Id |
Subject |
Object |
Predicate |
Lexical cue |
T1606 |
0-3 |
NN |
denotes |
RNA |
T1607 |
4-12 |
NN |
denotes |
Analysis |
T1608 |
12-208 |
sentence |
denotes |
RT-PCR amplification from exon 7 to 11 from total heart RNA amplified the expected 600-base pair (bp) fragment in MCAD+/+ and MCAD+/− mice, and a 1.5-kb fragment in MCAD−/− mice (data not shown). |
T1609 |
13-15 |
NN |
denotes |
RT |
T1611 |
15-16 |
HYPH |
denotes |
- |
T1610 |
16-19 |
NN |
denotes |
PCR |
T1612 |
20-33 |
NN |
denotes |
amplification |
T1614 |
34-38 |
IN |
denotes |
from |
T1615 |
39-43 |
NN |
denotes |
exon |
T1616 |
44-45 |
CD |
denotes |
7 |
T1617 |
46-48 |
IN |
denotes |
to |
T1618 |
49-51 |
CD |
denotes |
11 |
T1619 |
52-56 |
IN |
denotes |
from |
T1620 |
57-62 |
JJ |
denotes |
total |
T1622 |
63-68 |
NN |
denotes |
heart |
T1621 |
69-72 |
NN |
denotes |
RNA |
T1613 |
73-82 |
VBD |
denotes |
amplified |
T1623 |
83-86 |
DT |
denotes |
the |
T1625 |
87-95 |
VBN |
denotes |
expected |
T1626 |
96-99 |
CD |
denotes |
600 |
T1628 |
99-100 |
HYPH |
denotes |
- |
T1629 |
100-104 |
NN |
denotes |
base |
T1627 |
105-109 |
NN |
denotes |
pair |
T1630 |
110-111 |
-LRB- |
denotes |
( |
T1631 |
111-113 |
NN |
denotes |
bp |
T1632 |
113-114 |
-RRB- |
denotes |
) |
T1624 |
115-123 |
NN |
denotes |
fragment |
T1633 |
124-126 |
IN |
denotes |
in |
T1634 |
127-131 |
NN |
denotes |
MCAD |
T1636 |
131-132 |
SYM |
denotes |
+ |
T1637 |
132-133 |
HYPH |
denotes |
/ |
T1638 |
133-134 |
SYM |
denotes |
+ |
T1639 |
135-138 |
CC |
denotes |
and |
T1640 |
139-143 |
NN |
denotes |
MCAD |
T1641 |
143-144 |
SYM |
denotes |
+ |
T1642 |
144-145 |
HYPH |
denotes |
/ |
T1643 |
145-146 |
SYM |
denotes |
− |
T1635 |
147-151 |
NNS |
denotes |
mice |
T1644 |
151-153 |
, |
denotes |
, |
T1645 |
153-156 |
CC |
denotes |
and |
T1646 |
157-158 |
DT |
denotes |
a |
T1648 |
159-162 |
CD |
denotes |
1.5 |
T1650 |
162-163 |
HYPH |
denotes |
- |
T1649 |
163-165 |
NN |
denotes |
kb |
T1647 |
166-174 |
NN |
denotes |
fragment |
T1651 |
175-177 |
IN |
denotes |
in |
T1652 |
178-182 |
NN |
denotes |
MCAD |
T1654 |
182-183 |
SYM |
denotes |
− |
T1655 |
183-184 |
HYPH |
denotes |
/ |
T1656 |
184-185 |
SYM |
denotes |
− |
T1653 |
186-190 |
NNS |
denotes |
mice |
T1657 |
191-192 |
-LRB- |
denotes |
( |
T1659 |
192-196 |
NNS |
denotes |
data |
T1660 |
197-200 |
RB |
denotes |
not |
T1658 |
201-206 |
VBN |
denotes |
shown |
T1661 |
206-207 |
-RRB- |
denotes |
) |
T1662 |
207-208 |
. |
denotes |
. |
T1663 |
208-378 |
sentence |
denotes |
Sequence analysis of the 1.5-kb fragment revealed that the amplified fragment consisted of exon 7 to exon 10 with 280 bp of pGEM plasmid sequence followed by exons 8–11. |
T1664 |
209-217 |
NN |
denotes |
Sequence |
T1665 |
218-226 |
NN |
denotes |
analysis |
T1667 |
227-229 |
IN |
denotes |
of |
T1668 |
230-233 |
DT |
denotes |
the |
T1670 |
234-237 |
CD |
denotes |
1.5 |
T1672 |
237-238 |
HYPH |
denotes |
- |
T1671 |
238-240 |
NN |
denotes |
kb |
T1669 |
241-249 |
NN |
denotes |
fragment |
T1666 |
250-258 |
VBD |
denotes |
revealed |
T1673 |
259-263 |
IN |
denotes |
that |
T1675 |
264-267 |
DT |
denotes |
the |
T1677 |
268-277 |
VBN |
denotes |
amplified |
T1676 |
278-286 |
NN |
denotes |
fragment |
T1674 |
287-296 |
VBD |
denotes |
consisted |
T1678 |
297-299 |
IN |
denotes |
of |
T1679 |
300-304 |
NN |
denotes |
exon |
T1680 |
305-306 |
CD |
denotes |
7 |
T1681 |
307-309 |
IN |
denotes |
to |
T1682 |
310-314 |
NN |
denotes |
exon |
