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bionlp-ost-19-BB-kb-dev

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Id Subject Object Predicate Lexical cue NCBI_Taxonomy OntoBiotope
T1 0-173 Title denotes A novel receptor - ligand pathway for entry of Francisella tularensis in monocyte-like THP-1 cells: interaction between surface nucleolin and bacterial elongation factor Tu.
T5 47-69 Microorganism denotes Francisella tularensis 263
T6 73-98 Habitat denotes monocyte-like THP-1 cells OBT:000062|OBT:003001
T2 174-689 Paragraph denotes Francisella tularensis, the causative agent of tularemia, is one of the most infectious human bacterial pathogens. It is phagocytosed by immune cells, such as monocytes and macrophages. The precise mechanisms that initiate bacterial uptake have not yet been elucidated. Participation of C3, CR3, class A scavenger receptors and mannose receptor in bacterial uptake have been already reported. However, contribution of an additional, as-yet-unidentified receptor for F. tularensis internalization has been suggested.
T7 174-196 Microorganism denotes Francisella tularensis 263
T8 202-230 Phenotype denotes causative agent of tularemia OBT:002806
T10 262-287 Phenotype denotes human bacterial pathogens OBT:002806
T9 262-267 Habitat denotes human OBT:002488
T11 311-323 Habitat denotes immune cells OBT:000320
T12 333-342 Habitat denotes monocytes OBT:003001
T13 347-358 Habitat denotes macrophages OBT:002995
T14 640-653 Microorganism denotes F. tularensis 263
T3 690-1808 Paragraph denotes We show here that cell-surface expressed nucleolin is a receptor for Francisella tularensis Live Vaccine Strain (LVS) and promotes LVS binding and infection of human monocyte-like THP-1 cells. The HB-19 pseudopeptide that binds specifically carboxy-terminal RGG domain of nucleolin inhibits LVS binding and infection of monocyte-like THP-1 cells. In a pull-down assay, elongation factor Tu (EF-Tu), a GTP-binding protein involved in protein translation, usually found in cytoplasm, was recovered among LVS bacterial membrane proteins bound on RGG domain of nucleolin. A specific polyclonal murine antibody was raised against recombinant LVS EF-Tu. By fluorescence and electron microscopy experiments, we found that a fraction of EF-Tu could be detected at the bacterial surface. Anti-EF-Tu antibodies reduced LVS binding to monocyte-like THP-1 cells and impaired infection, even in absence of complement and complement receptors. Interaction between EF-Tu and nucleolin was illustrated by two different pull-down assays using recombinant EF-Tu proteins and either RGG domain of nucleolin or cell solubilized nucleolin.
T15 708-712 Habitat denotes cell OBT:000061
T16 759-801 Microorganism denotes Francisella tularensis Live Vaccine Strain 263
T17 803-806 Microorganism denotes LVS 263
T18 821-824 Microorganism denotes LVS 263
T19 825-832 Phenotype denotes binding OBT:000029
T21 850-881 Habitat denotes human monocyte-like THP-1 cells OBT:000062|OBT:003001
T20 850-855 Habitat denotes human OBT:002488
T22 856-881 Habitat denotes monocyte-like THP-1 cells OBT:003001|OBT:000062
T23 981-984 Microorganism denotes LVS 263
T24 985-992 Phenotype denotes binding OBT:000029
T25 1010-1035 Habitat denotes monocyte-like THP-1 cells OBT:000062|OBT:003001
T26 1192-1195 Microorganism denotes LVS 263
T27 1280-1286 Habitat denotes murine OBT:002530
T28 1327-1330 Microorganism denotes LVS 263
T29 1499-1502 Microorganism denotes LVS 263
T30 1503-1510 Phenotype denotes binding OBT:000029
T31 1514-1539 Habitat denotes monocyte-like THP-1 cells OBT:003001|OBT:000062
T32 1781-1785 Habitat denotes cell OBT:000061
T4 1809-2536 Paragraph denotes Altogether, our results demonstrate that the interaction between surface nucleolin and its bacterial ligand EF-Tu plays an important role in Francisella tularensis adhesion and entry process and may therefore facilitate invasion of host tissues. Since phagosomal escape and intra-cytosolic multiplication of LVS in infected monocytes are very similar to those of human pathogenic F. tularensis ssp tularensis, the mechanism of entry into monocyte-like THP-1 cells, involving interaction between EF-Tu and nucleolin, might be similar in the two subspecies. Thus, the use of either nucleolin-specific pseudopeptide HB-19 or recombinant EF-Tu could provide attractive therapeutic approaches for modulating F. tularensis infection.
