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PMC:194730 / 11478-15378 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE-old TextAE

Id Subject Object Predicate Lexical cue
T3280 0-3 DT denotes The
T3281 4-8 NN denotes lack
T3283 9-11 IN denotes of
T3284 12-19 NN denotes annexin
T3285 20-22 NN denotes A7
T3282 23-30 VBZ denotes changes
T3286 31-34 JJ denotes red
T3288 35-40 NN denotes blood
T3287 41-45 NN denotes cell
T3289 46-56 NN denotes morphology
T3290 57-60 CC denotes and
T3291 61-68 JJ denotes osmotic
T3292 69-79 NN denotes resistance
T3293 79-265 sentence denotes As annexin A7 is a component of red blood cell vesicles we studied the consequences of the loss of the protein in the anxA7-/-mouse for red blood cell morphology and osmotic resistance.
T3294 80-82 IN denotes As
T3296 83-90 NN denotes annexin
T3297 91-93 NN denotes A7
T3295 94-96 VBZ denotes is
T3299 97-98 DT denotes a
T3300 99-108 NN denotes component
T3301 109-111 IN denotes of
T3302 112-115 JJ denotes red
T3304 116-121 NN denotes blood
T3303 122-126 NN denotes cell
T3305 127-135 NNS denotes vesicles
T3306 136-138 PRP denotes we
T3298 139-146 VBD denotes studied
T3307 147-150 DT denotes the
T3308 151-163 NNS denotes consequences
T3309 164-166 IN denotes of
T3310 167-170 DT denotes the
T3311 171-175 NN denotes loss
T3312 176-178 IN denotes of
T3313 179-182 DT denotes the
T3314 183-190 NN denotes protein
T3315 191-193 IN denotes in
T3316 194-197 DT denotes the
T3318 198-203 NN denotes anxA7
T3319 203-204 SYM denotes -
T3320 204-205 HYPH denotes /
T3321 205-206 SYM denotes -
T3317 206-211 NN denotes mouse
T3322 212-215 IN denotes for
T3323 216-219 JJ denotes red
T3325 220-225 NN denotes blood
T3324 226-230 NN denotes cell
T3326 231-241 NN denotes morphology
T3327 242-245 CC denotes and
T3328 246-253 JJ denotes osmotic
T3329 254-264 NN denotes resistance
T3330 264-265 . denotes .
T3331 265-438 sentence denotes In a standard 'blood smear' we did not note a deformation of the cells, however dark field microscopy revealed changes in shape and diameter of the anxA7-/-red blood cells.
T3332 266-268 IN denotes In
T3334 269-270 DT denotes a
T3336 271-279 JJ denotes standard
T3337 280-281 `` denotes '
T3338 281-286 NN denotes blood
T3335 287-292 NN denotes smear
T3339 292-293 '' denotes '
T3340 294-296 PRP denotes we
T3342 297-300 VBD denotes did
T3343 301-304 RB denotes not
T3341 305-309 VB denotes note
T3344 310-311 DT denotes a
T3345 312-323 NN denotes deformation
T3346 324-326 IN denotes of
T3347 327-330 DT denotes the
T3348 331-336 NNS denotes cells
T3349 336-338 , denotes ,
T3350 338-345 RB denotes however
T3351 346-350 JJ denotes dark
T3352 351-356 NN denotes field
T3353 357-367 NN denotes microscopy
T3333 368-376 VBD denotes revealed
T3354 377-384 NNS denotes changes
T3355 385-387 IN denotes in
T3356 388-393 NN denotes shape
T3357 394-397 CC denotes and
T3358 398-406 NN denotes diameter
T3359 407-409 IN denotes of
T3360 410-413 DT denotes the
T3362 414-419 JJ denotes anxA7
T3363 419-420 SYM denotes -
T3364 420-421 HYPH denotes /
T3365 421-422 SYM denotes -
T3366 422-425 JJ denotes red
T3367 426-431 NN denotes blood
T3361 432-437 NNS denotes cells
T3368 437-438 . denotes .
T3369 438-642 sentence denotes They had a statistically significant larger cell diameter of 6.0 μm compared to wild type with 5.7 μm (p = 0.015, n = 40), a remarkably lower emphasized central impression and a more flat shape (Fig. 4).
T3370 439-443 PRP denotes They
T3371 444-447 VBD denotes had
T3372 448-449 DT denotes a
T3374 450-463 RB denotes statistically
T3375 464-475 JJ denotes significant
T3376 476-482 JJR denotes larger
T3377 483-487 NN denotes cell
T3373 488-496 NN denotes diameter
T3378 497-499 IN denotes of
T3379 500-503 CD denotes 6.0
T3380 504-506 NN denotes μm
T3381 507-515 VBN denotes compared
T3382 516-518 IN denotes to
T3383 519-523 JJ denotes wild
T3384 524-528 NN denotes type
T3385 529-533 IN denotes with
T3386 534-537 CD denotes 5.7
T3387 538-540 NNS denotes μm
T3388 541-542 -LRB- denotes (
T3390 542-543 NN denotes p
T3392 544-545 SYM denotes =
T3391 546-551 CD denotes 0.015
T3393 551-553 , denotes ,
T3394 553-554 NN denotes n
T3395 555-556 SYM denotes =
T3389 557-559 CD denotes 40
T3396 559-560 -RRB- denotes )
T3397 560-562 , denotes ,
T3398 562-563 DT denotes a
T3400 564-574 RB denotes remarkably
T3401 575-580 JJR denotes lower
T3402 581-591 VBN denotes emphasized
T3403 592-599 JJ denotes central
T3399 600-610 NN denotes impression
T3404 611-614 CC denotes and
T3405 615-616 DT denotes a
T3407 617-621 RBR denotes more
T3408 622-626 JJ denotes flat
T3406 627-632 NN denotes shape
T3409 633-634 -LRB- denotes (
T3410 634-638 NN denotes Fig.
T3411 639-640 CD denotes 4
T3412 640-641 -RRB- denotes )
T3413 641-642 . denotes .
T3414 642-747 sentence denotes No significant change in the mean corpuscular volume (MCV) was measured with the ADVIA 120 cell counter.
T3415 643-645 DT denotes No
T3417 646-657 JJ denotes significant
T3416 658-664 NN denotes change
T3419 665-667 IN denotes in
T3420 668-671 DT denotes the
T3422 672-676 JJ denotes mean
T3423 677-688 JJ denotes corpuscular
T3421 689-695 NN denotes volume
T3424 696-697 -LRB- denotes (
T3425 697-700 NN denotes MCV
T3426 700-701 -RRB- denotes )
T3427 702-705 VBD denotes was
T3418 706-714 VBN denotes measured
T3428 715-719 IN denotes with
T3429 720-723 DT denotes the
T3431 724-729 NN denotes ADVIA
T3432 730-733 CD denotes 120
T3433 734-738 NN denotes cell
T3430 739-746 NN denotes counter
T3434 746-747 . denotes .
T3435 747-748 sentence denotes
T9489 758-762 JJ denotes Dark
T9490 763-768 NN denotes field
T9491 769-779 NN denotes microscopy
T9492 780-782 IN denotes of
T9493 783-786 JJ denotes red
T9495 787-792 NN denotes blood
T9494 793-798 NNS denotes cells
T9496 799-803 IN denotes from
T9497 804-809 JJ denotes anxA7
T9499 809-810 SYM denotes -
T9500 810-811 HYPH denotes /
T9501 811-812 SYM denotes -
T9498 813-819 NN denotes mutant
T9503 820-821 -LRB- denotes (
T9504 821-822 NN denotes B
T9505 822-823 -RRB- denotes )
T9506 824-827 CC denotes and
T9507 828-832 JJ denotes wild
T9508 833-837 NN denotes type
T9502 838-842 NNS denotes mice
T9509 843-844 -LRB- denotes (
T9510 844-845 NN denotes A
T9511 845-846 -RRB- denotes )
T9512 846-847 . denotes .
T9513 847-912 sentence denotes The mean cellular diameters are 6.0 μm and 5.7 μm, respectively.
T9514 848-851 DT denotes The
T9516 852-856 JJ denotes mean
T9517 857-865 JJ denotes cellular
T9515 866-875 NNS denotes diameters
T9518 876-879 VBP denotes are
T9519 880-883 CD denotes 6.0
T9520 884-886 NNS denotes μm
T9521 887-890 CC denotes and
T9522 891-894 CD denotes 5.7
T9523 895-897 NNS denotes μm
T9524 897-899 , denotes ,
T9525 899-911 RB denotes respectively
T9526 911-912 . denotes .
T9527 912-984 sentence denotes The size differences are statistically significant (p = 0.015; n = 40).
T9528 913-916 DT denotes The
T9530 917-921 NN denotes size
T9529 922-933 NNS denotes differences
T9531 934-937 VBP denotes are
T9532 938-951 RB denotes statistically
T9533 952-963 JJ denotes significant
T9534 964-965 -LRB- denotes (
T9536 965-966 NN denotes p
T9538 967-968 SYM denotes =
T9537 969-974 CD denotes 0.015
T9539 974-975 : denotes ;
T9540 976-977 NN denotes n
T9541 978-979 SYM denotes =
T9535 980-982 CD denotes 40
T9542 982-983 -RRB- denotes )
T9543 983-984 . denotes .