T1683 |
315-317 |
CD |
denotes |
10 |
T1684 |
318-322 |
IN |
denotes |
with |
T1685 |
323-326 |
CD |
denotes |
280 |
T1686 |
327-329 |
NN |
denotes |
bp |
T1687 |
330-332 |
IN |
denotes |
of |
T1688 |
333-337 |
NN |
denotes |
pGEM |
T1690 |
338-345 |
NN |
denotes |
plasmid |
T1689 |
346-354 |
NN |
denotes |
sequence |
T1691 |
355-363 |
VBN |
denotes |
followed |
T1692 |
364-366 |
IN |
denotes |
by |
T1693 |
367-372 |
NNS |
denotes |
exons |
T1694 |
373-374 |
CD |
denotes |
8 |
T1695 |
374-375 |
SYM |
denotes |
– |
T1696 |
375-377 |
CD |
denotes |
11 |
T1697 |
377-378 |
. |
denotes |
. |
T1698 |
378-481 |
sentence |
denotes |
Some of the plasmid sequences, along with the pPGKNEOpA sequence, were deleted from this spliced mRNA. |
T1699 |
379-383 |
DT |
denotes |
Some |
T1701 |
384-386 |
IN |
denotes |
of |
T1702 |
387-390 |
DT |
denotes |
the |
T1704 |
391-398 |
NN |
denotes |
plasmid |
T1703 |
399-408 |
NNS |
denotes |
sequences |
T1705 |
408-410 |
, |
denotes |
, |
T1706 |
410-415 |
IN |
denotes |
along |
T1707 |
416-420 |
IN |
denotes |
with |
T1708 |
421-424 |
DT |
denotes |
the |
T1710 |
425-434 |
NN |
denotes |
pPGKNEOpA |
T1709 |
435-443 |
NN |
denotes |
sequence |
T1711 |
443-445 |
, |
denotes |
, |
T1712 |
445-449 |
VBD |
denotes |
were |
T1700 |
450-457 |
VBN |
denotes |
deleted |
T1713 |
458-462 |
IN |
denotes |
from |
T1714 |
463-467 |
DT |
denotes |
this |
T1716 |
468-475 |
VBN |
denotes |
spliced |
T1715 |
476-480 |
NN |
denotes |
mRNA |
T1717 |
480-481 |
. |
denotes |
. |
T1718 |
481-754 |
sentence |
denotes |
Northern blot analysis revealed Acadm was normally expressed in all tissues analyzed from MCAD+/+ mice with the most robust expression occurring in brown fat, kidney, heart, skeletal muscle, and liver with minimal expression in the brain, white fat, and testes (Figure 2). |
T1719 |
482-490 |
NNP |
denotes |
Northern |
T1720 |
491-495 |
NN |
denotes |
blot |
T1721 |
496-504 |
NN |
denotes |
analysis |
T1722 |
505-513 |
VBD |
denotes |
revealed |
T1723 |
514-519 |
NN |
denotes |
Acadm |
T1725 |
520-523 |
VBD |
denotes |
was |
T1726 |
524-532 |
RB |
denotes |
normally |
T1724 |
533-542 |
VBN |
denotes |
expressed |
T1727 |
543-545 |
IN |
denotes |
in |
T1728 |
546-549 |
DT |
denotes |
all |
T1729 |
550-557 |
NNS |
denotes |
tissues |
T1730 |
558-566 |
VBN |
denotes |
analyzed |
T1731 |
567-571 |
IN |
denotes |
from |
T1732 |
572-576 |
NN |
denotes |
MCAD |
T1734 |
576-577 |
SYM |
denotes |
+ |
T1735 |
577-578 |
HYPH |
denotes |
/ |
T1736 |
578-579 |
SYM |
denotes |
+ |
T1733 |
580-584 |
NNS |
denotes |
mice |
T1737 |
585-589 |
IN |
denotes |
with |
T1738 |
590-593 |
DT |
denotes |
the |
T1740 |
594-598 |
RBS |
denotes |
most |
T1741 |
599-605 |
JJ |
denotes |
robust |
T1739 |
606-616 |
NN |
denotes |
expression |
T1742 |
617-626 |
VBG |
denotes |
occurring |
T1743 |
627-629 |
IN |
denotes |
in |
T1744 |
630-635 |
JJ |
denotes |
brown |
T1745 |
636-639 |
NN |
denotes |
fat |
T1746 |
639-641 |
, |
denotes |
, |
T1747 |
641-647 |
NN |
denotes |
kidney |
T1748 |
647-649 |
, |
denotes |
, |
T1749 |
649-654 |
NN |
denotes |
heart |
T1750 |
654-656 |
, |
denotes |
, |
T1751 |
656-664 |
JJ |
denotes |
skeletal |
T1752 |
665-671 |
NN |
denotes |
muscle |
T1753 |
671-673 |
, |
denotes |
, |
T1754 |
673-676 |
CC |
denotes |
and |
T1755 |
677-682 |
NN |
denotes |
liver |
T1756 |
683-687 |
IN |
denotes |
with |
T1757 |
688-695 |
JJ |
denotes |
minimal |
T1758 |
696-706 |
NN |
denotes |
expression |
