30","obj":"OBT:000029"},{"id":"A22","pred":"NCBI_Taxonomy","subj":"T23","obj":"263"},{"id":"A12","pred":"OntoBiotope","subj":"T15","obj":"OBT:000061"},{"id":"A17","pred":"OntoBiotope","subj":"T20","obj":"OBT:002488"},{"id":"A26","pred":"NCBI_Taxonomy","subj":"T26","obj":"263"},{"id":"A31","pred":"OntoBiotope","subj":"T31","obj":"OBT:003001"},{"id":"A32","pred":"OntoBiotope","subj":"T31","obj":"OBT:000062"},{"id":"A29","pred":"NCBI_Taxonomy","subj":"T29","obj":"263"},{"id":"A18","pred":"OntoBiotope","subj":"T21","obj":"OBT:000062"},{"id":"A19","pred":"OntoBiotope","subj":"T21","obj":"OBT:003001"},{"id":"A16","pred":"OntoBiotope","subj":"T19","obj":"OBT:000029"},{"id":"A23","pred":"OntoBiotope","subj":"T24","obj":"OBT:000029"},{"id":"A24","pred":"OntoBiotope","subj":"T25","obj":"OBT:000062"},{"id":"A25","pred":"OntoBiotope","subj":"T25","obj":"OBT:003001"},{"subj":"T3","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T15","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T16","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T17","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T18","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T19","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T21","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T20","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T22","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T23","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T24","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T25","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T26","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T27","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T28","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T29","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T30","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T31","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"},{"subj":"T32","pred":"source","obj":"bionlp-ost-19-BB-norm-ner-dev"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"bionlp-ost-19-BB-norm-ner-dev","color":"#a9ec93","default":true}]}]}}