T9544 984-995 sentence denotes Bar, 3 μm.
T9545 985-988 NN denotes Bar
T9547 988-990 , denotes ,
T9548 990-991 CD denotes 3
T9546 992-994 NNS denotes μm
T9549 994-995 . denotes .
T3437 996-1001 NN denotes Shape
T3436 996-1087 sentence denotes Shape and MCV of a red blood cell essentially influence its function and capillary passage.
T3439 1002-1005 CC denotes and
T3440 1006-1009 NN denotes MCV
T3441 1010-1012 IN denotes of
T3442 1013-1014 DT denotes a
T3444 1015-1018 JJ denotes red
T3445 1019-1024 NN denotes blood
T3443 1025-1029 NN denotes cell
T3446 1030-1041 RB denotes essentially
T3438 1042-1051 VB denotes influence
T3447 1052-1055 PRP$ denotes its
T3448 1056-1064 NN denotes function
T3449 1065-1068 CC denotes and
T3450 1069-1078 JJ denotes capillary
T3451 1079-1086 NN denotes passage
T3452 1086-1087 . denotes .
T3453 1087-1261 sentence denotes The typical biconcave form depends on various influences including the membrane lipid composition and the submembranous cytoskeleton [32] where annexin A7 might play a role.
T3454 1088-1091 DT denotes The
T3456 1092-1099 JJ denotes typical
T3457 1100-1109 NN denotes biconcave
T3455 1110-1114 NN denotes form
T3458 1115-1122 VBZ denotes depends
T3459 1123-1125 IN denotes on
T3460 1126-1133 JJ denotes various
T3461 1134-1144 NNS denotes influences
T3462 1145-1154 VBG denotes including
T3463 1155-1158 DT denotes the
T3465 1159-1167 NN denotes membrane
T3466 1168-1173 NN denotes lipid
T3464 1174-1185 NN denotes composition
T3467 1186-1189 CC denotes and
T3468 1190-1193 DT denotes the
T3470 1194-1207 JJ denotes submembranous
T3469 1208-1220 NN denotes cytoskeleton
T3471 1221-1222 -LRB- denotes [
T3472 1222-1224 CD denotes 32
T3473 1224-1225 -RRB- denotes ]
T3474 1226-1231 WRB denotes where
T3476 1232-1239 NN denotes annexin
T3477 1240-1242 NN denotes A7
T3478 1243-1248 MD denotes might
T3475 1249-1253 VB denotes play
T3479 1254-1255 DT denotes a
T3480 1256-1260 NN denotes role
T3481 1260-1261 . denotes .
T3482 1261-1430 sentence denotes The osmotic resistance, which is the resistance towards changes in the extracellular ionic strength, is a convenient assay for analysis of the red blood cell integrity.
T3483 1262-1265 DT denotes The
T3485 1266-1273 JJ denotes osmotic
T3484 1274-1284 NN denotes resistance
T3487 1284-1286 , denotes ,
T3488 1286-1291 WDT denotes which
T3489 1292-1294 VBZ denotes is
T3490 1295-1298 DT denotes the
T3491 1299-1309 NN denotes resistance
T3492 1310-1317 IN denotes towards
T3493 1318-1325 NNS denotes changes
T3494 1326-1328 IN denotes in
T3495 1329-1332 DT denotes the
T3497 1333-1346 JJ denotes extracellular
T3498 1347-1352 JJ denotes ionic
T3496 1353-1361 NN denotes strength
T3499 1361-1363 , denotes ,
T3486 1363-1365 VBZ denotes is
T3500 1366-1367 DT denotes a
T3502 1368-1378 JJ denotes convenient
T3501 1379-1384 NN denotes assay
T3503 1385-1388 IN denotes for
T3504 1389-1397 NN denotes analysis
T3505 1398-1400 IN denotes of
T3506 1401-1404 DT denotes the
T3508 1405-1408 JJ denotes red
T3510 1409-1414 NN denotes blood
T3509 1415-1419 NN denotes cell
T3507 1420-1429 NN denotes integrity
T3511 1429-1430 . denotes .
T3512 1430-1597 sentence denotes It is measured as the sodium chloride concentration at which cellular lysis starts (minimal resistance) up to a complete lysis (maximal resistance) of the cells [33].
T3513 1431-1433 PRP denotes It
T3515 1434-1436 VBZ denotes is
T3514 1437-1445 VBN denotes measured
T3516 1446-1448 IN denotes as
T3517 1449-1452 DT denotes the
T3519 1453-1459 NN denotes sodium
T3520 1460-1468 NN denotes chloride
T3518 1469-1482 NN denotes concentration
T3521 1483-1485 IN denotes at
T3523 1486-1491 WDT denotes which
T3524 1492-1500 JJ denotes cellular
T3525 1501-1506 NN denotes lysis
T3522 1507-1513 VBZ denotes starts
T3526 1514-1515 -LRB- denotes (
T3527 1515-1522 JJ denotes minimal
T3528 1523-1533 NN denotes resistance
T3529 1533-1534 -RRB- denotes )
T3530 1535-1537 IN denotes up
T3531 1538-1540 IN denotes to
T3532 1541-1542 DT denotes a
T3534 1543-1551 JJ denotes complete
T3533 1552-1557 NN denotes lysis
T3535 1558-1559 -LRB- denotes (
T3536 1559-1566 JJ denotes maximal
T3537 1567-1577 NN denotes resistance
T3538 1577-1578 -RRB- denotes )
T3539 1579-1581 IN denotes of
T3540 1582-1585 DT denotes the
T3541 1586-1591 NNS denotes cells
T3542 1592-1593 -LRB- denotes [
T3543 1593-1595 CD denotes 33
T3544 1595-1596 -RRB- denotes ]
T3545 1596-1597 . denotes .
T3546 1597-1748 sentence denotes Osmotic resistance increases with higher MCV, larger surface area and a higher degree of cellular metabolism stabilizing the intracellular ion levels.
T3547 1598-1605 JJ denotes Osmotic
T3548 1606-1616 NN denotes resistance
T3549 1617-1626 VBZ denotes increases
T3550 1627-1631 IN denotes with
T3551 1632-1638 JJR denotes higher
T3552 1639-1642 NN denotes MCV
T3553 1642-1644 , denotes ,
T3554 1644-1650 JJR denotes larger
T3556 1651-1658 NN denotes surface
T3555 1659-1663 NN denotes area
T3557 1664-1667 CC denotes and
T3558 1668-1669 DT denotes a
T3560 1670-1676 JJR denotes higher
T3559 1677-1683 NN denotes degree
T3561 1684-1686 IN denotes of
T3562 1687-1695 JJ denotes cellular
T3563 1696-1706 NN denotes metabolism
T3564 1707-1718 VBG denotes stabilizing
T3565 1719-1722 DT denotes the
T3567 1723-1736 JJ denotes intracellular
T3568 1737-1740 NN denotes ion
T3566 1741-1747 NNS denotes levels
T3569 1747-1748 . denotes .
T3570 1748-1823 sentence denotes Aged red blood cells show a lowered MCV and lower osmotic resistance [33].
T3571 1749-1753 VBN denotes Aged
T3573 1754-1757 JJ denotes red
T3574 1758-1763 NN denotes blood
T3572 1764-1769 NNS denotes cells
T3575 1770-1774 VBP denotes show
T3576 1775-1776 DT denotes a
T3578 1777-1784 JJ denotes lowered
T3577 1785-1788 NN denotes MCV
T3579 1789-1792 CC denotes and
T3580 1793-1798 JJR denotes lower
T3582 1799-1806 JJ denotes osmotic
T3581 1807-1817 NN denotes resistance
T3583 1818-1819 -LRB- denotes [
T3584 1819-1821 CD denotes 33
T3585 1821-1822 -RRB- denotes ]
T3586 1822-1823 . denotes .
T3587 1823-1913 sentence denotes Similar properties can be observed in red blood cells with lowered membrane permeability.
T3588 1824-1831 JJ denotes Similar
T3589 1832-1842 NNS denotes properties
T3591 1843-1846 MD denotes can
T3592 1847-1849 VB denotes be
T3590 1850-1858 VBN denotes observed
T3593 1859-1861 IN denotes in
T3594 1862-1865 JJ denotes red
T3596 1866-1871 NN denotes blood
T3595 1872-1877 NNS denotes cells
T3597 1878-1882 IN denotes with
T3598 1883-1890 VBN denotes lowered
T3600 1891-1899 NN denotes membrane
T3599 1900-1912 NN denotes permeability
T3601 1912-1913 . denotes .
T3602 1913-2032 sentence denotes The osmotic resistance of annexin A7 deficient red blood cells is significantly increased compared to wild type cells.