T1759 |
707-709 |
IN |
denotes |
in |
T1760 |
710-713 |
DT |
denotes |
the |
T1761 |
714-719 |
NN |
denotes |
brain |
T1762 |
719-721 |
, |
denotes |
, |
T1763 |
721-726 |
JJ |
denotes |
white |
T1764 |
727-730 |
NN |
denotes |
fat |
T1765 |
730-732 |
, |
denotes |
, |
T1766 |
732-735 |
CC |
denotes |
and |
T1767 |
736-742 |
NNS |
denotes |
testes |
T1768 |
743-744 |
-LRB- |
denotes |
( |
T1769 |
744-750 |
NN |
denotes |
Figure |
T1770 |
751-752 |
CD |
denotes |
2 |
T1771 |
752-753 |
-RRB- |
denotes |
) |
T1772 |
753-754 |
. |
denotes |
. |
T1773 |
754-916 |
sentence |
denotes |
Interestingly, although RT-PCR amplified an incorrectly spliced Acadm transcript, no Acadm transcripts were detected by northern blot analysis from MCAD−/− mice. |
T1774 |
755-768 |
RB |
denotes |
Interestingly |
T1776 |
768-770 |
, |
denotes |
, |
T1777 |
770-778 |
IN |
denotes |
although |
T1779 |
779-781 |
NN |
denotes |
RT |
T1781 |
781-782 |
HYPH |
denotes |
- |
T1780 |
782-785 |
NN |
denotes |
PCR |
T1778 |
786-795 |
VBD |
denotes |
amplified |
T1782 |
796-798 |
DT |
denotes |
an |
T1784 |
799-810 |
RB |
denotes |
incorrectly |
T1785 |
811-818 |
VBN |
denotes |
spliced |
T1786 |
819-824 |
NN |
denotes |
Acadm |
T1783 |
825-835 |
NN |
denotes |
transcript |
T1787 |
835-837 |
, |
denotes |
, |
T1788 |
837-839 |
DT |
denotes |
no |
T1790 |
840-845 |
NN |
denotes |
Acadm |
T1789 |
846-857 |
NNS |
denotes |
transcripts |
T1791 |
858-862 |
VBD |
denotes |
were |
T1775 |
863-871 |
VBN |
denotes |
detected |
T1792 |
872-874 |
IN |
denotes |
by |
T1793 |
875-883 |
NNP |
denotes |
northern |
T1794 |
884-888 |
NN |
denotes |
blot |
T1795 |
889-897 |
NN |
denotes |
analysis |
T1796 |
898-902 |
IN |
denotes |
from |
T1797 |
903-907 |
NN |
denotes |
MCAD |
T1799 |
907-908 |
SYM |
denotes |
− |
T1800 |
908-909 |
HYPH |
denotes |
/ |
T1801 |
909-910 |
SYM |
denotes |
− |
T1798 |
911-915 |
NNS |
denotes |
mice |
T1802 |
915-916 |
. |
denotes |
. |
T1803 |
916-1058 |
sentence |
denotes |
These results strongly suggest that the mutant RNA is unstable and degraded rapidly or, alternatively, undergoes nonsense mediated RNA decay. |
T1804 |
917-922 |
DT |
denotes |
These |
T1805 |
923-930 |
NNS |
denotes |
results |
T1807 |
931-939 |
RB |
denotes |
strongly |
T1806 |
940-947 |
VBP |
denotes |
suggest |
T1808 |
948-952 |
IN |
denotes |
that |
T1810 |
953-956 |
DT |
denotes |
the |
T1812 |
957-963 |
NN |
denotes |
mutant |
T1811 |
964-967 |
NN |
denotes |
RNA |
T1809 |
968-970 |
VBZ |
denotes |
is |
T1813 |
971-979 |
JJ |
denotes |
unstable |
T1814 |
980-983 |
CC |
denotes |
and |
T1815 |
984-992 |
VBN |
denotes |
degraded |
T1816 |
993-1000 |
RB |
denotes |
rapidly |
T1817 |
1001-1003 |
CC |
denotes |
or |
T1818 |
1003-1005 |
, |
denotes |
, |
T1819 |
1005-1018 |
RB |
denotes |
alternatively |
T1821 |
1018-1020 |
, |
denotes |
, |
T1820 |
1020-1029 |
VBZ |
denotes |
undergoes |
T1822 |
1030-1038 |
NN |
denotes |
nonsense |
T1823 |
1039-1047 |
VBN |
denotes |
mediated |
T1825 |
1048-1051 |
NN |
denotes |
RNA |
T1824 |
1052-1057 |
NN |
denotes |
decay |
T1826 |
1057-1058 |
. |
denotes |
. |
T7455 |
1069-1077 |
NNP |
denotes |
Northern |
T7456 |
1078-1082 |
NN |
denotes |
Blot |
T7457 |
1083-1091 |
NN |
denotes |
Analysis |
T7458 |
1092-1096 |
IN |
denotes |
from |
T7459 |
1097-1101 |
NN |
denotes |
MCAD |
T7461 |
1101-1102 |
SYM |
denotes |
− |
T7462 |
1102-1103 |
HYPH |
denotes |
/ |
T7463 |
1103-1104 |
SYM |
denotes |
− |
T7464 |
