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Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-141 Sentence denotes [Influence of human amniotic mesenchymal stem cells on macrophage phenotypes and inflammatory factors in full-thickness skin wounds of mice].
T2 142-152 Sentence denotes Objective:
T3 153-385 Sentence denotes To explore the influence of human amniotic mesenchymal stem cells (hAMSCs) on the in vivo and in vitro regulation of macrophage phenotypes and inflammatory factors associated with wound healing of full-thickness skin wounds in mice.
T4 386-394 Sentence denotes Methods:
T5 395-659 Sentence denotes Fresh amniotic membrane discarded from full-term delivery by 5 healthy pregnant women in the Department of Obstetrics and Gynecology of the Affiliated Hospital of Zunyi Medical University was used for the isolation and culture of hAMSCs by enzyme digestion method.
T6 660-760 Sentence denotes The third passage of cells was used for identification of adipogenic and osteogenic differentiation.
T7 761-843 Sentence denotes The fourth passage of cells was used for identification of hAMSCs surface markers.
T8 844-1118 Sentence denotes Ten C57BL/6 mice (all male, aged 6 to 8 weeks, the same gender and age below) were selected for extracting mouse peritoneal macrophages by intraperitoneal lavage, and M1-type macrophages were induced by Dulbecco's modified eagle medium (DMEM) medium containing interferon-γ.
T9 1119-1213 Sentence denotes The M1-type macrophages were divided into hAMSCs+ macrophage group and macrophage alone group.
T10 1214-1367 Sentence denotes Then 1×10(4) hAMSCs/per well of fourth passage were added to macrophage in hAMSCs+ macrophage group and cultured in 2 mL DMEM medium for routine culture.
T11 1368-1462 Sentence denotes In macrophage alone group, each well was only added with 2 mL DMEM medium for routine culture.
T12 1463-1905 Sentence denotes On day 1 and 7 in culture, the content of interleukin-12 (IL-12), arginase 1, and IL-10 in the cell culture supernatant of the 2 groups were detected by enzyme-linked immunosorbent assay with sample number of 6/per group. (2) Full-thickness skin wound model was reproduced in the back of 56 C57BL/6 mice, which were divided into hAMSCs group and phosphate buffer solution (PBS) group using the random number table, with 28 mice in each group.
T13 1906-2059 Sentence denotes Mice in hAMSCs group were subcutaneously injected with 100 μL of cell suspension containing 1×10(7) hAMSCs per mL in PBS suspension along the wound edge.
T14 2060-2155 Sentence denotes While mice in PBS group were only subcutaneously injected with 100 μL PBS along the wound edge.
T15 2156-2255 Sentence denotes On post injection day (PID) 1, 3, 7, and 14, 7 mice in the two groups were sacrificed respectively.
T16 2256-2332 Sentence denotes Histopathological observation was performed with hematoxylin-eosin staining.
T17 2333-2549 Sentence denotes The expressions of macrophage surface markers [CD68 and inducible nitric oxide synthase (iNOS) double positive cells and CD68 and arginase 1 double positive] in the wounds were detected by immunofluorescent staining.
T18 2550-2750 Sentence denotes The mRNA expressions of IL-10, macrophage inflammatory protein 1α (MIP-1α), and MIP-2 in the wounds were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction.
T19 2751-2866 Sentence denotes Data were statistically analyzed with analysis of variance for factorial design, t test, and Bonferroni correction.
T20 2867-3190 Sentence denotes Results: (1) On day 1 in culture, the content of IL-12 and arginase 1 in the cell culture supernatant of the two groups were similar (t=0.448, 0.536, P>0.05), and the content of IL-10 in the cell culture supernatant of hAMSCs+ macrophage group was significantly lower than that in macrophage alone group (t=14.722, P<0.01).
T21 3191-3614 Sentence denotes On day 7 in culture, the content of IL-12 in the cell culture supernatant of hAMSCs+ macrophage group was significantly lower than that in macrophage alone group (t=13.226, P<0.01), and the content of arginase 1 and IL-10 was significantly higher than that in macrophage alone group (t=30.172, 31.406, P<0.01). (2) On PID 1, a large number of inflammatory cells infiltration were observed in the skin wounds of both groups.
T22 3615-3798 Sentence denotes On PID 3, the inflammatory cells infiltration in the skin wounds increased in both groups, and the inflammatory cells infiltration in hAMSCs group was less than that in the PBS group.
T23 3799-3977 Sentence denotes On PID 7, the inflammatory cells infiltration in the wounds decreased in both groups, and the inflammatory cells infiltration in hAMSCs group was less than that in the PBS group.