T3603 1914-1917 DT denotes The
T3605 1918-1925 JJ denotes osmotic
T3604 1926-1936 NN denotes resistance
T3607 1937-1939 IN denotes of
T3608 1940-1947 NN denotes annexin
T3609 1948-1950 NN denotes A7
T3610 1951-1960 JJ denotes deficient
T3612 1961-1964 JJ denotes red
T3613 1965-1970 NN denotes blood
T3611 1971-1976 NNS denotes cells
T3606 1977-1979 VBZ denotes is
T3614 1980-1993 RB denotes significantly
T3615 1994-2003 VBN denotes increased
T3616 2004-2012 VBN denotes compared
T3617 2013-2015 IN denotes to
T3618 2016-2020 JJ denotes wild
T3619 2021-2025 NN denotes type
T3620 2026-2031 NNS denotes cells
T3621 2031-2032 . denotes .
T3622 2032-2153 sentence denotes The minimal resistance value of knock out cells is observed in a 0.60 % NaCl solution versus 0.65 % for wild type cells.
T3623 2033-2036 DT denotes The
T3625 2037-2044 JJ denotes minimal
T3626 2045-2055 NN denotes resistance
T3624 2056-2061 NN denotes value
T3628 2062-2064 IN denotes of
T3629 2065-2070 NN denotes knock
T3630 2071-2074 NN denotes out
T3631 2075-2080 NNS denotes cells
T3632 2081-2083 VBZ denotes is
T3627 2084-2092 VBN denotes observed
T3633 2093-2095 IN denotes in
T3634 2096-2097 DT denotes a
T3636 2098-2102 CD denotes 0.60
T3637 2103-2104 NN denotes %
T3638 2105-2109 NN denotes NaCl
T3635 2110-2118 NN denotes solution
T3639 2119-2125 CC denotes versus
T3640 2126-2130 CD denotes 0.65
T3641 2131-2132 NN denotes %
T3642 2133-2136 IN denotes for
T3643 2137-2141 JJ denotes wild
T3644 2142-2146 NN denotes type
T3645 2147-2152 NNS denotes cells
T3646 2152-2153 . denotes .
T3647 2153-2303 sentence denotes 50 % haemolysis is achieved at 0.516 % NaCl solution for knock out versus 0.564 % NaCl for wild type cells (mean values, p = 0.00066, n = 8; Fig. 5).
T3648 2154-2156 CD denotes 50
T3649 2157-2158 NN denotes %
T3650 2159-2169 NN denotes haemolysis
T3652 2170-2172 VBZ denotes is
T3651 2173-2181 VBN denotes achieved
T3653 2182-2184 IN denotes at
T3654 2185-2190 CD denotes 0.516
T3655 2191-2192 NN denotes %
T3657 2193-2197 NN denotes NaCl
T3656 2198-2206 NN denotes solution
T3658 2207-2210 IN denotes for
T3659 2211-2216 NN denotes knock
T3660 2217-2220 NN denotes out
T3661 2221-2227 CC denotes versus
T3662 2228-2233 CD denotes 0.564
T3663 2234-2235 NN denotes %
T3664 2236-2240 NN denotes NaCl
T3665 2241-2244 IN denotes for
T3666 2245-2249 JJ denotes wild
T3667 2250-2254 NN denotes type
T3668 2255-2260 NNS denotes cells
T3669 2261-2262 -LRB- denotes (
T3671 2262-2266 NN denotes mean
T3672 2267-2273 NNS denotes values
T3673 2273-2275 , denotes ,
T3674 2275-2276 NN denotes p
T3676 2277-2278 SYM denotes =
T3675 2279-2286 CD denotes 0.00066
T3677 2286-2288 , denotes ,
T3678 2288-2289 NN denotes n
T3680 2290-2291 SYM denotes =
T3679 2292-2293 CD denotes 8
T3681 2293-2294 : denotes ;
T3670 2295-2299 NN denotes Fig.
T3682 2300-2301 CD denotes 5
T3683 2301-2302 -RRB- denotes )
T3684 2302-2303 . denotes .
T3685 2303-2407 sentence denotes The resistance width of the knock out cells is slightly lower (0.20 % instead of 0.25 % NaCl solution).
T3686 2304-2307 DT denotes The
T3688 2308-2318 NN denotes resistance
T3687 2319-2324 NN denotes width
T3690 2325-2327 IN denotes of
T3691 2328-2331 DT denotes the
T3693 2332-2337 NN denotes knock
T3694 2338-2341 NN denotes out
T3692 2342-2347 NNS denotes cells
T3689 2348-2350 VBZ denotes is
T3695 2351-2359 RB denotes slightly
T3696 2360-2365 JJR denotes lower
T3697 2366-2367 -LRB- denotes (
T3699 2367-2371 CD denotes 0.20
T3700 2372-2373 NN denotes %
T3702 2374-2381 RB denotes instead
T3703 2382-2384 IN denotes of
T3704 2385-2389 CD denotes 0.25
T3705 2390-2391 NN denotes %
T3701 2392-2396 NN denotes NaCl
T3698 2397-2405 NN denotes solution
T3706 2405-2406 -RRB- denotes )
T3707 2406-2407 . denotes .
T3708 2407-2572 sentence denotes We also performed direct measurements of the red blood cell membrane deformability, to further characterize a possible role of annexin A7 on the membrane stability.
T3709 2408-2410 PRP denotes We
T3711 2411-2415 RB denotes also
T3710 2416-2425 VBD denotes performed
T3712 2426-2432 JJ denotes direct
T3713 2433-2445 NNS denotes measurements
T3714 2446-2448 IN denotes of
T3715 2449-2452 DT denotes the
T3717 2453-2456 JJ denotes red
T3719 2457-2462 NN denotes blood
T3718 2463-2467 NN denotes cell
T3720 2468-2476 NN denotes membrane
T3716 2477-2490 NN denotes deformability
T3721 2490-2492 , denotes ,
T3722 2492-2494 TO denotes to
T3724 2495-2502 RBR denotes further
T3723 2503-2515 VB denotes characterize
T3725 2516-2517 DT denotes a
T3727 2518-2526 JJ denotes possible
T3726 2527-2531 NN denotes role
T3728 2532-2534 IN denotes of
T3729 2535-2542 NN denotes annexin
T3730 2543-2545 NN denotes A7
T3731 2546-2548 IN denotes on
T3732 2549-2552 DT denotes the
T3734 2553-2561 NN denotes membrane
T3733 2562-2571 NN denotes stability
T3735 2571-2572 . denotes .
T3736 2572-2697 sentence denotes With micropipette experiments we tried to correlate mechanical characteristics of the red blood cells with their morphology.
T3737 2573-2577 IN denotes With
T3739 2578-2590 NN denotes micropipette
T3740 2591-2602 NNS denotes experiments
T3741 2603-2605 PRP denotes we
T3738 2606-2611 VBD denotes tried
T3742 2612-2614 TO denotes to
T3743 2615-2624 VB denotes correlate
T3744 2625-2635 JJ denotes mechanical
T3745 2636-2651 NNS denotes characteristics
T3746 2652-2654 IN denotes of
T3747 2655-2658 DT denotes the
T3749 2659-2662 JJ denotes red
T3750 2663-2668 NN denotes blood
T3748 2669-2674 NNS denotes cells
T3751 2675-2679 IN denotes with
T3752 2680-2685 PRP$ denotes their
T3753 2686-2696 NN denotes morphology
T3754 2696-2697 . denotes .
T3755 2697-2894 sentence denotes We could not observe any statistically significant difference of values describing membrane rigidity and lysis force in these experiments (M. Heil, B. Hoffmann and R. Merkel, unpublished results).
T3756 2698-2700 PRP denotes We
T3758 2701-2706 MD denotes could
T3759 2707-2710 RB denotes not
T3757 2711-2718 VB denotes observe
T3760 2719-2722 DT denotes any
T3762 2723-2736 RB denotes statistically
T3763 2737-2748 JJ denotes significant
T3761 2749-2759 NN denotes difference
T3764 2760-2762 IN denotes of
T3765 2763-2769 NNS denotes values
T3766 2770-2780 VBG denotes describing
T3767 2781-2789 NN denotes membrane
T3768 2790-2798 NN denotes rigidity
T3769 2799-2802 CC denotes and
T3770 2803-2808 NN denotes lysis
T3771 2809-2814 NN denotes force
T3772 2815-2817 IN denotes in
T3773 2818-2823 DT denotes these
T3774 2824-2835 NNS denotes experiments
T3775 2836-2837 -LRB- denotes (
T3776 2837-2839 NNP denotes M.
T3777 2840-2844 NNP denotes Heil
T3778 2844-2846 , denotes ,
T3779 2846-2848 NNP denotes B.
T3780 2849-2857 NNP denotes Hoffmann
T3781 2858-2861 CC denotes and
T3782 2862-2864 NNP denotes R.
T3783 2865-2871 NNP denotes Merkel
T3784 2871-2873 , denotes ,
T3785 2873-2884 JJ denotes unpublished
T3786 2885-2892 NNS denotes results
T3787 2892-2893 -RRB- denotes )
T3788 2893-2894 . denotes .
T3789 2894-3064 sentence denotes However, the distinction observed in the osmotic resistance experiments was highly significant and reflects a mean value over a high number of different red blood cells.