1105-1106 |
-LRB- |
denotes |
( |
T7466 |
1106-1107 |
NN |
denotes |
n |
T7467 |
1108-1109 |
SYM |
denotes |
= |
T7465 |
1110-1111 |
CD |
denotes |
2 |
T7468 |
1111-1112 |
-RRB- |
denotes |
) |
T7469 |
1113-1116 |
CC |
denotes |
and |
T7470 |
1117-1121 |
NN |
denotes |
MCAD |
T7471 |
1121-1122 |
SYM |
denotes |
+ |
T7472 |
1122-1123 |
HYPH |
denotes |
/ |
T7473 |
1123-1124 |
SYM |
denotes |
+ |
T7474 |
1125-1126 |
-LRB- |
denotes |
( |
T7476 |
1126-1127 |
NN |
denotes |
n |
T7477 |
1128-1129 |
SYM |
denotes |
= |
T7475 |
1130-1131 |
CD |
denotes |
2 |
T7478 |
1131-1132 |
-RRB- |
denotes |
) |
T7460 |
1133-1137 |
NNS |
denotes |
Mice |
T7479 |
1137-1289 |
sentence |
denotes |
Acadm message was detected from the heart, liver, brown fat, brain, kidney, and muscle (and white fat and testes, data not shown) of only MCAD+/+ mice. |
T7480 |
1138-1143 |
NN |
denotes |
Acadm |
T7481 |
1144-1151 |
NN |
denotes |
message |
T7483 |
1152-1155 |
VBD |
denotes |
was |
T7482 |
1156-1164 |
VBN |
denotes |
detected |
T7484 |
1165-1169 |
IN |
denotes |
from |
T7485 |
1170-1173 |
DT |
denotes |
the |
T7486 |
1174-1179 |
NN |
denotes |
heart |
T7487 |
1179-1181 |
, |
denotes |
, |
T7488 |
1181-1186 |
NN |
denotes |
liver |
T7489 |
1186-1188 |
, |
denotes |
, |
T7490 |
1188-1193 |
JJ |
denotes |
brown |
T7491 |
1194-1197 |
NN |
denotes |
fat |
T7492 |
1197-1199 |
, |
denotes |
, |
T7493 |
1199-1204 |
NN |
denotes |
brain |
T7494 |
1204-1206 |
, |
denotes |
, |
T7495 |
1206-1212 |
NN |
denotes |
kidney |
T7496 |
1212-1214 |
, |
denotes |
, |
T7497 |
1214-1217 |
CC |
denotes |
and |
T7498 |
1218-1224 |
NN |
denotes |
muscle |
T7499 |
1225-1226 |
-LRB- |
denotes |
( |
T7500 |
1226-1229 |
CC |
denotes |
and |
T7501 |
1230-1235 |
JJ |
denotes |
white |
T7502 |
1236-1239 |
NN |
denotes |
fat |
T7503 |
1240-1243 |
CC |
denotes |
and |
T7504 |
1244-1250 |
NNS |
denotes |
testes |
T7505 |
1250-1252 |
, |
denotes |
, |
T7507 |
1252-1256 |
NNS |
denotes |
data |
T7508 |
1257-1260 |
RB |
denotes |
not |
T7506 |
1261-1266 |
VBN |
denotes |
shown |
T7509 |
1266-1267 |
-RRB- |
denotes |
) |
T7510 |
1268-1270 |
IN |
denotes |
of |
T7511 |
1271-1275 |
JJ |
denotes |
only |
T7513 |
1276-1280 |
NN |
denotes |
MCAD |
T7514 |
1280-1281 |
SYM |
denotes |
+ |
T7515 |
1281-1282 |
HYPH |
denotes |
/ |
T7516 |
1282-1283 |
SYM |
denotes |
+ |
T7512 |
1284-1288 |
NNS |
denotes |
mice |
T7517 |
1288-1289 |
. |
denotes |
. |
T7518 |
1289-1371 |
sentence |
denotes |
Most robust expression occurred in brown fat, kidney, heart, and skeletal muscle. |
T7519 |
1290-1294 |
RBS |
denotes |
Most |
T7521 |
1295-1301 |
JJ |
denotes |
robust |
T7520 |
1302-1312 |
NN |
denotes |
expression |
T7522 |
1313-1321 |
VBD |
denotes |
occurred |
T7523 |
1322-1324 |
IN |
denotes |
in |
T7524 |
1325-1330 |
JJ |
denotes |
brown |
T7525 |
1331-1334 |
NN |
denotes |
fat |
T7526 |
1334-1336 |
, |
denotes |
, |
T7527 |
1336-1342 |
NN |
denotes |
kidney |
T7528 |
1342-1344 |
, |
denotes |
, |
T7529 |
1344-1349 |
NN |
denotes |
heart |
T7530 |
1349-1351 |
, |
denotes |
, |
T7531 |
1351-1354 |
CC |
denotes |
and |
T7532 |
1355-1363 |
JJ |
denotes |
skeletal |
T7533 |
1364-1370 |
NN |
denotes |
muscle |
T7534 |
1370-1371 |
. |
denotes |
. |
T7535 |
1371-1435 |
sentence |
denotes |
MCAD−/− mice had no detectable message in all tissues examined. |
T7536 |
1372-1376 |
NN |
denotes |
MCAD |
T7538 |
1376-1377 |
SYM |
denotes |
− |
T7539 |
1377-1378 |
HYPH |
denotes |
/ |
T7540 |
1378-1379 |
