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PMC:394351 / 17497-18529 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T4909 0-5 JJ denotes Total
T4910 6-15 NNS denotes membranes
T4912 16-20 VBD denotes were
T4911 21-29 VBN denotes prepared
T4913 30-32 IN denotes by
T4914 33-39 VBG denotes taking
T4915 40-53 RB denotes approximately
T4916 54-57 CD denotes 500
T4917 58-60 NN denotes mg
T4918 61-63 IN denotes of
T4919 64-69 NN denotes liver
T4920 70-76 NN denotes tissue
T4921 77-80 VBD denotes cut
T4922 81-85 IN denotes into
T4923 86-91 JJ denotes small
T4924 92-98 NNS denotes pieces
T4925 99-102 CC denotes and
T4926 103-114 VBN denotes homogenized
T4927 115-117 IN denotes in
T4928 118-121 NN denotes ice
T4929 122-126 JJ denotes cold
T4931 127-132 NN denotes lysis
T4930 133-139 NN denotes buffer
T4932 140-141 -LRB- denotes (
T4934 141-142 CD denotes 5
T4935 143-145 NN denotes mM
T4933 146-150 NN denotes Tris
T4936 150-151 HYPH denotes -
T4937 151-153 NN denotes pH
T4938 153-156 CD denotes 7.5
T4939 156-158 , denotes ,
T4940 158-161 CD denotes 250
T4941 162-164 NN denotes mM
T4942 165-172 NN denotes sucrose
T4943 173-177 IN denotes with
T4944 178-186 NN denotes protease
T4945 187-197 NNS denotes inhibitors
T4946 197-198 -RRB- denotes )
T4947 199-201 IN denotes by
T4948 202-203 DT denotes a
T4950 204-210 NN denotes dounce
T4949 211-222 NN denotes homogenizer
T4951 223-228 IN denotes times
T4952 229-231 CD denotes 10
T4953 232-239 NNS denotes strokes
T4954 239-240 . denotes .
T4955 240-318 sentence denotes Samples were then subjected to centrifugation – 1300 g for 10 minutes at 4°C.
T4956 241-248 NNS denotes Samples
T4958 249-253 VBD denotes were
T4959 254-258 RB denotes then
T4957 259-268 VBN denotes subjected
T4960 269-271 IN denotes to
T4961 272-286 NN denotes centrifugation
T4962 287-288 : denotes –
T4963 289-293 CD denotes 1300
T4964 294-295 NN denotes g
T4965 296-299 IN denotes for
T4966 300-302 CD denotes 10
T4967 303-310 NNS denotes minutes
T4968 311-313 IN denotes at
T4969 314-315 CD denotes 4
T4970 315-317 NN denotes °C
T4971 317-318 . denotes .
T4972 318-482 sentence denotes The supernant was saved and the pellet was resuspended and again subjected to dounce homogenization times 10 strokes then centrifuged 1000 g for 10 minutes at 4°C.
T4973 319-322 DT denotes The
T4974 323-332 NN denotes supernant
T4976 333-336 VBD denotes was
T4975 337-342 VBN denotes saved
T4977 343-346 CC denotes and
T4978 347-350 DT denotes the
T4979 351-357 NN denotes pellet
T4981 358-361 VBD denotes was
T4980 362-373 VBN denotes resuspended
T4982 374-377 CC denotes and
T4983 378-383 RB denotes again
T4984 384-393 VBN denotes subjected
T4985 394-396 IN denotes to
T4986 397-403 NN denotes dounce
T4987 404-418 NN denotes homogenization
T4988 419-424 IN denotes times
T4989 425-427 CD denotes 10
T4990 428-435 NNS denotes strokes
T4991 436-440 RB denotes then
T4992 441-452 VBD denotes centrifuged
T4993 453-457 CD denotes 1000
T4994 458-459 NN denotes g
T4995 460-463 IN denotes for
T4996 464-466 CD denotes 10
T4997 467-474 NNS denotes minutes
T4998 475-477 IN denotes at
T4999 478-479 CD denotes 4
T5000 479-481 NN denotes °C
T5001 481-482 . denotes .
T5002 482-573 sentence denotes This step was repeated twice each time with the supernant being collected and kept on ice.
T5003 483-487 DT denotes This
T5004 488-492 NN denotes step
T5006 493-496 VBD denotes was
T5005 497-505 VBN denotes repeated
T5007 506-511 RB denotes twice
T5009 512-516 DT denotes each
T5008 517-521 NN denotes time
T5010 522-526 IN denotes with
T5011 527-530 DT denotes the
T5012 531-540 NN denotes supernant
T5014 541-546 VBG denotes being
T5013 547-556 VBN denotes collected
T5015 557-560 CC denotes and
T5016 561-565 VBN denotes kept
T5017 566-568 IN denotes on
T5018 569-572 NN denotes ice
T5019 572-573 . denotes .