T24 3978-4297 Sentence denotes On PID 14, no obvious inflammatory cells infiltration was observed in the wounds in the two groups. (3) On PID 1 and 14, the percentages of CD68 and iNOS double positive cells and CD68 and arginase 1 double positive cells in the wounds were similar in the two groups (t(1 d)=0.134, 0.693, t(14 d)=1.146, 2.585, P>0.05).
T25 4298-4732 Sentence denotes On PID 3 and 7, the percentages of CD68 and iNOS double positive cells in the wounds in hAMSCs group were significantly lower than those of PBS group (t=6.396, 4.787, P<0.01), while the percentages of CD68 and arginase 1 double positive cells were significantly higher than those of PBS group (t=3.928, 4.473, P<0.01). (4) On PID 1, the mRNA expressions of IL-10 in the wounds of mice in the two groups were similar (t=2.005, P>0.05).
T26 4733-4907 Sentence denotes On PID 3, 7, and 14, the mRNA expressions of IL-10 in the wounds of mice in hAMSCs group were significantly higher than those of PBS group (t=7.758, 124.355, 80.823, P<0.01).
T27 4908-5349 Sentence denotes On PID 1, 3, 7, and 14, the mRNA expressions of MIP-1α and MIP-2 in the wounds of mice in hAMSCs group (0.341±0.212, 0.648±0.004, 0.611±0.106, 0.763±0.049, 1.377±0.099, 1.841±0.042, 1.181±0.035, 0.553±0.028) were significantly lower than those of PBS group (3.853±0.035, 6.914±0.163, 3.648±0.113, 2.250±0.046, 11.119±0.495, 8.634±0.092, 5.722±0.021, 4.862±0.036, t=43.198, 101.904, 51.845, 58.231, 51.074, 177.501, 291.752, 251.614, P<0.01).
T28 5350-5520 Sentence denotes Conclusions: hAMSCs demonstrates biological effects of promoting the transformation of M1-type macrophages into M2-type macrophages in full-thickness skin wounds of mice.
T29 5521-5703 Sentence denotes They can up-regulate the expression of anti-inflammatory and anti-fibrotic factor IL-10, and down-regulate the expression of important inflammation mediated factors MIP-1α and MIP-2.
T1 0-141 Sentence denotes [Influence of human amniotic mesenchymal stem cells on macrophage phenotypes and inflammatory factors in full-thickness skin wounds of mice].
T2 142-152 Sentence denotes Objective:
T3 153-385 Sentence denotes To explore the influence of human amniotic mesenchymal stem cells (hAMSCs) on the in vivo and in vitro regulation of macrophage phenotypes and inflammatory factors associated with wound healing of full-thickness skin wounds in mice.
T4 386-394 Sentence denotes Methods:
T5 395-659 Sentence denotes Fresh amniotic membrane discarded from full-term delivery by 5 healthy pregnant women in the Department of Obstetrics and Gynecology of the Affiliated Hospital of Zunyi Medical University was used for the isolation and culture of hAMSCs by enzyme digestion method.
T6 660-760 Sentence denotes The third passage of cells was used for identification of adipogenic and osteogenic differentiation.
T7 761-843 Sentence denotes The fourth passage of cells was used for identification of hAMSCs surface markers.
T8 844-1118 Sentence denotes Ten C57BL/6 mice (all male, aged 6 to 8 weeks, the same gender and age below) were selected for extracting mouse peritoneal macrophages by intraperitoneal lavage, and M1-type macrophages were induced by Dulbecco's modified eagle medium (DMEM) medium containing interferon-γ.
T9 1119-1213 Sentence denotes The M1-type macrophages were divided into hAMSCs+ macrophage group and macrophage alone group.
T10 1214-1367 Sentence denotes Then 1×10(4) hAMSCs/per well of fourth passage were added to macrophage in hAMSCs+ macrophage group and cultured in 2 mL DMEM medium for routine culture.
T11 1368-1462 Sentence denotes In macrophage alone group, each well was only added with 2 mL DMEM medium for routine culture.
T12 1463-1905 Sentence denotes On day 1 and 7 in culture, the content of interleukin-12 (IL-12), arginase 1, and IL-10 in the cell culture supernatant of the 2 groups were detected by enzyme-linked immunosorbent assay with sample number of 6/per group. (2) Full-thickness skin wound model was reproduced in the back of 56 C57BL/6 mice, which were divided into hAMSCs group and phosphate buffer solution (PBS) group using the random number table, with 28 mice in each group.