SYM |
denotes |
− |
T7537 |
1380-1384 |
NNS |
denotes |
mice |
T7541 |
1385-1388 |
VBD |
denotes |
had |
T7542 |
1389-1391 |
DT |
denotes |
no |
T7544 |
1392-1402 |
JJ |
denotes |
detectable |
T7543 |
1403-1410 |
NN |
denotes |
message |
T7545 |
1411-1413 |
IN |
denotes |
in |
T7546 |
1414-1417 |
DT |
denotes |
all |
T7547 |
1418-1425 |
NNS |
denotes |
tissues |
T7548 |
1426-1434 |
VBN |
denotes |
examined |
T7549 |
1434-1435 |
. |
denotes |
. |
R1025 |
T1606 |
T1607 |
compound |
RNA,Analysis |
R1026 |
T1609 |
T1610 |
compound |
RT,PCR |
R1027 |
T1610 |
T1612 |
compound |
PCR,amplification |
R1028 |
T1611 |
T1610 |
punct |
-,PCR |
R1029 |
T1612 |
T1613 |
nsubj |
amplification,amplified |
R1030 |
T1614 |
T1612 |
prep |
from,amplification |
R1031 |
T1615 |
T1616 |
nmod |
exon,7 |
R1032 |
T1616 |
T1614 |
pobj |
7,from |
R1033 |
T1617 |
T1616 |
prep |
to,7 |
R1034 |
T1618 |
T1617 |
pobj |
11,to |
R1035 |
T1619 |
T1612 |
prep |
from,amplification |
R1036 |
T1620 |
T1621 |
amod |
total,RNA |
R1037 |
T1621 |
T1619 |
pobj |
RNA,from |
R1038 |
T1622 |
T1621 |
compound |
heart,RNA |
R1039 |
T1623 |
T1624 |
det |
the,fragment |
R1040 |
T1624 |
T1613 |
dobj |
fragment,amplified |
R1041 |
T1625 |
T1624 |
amod |
expected,fragment |
R1042 |
T1626 |
T1627 |
nummod |
600,pair |
R1043 |
T1627 |
T1624 |
nmod |
pair,fragment |
R1044 |
T1628 |
T1627 |
punct |
-,pair |
R1045 |
T1629 |
T1627 |
nmod |
base,pair |
R1046 |
T1630 |
T1627 |
punct |
(,pair |
R1047 |
T1631 |
T1627 |
appos |
bp,pair |
R1048 |
T1632 |
T1624 |
punct |
),fragment |
R1049 |
T1633 |
T1624 |
prep |
in,fragment |
R1050 |
T1634 |
T1635 |
nmod |
MCAD,mice |
R1051 |
T1635 |
T1633 |
pobj |
mice,in |
R1052 |
T1636 |
T1634 |
punct |
+,MCAD |
R1053 |
T1637 |
T1634 |
punct |
/,MCAD |
R1054 |
T1638 |
T1634 |
punct |
+,MCAD |
R1055 |
T1639 |
T1634 |
cc |
and,MCAD |
R1056 |
T1640 |
T1634 |
conj |
MCAD,MCAD |
R1057 |
T1641 |
T1640 |
punct |
+,MCAD |
R1058 |
T1642 |
T1640 |
punct |
/,MCAD |
R1059 |
T1643 |
T1640 |
punct |
−,MCAD |
R1060 |
T1644 |
T1624 |
punct |
", ",fragment |
R1061 |
T1645 |
T1624 |
cc |
and,fragment |
R1062 |
T1646 |
T1647 |
det |
a,fragment |
R1063 |
T1647 |
T1624 |
conj |
fragment,fragment |
R1064 |
T1648 |
T1649 |
nummod |
1.5,kb |
R1065 |
T1649 |
T1647 |
compound |
kb,fragment |
R1066 |
T1650 |
T1649 |
punct |
-,kb |
R1067 |
T1651 |
T1647 |
prep |
in,fragment |
R1068 |
T1652 |
T1653 |
nmod |
MCAD,mice |
R1069 |
T1653 |
T1651 |
pobj |
mice,in |
R1070 |
T1654 |
T1652 |
punct |
−,MCAD |
R1071 |
T1655 |
T1652 |
punct |
/,MCAD |
R1072 |
T1656 |
T1652 |
punct |
−,MCAD |
R1073 |
T1657 |
T1658 |
punct |
(,shown |
R1074 |
T1658 |
T1613 |
parataxis |
shown,amplified |
R1075 |
T1659 |
T1658 |
nsubj |
data,shown |
R1076 |
T1660 |
T1658 |
neg |
not,shown |
R1077 |
T1661 |
T1658 |
punct |
),shown |
R1078 |
T1662 |
T1613 |
punct |
.,amplified |
R1079 |
T1664 |
T1665 |
compound |
Sequence,analysis |
R1080 |
T1665 |
T1666 |
nsubj |
analysis,revealed |
R1081 |
T1667 |
T1665 |
prep |
of,analysis |
R1082 |
T1668 |
T1669 |
det |
the,fragment |
R1083 |
T1669 |
T1667 |
pobj |
fragment,of |
R1084 |
T1670 |
T1671 |
nummod |
1.5,kb |
R1085 |
T1671 |
T1669 |
compound |
kb,fragment |
R1086 |
T1672 |
T1671 |
punct |
-,kb |
R1087 |
T1673 |
T1674 |
mark |
that,consisted |
R1088 |
T1674 |
T1666 |
ccomp |
consisted,revealed |
R1089 |
T1675 |
T1676 |
det |
the,fragment |
R1090 |
T1676 |
T1674 |
nsubj |
fragment,consisted |
R1091 |
T1677 |
T1676 |
amod |
amplified,fragment |