T5020 573-678 sentence denotes The supernants were then pooled and subjected to ultracentrifugation at 100,000 g for 60 minutes at 4°C.
T5021 574-577 DT denotes The
T5022 578-588 NNS denotes supernants
T5024 589-593 VBD denotes were
T5025 594-598 RB denotes then
T5023 599-605 VBN denotes pooled
T5026 606-609 CC denotes and
T5027 610-619 VBN denotes subjected
T5028 620-622 IN denotes to
T5029 623-642 NN denotes ultracentrifugation
T5030 643-645 IN denotes at
T5031 646-653 CD denotes 100,000
T5032 654-655 NN denotes g
T5033 656-659 IN denotes for
T5034 660-662 CD denotes 60
T5035 663-670 NNS denotes minutes
T5036 671-673 IN denotes at
T5037 674-675 CD denotes 4
T5038 675-677 NN denotes °C
T5039 677-678 . denotes .
T5040 678-752 sentence denotes The supernant was removed and the pellet was resuspended in lysis buffer.
T5041 679-682 DT denotes The
T5042 683-692 NN denotes supernant
T5044 693-696 VBD denotes was
T5043 697-704 VBN denotes removed
T5045 705-708 CC denotes and
T5046 709-712 DT denotes the
T5047 713-719 NN denotes pellet
T5049 720-723 VBD denotes was
T5048 724-735 VBN denotes resuspended
T5050 736-738 IN denotes in
T5051 739-744 NN denotes lysis
T5052 745-751 NN denotes buffer
T5053 751-752 . denotes .
T5054 752-813 sentence denotes Plasma membranes were prepared as previously described [31].
T5055 753-759 NN denotes Plasma
T5056 760-769 NNS denotes membranes
T5058 770-774 VBD denotes were
T5057 775-783 VBN denotes prepared
T5059 784-786 IN denotes as
T5061 787-797 RB denotes previously
T5060 798-807 VBN denotes described
T5062 808-809 -LRB- denotes [
T5063 809-811 CD denotes 31
T5064 811-812 -RRB- denotes ]
T5065 812-813 . denotes .
T5066 813-958 sentence denotes Protein sample concentrations were then determined by Bio-Rad protein colormetric assay per manufacturer's protocol (BioRad, Hercules, CA, USA).
T5067 814-821 NN denotes Protein
T5068 822-828 NN denotes sample
T5069 829-843 NNS denotes concentrations
T5071 844-848 VBD denotes were
T5072 849-853 RB denotes then
T5070 854-864 VBN denotes determined
T5073 865-867 IN denotes by
T5074 868-871 NNP denotes Bio
T5076 871-872 HYPH denotes -
T5075 872-875 NNP denotes Rad
T5078 876-883 NN denotes protein
T5079 884-895 JJ denotes colormetric
T5077 896-901 NN denotes assay
T5080 902-905 IN denotes per
T5081 906-918 NN denotes manufacturer
T5083 918-920 POS denotes 's
T5082 921-929 NN denotes protocol
T5084 930-931 -LRB- denotes (
T5085 931-937 NNP denotes BioRad
T5086 937-939 , denotes ,
T5087 939-947 NNP denotes Hercules
T5088 947-949 , denotes ,
T5089 949-951 NNP denotes CA
T5090 951-953 , denotes ,
T5091 953-956 NNP denotes USA
T5092 956-957 -RRB- denotes )
T5093 957-958 . denotes .
T5094 958-1032 sentence denotes 50 μg of membrane proteins were then resolved on 7.5% SDS acrylamide gel.
T5095 959-961 CD denotes 50
T5096 962-964 NN denotes μg
T5098 965-967 IN denotes of
T5099 968-976 NN denotes membrane
T5100 977-985 NN denotes proteins
T5101 986-990 VBD denotes were
T5102 991-995 RB denotes then
T5097 996-1004 VBN denotes resolved
T5103 1005-1007 IN denotes on
T5104 1008-1011 CD denotes 7.5
T5105 1011-1012 NN denotes %
T5107 1013-1016 NN denotes SDS
T5108 1017-1027 NN denotes acrylamide
T5106 1028-1031 NN denotes gel
T5109 1031-1032 . denotes .