T3790 2895-2902 RB denotes However
T3792 2902-2904 , denotes ,
T3793 2904-2907 DT denotes the
T3794 2908-2919 NN denotes distinction
T3795 2920-2928 VBN denotes observed
T3796 2929-2931 IN denotes in
T3797 2932-2935 DT denotes the
T3799 2936-2943 JJ denotes osmotic
T3800 2944-2954 NN denotes resistance
T3798 2955-2966 NNS denotes experiments
T3791 2967-2970 VBD denotes was
T3801 2971-2977 RB denotes highly
T3802 2978-2989 JJ denotes significant
T3803 2990-2993 CC denotes and
T3804 2994-3002 VBZ denotes reflects
T3805 3003-3004 DT denotes a
T3807 3005-3009 JJ denotes mean
T3806 3010-3015 NN denotes value
T3808 3016-3020 IN denotes over
T3809 3021-3022 DT denotes a
T3811 3023-3027 JJ denotes high
T3810 3028-3034 NN denotes number
T3812 3035-3037 IN denotes of
T3813 3038-3047 JJ denotes different
T3815 3048-3051 JJ denotes red
T3816 3052-3057 NN denotes blood
T3814 3058-3063 NNS denotes cells
T3817 3063-3064 . denotes .
T3818 3064-3722 sentence denotes Figure 5 Osmotic resistance curves of red blood cells from wild type and anxA7-/- mutant mice. The osmotic resistance towards changes in the extracellular ionic strength is a convenient assay for analysis of the red blood cell integrity. It is measured as a sodium chloride concentration in which cellular lysis starts (minimal resistance) up to a complete lysis (maximal resistance) of a red blood cell. The osmotic resistance of annexin A7 deficient red blood cells is significantly increased compared to the one of wild type (p = 0.00066; n = 8). These data suggest that annexin A7 contributes to the shape of red blood cells and the osmotic resistance.
T9617 3075-3082 JJ denotes Osmotic
T9618 3083-3093 NN denotes resistance
T9619 3094-3100 NNS denotes curves
T9620 3101-3103 IN denotes of
T9621 3104-3107 JJ denotes red
T9623 3108-3113 NN denotes blood
T9622 3114-3119 NNS denotes cells
T9624 3120-3124 IN denotes from
T9625 3125-3129 JJ denotes wild
T9626 3130-3134 NN denotes type
T9628 3135-3138 CC denotes and
T9629 3139-3144 NN denotes anxA7
T9630 3144-3145 SYM denotes -
T9631 3145-3146 HYPH denotes /
T9632 3146-3147 SYM denotes -
T9633 3148-3154 JJ denotes mutant
T9627 3155-3159 NNS denotes mice
T9634 3159-3160 . denotes .
T9635 3160-3303 sentence denotes The osmotic resistance towards changes in the extracellular ionic strength is a convenient assay for analysis of the red blood cell integrity.
T9636 3161-3164 DT denotes The
T9638 3165-3172 JJ denotes osmotic
T9637 3173-3183 NN denotes resistance
T9640 3184-3191 IN denotes towards
T9641 3192-3199 NNS denotes changes
T9642 3200-3202 IN denotes in
T9643 3203-3206 DT denotes the
T9645 3207-3220 JJ denotes extracellular
T9646 3221-3226 JJ denotes ionic
T9644 3227-3235 NN denotes strength
T9639 3236-3238 VBZ denotes is
T9647 3239-3240 DT denotes a
T9649 3241-3251 JJ denotes convenient
T9648 3252-3257 NN denotes assay
T9650 3258-3261 IN denotes for
T9651 3262-3270 NN denotes analysis
T9652 3271-3273 IN denotes of
T9653 3274-3277 DT denotes the
T9655 3278-3281 JJ denotes red
T9657 3282-3287 NN denotes blood
T9656 3288-3292 NN denotes cell
T9654 3293-3302 NN denotes integrity
T9658 3302-3303 . denotes .
T9659 3303-3470 sentence denotes It is measured as a sodium chloride concentration in which cellular lysis starts (minimal resistance) up to a complete lysis (maximal resistance) of a red blood cell.
T9660 3304-3306 PRP denotes It
T9662 3307-3309 VBZ denotes is
T9661 3310-3318 VBN denotes measured
T9663 3319-3321 IN denotes as
T9664 3322-3323 DT denotes a
T9666 3324-3330 NN denotes sodium
T9667 3331-3339 NN denotes chloride
T9665 3340-3353 NN denotes concentration
T9668 3354-3356 IN denotes in
T9670 3357-3362 WDT denotes which
T9671 3363-3371 JJ denotes cellular
T9672 3372-3377 NN denotes lysis
T9669 3378-3384 VBZ denotes starts
T9673 3385-3386 -LRB- denotes (
T9675 3386-3393 JJ denotes minimal
T9674 3394-3404 NN denotes resistance
T9676 3404-3405 -RRB- denotes )
T9677 3406-3408 IN denotes up
T9678 3409-3411 IN denotes to
T9679 3412-3413 DT denotes a
T9681 3414-3422 JJ denotes complete
T9680 3423-3428 NN denotes lysis
T9682 3429-3430 -LRB- denotes (
T9684 3430-3437 JJ denotes maximal
T9683 3438-3448 NN denotes resistance
T9685 3448-3449 -RRB- denotes )
T9686 3450-3452 IN denotes of
T9687 3453-3454 DT denotes a
T9689 3455-3458 JJ denotes red
T9690 3459-3464 NN denotes blood
T9688 3465-3469 NN denotes cell
T9691 3469-3470 . denotes .
T9692 3470-3615 sentence denotes The osmotic resistance of annexin A7 deficient red blood cells is significantly increased compared to the one of wild type (p = 0.00066; n = 8).
T9693 3471-3474 DT denotes The
T9695 3475-3482 JJ denotes osmotic
T9694 3483-3493 NN denotes resistance
T9697 3494-3496 IN denotes of
T9698 3497-3504 NN denotes annexin
T9699 3505-3507 NN denotes A7
T9700 3508-3517 JJ denotes deficient
T9702 3518-3521 JJ denotes red
T9703 3522-3527 NN denotes blood
T9701 3528-3533 NNS denotes cells
T9696 3534-3536 VBZ denotes is
T9704 3537-3550 RB denotes significantly
T9705 3551-3560 JJ denotes increased
T9706 3561-3569 VBN denotes compared
T9707 3570-3572 IN denotes to
T9708 3573-3576 DT denotes the
T9709 3577-3580 CD denotes one
T9710 3581-3583 IN denotes of
T9711 3584-3588 JJ denotes wild
T9712 3589-3593 NN denotes type
T9713 3594-3595 -LRB- denotes (
T9715 3595-3596 NN denotes p
T9717 3597-3598 SYM denotes =
T9716 3599-3606 CD denotes 0.00066
T9718 3606-3607 : denotes ;
T9719 3608-3609 NN denotes n
T9720 3610-3611 SYM denotes =
T9714 3612-3613 CD denotes 8
T9721 3613-3614 -RRB- denotes )
T9722 3614-3615 . denotes .
T3819 3616-3621 DT denotes These
T3820 3622-3626 NNS denotes data
T3821 3627-3634 VBP denotes suggest
T3822 3635-3639 IN denotes that
T3824 3640-3647 NN denotes annexin
T3825 3648-3650 NN denotes A7
T3823 3651-3662 VBZ denotes contributes
T3826 3663-3665 IN denotes to
T3827 3666-3669 DT denotes the
T3828 3670-3675 NN denotes shape
T3829 3676-3678 IN denotes of
T3830 3679-3682 JJ denotes red
T3832 3683-3688 NN denotes blood
T3831 3689-3694 NNS denotes cells
T3833 3695-3698 CC denotes and
T3834 3699-3702 DT denotes the
T3836 3703-3710 JJ denotes osmotic
T3835 3711-3721 NN denotes resistance
T3837 3721-3722 . denotes .
T3838 3722-3900 sentence denotes As we have not observed a binding of annexin A7 to F-actin (data not shown) it could do so either by alteration of the membrane rigidity and/or by affecting the ion homeostasis.
T3839 3723-3725 IN denotes As
T3841 3726-3728 PRP denotes we
T3842 3729-3733 VBP denotes have
T3843 3734-3737 RB denotes not
T3840 3738-3746 VBN denotes observed
T3845 3747-3748 DT denotes a
T3846 3749-3756 NN denotes binding
T3847 3757-3759 IN denotes of
T3848 3760-3767 NN denotes annexin
T3849 3768-3770 NN denotes A7
T3850 3771-3773 IN denotes to
T3851 3774-3775 NN denotes F
T3853 3775-3776 HYPH denotes -
T3852 3776-3781 NN denotes actin
T3854 3782-3783 -LRB- denotes (
T3856 3783-3787 NNS denotes data
T3857 3788-3791 RB denotes not
T3855 3792-3797 VBN denotes shown
T3858 3797-3798 -RRB- denotes )
T3859 3799-3801 PRP denotes it
T3860 3802-3807 MD denotes could
T3844 3808-3810 VB denotes do
T3861 3811-3813 RB denotes so
T3862 3814-3820 CC denotes either
T3863 3821-3823 IN denotes by
T3864 3824-3834 NN denotes alteration
T3865 3835-3837 IN denotes of
T3866 3838-3841 DT denotes the
T3868 3842-3850 NN denotes membrane
T3867 3851-3859 NN denotes rigidity
T3869 3860-3863 CC denotes and
T3870 3863-3864 HYPH denotes /
T3871 3864-3866 CC denotes or
T3872 3867-3869 IN denotes by
T3873 3870-3879 VBG denotes affecting
T3874 3880-3883 DT denotes the
T3876 3884-3887 NN denotes ion
T3875 3888-3899 NN denotes homeostasis
T3877 3899-3900 . denotes .
