BB-kb+ner@ldeleger:BB-kb+ner-8559802 JSONTXT

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bionlp-ost-19-BB-kb-ner-dev

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Id Subject Object Predicate Lexical cue NCBI_Taxonomy OntoBiotope
T1 0-94 Title denotes Construction of a cloning vector from a naturally occurring plasmid of Salmonella typhimurium.
T2 95-986 Paragraph denotes A naturally occurring plasmid isolated from a drug-resistant strain of Salmonella typhimurium (993) has been used to construct a plasmid vector for cloning in a wild strain of Salmonella. The strain (993) contains at least two plasmids. The smaller plasmid (9 kb) contains an ampicillin-resistant marker, while the larger one (25 kb) is cryptic. Physical mapping of the 9-kb plasmid and construction of a 3.5-kb derivative have been carried out. This plasmid has been used for cloning in a restriction+modification+strain of S. typhimurium using a conventional calcium chloride method. It exhibited better efficiency of transformation than other commonly used plasmids such as pBR322 or its derivatives and transformants were found to be stable in the absence of antibiotic selection. The vector is compatible with pBR322 and can be used to study the expression of cloned genes in minicells.
T3 71-93 Microorganism denotes Salmonella typhimurium 90371
T4 141-155 Phenotype denotes drug-resistant OBT:000598
T5 166-194 Microorganism denotes Salmonella typhimurium (993) 90371
T6 256-267 Phenotype denotes wild strain OBT:000169
T7 271-281 Microorganism denotes Salmonella 590
T8 295-298 Microorganism denotes 993 90371
T9 371-391 Phenotype denotes ampicillin-resistant OBT:002762
T10 620-634 Microorganism denotes S. typhimurium 90371
E1 166-194 Exhibits denotes Salmonella typhimurium (993)
E2 271-281 Exhibits denotes Salmonella
R1 E1 T5 Microorganism Salmonella typhimurium (993),Salmonella typhimurium (993)
R2 E1 T4 Property Salmonella typhimurium (993),drug-resistant
R3 E2 T7 Microorganism Salmonella,Salmonella
R4 E2 T6 Property Salmonella,wild strain