Epistemic_Statements  

CORD-19:2343e42a324724f6f6c6d3f76b564f41e4596261 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE-old TextAE

Id Subject Object Predicate Lexical cue
T1 716-1061 Epistemic_statement denotes The overall specificity of the Luminex xTAG RVP Fast v1 assay was slightly higher than that of the CLART® PneumoVir assay for human bocavirus, influenza A virus/H3N2, influenza B virus, human metapneumovirus, and parainfluenza virus, whereas the sensitivity of the latter was higher for most targeted viruses except, notably, for picornaviruses.
T2 1333-1528 Epistemic_statement denotes They can range from mild self-limiting illness to severe disease, the latter particularly in pediatric and severely immunosuppressed patients (Ison and Hayden, 2002; Vallières and Renaud, 2013) .
T3 1971-2087 Epistemic_statement denotes The decision to choose one or other molecular system is complex and requires systematic and comparative evaluations.
T4 2088-2656 Epistemic_statement denotes In this context, the Luminex xTAG RVP Fast assay (Luminex Molecular Diagnostics, Austin, TX, USA) has been extensively evaluated in recent years, and comparative studies have been also published showing high reliability for detection of 19 RVs in different clinical settings (Babady et al., 2012; Dabisch-Ruthe et al., 2012; Gadsby et al., 2010; Hwang et al., 2014; Jokela et al., 2012; Krunic et al., 2007; Merante et al., 2007; Pabbaraju et al., 2008 Pabbaraju et al., , 2011 Pillet et al., 2013; Popowitch et al., 2013; Rand et al., 2011; Raymaekers et al., 2011) .
T5 2657-2850 Epistemic_statement denotes The CLART® PneumoVir assay (Genomica, Coslada, Spain) is a reverse transcription-polymerase chain reaction (RT-PCR) DNA microarray method that makes it possible to detect simultaneously 17 RVs.
T6 2851-3069 Epistemic_statement denotes The reliability of this procedure for the detection of RVs in children and adults has been previously shown (Culebras et al., 2013; Frobert et al., 2011; Pillet et al., 2013; Renois et al., 2010; Tokman et al., 2014) .
T7 3070-3162 Epistemic_statement denotes Nevertheless, little is known as to how this system compares with other multiplex platforms.
T8 4671-4837 Epistemic_statement denotes Likewise, the NucliSENS® easyMAG™ method has been previously validated for its use coupled to the CLART® PneumoVir assay (Frobert et al., 2011; Pillet et al., 2013) .
T9 4838-5108 Epistemic_statement denotes In turn, the Qiagen EZ-1 Viral extraction kit has been validated by the manufacturer (personal communication) and by our group (unpublished results) against the manual nucleic acid extraction and purification method recommended by the manufacturer in the package insert.
T10 5406-5512 Epistemic_statement denotes Both leftover specimens and nucleic acid extracts were then conserved at −70°C for further investigations.
T11 5886-6233 Epistemic_statement denotes In order to determine whether long-term storage and repeated freezing and thawing (maximum, 3 cycles) had any effect on RVs detectability, 10 randomly selected nucleic acid extracts that were subjected to nested PCR sequencing for analysis of discrepancies were reanalyzed by both the Luminex xTAG RVP Fast v1 assay and the CLART® PneumoVir assay.
T12 8325-8464 Epistemic_statement denotes The CLART® PneumoVir DNA array assay (Genomica, Coslada, Spain) was performed and interpreted following the manufacturer's recommendations.
T13 10443-10583 Epistemic_statement denotes The latter were those in which both assays were coincident in at least 1 virus, although 1 assay could identify more viruses than the other.
T14 11788-11916 Epistemic_statement denotes Likewise, both methods performed comparably for detection of multiple infections (n = 29 versus n = 27, respectively; P = 0.94).
T15 12646-12729 Epistemic_statement denotes It is of note that InfA H1N1/v was detected by the CLART® PneumoVir in 5 specimens.
T16 13339-13554 Epistemic_statement denotes In contrast, the CLART® PneumoVir method appeared to perform slightly better for detection of most of the other RVs included in the panels, although the differences did not reach statistical significance (Table 3 ).
T17 14059-14300 Epistemic_statement denotes In the remaining 6 specimens, the presence of 1 or more viral agents in respiratory specimens, as detected by one or the other method, could not be confirmed by the reference method and thus were considered false-positive results (Table 2) .
T18 14301-14517 Epistemic_statement denotes Following resolution of discrepancies, the data indicated that the sensitivity of the Luminex xTAG RVP Fast v1 assay was higher than of the CLART® PneumoVir assay for detection of picornaviruses (89.8% versus 59.3%).
T19 15125-15194 Epistemic_statement denotes It is of note that this version is no longer available in the market.
T20 15675-15844 Epistemic_statement denotes ii) The sensitivity of the CLART® PneumoVir assay was higher than that of the Luminex xTAG RVP Fast v1 assay for most targeted agents, except notably for picornaviruses.
T21 16970-17162 Epistemic_statement denotes This has also been reported in other studies (Pillet et al., 2013; Tokman et al., 2014) and may likely be due to the fact that the assay only detects enterovirus type B (Pillet et al., 2013) .
T22 17163-17343 Epistemic_statement denotes False-positive results were scarce, and most frequently obtained with the CLART® PneumoVir assay, especially for hBoV and some PIVs, and notably in the context of mixed infections.
T23 17344-17418 Epistemic_statement denotes Mispriming is a likely reason accounting for these false-positive results.
T24 17419-17644 Epistemic_statement denotes Nevertheless, given the high overall sensitivity of CLART® PneumoVir, we cannot rule out the possibility of these being true positives that remained undetected by the nested PCR assay employed for resolution of discrepancies.
T25 17645-17738 Epistemic_statement denotes This is in contrast to previously published data (Pillet et al., 2013; Tokman et al., 2014) .
T26 17739-17856 Epistemic_statement denotes The use of different criteria for resolving discordant results among the studies may account for these discrepancies.
T27 17857-18115 Epistemic_statement denotes Limitations of the current study are the relatively scarce number of positive specimens for several viral agents, and its retrospective design, which implied the use of thawed instead of freshly obtained specimens for testing with the CLART® PneumoVir assay.
T28 18405-18652 Epistemic_statement denotes Despite the above limitations, our data proved the reliability of the CLART® PneumoVir assay as compared to the Luminex xTAG RVP Fast v1 assay for the diagnosis of upper and lower tract respiratory infections, both in adult and pediatric patients.