R1092 |
T1678 |
T1674 |
prep |
of,consisted |
R1093 |
T1679 |
T1678 |
pobj |
exon,of |
R1094 |
T1680 |
T1679 |
nummod |
7,exon |
R1095 |
T1681 |
T1679 |
prep |
to,exon |
R1096 |
T1682 |
T1681 |
pobj |
exon,to |
R1097 |
T1683 |
T1682 |
nummod |
10,exon |
R1098 |
T1684 |
T1674 |
prep |
with,consisted |
R1099 |
T1685 |
T1686 |
nummod |
280,bp |
R1100 |
T1686 |
T1684 |
pobj |
bp,with |
R1101 |
T1687 |
T1686 |
prep |
of,bp |
R1102 |
T1688 |
T1689 |
compound |
pGEM,sequence |
R1103 |
T1689 |
T1687 |
pobj |
sequence,of |
R1104 |
T1690 |
T1689 |
compound |
plasmid,sequence |
R1105 |
T1691 |
T1689 |
acl |
followed,sequence |
R1106 |
T1692 |
T1691 |
agent |
by,followed |
R1107 |
T1693 |
T1694 |
nmod |
exons,8 |
R1108 |
T1694 |
T1692 |
pobj |
8,by |
R1109 |
T1695 |
T1696 |
punct |
–,11 |
R1110 |
T1696 |
T1694 |
prep |
11,8 |
R1111 |
T1697 |
T1666 |
punct |
.,revealed |
R1112 |
T1699 |
T1700 |
nsubjpass |
Some,deleted |
R1113 |
T1701 |
T1699 |
prep |
of,Some |
R1114 |
T1702 |
T1703 |
det |
the,sequences |
R1115 |
T1703 |
T1701 |
pobj |
sequences,of |
R1116 |
T1704 |
T1703 |
compound |
plasmid,sequences |
R1117 |
T1705 |
T1699 |
punct |
", ",Some |
R1118 |
T1706 |
T1699 |
cc |
along,Some |
R1119 |
T1707 |
T1706 |
dep |
with,along |
R1120 |
T1708 |
T1709 |
det |
the,sequence |
R1121 |
T1709 |
T1699 |
conj |
sequence,Some |
R1122 |
T1710 |
T1709 |
compound |
pPGKNEOpA,sequence |
R1123 |
T1711 |
T1700 |
punct |
", ",deleted |
R1124 |
T1712 |
T1700 |
auxpass |
were,deleted |
R1125 |
T1713 |
T1700 |
prep |
from,deleted |
R1126 |
T1714 |
T1715 |
det |
this,mRNA |
R1127 |
T1715 |
T1713 |
pobj |
mRNA,from |
R1128 |
T1716 |
T1715 |
amod |
spliced,mRNA |
R1129 |
T1717 |
T1700 |
punct |
.,deleted |
R1130 |
T1719 |
T1720 |
compound |
Northern,blot |
R1131 |
T1720 |
T1721 |
compound |
blot,analysis |
R1132 |
T1721 |
T1722 |
nsubj |
analysis,revealed |
R1133 |
T1723 |
T1724 |
nsubjpass |
Acadm,expressed |
R1134 |
T1724 |
T1722 |
advcl |
expressed,revealed |
R1135 |
T1725 |
T1724 |
auxpass |
was,expressed |
R1136 |
T1726 |
T1724 |
advmod |
normally,expressed |
R1137 |
T1727 |
T1724 |
prep |
in,expressed |
R1138 |
T1728 |
T1729 |
det |
all,tissues |
R1139 |
T1729 |
T1727 |
pobj |
tissues,in |
R1140 |
T1730 |
T1729 |
acl |
analyzed,tissues |
R1141 |
T1731 |
T1729 |
prep |
from,tissues |
R1142 |
T1732 |
T1733 |
nmod |
MCAD,mice |
R1143 |
T1733 |
T1731 |
pobj |
mice,from |
R1144 |
T1734 |
T1732 |
punct |
+,MCAD |
R1145 |
T1735 |
T1732 |
punct |
/,MCAD |
R1146 |
T1736 |
T1732 |
punct |
+,MCAD |
R1147 |
T1737 |
T1724 |
prep |
with,expressed |
R1148 |
T1738 |
T1739 |
det |
the,expression |
R1149 |
T1739 |
T1742 |
nsubj |
expression,occurring |
R1150 |
T1740 |
T1741 |
advmod |
most,robust |
R1151 |
T1741 |
T1739 |
amod |
robust,expression |
R1152 |
T1742 |
T1737 |
pcomp |
occurring,with |
R1153 |
T1743 |
T1742 |
prep |
in,occurring |
R1154 |
T1744 |
T1745 |
amod |
brown,fat |
R1155 |
T1745 |
T1743 |
pobj |
fat,in |
R1156 |
T1746 |
T1745 |
punct |
", ",fat |
R1157 |
T1747 |
T1745 |
conj |
kidney,fat |
R1158 |
T1748 |
T1747 |
punct |
", ",kidney |
R1159 |
T1749 |
T1747 |
conj |
heart,kidney |
R1160 |
T1750 |
T1749 |
punct |
", ",heart |
R1161 |
T1751 |
T1752 |
amod |
skeletal,muscle |
R1162 |
T1752 |
T1749 |
conj |
muscle,heart |
R1163 |
T1753 |
T1752 |
punct |
", ",muscle |
R1164 |
T1754 |
T1752 |
cc |
and,muscle |
R1165 |
T1755 |
T1752 |
conj |
liver,muscle |
R1166 |
T1756 |
T1724 |
prep |
with,expressed |
R1167 |
T1757 |
T1758 |
