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PMC:1183529 / 30654-31267 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE-old TextAE

Id Subject Object Predicate Lexical cue
T16011 0-3 NN denotes RNA
T16013 4-7 VBD denotes was
T16012 8-17 VBN denotes extracted
T16014 18-20 IN denotes by
T16015 21-29 JJ denotes standard
T16016 30-40 NNS denotes techniques
T16017 41-45 IN denotes from
T16018 46-54 JJ denotes thoracic
T16020 55-64 JJ denotes embryonic
T16019 65-71 NN denotes tissue
T16021 71-72 . denotes .
T16022 72-148 sentence denotes RT-PCR was performed using six primer sets designed to cover the Fog2 gene.
T16023 73-75 NN denotes RT
T16025 75-76 HYPH denotes -
T16024 76-79 NN denotes PCR
T16027 80-83 VBD denotes was
T16026 84-93 VBN denotes performed
T16028 94-99 VBG denotes using
T16029 100-103 CD denotes six
T16031 104-110 NN denotes primer
T16030 111-115 NNS denotes sets
T16032 116-124 VBN denotes designed
T16033 125-127 TO denotes to
T16034 128-133 VB denotes cover
T16035 134-137 DT denotes the
T16037 138-142 NN denotes Fog2
T16036 143-147 NN denotes gene
T16038 147-148 . denotes .
T16039 148-347 sentence denotes RT-PCR was repeated with radiolabeled primers to amplify an abnormally spliced region of the gene (Table S1), and the product was run on a denaturing sequencing gel according to standard techniques.
T16040 149-151 NN denotes RT
T16042 151-152 HYPH denotes -
T16041 152-155 NN denotes PCR
T16044 156-159 VBD denotes was
T16043 160-168 VBN denotes repeated
T16045 169-173 IN denotes with
T16046 174-186 VBN denotes radiolabeled
T16047 187-194 NNS denotes primers
T16048 195-197 TO denotes to
T16049 198-205 VB denotes amplify
T16050 206-208 DT denotes an
T16052 209-219 RB denotes abnormally
T16053 220-227 VBN denotes spliced
T16051 228-234 NN denotes region
T16054 235-237 IN denotes of
T16055 238-241 DT denotes the
T16056 242-246 NN denotes gene
T16057 247-248 -LRB- denotes (
T16059 248-253 NN denotes Table
T16058 254-256 NN denotes S1
T16060 256-257 -RRB- denotes )
T16061 257-259 , denotes ,
T16062 259-262 CC denotes and
T16063 263-266 DT denotes the
T16064 267-274 NN denotes product
T16066 275-278 VBD denotes was
T16065 279-282 VBN denotes run
T16067 283-285 IN denotes on
T16068 286-287 DT denotes a
T16070 288-298 VBG denotes denaturing
T16071 299-309 NN denotes sequencing
T16069 310-313 NN denotes gel
T16072 314-323 VBG denotes according
T16073 324-326 IN denotes to
T16074 327-335 JJ denotes standard
T16075 336-346 NNS denotes techniques
T16076 346-347 . denotes .
T16077 347-515 sentence denotes The RT-PCR product was cloned into pCR2.1 vector using TOPO TA Cloning Kit (Invitrogen, Carlsbad, California, United States) and sequenced using gene-specific primers.