R1795 T3280 T3281 det The,lack
R1796 T3281 T3282 nsubj lack,changes
R1797 T3283 T3281 prep of,lack
R1798 T3284 T3285 compound annexin,A7
R1799 T3285 T3283 pobj A7,of
R1800 T3286 T3287 amod red,cell
R1801 T3287 T3282 dobj cell,changes
R1802 T3288 T3287 compound blood,cell
R1803 T3289 T3287 appos morphology,cell
R1804 T3290 T3289 cc and,morphology
R1805 T3291 T3292 amod osmotic,resistance
R1806 T3292 T3289 conj resistance,morphology
R1807 T3294 T3295 mark As,is
R1808 T3295 T3298 advcl is,studied
R1809 T3296 T3297 compound annexin,A7
R1810 T3297 T3295 nsubj A7,is
R1811 T3299 T3300 det a,component
R1812 T3300 T3295 attr component,is
R1813 T3301 T3300 prep of,component
R1814 T3302 T3303 amod red,cell
R1815 T3303 T3305 compound cell,vesicles
R1816 T3304 T3303 compound blood,cell
R1817 T3305 T3301 pobj vesicles,of
R1818 T3306 T3298 nsubj we,studied
R1819 T3307 T3308 det the,consequences
R1820 T3308 T3298 dobj consequences,studied
R1821 T3309 T3308 prep of,consequences
R1822 T3310 T3311 det the,loss
R1823 T3311 T3309 pobj loss,of
R1824 T3312 T3311 prep of,loss
R1825 T3313 T3314 det the,protein
R1826 T3314 T3312 pobj protein,of
R1827 T3315 T3298 prep in,studied
R1828 T3316 T3317 det the,mouse
R1829 T3317 T3315 pobj mouse,in
R1830 T3318 T3317 nmod anxA7,mouse
R1831 T3319 T3318 punct -,anxA7
R1832 T3320 T3318 punct /,anxA7
R1833 T3321 T3318 punct -,anxA7
R1834 T3322 T3298 prep for,studied
R1835 T3323 T3324 amod red,cell
R1836 T3324 T3322 pobj cell,for
R1837 T3325 T3324 compound blood,cell
R1838 T3326 T3324 appos morphology,cell
R1839 T3327 T3326 cc and,morphology
R1840 T3328 T3329 amod osmotic,resistance
R1841 T3329 T3326 conj resistance,morphology
R1842 T3330 T3298 punct .,studied
R1843 T3332 T3333 prep In,revealed
R1844 T3334 T3335 det a,smear
R1845 T3335 T3332 pobj smear,In
R1846 T3336 T3335 amod standard,smear
R1847 T3337 T3335 punct ',smear
R1848 T3338 T3335 compound blood,smear
R1849 T3339 T3333 punct ',revealed
R1850 T3340 T3341 nsubj we,note
R1851 T3341 T3333 ccomp note,revealed
R1852 T3342 T3341 aux did,note
R1853 T3343 T3341 neg not,note
R1854 T3344 T3345 det a,deformation
R1855 T3345 T3341 dobj deformation,note
R1856 T3346 T3345 prep of,deformation
R1857 T3347 T3348 det the,cells
R1858 T3348 T3346 pobj cells,of
R1859 T3349 T3333 punct ", ",revealed
R1860 T3350 T3333 advmod however,revealed
R1861 T3351 T3352 amod dark,field
R1862 T3352 T3353 compound field,microscopy
R1863 T3353 T3333 nsubj microscopy,revealed
R1864 T3354 T3333 dobj changes,revealed
R1865 T3355 T3354 prep in,changes
R1866 T3356 T3355 pobj shape,in
R1867 T3357 T3356 cc and,shape
R1868 T3358 T3356 conj diameter,shape
R1869 T3359 T3356 prep of,shape
R1870 T3360 T3361 det the,cells
R1871 T3361 T3359 pobj cells,of
R1872 T3362 T3361 nmod anxA7,cells
R1873 T3363 T3362 punct -,anxA7
R1874 T3364 T3362 punct /,anxA7
R1875 T3365 T3362 punct -,anxA7
R1876 T3366 T3361 amod red,cells
R1877 T3367 T3361 compound blood,cells
R1878 T3368 T3333 punct .,revealed
R1879 T3370 T3371 nsubj They,had
R1880 T3372 T3373 det a,diameter
R1881 T3373 T3371 dobj diameter,had
R1882 T3374 T3375 advmod statistically,significant
R1883 T3375 T3373 amod significant,diameter
R1884 T3376 T3373 amod larger,diameter
R1885 T3377 T3373 compound cell,diameter
R1886 T3378 T3373 prep of,diameter
R1887 T3379 T3380 nummod 6.0,μm
R1888 T3380 T3378 pobj μm,of
R1889 T3381 T3373 prep compared,diameter
R1890 T3382 T3381 prep to,compared
R1891 T3383 T3384 amod wild,type
R1892 T3384 T3382 pobj type,to
R1893 T3385 T3384 prep with,type
R1894 T3386 T3387 nummod 5.7,μm
R1895 T3387 T3385 pobj μm,with
R1896 T3388 T3389 punct (,40
R1897 T3389 T3373 parataxis 40,diameter
R1898 T3390 T3391 nsubj p,0.015
R1899 T3391 T3389 ccomp 0.015,40
R1900 T3392 T3391 punct =,0.015
R1901 T3393 T3389 punct ", ",40
R1902 T3394 T3389 nsubj n,40
R1903 T3395 T3389 punct =,40
R1904 T3396 T3389 punct ),40
R1905 T3397 T3373 punct ", ",diameter
R1906 T3398 T3399 det a,impression
R1907 T3399 T3373 conj impression,diameter
R1908 T3400 T3401 advmod remarkably,lower
R1909 T3401 T3399 amod lower,impression
R1910 T3402 T3399 amod emphasized,impression
R1911 T3403 T3399 amod central,impression
R1912 T3404 T3399 cc and,impression
R1913 T3405 T3406 det a,shape
R1914 T3406 T3399 conj shape,impression
R1915 T3407 T3408 advmod more,flat
R1916 T3408 T3406 amod flat,shape
R1917 T3409 T3410 punct (,Fig.
R1918 T3410 T3406 parataxis Fig.,shape
R1919 T3411 T3410 nummod 4,Fig.
R1920 T3412 T3410 punct ),Fig.