T13 1906-2059 Sentence denotes Mice in hAMSCs group were subcutaneously injected with 100 μL of cell suspension containing 1×10(7) hAMSCs per mL in PBS suspension along the wound edge.
T14 2060-2155 Sentence denotes While mice in PBS group were only subcutaneously injected with 100 μL PBS along the wound edge.
T15 2156-2255 Sentence denotes On post injection day (PID) 1, 3, 7, and 14, 7 mice in the two groups were sacrificed respectively.
T16 2256-2332 Sentence denotes Histopathological observation was performed with hematoxylin-eosin staining.
T17 2333-2549 Sentence denotes The expressions of macrophage surface markers [CD68 and inducible nitric oxide synthase (iNOS) double positive cells and CD68 and arginase 1 double positive] in the wounds were detected by immunofluorescent staining.
T18 2550-2750 Sentence denotes The mRNA expressions of IL-10, macrophage inflammatory protein 1α (MIP-1α), and MIP-2 in the wounds were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction.
T19 2751-2866 Sentence denotes Data were statistically analyzed with analysis of variance for factorial design, t test, and Bonferroni correction.
T20 2867-3190 Sentence denotes Results: (1) On day 1 in culture, the content of IL-12 and arginase 1 in the cell culture supernatant of the two groups were similar (t=0.448, 0.536, P>0.05), and the content of IL-10 in the cell culture supernatant of hAMSCs+ macrophage group was significantly lower than that in macrophage alone group (t=14.722, P<0.01).
T21 3191-3614 Sentence denotes On day 7 in culture, the content of IL-12 in the cell culture supernatant of hAMSCs+ macrophage group was significantly lower than that in macrophage alone group (t=13.226, P<0.01), and the content of arginase 1 and IL-10 was significantly higher than that in macrophage alone group (t=30.172, 31.406, P<0.01). (2) On PID 1, a large number of inflammatory cells infiltration were observed in the skin wounds of both groups.
T22 3615-3798 Sentence denotes On PID 3, the inflammatory cells infiltration in the skin wounds increased in both groups, and the inflammatory cells infiltration in hAMSCs group was less than that in the PBS group.
T23 3799-3977 Sentence denotes On PID 7, the inflammatory cells infiltration in the wounds decreased in both groups, and the inflammatory cells infiltration in hAMSCs group was less than that in the PBS group.
T24 3978-4297 Sentence denotes On PID 14, no obvious inflammatory cells infiltration was observed in the wounds in the two groups. (3) On PID 1 and 14, the percentages of CD68 and iNOS double positive cells and CD68 and arginase 1 double positive cells in the wounds were similar in the two groups (t(1 d)=0.134, 0.693, t(14 d)=1.146, 2.585, P>0.05).
T25 4298-4732 Sentence denotes On PID 3 and 7, the percentages of CD68 and iNOS double positive cells in the wounds in hAMSCs group were significantly lower than those of PBS group (t=6.396, 4.787, P<0.01), while the percentages of CD68 and arginase 1 double positive cells were significantly higher than those of PBS group (t=3.928, 4.473, P<0.01). (4) On PID 1, the mRNA expressions of IL-10 in the wounds of mice in the two groups were similar (t=2.005, P>0.05).
T26 4733-4907 Sentence denotes On PID 3, 7, and 14, the mRNA expressions of IL-10 in the wounds of mice in hAMSCs group were significantly higher than those of PBS group (t=7.758, 124.355, 80.823, P<0.01).
T27 4908-5349 Sentence denotes On PID 1, 3, 7, and 14, the mRNA expressions of MIP-1α and MIP-2 in the wounds of mice in hAMSCs group (0.341±0.212, 0.648±0.004, 0.611±0.106, 0.763±0.049, 1.377±0.099, 1.841±0.042, 1.181±0.035, 0.553±0.028) were significantly lower than those of PBS group (3.853±0.035, 6.914±0.163, 3.648±0.113, 2.250±0.046, 11.119±0.495, 8.634±0.092, 5.722±0.021, 4.862±0.036, t=43.198, 101.904, 51.845, 58.231, 51.074, 177.501, 291.752, 251.614, P<0.01).
T28 5350-5520 Sentence denotes Conclusions: hAMSCs demonstrates biological effects of promoting the transformation of M1-type macrophages into M2-type macrophages in full-thickness skin wounds of mice.
T29 5521-5703 Sentence denotes They can up-regulate the expression of anti-inflammatory and anti-fibrotic factor IL-10, and down-regulate the expression of important inflammation mediated factors MIP-1α and MIP-2.