amod |
minimal,expression |
R1168 |
T1758 |
T1756 |
pobj |
expression,with |
R1169 |
T1759 |
T1758 |
prep |
in,expression |
R1170 |
T1760 |
T1761 |
det |
the,brain |
R1171 |
T1761 |
T1759 |
pobj |
brain,in |
R1172 |
T1762 |
T1761 |
punct |
", ",brain |
R1173 |
T1763 |
T1764 |
amod |
white,fat |
R1174 |
T1764 |
T1761 |
conj |
fat,brain |
R1175 |
T1765 |
T1764 |
punct |
", ",fat |
R1176 |
T1766 |
T1764 |
cc |
and,fat |
R1177 |
T1767 |
T1764 |
conj |
testes,fat |
R1178 |
T1768 |
T1769 |
punct |
(,Figure |
R1179 |
T1769 |
T1722 |
parataxis |
Figure,revealed |
R1180 |
T1770 |
T1769 |
nummod |
2,Figure |
R1181 |
T1771 |
T1769 |
punct |
),Figure |
R1182 |
T1772 |
T1722 |
punct |
.,revealed |
R1183 |
T1774 |
T1775 |
advmod |
Interestingly,detected |
R1184 |
T1776 |
T1775 |
punct |
", ",detected |
R1185 |
T1777 |
T1778 |
mark |
although,amplified |
R1186 |
T1778 |
T1775 |
advcl |
amplified,detected |
R1187 |
T1779 |
T1780 |
compound |
RT,PCR |
R1188 |
T1780 |
T1778 |
nsubj |
PCR,amplified |
R1189 |
T1781 |
T1780 |
punct |
-,PCR |
R1190 |
T1782 |
T1783 |
det |
an,transcript |
R1191 |
T1783 |
T1778 |
dobj |
transcript,amplified |
R1192 |
T1784 |
T1785 |
advmod |
incorrectly,spliced |
R1193 |
T1785 |
T1783 |
amod |
spliced,transcript |
R1194 |
T1786 |
T1783 |
compound |
Acadm,transcript |
R1195 |
T1787 |
T1775 |
punct |
", ",detected |
R1196 |
T1788 |
T1789 |
det |
no,transcripts |
R1197 |
T1789 |
T1775 |
nsubjpass |
transcripts,detected |
R1198 |
T1790 |
T1789 |
compound |
Acadm,transcripts |
R1199 |
T1791 |
T1775 |
auxpass |
were,detected |
R1200 |
T1792 |
T1775 |
prep |
by,detected |
R1201 |
T1793 |
T1794 |
compound |
northern,blot |
R1202 |
T1794 |
T1795 |
compound |
blot,analysis |
R1203 |
T1795 |
T1792 |
pobj |
analysis,by |
R1204 |
T1796 |
T1775 |
prep |
from,detected |
R1205 |
T1797 |
T1798 |
nmod |
MCAD,mice |
R1206 |
T1798 |
T1796 |
pobj |
mice,from |
R1207 |
T1799 |
T1797 |
punct |
−,MCAD |
R1208 |
T1800 |
T1797 |
punct |
/,MCAD |
R1209 |
T1801 |
T1797 |
punct |
−,MCAD |
R1210 |
T1802 |
T1775 |
punct |
.,detected |
R1211 |
T1804 |
T1805 |
det |
These,results |
R1212 |
T1805 |
T1806 |
nsubj |
results,suggest |
R1213 |
T1807 |
T1806 |
advmod |
strongly,suggest |
R1214 |
T1808 |
T1809 |
mark |
that,is |
R1215 |
T1809 |
T1806 |
ccomp |
is,suggest |
R1216 |
T1810 |
T1811 |
det |
the,RNA |
R1217 |
T1811 |
T1809 |
nsubj |
RNA,is |
R1218 |
T1812 |
T1811 |
compound |
mutant,RNA |
R1219 |
T1813 |
T1809 |
acomp |
unstable,is |
R1220 |
T1814 |
T1809 |
cc |
and,is |
R1221 |
T1815 |
T1809 |
conj |
degraded,is |
R1222 |
T1816 |
T1815 |
advmod |
rapidly,degraded |
R1223 |
T1817 |
T1809 |
cc |
or,is |
R1224 |
T1818 |
T1809 |
punct |
", ",is |
R1225 |
T1819 |
T1820 |
advmod |
alternatively,undergoes |
R1226 |
T1820 |
T1809 |
conj |
undergoes,is |
R1227 |
T1821 |
T1820 |
punct |
", ",undergoes |
R1228 |
T1822 |
T1823 |
npadvmod |
nonsense,mediated |
R1229 |
T1823 |
T1824 |
amod |
mediated,decay |
R1230 |
T1824 |
T1820 |
dobj |
decay,undergoes |
R1231 |
T1825 |
T1824 |
compound |
RNA,decay |
R1232 |
T1826 |
T1806 |
punct |
.,suggest |
R4817 |
T7455 |
T7456 |
compound |
Northern,Blot |
R4818 |
T7456 |
T7457 |
compound |
Blot,Analysis |
R4819 |
T7458 |
T7457 |
prep |
from,Analysis |
R4820 |
T7459 |
T7460 |
nmod |
MCAD,Mice |
R4821 |
T7460 |
T7458 |
pobj |
Mice,from |
R4822 |
T7461 |
T7459 |
punct |
−,MCAD |
R4823 |
T7462 |
T7459 |
punct |
/,MCAD |
R4824 |
T7463 |
T7459 |
punct |
−,MCAD |
R4825 |
T7464 |
T7465 |
punct |