R1921 T3413 T3371 punct .,had
R1922 T3415 T3416 det No,change
R1923 T3416 T3418 nsubjpass change,measured
R1924 T3417 T3416 amod significant,change
R1925 T3419 T3416 prep in,change
R1926 T3420 T3421 det the,volume
R1927 T3421 T3419 pobj volume,in
R1928 T3422 T3421 amod mean,volume
R1929 T3423 T3421 amod corpuscular,volume
R1930 T3424 T3421 punct (,volume
R1931 T3425 T3421 appos MCV,volume
R1932 T3426 T3418 punct ),measured
R1933 T3427 T3418 auxpass was,measured
R1934 T3428 T3418 prep with,measured
R1935 T3429 T3430 det the,counter
R1936 T3430 T3428 pobj counter,with
R1937 T3431 T3430 nmod ADVIA,counter
R1938 T3432 T3431 nummod 120,ADVIA
R1939 T3433 T3430 compound cell,counter
R1940 T3434 T3418 punct .,measured
R1941 T3437 T3438 nsubj Shape,influence
R1942 T3439 T3437 cc and,Shape
R1943 T3440 T3437 conj MCV,Shape
R1944 T3441 T3437 prep of,Shape
R1945 T3442 T3443 det a,cell
R1946 T3443 T3441 pobj cell,of
R1947 T3444 T3443 amod red,cell
R1948 T3445 T3443 compound blood,cell
R1949 T3446 T3438 advmod essentially,influence
R1950 T3447 T3448 poss its,function
R1951 T3448 T3438 dobj function,influence
R1952 T3449 T3448 cc and,function
R1953 T3450 T3451 amod capillary,passage
R1954 T3451 T3448 conj passage,function
R1955 T3452 T3438 punct .,influence
R1956 T3454 T3455 det The,form
R1957 T3455 T3458 nsubj form,depends
R1958 T3456 T3455 amod typical,form
R1959 T3457 T3455 compound biconcave,form
R1960 T3459 T3458 prep on,depends
R1961 T3460 T3461 amod various,influences
R1962 T3461 T3459 pobj influences,on
R1963 T3462 T3461 prep including,influences
R1964 T3463 T3464 det the,composition
R1965 T3464 T3462 pobj composition,including
R1966 T3465 T3466 compound membrane,lipid
R1967 T3466 T3464 compound lipid,composition
R1968 T3467 T3464 cc and,composition
R1969 T3468 T3469 det the,cytoskeleton
R1970 T3469 T3464 conj cytoskeleton,composition
R1971 T3470 T3469 amod submembranous,cytoskeleton
R1972 T3471 T3472 punct [,32
R1973 T3472 T3469 parataxis 32,cytoskeleton
R1974 T3473 T3472 punct ],32
R1975 T3474 T3475 advmod where,play
R1976 T3475 T3469 relcl play,cytoskeleton
R1977 T3476 T3477 compound annexin,A7
R1978 T3477 T3475 nsubj A7,play
R1979 T3478 T3475 aux might,play
R1980 T3479 T3480 det a,role
R1981 T3480 T3475 dobj role,play
R1982 T3481 T3458 punct .,depends
R1983 T3483 T3484 det The,resistance
R1984 T3484 T3486 nsubj resistance,is
R1985 T3485 T3484 amod osmotic,resistance
R1986 T3487 T3484 punct ", ",resistance
R1987 T3488 T3489 dep which,is
R1988 T3489 T3484 relcl is,resistance
R1989 T3490 T3491 det the,resistance
R1990 T3491 T3489 attr resistance,is
R1991 T3492 T3491 prep towards,resistance
R1992 T3493 T3492 pobj changes,towards
R1993 T3494 T3489 prep in,is
R1994 T3495 T3496 det the,strength
R1995 T3496 T3494 pobj strength,in
R1996 T3497 T3496 amod extracellular,strength
R1997 T3498 T3496 amod ionic,strength
R1998 T3499 T3486 punct ", ",is
R1999 T3500 T3501 det a,assay
R2000 T3501 T3486 attr assay,is
R2001 T3502 T3501 amod convenient,assay
R2002 T3503 T3501 prep for,assay
R2003 T3504 T3503 pobj analysis,for
R2004 T3505 T3504 prep of,analysis
R2005 T3506 T3507 det the,integrity
R2006 T3507 T3505 pobj integrity,of
R2007 T3508 T3509 amod red,cell
R2008 T3509 T3507 compound cell,integrity
R2009 T3510 T3509 compound blood,cell
R2010 T3511 T3486 punct .,is
R2011 T3513 T3514 nsubjpass It,measured
R2012 T3515 T3514 auxpass is,measured
R2013 T3516 T3514 prep as,measured
R2014 T3517 T3518 det the,concentration
R2015 T3518 T3516 pobj concentration,as
R2016 T3519 T3520 compound sodium,chloride
R2017 T3520 T3518 compound chloride,concentration
R2018 T3521 T3522 prep at,starts
R2019 T3522 T3518 relcl starts,concentration
R2020 T3523 T3521 pobj which,at
R2021 T3524 T3525 amod cellular,lysis
R2022 T3525 T3522 nsubj lysis,starts
R2023 T3526 T3518 punct (,concentration
R2024 T3527 T3528 amod minimal,resistance
R2025 T3528 T3518 appos resistance,concentration
R2026 T3529 T3518 punct ),concentration
R2027 T3530 T3518 prep up,concentration
R2028 T3531 T3530 prep to,up
R2029 T3532 T3533 det a,lysis
R2030 T3533 T3531 pobj lysis,to
R2031 T3534 T3533 amod complete,lysis
R2032 T3535 T3533 punct (,lysis
R2033 T3536 T3537 amod maximal,resistance
R2034 T3537 T3533 appos resistance,lysis
R2035 T3538 T3533 punct ),lysis
R2036 T3539 T3533 prep of,lysis
R2037 T3540 T3541 det the,cells
R2038 T3541 T3539 pobj cells,of
R2039 T3542 T3543 punct [,33
R2040 T3543 T3514 parataxis 33,measured
R2041 T3544 T3543 punct ],33
R2042 T3545 T3514 punct .,measured
R2043 T3547 T3548 amod Osmotic,resistance
R2044 T3548 T3549 nsubj resistance,increases
R2045 T3550 T3549 prep with,increases
R2046 T3551 T3552 amod higher,MCV
R2047 T3552 T3550 pobj MCV,with
R2048 T3553 T3552 punct ", ",MCV
R2049 T3554 T3555 amod larger,area
R2050 T3555 T3552 conj area,MCV
R2051 T3556 T3555 compound surface,area
R2052 T3557 T3555 cc and,area
R2053 T3558 T3559 det a,degree
R2054 T3559 T3555 conj degree,area
R2055 T3560 T3559 amod higher,degree
R2056 T3561 T3559 prep of,degree
R2057 T3562 T3563 amod cellular,metabolism
R2058 T3563 T3561 pobj metabolism,of
R2059 T3564 T3552 acl stabilizing,MCV
R2060 T3565 T3566 det the,levels
R2061 T3566 T3564 dobj levels,stabilizing
R2062 T3567 T3566 amod intracellular,levels
R2063 T3568 T3566 compound ion,levels
R2064 T3569 T3549 punct .,increases
R2065 T3571 T3572 amod Aged,cells
R2066 T3572 T3575 nsubj cells,show
R2067 T3573 T3572 amod red,cells
R2068 T3574 T3572 compound blood,cells
R2069 T3576 T3577 det a,MCV
R2070 T3577 T3575 dobj MCV,show
R2071 T3578 T3577 amod lowered,MCV
R2072 T3579 T3577 cc and,MCV
R2073 T3580 T3581 amod lower,resistance
R2074 T3581 T3577 conj resistance,MCV
R2075 T3582 T3581 amod osmotic,resistance
R2076 T3583 T3584 punct [,33
R2077 T3584 T3575 parataxis 33,show
R2078 T3585 T3584 punct ],33
R2079 T3586 T3575 punct .,show
R2080 T3588 T3589 amod Similar,properties
R2081 T3589 T3590 nsubjpass properties,observed
R2082 T3591 T3590 aux can,observed
R2083 T3592 T3590 auxpass be,observed
R2084 T3593 T3590 prep in,observed
R2085 T3594 T3595 amod red,cells
R2086 T3595 T3593 pobj cells,in
R2087 T3596 T3595 compound blood,cells
R2088 T3597 T3595 prep with,cells
R2089 T3598 T3599 amod lowered,permeability
R2090 T3599 T3597 pobj permeability,with
R2091 T3600 T3599 compound membrane,permeability
R2092 T3601 T3590 punct .,observed
R2093 T3603 T3604 det The,resistance
R2094 T3604 T3606 nsubj resistance,is
R2095 T3605 T3604 amod osmotic,resistance
R2096 T3607 T3604 prep of,resistance
R2097 T3608 T3609 compound annexin,A7
R2098 T3609 T3610 npadvmod A7,deficient
R2099 T3610 T3611 amod deficient,cells
R2100 T3611 T3607 pobj cells,of
R2101 T3612 T3611 amod red,cells
R2102 T3613 T3611 compound blood,cells
R2103 T3614 T3615 advmod significantly,increased
R2104 T3615 T3606 acomp increased,is
R2105 T3616 T3606 prep compared,is
R2106 T3617 T3616 prep to,compared
R2107 T3618 T3619 amod wild,type
R2108 T3619 T3620 compound type,cells
R2109 T3620 T3617 pobj cells,to
R2110 T3621 T3606 punct .,is
R2111 T3623 T3624 det The,value
R2112 T3624 T3627 nsubjpass value,observed
R2113 T3625 T3626 amod minimal,resistance
R2114 T3626 T3624 compound resistance,value
R2115 T3628 T3624 prep of,value
R2116 T3629 T3630 compound knock,out
R2117 T3630 T3631 compound out,cells
R2118 T3631 T3628 pobj cells,of
R2119 T3632 T3627 auxpass is,observed
R2120 T3633 T3627 prep in,observed
R2121 T3634 T3635 det a,solution
R2122 T3635 T3633 pobj solution,in
R2123 T3636 T3637 nummod 0.60,%
R2124 T3637 T3635 compound %,solution
R2125 T3638 T3635 compound NaCl,solution
R2126 T3639 T3635 cc versus,solution
R2127 T3640 T3641 nummod 0.65,%
R2128 T3641 T3635 conj %,solution
R2129 T3642 T3641 prep for,%
R2130 T3643 T3644 amod wild,type
R2131 T3644 T3645 compound type,cells
R2132 T3645 T3642 pobj cells,for
R2133 T3646 T3627 punct .,observed
R2134 T3648 T3649 nummod 50,%
R2135 T3649 T3650 compound %,haemolysis
R2136 T3650 T3651 nsubjpass haemolysis,achieved
R2137 T3652 T3651 auxpass is,achieved
R2138 T3653 T3651 prep at,achieved
R2139 T3654 T3655 nummod 0.516,%
R2140 T3655 T3656 compound %,solution
R2141 T3656 T3653 pobj solution,at
R2142 T3657 T3656 compound NaCl,solution
R2143 T3658 T3656 prep for,solution
R2144 T3659 T3660 compound knock,out
R2145 T3660 T3658 pobj out,for
R2146 T3661 T3656 cc versus,solution
R2147 T3662 T3663 nummod 0.564,%
R2148 T3663 T3664 compound %,NaCl
R2149 T3664 T3656 conj NaCl,solution
R2150 T3665 T3664 prep for,NaCl
R2151 T3666 T3667 amod wild,type
R2152 T3667 T3668 compound type,cells
R2153 T3668 T3665 pobj cells,for
R2154 T3669 T3670 punct (,Fig.