T16078 348-351 DT denotes The
T16080 352-354 NN denotes RT
T16082 354-355 HYPH denotes -
T16081 355-358 NN denotes PCR
T16079 359-366 NN denotes product
T16084 367-370 VBD denotes was
T16083 371-377 VBN denotes cloned
T16085 378-382 IN denotes into
T16086 383-389 NN denotes pCR2.1
T16087 390-396 NN denotes vector
T16088 397-402 VBG denotes using
T16089 403-407 NN denotes TOPO
T16091 408-410 NN denotes TA
T16092 411-418 NNP denotes Cloning
T16090 419-422 NNP denotes Kit
T16093 423-424 -LRB- denotes (
T16094 424-434 NNP denotes Invitrogen
T16095 434-436 , denotes ,
T16096 436-444 NNP denotes Carlsbad
T16097 444-446 , denotes ,
T16098 446-456 NNP denotes California
T16099 456-458 , denotes ,
T16100 458-464 NNP denotes United
T16101 465-471 NNP denotes States
T16102 471-472 -RRB- denotes )
T16103 473-476 CC denotes and
T16104 477-486 VBN denotes sequenced
T16105 487-492 VBG denotes using
T16106 493-497 NN denotes gene
T16108 497-498 HYPH denotes -
T16107 498-506 JJ denotes specific
T16109 507-514 NNS denotes primers
T16110 514-515 . denotes .
T16111 515-613 sentence denotes Sequence analysis was done using Sequencher 4.1 (Gene Codes, Ann Arbor, Michigan, United States).
T16112 516-524 NN denotes Sequence
T16113 525-533 NN denotes analysis
T16115 534-537 VBD denotes was
T16114 538-542 VBN denotes done
T16116 543-548 VBG denotes using
T16117 549-559 NNP denotes Sequencher
T16118 560-563 CD denotes 4.1
T16119 564-565 -LRB- denotes (
T16121 565-569 NNP denotes Gene
T16120 570-575 NNPS denotes Codes
T16122 575-577 , denotes ,
T16123 577-580 NNP denotes Ann
T16124 581-586 NNP denotes Arbor
T16125 586-588 , denotes ,
T16126 588-596 NNP denotes Michigan
T16127 596-598 , denotes ,
T16128 598-604 NNP denotes United
T16129 605-611 NNP denotes States
T16130 611-612 -RRB- denotes )
T16131 612-613 . denotes .
R4590 T16011 T16012 nsubjpass RNA,extracted
R4591 T16013 T16012 auxpass was,extracted
R4592 T16014 T16012 prep by,extracted
R4593 T16015 T16016 amod standard,techniques
R4594 T16016 T16014 pobj techniques,by
R4595 T16017 T16012 prep from,extracted
R4596 T16018 T16019 amod thoracic,tissue
R4597 T16019 T16017 pobj tissue,from
R4598 T16020 T16019 amod embryonic,tissue
R4599 T16021 T16012 punct .,extracted
R4600 T16023 T16024 compound RT,PCR
R4601 T16024 T16026 nsubjpass PCR,performed
R4602 T16025 T16024 punct -,PCR
R4603 T16027 T16026 auxpass was,performed
R4604 T16028 T16026 advcl using,performed
R4605 T16029 T16030 nummod six,sets
R4606 T16030 T16028 dobj sets,using
R4607 T16031 T16030 compound primer,sets
R4608 T16032 T16030 acl designed,sets
R4609 T16033 T16034 aux to,cover
R4610 T16034 T16032 advcl cover,designed
R4611 T16035 T16036 det the,gene
R4612 T16036 T16034 dobj gene,cover
R4613 T16037 T16036 compound Fog2,gene
R4614 T16038 T16026 punct .,performed
R4615 T16040 T16041 compound RT,PCR
R4616 T16041 T16043 nsubjpass PCR,repeated
R4617 T16042 T16041 punct -,PCR
R4618 T16044 T16043 auxpass was,repeated
R4619 T16045 T16043 prep with,repeated
R4620 T16046 T16047 amod radiolabeled,primers
R4621 T16047 T16045 pobj primers,with
R4622 T16048 T16049 aux to,amplify
