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Id Subject Object Predicate Lexical cue
T1 0-147 Sentence denotes Expression of calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger.
T2 148-368 Sentence denotes The calcineurin B homologous protein (designated CHP1) has been shown to be a common essential cofactor for the plasma membrane Na(+)/H(+) exchangers (NHEs) (Pang, T., Su, X., Wakabayashi, S., and Shigekawa, M. (2001) J.
T3 369-374 Sentence denotes Biol.
T4 375-380 Sentence denotes Chem.
T5 381-399 Sentence denotes 276, 17367-17372).
T6 400-534 Sentence denotes In this study, we characterized the function of another isoform of CHP (designated CHP2) that has a 61% amino acid identity with CHP1.
T7 535-784 Sentence denotes CHP2, like CHP1, conferred the ability to NHEs 1-3 to express a high exchange activity by binding to the juxtamembrane region of the cytoplasmic domain of the exchanger, but it interacts more strongly (approximately 5-fold) with NHE1 than does CHP1.
T8 785-938 Sentence denotes Although CHP1 is expressed ubiquitously at relatively high levels, CHP2 expression was extremely low in most human tissues but was higher in tumor cells.
T9 939-1057 Sentence denotes We produced stable cell clones overexpressing either CHP1 or CHP2 in which one of them is predominantly bound to NHE1.
T10 1058-1219 Sentence denotes Serum (10%) induced a significant cytoplasmic alkalinization (0.1-0.2 pH unit) in cells co-expressing CHP1 and NHE1 but not in cells co-expressing CHP2 and NHE1.
T11 1220-1333 Sentence denotes In the latter, pH(i) was high (7.4-7.5) even in the absence of serum, suggesting that NHE1 was already activated.
T12 1334-1457 Sentence denotes Surprisingly, most (>80%) of CHP2/NHE1 cells unlike CHP1/NHE1 cells were viable even after long serum starvation (>7 days).
T13 1458-1569 Sentence denotes Thus, the expression of CHP2 appears to protect cells from serum deprivation-induced death by increasing pH(i).
T14 1570-1660 Sentence denotes These properties of CHP2/NHE1 cells are similar to those of malignantly transformed cells.
T15 1661-1883 Sentence denotes We propose that serum-independent activation of NHE1 by bound CHP2 is one of the key mechanisms for the maintenance of high pH(i) and the resistance to serum deprivation-induced cell death in malignantly transformed cells.