R3234 T4909 T4910 amod Total,membranes
R3235 T4910 T4911 nsubjpass membranes,prepared
R3236 T4912 T4911 auxpass were,prepared
R3237 T4913 T4911 prep by,prepared
R3238 T4914 T4913 pcomp taking,by
R3239 T4915 T4916 advmod approximately,500
R3240 T4916 T4917 nummod 500,mg
R3241 T4917 T4914 dobj mg,taking
R3242 T4918 T4917 prep of,mg
R3243 T4919 T4920 compound liver,tissue
R3244 T4920 T4918 pobj tissue,of
R3245 T4921 T4920 acl cut,tissue
R3246 T4922 T4921 prep into,cut
R3247 T4923 T4924 amod small,pieces
R3248 T4924 T4922 pobj pieces,into
R3249 T4925 T4921 cc and,cut
R3250 T4926 T4921 conj homogenized,cut
R3251 T4927 T4926 prep in,homogenized
R3252 T4928 T4929 npadvmod ice,cold
R3253 T4929 T4930 amod cold,buffer
R3254 T4930 T4927 pobj buffer,in
R3255 T4931 T4930 compound lysis,buffer
R3256 T4932 T4933 punct (,Tris
R3257 T4933 T4930 parataxis Tris,buffer
R3258 T4934 T4935 nummod 5,mM
R3259 T4935 T4933 compound mM,Tris
R3260 T4936 T4933 punct -,Tris
R3261 T4937 T4933 npadvmod pH,Tris
R3262 T4938 T4937 nummod 7.5,pH
R3263 T4939 T4933 punct ", ",Tris
R3264 T4940 T4941 nummod 250,mM
R3265 T4941 T4942 compound mM,sucrose
R3266 T4942 T4933 appos sucrose,Tris
R3267 T4943 T4933 prep with,Tris
R3268 T4944 T4945 compound protease,inhibitors
R3269 T4945 T4943 pobj inhibitors,with
R3270 T4946 T4933 punct ),Tris
R3271 T4947 T4926 agent by,homogenized
R3272 T4948 T4949 det a,homogenizer
R3273 T4949 T4947 pobj homogenizer,by
R3274 T4950 T4949 compound dounce,homogenizer
R3275 T4951 T4949 prep times,homogenizer
R3276 T4952 T4953 nummod 10,strokes
R3277 T4953 T4951 pobj strokes,times
R3278 T4954 T4911 punct .,prepared
R3279 T4956 T4957 nsubjpass Samples,subjected
R3280 T4958 T4957 auxpass were,subjected
R3281 T4959 T4957 advmod then,subjected
R3282 T4960 T4957 prep to,subjected
R3283 T4961 T4960 pobj centrifugation,to
R3284 T4962 T4961 punct –,centrifugation
R3285 T4963 T4964 nummod 1300,g
R3286 T4964 T4961 npadvmod g,centrifugation
R3287 T4965 T4964 prep for,g
R3288 T4966 T4967 nummod 10,minutes
R3289 T4967 T4965 pobj minutes,for
R3290 T4968 T4964 prep at,g
R3291 T4969 T4970 nummod 4,°C
R3292 T4970 T4968 pobj °C,at
R3293 T4971 T4957 punct .,subjected
R3294 T4973 T4974 det The,supernant
R3295 T4974 T4975 nsubjpass supernant,saved
R3296 T4976 T4975 auxpass was,saved
R3297 T4977 T4975 cc and,saved
R3298 T4978 T4979 det the,pellet
R3299 T4979 T4980 nsubjpass pellet,resuspended
R3300 T4980 T4975 conj resuspended,saved
R3301 T4981 T4980 auxpass was,resuspended
R3302 T4982 T4980 cc and,resuspended
R3303 T4983 T4984 advmod again,subjected
R3304 T4984 T4980 conj subjected,resuspended
R3305 T4985 T4984 prep to,subjected
R3306 T4986 T4987 compound dounce,homogenization
R3307 T4987 T4985 pobj homogenization,to
R3308 T4988 T4987 prep times,homogenization
R3309 T4989 T4990 nummod 10,strokes
R3310 T4990 T4988 pobj strokes,times
R3311 T4991 T4992 advmod then,centrifuged
R3312 T4992 T4984 dep centrifuged,subjected
R3313 T4993 T4994 nummod 1000,g
R3314 T4994 T4992 npadvmod g,centrifuged
R3315 T4995 T4992 prep for,centrifuged
R3316 T4996 T4997 nummod 10,minutes
R3317 T4997 T4995 pobj minutes,for
R3318 T4998 T4992 prep at,centrifuged
R3319 T4999 T5000 nummod 4,°C
R3320 T5000 T4998 pobj °C,at
R3321 T5001 T4980 punct .,resuspended
R3322 T5003 T5004 det This,step
R3323 T5004 T5005 nsubjpass step,repeated
R3324 T5006 T5005 auxpass was,repeated
R3325 T5007 T5008 advmod twice,time
R3326 T5008 T5005 npadvmod time,repeated
