PubMed_ArguminSci  

PubMed:23002246 JSONTXT 48 Projects

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Id Subject Object Predicate Lexical cue
T1 105-205 DRI_Background denotes Human embryonic stem (hES) cell production of heparan sulfate influences cell fate and pluripotency.
T2 206-408 DRI_Outcome denotes Human ES cells remain pluripotent in vitro through the action of growth factors signaling, and the activity of these factors depends on interaction with specific receptors and also with heparan sulfate.
T3 409-598 DRI_Approach denotes Here, we tested the hypothesis that matrix-associated heparan sulfate is enough to maintain hES cells under low fibroblast growth factor-2 concentration in the absence of live feeder cells.
T4 599-754 DRI_Background denotes To pursue this goal, we compared hES cells cultured either on coated plates containing live murine embryonic fibroblasts (MEFs) or on a matrix derived from
T5 773-774 DRI_Background denotes .
T6 775-882 DRI_Approach denotes hES cells were analyzed for the expression of pluripotency markers and the ability to form embryoid bodies.
T7 883-1084 DRI_Background denotes hES cells cultured either on live mouse fibroblasts or onto a matrix derived from fixed fibroblasts expressed similar levels of Oct-4, SOX-2, Nanog, TRA-1-60 and SSEA-4, and they were also able to form
T8 1104-1111 DRI_Background denotes bodies.
T9 1112-1339 DRI_Approach denotes Heparan sulfate-depleted matrix lost the ability to support the adherence and growth of hES cells, confirming that this glycosaminoglycan, bound to the extracellular matrix, is enough for the growth and attachment of hES cells.
T10 1340-1501 DRI_Approach denotes Finally, we observed that the ethanol-fixed matrix decreases by 30% the levels of Neu5Gc in hES cells, indicating that this procedure reduces xeno-contamination.
T11 1502-1618 DRI_Outcome denotes Our data suggest that matrix-bound heparan sulfate is required for the growth and pluripotency of hES cells and that
T12 1638-1697 DRI_Outcome denotes may be used as a "live cell"-free substrate for stem cells.