R2155 T3670 T3651 parataxis Fig.,achieved
R2156 T3671 T3672 compound mean,values
R2157 T3672 T3670 dep values,Fig.
R2158 T3673 T3670 punct ", ",Fig.
R2159 T3674 T3675 nsubj p,0.00066
R2160 T3675 T3670 ccomp 0.00066,Fig.
R2161 T3676 T3675 punct =,0.00066
R2162 T3677 T3670 punct ", ",Fig.
R2163 T3678 T3679 nsubj n,8
R2164 T3679 T3670 ccomp 8,Fig.
R2165 T3680 T3679 punct =,8
R2166 T3681 T3670 punct ;,Fig.
R2167 T3682 T3670 nummod 5,Fig.
R2168 T3683 T3670 punct ),Fig.
R2169 T3684 T3651 punct .,achieved
R2170 T3686 T3687 det The,width
R2171 T3687 T3689 nsubj width,is
R2172 T3688 T3687 compound resistance,width
R2173 T3690 T3687 prep of,width
R2174 T3691 T3692 det the,cells
R2175 T3692 T3690 pobj cells,of
R2176 T3693 T3694 compound knock,out
R2177 T3694 T3692 compound out,cells
R2178 T3695 T3696 advmod slightly,lower
R2179 T3696 T3689 acomp lower,is
R2180 T3697 T3698 punct (,solution
R2181 T3698 T3696 parataxis solution,lower
R2182 T3699 T3700 nummod 0.20,%
R2183 T3700 T3701 nmod %,NaCl
R2184 T3701 T3698 compound NaCl,solution
R2185 T3702 T3703 advmod instead,of
R2186 T3703 T3700 cc of,%
R2187 T3704 T3705 nummod 0.25,%
R2188 T3705 T3700 conj %,%
R2189 T3706 T3698 punct ),solution
R2190 T3707 T3689 punct .,is
R2191 T3709 T3710 nsubj We,performed
R2192 T3711 T3710 advmod also,performed
R2193 T3712 T3713 amod direct,measurements
R2194 T3713 T3710 dobj measurements,performed
R2195 T3714 T3713 prep of,measurements
R2196 T3715 T3716 det the,deformability
R2197 T3716 T3714 pobj deformability,of
R2198 T3717 T3718 amod red,cell
R2199 T3718 T3716 compound cell,deformability
R2200 T3719 T3718 compound blood,cell
R2201 T3720 T3716 compound membrane,deformability
R2202 T3721 T3710 punct ", ",performed
R2203 T3722 T3723 aux to,characterize
R2204 T3723 T3710 advcl characterize,performed
R2205 T3724 T3723 advmod further,characterize
R2206 T3725 T3726 det a,role
R2207 T3726 T3723 dobj role,characterize
R2208 T3727 T3726 amod possible,role
R2209 T3728 T3726 prep of,role
R2210 T3729 T3730 compound annexin,A7
R2211 T3730 T3728 pobj A7,of
R2212 T3731 T3726 prep on,role
R2213 T3732 T3733 det the,stability
R2214 T3733 T3731 pobj stability,on
R2215 T3734 T3733 compound membrane,stability
R2216 T3735 T3710 punct .,performed
R2217 T3737 T3738 prep With,tried
R2218 T3739 T3740 compound micropipette,experiments
R2219 T3740 T3737 pobj experiments,With
R2220 T3741 T3738 nsubj we,tried
R2221 T3742 T3743 aux to,correlate
R2222 T3743 T3738 xcomp correlate,tried
R2223 T3744 T3745 amod mechanical,characteristics
R2224 T3745 T3743 dobj characteristics,correlate
R2225 T3746 T3745 prep of,characteristics
R2226 T3747 T3748 det the,cells
R2227 T3748 T3746 pobj cells,of
R2228 T3749 T3748 amod red,cells
R2229 T3750 T3748 compound blood,cells
R2230 T3751 T3743 prep with,correlate
R2231 T3752 T3753 poss their,morphology
R2232 T3753 T3751 pobj morphology,with
R2233 T3754 T3738 punct .,tried
R2234 T3756 T3757 nsubj We,observe
R2235 T3758 T3757 aux could,observe
R2236 T3759 T3757 neg not,observe
R2237 T3760 T3761 det any,difference
R2238 T3761 T3757 dobj difference,observe
R2239 T3762 T3763 advmod statistically,significant
R2240 T3763 T3761 amod significant,difference
R2241 T3764 T3761 prep of,difference
R2242 T3765 T3764 pobj values,of
R2243 T3766 T3765 acl describing,values
R2244 T3767 T3768 compound membrane,rigidity
R2245 T3768 T3766 dobj rigidity,describing
R2246 T3769 T3768 cc and,rigidity
R2247 T3770 T3771 compound lysis,force
R2248 T3771 T3768 conj force,rigidity
R2249 T3772 T3757 prep in,observe
R2250 T3773 T3774 det these,experiments
R2251 T3774 T3772 pobj experiments,in
R2252 T3775 T3776 punct (,M.
R2253 T3776 T3757 meta M.,observe
R2254 T3777 T3776 nmod Heil,M.
R2255 T3778 T3776 punct ", ",M.
R2256 T3779 T3776 conj B.,M.
R2257 T3780 T3779 nmod Hoffmann,B.
R2258 T3781 T3779 cc and,B.
R2259 T3782 T3779 conj R.,B.
R2260 T3783 T3782 nmod Merkel,R.
R2261 T3784 T3782 punct ", ",R.
R2262 T3785 T3786 amod unpublished,results
R2263 T3786 T3782 conj results,R.
R2264 T3787 T3786 punct ),results
R2265 T3788 T3757 punct .,observe
R2266 T3790 T3791 advmod However,was
R2267 T3792 T3791 punct ", ",was
R2268 T3793 T3794 det the,distinction
R2269 T3794 T3791 nsubj distinction,was
R2270 T3795 T3794 acl observed,distinction
R2271 T3796 T3795 prep in,observed
R2272 T3797 T3798 det the,experiments
R2273 T3798 T3796 pobj experiments,in
R2274 T3799 T3800 amod osmotic,resistance
R2275 T3800 T3798 compound resistance,experiments
R2276 T3801 T3802 advmod highly,significant
R2277 T3802 T3791 acomp significant,was
R2278 T3803 T3791 cc and,was
R2279 T3804 T3791 conj reflects,was
R2280 T3805 T3806 det a,value
R2281 T3806 T3804 dobj value,reflects
R2282 T3807 T3806 amod mean,value
R2283 T3808 T3806 prep over,value
R2284 T3809 T3810 det a,number
R2285 T3810 T3808 pobj number,over
R2286 T3811 T3810 amod high,number
R2287 T3812 T3810 prep of,number
R2288 T3813 T3814 amod different,cells
R2289 T3814 T3812 pobj cells,of
R2290 T3815 T3814 amod red,cells
R2291 T3816 T3814 compound blood,cells
R2292 T3817 T3791 punct .,was
R2293 T3819 T3820 det These,data
R2294 T3820 T3821 nsubj data,suggest
R2295 T3822 T3823 mark that,contributes
R2296 T3823 T3821 ccomp contributes,suggest
R2297 T3824 T3825 compound annexin,A7
R2298 T3825 T3823 nsubj A7,contributes
R2299 T3826 T3823 prep to,contributes
R2300 T3827 T3828 det the,shape
R2301 T3828 T3826 pobj shape,to
R2302 T3829 T3828 prep of,shape
R2303 T3830 T3831 amod red,cells
R2304 T3831 T3829 pobj cells,of
R2305 T3832 T3831 compound blood,cells
R2306 T3833 T3828 cc and,shape
R2307 T3834 T3835 det the,resistance
R2308 T3835 T3828 conj resistance,shape
R2309 T3836 T3835 amod osmotic,resistance
R2310 T3837 T3821 punct .,suggest
R2311 T3839 T3840 mark As,observed
R2312 T3840 T3844 advcl observed,do
R2313 T3841 T3840 nsubj we,observed
R2314 T3842 T3840 aux have,observed
R2315 T3843 T3840 neg not,observed
R2316 T3845 T3846 det a,binding
R2317 T3846 T3840 dobj binding,observed
R2318 T3847 T3846 prep of,binding
R2319 T3848 T3849 compound annexin,A7
R2320 T3849 T3847 pobj A7,of
R2321 T3850 T3846 prep to,binding
R2322 T3851 T3852 compound F,actin
R2323 T3852 T3850 pobj actin,to
R2324 T3853 T3852 punct -,actin