R3327 T5009 T5008 det each,time
R3328 T5010 T5005 prep with,repeated
R3329 T5011 T5012 det the,supernant
R3330 T5012 T5013 nsubjpass supernant,collected
R3331 T5013 T5010 pcomp collected,with
R3332 T5014 T5013 auxpass being,collected
R3333 T5015 T5013 cc and,collected
R3334 T5016 T5013 conj kept,collected
R3335 T5017 T5016 oprd on,kept
R3336 T5018 T5017 pobj ice,on
R3337 T5019 T5005 punct .,repeated
R3338 T5021 T5022 det The,supernants
R3339 T5022 T5023 nsubjpass supernants,pooled
R3340 T5024 T5023 auxpass were,pooled
R3341 T5025 T5023 advmod then,pooled
R3342 T5026 T5023 cc and,pooled
R3343 T5027 T5023 conj subjected,pooled
R3344 T5028 T5027 prep to,subjected
R3345 T5029 T5028 pobj ultracentrifugation,to
R3346 T5030 T5029 prep at,ultracentrifugation
R3347 T5031 T5032 nummod "100,000",g
R3348 T5032 T5030 pobj g,at
R3349 T5033 T5027 prep for,subjected
R3350 T5034 T5035 nummod 60,minutes
R3351 T5035 T5033 pobj minutes,for
R3352 T5036 T5027 prep at,subjected
R3353 T5037 T5038 nummod 4,°C
R3354 T5038 T5036 pobj °C,at
R3355 T5039 T5023 punct .,pooled
R3356 T5041 T5042 det The,supernant
R3357 T5042 T5043 nsubjpass supernant,removed
R3358 T5044 T5043 auxpass was,removed
R3359 T5045 T5043 cc and,removed
R3360 T5046 T5047 det the,pellet
R3361 T5047 T5048 nsubjpass pellet,resuspended
R3362 T5048 T5043 conj resuspended,removed
R3363 T5049 T5048 auxpass was,resuspended
R3364 T5050 T5048 prep in,resuspended
R3365 T5051 T5052 compound lysis,buffer
R3366 T5052 T5050 pobj buffer,in
R3367 T5053 T5048 punct .,resuspended
R3368 T5055 T5056 compound Plasma,membranes
R3369 T5056 T5057 nsubjpass membranes,prepared
R3370 T5058 T5057 auxpass were,prepared
R3371 T5059 T5060 mark as,described
R3372 T5060 T5057 advcl described,prepared
R3373 T5061 T5060 advmod previously,described
R3374 T5062 T5063 punct [,31
R3375 T5063 T5057 parataxis 31,prepared
R3376 T5064 T5063 punct ],31
R3377 T5065 T5057 punct .,prepared
R3378 T5067 T5068 compound Protein,sample
R3379 T5068 T5069 compound sample,concentrations
R3380 T5069 T5070 nsubjpass concentrations,determined
R3381 T5071 T5070 auxpass were,determined
R3382 T5072 T5070 advmod then,determined
R3383 T5073 T5070 agent by,determined
R3384 T5074 T5075 nmod Bio,Rad
R3385 T5075 T5077 nmod Rad,assay
R3386 T5076 T5075 punct -,Rad
R3387 T5077 T5073 pobj assay,by
R3388 T5078 T5079 npadvmod protein,colormetric
R3389 T5079 T5077 amod colormetric,assay
R3390 T5080 T5070 prep per,determined
R3391 T5081 T5082 poss manufacturer,protocol
R3392 T5082 T5080 pobj protocol,per
R3393 T5083 T5081 case 's,manufacturer
R3394 T5084 T5085 punct (,BioRad
R3395 T5085 T5082 parataxis BioRad,protocol
R3396 T5086 T5085 punct ", ",BioRad
R3397 T5087 T5085 npadvmod Hercules,BioRad
R3398 T5088 T5085 punct ", ",BioRad
R3399 T5089 T5085 npadvmod CA,BioRad
R3400 T5090 T5085 punct ", ",BioRad
R3401 T5091 T5085 npadvmod USA,BioRad
R3402 T5092 T5085 punct ),BioRad
R3403 T5093 T5070 punct .,determined
R3404 T5095 T5096 nummod 50,μg
R3405 T5096 T5097 nsubjpass μg,resolved
R3406 T5098 T5096 prep of,μg
R3407 T5099 T5100 compound membrane,proteins
R3408 T5100 T5098 pobj proteins,of
R3409 T5101 T5097 auxpass were,resolved
R3410 T5102 T5097 advmod then,resolved
R3411 T5103 T5097 prep on,resolved
R3412 T5104 T5105 nummod 7.5,%
R3413 T5105 T5106 compound %,gel
R3414 T5106 T5103 pobj gel,on
R3415 T5107 T5106 compound SDS,gel
R3416 T5108 T5106 compound acrylamide,gel
R3417 T5109 T5097 punct .,resolved