| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-135 |
Sentence |
denotes |
Role of intronic E- and N-box motifs in the transcriptional induction of the acetylcholinesterase gene during myogenic differentiation. |
| T2 |
136-274 |
Sentence |
denotes |
In this study, we examined whether an intronic N-box motif is involved in the expression of acetylcholinesterase (AChE) during myogenesis. |
| T3 |
275-411 |
Sentence |
denotes |
We determined that AChE transcripts are barely detectable in cultured myoblasts and that their levels increase dramatically in myotubes. |
| T4 |
412-551 |
Sentence |
denotes |
Nuclear run-on assays revealed that this increase was accompanied by a parallel induction in the transcriptional activity of the AChE gene. |
| T5 |
552-675 |
Sentence |
denotes |
These changes in transcription were also observed in transfection experiments using AChE promoter-reporter gene constructs. |
| T6 |
676-809 |
Sentence |
denotes |
Mutation of the intronic N-box at position +755 base pairs (bp) reduced by more than 70% expression of the reporter gene in myotubes. |
| T7 |
810-944 |
Sentence |
denotes |
Disruption of an adjacent E-box, at position +767 bp, also reduced expression of the reporter gene following myogenic differentiation. |
| T8 |
945-1154 |
Sentence |
denotes |
Co-transfection experiments using AChE promoter-reporter gene constructs and a myogenin expression vector showed that expression of this regulatory factor increased expression of the reporter gene in myotubes. |
| T9 |
1155-1323 |
Sentence |
denotes |
Although the AChE promoter contains multiple E-boxes, mutation of this intronic one was sufficient to prevent the myogenin-induced increase in reporter gene expression. |
| T10 |
1324-1556 |
Sentence |
denotes |
Together, these results indicate that changes in AChE gene transcription occur during myogenesis and highlight the contribution of the intronic N- and E-box motifs in the developmental regulation of the AChE gene in skeletal muscle. |