R4623 T16049 T16043 advcl amplify,repeated
R4624 T16050 T16051 det an,region
R4625 T16051 T16049 dobj region,amplify
R4626 T16052 T16053 advmod abnormally,spliced
R4627 T16053 T16051 amod spliced,region
R4628 T16054 T16051 prep of,region
R4629 T16055 T16056 det the,gene
R4630 T16056 T16054 pobj gene,of
R4631 T16057 T16058 punct (,S1
R4632 T16058 T16043 parataxis S1,repeated
R4633 T16059 T16058 compound Table,S1
R4634 T16060 T16058 punct ),S1
R4635 T16061 T16043 punct ", ",repeated
R4636 T16062 T16043 cc and,repeated
R4637 T16063 T16064 det the,product
R4638 T16064 T16065 nsubjpass product,run
R4639 T16065 T16043 conj run,repeated
R4640 T16066 T16065 auxpass was,run
R4641 T16067 T16065 prep on,run
R4642 T16068 T16069 det a,gel
R4643 T16069 T16067 pobj gel,on
R4644 T16070 T16069 amod denaturing,gel
R4645 T16071 T16069 compound sequencing,gel
R4646 T16072 T16065 prep according,run
R4647 T16073 T16072 prep to,according
R4648 T16074 T16075 amod standard,techniques
R4649 T16075 T16073 pobj techniques,to
R4650 T16076 T16065 punct .,run
R4651 T16078 T16079 det The,product
R4652 T16079 T16083 nsubjpass product,cloned
R4653 T16080 T16081 compound RT,PCR
R4654 T16081 T16079 compound PCR,product
R4655 T16082 T16081 punct -,PCR
R4656 T16084 T16083 auxpass was,cloned
R4657 T16085 T16083 prep into,cloned
R4658 T16086 T16087 compound pCR2.1,vector
R4659 T16087 T16085 pobj vector,into
R4660 T16088 T16083 advcl using,cloned
R4661 T16089 T16090 compound TOPO,Kit
R4662 T16090 T16088 dobj Kit,using
R4663 T16091 T16090 compound TA,Kit
R4664 T16092 T16090 compound Cloning,Kit
R4665 T16093 T16094 punct (,Invitrogen
R4666 T16094 T16090 parataxis Invitrogen,Kit
R4667 T16095 T16094 punct ", ",Invitrogen
R4668 T16096 T16094 npadvmod Carlsbad,Invitrogen
R4669 T16097 T16094 punct ", ",Invitrogen
R4670 T16098 T16094 npadvmod California,Invitrogen
R4671 T16099 T16094 punct ", ",Invitrogen
R4672 T16100 T16101 compound United,States
R4673 T16101 T16094 npadvmod States,Invitrogen
R4674 T16102 T16094 punct ),Invitrogen
R4675 T16103 T16083 cc and,cloned
R4676 T16104 T16083 conj sequenced,cloned
R4677 T16105 T16104 advcl using,sequenced
R4678 T16106 T16107 npadvmod gene,specific
R4679 T16107 T16109 amod specific,primers
R4680 T16108 T16107 punct -,specific
R4681 T16109 T16105 dobj primers,using
R4682 T16110 T16083 punct .,cloned
R4683 T16112 T16113 compound Sequence,analysis
R4684 T16113 T16114 nsubjpass analysis,done
R4685 T16115 T16114 auxpass was,done
R4686 T16116 T16114 advcl using,done
R4687 T16117 T16116 dobj Sequencher,using
R4688 T16118 T16117 nummod 4.1,Sequencher
R4689 T16119 T16120 punct (,Codes
R4690 T16120 T16117 parataxis Codes,Sequencher
R4691 T16121 T16120 compound Gene,Codes
R4692 T16122 T16120 punct ", ",Codes
R4693 T16123 T16124 compound Ann,Arbor
R4694 T16124 T16120 npadvmod Arbor,Codes
R4695 T16125 T16120 punct ", ",Codes
R4696 T16126 T16120 npadvmod Michigan,Codes
R4697 T16127 T16120 punct ", ",Codes
R4698 T16128 T16129 compound United,States
R4699 T16129 T16120 npadvmod States,Codes
R4700 T16130 T16120 punct ),Codes
R4701 T16131 T16114 punct .,done