(,2 |
R4826 |
T7465 |
T7459 |
parataxis |
2,MCAD |
R4827 |
T7466 |
T7465 |
nsubj |
n,2 |
R4828 |
T7467 |
T7465 |
punct |
=,2 |
R4829 |
T7468 |
T7465 |
punct |
),2 |
R4830 |
T7469 |
T7459 |
cc |
and,MCAD |
R4831 |
T7470 |
T7459 |
conj |
MCAD,MCAD |
R4832 |
T7471 |
T7470 |
punct |
+,MCAD |
R4833 |
T7472 |
T7470 |
punct |
/,MCAD |
R4834 |
T7473 |
T7470 |
punct |
+,MCAD |
R4835 |
T7474 |
T7475 |
punct |
(,2 |
R4836 |
T7475 |
T7470 |
parataxis |
2,MCAD |
R4837 |
T7476 |
T7475 |
nsubj |
n,2 |
R4838 |
T7477 |
T7475 |
punct |
=,2 |
R4839 |
T7478 |
T7475 |
punct |
),2 |
R4840 |
T7480 |
T7481 |
compound |
Acadm,message |
R4841 |
T7481 |
T7482 |
nsubjpass |
message,detected |
R4842 |
T7483 |
T7482 |
auxpass |
was,detected |
R4843 |
T7484 |
T7482 |
prep |
from,detected |
R4844 |
T7485 |
T7486 |
det |
the,heart |
R4845 |
T7486 |
T7484 |
pobj |
heart,from |
R4846 |
T7487 |
T7486 |
punct |
", ",heart |
R4847 |
T7488 |
T7486 |
conj |
liver,heart |
R4848 |
T7489 |
T7488 |
punct |
", ",liver |
R4849 |
T7490 |
T7491 |
amod |
brown,fat |
R4850 |
T7491 |
T7488 |
conj |
fat,liver |
R4851 |
T7492 |
T7491 |
punct |
", ",fat |
R4852 |
T7493 |
T7491 |
conj |
brain,fat |
R4853 |
T7494 |
T7493 |
punct |
", ",brain |
R4854 |
T7495 |
T7493 |
conj |
kidney,brain |
R4855 |
T7496 |
T7495 |
punct |
", ",kidney |
R4856 |
T7497 |
T7495 |
cc |
and,kidney |
R4857 |
T7498 |
T7495 |
conj |
muscle,kidney |
R4858 |
T7499 |
T7486 |
punct |
(,heart |
R4859 |
T7500 |
T7486 |
cc |
and,heart |
R4860 |
T7501 |
T7502 |
amod |
white,fat |
R4861 |
T7502 |
T7486 |
conj |
fat,heart |
R4862 |
T7503 |
T7502 |
cc |
and,fat |
R4863 |
T7504 |
T7502 |
conj |
testes,fat |
R4864 |
T7505 |
T7506 |
punct |
", ",shown |
R4865 |
T7506 |
T7486 |
parataxis |
shown,heart |
R4866 |
T7507 |
T7506 |
nsubj |
data,shown |
R4867 |
T7508 |
T7506 |
neg |
not,shown |
R4868 |
T7509 |
T7506 |
punct |
),shown |
R4869 |
T7510 |
T7486 |
prep |
of,heart |
R4870 |
T7511 |
T7512 |
amod |
only,mice |
R4871 |
T7512 |
T7510 |
pobj |
mice,of |
R4872 |
T7513 |
T7512 |
nmod |
MCAD,mice |
R4873 |
T7514 |
T7513 |
punct |
+,MCAD |
R4874 |
T7515 |
T7513 |
punct |
/,MCAD |
R4875 |
T7516 |
T7513 |
punct |
+,MCAD |
R4876 |
T7517 |
T7482 |
punct |
.,detected |
R4877 |
T7519 |
T7520 |
advmod |
Most,expression |
R4878 |
T7520 |
T7522 |
nsubj |
expression,occurred |
R4879 |
T7521 |
T7520 |
amod |
robust,expression |
R4880 |
T7523 |
T7522 |
prep |
in,occurred |
R4881 |
T7524 |
T7525 |
amod |
brown,fat |
R4882 |
T7525 |
T7523 |
pobj |
fat,in |
R4883 |
T7526 |
T7525 |
punct |
", ",fat |
R4884 |
T7527 |
T7525 |
conj |
kidney,fat |
R4885 |
T7528 |
T7527 |
punct |
", ",kidney |
R4886 |
T7529 |
T7527 |
conj |
heart,kidney |
R4887 |
T7530 |
T7529 |
punct |
", ",heart |
R4888 |
T7531 |
T7529 |
cc |
and,heart |
R4889 |
T7532 |
T7533 |
amod |
skeletal,muscle |
R4890 |
T7533 |
T7529 |
conj |
muscle,heart |
R4891 |
T7534 |
T7522 |
punct |
.,occurred |
R4892 |
T7536 |
T7537 |
nmod |
MCAD,mice |
R4893 |
T7537 |
T7541 |
nsubj |
mice,had |
R4894 |
T7538 |
T7536 |
punct |
−,MCAD |
R4895 |
T7539 |
T7536 |
punct |
/,MCAD |
R4896 |
T7540 |
T7536 |
punct |
−,MCAD |
R4897 |
T7542 |
T7543 |
det |
no,message |
R4898 |
T7543 |
T7541 |
dobj |
message,had |
R4899 |
T7544 |
T7543 |
amod |
detectable,message |
R4900 |
T7545 |
T7541 |
prep |
in,had |
R4901 |
T7546 |
T7547 |
det |
all,tissues |
R4902 |
T7547 |
T7545 |
pobj |
tissues,in |
R4903 |
T7548 |
T7547 |
acl |
examined,tissues |
R4904 |
T7549 |
T7541 |
punct |
.,had |