BB-kb+ner@ldeleger:BB-kb+ner-12934822 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/BB-kb+ner@ldeleger/sourceid/BB-kb+ner-12934822","sourcedb":"BB-kb+ner@ldeleger","sourceid":"BB-kb+ner-12934822","text":"Detection of Cryptosporidium parvum in secondary effluents using a most probable number-polymerase chain reaction assay.\nPolymerase chain reaction (PCR) was used to detect Cryptosporidium parvum oocysts in secondary effluent samples collected from activated-sludge facilities. Serial dilutions of the purified nucleic acid extracts from the samples were made and PCR was conducted to estimate the C. parvum oocyst concentration via a Poisson distribution-based most probable number (MPN). The degree of oocysts associated with wastewater particles was also evaluated. The sensitivity of the MPN-PCR assay was 20 oocysts/PCR unit. The detection limit of the concentration, extraction, and purification protocols in phosphate buffer saline spiked with a known concentration of oocysts ranged from 1.1 to 4.6 oocysts/L; the detection limit for the wastewater samples ranged from 11 to 4200 oocysts/L depending on the extent of inhibition in each sample. The recovery efficiency of the oocysts ranged from 48 to 59% in most samples. Oocysts were found in two out of seven samples with concentrations of 203 and 308 oocysts/L, as estimated by the MPN-PCR method. The oocysts were found only in the filtrate of the grab samples; particle-associated oocysts were not detected. Association of spiked C. parvum oocysts with particles in secondary effluent drawn from wastewater plants with varying operating conditions indicated a weak correlation between the degree of association and the mean cell residence time of the system.\n\n","tracks":[{"project":"bionlp-ost-19-BB-kb-ner-test","denotations":[{"id":"T1","span":{"begin":0,"end":120},"obj":"Title"},{"id":"T2","span":{"begin":121,"end":1520},"obj":"Paragraph"}],"attributes":[{"subj":"T1","pred":"source","obj":"bionlp-ost-19-BB-kb-ner-test"},{"subj":"T2","pred":"source","obj":"bionlp-ost-19-BB-kb-ner-test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"bionlp-ost-19-BB-kb-ner-test","color":"#93ecd2","default":true}]}]}}