T33 1950-1972 Microorganism denotes Francisella tularensis 263
T34 1973-1981 Phenotype denotes adhesion OBT:000029
T35 2029-2053 Phenotype denotes invasion of host tissues OBT:002806
T36 2041-2053 Habitat denotes host tissues OBT:000196
T37 2117-2120 Microorganism denotes LVS 263
T38 2124-2142 Habitat denotes infected monocytes OBT:003001
T40 2172-2188 Phenotype denotes human pathogenic OBT:002806
T39 2172-2177 Habitat denotes human OBT:002488
T41 2189-2217 Microorganism denotes F. tularensis ssp tularensis 119856
T42 2247-2272 Habitat denotes monocyte-like THP-1 cells OBT:003001|OBT:000062
T43 2512-2525 Microorganism denotes F. tularensis 263
E1 47-69 Lives_In denotes Francisella tularensis
E4 174-196 Lives_In denotes Francisella tularensis
E2 174-196 Exhibits denotes Francisella tularensis
E3 174-196 Exhibits denotes Francisella tularensis
E6 821-824 Lives_In denotes LVS
E7 821-824 Lives_In denotes LVS
E5 821-824 Exhibits denotes LVS
E9 981-984 Lives_In denotes LVS
E8 981-984 Exhibits denotes LVS
E10 1499-1502 Exhibits denotes LVS
E13 1950-1972 Lives_In denotes Francisella tularensis
E11 1950-1972 Exhibits denotes Francisella tularensis
E12 1950-1972 Exhibits denotes Francisella tularensis
E14 2117-2120 Lives_In denotes LVS
E15 2117-2120 Lives_In denotes LVS
E17 2189-2217 Lives_In denotes F. tularensis ssp tularensis
E18 2189-2217 Lives_In denotes F. tularensis ssp tularensis
E16 2189-2217 Exhibits denotes F. tularensis ssp tularensis
R1 E1 T5 Microorganism Francisella tularensis,Francisella tularensis
R2 E1 T6 Location Francisella tularensis,monocyte-like THP-1 cells
R3 E4 T7 Microorganism Francisella tularensis,Francisella tularensis
R4 E4 T9 Location Francisella tularensis,human
R5 E2 T7 Microorganism Francisella tularensis,Francisella tularensis
R6 E2 T10 Property Francisella tularensis,human bacterial pathogens
R7 E3 T7 Microorganism Francisella tularensis,Francisella tularensis
R8 E3 T8 Property Francisella tularensis,causative agent of tularemia
R9 E6 T18 Microorganism LVS,LVS
R10 E6 T21 Location LVS,human monocyte-like THP-1 cells
R11 E7 T18 Microorganism LVS,LVS
R12 E7 T20 Location LVS,human
R13 E5 T18 Microorganism LVS,LVS
R14 E5 T19 Property LVS,binding
R15 E9 T23 Microorganism LVS,LVS
R16 E9 T25 Location LVS,monocyte-like THP-1 cells
R17 E8 T23 Microorganism LVS,LVS
R18 E8 T24 Property LVS,binding
R19 E10 T29 Microorganism LVS,LVS
R20 E10 T30 Property LVS,binding
R21 E13 T33 Microorganism Francisella tularensis,Francisella tularensis
R22 E13 T36 Location Francisella tularensis,host tissues
R23 E11 T33 Microorganism Francisella tularensis,Francisella tularensis
R24 E11 T34 Property Francisella tularensis,adhesion
R25 E12 T33 Microorganism Francisella tularensis,Francisella tularensis
R26 E12 T35 Property Francisella tularensis,invasion of host tissues
R27 E14 T37 Microorganism LVS,LVS
R28 E14 T38 Location LVS,infected monocytes
R29 E15 T37 Microorganism LVS,LVS
R30 E15 T42 Location LVS,monocyte-like THP-1 cells
R31 E17 T41 Microorganism F. tularensis ssp tularensis,F. tularensis ssp tularensis
R32 E17 T39 Location F. tularensis ssp tularensis,human
R33 E18 T41 Microorganism F. tularensis ssp tularensis,F. tularensis ssp tularensis
R34 E18 T42 Location F. tularensis ssp tularensis,monocyte-like THP-1 cells
R35 E16 T41 Microorganism F. tularensis ssp tularensis,F. tularensis ssp tularensis
R36 E16 T40 Property F. tularensis ssp tularensis,human pathogenic