R2325 T3854 T3855 punct (,shown
R2326 T3855 T3840 parataxis shown,observed
R2327 T3856 T3855 nsubj data,shown
R2328 T3857 T3855 neg not,shown
R2329 T3858 T3855 punct ),shown
R2330 T3859 T3844 nsubj it,do
R2331 T3860 T3844 aux could,do
R2332 T3861 T3844 advmod so,do
R2333 T3862 T3863 preconj either,by
R2334 T3863 T3844 prep by,do
R2335 T3864 T3863 pobj alteration,by
R2336 T3865 T3864 prep of,alteration
R2337 T3866 T3867 det the,rigidity
R2338 T3867 T3865 pobj rigidity,of
R2339 T3868 T3867 compound membrane,rigidity
R2340 T3869 T3863 cc and,by
R2341 T3870 T3869 punct /,and
R2342 T3871 T3869 cc or,and
R2343 T3872 T3863 conj by,by
R2344 T3873 T3872 pcomp affecting,by
R2345 T3874 T3875 det the,homeostasis
R2346 T3875 T3873 dobj homeostasis,affecting
R2347 T3876 T3875 compound ion,homeostasis
R2348 T3877 T3844 punct .,do
R5956 T9489 T9490 amod Dark,field
R5957 T9490 T9491 compound field,microscopy
R5958 T9492 T9491 prep of,microscopy
R5959 T9493 T9494 amod red,cells
R5960 T9494 T9492 pobj cells,of
R5961 T9495 T9494 compound blood,cells
R5962 T9496 T9494 prep from,cells
R5963 T9497 T9498 nmod anxA7,mutant
R5964 T9498 T9502 nmod mutant,mice
R5965 T9499 T9497 punct -,anxA7
R5966 T9500 T9497 punct /,anxA7
R5967 T9501 T9497 punct -,anxA7
R5968 T9502 T9496 pobj mice,from
R5969 T9503 T9504 punct (,B
R5970 T9504 T9498 parataxis B,mutant
R5971 T9505 T9504 punct ),B
R5972 T9506 T9498 cc and,mutant
R5973 T9507 T9508 amod wild,type
R5974 T9508 T9498 conj type,mutant
R5975 T9509 T9510 punct (,A
R5976 T9510 T9502 parataxis A,mice
R5977 T9511 T9510 punct ),A
R5978 T9512 T9491 punct .,microscopy
R5979 T9514 T9515 det The,diameters
R5980 T9515 T9518 nsubj diameters,are
R5981 T9516 T9515 amod mean,diameters
R5982 T9517 T9515 amod cellular,diameters
R5983 T9519 T9520 nummod 6.0,μm
R5984 T9520 T9518 attr μm,are
R5985 T9521 T9520 cc and,μm
R5986 T9522 T9523 nummod 5.7,μm
R5987 T9523 T9520 conj μm,μm
R5988 T9524 T9518 punct ", ",are
R5989 T9525 T9518 advmod respectively,are
R5990 T9526 T9518 punct .,are
R5991 T9528 T9529 det The,differences
R5992 T9529 T9531 nsubj differences,are
R5993 T9530 T9529 compound size,differences
R5994 T9532 T9533 advmod statistically,significant
R5995 T9533 T9531 acomp significant,are
R5996 T9534 T9535 punct (,40
R5997 T9535 T9531 parataxis 40,are
R5998 T9536 T9537 nsubj p,0.015
R5999 T9537 T9535 ccomp 0.015,40
R6000 T9538 T9537 punct =,0.015
R6001 T9539 T9535 punct ;,40
R6002 T9540 T9535 nsubj n,40
R6003 T9541 T9535 punct =,40
R6004 T9542 T9535 punct ),40
R6005 T9543 T9531 punct .,are
R6006 T9545 T9546 dep Bar,μm
R6007 T9547 T9546 punct ", ",μm
R6008 T9548 T9546 nummod 3,μm
R6009 T9549 T9546 punct .,μm
R6014 T9617 T9618 amod Osmotic,resistance
R6015 T9618 T9619 compound resistance,curves
R6016 T9620 T9619 prep of,curves
R6017 T9621 T9622 amod red,cells
R6018 T9622 T9620 pobj cells,of
R6019 T9623 T9622 compound blood,cells
R6020 T9624 T9619 prep from,curves
R6021 T9625 T9626 amod wild,type
R6022 T9626 T9627 nmod type,mice
R6023 T9627 T9624 pobj mice,from
R6024 T9628 T9626 cc and,type
R6025 T9629 T9626 conj anxA7,type
R6026 T9630 T9629 punct -,anxA7
R6027 T9631 T9629 punct /,anxA7
R6028 T9632 T9629 punct -,anxA7
R6029 T9633 T9627 amod mutant,mice
R6030 T9634 T9619 punct .,curves
R6031 T9636 T9637 det The,resistance
R6032 T9637 T9639 nsubj resistance,is
R6033 T9638 T9637 amod osmotic,resistance
R6034 T9640 T9637 prep towards,resistance
R6035 T9641 T9640 pobj changes,towards
R6036 T9642 T9641 prep in,changes
R6037 T9643 T9644 det the,strength
R6038 T9644 T9642 pobj strength,in
R6039 T9645 T9644 amod extracellular,strength
R6040 T9646 T9644 amod ionic,strength
R6041 T9647 T9648 det a,assay
R6042 T9648 T9639 attr assay,is
R6043 T9649 T9648 amod convenient,assay
R6044 T9650 T9648 prep for,assay
R6045 T9651 T9650 pobj analysis,for
R6046 T9652 T9651 prep of,analysis
R6047 T9653 T9654 det the,integrity
R6048 T9654 T9652 pobj integrity,of
R6049 T9655 T9656 amod red,cell
R6050 T9656 T9654 compound cell,integrity
R6051 T9657 T9656 compound blood,cell
R6052 T9658 T9639 punct .,is
R6053 T9660 T9661 nsubjpass It,measured
R6054 T9662 T9661 auxpass is,measured
R6055 T9663 T9661 prep as,measured
R6056 T9664 T9665 det a,concentration
R6057 T9665 T9663 pobj concentration,as
R6058 T9666 T9667 compound sodium,chloride
R6059 T9667 T9665 compound chloride,concentration
R6060 T9668 T9669 prep in,starts
R6061 T9669 T9665 relcl starts,concentration
R6062 T9670 T9668 pobj which,in
R6063 T9671 T9672 amod cellular,lysis
R6064 T9672 T9669 nsubj lysis,starts
R6065 T9673 T9674 punct (,resistance
R6066 T9674 T9665 parataxis resistance,concentration
R6067 T9675 T9674 amod minimal,resistance
R6068 T9676 T9674 punct ),resistance
R6069 T9677 T9665 prep up,concentration
R6070 T9678 T9677 prep to,up
R6071 T9679 T9680 det a,lysis
R6072 T9680 T9678 pobj lysis,to
R6073 T9681 T9680 amod complete,lysis
R6074 T9682 T9683 punct (,resistance
R6075 T9683 T9680 parataxis resistance,lysis
R6076 T9684 T9683 amod maximal,resistance
R6077 T9685 T9683 punct ),resistance
R6078 T9686 T9680 prep of,lysis
R6079 T9687 T9688 det a,cell
R6080 T9688 T9686 pobj cell,of
R6081 T9689 T9688 amod red,cell
R6082 T9690 T9688 compound blood,cell
R6083 T9691 T9661 punct .,measured
R6084 T9693 T9694 det The,resistance
R6085 T9694 T9696 nsubj resistance,is
R6086 T9695 T9694 amod osmotic,resistance
R6087 T9697 T9694 prep of,resistance
R6088 T9698 T9699 compound annexin,A7
R6089 T9699 T9700 npadvmod A7,deficient
R6090 T9700 T9701 amod deficient,cells
R6091 T9701 T9697 pobj cells,of
R6092 T9702 T9701 amod red,cells
R6093 T9703 T9701 compound blood,cells
R6094 T9704 T9705 advmod significantly,increased
R6095 T9705 T9696 acomp increased,is
R6096 T9706 T9696 prep compared,is
R6097 T9707 T9706 prep to,compared
R6098 T9708 T9709 det the,one
R6099 T9709 T9707 pobj one,to
R6100 T9710 T9709 prep of,one
R6101 T9711 T9712 amod wild,type
R6102 T9712 T9710 pobj type,of
R6103 T9713 T9714 punct (,8
R6104 T9714 T9696 parataxis 8,is
R6105 T9715 T9716 nsubj p,0.00066
R6106 T9716 T9714 ccomp 0.00066,8
R6107 T9717 T9716 punct =,0.00066
R6108 T9718 T9714 punct ;,8
R6109 T9719 T9714 nsubj n,8
R6110 T9720 T9714 punct =,8
R6111 T9721 T9714 punct ),8
R6112 T9722 T9696 punct .,is